1. Structural Insights into Dopamine Receptor-Ligand Interactions: From Agonists to Antagonists.
- Author
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Barbosa ED, Ma Y, Clift HE, Olson LJ, Zhu L, and Liu W
- Subjects
- Humans, Ligands, Receptors, Dopamine D1 metabolism, Receptors, Dopamine D1 agonists, Tetrahydronaphthalenes pharmacology, Tetrahydronaphthalenes chemistry, Receptors, Dopamine D5 agonists, Receptors, Dopamine D5 metabolism, Thiophenes pharmacology, Thiophenes chemistry, Protein Binding physiology, Binding Sites, Benzazepines pharmacology, Benzazepines chemistry, Models, Molecular, 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine pharmacology, 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine analogs & derivatives, Dopamine Agonists pharmacology, Dopamine Agonists chemistry, Dopamine Antagonists pharmacology, Dopamine Antagonists chemistry
- Abstract
This study explores the intricacies of dopamine receptor-ligand interactions, focusing on the D1R and D5R subtypes. Using molecular modeling techniques, we investigated the binding of the pan-agonist rotigotine, revealing a universal binding mode at the orthosteric binding pocket. Additionally, we analyze the stability of antagonist-receptor complexes with SKF83566 and SCH23390. By examining the impact of specific mutations on ligand-receptor interactions through computational simulations and thermostability assays, we gain insights into binding stability. Our research also delves into the structural and energetic aspects of antagonist binding to D1R and D5R in their inactive states. These findings enhance our understanding of dopamine receptor pharmacology and hold promise for drug development in central nervous system disorders, opening doors to future research and innovation in this field.
- Published
- 2024
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