RP-HPLC analysis of diterpene lactones in leaves and stem of different species of Andrographis .

In the present study diterpene lactones were quantified in leaves and stem of different species of Andrographis collected from Western Ghats of India using reverse phase high performance liquid chromatography (RP-HPLC) method. Different populations of AA ( Andrographis alata ), AE ( Andrographis echioides ), ALn ( Andrographis lineata var. lineata ), ALw ( Andrographis lineata var. lawii ), AM ( Andrographis macrobotrys ), AO ( Andrographis ovata ), AP ( Andrographis paniculata ), APr ( Andrographis producta ) and AS ( Andrographis serphyllifolia ) were assessed for the amount of AG (andrographolide), NAG (neoandrographolide) and DDAG (14-deoxy-11, 12-didehydroandrographolide) in leaves and stem. The most abundant diterpenoid was AG and highest amount of 68.35 mg/g DW was recorded in a population of AP. AG was also present in leaves of ALw at considerable level (40.85 mg/g DW). NAG was optimum in the leaves of AM (98.43 to 102.03 mg/g DW). DDAG was higher in the leaves of AP (16.01 mg/g DW).