30 results on '"Tawde S"'
Search Results
2. Death by Caffeine: Presumptive Malicious Poisoning of a Dog by Incorporation in Ground Meat
- Author
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Tawde, S. N., Puschner, B., Albin, T., Stump, S., and Poppenga, R. H.
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- 2012
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3. Use of a reduced-intensity conditioning regimen for allogeneic transplantation in patients with chronic myeloid leukemia
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Das, M, Saikia, T K, Advani, S H, Parikh, P M, and Tawde, S
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- 2003
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4. Estimation of Glacier Mass Balance on a Basin Scale:An Approach Based on Satellite-Derived Snowlines and a Temperature Index Model
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Tawde, S. A., primary, Kulkarni, A. V., additional, and Bala, G., additional
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- 2016
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5. Use of a reduced-intensity conditioning regimen for allogeneic transplantation in patients with chronic myeloid leukemia
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Purvish M. Parikh, Tapan K. Saikia, Manjulika Das, Tawde S, and Suresh H. Advani
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Adult ,Male ,medicine.medical_specialty ,Allogeneic transplantation ,Transplantation Conditioning ,Adolescent ,medicine.medical_treatment ,Radiation Dosage ,Donor lymphocyte infusion ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,medicine ,Humans ,Transplantation, Homologous ,Immunosuppression Therapy ,Transplantation ,business.industry ,Hematopoietic Stem Cell Transplantation ,Immunosuppression ,Hematology ,medicine.disease ,Survival Analysis ,Surgery ,Fludarabine ,Hospitalization ,Regimen ,surgical procedures, operative ,Graft-versus-host disease ,Treatment Outcome ,Female ,business ,Busulfan ,Immunosuppressive Agents ,Whole-Body Irradiation ,medicine.drug - Abstract
Reduced-intensity conditioning that harnesses the potential of a graft-versus-tumor (GVT) effect has been proposed as an alternative to conventional myeloablative allogeneic stem cell transplantation. The primary aim is engraftment and this can be achieved with minimal immunosuppression. In this report, we describe the use of such regimens for CML in 17 patients who received human leukocyte antigen (HLA)-matched sibling allografts. Conditioning was with fludarabine, antithymocyte globulin (ATG) and busulfan for the first 11 patients, whereas fludarabine, busulfan and TBI were used for the remaining six patients. Engraftment was prompt in most of the cases. Complications and need for supportive therapy in the immediate post-transplant period were reduced drastically. Only two patients (both in the TBI group) died within the first 100 days. Acute graft-versus-host disease (GVHD) grade II-IV was seen in seven patients. Complications occurred later on. Chronic GVHD was observed in 11/17 patients. Lung infection and GVHD were the major killers. In surviving patients, after a median follow-up of 30 months (range 37-21 months), 6/17 (35.3%) are alive. Five are disease free and one patient is still in relapse even after a second donor lymphocyte infusion. Total treatment time and cost were more than with conventional transplants. We conclude that reduced-intensity transplantation still requires further refinement.
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- 2003
6. Consensus coding: a novel method of evaluating scarring after cleft lip repair
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Tawde, S., primary, Simmons, D.R., additional, Bell, A., additional, Moos, K., additional, Naudi, K., additional, and Ayoub, A., additional
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- 2011
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7. Consensus Coding as a Tool in Visual Appearance Research
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Simmons, D R, primary, Tawde, S, additional, and Ayoub, A F, additional
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- 2011
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8. Antacids in the initial management of metallic zinc ingestion in dogs
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van der MERWE, D., primary and TAWDE, S., additional
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- 2009
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9. Redox behavior of polyaniline as influenced by aromatic sulphonate anions: cyclic voltammetry and molecular modeling
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Tawde, S., Mukesh, D., and Yakhmi, J. V.
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- 2001
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10. Clinical, Radiological and Pathological Features of a Large American Cohort of Spinocerebellar Ataxia (SCA27B).
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Abou Chaar W, Eranki AN, Stevens HA, Watson SL, Wong DY, Avila VS, Delfeld M, Gary AJ, Tawde S, Triebold M, Cherchi M, Xie T, Lockhart PJ, Bahlo M, Pellerin D, Dicaire MJ, Danzi M, Zuchner S, Brais BC, Perlman S, Burmeister M, Paulson H, Srinivasan S, Schut L, Bower M, Bushara K, Liao C, Shakkottai VG, Collins J, Clark HB, Das S, Fogel BL, and Gomez CM
- Abstract
Objectives: Spinocerebellar ataxia 27B due to GAA repeat expansions in the fibroblast growth factor 14 (FGF14) gene has recently been recognized as a common cause of late-onset hereditary cerebellar ataxia. Here we present the first report of this disease in the US population, characterizing its clinical manifestations, disease progression, pathological abnormalities, and response to 4-aminopyridine in a cohort of 102 patients bearing GAA repeat expansions., Methods: We compiled a series of patients with SCA27B, recruited from 5 academic centers across the United States. Clinical manifestations and patient demographics were collected retrospectively from clinical records in an unblinded approach using a standardized form. Post-mortem analysis was done on 4 brains of patients with genetically confirmed SCA27B., Results: In our cohort of 102 patients with SCA27B, we found that SCA27B was a late-onset (57 ± 12.5 years) slowly progressive ataxia with an episodic component in 51% of patients. Balance and gait impairment were almost always present at disease onset. The principal finding on post-mortem examination of 4 brain specimens was loss of Purkinje neurons that was most severe in the vermis most particularly in the anterior vermis. Similar to European populations, a high percent of patients 21/28 (75%) reported a positive treatment response with 4-aminopyridine., Interpretation: Our study further estimates prevalence and further expands the clinical, imaging and pathological features of SCA27B, while looking at treatment response, disease progression, and survival in patients with this disease. Testing for SCA27B should be considered in all undiagnosed ataxia patients, especially those with episodic onset. ANN NEUROL 2024., (© 2024 The Author(s). Annals of Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.)
- Published
- 2024
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11. Clinical and genetic delineation of autosomal recessive and dominant ACTL6B-related developmental brain disorders.
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Cali E, Quirin T, Rocca C, Efthymiou S, Riva A, Marafi D, Zaki MS, Suri M, Dominguez R, Elbendary HM, Alavi S, Abdel-Hamid MS, Morsy H, Mau-Them FT, Nizon M, Tesner P, Ryba L, Zafar F, Rana N, Saadi NW, Firoozfar Z, Gencpinar P, Unay B, Ustun C, Bruel AL, Coubes C, Stefanich J, Sezer O, Agolini E, Novelli A, Vasco G, Lettori D, Milh M, Villard L, Zeidler S, Opperman H, Strehlow V, Issa MY, El Khassab H, Chand P, Ibrahim S, Nejad-Rashidi A, Miryounesi M, Larki P, Morrison J, Cristian I, Thiffault I, Bertsch NL, Noh GJ, Pappas J, Moran E, Marinakis NM, Traeger-Synodinos J, Hosseini S, Abbaszadegan MR, Caumes R, Vissers LELM, Neshatdoust M, Montazer MZ, El Fahime E, Canavati C, Kamal L, Kanaan M, Askander O, Voinova V, Levchenko O, Haider S, Halbach SS, Maia ER, Mansoor S, Vivek J, Tawde S, Santhosh R Challa V, Gowda VK, Srinivasan VM, Victor LA, Pinero-Banos B, Hague J, Ei-Awady HA, Maria de Miranda Henriques-Souza A, Cheema HA, Anjum MN, Idkaidak S, Alqarajeh F, Atawneh O, Mor-Shaked H, Harel T, Zifarelli G, Bauer P, Kok F, Kitajima JP, Monteiro F, Josahkian J, Lesca G, Chatron N, Ville D, Murphy D, Neul JL, Mullegama SV, Begtrup A, Herman I, Mitani T, Posey JE, Tay CG, Javed I, Carr L, Kanani F, Beecroft F, Hane L, Abdelkreem E, Macek M, Bispo L, Elmaksoud MA, Hashemi-Gorji F, Pehlivan D, Amor DJ, Jamra RA, Chung WK, Ghayoor EK, Campeau P, Alkuraya FS, Pagnamenta AT, Gleeson J, Lupski JR, Striano P, Moreno-De-Luca A, Lafontaine DLJ, Houlden H, and Maroofian R
- Abstract
Purpose: This study aims to comprehensively delineate the phenotypic spectrum of ACTL6B-related disorders, previously associated with both autosomal recessive and autosomal dominant neurodevelopmental disorders. Molecularly, the role of the nucleolar protein ACTL6B in contributing to the disease has remained unclear., Methods: We identified 105 affected individuals, including 39 previously reported cases, and systematically analysed detailed clinical and genetic data for all individuals. Additionally, we conducted knockdown experiments in neuronal cells to investigate the role of ACTL6B in ribosome biogenesis., Results: Biallelic variants in ACTL6B are associated with severe-to-profound global developmental delay/intellectual disability (GDD/ID), infantile intractable seizures, absent speech, autistic features, dystonia, and increased lethality. De novo monoallelic variants result in moderate-to-severe GDD/ID, absent speech, and autistic features, while seizures and dystonia were less frequently observed. Dysmorphic facial features and brain abnormalities, including hypoplastic corpus callosum, parenchymal volume loss/atrophy, are common findings in both groups. We reveal that in the nucleolus, ACTL6B plays a crucial role in ribosome biogenesis, in particular in pre-rRNA processing., Conclusion: This study provides a comprehensive characterization of the clinical spectrum of both autosomal recessive and dominant forms of ACTL6B-associated disorders. It offers a comparative analysis of their respective phenotypes provides a plausible molecular explanation and suggests their inclusion within the expanding category of 'ribosomopathies'., (Copyright © 2024. Published by Elsevier Inc.)
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- 2024
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12. Performance Evaluation of a Novel Combination of Four- and Five-Carbon [Butyric and Valeric] Short-Chain Fatty Acid Glyceride Esters in Broilers.
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Gracia MI, Vazquez P, Ibáñez-Pernía Y, Pos J, and Tawde S
- Abstract
A novel combination of Butyric and Valeric acid glycerol esters with oregano oil in a dry powder form was evaluated for performance improvements in broilers. The dosing regimen (500 g/Ton feed in starter and grower; 250 g/Ton in finisher feed) was considered low compared to conventional practices using non-esterified Butyric and Valeric short-chain fatty acids (SCFA). Six trials were conducted at various trial facilities in Italy, United Kingdom, Spain, and Poland. Supplemented broilers weighed significantly more than the control birds at 28 days of age (+3.4%; 1459 g vs. 1412 g; p = 0.0006) and at 42 days of age (+2.5%; 2834 g vs. 2763 g; p = 0.0030). Supplementation significantly reduced mortality from 1.9% to 0.8% during the finisher phase (from 29 to 42 days of age); however, average mortality was 3.2% for the whole 42-day growth period and was not affected. Further, supplemented broilers grew more (66.4 vs. 64.5 g/day; p = 0.0005), ate more feed (104.7 vs. 103.1 g/day; p = 0.0473), converted feed significantly more efficiently (1.58 vs. 1.60; p = 0.0072), leading to better EPEF value (410 vs. 389; p = 0.0006) than the control broilers. Meta-analysed trial performance data for novel SCFA formulations such as these are not commonly available, and serve to facilitate efficacy determination from an end-user perspective. The use of short- and medium-chain fatty acid esters in optimal low-dose combinations to reliably augment gut health and performance appears promising in commercial broiler production, and may lead to further improvements in industry practices and reduced antibiotic use.
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- 2024
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13. Germline Variants Incidentally Detected via Tumor-Only Genomic Profiling of Patients With Mesothelioma.
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Mitchell OD, Gilliam K, Del Gaudio D, McNeely KE, Smith S, Acevedo M, Gaduraju M, Hodge R, Ramsland ASS, Segal J, Das S, Hathaway F, Bryan DS, Tawde S, Galasinski S, Wang P, Tjota MY, Husain AN, Armato SG 3rd, Donington J, Ferguson MK, Turaga K, Churpek JE, Kindler HL, and Drazer MW
- Subjects
- Humans, Male, Middle Aged, Female, Genetic Predisposition to Disease, High-Throughput Nucleotide Sequencing, Genomics, Adaptor Proteins, Signal Transducing genetics, DNA Helicases genetics, Mesothelioma diagnosis, Mesothelioma genetics, Mesothelioma, Malignant
- Abstract
Importance: Patients with mesothelioma often have next-generation sequencing (NGS) of their tumor performed; tumor-only NGS may incidentally identify germline pathogenic or likely pathogenic (P/LP) variants despite not being designed for this purpose. It is unknown how frequently patients with mesothelioma have germline P/LP variants incidentally detected via tumor-only NGS., Objective: To determine the prevalence of incidental germline P/LP variants detected via tumor-only NGS of mesothelioma., Design, Setting, and Participants: A series of 161 unrelated patients with mesothelioma from a high-volume mesothelioma program had tumor-only and germline NGS performed during April 2016 to October 2021. Follow-up ranged from 18 months to 7 years. Tumor and germline assays were compared to determine which P/LP variants identified via tumor-only NGS were of germline origin. Data were analyzed from January to March 2023., Main Outcomes and Measures: The proportion of patients with mesothelioma who had P/LP germline variants incidentally detected via tumor-only NGS., Results: Of 161 patients with mesothelioma, 105 were male (65%), the mean (SD) age was 64.7 (11.2) years, and 156 patients (97%) self-identified as non-Hispanic White. Most (126 patients [78%]) had at least 1 potentially incidental P/LP germline variant. The positive predictive value of a potentially incidental germline P/LP variant on tumor-only NGS was 20%. Overall, 26 patients (16%) carried a P/LP germline variant. Germline P/LP variants were identified in ATM, ATR, BAP1, CHEK2, DDX41, FANCM, HAX1, MRE11A, MSH6, MUTYH, NF1, SAMD9L, and TMEM127., Conclusions and Relevance: In this case series of 161 patients with mesothelioma, 16% had confirmed germline P/LP variants. Given the implications of a hereditary cancer syndrome diagnosis for preventive care and familial counseling, clinical approaches for addressing incidental P/LP germline variants in tumor-only NGS are needed. Tumor-only sequencing should not replace dedicated germline testing. Universal germline testing is likely needed for patients with mesothelioma.
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- 2023
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14. Truncating Variants in RFC1 in Cerebellar Ataxia, Neuropathy, and Vestibular Areflexia Syndrome.
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Ronco R, Perini C, Currò R, Dominik N, Facchini S, Gennari A, Simone R, Stuart S, Nagy S, Vegezzi E, Quartesan I, El-Saddig A, Lavin T, Tucci A, Szymura A, Novis De Farias LE, Gary A, Delfeld M, Kandikatla P, Niu N, Tawde S, Shaw J, Polke J, Reilly MM, Wood NW, Crespan E, Gomez C, Chen JYH, Schmahmann JD, Gosal D, Houlden H, Das S, and Cortese A
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- Adult, Humans, Syndrome, Cerebellar Ataxia genetics, Cerebellar Ataxia diagnosis, Bilateral Vestibulopathy genetics, Bilateral Vestibulopathy diagnosis, Neurodegenerative Diseases, Vestibular Diseases genetics, Peripheral Nervous System Diseases
- Abstract
Background and Objective: Cerebellar ataxia, neuropathy, and vestibular areflexia syndrome (CANVAS) is an autosomal recessive neurodegenerative disease characterized by adult-onset and slowly progressive sensory neuropathy, cerebellar dysfunction, and vestibular impairment. In most cases, the disease is caused by biallelic (AAGGG)
n repeat expansions in the second intron of the replication factor complex subunit 1 ( RFC1 ). However, a small number of cases with typical CANVAS do not carry the common biallelic repeat expansion. The objective of this study was to expand the genotypic spectrum of CANVAS by identifying sequence variants in RFC1 -coding region associated with this condition., Methods: Fifteen individuals diagnosed with CANVAS and carrying only 1 heterozygous (AAGGG)n expansion in RFC1 underwent whole-genome sequencing or whole-exome sequencing to test for the presence of a second variant in RFC1 or other unrelated gene. To assess the effect of truncating variants on RFC1 expression, we tested the level of RFC1 transcript and protein on patients' derived cell lines., Results: We identified 7 patients from 5 unrelated families with clinically defined CANVAS carrying a heterozygous (AAGGG)n expansion together with a second truncating variant in trans in RFC1 , which included the following: c.1267C>T (p.Arg423Ter), c.1739_1740del (p.Lys580SerfsTer9), c.2191del (p.Gly731GlufsTer6), and c.2876del (p.Pro959GlnfsTer24). Patient fibroblasts containing the c.1267C>T (p.Arg423Ter) or c.2876del (p.Pro959GlnfsTer24) variants demonstrated nonsense-mediated mRNA decay and reduced RFC1 transcript and protein., Discussion: Our report expands the genotype spectrum of RFC1 disease. Full RFC1 sequencing is recommended in cases affected by typical CANVAS and carrying monoallelic (AAGGG)n expansions. In addition, it sheds further light on the pathogenesis of RFC1 CANVAS because it supports the existence of a loss-of-function mechanism underlying this complex neurodegenerative condition., (Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.)- Published
- 2023
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15. Understanding and interpretation of a variant of uncertain significance (VUS) genetic test result by pediatric providers who do not specialize in genetics.
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Menke C, Nagaraj CB, Dawson B, He H, Tawde S, and Wakefield EG
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- Child, Genetic Predisposition to Disease, Humans, Genetic Testing methods, Knowledge
- Abstract
The advancement of genetic testing technologies has allowed for better diagnosis and management of patients, but also results in more variants of uncertain significance (VUSs) due to the increased number of genes being analyzed. There are more genetic tests available and more providers who do not specialize in genetics ordering genetic testing, but few studies examining how providers who do not specialize in genetics interpret VUSs. This study surveyed pediatric providers at a midwestern pediatric care center who do not specialize in genetics about their understanding of a mock genetic test report with a VUS result and whether their understanding of the result was associated with experience ordering genetic tests. Participants' preferences about content of the report and steps taken to understand the result were also examined. Of the 51 participants, 33% correctly answered both knowledge questions about the VUS result: one asking them to interpret the result and one asking them how they would explain the result to the patient. There was no association between answering both knowledge questions correctly and types of previous genetic tests ordered (p > .1 for 8 types of genetic tests), having received a genetic test report with a VUS result (p = .58), having referred patients to a genetics professional (p = .74), or feeling comfortable discussing a positive, negative, or VUS genetic test result (p > .4). This suggests that having previous experience ordering genetic tests does not contribute to the participants' knowledge about a variant of uncertain significance. Most participants reported that the amount of information in each section of the mock report was adequate. Participants were likely to reference multiple resources to better understand a VUS result, including published literature (82%), gene-specific databases (67%), and colleagues (63%). While these results cannot be generalized to all institutions, institutions can use the two knowledge questions to determine participants' understanding of genetic test results. This will help healthcare institutions determine methods that will best aide their providers who order genetic testing but do not specialize in genetics in learning more about the genetic testing process and better utilize results to improve patient care., (Copyright © 2021 National Society of Genetic Counselors.)
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- 2021
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16. Hypopigmentation in burns is associated with alterations in the architecture of the skin and the dendricity of the melanocytes.
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Dutta S, Panda S, Singh P, Tawde S, Mishra M, Andhale V, Athavale A, and Keswani SM
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- Adolescent, Adult, Burns complications, Burns metabolism, Cell Proliferation, Coculture Techniques, Female, Humans, Hypopigmentation etiology, Hypopigmentation metabolism, Immunohistochemistry, Keratin-5 metabolism, Keratinocytes metabolism, Ki-67 Antigen metabolism, Male, Melanins biosynthesis, Melanocytes metabolism, Membrane Proteins metabolism, Middle Aged, Primary Cell Culture, Receptor, Melanocortin, Type 1 metabolism, Trypsin metabolism, Young Adult, Burns pathology, Hypopigmentation pathology, Keratinocytes pathology, Melanins metabolism, Melanocytes pathology
- Abstract
Hypopigmentation is a major problem in deep dermal burns. To date, no standard treatment is available for the post burn hypopigmentation disorder. Therefore, understanding the molecular and cellular events are of benefit for therapeutic intervention. Hematoxylin and Eosin (H&E) and Fontana Masson (FM) staining of post burn hypopigmented skin (PBHS) showed an altered architectural pattern in cellular organization, cornified layer and melanin pigment as compared to the normal skin. This was confirmed by immunohistochemistry (IHC) analysis of PBHS samples using specific marker cytokeratin 5 (CK5) for keratinocytes and melanocortin 1 receptor (MCIR) for melanocytes. Validation of these observations was performed by IHC using proliferation and differentiation markers, Ki67 and Loricrin respectively and the melanocyte specific marker tyrosinase related protein 1 (TRP1). Taking a cue from the IHC study, the interaction of keratinocytes and melanocytes was studied by developing a co-culture model from PBHS and normal skin. Culture data exhibited a change of dendritic structure, reduced proliferation rate, faulty melanin synthesis and transfer of melanin from melanocytes to keratinocytes in PBHS samples. To the best of our knowledge, this is the first study showing structural and functional aberrations of melanocytes and keratinocytes, as a potential cause of hypopigmentation in burned patients. Our study, therefore, provides valuable insight for the basis of hypopigmentation in post burn patients, which may pave the way for clinical intervention in the future., (Copyright © 2019 Elsevier Ltd and ISBI. All rights reserved.)
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- 2020
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17. Reduction of Health Care Costs and Improved Appropriateness of Incoming Test Orders: the Impact of Genetic Counselor Review in an Academic Genetic Testing Laboratory.
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Wakefield E, Keller H, Mianzo H, Nagaraj CB, Tawde S, and Ulm E
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- Humans, Retrospective Studies, Counselors, Genetic Counseling, Genetic Testing economics, Genetic Testing methods, Health Care Costs, Laboratories
- Abstract
The goal of this study was to evaluate the impact of genetic counselor (GC) review of incoming test orders received in an academic diagnostic molecular genetics laboratory. The GC team measured the proportion of orders that could be modified to improve efficiency or sensitivity, tracked provider uptake of GC proposed testing changes, and calculated the health care dollar savings resulting from GC intervention. During this 6-month study, the GC team reviewed 2367 incoming test orders. Of these, 109 orders (4.6%) were flagged for review for potentially inefficient or inappropriate test ordering. These flagged orders corresponded to a total of 51 cases (1-5 orders for each patient), representing 54 individuals and including 3 sibling pairs. The GC team proposed a modification for each flagged case and the ordering providers approved the proposed change for 49 of 51 cases (96.08%). For the 49 modifications, the cost savings totaled $98,750.64, for an average of $2015.32 saved per modification. This study provides evidence of the significant contribution of genetic counselors in a laboratory setting and demonstrates the benefit of laboratories working with ordering providers to identify the best test for their patients. The review of test orders by a genetic counselor both improves genetic test ordering strategies and decreases the amount of health care dollars spent on genetic testing.
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- 2018
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18. Ex vivo Raman spectroscopic study of breast metastatic lesions in lungs in animal models.
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Bhattacharjee T, Tawde S, Hudlikar R, Mahimkar M, Maru G, Ingle A, and Murali Krishna C
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- Animals, Biomarkers, Tumor analysis, Cell Line, Tumor, Diagnosis, Computer-Assisted methods, In Vitro Techniques, Lung Neoplasms diagnosis, Mice, Inbred C3H, Reproducibility of Results, Sensitivity and Specificity, Breast Neoplasms chemistry, Lung Neoplasms chemistry, Lung Neoplasms secondary, Neoplasms, Experimental chemistry, Neoplasms, Experimental diagnosis, Spectrum Analysis, Raman methods
- Abstract
The lung is one of the most common sites of metastases, with approximately 50% of patients with extrathoracic cancer exhibiting pulmonary metastases. Correct identification of the metastatic status of a lung lesion is vital to therapeutic planning and better prognosis. However, currently available diagnostic techniques, such as conventional radiography and low dose computed tomography (LDCT), may fail to identify metastatic lesions. Alternative techniques such as Raman spectroscopy (RS) are hence being extensively explored for correct diagnosis of metastasis. The current ex vivo study aims to evaluate the ability of a fiber optic-based Raman system to distinguish breast cancer metastasis in lung from primary breast and lung tumor in animal models. In this study, spectra were acquired from normal breast, primary breast tumor, normal lung, primary lung tumor, and breast cancer metastasis in lung tissues and analyzed using principal component analysis and principal component-linear discriminant analysis. Breast cancer metastasis in lung could be classified with 71% classification efficiency. Approximately 6% breast metastasis spectra were misclassified with breast tumor, probably due to the presence of breast cancer cells in metastasized lungs. Test prediction results show 64% correct prediction of breast metastasis, while 13% breast metastasis spectra were wrongly predicted as breast tumor, suggesting the possible influence of breast cancer cells. Thus, findings of this study, the first of such explorations, demonstrate the potential of RS in classifying breast metastasis in lungs from primary lung and primary breast tumor. Prospective evaluation on a larger cohort with better multivariate analysis, combined with LDCT and recently developed real-time in vivo probes, RS can play a significant role in nonsurgical screening of lesions, which can lead to individualized therapeutic regimes and improved prognoses.
- Published
- 2015
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19. Characterization of influenza virus among influenza like illness cases in Mumbai, India.
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Roy S, Dahake R, Patil D, Tawde S, Mukherjee S, Athlekar S, Chowdhary A, and Deshmukh R
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The present study was carried out to monitor influenza viruses by identifying the virus and studying the seasonal variation during 2007-2009 in Mumbai. A total of 193 clinical respiratory samples (nasal and throat swab) were collected from patients having influenza like illness in Mumbai region. One-step real-time reverse-transcriptase PCR (rRTPCR) was used to detect Influenza type A (H1 and H3) and Influenza type B virus. Isolation of the virus was carried out using in vitro system which was further confirmed and typed by hemagglutination assay and hemagglutination inhibition assay. Out of 193 samples 24 (12.4 3%) samples tested positive for influenza virus, of which 13 (6.73 %) were influenza type A virus and 10 (5.18 %) were influenza type B virus, while 1 sample (0.51 %) was positive for both. By culture methods, 3 (1.55 %) viral isolates were obtained. All the three isolates were found to be Influenza type B/Malaysia (Victoria lineage) by Hemagglutination Inhibition Assay. The data generated from the present study reveals that both Influenza type A and B are prevalent in Mumbai with considerable activity. The peak activity was observed during monsoon season.
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- 2014
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20. Nanocrystalline titanium dioxide and magnesium oxide in vitro dermal absorption in human skin.
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van der Merwe D, Tawde S, Pickrell JA, and Erickson LE
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- Administration, Topical, Adult, Diffusion Chambers, Culture, Humans, In Vitro Techniques, Magnesium Oxide administration & dosage, Magnesium Oxide blood, Magnesium Oxide chemistry, Magnesium Oxide toxicity, Microscopy, Electron, Transmission, Models, Biological, Skin metabolism, Skin ultrastructure, Titanium administration & dosage, Titanium blood, Titanium chemistry, Titanium toxicity, Magnesium Oxide pharmacokinetics, Nanoparticles, Skin drug effects, Skin Absorption, Titanium pharmacokinetics
- Abstract
The dermal absorption potential of a nanocrystalline magnesium oxide (MgO) and titanium dioxide (TiO(2)) mixture in dermatomed human skin was assessed in vitro using Bronaugh-type flow-through diffusion cells. Nanocrystalline material was applied to the skin surface at a dose rate of 50 mg/cm(2) as a dry powder, as a water suspension, and as a water/surfactant (sodium lauryl sulfate) suspension, for 8 hours. Dermal absorption of nanocrystalline MgO and TiO(2) through human skin with intact, functional stratum corneum was not detectable under the conditions of this experiment.
- Published
- 2009
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21. Outcome of acute myeloid leukaemia in adults: a retrospective analysis.
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Saikia TK, Bakshi A, Bhagwat R, Tawde S, Nair R, Nair CN, and Parikh PM
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- Adolescent, Adult, Cytarabine administration & dosage, Daunorubicin administration & dosage, Female, Humans, Idarubicin administration & dosage, India, Male, Middle Aged, Prognosis, Remission Induction, Retrospective Studies, Survival Analysis, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Leukemia, Myeloid, Acute drug therapy
- Abstract
Background: There are little data from India on the management of acute myeloid leukaemia. With better understanding of the biology of the disease, and routine use of high-dose cytarabine as post-remission therapy with or without haematopoietic blood stem cell transplantation (HSCT), the results have improved in the past two decades. We analysed our results in a cohort of recently treated patients., Methods: A total of 166 newly diagnosed patients with AML (excluding acute promyelocytic leukaemia), 15-60 years of age were treated with daunorubicin (60 mg/m2/day x3 days) or idarubicin (12 mg/m2/day x3 days) with cytarabine (100 mg/m2/day continuous i.v. infusion x7 days) induction chemotherapy. Post-remission therapy included 2 cycles of high-dose cytarabine (15-18 g/m2) followed by monthly cycles of outpatient maintenance chemotherapy x4 cycles, consisting of daunorubicin (45 mg/m2 i.v. x1 day and cytarabine 100 mg/ m2 s.c. twice daily x5 days). Six patients in remission received sibling donor allogeneic HSCT., Results: Morphological complete remission was achieved in 69.9% of the patients. Resistant disease after induction chemotherapy was seen in 14.6% and early mortality occurred in 16%. Relapse-free survival and event-free survival at a median of 36 months was 34% and 22%, respectively. Relapse occurred in 43.9%. The median duration of remission was 12 months., Conclusions: Our results conform to the published literature from larger cooperative studies from the West. Currently available cytotoxic drugs are unlikely to improve the results any further.
- Published
- 2005
22. Allogeneic blood stem cell transplantation in chronic myeloid leukaemia-chronic phase following conditioning with busulphan and cyclophosphamide: a follow up report.
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Saikia TK, Parikh PM, Tawde S, Amare-Kadam PS, Rajadhyaksha S, and Chhaya S
- Subjects
- Adolescent, Adult, Busulfan therapeutic use, Child, Chronic Disease, Cyclophosphamide therapeutic use, Cyclosporine therapeutic use, Disease Progression, Disease-Free Survival, Female, Follow-Up Studies, Graft vs Host Disease prevention & control, HLA Antigens, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive immunology, Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology, Male, Neoplasm Recurrence, Local, Transplantation, Homologous adverse effects, Transplantation, Homologous immunology, Treatment Outcome, Hematopoietic Stem Cell Transplantation adverse effects, Immunosuppressive Agents therapeutic use, Leukemia, Myelogenous, Chronic, BCR-ABL Positive therapy, Transplantation Conditioning
- Abstract
Background: Allogeneic bone marrow transplantation (BMT) or peripheral blood stem cell transplantation remains the only modality of treatment that can eradicate a leukaemia clone in the majority of patients with chronic myeloid leukaemia (CML). However, the advent of the targeted molecule imatinib mesylate (formerly STI-571) against the bcr-abl chimeric protein in the disease has brought the issue of managing newly diagnosed CML patients, especially those with available donors, to the crossroads. Although the curative potential of this agent remains unknown, it can produce complete cytogenetic response in > 60% of newly diagnosed patients., Methods: From May 1991 to October 2002, a total of 55 Ph+ CML-chronic phase patients received oral busulphan 16 mg/kg and cyclophosphamide 120 mg/kg i.v. as a conditioning regimen. All patients received human leucocyte antigen (HLA)-identical sibling donor haematopoletic stem cells--bone marrow in 41 patients (74.5%) and peripheral blood stem cells in 14 (25.4%). Post-transplant prophylaxis for graft-versus-host disease included a short course of methotrexate (on days +1, +3, +6 and +11) and cyclosporin till day +180 in 38 patients (69.1%), while a combination of cyclosporin and methylprednisolone was used in the remaining 17 (29%)., Results: At a median follow up of 48 months (10-144 months), 26 patients (47.3%) are alive. Early mortality (100-day) occurred in 17 patients (30.9%). Acute graft-versus-host disease developed in 37 patients (67.3%), and was grade IV in 6 of them. Chronic graft-versus-host disease developed in 17 patients (30.9%). Relapse occurred in only 2 patients (3.6%) till date. The leukaemia-free survival is 64.3% in the peripheral stem cell group, whereas it is 41.5% in the bone marrow recipient group., Conclusion: Allogeneic BMT appears to result in eradication of CML and ensure disease-free survival in about half the patients. However, efforts should be made to prevent graft-versus-host disease and minimize early mortality.
- Published
- 2004
23. Physicochemical and immunochemical studies on the reaction of bovine serum albumin with p, p'-difluoro-m, m'-dinitrodiphenylsulfane.
- Author
-
TAWDE SS, RAM JS, and IYENGAR R
- Subjects
- Animals, Cattle, Serum Albumin, Serum Albumin, Bovine, Sulfones
- Published
- 1963
- Full Text
- View/download PDF
24. Studies in experimental hypercholesterolemia. III. State of plasma proteins and liver nucleic acids in rats fed extraneous cholesterol with different dietary fats.
- Author
-
CAMA HR, DAS BR, and TAWDE S
- Subjects
- Animals, Rats, Blood Proteins chemistry, Cholesterol blood, Dietary Fats, Hypercholesterolemia, Liver metabolism, Nucleic Acids metabolism
- Published
- 1961
25. Mechanisms of gamma-globulin synthesis.
- Author
-
Tawde S, Scharff MD, and Uhr JW
- Subjects
- Animals, Coliphages immunology, Dactinomycin pharmacology, HeLa Cells, In Vitro Techniques, Newcastle disease virus, RNA biosynthesis, RNA, Messenger pharmacology, Rabbits, Ribosomes metabolism, Lymphoid Tissue metabolism, gamma-Globulins biosynthesis
- Published
- 1966
26. Conjugation of antibody to ferritin by means of p,p'-difluoro-m, m'-dinitrodiphenylsulphone.
- Author
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TAWDE SS and RAM JS
- Subjects
- Antibodies chemistry, Ferritins chemistry, Sulfones chemistry
- Published
- 1962
- Full Text
- View/download PDF
27. Isolation and partial characterization of red gram (Cajanus cajan) trypsin inhibitor.
- Author
-
TAWDE S
- Subjects
- Cajanus, Trypsin Inhibitors, Vegetables chemistry
- Published
- 1961
28. Some investigations on experimental hypercholesterolemia in albino rats.
- Author
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CAMA HR, DAS BR, and TAWDE S
- Subjects
- Animals, Rats, Cholesterol blood, Hypercholesterolemia
- Published
- 1961
29. Preparation of antibody-ferritin conjugates for immunoelectron microscopy.
- Author
-
SRI RAM J, TAWDE SS, PIERCE GB Jr, and MIDGLEY AR Jr
- Subjects
- Antibodies, Antigens, Ferritins, Immunoglobulins, Microscopy, Immunoelectron
- Published
- 1963
- Full Text
- View/download PDF
30. Studies in experimental hypercholesterolemia in rats. IV. Effect of dietary cholesterol on plasma and hepatic proteins of adult rats maintained on high fat diets.
- Author
-
TAWDE S and DAS BR
- Subjects
- Animals, Rats, Blood Proteins chemistry, Cholesterol, Cholesterol, Dietary, Diet, Diet, High-Fat, Fats, Hypercholesterolemia, Liver chemistry, Nutrition Assessment, Proteins chemistry
- Published
- 1962
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