109 results on '"Takashi, Kusunoki"'
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2. Atopic dermatitis and its risk/aggravation factors -a special focus on food
- Author
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Takashi Kusunoki
- Subjects
General Medicine - Published
- 2023
3. Management of Food Allergy in Nurseries in Japan: a comparison between 2013 and 2021
- Author
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Yuzuna Tanaka, Ayu Kawabata, Yuie Motoyama, and Takashi Kusunoki
- Subjects
General Medicine - Published
- 2023
4. Collision-free formation control of two-wheeled vehicles based on triangular LQ optimal regulator.
- Author
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Takashi Kusunoki, Daisuke Tsubakino, and Yuh Yamashita
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- 2014
- Full Text
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5. Association of Skin Carotenoid Score and Food Intake among School Children: A Multicenter Cross-Sectional Study
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Jiro TAKEUCHI, Takashi KUSUNOKI, and Takeshi MORIMOTO
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Male ,Eating ,Nutrition and Dietetics ,Cross-Sectional Studies ,Vegetables ,Medicine (miscellaneous) ,Humans ,Female ,Child ,Carotenoids ,Diet - Abstract
This study aimed to evaluate the associations between skin carotenoid measurements and fruit and vegetables intake among school children. We conducted a cross-sectional study for 10-y-old children in 2020 in Japan. We compared the optical skin carotenoid score, measured using Veggie Meter
- Published
- 2022
6. Role of doctors and other medical professions in the care of patients with allergies
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Takashi Kusunoki
- Subjects
medicine.medical_specialty ,Allergy ,business.industry ,Family medicine ,Medicine ,business ,medicine.disease - Published
- 2021
7. Fall birth is associated with prolonged elimination in Japanese children with hen's egg allergy: A population-based study
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Takeshi Morimoto, Takashi Kusunoki, Yuie Motoyama, and Jiro Takeuchi
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Male ,Pediatrics ,medicine.medical_specialty ,Adolescent ,MEDLINE ,Japan ,Odds Ratio ,medicine ,Humans ,Immunology and Allergy ,Child ,Egg Hypersensitivity ,business.industry ,Parturition ,General Medicine ,RC581-607 ,medicine.disease ,Population based study ,Child, Preschool ,Population Surveillance ,Egg allergy ,Female ,Seasons ,Immunologic diseases. Allergy ,business ,Food Hypersensitivity - Published
- 2021
8. A parallel reinforcement computing model for function optimization problems.
- Author
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Qian Fei, Shigeya Ikebou, Takashi Kusunoki, Jijun Wu, and Hironori Hirata
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- 2000
- Full Text
- View/download PDF
9. Helicobacter cinaedi-Associated Refractory Cellulitis in Patients with X-Linked Agammaglobulinemia
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Tomohiro Morio, Hirokazu Kanegane, Kento Inoue, Takahiro Yasumi, Takashi Kusunoki, Saeko Sasaki, and Kohsuke Imai
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Immunology ,X-linked agammaglobulinemia ,Bacteremia ,medicine.disease_cause ,Helicobacter Infections ,Haemophilus influenzae ,Hypogammaglobulinemia ,Helicobacter cinaedi ,Agammaglobulinemia ,Helicobacter ,hemic and lymphatic diseases ,medicine ,Humans ,Immunology and Allergy ,Blood culture ,biology ,medicine.diagnostic_test ,business.industry ,Disease Management ,Cellulitis ,Genetic Diseases, X-Linked ,biology.organism_classification ,medicine.disease ,Disease Susceptibility ,business - Abstract
X-linked agammaglobulinemia (XLA) is characterized by severe or recurrent infections, hypogammaglobulinemia, and circulating B cell deficiency. The frequent pathogens seen in patients with XLA include Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, and enterovirus as well as Campylobacter and Helicobacter species. Here, we describe two patients with XLA who developed cellulitis and bacteremia caused by Helicobacter cinaedi even when administered an appropriate immunoglobulin replacement therapy. H. cinaedi may be difficult to isolate using a conventional blood culture system and could be identified by sequence analysis and mass spectrometry. H. cinaedi infection causes recurrent symptoms frequently, and patients require a long course of antibiotic treatment. Recently, the case of non-H. pylori Helicobacter (NHPH) infection such as H. cinaedi and H. bilis infection is increasing in number in patients with XLA. Systemic NHPH infection should be suspected, and extensive microbiological analysis should be performed to appropriately treat patients with XLA who present with fever and skin lesions.
- Published
- 2020
10. The Effect of Past Food Avoidance Due to Allergic Symptoms on the Growth of Children at School Age
- Author
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Kumiko Mukaida, Takashi Kusunoki, Takeshi Morimoto, Takahiro Yasumi, Ryuta Nishikomori, Toshio Heike, Tatsuya Fujii, and Tatsutoshi Nakahata
- Subjects
body mass index ,epidemiology ,food avoidance ,growth ,schoolchildren ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: The influence of food avoidance due to allergic symptoms in infancy on the growth of children at school age has not been well evaluated. Methods: To determine the growth of schoolchildren who avoided eggs, milk, or wheat due to immediate allergic symptoms in infancy (food avoiders in infancy) (FAI), a questionnaire on the presence of allergic diseases, as well as present height and weight, was administered to the parents of 14,669 schoolchildren. 11,473 subjects had available data. The height and weight standard deviation scores (HtSDS and WtSDS) and body mass index percentile (BMI percentile) of each subject were calculated. Results: FAI had significantly lower WtSDS than non-FAI (P = 0.01). Among those with avoidance at age 3 years, those who avoided two or more foods and those who avoided milk had significantly lower HtSDS than their counterparts (P = 0.02 and 0.04, respectively). FAI had a significantly lower prevalence of obesity (P = 0.01) and overweight (P = 0.002), while there was no difference in the prevalence of underweight (P = 0.58), resulting in a significantly higher prevalence of appropriate weight (P = 0.01) compared to non-FAI. Significantly lower prevalence of obesity and overweight was observed even among those who terminated the avoidance by age 3 years. Conclusions: FAI were less likely to be obese or overweight, resulting in a higher prevalence of appropriate weight at school age. Further investigation should contribute to better management of food allergy and obesity.
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- 2010
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11. Changing Prevalence and Severity of Childhood Allergic Diseases in Kyoto, Japan, from 1996 to 2006
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Takashi Kusunoki, Takeshi Morimoto, Ryuta Nishikomori, Takahiro Yasumi, Toshio Heike, Tatsuya Fujii, and Tatsutoshi Nakahata
- Subjects
allergic disease ,epidemiology ,prevalence ,questionnaire ,schoolchildren ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: Published data regarding changes in the prevalence of childhood allergic diseases in Japan have been limited. Methods: To observe changes in the recent trends of the childhood allergy epidemic in Japan, a population- based questionnaire survey of allergic diseases was conducted among 13,215 schoolchildren, aged 7 to 15 years, in Kyoto, Japan in 2006. The results were compared with those obtained in the 1996 survey using the same scale and methods in the same region. Results: The prevalences of bronchial asthma (BA), atopic dermatitis (AD), allergic rhinitis (AR), and allergic conjunctivitis (AC) in 1996 and 2006 were 5.1% and 5.0% (p = 0.58), 4.2% and 5.6% (p < 0.0001), 20.3% and 27.4% (p < 0.0001), and 13.3% and 25.2% (p < 0.0001), respectively. Although the distribution of BA severity improved, the severity distribution of AD, AR, and AC all deteriorated. The lifetime prevalence (present prevalence and past history combined) of BA increased from 6.5% to 7.6% (p < 0.0001). The sex ratio analysis showed that the female predominance in the prevalence of AD observed in 1996 disappeared in 2006, indicating a particular rise in AD prevalence among boys. Conclusions: Overall, the results indicate that the rising trend of allergic diseases, especially in AD, AR, and AC, continues among schoolchildren living in Kyoto, Japan. Special attention should be paid to skin and naso- ocular symptoms.
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- 2009
- Full Text
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12. Risk Factors for Cerebral Infarction in Duchenne Muscular Dystrophy: Review With our 2 Cases
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Minoru Shibata, Takashi Kusunoki, Fumihito Nozaki, Tomohiro Kumada, and Tatsuya Fujii
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Adult ,Cardiomyopathy, Dilated ,Male ,medicine.medical_specialty ,Adolescent ,Health Status ,Duchenne muscular dystrophy ,Population ,Infarction ,Risk Assessment ,Ventricular Function, Left ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Mobility Limitation ,Risk factor ,Child ,education ,education.field_of_study ,Ejection fraction ,business.industry ,Cerebral infarction ,Rehabilitation ,Age Factors ,Stroke Volume ,Dilated cardiomyopathy ,Atrial fibrillation ,Cerebral Infarction ,Prognosis ,medicine.disease ,Muscular Dystrophy, Duchenne ,Diffusion Magnetic Resonance Imaging ,Child, Preschool ,cardiovascular system ,Cardiology ,Surgery ,Neurology (clinical) ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
Background: Although the incidence of cerebral infarction is higher in Duchenne muscular dystrophy (.75 per 100) than in the general population (7.5-11.4 per 100 000), only 18 cases have been reported, and prevention and management guidelines for infarction in this disorder remain lacking. Patients and Methods: We encountered 2 cases of Duchenne muscular dystrophy with cerebral infarction. To clarify risk factors for such infarction in Duchenne muscular dystrophy, we reviewed 20 cases, including our 2 patients. Results: Age at onset of infarction ranged from 4 to 31 years (n = 19). Most patients were 16-21 years old (14 of 19; 73.7%). Eighteen patients (90%) had dilated cardiomyopathy (DCM), showing a higher frequency than in the age-matched general Duchenne muscular dystrophy population. Left ventricular ejection fraction (LVEF) ranged from 10.2% to 42% (median, 20%; n = 9). Detectable cardiac thrombus and atrial fibrillation were rare (2 of 17; 11.8%, and 1 of 17; 5.9%, respectively). Conclusions: Presence of DCM with low LVEF seems to be the strongest risk factor for cerebral infarction in Duchenne muscular dystrophy.
- Published
- 2019
13. Negative Association in Reported Dietary Energy Intake and Physique in Japanese Schoolchildren
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Takashi Kusunoki, Mihoko Kojima, Hirokazu Taniguchi, Taro Suzuki, Kengo Yoshii, Akane Higashi, and Kiyomi Harada
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0301 basic medicine ,Male ,Percentile ,Longitudinal study ,Pediatric Obesity ,Energy (esotericism) ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,Negative association ,Energy requirement ,Body Mass Index ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Environmental health ,Medicine ,Humans ,Longitudinal Studies ,Child ,030109 nutrition & dietetics ,Nutrition and Dietetics ,business.industry ,Diet ,Cross-Sectional Studies ,Negative relationship ,Health education ,Female ,business ,Energy Intake ,Body mass index - Abstract
Appropriate dietary assessment and health education are necessary for children to achieve a healthy physique. To explore the relationship between habitual reported dietary energy intake (EI) and physique in elementary schoolchildren by sex and age, we conducted a longitudinal study, in the fiscal year 2011, that included all elementary schools in Omihachiman City, Shiga Prefecture, Japan. The study lasted for four consecutive years, ending in fiscal year 2014, and included 545 7-y-old schoolchildren in the target city. The subjects completed a brief self-administered diet history questionnaire with their guardians. The results of the study demonstrated a negative relationship between energy intake and the estimated energy requirement ratio and body mass index percentile values for both 7-, 9-, and 10-y-old boys and 7- to 10-y-old girls. These results suggest that there is a need to keep in consideration the under-reporting of obese children and over-reporting of lean children for dietary energy evaluation.
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- 2021
14. Serum levels of soluble CD14 in allergic inflammation
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Takashi Kusunoki, Samuel D. Wright, Yasuhiro Inoue, Takeshi Miyanomae, Yoko Yoshida, and Kozo Yoneda
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asthma ,atopic dermatitis ,CD14 ,inflammation ,macrophage ,monocyte ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Monocytes/macrophages have recently been shown to play a significant role in the pathogenesis of allergic diseases. As the level of soluble CD14 (sCD14) in serum is considered a marker of monocyte/macrophage activation, we measured the levels of sCD14 in allergic asthma and atopic dermatitis (AD), along with acute infectious and inflammatory diseases, to see its clinical relevance. Serum samples were taken from patients with acute infectious and inflammatory diseases, allergic asthma, and atopic dermatitis. sCD14 was measured with our own ELISA system and its level in each disease was compared with normal controls as well as its disease severity. sCD14 was elevated and correlated with C-reactive protein in infectious and inflammatory diseases (n = 26), confirming that it reflects inflammation. sCD14 was also significantly increased both in asthma (n = 94) and adult chronic AD (n = 22). In asthmatic patients, those with higher sCD14 tended to have more severe symptoms, but there was no statistical correlation between sCD14 and severity. In adult chronic AD patients, a correlation between sCD14 and disease severity was observed. However, sCD14 was not elevated in infant AD patients (n = 18) irrespective of severity, suggesting differences in the degree of monocyte/macrophage involvement in the pathogenesis between adult chronic and infant AD. The levels of sCD14 were shown to be upregulated in allergic diseases and might be useful as a marker of monocyte/macrophage involvement in allergic inflammation.
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- 1998
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15. Slow stepwise resolution protocol for children allergic to hen’s egg, milk or wheat
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Jiro Takeuchi, Takashi Kusunoki, Fumihito Nozaki, Kenji Inoue, and Kumiko Mukaida
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03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,030228 respiratory system ,business.industry ,Family medicine ,medicine ,030212 general & internal medicine ,General Medicine ,business - Published
- 2018
16. Asthma death in Japanese children committee report in 2017
- Author
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Takanari Imai, Mizuho Nagao, Yukihiro Ohya, Hironobu Fukuda, Masaki Futamura, Takehiko Matsui, Toshiko Itazawa, Hiroshi Odajima, Akira Akasawa, Takashi Kusunoki, Sankei Nishima, Koichi Arakawa, and Koichi Yoshida
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medicine.medical_specialty ,business.industry ,Committee report ,Family medicine ,medicine ,medicine.disease ,business ,Asthma - Published
- 2018
17. Left ventricular noncompaction cardiomyopathy in a patient with trisomy 13: A report and review of the literature
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Anri Hayashi, Minoru Shibata, Tatsuya Fujii, Fumihito Nozaki, Takashi Kusunoki, Ikuko Hiejima, and Tomohiro Kumada
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medicine.medical_specialty ,Heart disease ,Heart malformation ,business.industry ,030204 cardiovascular system & hematology ,medicine.disease ,Left ventricular noncompaction cardiomyopathy ,Sudden death ,Asymptomatic ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Heart failure ,Genetics ,Cardiology ,medicine ,medicine.symptom ,Trisomy ,business ,Stroke ,030217 neurology & neurosurgery ,Genetics (clinical) - Abstract
Left ventricular noncompaction cardiomyopathy (LVNC) is characterized by prominent trabecular meshwork, and it is thought to result from arrest of the normal compaction process during embryogenesis. Patients with LVNC may be asymptomatic or have symptoms ranging from heart failure to stroke, life-threatening arrhythmias, or sudden death. The frequency of LVNC in children has increased with longer clinical courses. About 80% of patients with trisomy 13 have a congenital cardiac abnormality, but a clinical description of LVNC with trisomy 13 is lacking because of its poor prognosis and lack of awareness about LVNC. We described a patient with trisomy 13 who was diagnosed with LVNC-dilated phenotype and died suddenly, as well as two additional patients with LVNC. All three patients had chronic heart failure without congenital heart disease and were treated with diuretics. To manage trisomy 13 with or without congenital heart disease, cardiac disease such as LVNC may present at any ages, and therefore cardiac evaluation should be considered as a part of their appropriate management.
- Published
- 2017
18. Correction to: Helicobacter cinaedi-Associated Refractory Cellulitis in Patients with X-Linked Agammaglobulinemia
- Author
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Kohsuke Imai, Takashi Kusunoki, Tomohiro Morio, Hirokazu Kanegane, Kento Inoue, Takahiro Yasumi, and Saeko Sasaki
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medicine.medical_specialty ,biology ,business.industry ,Immunology ,MEDLINE ,X-linked agammaglobulinemia ,medicine.disease ,biology.organism_classification ,Dermatology ,Helicobacter cinaedi ,Medical microbiology ,Refractory ,Cellulitis ,Immunology and Allergy ,Medicine ,In patient ,business - Published
- 2021
19. ALDH18A1-related cutis laxa syndrome with cyclic vomiting
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Yuto Yamamoto, Tatsuhiko Tsunoda, Nobuhiko Okamoto, Tatsuya Fujii, Takashi Kusunoki, Kenjiro Kosaki, Tomohiro Kumada, Minoru Shibata, Fumihito Nozaki, and Fuyuki Miya
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Male ,0301 basic medicine ,medicine.medical_specialty ,Microcephaly ,Foot Deformities, Congenital ,Arginine ,Vomiting ,030105 genetics & heredity ,Short stature ,Cutis Laxa ,Diagnosis, Differential ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Developmental Neuroscience ,Internal medicine ,medicine ,Citrulline ,Humans ,Child ,business.industry ,Brain ,Syndrome ,General Medicine ,Aldehyde Dehydrogenase ,Ornithine ,medicine.disease ,Hypotonia ,Endocrinology ,chemistry ,Face ,Pediatrics, Perinatology and Child Health ,Neurology (clinical) ,medicine.symptom ,business ,Hand Deformities, Congenital ,Blood Chemical Analysis ,030217 neurology & neurosurgery ,Cutis laxa - Abstract
Cutis laxa (CL) syndromes are connective tissue disorders characterized by redundant, sagging, inelastic and wrinkled skin, with organ involvement. Here, we describe a patient with ALDH18A1-related CL who developed cyclic vomiting. The patient was a 12-year-old boy who presented with poor postnatal growth, hypotonia, short stature, joint hyperlaxity, microcephaly, strabismus, bilateral cataracts, facial dysmorphism and severe mental retardation. Bone radiographs showed osteopenia and osteoporosis, and magnetic resonance angiography showed marked kinking and tortuosity of the brain vessels. These findings were clinically compatible with ALDH18A1-related CL. Molecular analysis revealed a de novo heterozygous mutation (p.R138Q) in ALDH18A1. No mutations were found in PYCR1 gene. The patient developed cyclic vomiting with decreased blood levels of ornithine, citrulline, arginine and proline without hyperammonemia and other hypoaminoacidemias were also found. ALDH18A1 encodes Δ(1)-pyrroline-5-carboxylate synthase, which is related to the biosynthesis of ornithine, citrulline, arginine, and proline. Cyclic vomiting has never been reported in other ALDH18A1-related CL patients. This is the first case report of ALDH18A1-related CL with cyclic vomiting associated with amino acid abnormalities.
- Published
- 2016
20. Secondary biotin, carnitine and selenium deficiency caused by long-term use of casein hydrolyzed formula
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Fumihito Nozaki and Takashi Kusunoki
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medicine.medical_specialty ,business.industry ,Family medicine ,Medicine ,business - Published
- 2016
21. Management of food-induced anaphylaxis and ownership of adrenaline autoinjectors among children in nurseries
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Kazuo Nonomura, Akio Kihara, Takashi Kusunoki, Naruo Saito, Seiro Narumiya, Tsuyoshi Ishigami, Yoshitaka Iwai, Tsuneo Hirota, and Yasuyuki Nomura
- Subjects
03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,030228 respiratory system ,business.industry ,Food induced anaphylaxis ,Anesthesia ,Emergency medicine ,medicine ,030212 general & internal medicine ,business - Published
- 2016
22. Severe acute abdomen caused by symptomatic Meckel's diverticulum in three children with trisomy 18
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Anri Hayashi, Takashi Kusunoki, Oki Furukawa, Minoru Shibata, Fumihito Nozaki, Ikuko Hiejima, Tatsuya Fujii, and Tomohiro Kumada
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Pediatrics ,medicine.medical_specialty ,Gastrointestinal bleeding ,Trisomy ,Intestinal Volvulus ,Intussusception (medical disorder) ,Genetics ,medicine ,Humans ,Genetics (clinical) ,Abdomen, Acute ,Meckel's diverticulum ,Gastrointestinal tract ,business.industry ,Infant, Newborn ,Infant ,medicine.disease ,Meckel Diverticulum ,Acute abdomen ,Child, Preschool ,Female ,medicine.symptom ,Chromosomes, Human, Pair 18 ,Gastrointestinal Hemorrhage ,business ,Intussusception ,Trisomy 18 Syndrome ,Diverticulum - Abstract
Meckel's diverticulum (MD) is the most prevalent congenital anomaly of the gastrointestinal tract and often presents a diagnostic challenge. Patients with trisomy 18 frequently have MD, but the poor prognosis and lack of consensus regarding management for neonates has meant that precise information on the clinical manifestations in infants and children with MD is lacking. We describe the cases of three children with trisomy 18 who developed symptomatic MD. Intussusception was diagnosed in Patient 1, intestinal volvulus in Patient 2, and gastrointestinal bleeding in Patient 3. All three patients underwent surgical treatment and only the Patient 1 died due to pulmonary hypertensive crisis. The other two patients experienced no further episodes of abdominal symptoms. In patients with trisomy 18, although consideration of postoperative complications and prognosis after surgical treatment is necessary, symptomatic MD should carry a high index of suspicion in patients presenting with acute abdomen.
- Published
- 2015
23. Asthma death in Japanese children committee report in 2014
- Author
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Hiroshi Odajima, Hirokazu Arakawa, Takashi Kusunoki, Shinichi Sumimoto, Koichi Yamaguchi, Takehiko Matsui, and Sankei Nishima
- Published
- 2015
24. Fruit intake reduces the onset of respiratory allergic symptoms in schoolchildren
- Author
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Mio Sakuma, Takeshi Morimoto, Takashi Kusunoki, Takahiro Yasumi, Ryuta Nishikomori, Akane Higashi, Jiro Takeuchi, and Toshio Heike
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Ragweed ,Male ,Pediatrics ,medicine.medical_specialty ,Immunology ,Diet Surveys ,Food group ,Allergic sensitization ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Internal medicine ,Epidemiology ,medicine ,Odds Ratio ,Prevalence ,Respiratory Hypersensitivity ,Immunology and Allergy ,Humans ,030212 general & internal medicine ,Longitudinal Studies ,Prospective Studies ,Prospective cohort study ,Child ,Asthma ,biology ,business.industry ,Odds ratio ,Protective Factors ,biology.organism_classification ,medicine.disease ,Confidence interval ,Diet ,Logistic Models ,030228 respiratory system ,Fruit ,Pediatrics, Perinatology and Child Health ,Female ,business - Abstract
Background Previous studies have shown that dietary pattern is associated with allergy prevention. Methods We conducted a prospective cohort study on all primary schools in Omihachiman City, Shiga Prefecture, Japan. Questionnaires regarding allergic symptoms and diet were distributed to the parents of all 759 7-year-old schoolchildren for 4 consecutive years, from 2011–2014. Specific immunoglobulin E to inhalant allergens were measured at 10 years of age. Participants were then categorized as low, medium, or high intake during the study period for four food groups (fruits, vegetables, fish and beans). Logistic regression analysis was performed to estimate odds ratios and 95% confidence intervals. Results A total of 520 children (68.5%) whose parents responded to the questionnaires all 4 years were included in the analysis. The prevalence of asthma, rhinitis, and any allergic symptoms at age 10 was significantly decreased with increases in fruit intake. In addition, the onset of any allergic symptoms during the study period was significantly decreased with increases in fruit intake (33.3%, 28.3%, and 14.3% in children with low, medium, and high fruit intake, respectively; P for trend = 0.01). The sensitization rate to ragweed at age 10 was significantly decreased with increases in fruit intake (P for trend = 0.046). No significant effect was observed for the other three food groups, except for the association between fish intake and new onset asthma symptoms. Conclusions These findings suggest that higher intake of fruit can help prevent respiratory allergic symptoms in schoolchildren. This article is protected by copyright. All rights reserved.
- Published
- 2017
25. OBESITY AND ATOPIC DERMATITIS, ALLERGIC RHINITIS
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Takashi, Kusunoki
- Subjects
Body Weight ,Humans ,Obesity ,Exercise ,Rhinitis, Allergic ,Dermatitis, Atopic - Published
- 2017
26. Organizing pneumonia as an extraintestinal manifestation of Crohn's disease in a child
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Takashi Kusunoki, Tatsuya Fujii, and Kenji Inoue
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Pulmonary and Respiratory Medicine ,Male ,medicine.medical_specialty ,Adolescent ,medicine.drug_class ,Antibiotics ,Disease ,03 medical and health sciences ,0302 clinical medicine ,Crohn Disease ,Internal medicine ,Biopsy ,medicine ,Humans ,Crohn's disease ,Gastrointestinal tract ,medicine.diagnostic_test ,business.industry ,Interstitial lung disease ,Pneumonia ,medicine.disease ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,Immunology ,Etiology ,030211 gastroenterology & hepatology ,business ,Tomography, X-Ray Computed - Abstract
Crohn's disease (CD) is a chronic inflammatory disorder with an unknown etiology that commonly involves the gastrointestinal tract, and bronchopulmonary manifestations only occur in 0.4% of cases. There have not been any reports about pulmonary involvement in pediatric CD patients. We experienced a 14-year-old boy with Crohn's disease diagnosed with organizing pneumonia by chest CT-guided biopsy examination. His pneumonia was intractable despite the administration of multiple antibiotics, and steroid therapy was very effective. In pediatric patients with CD whose lung disease does not respond to antibiotics, OP should be considered as a possible diagnosis.
- Published
- 2017
27. Slow stepwise resolution protocol for children allergic to egg, milk, or wheat: comparison between schoolchildren and preschool children
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Kenji Inoue, Takashi Kusunoki, Kumiko Mukaida, Fumihito Nozaki, and Jiro Takeuchi
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Protocol (science) ,Pediatrics ,medicine.medical_specialty ,business.industry ,Immunology ,Resolution (electron density) ,Immunology and Allergy ,Medicine ,business - Published
- 2019
28. Reversible Splenic Lesion in a Patient with Febrile Infection-Related Epilepsy Syndrome (FIRES)
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Tomoko Miyajima, Tomohiro Kumada, Ikuko Hiejima, Fumihito Nozaki, Takashi Kusunoki, Tatsuya Fujii, and Anri Hayashi
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Male ,Fever ,Encephalopathy ,Splenium ,Status epilepticus ,Corpus Callosum ,Diagnosis, Differential ,Lesion ,Humans ,Medicine ,Child ,Epilepsy ,business.industry ,Syndrome ,General Medicine ,medicine.disease ,Magnetic Resonance Imaging ,Febrile infection related epilepsy syndrome ,Treatment Outcome ,nervous system ,Anesthesia ,Pediatrics, Perinatology and Child Health ,Epilepsy syndromes ,Encephalitis ,Neurology (clinical) ,medicine.symptom ,business ,Splenial - Abstract
Febrile infection-related epilepsy syndrome (FIRES) is a severe epileptic syndrome that manifests with refractory seizures or status epilepticus in previously healthy children after banal febrile illness. The neuroimaging findings in the acute phase of FIRES are nonspecific or normal. We report the case of a 7-year-old boy with FIRES who presented with a reversible lesion in the splenium of the corpus callosum on brain magnetic resonance imaging (MRI). The patient developed clusters of clonic seizures with a deviation of the eyes after a 3-day history of fever. A reversible splenial lesion was observed on brain MRI and, therefore, the initial diagnosis was mild encephalitis/encephalopathy with a reversible splenial lesion (MERS). However, the intractable complex partial seizures necessitated a long-term midazolam infusion, indicating that FIRES was a more likely diagnosis than MERS. All other findings of this patient met the diagnostic criteria for FIRES. With this diagnosis, a high-dose phenobarbital was administrated, and the seizures were successfully controlled. This case indicated that FIRES should be considered even in patients with a reversible splenial lesion associated with encephalitis/encephalopathy.
- Published
- 2013
29. Effect of eczema on the association between season of birth and food allergy in Japanese children
- Author
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Takashi Kusunoki, Kumiko Mukaida, Toshio Heike, Takeshi Morimoto, Takahiro Yasumi, Ryuta Nishikomori, and Mio Sakuma
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Pediatrics ,medicine.medical_specialty ,Multivariate analysis ,Season of birth ,business.industry ,Confounding ,Retrospective cohort study ,Economic shortage ,Logistic regression ,medicine.disease ,Food allergy ,Pediatrics, Perinatology and Child Health ,Vitamin D and neurology ,Medicine ,business - Abstract
Background Food allergy (FA) in childhood has been shown to be more prevalent in those born in autumn and winter. The mechanisms of this season-of-birth effect remain unclear, although shortage of vitamin D during infancy has been considered one possible mechanism. The purpose of this study was to investigate the effect of eczema on the season-of-birth effect on FA in infancy. Methods A questionnaire survey on the prevalence of allergic diseases was completed by the parents of 14 669 Japanese schoolchildren, aged 7–15 years, in Kyoto City, Japan. Logistic regression models were constructed to compare the prevalence of FA in infancy according to season of birth. Results Those born in autumn and winter had a significantly higher prevalence of FA in infancy compared to those born in spring and summer in a multivariate model (4.8% vs 3.6%, P = 0.001). The difference, however, was no longer significant when eczema before 6 months was included as either an additional or only confounding factor. The difference among those with and without eczema before 6 months was further analyzed, and it was found that, in both groups, there was no difference between those born in spring and summer and those born in autumn and winter. Conclusions The season-of-birth effect on FA in infancy was significantly affected by the existence of eczema before 6 months in Japanese children. Eczema before 6 months may be the factor directly related to the season-of-birth effect on FA in infancy.
- Published
- 2012
30. Association of age and family history with supplement use in pediatric patients with allergy
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Shoko Hamada, Takashi Kusunoki, Ayako Nagai, Takahiro Kiyomasu, Masaru Kubota, Yutaka Suehiro, Yoshihiro Wakazono, Shiro Seto, and Nagisa Mori
- Subjects
Male ,Parents ,Pediatrics ,medicine.medical_specialty ,Allergy ,Adolescent ,Cross-sectional study ,Endocrinology, Diabetes and Metabolism ,Chlorella ,Aquatic organisms ,Endocrinology ,Japan ,Surveys and Questionnaires ,Supplement use ,Epidemiology ,Hypersensitivity ,Humans ,Medicine ,Family ,Micronutrients ,Family history ,Child ,Dosage Forms ,Biological Products ,Minerals ,Univariate analysis ,Nutrition and Dietetics ,business.industry ,Probiotics ,Nutritional content ,Age Factors ,Infant ,Vitamins ,medicine.disease ,Cross-Sectional Studies ,Case-Control Studies ,Child, Preschool ,Dietary Supplements ,Female ,Nutrition Therapy ,business - Abstract
This study was conducted to determine the frequency and characteristics of supplement use in pediatric patients with allergic disorders in Japan. A total of 229 patients with various allergic disorders aged between 0 and 15 years were enrolled. Supplements were defined as preparations that provided nutritional content in the form of a tablet, capsule, powder, liquid, or jelly. The parents of each subject were asked to complete a questionnaire on their child's use of supplements over the previous year. Demographic information, parents' perceived view of the child's health status over the previous month, and family history of both allergic disorders, and supplement use were collected. Four hundred eight age- and sex-matched healthy children served as the controls. Twenty-nine (12.7%) patients had used supplements. This frequency was not significantly different from that in the control group (15.0%). The types of supplements most commonly used were vitamins, followed by minerals, probiotics, and chlorella. Univariate analysis revealed that older age and a positive family history of supplement use were associated with patients' supplement use. The types of allergic disorders, health status from the point of view of the parents, and a family history of allergic disorders did not show any significant association. To our knowledge, this is the first cross-sectional study to demonstrate the frequency and the factors affecting supplement use in pediatric patients with allergic disorders.
- Published
- 2012
31. Birth order effect on childhood food allergy
- Author
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Kumiko Mukaida, Takahiro Yasumi, Ryuta Nishikomori, Toshio Heike, Takeshi Morimoto, Takashi Kusunoki, and Mio Sakuma
- Subjects
Allergy ,Pediatrics ,medicine.medical_specialty ,business.industry ,Immunology ,Atopic dermatitis ,medicine.disease ,Allergic conjunctivitis ,Birth order ,Food allergy ,Wheeze ,Pediatrics, Perinatology and Child Health ,Epidemiology ,medicine ,Immunology and Allergy ,medicine.symptom ,business ,Asthma - Abstract
To cite this article: Kusunoki T, Mukaida K, Morimoto T, Sakuma M, Yasumi T, Nishikomori R, Heike T. Birth order effect on childhood food allergy. Pediatric Allergy Immunology 2012: 23: 250–254. Abstract Higher birth order is associated with a smaller risk of allergy (birth order effect). The purpose of this study was to compare the significance of the birth order effect on the prevalence of specific allergic diseases [bronchial asthma (BA), atopic dermatitis (AD), allergic rhinitis (AR), allergic conjunctivitis (AC), and food allergy (FA)] among schoolchildren. A questionnaire survey dealing with the prevalence of allergic diseases was administered to the parents of 14,669 schoolchildren aged 7–15 yr. Based on the data, the prevalence of each allergic disease was compared according to birth order (1st, 2nd, and 3rd or later). Multiple regression analysis was performed to test the significance of the differences. There was no significant difference in the prevalence of BA or AD according to birth order. The prevalence of AR, AC, and FA decreased significantly as birth order increased. The prevalence of FA among those with 1st, 2nd, and 3rd or later birth order was 4.0%, 3.4%, and 2.6%, respectively (p = 0.01). With respect to symptoms in infancy, the prevalence of wheeze increased significantly and that of FA and eczema in infancy decreased significantly as birth order increased. The present data show a significant birth order effect on FA. The effect was also observed for the prevalence of FA and eczema in infancy. These data support the concept of early, non-allergen-specific programming of IgE-mediated immunity.
- Published
- 2012
32. Sports activities enhance the prevalence of rhinitis symptoms in schoolchildren
- Author
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Takashi Kusunoki, Takahiro Yasumi, Ryuta Nishikomori, Mio Sakuma, Takeshi Morimoto, Kumiko Mukaida, Toshio Heike, and Jiro Takeuchi
- Subjects
Intoxicative inhalant ,Male ,medicine.medical_specialty ,Pediatrics ,Allergy ,Immunology ,Population ,Prevalence ,Immunoglobulin E ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Environmental health ,Epidemiology ,Immunology and Allergy ,Medicine ,Humans ,030212 general & internal medicine ,Sports activity ,education ,Child ,Asthma ,education.field_of_study ,biology ,business.industry ,medicine.disease ,Rhinitis, Allergic ,030228 respiratory system ,Pediatrics, Perinatology and Child Health ,biology.protein ,Female ,business ,human activities ,Sports - Abstract
Background To evaluate the association between sports activities and allergic symptoms, especially rhinitis, among schoolchildren. Methods This longitudinal survey of schoolchildren collected data from questionnaires regarding allergic symptoms based on the International Study of Asthma and Allergies in Childhood (ISAAC) program and sports participation that were distributed to the parents of children at all 12 public primary schools in Ohmi-Hachiman City, Shiga Prefecture, Japan. Data were collected annually from 2011 until 2014, when the children reached 10 years of age. Blood samples were obtained in 2014, and the levels of immunoglobulin (Ig)E specific to four inhalant allergens were measured. Results Data from 558 children were analyzed. At 10 years of age, prevalence of asthma and eczema did not differ significantly, while rhinitis was significantly higher (p = 0.009) among children who participated in sports. Prevalence of rhinitis increased as the frequency or duration of sports participation increased (p < 0.01). The prevalence of new-onset rhinitis increased significantly among 10-year-olds with increasing duration of participation in sports (p = 0.03). Among those who participated in continuous sports activities, the prevalence of rhinitis was significantly higher with prolonged eczema (p = 0.006). Sports activities did not increase sensitization to inhalant allergens. Conclusion Sports activities enhance the prevalence of rhinitis in schoolchildren. Prolonged eczema, together with sports participation, further promotes the symptoms. The mechanisms of these novel findings warrant further investigation.
- Published
- 2015
33. Allergic status of schoolchildren with food allergy to eggs, milk or wheat in infancy
- Author
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Toshio Heike, Takeshi Morimoto, Tatsutoshi Nakahata, Tatsuya Fujii, Ryuta Nishikomori, and Takashi Kusunoki
- Subjects
Hypersensitivity, Immediate ,Male ,medicine.medical_specialty ,Allergy ,Adolescent ,Immunology ,Atopy ,Food allergy ,Surveys and Questionnaires ,Environmental health ,Epidemiology ,Prevalence ,medicine ,Animals ,Humans ,Immunology and Allergy ,Child ,Egg Hypersensitivity ,Triticum ,Asthma ,business.industry ,Atopic dermatitis ,Odds ratio ,Allergens ,medicine.disease ,Health Surveys ,Allergic conjunctivitis ,Milk ,Pediatrics, Perinatology and Child Health ,Female ,Milk Hypersensitivity ,business ,Food Hypersensitivity - Abstract
Although children allergic to eggs, milk or wheat in infancy tend to become tolerant by school age, the allergic status of these children at school age has not been well evaluated. To investigate the allergic status of schoolchildren who avoided eggs, milk or wheat because of an immediate-type allergic reaction at
- Published
- 2009
34. Tamoxifen inhibits tumor cell invasion and metastasis in mouse melanoma through suppression of PKC/MEK/ERK and PKC/PI3K/Akt pathways
- Author
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Takao Satou, Takashi Kusunoki, Shozo Nishida, Masanobu Tsubaki, Yuzuru Yamazoe, Tatsuki Itoh, Mitsuhiko Ogaki, and Hiroshi Matsuoka
- Subjects
MAPK/ERK pathway ,Lung Neoplasms ,Antineoplastic Agents, Hormonal ,Melanoma, Experimental ,Biology ,Mice ,Phosphatidylinositol 3-Kinases ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Neoplasm Invasiveness ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase A ,Protein kinase B ,Protein Kinase C ,PI3K/AKT/mTOR pathway ,Protein kinase C ,Kinase ,Cell Biology ,Mice, Inbred C57BL ,Tamoxifen ,Cancer research ,Mitogen-Activated Protein Kinases ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Neoplasm Transplantation ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction ,medicine.drug - Abstract
In melanoma, several signaling pathways are constitutively activated. Among these, the protein kinase C (PKC) signaling pathways are activated through multiple signal transduction molecules and appear to play major roles in melanoma progression. Recently, it has been reported that tamoxifen, an anti-estrogen reagent, inhibits PKC signaling in estrogen-negative and estrogen-independent cancer cell lines. Thus, we investigated whether tamoxifen inhibited tumor cell invasion and metastasis in mouse melanoma cell line B16BL6. Tamoxifen significantly inhibited lung metastasis, cell migration, and invasion at concentrations that did not show anti-proliferative effects on B16BL6 cells. Tamoxifen also inhibited the mRNA expressions and protein activities of matrix metalloproteinases (MMPs). Furthermore, tamoxifen suppressed phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt through the inhibition of PKCalpha and PKCdelta phosphorylation. However, other signal transduction factor, such as p38 mitogen-activated protein kinase (p38MAPK) was unaffected. The results indicate that tamoxifen suppresses the PKC/mitogen-activated protein kinase kinase (MEK)/ERK and PKC/phosphatidylinositol-3 kinase (PI3K)/Akt pathways, thereby inhibiting B16BL6 cell migration, invasion, and metastasis. Moreover, tamoxifen markedly inhibited not only developing but also clinically evident metastasis. These findings suggest that tamoxifen has potential clinical applications for the treatment of tumor cell metastasis.
- Published
- 2009
35. Macrophage inflammatory protein-1α (MIP-1α) enhances a receptor activator of nuclear factor κB ligand (RANKL) expression in mouse bone marrow stromal cells and osteoblasts through MAPK and PI3K/Akt pathways
- Author
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Shozo Nishida, Takao Satou, Takashi Kusunoki, Kimiko Fujiwara, Miyuki Manno, Masanobu Tsubaki, Hiroshi Matsuoka, Chisato Kato, Mitsuhiko Ogaki, Yoshihiro Tanimori, and Tatsuki Itoh
- Subjects
musculoskeletal diseases ,MAPK/ERK pathway ,medicine.medical_specialty ,Stromal cell ,Pyridines ,Morpholines ,Clinical Biochemistry ,Osteoclasts ,Bone Marrow Cells ,Mice ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,Internal medicine ,Nitriles ,Butadienes ,medicine ,Animals ,LY294002 ,Enzyme Inhibitors ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Molecular Biology ,Protein kinase B ,Cells, Cultured ,PI3K/AKT/mTOR pathway ,Chemokine CCL3 ,Osteoblasts ,biology ,RANK Ligand ,Imidazoles ,Cell Differentiation ,Osteoblast ,Cell Biology ,General Medicine ,Cell biology ,Oncogene Protein v-akt ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Chromones ,RANKL ,biology.protein ,Bone marrow ,Stromal Cells ,Signal Transduction - Abstract
Osteolytic lesions are rapidly progressive during the terminal stages of myeloma, and the bone pain or bone fracture that occurs at these lesions decreases the patients' quality of life to a notable degree. In relation to the etiology of this bone destruction, it has been reported recently that MIP-1alpha, produced in large amounts in myeloma patients, acts indirectly on osteoclastic precursor cells, and activates osteoclasts by way of bone-marrow stromal cells or osteoblasts, although the details of this process remain obscure. In the present study, our group investigated the mechanism by which RANKL expression is induced by MIP-1alpha and the effects of MIP-1alpha on the activation of osteoclasts. RANKL mRNA and RANKL protein expressions increased in both ST2 cells and MC3T3-E1 cells in a MIP-1alpha concentration-dependent manner. RANKL mRNA expression began to increase at 1 h after the addition of MIP-1alpha; the increase became remarkable at 2 h, and continuous expression was observed subsequently. Both ST2 and MC3T3-E1 cells showed similar levels of increased RANKL protein expression at 1, 2, and 3 days after the addition of MIP-1alpha. After the addition of MIP-1alpha, the amount of phosphorylated ERK1/2 and Akt protein expressions showed an increase, as compared to the corresponding amount in the control group. On the other hand, the amount of phosphorylated p38MAPK protein expression showed a decrease from the amount in the control group after the addition of MIP-1alpha. U0126 (a MEK1/2 inhibitor) or LY294002 (a PI3K inhibitor) was added to ST2 and MC3T3-E1 cells, and was found to inhibit RANKL mRNA and RANKL protein expression in these cells. When SB203580, a p38MAPK inhibitor, was added, RANKL mRNA and RANKL protein expression were increased in these cells. MIP-1alpha was found to promote osteoclastic differentiation of C7 cells, an osteoclastic precursor cell line, in a MIP-1alpha concentration-dependent manner. MIP-1alpha promoted differentiation into osteoclasts more extensively in C7 cells incubated together with ST2 and MC3T3-E1 cells than in C7 cells incubated alone. These results suggested that MIP-1alpha directly acts on the osteoclastic precursor cells and induces osteoclastic differentiation. This substance also indirectly induces osteoclastic differentiation through the promotion of RANKL expression in bone-marrow stromal cells and osteoblasts. The findings of this investigation suggested that activation of the MEK/ERK and the PI3K/Akt pathways and inhibition of p38MAPK pathway were involved in RANKL expression induced by MIP-1alpha in bone-marrow stromal cells and osteoblasts. This finding may be useful in the development of an osteoclastic inhibitor that targets intracellular signaling factors.
- Published
- 2007
36. Relationship between physique and food avoidance in infants: a study conducted in a community setting in Japan
- Author
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Takashi Kusunoki, Kiyoko Odani, Akane Higashi, Hiroaki Asano, Yukari Nishi, Yurika Tanaka, Kiyomi Harada, Sayuri Fukuda, and Ayako Saruwatari
- Subjects
Hypersensitivity, Immediate ,Male ,Pediatrics ,medicine.medical_specialty ,growth ,maternity passbook ,General Biochemistry, Genetics and Molecular Biology ,Percentile rank ,Child Development ,Japan ,Surveys and Questionnaires ,Weight growth ,Medicine ,Humans ,Longitudinal Studies ,infancy ,Retrospective Studies ,community health ,business.industry ,Body Weight ,Infant ,General Medicine ,Allergens ,Body Height ,Nutrition Assessment ,Child, Preschool ,Community health ,Community setting ,food avoidance ,Female ,business ,Body mass index ,Food Hypersensitivity ,Demography - Abstract
The relationship between food avoidance during infancy and the growth of Japanese infants in a community health setting has not been well evaluated. In order to assess the growth of infants who avoided either of the three major allergen foods in Japan, eggs, milk or wheat, we employed the results of 4 physical checkups recorded in maternity passbooks and administrated a questionnaire on allergic diseases, height and weight at birth to the guardians of 1,132 infants at the age of 3.5 years. Data was obtained from 890 subjects (78.6%) and 662 subjects (58.5%) who met the inclusion criteria were analyzed. The height, weight and body mass index percentile scores of each subject were calculated. Subjects who avoided either of the three foods at 3.5 years had lower weight percentile scores at 1.5 years, lower height and weight percentile scores at 3.5 years, and lower weight growth rates, compared with the subjects who did not avoid any of the three foods at 3.5 years (P=0.02, 0.03, 0.03, 0.01). The results suggested that there was a negative relationship between physique and food avoidance in infants, and that physical and nutritional assessments are important for food avoiders.
- Published
- 2015
37. [Untitled]
- Author
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Takashi Kusunoki, Tomoko Miyajima, Toshiyuki Kitoh, Tatsuya Fujii, and Masatoshi Ito
- Published
- 2006
38. CpG inhibits IgE class switch recombination through suppression of NFκB activity, but not through Id2 or Bcl6
- Author
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Akira Shimizu, Natsuki Nagata-Nakajima, Hiroyuki Gonda, Takashi Kusunoki, Yukiko Nambu, Mariko Kusunoki, Tomoya Katakai, Takeshi Tokuhisa, Manabu Sugai, Tatsutoshi Nakahata, Akemi Sakamoto, and Yoshifumi Yokota
- Subjects
Oligonucleotides ,Biophysics ,chemical and pharmacologic phenomena ,Biology ,Immunoglobulin E ,Biochemistry ,Mice ,chemistry.chemical_compound ,Transcription (biology) ,Proto-Oncogene Proteins ,Animals ,Molecular Biology ,Cells, Cultured ,Interleukin 4 ,Inhibitor of Differentiation Protein 2 ,B-Lymphocytes ,NF-kappa B ,Cell Biology ,BCL6 ,Molecular biology ,Immunoglobulin Switch Region ,DNA-Binding Proteins ,Repressor Proteins ,chemistry ,CpG site ,Immunoglobulin class switching ,Proto-Oncogene Proteins c-bcl-6 ,biology.protein ,CpG Islands ,Interleukin-4 ,DNA ,Signal Transduction ,Transcription Factors ,IRF4 - Abstract
The CpG motif in DNA plays a critical role in immunity via modulating the Th1/Th2 balance. In B cells, CpG-containing oligodeoxynucleotides (CpG ODNs) inhibit IL-4-mediated class switch recombination (CSR) to IgG1 and IgE through inhibition of the germline transcription (GLT) of these isotypes. However, the molecular mechanism of this inhibitory effect remains elusive. We showed here that Id2 and Bcl6, both of which inhibit IgE GLT and CSR, are not involved in this inhibitory pathway. We demonstrated that there is reduced activity of NF kappa B binding to the IgE promoter and a reduction of Irf4 protein in CpG ODN-treated B cells. These data indicate the critical role of NF kappa B and Irf4 in the regulation of IgE CSR through actions downstream of CpG signaling.
- Published
- 2005
39. Limited Ability of Antigen-Specific Th1 Responses to Inhibit Th2 Cell Development In Vivo
- Author
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Takahiro Yasumi, Ryuta Nishikomori, Takakazu Yoshioka, Ikuo Okafuji, Taka-aki Meguro, Toshio Heike, Takashi Kusunoki, Tatsutoshi Nakahata, and Kenji Katamura
- Subjects
Adoptive cell transfer ,CD8 Antigens ,Cellular differentiation ,Immunology ,Epitopes, T-Lymphocyte ,Mice, Transgenic ,chemical and pharmacologic phenomena ,Cell Communication ,Immunoglobulin E ,Resting Phase, Cell Cycle ,Interferon-gamma ,Mice ,Th2 Cells ,medicine ,Animals ,Immunology and Allergy ,Interferon gamma ,Sensitization ,Interleukin 4 ,Immunosuppression Therapy ,Mice, Inbred BALB C ,biology ,Cell growth ,Cell Differentiation ,hemic and immune systems ,Dendritic Cells ,T-Lymphocytes, Helper-Inducer ,Dendritic cell ,Th1 Cells ,Adoptive Transfer ,Growth Inhibitors ,medicine.anatomical_structure ,Immunoglobulin G ,biology.protein ,Interleukin-4 ,Spleen ,medicine.drug - Abstract
Th1 and Th2 cells mutually antagonize each other’s differentiation. Consequently, allergen-specific Th1 cells are believed to be able to suppress the development of Th2 cells and to prevent the development of atopic disorders. To determine whether a pre-existing Ag-specific Th1 response can affect the development of Th2 cells in vivo, we used an immunization model of Ag-pulsed murine dendritic cell (DC) transfer to induce distinct Th responses. When transferred into naive mice, Ag-pulsed CD8α+ DCs induced a Th1 response and the production of IgG2a, whereas CD8α− DCs primed a Th2 response and the production of IgE. In the presence of a pre-existing Ag-specific Th2 environment due to Ag-pulsed CD8α− DC transfer, CD8α+ DCs failed to prime Th1 cells. In contrast, CD8α− DCs could prime a Th2 response in the presence of a pre-existing Ag-specific Th1 environment. Moreover, exogenous IL-4 abolished the Th1-inducing potential of CD8α+ DCs in vitro, but the addition of IFN-γ did not effectively inhibit the potential of CD8α− DCs to prime IL-4-producing cells. Thus, Th1 and Th2 cells differ in their potential to inhibit the development of the other. This suggests that the early induction of allergen-specific Th1 cells before allergy sensitization will not prevent the development of atopic disorders.
- Published
- 2005
40. Somatic mosaicism ofCIAS1 in a patient with chronic infantile neurologic, cutaneous, articular syndrome
- Author
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Ryuta Nishikomori, Akihiro Fujisawa, Tatsutoshi Nakahata, Takakazu Yoshioka, Ikuo Okafuji, Naotomo Kambe, Momoko Yoshimoto, Takashi Kusunoki, Toshio Heike, Mami Nakata-Hizume, Katsuyuki Ohmori, Yoshiki Miyachi, and Megumu K. Saito
- Subjects
Mutation ,Pathology ,medicine.medical_specialty ,business.industry ,Point mutation ,Immunology ,Heterologous ,medicine.disease_cause ,medicine.disease ,Peripheral blood mononuclear cell ,Exon ,Rheumatology ,Familial Cold Autoinflammatory Syndrome ,Polymorphism (computer science) ,Immunology and Allergy ,Medicine ,Pharmacology (medical) ,business ,Meningitis - Abstract
Chronic infantile neurologic, cutaneous, articular syndrome (CINCA syndrome) is a severe inflammatory disease that was recently found to be associated with mutations in CIAS1. However, CIAS1 mutations have been detected in only half of CINCA syndrome patients, and it remains unclear which genes are responsible for the syndrome in the remaining patients. We describe here a patient with CINCA syndrome who exhibited CIAS1 somatic mosaicism. We genetically analyzed the CIAS1 gene in various blood cells and the buccal mucosa of the patient. The production of interleukin-1β (IL-1β) by peripheral blood mononuclear cells (PBMCs) was measured by enzyme-linked immunosorbent assay, and the ability of the mutant CIAS1 gene to enhance ASC-dependent NF-κB activation was assessed to confirm that the mutations of CIAS1 found were responsible for the patient's clinical manifestations of the CINCA syndrome. The patient had 1 heterologous single-nucleotide polymorphism, 587G>A (S196N), and 1 heterologous mutation, 1709A>G (Y570C), in exon 3 of CIAS1. The latter mutation was found to occur as somatic mosaicism. The patient's PBMCs produced a large amount of IL-1β in the absence of stimulation, unlike those from controls or from his mother, who also bore the S196N polymorphism. In addition, the Y570C mutation (with or without the S196N polymorphism) increased the ability of CIAS1 to induce ASC-dependent NF-κB activation, unlike the wild-type gene or the gene bearing the S196N polymorphism alone. The findings in this patient indicate that somatic mosaicism is one reason CIAS1 mutations have not been detected in some patients with CINCA syndrome.
- Published
- 2005
41. [Untitled]
- Author
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M Morita, Takashi Kusunoki, and Mitsuhiko Nambu
- Subjects
Inhalation ,business.industry ,On demand ,Disodium cromoglycate ,Medicine ,Pharmacology ,business - Published
- 2004
42. PROPOSAL OF NEW HIGH PERFORMANCE STEELS FOR BRIDGES (BHS500, BHS700)
- Author
-
Atsushi Ichikawa, Fumimaru Kawabata, Chitoshi Miki, and Takashi Kusunoki
- Subjects
Toughness ,Materials science ,Weldability ,Metallurgy ,engineering ,High strength steel ,Weathering steel ,engineering.material ,Bridge (interpersonal) - Abstract
最近, 様々な優れた特性を持つ鋼材が開発されている. しかし, それらの鋼材は, 必ずしも橋梁の性能と適合したものになっているわけではない. 橋梁の性能に適合した鋼材を選定することができれば, 橋梁の性能向上のほか, 橋梁の建設コストを縮減することも可能になる.本論文では, 日本における高強度鋼材の開発と使用の歴史をレヴューしたうえで, 橋梁用鋼材として求められる性能をまとめ, その性能を満足する鋼材を橋梁用高性能鋼材BHS500, BHS500WおよびBHS700として提案する. BHS500およびBHS500Wについては, 各種特性試験を行って要求性能を満足することを確認するとともに, これらの鋼材を用いるとどの程度のコスト縮減効果が得られるかを試設計により検討した.
- Published
- 2003
43. TH2 dominance and defective development of a CD8+ dendritic cell subset in Id2-deficient mice
- Author
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Akira Shimizu, Kayo Inaba, Tomonori Iyoda, Yoshiki Omatsu, Takashi Kusunoki, Yoshifumi Yokota, Tomoya Katakai, Manabu Sugai, and Tatsutoshi Nakahata
- Subjects
CD4-Positive T-Lymphocytes ,Cellular differentiation ,Immunology ,Gene Expression ,Spleen ,CD8-Positive T-Lymphocytes ,Immunoglobulin E ,Mice ,Th2 Cells ,T-Lymphocyte Subsets ,medicine ,Splenocyte ,Animals ,Immunology and Allergy ,Inhibitor of Differentiation Protein 2 ,Mice, Knockout ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Differentiation ,Dendritic Cells ,Dendritic cell ,T lymphocyte ,Mice, Mutant Strains ,DNA-Binding Proteins ,Repressor Proteins ,medicine.anatomical_structure ,biology.protein ,Antibody ,Cell Division ,CD8 ,Transcription Factors - Abstract
Background: Although the T H 1/T H 2 balance is important in many clinical situations, the regulatory mechanisms in vivo have not been well elucidated. Objective: We sought to characterize the immunologic status of mice lacking Id2, an inhibitor of basic helix-loop-helix transcription factors. Methods: We analyzed serum immunoglobulin levels, gene-expression profiles in the spleen, T H 1/T H 2 balance, and dendritic cell (DC) populations of Id2 –/– mice. Results: Serum levels of T H 2-mediated IgG1 and IgE were increased more than 10-fold in Id2 –/– mice without antigenic stimulation. Gene-expression analysis in Id2 –/– splenocytes revealed enhanced expression of T H 2-related genes, such as IL-4, and reduced expression of T H 1-related genes, including IFN-γ and IL-12. Intracellular cytokine staining also confirmed that Id2 –/– splenic CD4 + T cells are substantially skewed to T H 2 cells. However, Id2 –/– naive CD4 + T cells differentiated into T H 1 cells comparably with wild-type T cells under the appropriate culture conditions. Id2 –/– mice displayed a selective and remarkable reduction of the CD8α + DC subset, which is known to induce preferential T H 1 differentiation. Conclusion: Id2 is an indispensable regulator of the T H 1/T H 2 balance, possibly through the proper development of CD8α + DCs, and could be a novel target to treat allergic diseases. J Allergy Clin Immunol 2003;111:136-42.
- Published
- 2003
44. Essential role of Id2 in negative regulation of IgE class switching
- Author
-
Yoshifumi Yokota, Takashi Kusunoki, Tomoya Katakai, Manabu Sugai, Hiroyuki Gonda, and Akira Shimizu
- Subjects
Transcriptional Activation ,Immunology ,chemical and pharmacologic phenomena ,In Vitro Techniques ,Biology ,Immunoglobulin E ,Inhibitory postsynaptic potential ,Germline ,Transforming Growth Factor beta1 ,Mice ,Immune system ,Transforming Growth Factor beta ,Transcription (biology) ,Basic Helix-Loop-Helix Transcription Factors ,Hypersensitivity ,Animals ,Humans ,Immunology and Allergy ,Promoter Regions, Genetic ,Inhibitor of Differentiation Protein 2 ,Mice, Knockout ,B-Lymphocytes ,Base Sequence ,ALLERGIC/HYPERSENSITIVITY ,DNA ,Serum concentration ,Immunoglobulin Class Switching ,DNA-Binding Proteins ,Repressor Proteins ,Immunoglobulin class switching ,biology.protein ,Protein Binding ,Transcription Factors - Abstract
Serum concentrations of immunoglobulin E (IgE) in normal circumstances are kept much lower than those of other Ig isotypes to avoid allergic reactions. B cells lacking Id2 have increased E2A activity, which leads to specific enhancement of germline transcription of the immunoglobulin epsilon locus. As a consequence, Id2-deficient B cells undergo class switch recombination (CSR) to IgE at a much higher frequency than wild-type B cells. In contrast, Id2 is induced in wild-type B cells by transforming growth factor-beta1 (TGF-beta1) and suppresses IgE CSR. Our results provide evidence for the inhibitory and selective role of Id2 in IgE CSR in response to TGF-beta1. Id2 might act as molecular safeguard to suppress IgE CSR to prevent serious complications such as allergic hypersensitivity during the normal course of immune responses.
- Published
- 2002
45. Fruit Intake Significantly Reduces the Onset of Allergic Symptoms in Schoolchildren
- Author
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Takahiro Yasumi, Ryuta Nishikomori, Jiro Takeuchi, Takeshi Morimoto, Toshio Heike, Akane Higashi, Mio Sakuma, and Takashi Kusunoki
- Subjects
Allergic symptoms ,business.industry ,Immunology ,Immunology and Allergy ,Physiology ,Fruit intake ,Medicine ,business - Published
- 2017
46. Total and Low-Density Lipoprotein Cholesterol Levels are Associated with Atopy in Schoolchildren
- Author
-
Takahiro Yasumi, Ryuta Nishikomori, Takashi Kusunoki, Kumiko Mukaida, Mio Sakuma, Tatsuya Fujii, Takeshi Morimoto, and Toshio Heike
- Subjects
Male ,Physiology ,Low density lipoprotein cholesterol ,Hyperlipidemias ,Comorbidity ,Cohort Studies ,Atopy ,Allergic sensitization ,Age Distribution ,Japan ,Total cholesterol ,Hyperlipidemia ,Hypersensitivity ,Prevalence ,medicine ,Humans ,Mass Screening ,Sex Distribution ,Child ,School Health Services ,Lipoprotein cholesterol ,business.industry ,Cholesterol, HDL ,Cholesterol, LDL ,medicine.disease ,Obesity ,Cholesterol ,Logistic Models ,Multivariate Analysis ,Pediatrics, Perinatology and Child Health ,Population data ,Female ,business - Abstract
Analysis of general childhood population data showed that there was a significant positive association between total and low-density lipoprotein cholesterol levels and atopy, independent of obesity or sex, which suggest a relationship between hyperlipidemia and greater allergic sensitization among schoolchildren.
- Published
- 2011
47. Collision-free formation control of two-wheeled vehicles based on triangular LQ optimal regulator
- Author
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Yuh Yamashita, Daisuke Tsubakino, and Takashi Kusunoki
- Subjects
Control theory ,Computer science ,Collision free ,Regulator - Published
- 2014
48. Fever of unknown origin as the initial manifestation of valproate-induced Fanconi syndrome
- Author
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Kei Murayama, Akira Ohtake, Tomohiro Kumada, Fumihito Nozaki, Takashi Kusunoki, and Tatsuya Fujii
- Subjects
Male ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,Adolescent ,Urinary system ,Cytochrome-c Oxidase Deficiency ,Gastroenterology ,Tachypnea ,Asymptomatic ,Fever of Unknown Origin ,Developmental Neuroscience ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Fever of unknown origin ,Hypouricemia ,Enzyme Inhibitors ,business.industry ,Valproic Acid ,nutritional and metabolic diseases ,Fanconi syndrome ,medicine.disease ,Fanconi Syndrome ,Hypokalemia ,Endocrinology ,Neurology ,Pediatrics, Perinatology and Child Health ,Neurology (clinical) ,medicine.symptom ,business ,Hypophosphatemia - Abstract
Background Valproate-induced Fanconi syndrome is a rare adverse effect of valproate. Severely disabled patients who require tube feeding are reported to be susceptible to valproate-induced Fanconi syndrome. Although most patients with valproate-induced Fanconi syndrome are asymptomatic and detected incidentally with findings such as hypophosphatemia, hypouricemia, increased urinary β2-microglobulin, and generalized hyperaminoaciduria, clinical symptoms such as bone fracture, fever, tachypnea, and edema have been reported. Patient description This 15-year-old, severely disabled, tube-fed, male patient with cytochrome oxidase deficiency had taken valproate for 3 years when he developed fever for 3 weeks. Hypophosphatemia, hypouricemia, hypokalemia, increased urinary β2-microglobulin, and generalized hyperaminoaciduria, as well as hypocarnitinemia, were found, indicating that he had Fanconi syndrome. Valproate was the most likely cause of Fanconi syndrome in this patient. After discontinuation of valproate, the fever resolved immediately, and the laboratory findings normalized. Conclusion Valproate-induced Fanconi syndrome should be considered when individuals taking valproate develop fever of unknown origin.
- Published
- 2014
49. Distinct Aggregation of β- and γ-Chains of the High-affinity IgE Receptor on Cross-Linking
- Author
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Koichi Asai, Chizuka Ide, Takashi Kusunoki, Chisei Ra, Seigo Korematsu, Susumu Hosoi, Masashi Harazaki, and Kazushi Fujimoto
- Subjects
0301 basic medicine ,Histology ,media_common.quotation_subject ,Protein subunit ,Immunoglobulin E ,Cell membrane ,03 medical and health sciences ,Tumor Cells, Cultured ,medicine ,Animals ,Freeze Fracturing ,Microscopy, Immunoelectron ,Internalization ,Receptor ,media_common ,Membranes ,030102 biochemistry & molecular biology ,biology ,Receptors, IgE ,Chemistry ,Degranulation ,Immunogold labelling ,Molecular biology ,Rats ,Microscopy, Electron ,030104 developmental biology ,medicine.anatomical_structure ,Microscopy, Fluorescence ,biology.protein ,Biophysics ,Anatomy ,Cell activation - Abstract
The high-affinity IgE receptor (FcepsilonRI) on mast cells and basophils consists of a ligand-binding alpha-chain and two kinds of signaling chains, a beta-chain and disulfide-linked homodimeric gamma-chains. Crosslinking by multivalent antigen results in the aggregation of the bound IgE/alpha-chain complexes at the cell surface, triggering cell activation, and subsequent internalization through coated pits. However, the precise topographical alterations of the signaling beta- and gamma-chains during stimulation remain unclarified despite their importance in ligand binding/signaling coupling. Here we describe the dynamics of FcepsilonRI subunit distribution in rat basophilic leukemia cells during stimulation as revealed by immunofluorescence and immunogold electron microscopy. Immunolocalization of beta- and gamma-chains was homogeneously distributed on the cell surfaces before stimulation, while crosslinking with multivalent antigen, which elicited optimal degranulation, caused a distinct aggregation of these signaling chains on the cell membrane. Moreover, only gamma- but not beta-chains were aggregated during the stimulation that evoked suboptimal secretion. These findings suggest that high-affinity IgE receptor beta- and gamma-chains do not co-aggregate but for the most part form homogenous aggregates of beta-chains or gamma-chains after crosslinking.
- Published
- 2000
50. Learning Automaton Computing of Function Optimization Problems
- Author
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Fei Qian, Shigeya Ikebou, Takashi Kusunoki, Jijun Wu, and Yue Zhao
- Subjects
Theoretical computer science ,Computer science ,Materials Science (miscellaneous) ,Function optimization problems ,Automaton - Published
- 2000
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