143 results on '"Tak Wah Wong"'
Search Results
2. Cordycepin Inhibits Enterovirus A71 Replication and Protects Host Cell from Virus-Induced Cytotoxicity through Adenosine Action Pathway
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Yi-Ping Lee, Chun-Keung Yu, Tak-Wah Wong, Li-Ching Chen, and Bu-Miin Huang
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cordycepin ,enterovirus A71 ,antiviral activity ,adenosine pathway ,traditional herbal medicine ,infectious disease ,Microbiology ,QR1-502 - Abstract
Enterovirus A71 (EV-A71) infection typically causes mild illnesses, such as hand-foot-and-mouth disease (HFMD), but occasionally leads to severe or fatal neurological complications in infants and young children. Currently, there is no specific antiviral treatment available for EV-A71 infection. Thus, the development of an effective anti-EV-A71 drug is required urgently. Cordycepin, a major bioactive compound found in Cordyceps fungus, has been reported to possess antiviral activity. However, its specific activity against EV-A71 is unknown. In this study, the potency and role of cordycepin treatment on EV-A71 infection were investigated. Results demonstrated that cordycepin treatment significantly reduced the viral load and viral ribonucleic acid (RNA) level in EV-A71-infected Vero cells. In addition, EV-A71-mediated cytotoxicity was significantly inhibited in the presence of cordycepin in a dose-dependent manner. The protective effect can also be extended to Caco-2 intestinal cells, as evidenced by the higher median tissue culture infectious dose (TCID50) values in the cordycepin-treated groups. Furthermore, cordycepin inhibited EV-A71 replication by acting on the adenosine pathway at the post-infection stage. Taken together, our findings reveal that cordycepin could be a potential antiviral candidate for the treatment of EV-A71 infection.
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- 2024
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3. A clinicopathological study of perianal paget disease: A single center-based cohort study and literature review
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Ping-Chen Hou, Chaw-Ning Lee, Tak-Wah Wong, Tzu-Chien Hsu, Cheng-Lin Wu, and Julia Yu-Yun Lee
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extramammary paget disease ,immunohistochemistry ,occult anorectal malignancy ,perianal paget disease ,primary ,secondary ,Dermatology ,RL1-803 - Abstract
Background: Perianal Paget disease (PPD), an uncommon extramammary Paget disease, is characterized by intraepidermal pagetoid spread of atypical Paget cells in the perianal skin. PPDs can be primary or secondary. Secondary PPDs have poorer prognosis due to progression of the underlying anorectal carcinoma. Objectives: We analyzed the clinicopathological features of PPDs to determine the primary versus secondary PPD. Methods: We reviewed the clinicopathological features, including evidence of underlying anorectal carcinomas, tumor immunoprofiles, treatments, and outcomes of 8 cases of PPD diagnosed in our department during 1992–2019. Results: Colonoscopy was performed in 6 cases; rectal adenocarcinoma and anal canal adenocarcinoma were detected in 2 cases each. Three patients had local recurrence(s). Based on the detection of underlying anorectal cancers and immunoprofiles, 2 cases were classified as primary (one with perianal squamous cell carcinoma), 4 secondary, and 2 inconclusive for primary or secondary PPD. The immunoprofiles were CK7(+)/CK20(−)/GCDFP-15(−)/CDX2(−) in the primary PPDs; CK7(+/−)/CK20(+)/GCDFP-15(−)/CDX2(+) in the secondary and inconclusive PPDs. Eventually, all patients with secondary PPD died of the disease; one primary PPD and one inclusive PPD cases died of unrelated causes. Conclusion: We report the clinicopathological features of 8 cases of PPD in Taiwanese and first describe differential CK7 expression in the epidermal and dermal tumor cells in 2 cases of secondary PPDs, which may provide a clue to the diagnosis of secondary PPD. Since an underlying anorectal carcinoma in PPDs may be undetectable by colonoscopy, it is essential to consider anoscopy and/or anal canal mucosal biopsy to search for an occult anorectal carcinoma.
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- 2022
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4. An Update on Recent Advances of Photodynamic Therapy for Primary Cutaneous Lymphomas
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Wei-Ting Liu, Han-Tang Wang, Yi-Hsuan Yeh, and Tak-Wah Wong
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photodynamic therapy ,primary cutaneous lymphoma ,CTCL ,mycosis fungoides ,CBCL ,Pharmacy and materia medica ,RS1-441 - Abstract
Primary cutaneous lymphomas are rare non-Hodgkin lymphomas consisting of heterogeneous disease entities. Photodynamic therapy (PDT) utilizing photosensitizers irradiated with a specific wavelength of light in the presence of oxygen exerts promising anti-tumor effects on non-melanoma skin cancer, yet its application in primary cutaneous lymphomas remains less recognized. Despite many in vitro data showing PDT could effectively kill lymphoma cells, clinical evidence of PDT against primary cutaneous lymphomas is limited. Recently, a phase 3 “FLASH” randomized clinical trial demonstrated the efficacy of topical hypericin PDT for early-stage cutaneous T-cell lymphoma. An update on recent advances of photodynamic therapy in primary cutaneous lymphomas is provided.
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- 2023
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5. Extended-release tofacitinib for refractory Behçet disease
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Chrong-Reen Wang, MD, PhD, Tak-Wah Wong, MD, PhD, Sheng-Min Hsu, MD, PhD, and Maya Saranathan.
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Medicine - Abstract
Abstract. Rationale:. Although single-cytokine inhibitors can be considered in treating severe or refractory Behçet disease (BD), these biologic agents are associated with potential therapeutic failure due to the multi-cytokine pathogenesis involving Th1- and Th17-type cytokines with activated Janus kinase/signal transducer and activator of transcription signaling pathways. Notably, there is an increasing trend toward the use of small-molecule targeted drug tofacitinib (TOF), a pan-Janus kinase inhibitor, with immediate-release formulations for treating patients with severe or refractory systemic vasculitis involving different vessel sizes. Despite no reported efficacy of extended-release formulations in refractory BD yet, such a dosage form has pharmacokinetic parameters that are comparable to those of conventional immediate-release formulations. Patient concerns and diagnosis:. We report the case of a 27-year-old local woman with recurrent manifestations of arthritis, orogential ulcerations, papulopustular lesions, and anterior uveitis. She was diagnosed with BD for more than 3 years, and received long-term corticosteroids plus immunosuppressants therapy with the complication of opportunistic candidiasis infection. Interventions and outcomes:. Under extended-release TOF 11 mg once-daily therapy, the patient achieved disease remission while sparing the use of corticosteroids during follow-up. Lessons:. Our clinical observations implicate the oral convenience and therapeutic efficacy of extended-release TOF formulations in controlling the disease activity of BD.
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- 2022
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6. The Association of Patent Ductus Arteriosus with Inflammation: A Narrative Review of the Role of Inflammatory Biomarkers and Treatment Strategy in Premature Infants
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Yu-Jen Wei, Rosie Hsu, Yung-Chieh Lin, Tak-Wah Wong, Chung-Dann Kan, and Jieh-Neng Wang
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ductus arteriosus ,intrauterine inflammation ,preterm infants ,chorioamnionitis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Patent ductus arteriosus (PDA) is a common cardiovascular complication that complicates clinical care in the intensive care of premature infants. Prenatal and postnatal infections and the inflammation process can contribute to PDA, and intrauterine inflammation is a known risk factor of PDA. A variety of inflammatory biomarkers have been reported to be associated with PDA. Chorioamnionitis induces the fetal inflammatory process via several cytokines that have been reported to be associated with the presence of PDA and may have a role in the vascular remodeling process or vessel dilation of the ductus. On the other hand, anti-inflammatory agents, such as antenatal steroids, decrease PDA incidence and severity in patients born to those with chorioamnionitis. Proinflammatory cytokines, which are expressed more significantly in preterm neonates and chorioamnionitis, are associated with the presence of PDA. In this review, we focus on the pathogenesis of PDA in preterm infants and the role of biomarkers associated with the perinatal inflammatory process.
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- 2022
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7. Childhood and adolescent psoriasis in Taiwan: A retrospective analysis from a single medical center
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Hsi Yen, Hsing-Jou Su, Thi-Tuong Vi Tran, Pei-Lun Kuo, Julia Yu-Yun Lee, and Tak-Wah Wong
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Adolescent psoriasis ,Asian ,childhood psoriasis ,Dermatology ,RL1-803 - Abstract
There are limited studies regarding childhood and adolescent psoriasis in Taiwan. A total of 86 pathologically confirmed cases diagnosed from 1989 to 2017 were retrospectively reviewed. Mean disease onset age was 10.51 years, and plaque psoriasis was the most common type. Compared to studies on Caucasian and other Asian populations, we found a lower estimated prevalence, higher rate of psoriasis limited to the nail at presentation, and higher prevalence of psoriatic arthritis. The most common comorbidities were related to atopy and metabolic syndrome. Positive family history of psoriasis and psoriasis preceded by infection were significantly associated with moderate-to-severe disease.
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- 2019
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8. Health-related quality of life among patients with moderate-to-severe plaque psoriasis in Taiwan
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Tsen-Fang Tsai, Ji-Chen Ho, Yi-Ju Chen, Pa-Fan Hsiao, Woan-Ruoh Lee, Ching-Chi Chi, Cheng-Che Lan, Rosaline Chung-Yee Hui, Yang-Chih Lin, Kuo-Chia Yang, Tak-Wah Wong, Hamm-Ming Sheu, Hsiu-Cheng Hsu, Gong-Yau Chu, and Yu-Huei Huang
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Dermatology ,RL1-803 - Abstract
Background/Objectives: Plaque psoriasis is a debilitating condition that significantly affects patient well-being. Limited data are available regarding the effect of psoriasis and treatment on health-related quality of life (HRQoL) and work ability among Taiwanese patients.To document and compare HRQoL, treatment satisfaction, and work disability among Taiwanese patients with current and past moderate-to-severe plaque psoriasis. Methods: This was a multicenter, non-interventional, cross-sectional study of adult patients with moderate-to-severe plaque psoriasis. During a single clinic visit, each patient was assessed for body surface area (BSA) involvement, Psoriasis Area Severity Index (PASI), Dermatology Life Quality Index (DLQI), Euro Quality of Life-5 Dimensions (EQ-5D), 10-level satisfaction scale for psoriasis treatment, and Working Productivity and Activity Impairment (WPAI). Multivariate regression was used to identify factors associated with HRQoL and work disability. Results: A total of 305 patients were included within the analysis. The mean PASI score was 11.83, and the mean BSA involvement was 20.90%. The mean EQ-5D score was 65.68 and the mean DLQI score was 12.55. Fewer than half of patients (45.68%) indicated they were satisfied with the standard therapy they were currently receiving. Among employed patients, the mean reduction in on-the-job effectiveness was 32.09% and the mean reduction in overall productivity was 33.48%. The regression analysis indicated that patients with more severe psoriasis defined by PASI scores show a greater impact in quality of life and impairment in work disability; and that patients who were satisfied with current standard treatment had a better quality of life. Conclusion: The effect of psoriasis on HRQoL among patients with psoriasis in Taiwan is substantial, with fewer than half of patients reporting satisfaction with therapeutic options. Keywords: DLQI, EQ-5D, Health-related quality of life, Psoriasis, WPAI
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- 2018
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9. Real-world efficacy of biological agents in moderate-to-severe plaque psoriasis: An analysis of 75 patients in Taiwan.
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Yu-Chen Chen, Yi-Ting Huang, Chao-Chun Yang, Edward Chia-Cheng Lai, Cheng-Han Liu, Chao-Kai Hsu, Tak-Wah Wong, Sheau-Chiou Chao, Hamm-Ming Sheu, and Chaw-Ning Lee
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Medicine ,Science - Abstract
BackgroundReal-world clinical data on psoriasis patients receiving different biological agents is needed, especially in Asian populations.ObjectivesOur aim is to compare and analyze the efficacy and safety profile of four biological agents (etanercept, adalimumab, ustekinumab and secukinumab) in a real-world setting in Taiwan.MethodsWe retrospectively analyzed the clinical data of all patients with moderate-to-severe plaque psoriasis (Psoriasis Area and Severity Index (PASI) ≥ 10) who received etanercept, adalimumab, ustekinumab or secukinumab between January 2011 and December 2018 in a tertiary hospital in Taiwan.ResultsA total of 119 treatment episodes in 75 patients were included in this study. Ustekinumab was used in 49 treatment episodes, followed by secukinumab in 46 treatment episodes, adalimumab in 14 treatment episodes and etanercept in 10 treatment episodes. The proportion of the biologic-naïve was highest in etanercept (100%) and lowest in secukinumab (23.9%). The PASI-75, -90 and -100 were the highest in secukinumab (91.3%, 82.6%, 41.3%, respectively), followed by ustekinumab (79.6%, 44.9%, 16.3%), adalimumab (64.3%, 28.6%, 7.1%) and etanercept (50.0%, 30.0%, 0%). The rate of adverse events that required treatment was highest for secukinumab (15.2%), followed by adalimumab (14.3%), ustekinumab (8.2%), and etanercept (0%), including 4 cases of infections, 2 cases of cardiovascular diseases and 4 cases of cancers.ConclusionsThis real world data showed differential efficacy and safety of the four biological agents.
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- 2020
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10. Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by indocyanine green and near infrared light
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Tak-Wah Wong, En-Chi Wu, Wen-Chien Ko, Ching-Chi Lee, Lien-I Hor, and I-Hsiu Huang
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Antibiotic-resistant ,Indocyanine green ,Methicillin-resistant Staphylococcus aureus ,Near infrared ,Photodynamic therapy ,Dermatology ,RL1-803 - Abstract
Background: Antimicrobial photodynamic therapy (APDT) has become a potential regimen to treat multidrug-resistant bacterial infections. Limited data showed indocyanine green (ICG), a safe and inexpensive contrast medium for eye angiography and hepatic function examination, is an effective photosensitizer in APDT to kill methicillin-resistant Staphylococcus aureus (MRSA) after excitation with laser. Objective: We investigated the potentials of ICG-APDT with an inexpensive, non-coherent commercial near infrared (NIR) lamp against MRSA. Methods: The inhibition of MRSA was studied after exposing bacteria to NIR with different light doses and concentrations of ICG. The selectivity on MRSA was examined on human fibroblasts. Bacterial virulence including the activities of coagulase and enterotoxin was investigated. The effects of singlet oxygen scavengers (tryptophan and ascorbic acid) and H2O2 on cell survival were evaluated. The morphology of bacteria after PDT was observed by transmission electron microscopy. Results: ICG-PDT inhibited the growth of bacteria by 5 log (99.999% inhibition) with 200 J/cm2 at 65.5 mW/cm2 in the presence of 100 μg/mL ICG. Adding 0.1% H2O2 at a lower PDT dose (25 μg/mL ICG and 100 J/cm2) increased its efficacy by 5 log. This PDT dose was not toxic to human fibroblasts. PDT significantly reduced the level of bacterial virulence factors. The inhibition effects were decreased by tryptophan and ascorbic acid suggested singlet oxygen involved in the process. TEM showed severe non-selective cell destruction immediately after irradiation. Conclusion: The study reveals ICG-PDT has the potential to treat MRSA by using a clinical accessible NIR lamp and photosensitizer.
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- 2018
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11. Recent Advances in Photodynamic Therapy against Fungal Keratitis
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Jia-Horung Hung, Chaw-Ning Lee, Huai-Wen Hsu, I-Son Ng, Chi-Jung Wu, Chun-Keung Yu, Nan-Yao Lee, Yun Chang, and Tak-Wah Wong
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candida ,collagen cross-linking ,drug delivery ,fungal infection ,flavin mononucleotide ,keratitis ,Pharmacy and materia medica ,RS1-441 - Abstract
Fungal keratitis is a serious clinical infection on the cornea caused by fungi and is one of the leading causes of blindness in Asian countries. The treatment options are currently limited to a few antifungal agents. With the increasing incidence of drug-resistant infections, many patients fail to respond to antibiotics. Riboflavin-mediated corneal crosslinking (similar to photodynamic therapy (PDT)) for corneal ectasia was approved in the US in the early 2000s. Current evidence suggests that PDT could have the potential to inhibit fungal biofilm formation and overcome drug resistance by using riboflavin and rose bengal as photosensitizers. However, only a few clinical trials have been initiated in anti-fungal keratitis PDT treatment. Moreover, the removal of the corneal epithelium and repeated application of riboflavin and rose bengal are required to improve drug penetration before and during PDT. Thus, an improvement in trans-corneal drug delivery is mandatory for a successful and efficient treatment. In this article, we review the studies published to date using PDT against fungal keratitis and aim to enhance the understanding and awareness of this research area. The potential of modifying photosensitizers using nanotechnology to improve the efficacy of PDT on fungal keratitis is also briefly reviewed.
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- 2021
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12. Microplasma Treatment versus Negative Pressure Therapy for Promoting Wound Healing in Diabetic Mice
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Pei-Lin Shao, Jiunn-Der Liao, Shun-Cheng Wu, Yu-Hsing Chen, and Tak-Wah Wong
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diabetic wound ,nonthermal microplasma treatment ,negative pressure wound therapy ,re-epithelialization ,transforming growth factor β signaling ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The delayed healing response of diabetic wounds is a major challenge for treatment. Negative pressure wound therapy (NPWT) has been widely used to treat chronic wounds. However, it usually requires a long treatment time and results in directional growth of wound healing skin tissue. We investigated whether nonthermal microplasma (MP) treatment can promote the healing of skin wounds in diabetic mice. Splint excision wounds were created on diabetic mice, and various wound healing parameters were compared among MP treatment, NPWT, and control groups. Quantitative analysis of the re-epithelialization percentage by detecting Ki67 and DSG1 expression in the extending epidermal tongue (EET) of the wound area and the epidermal proliferation index (EPI) was subsequently performed. Both treatments promoted wound healing by enhancing wound closure kinetics and wound bed blood flow; this was confirmed through histological analysis and optical coherence tomography. Both treatments also increased Ki67 and DSG1 expression in the EET of the wound area and the EPI to enhance re-epithelialization. Increased Smad2/3/4 mRNA expression was observed in the epidermis layer of wounds, particularly after MP treatment. The results suggest that the Smad-dependent transforming growth factor β signaling contributes to the enhancement of re-epithelialization after MP treatment with an appropriate exposure time. Overall, a short-term MP treatment (applied for 30 s twice a day) demonstrated comparable or better efficacy to conventional NPWT (applied for 4 h once a day) in promoting wound healing in diabetic mice. Thus, MP treatment exhibits promise for treating diabetic wounds clinically.
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- 2021
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13. Successful treatment of disseminated cutaneous lesions of classic type Kaposi sarcoma with low-dose peginterferon alfa-2a: A case report
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Wei-Ting Liu, Chaw-Ning Lee, Yin-Yu Ho, Hsiang-Ying Lu, Chia-Jui Yen, and Tak-Wah Wong
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Dermatology ,RL1-803 - Published
- 2021
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14. Taiwanese Dermatological Association consensus statement on management of psoriasis
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Tsen-Fang Tsai, Chih-Hung Lee, Yu-Huei Huang, Ching-Chi Chi, Yun-Ting Chang, Tak-Wah Wong, Chih-Hsun Yang, and Li-Fang Wang
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consensus development ,medication therapy management ,psoriasis ,Taiwanese Dermatological Association ,Dermatology ,RL1-803 - Abstract
Background: Effective management of psoriasis is a cause for much concern. In Taiwan, there is a lack of consensus on management strategies for psoriasis, especially the principles of drug prescribing in psoriatic patients. Objectives: The Taiwanese Dermatological Association convened Expert Panel meetings three times between 2012 and 2015 to discuss the management strategies for treatment of psoriasis in order to fill the knowledge gap and provide a reference tool for Taiwanese dermatologists. Results: This paper reports the final output from the three meetings, with the aim of aiding clinical decision making in terms of principles of prescribing, including dosing strategies, efficacy profiles, and safety concerns in connection with eight categories of antipsoriatic treatment: topical agents, phototherapy, nonbiologic conventional systemic agents, licensed biologic systemic agents, newly emerging therapies, combination therapy, transitional therapy, and traditional Chinese medicine. Conclusion: The Expert Panel, comprising distinguished Taiwanese dermatologists, succeeded in developing a consensus about the management of psoriasis in Taiwanese patients. Unavailability of data in certain areas may suggest a possibility of new directions in research.
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- 2017
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15. Recent Advances in Photodynamic Therapy for Deep-Seated Tumors with the Aid of Nanomedicine
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Wei-Peng Li, Chia-Jui Yen, Bo-Sheng Wu, and Tak-Wah Wong
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photodynamic therapy (PDT) ,photosensitizer ,hypoxia ,metal–organic framework (MOF) ,pancreatic cancer ,Biology (General) ,QH301-705.5 - Abstract
Photodynamic therapy (PDT) works through photoactivation of a specific photosensitizer (PS) in a tumor in the presence of oxygen. PDT is widely applied in oncology to treat various cancers as it has a minimally invasive procedure and high selectivity, does not interfere with other treatments, and can be repeated as needed. A large amount of reactive oxygen species (ROS) and singlet oxygen is generated in a cancer cell during PDT, which destroys the tumor effectively. However, the efficacy of PDT in treating a deep-seated tumor is limited due to three main reasons: Limited light penetration depth, low oxygen concentration in the hypoxic core, and poor PS accumulation inside a tumor. Thus, PDT treatments are only approved for superficial and thin tumors. With the advancement of nanotechnology, PDT to treat deep-seated or thick tumors is becoming a reachable goal. In this review, we provide an update on the strategies for improving PDT with nanomedicine using different sophisticated-design nanoparticles, including two-photon excitation, X-ray activation, targeting tumor cells with surface modification, alteration of tumor cell metabolism pathways, release of therapeutic gases, improvement of tumor hypoxia, and stimulation of host immunity. We focus on the difficult-to-treat pancreatic cancer as a model to demonstrate the influence of advanced nanomedicine in PDT. A bright future of PDT application in the treatment of deep-seated tumors is expected.
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- 2021
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16. Daylight Photodynamic Therapy: An Update
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Chaw-Ning Lee, Rosie Hsu, Hsuan Chen, and Tak-Wah Wong
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actinic keratosis ,aminolevulinic acid ,daylight ,photodynamic therapy ,history ,nonmelanoma skin cancer ,Organic chemistry ,QD241-441 - Abstract
Daylight photodynamic therapy (dPDT) uses sunlight as a light source to treat superficial skin cancer. Using sunlight as a therapeutic device has been present for centuries, forming the basis of photodynamic therapy in the 20th century. Compared to conventional PDT, dPDT can be a less painful, more convenient and an effective alternative. The first clinical uses of dPDT on skin cancers began in Copenhagen in 2008. Currently, aminolevulinic acid-mediated dPDT has been approved to treat actinic keratosis patients in Europe. In this review article, we introduce the history and mechanism of dPDT and focus on the pros and cons of dPDT in treating superficial skin cancers. The future applications of dPDT on other skin diseases are expected to expand as conventional PDT evolves.
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- 2020
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17. Personalized Immuno-Oncology with Immunodeficiency Mouse Models
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Jui-Ling Wang, Wen-Hui Ma, Tak-Wah Wong, and Chun-Keung Yu
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- 2023
18. Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts
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Chung Dann Kan, Stephanie Tsao, Tak Wah Wong, Shao-Hsien Lin, Ko-Chi Chang, and Jieh Neng Wang
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Embryology ,Pathology ,medicine.medical_specialty ,Swine ,Biomedical Engineering ,Biology ,Umbilical vein ,Adventitia ,medicine.artery ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,CD90 ,Progenitor cell ,Transplantation ,Decellularization ,Tissue Engineering ,Tissue Scaffolds ,Stem Cells ,Arteries ,Endoglin ,Blood Vessel Prosthesis ,medicine.anatomical_structure ,Pulmonary artery ,Research Paper ,Developmental Biology ,Artery - Abstract
To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.
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- 2021
19. Portable ultraviolet light A1 light source to treat hypertrophic scar
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Yen-Chen Huang, Chin-Te Huang, Chieh Hu, and Tak-Wah Wong
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fibrosis ,light-emitting diode ,phototherapy ,scar ,UV-A1 ,Dermatology ,RL1-803 - Abstract
Background/Objectives: Recently, fibrotic diseases such as hypertrophic scar, keloid, and scleroderma have been treated with UV-A1 radiation with encouraging results. However, conventional UV light sources are bulky and expensive. In this study, we aimed to verify the effectiveness of a portable UV-A1 radiation device in treating hypertrophic scars. Materials and methods: A light-emitting diode array that emitted 365 ± 5 nm (UV-A1) was used to irradiate fibroblasts and hypertrophic scar in a rabbit model. Results: In cell cultures, UV-A1 light exposure inhibited post-wound cell migration and reduced the total amount of soluble collagen production in fibroblasts. Type I collagenase production and its activity increased after treatment. On the rabbit ear, UV-A1 light irradiation reduced the thickness of hypertrophic scars, confirming the antifibrotic effect in vivo. Conclusion: These results support the potential of a portable UV-A1 light device in treating hypertrophic scar.
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- 2015
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20. Transplantation of autologous single hair units heals chronic wounds in autosomal recessive dystrophic epidermolysis bullosa: A proof-of-concept study
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Tak Wah Wong, Cheng Han Liu, Julia Yu-Yun Lee, Chao Kai Hsu, and Chao Chun Yang
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medicine.medical_specialty ,Mucocutaneous zone ,Dermatology ,Transplantation, Autologous ,Pathology and Forensic Medicine ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Follicular phase ,medicine ,Humans ,Autologous transplantation ,Ulcer ,Wound Healing ,integumentary system ,030504 nursing ,business.industry ,Middle Aged ,medicine.disease ,Hair follicle ,Epidermolysis Bullosa Dystrophica ,Transplantation ,medicine.anatomical_structure ,Scalp ,Female ,Epidermolysis bullosa ,0305 other medical science ,business ,Wound healing ,Hair Follicle - Abstract
Autosomal recessive dystrophic epidermolysis bullosa (RDEB) is characterized by recurrent mucocutaneous blistering with non-healing ulcers which are often complicated by squamous cell carcinoma (SCC). Despite having as high as 80% death rate from SCC, RDEB still does not have an effective treatment. We report on the efficacy of single follicular unit extract (FUE) grafting to heal chronic ulcers of intermediate RDEB in a 54-year-old woman with extensive chronic wounds covering around 30% of the body surface area. On Day 17 post first graft session, the area of treated ulcers on her right upper back was reduced by 80%. Immunofluorescence study revealed positive type VII collagen expression along the epidermal and follicular basement membrane zone in the donor and recipient sites. A few grafted follicles continued to grow hair on the recipient sites. A total of 360 FUEs were grafted in nine sessions over five years, resulting in healing of most treated ulcers and reduced significantly her time for daily wound dressing. Importantly, FUE grafting using patient's own scalp follicles does not require any laboratory manipulation. It is safe and easy to perform. Autologous follicular grafting appears efficacious for healing of recalcitrant wounds and provides an innovative solution for RDEB patients with such wounds.
- Published
- 2021
21. Effects of Solanum undatum extract (SR‐T100) on photocarcinogenesis and photoaging of actinic keratosis
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Tak Wah Wong, Juemin Zhao, Ying Jan Wang, Hamm Ming Sheu, Chao Hsing Kao, Cheng Che Eric Lan, Tzu Kai Lin, Kai Che Wei, Lei-Hong Xiang, Chao Chun Yang, Chin Han Wu, Chrang Shi Lin, and Feng Jie Lai
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Photoaging ,Human skin ,Dermatology ,Solanum ,Mice ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Solanum incanum ,Solamargine ,biology ,Plant Extracts ,Actinic keratosis ,Therapeutic effect ,General Medicine ,medicine.disease ,biology.organism_classification ,Molecular biology ,Skin Aging ,Keratosis, Actinic ,chemistry ,030220 oncology & carcinogenesis ,Immunohistochemistry ,Papilloma - Abstract
SR-T100 gel, containing solamargine extracted from Solanum undatum (synonym: Solanum incanum), had good therapeutic effects on actinic keratosis (AK) in human and ultraviolet B-induced papilloma in mice. This study aimed to investigate the immunohistochemical changes in the human skin after SR-T100 treatment. An immunohistochemical study was performed and the changes in photocarcinogenesis and photoaging markers after 16-week SR-T100 gel treatment were documented. SR-T100 gel treatment for 16 weeks resulted in complete remission in nine AK lesions and partial remission in four AK lesions. SR-T100 gel abolished the expression of mutant p53 and SOX2 and restored the expression of NOTCH1. Additionally, SR-T100 gel improved wrinkling in human skin, while restoring the expression of lamin B1 and increasing synthesis of new elastic fibers. SR-T100 gel had therapeutic effects on photocarcinogenesis and photoaging of photodamaged skin with AK.
- Published
- 2021
22. Imiquimod-induced ROS production causes lysosomal membrane permeabilization and activates caspase-8-mediated apoptosis in skin cancer cells
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Shu-Hao Chang, Pei-Ying Lin, Tsai-Kun Wu, Chien-Sheng Hsu, Shi-Wei Huang, Zheng-Yi Li, Kuang-Ting Liu, Jun-Kai Kao, Yi-Ju Chen, Tak-Wah Wong, Chun-Ying Wu, and Jeng-Jer Shieh
- Subjects
Caspase 8 ,Imiquimod ,Skin Neoplasms ,Apoptosis ,Dermatology ,DNA ,Ligands ,Biochemistry ,Antiviral Agents ,Cathepsins ,Sincalide ,Mice ,Toll-Like Receptor 7 ,Animals ,Humans ,Lysosomes ,Reactive Oxygen Species ,Molecular Biology ,Signal Transduction - Abstract
Lysosomal cell death is induced by lysosomal membrane permeabilization (LMP) and the subsequent release of lysosomal proteolytic enzymes, including cathepsins (CTSs), which results in mitochondrial dysfunction and apoptosis. Imiquimod (IMQ), a synthetic TLR7 ligand, has both antiviral and antitumor activity against various skin malignancies in clinical treatment. Previously, we demonstrated IMQ not only caused lysosomal dysfunction but also triggered lysosome biogenesis to achieve lysosomal adaptation in cancer cells.To determine whether lysosomes are involved in IMQ-induced apoptosis.The human skin cancer cell lines BCC, A375 and mouse melanoma cell line B16F10 were used in all experiments. Cell death was determined by the Cell Counting Kit-8 (CCK-8) assay and DNA content assay. Protein expression was determined by immunoblotting. Caspase-8 activity was assessed using a fluorescence caspase-8 kit and determined by flow cytometry and confocal microscopy.IMQ not only induced lysosome damage but also abrogated lysosome function in skin cancer cells. IMQ-induced caspase-8 activation contributed to the processes of lysosomal cell death. Moreover, the use of ROS scavengers significantly abolished caspase-8 activation and inhibited IMQ-induced LMP. Additionally, pharmacological inhibition of CTSD not only abrogated caspase-8 activation but also rescued IMQ-induced cell death. Finally, lysosome-alkalizing agents enhanced the cytotoxicity of IMQ in vitro and in vivo.IMQ-induced ROS accumulation promotes LMP, releases CTSs into the cytosol, stimulates caspase-8 activation and finally causes lysosomal cell death. Lysosomal cell death and the CTSD/caspase-8 axis may play a crucial role in IMQ-induced cell death.
- Published
- 2022
23. Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans
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Jia-Horung, Hung, Zhao-Xiang, Wang, Yuan-Hsin, Lo, Chaw-Ning, Lee, Yun, Chang, Rita Yuchiao, Chang, Chien-Chun, Huang, and Tak-Wah, Wong
- Subjects
Rose Bengal ,Antifungal Agents ,Photosensitizing Agents ,Anti-Infective Agents ,Photochemotherapy ,Biofilms ,Candida albicans ,Candidiasis ,Humans ,Female ,Anti-Bacterial Agents - Abstract
Invasive Candida albicans infection is a significant opportunistic fungal infection in humans because it is one of the most common colonizers of the gut, mouth, vagina, and skin. Despite the availability of antifungal medication, the mortality rate of invasive candidiasis remains ~50%. Unfortunately, the incidence of drug-resistant C. albicans is increasing globally. Antimicrobial photodynamic therapy (aPDT) may offer an alternative or adjuvant treatment to inhibit C. albicans biofilm formation and overcome drug resistance. Rose bengal (RB)-mediated aPDT has shown effective cell killing of bacteria and C. albicans. In this study, the efficacy of RB-aPDT on multidrug-resistant C. albicans is described. A homemade green light-emitting diode (LED) light source is designed to align with the center of a well of a 96-well plate. The yeasts were incubated in the wells with different concentrations of RB and illuminated with varying fluences of green light. The killing effects were analyzed by the plate dilution method. With an optimal combination of light and RB, 3-log growth inhibition was achieved. It was concluded that RB-aPDT might potentially inhibit drug-resistant C. albicans.
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- 2022
24. Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans
- Author
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Tak-Wah Wong, Chien-Chun Huang, Rita Yuchiao Chang, Yun Chang, Chaw-Ning Lee, Yuan-Hsin Lo, Zhao-Xiang Wang, and Jia-Horung Hung
- Subjects
General Immunology and Microbiology ,General Chemical Engineering ,General Neuroscience ,General Biochemistry, Genetics and Molecular Biology - Published
- 2022
25. The Immunogenetic Aspects of Photodynamic Therapy
- Author
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Chaw-Ning, Lee and Tak-Wah, Wong
- Subjects
Keratosis, Actinic ,Photosensitizing Agents ,Skin Neoplasms ,Photochemotherapy ,Immunogenetics ,Humans - Abstract
Photodynamic therapy (PDT) has become the first-line treatment of actinic keratosis, superficial basal cell carcinoma, and squamous cell carcinoma in situ (Bowen's disease) in dermatology. The off-label use of PDT has also escalated in recent years owing to its applications in the treatment of various non-neoplastic skin diseases such as acne vulgaris, vascular lesions, rejuvenation, and chronic wounds. Daylight PDT that uses natural sunlight to activate a photosensitizer with advantages such as low cost and reduced pain is widely used in Europe. This chapter reviews the applications and immunogenetic aspects of PDT. However, the studies of immunity and genetic changes in human tissue after PDT are limited.
- Published
- 2022
26. The Immunogenetics of Photodermatoses
- Author
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Chaw-Ning, Lee, Tzu-Ying, Chen, and Tak-Wah, Wong
- Subjects
Male ,Adolescent ,Ultraviolet Rays ,Immunogenetics ,Humans ,Photosensitivity Disorders - Abstract
Photodermatosis is an abnormal skin inflammatory reaction to light. The major classifications of photodermatoses are idiopathic photodermatoses, photodermatoses due to exogenous or endogenous agents, photo-exacerbated dermatoses, and photosensitive genodermatoses. In this chapter, we focus on idiopathic photodermatoses and drug-related photodermatoses and emphasize on the epidemiology and immunogenetic backgrounds. Idiopathic photodermatoses, a spectrum of diseases with abnormal responses to ultraviolet radiation (UVR), include polymorphous light eruption, actinic prurigo, hydroa vacciniforme, chronic actinic dermatitis, and solar urticaria. Young people are more susceptible to most idiopathic photodermatoses except for chronic actinic dermatitis. Interestingly, idiopathic photodermatoses exhibit different characteristics between Caucasians and Asians. For example, the average age of Asian actinic prurigo patients is older than that of Caucasians in which genetic backgrounds or Fitzpatrick skin type might play a role. Drug-induced photodermatoses can be classified into phototoxic and photoallergic drug reactions. Certain drug-induced photodermatoses may mimic other dermatoses. For instance, drug-induced lupus erythematosus (LE) should be considered if an old man is diagnosed with LE but had a poor response to standard treatments.
- Published
- 2022
27. Secondary intention healing with satisfactory outcome after nodular basal cell carcinoma excision on the face
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Jia-Ming Yeh, Chun-Yen Ou, Julia Yu-Yun Lee, and Tak-Wah Wong
- Subjects
cosmetic outcome ,nonmelanoma skin cancer ,secondary intention ,wound healing ,Dermatology ,RL1-803 - Abstract
Secondary intention healing on concave areas of the face may provide acceptable cosmetic outcome after tumor excision but is underused. We evaluated cosmetic outcome and tumor recurrence of this technique in 10 patients with nodular basal cell carcinoma and one patient with basosquamous carcinoma on the face. The average size of these tumors was 1 cm. Subjective evaluations included patients' satisfaction on the degree of wound pain, ease of wound care, and satisfaction with cosmetic outcome. Objective evaluations included physician's scoring on the time to complete wound healing, wound infection, cosmetic outcome, and tumor recurrence after operation. The operations were completed in 30 minutes on average. All wounds healed well without infection within 4 weeks. Postoperation wound pain was absent to mild. Wound care was neither difficult nor troublesome. All patients were satisfied with the cosmetic outcome. Physicians scored good or excellent cosmetic outcome in 91% of patients. No tumor recurred during 3–60 months (median, 13 months) of follow-up. Secondary intention healing appears to be a good option after excision of nodular basal cell carcinomas located on concave areas of the face. Good to excellent cosmetic results can be expected after wound healing.
- Published
- 2013
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28. Developing creativity in computer lessons.
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Yiu-chi Lai and Tak-wah Wong
- Published
- 2009
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29. Enhancement of Wound Healing by Non-Thermal N2/Ar Micro-Plasma Exposure in Mice with Fractional-CO2-Laser-Induced Wounds.
- Author
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Pei-Lin Shao, Jiunn-Der Liao, Tak-Wah Wong, Yi-Cheng Wang, Steve Leu, and Hon-Kan Yip
- Subjects
Medicine ,Science - Abstract
Micro-plasma is a possible alternative treatment for wound management. The effect of micro-plasma on wound healing depends on its composition and temperature. The authors previously developed a capillary-tube-based micro-plasma system that can generate micro-plasma with a high nitric oxide-containing species composition and mild working temperature. Here, the efficacy of micro-plasma treatment on wound healing in a laser-induced skin wound mouse model was investigated. A partial thickness wound was created in the back skin of each mouse and then treated with micro-plasma. Non-invasive methods, namely wound closure kinetics, optical coherence tomography (OCT), and laser Doppler scanning, were used to measure the healing efficiency in the wound area. Neo-tissue growth and the expressions of matrix metallopeptidase-3 (MMP-3) and laminin in the wound area were assessed using histological and immunohistochemistry (IHC) analysis. The results show that micro-plasma treatment promoted wound healing. Micro-plasma treatment significantly reduced the wound bed region. The OCT images and histological analysis indicates more pronounced tissue regrowth in the wound bed region after micro-plasma treatment. The laser Doppler images shows that micro-plasma treatment promoted blood flow in the wound bed region. The IHC results show that the level of laminin increased in the wound bed region after micro-plasma treatment, whereas the level of MMP-3 decreased. Based on these results, micro-plasma has potential to be used to promote the healing of skin wounds clinically.
- Published
- 2016
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30. The Immunogenetic Aspects of Photodynamic Therapy
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Chaw-Ning Lee and Tak-Wah Wong
- Published
- 2022
31. The Immunogenetics of Photodermatoses
- Author
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Chaw-Ning Lee, Tzu-Ying Chen, and Tak-Wah Wong
- Published
- 2022
32. Circumferential scouting punch biopsies to delineate surgical margin for dermatofibrosarcoma protuberans
- Author
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Tsung-Mao Huang, Shyh-Jou Shieh, J. Yu-Yun Lee, and Tak-Wah Wong
- Subjects
dermatofibrosarcoma protuberans ,excision margins ,scouting biopsy ,Dermatology ,RL1-803 - Abstract
Dermatofibrosarcoma protuberans (DFSP) is an uncommon soft-tissue tumor involving the dermis and subcutaneous tissue with a high local recurrence rate after standard excision. Mohs micrographic surgery offers a lower recurrence rate. However, the procedure requires multiple stages of excision with intraoperative histopathological mapping, which is time consuming and expensive. We report our experience of using circumferential scouting punch biopsy technique in five patients to determine in advance the resection margins for DFSP prior to wide excision. Multiple 4 mm punches, usually eight in number, were performed 1–2.5 cm around the palpable borders of DFSP to delineate the resection margins in five consecutive patients. Tumors were excised at a later date along the margin defined by these biopsies and the wounds were repaired with skin graft. The operation was completed in 2 hours in all cases excluding one that required frozen sections for deep margin. No recurrence was noted 2–10 years after the operations. The results suggest that circumferential scouting punch biopsies before wide excision may be an alternative method to define the resection margins for DFSP when Mohs surgery is not available.
- Published
- 2012
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33. Repair of a Large Scrotal Skin Defect
- Author
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Tak Wah Wong, Fu-Nien Hsieh, and Chaw Ning Lee
- Subjects
Male ,medicine.medical_specialty ,business.industry ,medicine ,Scrotal skin ,Scrotum ,Humans ,Surgery ,Dermatology ,General Medicine ,business ,Surgical Flaps - Published
- 2021
34. Recent Advances in Photodynamic Therapy against Fungal Keratitis
- Author
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Chun Keung Yu, Chaw Ning Lee, Chi-Jung Wu, Yun Chang, Tak Wah Wong, Jia-Horung Hung, Huai-Wen Hsu, I-Son Ng, and Nan Yao Lee
- Subjects
medicine.medical_specialty ,medicine.drug_class ,medicine.medical_treatment ,Antibiotics ,Pharmaceutical Science ,Photodynamic therapy ,Drug resistance ,Review ,Keratitis ,rose bengal ,drug-resistance ,Pharmacy and materia medica ,Cornea ,medicine ,Fungal keratitis ,Corneal epithelium ,business.industry ,fungal infection ,medicine.disease ,Dermatology ,eye diseases ,flavin mononucleotide ,Clinical trial ,RS1-441 ,medicine.anatomical_structure ,keratitis ,photodynamic therapy ,drug delivery ,candida ,business ,collagen cross-linking - Abstract
Fungal keratitis is a serious clinical infection on the cornea caused by fungi and is one of the leading causes of blindness in Asian countries. The treatment options are currently limited to a few antifungal agents. With the increasing incidence of drug-resistant infections, many patients fail to respond to antibiotics. Riboflavin-mediated corneal crosslinking (similar to photodynamic therapy (PDT)) for corneal ectasia was approved in the US in the early 2000s. Current evidence suggests that PDT could have the potential to inhibit fungal biofilm formation and overcome drug resistance by using riboflavin and rose bengal as photosensitizers. However, only a few clinical trials have been initiated in anti-fungal keratitis PDT treatment. Moreover, the removal of the corneal epithelium and repeated application of riboflavin and rose bengal are required to improve drug penetration before and during PDT. Thus, an improvement in trans-corneal drug delivery is mandatory for a successful and efficient treatment. In this article, we review the studies published to date using PDT against fungal keratitis and aim to enhance the understanding and awareness of this research area. The potential of modifying photosensitizers using nanotechnology to improve the efficacy of PDT on fungal keratitis is also briefly reviewed.
- Published
- 2021
35. Successful treatment of giant Bowen's disease with photodynamic therapy and subsequent imiquimod in a case with chronic arsenic exposure
- Author
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Tzu-Kun Lo, Yu-Chen Chen, Wei-Ting Liu, and Tak-Wah Wong
- Subjects
Oncology ,Biophysics ,Pharmacology (medical) ,Dermatology - Published
- 2022
36. In situ photoimmunotherapy is ineffective in treating deeply invasive squamous cell carcinoma
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Tak-Wah Wong, Stephanie Tsao, and Julia Yu-Yun Lee
- Subjects
imiquimod ,invasion ,photodynamic ,photoimmunotherapy ,squamous cell carcinoma ,Dermatology ,RL1-803 - Abstract
In situ photoimmunotherapy (ISPI) can be a treatment option for selected cutaneous malignancies in patients who are not surgical candidates. We herein report the case of a large, ulcerating poorly differentiated squamous cell carcinoma (SCC) affecting the foot of an elderly woman with chronic arsenicosis. The tumor failed radiotherapy, intralesional methotrexate, and 5-aminolevulinic acid photodynamic therapy (PDT). Because the patient was reluctant to undergo amputation, the recurrent tumor was treated with ISPI using topical imiquimod application followed by PDT. Despite some initial improvement in the superficial part of the tumor, tumor invasion to the underlying bone was detected. This case illustrates the lack of efficacy of ISIP in treating a high-risk invasive SCC.
- Published
- 2014
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37. Effects of Blue-Light-Induced Free Radical Formation from Catechin Hydrate on the Inactivation of Acinetobacter baumannii, Including a Carbapenem-Resistant Strain
- Author
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Meei-Ju Yang, Yi-An Hung, Tak-Wah Wong, Nan-Yao Lee, Jeu-Ming P. Yuann, Shiuh-Tsuen Huang, Chun-Yi Wu, Iou-Zen Chen, and Ji-Yuan Liang
- Subjects
ascorbic acid ,blue light ,catechin ,CRAB ,inactivation ,Organic chemistry ,QD241-441 - Abstract
Catechin is a flavan-3-ol, a derivative of flavans, with four phenolic hydroxyl groups, which exhibits a wide range of physiological properties. Chromatographic analyses were employed to examine the effects of blue light irradiation on the changes of catechin hydrate in an alkaline condition. In particular, the detection of a superoxide anion radical (O2•−), a reactive oxygen species (ROS), and the inactivation of Acinetobacter baumannii (A. baumannii)—including a carbapenem-resistant A. baumannii (CRAB)—was investigated during the photoreaction of catechin hydrate. Following basification with blue light irradiation, the transparent solution of catechin hydrate turned yellowish, and a chromogenic catechin dimer was separated and identified as a proanthocyanidin. Adding ascorbic acid during the photolytic treatment of catechin hydrate decreased the dimer formation, suggesting that ascorbic acid can suppress the photosensitive oxidation of catechin. When catechin hydrate was irradiated by blue light in an alkaline solution, O2•− was produced via photosensitized oxidation, enhancing the inactivation of A. baumannii and CRAB. The present findings on the photon-induced oxidation of catechin hydrate provides a safe practice for the inactivation of environmental microorganisms.
- Published
- 2018
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38. An AC electrokinetics-based electrochemical aptasensor for the rapid detection of microRNA-155
- Author
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Hsien-Chang Chang, Nan Yao Lee, Tak Wah Wong, and Neil Adrian P. Ondevilla
- Subjects
Detection limit ,Immunoassay ,Analyte ,Chromatography ,Chemistry ,Aptamer ,Biomedical Engineering ,Biophysics ,Early detection ,Nucleic Acid Hybridization ,General Medicine ,Biosensing Techniques ,Electrochemical Techniques ,Aptamers, Nucleotide ,Serum samples ,Electrochemistry ,Rapid detection ,Electrokinetic phenomena ,MicroRNAs ,Limit of Detection ,Electroosmosis ,Biotechnology - Abstract
Traditional immunosensors are often limited by low sensitivity and long detection times, for they usually depend on passive diffusion-dominated transport of target analytes for the binding reaction with a bio-recognition element such as enzymes, antibodies, and aptamers. Numerous studies rely on electric field manipulation by using alternating current (AC) electrokinetics to enhance the hybridization rate and reduce the hybridization time for faster and more efficient detection. This study demonstrated a rapid electrochemical aptasensor integrated with an AC electroosmotic (ACEO) flow phenomenon for the enhanced target hybridization of microRNA-155 (miR-155). Optimization of the electrokinetic conditions for target collection resulted in a saturation point after 75 s. miR-155 was detected within the range of 1 aM–10 pM with a detection limit of 1 aM, which is 100 times lower and about 50 times faster compared with the conventional diffusion-dependent detection done for 1 hr. The detection was also done in spiked serum samples, and a concentration range within the required detection range was obtained. The highly sensitive and specific results allow for the rapid and real-time sensing of target biomarkers, which can be used for the early detection of infection.
- Published
- 2021
39. Pilot Study of Topical Delivery of Mono-L-aspartyl Chlorin e6 (NPe6): Implication of Topical NPe6-Photodynamic Therapy
- Author
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Tak-Wah Wong, Katsuo Aizawa, Ilyar Sheyhedin, ChiMenGul Wushur, and Harubumi Kato
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
ABSTRACT: Photodynamic therapy (PDT) is an evolving cancer treatment with promising results in treating malignant tumors by photoactivation of a photosensitizer with a specific wavelength. The second generation photosensitizer mono-L-aspartyl chlorin e6 (NPe6) was reported to have significant efficacy in killing cancer cells in vitro and in vivo. Though topical application might yield a higher local concentration and less systemic side effect, no data concerning topical absorption of NPe6 is available even though the drug has already been used in clinical trial for several years. To evaluate the possibility of topical delivery of NPe6 via an animal model, escalated concentrations of NPe6 were applied to BALB/c mouse skin for a different time periods after barrier disruption with tape stripping. Since NPe6 fluorescence intensity and drug concentration in tissue was well correlated, we evaluated drug penetration depth with frozen sections of treated and non-treated skin under a fluorescence microscope. An on-line fluorescence imaging system was used to monitor the NPe6 fluorescence kinetics in the skin. The fluorescence microscope confirmed successful topical delivery of NPe6 in mouse skin with or even without barrier disruption. Orange to red NPe6 fluorescence appeared at the epidermis, dermis, and even the muscular layer when using 10 mg/ml NPe6 application. The fluorescence intensity peaked at 1 h and revealed a dose-dependent response pattern. NPe6 treated versus non-treated skin showed a statistically significant difference by Student’s t-test (P
- Published
- 2003
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40. Regeneration of rete ridges in Lanyu pig ( Sus scrofa ): Insights for human skin wound healing
- Author
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Chia Ching Wu, Yuan Yu Hsueh, Chein Hong Lin, Tak Wah Wong, Michael W. Hughes, Cheng-Ming Chuong, Po Yuan Chiu, and Shyh Jou Shieh
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Sus scrofa ,Population ,Human skin ,Dermatology ,Biochemistry ,Neogenesis ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,education ,Molecular Biology ,Skin ,Wound Healing ,education.field_of_study ,integumentary system ,biology ,Regeneration (biology) ,Papillary dermis ,biology.organism_classification ,Hair follicle ,030104 developmental biology ,medicine.anatomical_structure ,Models, Animal ,biology.protein ,Wound healing ,Elastin - Abstract
Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full-thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full- and partial-thickness wounds were generated on the dorsum. Full-thickness wounds at post-wound day 57 exhibit severe scar with no signs of wound-induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14+ cells increased while K10+ , p63+ and PCNA+ cells decreased compared to unwounded tissue. Epithelial β-catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full-thickness wounding, but incompletely regenerate after partial-thickness wounding. An alkaline phosphatase (ALP)+ cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5+ /K10- /PCNA- /ALP+ epithelial cells are absent after full-thickness wounding but reappear after partial-thickness wounding, before invagination of new rete ridges. In summary, full-thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial-thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.
- Published
- 2019
41. Corrigendum to 'Taiwanese dermatological association consensus statement on management of psoriasis' [Dermatol Sin 35 (2017) 66–77]
- Author
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Tsen-Fang Tsai, Chih-Hung Lee, Yu-Huei Huang, Ching-Chi Chi, Yun-Ting Chang, Tak-Wah Wong, Chih-Hsun Yang, and Li-Fang Wang
- Subjects
Dermatology ,RL1-803 - Published
- 2017
- Full Text
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42. Infusing problem solving skills into computer lessons.
- Author
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Yiu-chi Lai and Tak-wah Wong
- Published
- 2007
- Full Text
- View/download PDF
43. Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence
- Author
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Lewis Krey, S. Talebian, Tuan Phi Nguyen, Herbert Lepor, Tung-Tien Sun, H.C. Chang, Feng-Xia Liang, Pei Jung Chung, Ge Zhou, Xue-Ru Wu, Yuan Wei, James A. Grifo, Antonio Garcia-España, Robert DeSalle, Sang Yong Kim, Tak Wah Wong, Fang Ming Deng, David L. Keefe, Ellen Shapiro, Javier U. Chicote, and Yi Liao
- Subjects
Male ,0301 basic medicine ,Cell type ,Litter Size ,Genetic Speciation ,Zygote ,Cellular differentiation ,Parthenogenesis ,Xenopus ,Biology ,Proto-Oncogene Proteins c-fyn ,urologic and male genital diseases ,Tetraspanin 29 ,Mice ,Xenopus laevis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Testis ,Uroplakins ,Animals ,Phosphorylation ,Urothelium ,Molecular Biology ,Phylogeny ,Mice, Knockout ,Ovary ,Cell Differentiation ,Tyrosine phosphorylation ,Articles ,Cell Biology ,biology.organism_classification ,Cell biology ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Membrane Trafficking ,Cytoplasm ,Fertilization ,030220 oncology & carcinogenesis ,Oocytes ,Female ,Signal transduction ,Signal Transduction - Abstract
Uroplakin (UP) tetraspanins and their associated proteins are major mammalian urothelial differentiation products that form unique two-dimensional crystals of 16-nm particles (“urothelial plaques”) covering the apical urothelial surface. Although uroplakins are highly expressed only in mammalian urothelium and are often referred to as being urothelium specific, they are also expressed in several mouse nonurothelial cell types in stomach, kidney, prostate, epididymis, testis/sperms, and ovary/oocytes. In oocytes, uroplakins colocalize with CD9 on cell-surface and multivesicular body-derived exosomes, and the cytoplasmic tail of UPIIIa undergoes a conserved fertilization-dependent, Fyn-mediated tyrosine phosphorylation that also occurs in Xenopus laevis eggs. Uroplakin knockout and antibody blocking reduce mouse eggs’ fertilization rate in in vitro fertilization assays, and UPII/IIIa double-knockout mice have a smaller litter size. Phylogenetic analyses showed that uroplakin sequences underwent significant mammal-specific changes. These results suggest that, by mediating signal transduction and modulating membrane stability that do not require two-dimensional-crystal formation, uroplakins can perform conserved and more ancestral fertilization functions in mouse and frog eggs. Uroplakins acquired the ability to form two-dimensional-crystalline plaques during mammalian divergence, enabling them to perform additional functions, including umbrella cell enlargement and the formation of permeability and mechanical barriers, to protect/modify the apical surface of the modern-day mammalian urothelium.
- Published
- 2018
44. Health-related quality of life among patients with moderate-to-severe plaque psoriasis in Taiwan
- Author
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Ji Chen Ho, Hsiu Cheng Hsu, Yu-Huei Huang, Tak Wah Wong, Pa Fan Hsiao, Hamm Ming Sheu, Ching-Chi Chi, Woan Ruoh Lee, Cheng Che Lan, Yi-Ju Chen, Gong Yau Chu, Kuo Chia Yang, Tsen-Fang Tsai, Rosaline Chung-Yee Hui, and Yang Chih Lin
- Subjects
Plaque psoriasis ,Body surface area ,Health related quality of life ,medicine.medical_specialty ,business.industry ,Standard treatment ,Dermatology ,Dermatology Life Quality Index ,lcsh:RL1-803 ,medicine.disease ,humanities ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Quality of life ,EQ-5D ,030220 oncology & carcinogenesis ,Internal medicine ,Psoriasis ,lcsh:Dermatology ,Medicine ,business - Abstract
Background/Objectives: Plaque psoriasis is a debilitating condition that significantly affects patient well-being. Limited data are available regarding the effect of psoriasis and treatment on health-related quality of life (HRQoL) and work ability among Taiwanese patients.To document and compare HRQoL, treatment satisfaction, and work disability among Taiwanese patients with current and past moderate-to-severe plaque psoriasis. Methods: This was a multicenter, non-interventional, cross-sectional study of adult patients with moderate-to-severe plaque psoriasis. During a single clinic visit, each patient was assessed for body surface area (BSA) involvement, Psoriasis Area Severity Index (PASI), Dermatology Life Quality Index (DLQI), Euro Quality of Life-5 Dimensions (EQ-5D), 10-level satisfaction scale for psoriasis treatment, and Working Productivity and Activity Impairment (WPAI). Multivariate regression was used to identify factors associated with HRQoL and work disability. Results: A total of 305 patients were included within the analysis. The mean PASI score was 11.83, and the mean BSA involvement was 20.90%. The mean EQ-5D score was 65.68 and the mean DLQI score was 12.55. Fewer than half of patients (45.68%) indicated they were satisfied with the standard therapy they were currently receiving. Among employed patients, the mean reduction in on-the-job effectiveness was 32.09% and the mean reduction in overall productivity was 33.48%. The regression analysis indicated that patients with more severe psoriasis defined by PASI scores show a greater impact in quality of life and impairment in work disability; and that patients who were satisfied with current standard treatment had a better quality of life. Conclusion: The effect of psoriasis on HRQoL among patients with psoriasis in Taiwan is substantial, with fewer than half of patients reporting satisfaction with therapeutic options. Keywords: DLQI, EQ-5D, Health-related quality of life, Psoriasis, WPAI
- Published
- 2018
45. Spontaneous resolution of recalcitrant generalized prurigo nodularis after resection of an ampulla of Vater tumor
- Author
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Yun-Ting Su, Julia Yu-Yun Lee, and Tak-Wah Wong
- Subjects
Dermatology ,RL1-803 - Published
- 2010
- Full Text
- View/download PDF
46. Real-world efficacy of biological agents in moderate-to-severe plaque psoriasis: An analysis of 75 patients in Taiwan
- Author
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Hamm Ming Sheu, Sheau Chiou Chao, Chao Kai Hsu, Cheng Han Liu, Chao Chun Yang, Yi Ting Huang, Chaw Ning Lee, Tak Wah Wong, Yu Chen Chen, and Edward C. Lai
- Subjects
Male ,Economics ,Cancer Treatment ,Social Sciences ,Cardiovascular Medicine ,Severity of Illness Index ,Etanercept ,Geographical Locations ,Biological Factors ,Medical Conditions ,Medicine and Health Sciences ,Medicine ,skin and connective tissue diseases ,Multidisciplinary ,Pharmaceutics ,Middle Aged ,humanities ,Cardiovascular Therapy ,Treatment Outcome ,Oncology ,Research Design ,Cardiovascular Diseases ,Female ,Ustekinumab ,medicine.drug ,Research Article ,musculoskeletal diseases ,Adult ,medicine.medical_specialty ,Asia ,Adolescent ,Clinical Research Design ,Science ,Immunology ,Taiwan ,Cardiology ,Research and Analysis Methods ,Antibodies, Monoclonal, Humanized ,Autoimmune Diseases ,Young Adult ,Health Economics ,Drug Therapy ,Psoriasis Area and Severity Index ,Psoriasis ,Internal medicine ,Adalimumab ,Humans ,Adverse effect ,Aged ,Retrospective Studies ,business.industry ,Biology and Life Sciences ,medicine.disease ,Health Care ,People and Places ,Secukinumab ,Clinical Immunology ,Adverse Events ,Clinical Medicine ,business ,Body mass index ,Health Insurance - Abstract
Background Real-world clinical data on psoriasis patients receiving different biological agents is needed, especially in Asian populations. Objectives Our aim is to compare and analyze the efficacy and safety profile of four biological agents (etanercept, adalimumab, ustekinumab and secukinumab) in a real-world setting in Taiwan. Methods We retrospectively analyzed the clinical data of all patients with moderate-to-severe plaque psoriasis (Psoriasis Area and Severity Index (PASI) ≥ 10) who received etanercept, adalimumab, ustekinumab or secukinumab between January 2011 and December 2018 in a tertiary hospital in Taiwan. Results A total of 119 treatment episodes in 75 patients were included in this study. Ustekinumab was used in 49 treatment episodes, followed by secukinumab in 46 treatment episodes, adalimumab in 14 treatment episodes and etanercept in 10 treatment episodes. The proportion of the biologic-naïve was highest in etanercept (100%) and lowest in secukinumab (23.9%). The PASI-75, -90 and -100 were the highest in secukinumab (91.3%, 82.6%, 41.3%, respectively), followed by ustekinumab (79.6%, 44.9%, 16.3%), adalimumab (64.3%, 28.6%, 7.1%) and etanercept (50.0%, 30.0%, 0%). The rate of adverse events that required treatment was highest for secukinumab (15.2%), followed by adalimumab (14.3%), ustekinumab (8.2%), and etanercept (0%), including 4 cases of infections, 2 cases of cardiovascular diseases and 4 cases of cancers. Conclusions This real world data showed differential efficacy and safety of the four biological agents.
- Published
- 2020
47. Optimize General Anesthesia for a Dystrophic Epidermolysis Bullosa Patient That Cannot Be Intubated
- Author
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Wan-Ting, Chia, Yen-Chin, Liu, and Tak-Wah, Wong
- Subjects
Cicatrix ,Blister ,Humans ,Female ,Anesthesia, General ,Epidermolysis Bullosa Dystrophica ,Skin - Abstract
Dystrophic epidermolysis bullosa (DEB) is a rare genetic skin disease characterized by blisters and ulcers on the skin and mucosa after minor friction. The risk of invasive squamous cell carcinoma on the unhealed ulcers increases with age. Tracheal intubation during general anesthesia may induce tracheal stricture due to blister formation and/or scarring in DEB patients and cause severe airway obstruction. There is no consensus for handling DEB patients' fragile mucosa and skin during general anesthesia. We report an adult DEB patient who received two operations under different general anesthesia methods. The experience from this particular patient and her response to anesthesia may provide a satisfactory guide to avoid complications and improve the outcome for DEB patients receiving general anesthesia.
- Published
- 2020
48. Enhancing wound healing in recalcitrant leg ulcers with aminolevulinic acid-mediated antimicrobial photodynamic therapy
- Author
-
Julia Yu-Yun Lee, Ming Hsien Lin, Shin Chen Pan, and Tak Wah Wong
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,030303 biophysics ,Biophysics ,Photodynamic therapy ,Dermatology ,Gastroenterology ,Chronic ulcers ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Refractory ,Anti-Infective Agents ,Internal medicine ,Diabetes mellitus ,polycyclic compounds ,medicine ,Humans ,Pharmacology (medical) ,Keratinocyte migration ,0303 health sciences ,Wound Healing ,Photosensitizing Agents ,business.industry ,Leg Ulcer ,Aminolevulinic Acid ,Skin ulcer ,Antimicrobial ,medicine.disease ,digestive system diseases ,eye diseases ,Oncology ,Photochemotherapy ,medicine.symptom ,business ,Wound healing ,therapeutics - Abstract
Chronic leg ulcers effect millions of people around the world. It is imperative to search for effective treatments for such challenging ulcers. We report the success of aminolevulinic acid-mediated antimicrobial photodynamic therapy (A-PDT) to enhance wound healing of chronic ulcers for 3 patients who were refractory to conventional treatments. These ulcers healed after one to three sessions of A-PDT and there was no recurrence for more than 29 months. Interestingly, no bacteria were isolated from the ulcers after A-PDT treatment. In vitro, A-PDT also inactivated all bacteria isolated from the patients. A-PDT conditioned medium containing IL-6 enhanced keratinocyte migration. The results suggest in addition to bactericidal effects, A-PDT also alters the wound microenvironment. A-PDT may be an effective treatment for patients with recalcitrant infected ulcers.
- Published
- 2020
49. Imiquimod Accelerated Antitumor Response by Targeting Lysosome Adaptation in Skin Cancer Cells
- Author
-
Cheng Chung Chang, Jeng-Jer Shieh, Yi Ju Chen, Shu Hao Chang, Zheng Yi Li, Sin Ting Wang, Tak Wah Wong, Zi Lun Lai, Shi Wei Huang, Kai Cheng Chuang, Chun Ying Wu, and Jun Kai Kao
- Subjects
0301 basic medicine ,Cathepsin D ,Antineoplastic Agents ,Apoptosis ,Dermatology ,Biochemistry ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Stomach Neoplasms ,Cyclosporin a ,Lysosome ,medicine ,Animals ,Humans ,Calcium Signaling ,Molecular Biology ,Mice, Knockout ,Imiquimod ,Chemistry ,Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ,Calcineurin ,Cell Biology ,Xenograft Model Antitumor Assays ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer cell ,TFEB ,Keratinocyte ,Lysosomes ,Reactive Oxygen Species ,Intracellular - Abstract
Lysosomal adaptation is a cellular physiological process in which the number and function of lysosomes are regulated at the transcriptional and post-transcriptional levels in response to extracellular and/or intracellular cues or lysosomal damage. Imiquimod (IMQ), a synthetic toll-like receptor 7 ligand with hydrophobic and weak basic properties, exhibits both antitumor and antiviral activity against various skin malignancies as a clinical treatment. Interestingly, IMQ has been suggested to be highly concentrated in the lysosomes of plasmacytoid dendritic cells, indicating that IMQ could modulate lysosome function after sequestration in the lysosome. In this study, we found that IMQ not only induced lysosomal membrane permeabilization and dysfunction but also increased lysosome biogenesis to achieve lysosomal adaptation in cancer cells. IMQ-induced ROS production but not lysosomal sequestration of IMQ was the major cause of lysosomal adaptation. Moreover, IMQ-induced lysosomal adaptation occurred through lysosomal calcium ion release and activation of the calcineurin/TFEB axis to promote lysosome biogenesis. Finally, depletion of TFEB sensitized skin cancer cells to IMQ-induced apoptosis in vitro and in vivo. In summary, a disruption of lysosomal adaptation might represent a therapeutic strategy for synergistically enhancing the cytotoxicity of IMQ in skin cancer cells.
- Published
- 2020
50. A clinicopathological study of perianal paget disease: A single center-based cohort study and literature review
- Author
-
JuliaYu-Yun Lee, Ping-Chen Hou, Chaw-Ning Lee, Tak-Wah Wong, Tzu-Chien Hsu, and Cheng-Lin Wu
- Subjects
Dermatology - Published
- 2022
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