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Manganese (Mn) and aluminum (Al) toxicities are serious edaphic limitations to crop production in acidic soils. Excess Mn can be countered using a stress-adapted soil microbiota that establish symbiotic relationships with native plants. The arbuscular mycorrhizal fungi (AMF) associated with Lolium rigidum L. develop extraradical mycelia (ERM) that quickly colonize wheat and lead to greater shoot growth by promoting stress-evading mechanisms that are not yet completely explained. In the present study, wheat growth was assessed after 3 weeks on disturbed and undisturbed (intact ERM) acidic soil where the native non-mycotrophic Silene gallica L. or strongly mycotrophic L. rigidum were previously developed. The physiological and biochemical mechanisms responsible for increased growth were analyzed by assessing wheat leaf chlorophyll content, photosystem II quantum yield and performance index, enzymatic activity of ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (GPX), superoxide dismutase (SOD) and contents and subcellular localization of Mn, Mg, Si and K. The soil from native plants had a beneficial effect on shoot weight and chlorophyll levels. The highest benefits were obtained for wheat grown in soil with intact ERM associated with L. rigidum. In this condition, where earlier mycorrhization was favored, the Mn content decreased, alongside the content of Si, while the Mg/Mn ratio increased. Mn was redirected to the apoplast, while Si was redirected to the symplast. The activity of APX, GPX and SOD increased, probably due to increased metabolic growth (higher shoot weight and chlorophyll content). Understanding the mechanisms induced by native AMF responsible for increasing wheat performance can contribute to the establishment of sustainable approaches for crop production in acidic soils with Mn toxicity. The use of native plant AMF developers can improve the sustainable use of natural resources in the scope of greener agricultural practices.

Background: Few studies have compared COVID-19 patients from different waves. This study aims to conduct a clinical and morphological analysis of patients who died from COVID-19 during four waves. Methods: The study involved 276 patients who died from COVID-19 during four waves, including 77 patients in the first wave, 119 patients in the second wave, and 78 patients in the third wave. We performed a histological examination of myocardium samples from autopsies and additionally analyzed the samples by PCR. We conducted immunohistochemistry of the myocardium for 21 samples using antibodies against CD3, CD45, CD8, CD68, CD34, Ang1, VWF, VEGF, HLA-DR, MHC1, C1q, enteroviral VP1, and SARS-CoV-2 spike protein. We also did immunofluorescent staining of three myocardial specimens using VP1/SARS-CoV-2 antibody cocktails. Further, we ran RT-ddPCR analysis for 14 RNA samples extracted from paraffin-embedded myocardium. Electron microscopic studies of the myocardium were also performed for two samples from the fourth wave. Results: Among the 276 cases, active myocarditis was diagnosed in 5% (15/276). Of these cases, 86% of samples expressed VP1, and individual cells contained SARS-CoV-2 spike protein in 22%. Immunofluorescence confirmed the co-localization of VP1 and SARS-CoV-2 spike proteins. ddPCR did not confidently detect SARS-CoV-2 RNA in the myocardium in any myocarditis cases. However, the myocardium sample from wave IV detected a sub-threshold signal of SARS-CoV-2 by qPCR, but myocarditis in this patient was not confirmed. Electron microscopy showed several single particles similar to SARS-CoV-2 virions on the surface of the endothelium of myocardial vessels. A comparison of the cardiovascular complication incidence between three waves revealed that the incidence of hemorrhage (48 vs. 24 vs. 17%), myocardial necrosis (18 vs. 11 vs. 4%), blood clots in the intramural arteries (12 vs. 7 vs. 0%), and myocarditis (19 vs. 1 vs. 6%) decreased over time, and CD8-T-killers appeared. Immunohistochemistry confirmed the presence of endotheliitis in all 21 studied cases. Conclusions: This study compared myocardial damage in patients who died during three COVID-19 waves and showed a decrease in the incidence of endotheliitis complications (thrombosis, hemorrhage, necrosis) and myocarditis over time. However, the connection between myocarditis and SARS-CoV-2 infection remains unproven.

In the current agronomic context, the adoption of alternative forms of soil management is essential to increase crop yield. Agricultural sustainability requires practices that generate positive impacts and promote an increase in microbiome diversity as a tool to overcome adverse environmental conditions. An important ally is the indigenous arbuscular mycorrhizal fungi (AMF) that can improve plant growth and provide protection against abiotic stress such as metal toxicity. In a greenhouse experiment, this work studied the effect of wheat growth on several parameters of biological activity and functional microbiome in relation to wheat antecedent plant mycotrophy and soil disturbance under Mn stress. When the wheat was planted after highly mycotrophic plants and the soil was not previously disturbed, the results showed a 60% increase in wheat arbuscular colonization and a 2.5-fold increase in dry weight along with higher values of photosynthetic parameters and dehydrogenase activity. Conversely, soil disturbance before wheat planting increased the β-glucosidase activity and the count of manganese oxidizers, irrespectively of antecedent plant, and decreased drastically the wheat dry weight, the AMF colonization and the chlorophyll content compared to the undisturbed treatment. These findings suggest that not only the wheat growth but also the soil functional microbiome associated is affected by the antecedent type of plant and previous soil disturbance imposed. In addition, the improvement in wheat dry weight despite Mn toxicity may rely on shifts in biological activity associated to a well-established and intact ERM early developed in the soil.

The study aimed to identify puerperal knowledge about reproductive planning. This is a descriptive and exploratory study, with a qualitative approach and focus on Dorothea Orem’s Theory of Self-Care. Data collection took place in family health units of a municipality in the northern region of Mato Grosso, between June and July 2016, together with 17 puerperas, through a semi-structured interview. Data analysis was used to analyze the data. It was observed that the reproductive planning is not being carried out integrally, as well as the finding of absence of gestational follow-up by the nurse during the pregnancy-puerperal process. This professional behavior tends to represent problems in adhering to self-care practices at this stage and to induce women and their partners into unsafe attitudes. Finally, it was verified that the reproductive planning is little known by the women of the study and there is a strong relation with the inertia of the professionals. Thus, new studies must be carried out, verifying the relationships of the professionals with the new family configurations, liberating from the hegemonic conception of birth control and sterility, so present in the healthcare environments of the Brazilian health services.

Introduction: Mesenchymal stromal cells (MSCs) gained attention for their anti-inflammatory and trophic properties, with musculoskeletal diseases and osteoarthritis (OA) being among the most studied conditions. Alongside cells, their released factors and extracellular vesicles (EVs), overall termed “secretome”, are actively sifted being envisioned as the main therapeutic actors. In addition to standard supplementation given by foetal bovine serum (FBS) or human platelet lysate (hPL), new good manufacturing practice (GMP)-compliant serum/xeno (S/X)-free media formulations have been proposed, although their influence on MSCs phenotype and potential is scarcely described. The aim of this study is therefore to evaluate, in the OA context, the differences in secretome composition and potential after adipose-MSCs (ASCs) cultivation in both standard (FBS and hPL) and two next generation (S/X) GMP-ready supplements. Methods: Immunophenotype and secretory ability at soluble protein and EV-related levels, including embedded miRNAs, were analysed in the secretomes by means of flow cytometry, nanoparticle tracking analysis, high throughput ELISA and qRT-PCR arrays. Secretomes effect was tested in in vitro models of chondrocytes, lymphocytes and monocytes to mimic the OA microenvironment. Results: Within a conserved molecular signature, a divergent fingerprint emerged for ASCs' secretomes collected after expansion in standard FBS/hPL or next-generation S/X formulations. Regarding soluble factors, a less protective feature for those in the secretome collected after ASCs were cultured in S/X media emerged. Moreover, the overall message for EV-miRNAs was characterized by a preponderance of protective signals in FBS and hPL conditions in a context of general safeguard given by ASCs released molecules. This dichotomy was reflected on secretomes’ potential in vitro, with expansion in hPL resulting in the most effective secretome for chondrocytes and in FBS for immune cells. Conclusions: These data open the question about the implications from using new media for MSCs expansion for clinical application. Although the undeniable advantages for GMP compliant processes, this study results suggest that new media formulations would deserve a deep characterization to drive the choice of the most effective one tailored to each specific application.

The Power Sector is undergoing a rapid technological change with respect to implementation of low carbon technologies. The IEA Energy Outlook 2017 shows that the investments in Renewables for the first time are equal to those on the fossil sources. It is likely that the conventional gas turbines and internal combustion engines will need to be integrated in systems employing biofuels and/or CCUS (Carbon Capture Usage and Storage). Also, the European Union is moving rapidly towards low carbon technologies (i.e. Energy Efficiency, Smart Grids, Renewables and CCUS), see the Energy Union Strategy. Currently 28% of the installed power capacity in Europe is based on natural gas plants. Gas-based power capacity has reached 418 GW in 2016 and is likely to continue to grow in the future. To efficiently capture the carbon dioxide emissions generated by the combustion of natural gas in the combustion chamber a possible solution could be to adopt new combustion processes, like Chemical Looping Combustion. The combination of CLC and GTs can decrease the efficiency of a combined cycle power plant from 60% to about 40.34%. These performances influence costs and environmental burdens and this is also the same for oxyfuel combustion, which is a competing technology to realize CCS. This paper, starting from literature mass and energy balances of a conventional combined cycle, a combined cycle coupled with chemical looping combustor and a combined cycle coupled with oxyfuel combustion, calculates the reduction of CO2 emissions which can be achieved during the whole life cycle of the power plant and then identifies the value of the carbon credit which is needed to have an interesting payback period for such kind of investment.

Objetivo: conhecer os acidentes com pré-escolares atendidos em uma Estratégia Saúde da Família. Método: documental com abordagem quantitativa. Coletando os dados no livro de procedimentos da unidade de saúde entre 2010 a 2013. Na análise foi utilizado a estatística descritiva. Resultados: verificou-se 33% (n=13) correspondendo a ferimentos na região da cabeça/face, sendo 100% (n=39) atendidos pelo enfermeiro. Após atendimento, 46% (n=18) foram encaminhados para suas residências. Os professores corresponderam a 26% (n=10) dos acompanhantes. Conclusões: além do investimento em políticas públicas que respondam ao desafio da redução dos acidentes, é necessário, também, que os profissionais de saúde, educadores e cuidadores sejam proativos e capazes de reconhecer esses riscos, propondo soluções em tempo hábil para evitar acidentes, sequelas e óbitos.

Soil microorganisms play a significant role in the dynamic regulation of organic matter in soils. To assess the influence of agricultural practices on soil functional profiling, we examined the effect of soil disturbance and plant sequence with different levels of mycotrophy on wheat microbiomes metabolism. Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ Ecoplates. The results of average well color development (AWCD) showed that the degree of mycotrophy of preceding crop and soil disturbance affected the soil microbiome, although no impact on Shannon Evenness Index was observed during the experiment. The Shannon–Wiener Diversity Index showed variations among the different preceding plants, but not in wheat analysis. The pattern of the C sources metabolism also changed differentially regarding plant type and soil disturbance during the experiment, being also different within the highly mycotrophic plants (legume and grass). In the legume, an increase in the metabolism of amine/amides and phenolic acids was observed, whilst in the grass, an increase in the metabolism of phosphate-carbons (P carbon) and carbohydrates was more evident. Principal component analysis showed that a grouping in the distinct phases of the experiment correlated with the widening of the metabolism of amino acids, carboxylic acids, and carbohydrates. The results indicate that soil functional community structure reflects soil agricultural practice conditions. Previous plant types and soil disturbance impacted the soil microbiome metabolic response (AWCD) in wheat, generating different patterns of carbon metabolism related to previous plant mycotrophy.

Abstract: Objective This study sought to investigate associations between illness perception, adherence to antiretroviral medication, and levels of anxiety and depression in individuals diagnosed with HIV/AIDS. Method One hundred and one patients receiving outpatient care at a university hospital completed the Brief Illness Perception Questionnaire, the Questionnaire for the Assessment of Adherence to Antiretroviral Therapy, and the Hospital Anxiety and Depression Scale. Results Through statistical analyses, using Fisher's exact and Chi-square tests, a statistically significant positive association (p < 0.001) was found between scores of illness perception and anxiety and depression, suggesting that the greater the perception of threat imposed by the disease, the greater the frequency of symptoms of anxiety and depression. Negative and significant associations were also found between adherence to medical prescriptions and perceived threat of living with HIV/AIDS (p < 0.001) and levels of anxiety (p < 0.043). Conclusion Research on psychological variables in chronically ill individuals is necessary to support assessments and interventions in the field.

Ciguatera poisoning (CP) is caused by the consumption of marine products contaminated with ciguatoxins (CTXs) produced by dinoflagellates of the genus Gambierdiscus. Analytical methods for CTXs, involving the extraction/purification of trace quantities of CTXs from complex matrices, are numerous in the literature. However, little information on their effectiveness for nonpolar CTXs is available, yet these congeners, contributing to the risk of CP, are required for the establishment of effective food safety monitoring programs. An evaluation of six extraction/purification protocols, performed with CTX3C spiked on fish flesh and a neuroblastoma cell-based assay (CBA-N2a), revealed recoveries from 6 to 45%. This led to the development of an optimized 3-day protocol designed for a large number of samples, with CTX1B and CTX3C eluting in a single fraction and showing recoveries of 73% and 70%, respectively. In addition, a reduction in adverse matrix effects in the CBA-N2a analyses was demonstrated with naturally contaminated specimens, increasing the sensitivity of the method, which now meets the very low guidance level recommended by international agencies. However, efforts are still required to reduce the signal suppression observed in LC-MS/MS analysis. This optimized protocol contributes to the technological advancement of detection methods, promoting food safety and improving CP risk assessment in marine products.