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Gene expression (GE) analysis of a radio-sensitive gene set (FDXR, DDB2, WNT3, POU2AF1) has been introduced in the last decade as an early and high-throughput prediction tool of later developing acute hematologic radiation syndrome (H-ARS) severity. The use of special tubes for RNA extraction from peripheral whole blood (PAXgene) represent an established standard in GE studies, although uncommonly used in clinics and not immediately available in the quantities needed in radiological/nuclear (R/N) incidents. On the other hand, EDTA blood tubes are widely utilized in clinical practice. Using blood samples from eleven healthy donors, we investigated GE changes associated with delayed processing of EDTA tubes up to 4 h at room temperature (RT) after venipuncture (simulating delays caused by daily clinical routine), followed by a subsequent transport time of 24 h at RT, 4 °C, and −20 °C. Differential gene expression (DGE) of the target genes was further examined after X-irradiation with 0 Gy and 4 Gy under optimal transport conditions. No significant changes in DGE were observed when storing EDTA whole blood samples up to 4 h at RT and subsequently kept at 4 °C for 24 h which is in line with expected DGE. However, other storage conditions, such as −20 °C or RT, decreased RNA quality and/or (significantly) caused changes in DGE exceeding the known methodological variance of the qRT-PCR. Our data indicate that the use of EDTA whole blood tubes for GE-based H-ARS severity prediction is comparable to the quality of PAXgene tubes, when processed ≤ 4 h after venipuncture and the sample is transported within 24 hours at 4 °C. [ABSTRACT FROM AUTHOR]

Vector or reservoir species of five mollusc diseases listed in the Animal Health Law were identified, based on evidence generated through an extensive literature review, to support a possible updating of Regulation (EU) 2018/1882. Mollusc species on or in which Mikrocytos mackini, Perkinsus marinus, Bonamia exitiosa, Bonamia ostreae and Marteilia refringens were detected, in the field or during experiments, were classified as reservoir species with different levels of certainty depending on the diagnostic tests used. Where experimental evidence indicated transmission of the pathogen from a studied species to another known susceptible species, this studied species was classified as a vector species. Although the quantification of the risk of spread of the pathogens by the vectors or reservoir species was not part of the terms of reference, such risks do exist for the vector species, since transmission from infected vector species to susceptible species was proven. Where evidence for transmission from infected molluscs was not found, these were defined as reservoir. Nonetheless, the risk of the spread of the pathogens from infected reservoir species cannot be excluded. Evidence identifying conditions that may prevent transmission by vectors or reservoir mollusc species during transport was collected from scientific literature. It was concluded that it is very likely to almost certain (90–100%) that M. mackini, P. marinus, B. exitiosaB. ostreae and M. refringens will remain infective at any possible transport condition. Therefore, vector or reservoir species that may have been exposed to these pathogens in an affected area in the wild or at aquaculture establishments or through contaminated water supply can possibly transmit these pathogens. For transmission of M. refringens, the presence of an intermediate host, a copepod, is necessary. This publication is linked to the following EFSA Journal articles: http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2023.8172/full, http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2023.8174/full This publication is linked to the following EFSA Supporting Publications articles: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8122/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8123/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8124/full [ABSTRACT FROM AUTHOR]

Vector or reservoir species of three diseases of crustaceans listed in the Animal Health Law were identified based on evidence generated through an extensive literature review, to support a possible updating of Regulation (EU) 2018/1882. Crustacean species on or in which Taura syndrome virus (TSV), Yellow head virus (YHV) or White spot syndrome virus (WSSV) were identified, in the field or during experiments, were classified as reservoir species with different levels of certainty depending on the diagnostic tests used. Where experimental evidence indicated transmission of the pathogen from a studied species to another known susceptible species, the studied species was classified as vector species. Although the quantification of the risk of spread of the pathogens by the vectors or reservoir species was not part of the terms of reference, such risks do exist for the vector species, since transmission from infected vector species to susceptible species was proven. Where evidence for transmission from infected crustaceans was not found, these were defined as reservoirs. Nonetheless, the risk of the spread of the pathogens from infected reservoir species cannot be excluded. Evidence identifying conditions that may prevent transmission by vectors during transport was collected from scientific literature. It was concluded that it is very likely to almost certain (90–100%) that WSSV, TSV and YHV will remain infective at any possible transport condition. Therefore, vector or reservoir species that may have been exposed to these pathogens in an affected area in the wild or aquaculture establishments or by water supply can possibly transmit WSSV, TSV and YHV. This publication is linked to the following EFSA Journal articles: http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2023.8173/full, http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2023.8174/full This publication is linked to the following EFSA Supporting Publications articles: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8122/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8123/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8124/full [ABSTRACT FROM AUTHOR]

The aim of this study was to establish the impact of air transport on blood samples packaged with and without cooling elements and effect of outdoor temperature on sample quality. Venous samples from 38 blood donors in winter and 36 in summer were tested for hemolysis and complete blood count. One tube per subject was kept in controlled conditions at +4 °C. Two sets of tubes were sent by plane from Zagreb to Brussels, one with and one without cooling elements, and another two sets were sent to London following the same principle. Packages with cooling elements were stored in controlled warehousing conditions at airports (+2 °C to +8 °C), whereas packages without cooling elements were stored in ambient warehouse conditions. Data loggers were used for temperature monitoring. Our research revealed statistically significant differences in several hematologic parameters when comparing the samples stored in controlled laboratory conditions and those transported by plane. These differences were more pronounced in the samples transported during the summer. Transport conditions without cooling elements had additional negative impact on the sample quality. Transport of samples using cooling elements and controlled warehousing conditions at airports are sometimes not sufficient to maintain laboratory storage conditions.

Sanitary standards for drinking milk are often affected by unsuitable storage and transport conditions. The composition of milk is sensitive to external factors. The effect of physico-chemical factors on the bacteriology of milk during its storage phase and its transport phase is analyzed by artificial neural networks. Also, a strong acidity is unfavorable to their multiplication. Add to this, the hygiene of the container and the personnel as well as the shelf life play a big role. In order to maintain the milk in optimal conditions, it is necessary to control the storage and transport chain. This study supports the parameters that influence the process. An artificial neural network system is proposed in this analysis. The system input variables represent the factors characterizing these conditions. The output variable is expressed by maintaining the milk within the required standards. As artificial neural networks deal with complex phenomena, their application in this field makes it possible to optimize the conditions of storage and transport of drinking milk.

Ice slurry, as a new environmentally friendly cold storage medium, is widely used in the field of cold storage and air conditioning because of its excellent flow and heat transfer characteristics. Based on experimental data of slurry flow, the rheological properties of light-particle slurries composed of polyethylene particles and water were analyzed using the response surface method. Using the yield stress and viscosity as the responses and considering three key influencing factors (solid-phase content, particle size, and pipe diameter) simultaneously, the order and law influencing the rheological factors were found. This was a new attempt to find energy-saving conditions for light slurry particle transport using the response surface method. The results showed that the response surface method can select the minimum working condition of mixed slurry viscosity and yield stress to ensure the safe and energy-saving transport of slurry. Moreover, it was also found that the main factor influencing slurry yield stress is the pipe diameter, and the yield stress increases with increasing pipe diameter. The main factor influencing slurry viscosity is particle size, and the viscosity increases with increasing particle size. [ABSTRACT FROM AUTHOR]

Abstract Vector or reservoir species of three diseases of crustaceans listed in the Animal Health Law were identified based on evidence generated through an extensive literature review, to support a possible updating of Regulation (EU) 2018/1882. Crustacean species on or in which Taura syndrome virus (TSV), Yellow head virus (YHV) or White spot syndrome virus (WSSV) were identified, in the field or during experiments, were classified as reservoir species with different levels of certainty depending on the diagnostic tests used. Where experimental evidence indicated transmission of the pathogen from a studied species to another known susceptible species, the studied species was classified as vector species. Although the quantification of the risk of spread of the pathogens by the vectors or reservoir species was not part of the terms of reference, such risks do exist for the vector species, since transmission from infected vector species to susceptible species was proven. Where evidence for transmission from infected crustaceans was not found, these were defined as reservoirs. Nonetheless, the risk of the spread of the pathogens from infected reservoir species cannot be excluded. Evidence identifying conditions that may prevent transmission by vectors during transport was collected from scientific literature. It was concluded that it is very likely to almost certain (90–100%) that WSSV, TSV and YHV will remain infective at any possible transport condition. Therefore, vector or reservoir species that may have been exposed to these pathogens in an affected area in the wild or aquaculture establishments or by water supply can possibly transmit WSSV, TSV and YHV.

Ice slurry, as a new environmentally friendly cold storage medium, is widely used in the field of cold storage and air conditioning because of its excellent flow and heat transfer characteristics. Based on experimental data of slurry flow, the rheological properties of light-particle slurries composed of polyethylene particles and water were analyzed using the response surface method. Using the yield stress and viscosity as the responses and considering three key influencing factors (solid-phase content, particle size, and pipe diameter) simultaneously, the order and law influencing the rheological factors were found. This was a new attempt to find energy-saving conditions for light slurry particle transport using the response surface method. The results showed that the response surface method can select the minimum working condition of mixed slurry viscosity and yield stress to ensure the safe and energy-saving transport of slurry. Moreover, it was also found that the main factor influencing slurry yield stress is the pipe diameter, and the yield stress increases with increasing pipe diameter. The main factor influencing slurry viscosity is particle size, and the viscosity increases with increasing particle size.

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Abstract Background A microbiological diagnosis is essential to better target antimicrobial treatment, control and prevention of respiratory tract infections in cattle. Under field conditions, non-endoscopic broncho-alveolar lavage (nBAL) samples are increasingly collected. To what extent the highly variable turnaround time and storage temperatures between sampling and cultivation affect the isolation rate of bacterial pathogens is unknown. Therefore, the objective of this experimental study was to determine the effect of different storage temperatures (0 °C, 8 °C, 23 °C and 36 °C) and times (0,2,4,6,8,24,48 h) on the isolation rate and concentration of Pasteurellaceae in nBAL samples from clinically affected animals. Results At a storage temperature temperature of 36 °C isolation rates of Mannheimia haemolytica and Pasteurella multocida were significantly reduced 6 h and 48 h after sampling, respectively. At room temperature (23 °C), a decrease in M. haemolytica and P. multocida isolation rate was noticed, starting at 24 and 48 h after sampling, respectively, but only significant for P. multocida at 48 h. The presence of microbial contamination negatively affected the isolation of P. multocida in clinical nBAL samples, but not of M. haemolytica. Conclusion Optimal M. haemolytica and P. multocida isolation rates from clinical nBAL samples are obtained after storage at 0 °C or 8 °C, provided that the sample is cultivated within 24 h after sampling. The maximum period a sample can be stored without an effect on the M. haemolytica and P. multocida isolation success varies and is dependent on the storage temperature and the degree of microbial contamination.

Environmental monitoring for Listeria monocytogenes in food processing environments is key for ensuring the safety of ready-to-eat foods. For sampling, swabs are often hydrated with a wetting or transport medium that may contain neutralizers and other ingredients. After swabbing the environment, the swabs may then be transported or shipped cold to an off-site laboratory for testing, ideally within 48 h. Extended shipping times may subject the pathogen to increased temperatures in the presence of the wetting medium, organics, and other chemicals from the processing facility that could confound detection. This study evaluated growth and detection of L. monocytogenes on stainless steel exposed to either buffer or sodium hypochlorite before drying. Swabs were rehydrated with Butterflied’s phosphate buffer, neutralizing buffer, Lethe en broth, or Dey-En gley neutralizing broth before swabbing. Swabs were stored in the presence of no added food, cheese whey, or ice cream under both optimal (48°C) and suboptimal (158°C) temperatures for up to 72 h. Overall, there was no growth of L. monocytogenes at 48°C through 72 h of storage, although enrichment from these swabs was dependent on the presence and type of food matrix. Pathogen growth during storage at 158°C was more variable and depended on both the food matrix and transport media used, with Dey-En gley and Lethe en broths allowing for the highest population increases. Overall, more enrichments resulting in L. monocytogenes detections were observed when using Lethe en broth and neutralizing buffer than Dey-En gley broth, which resulted in fewer detections at 158 C. Logistic regression and Cochran-Mantel-Haenszel analyses determined that storage temperature, transport media, and food matrix all significantly affected detection of L. monocytogenes, whereas storage time did not have a clear effect on recovery from swabs. [ABSTRACT FROM AUTHOR]

For triage purposes following a nuclear accident or a terrorist event, gene expression biomarkers in blood have been demonstrated to be good bioindicators of ionizing radiation (IR) exposure and can be used to assess the dose received by exposed individuals. Many IR-sensitive genes are regulated by the DNA damage response pathway, and modulators of this pathway could potentially affect their expression level and therefore alter accurate dose estimations. In the present study, we addressed the potential influence of temperature, sample transport conditions and the blood cell fraction analyzed on the transcriptional response of the following radiation-responsive genes: FDXR, CCNG1, MDM2, PHPT1, APOBEC3H, DDB2, SESN1, P21, PUMA, and GADD45. Whole blood from healthy donors was exposed to a 2 Gy X-ray dose with a dose rate of 0.5 Gy/min (output 13 mA, 250 kV peak, 0.2 mA) and incubated for 24 h at either 37, 22, or 4 °C. For mimicking the effect of transport conditions at different temperatures, samples incubated at 37 °C for 24 h were kept at 37, 22 or 4 °C for another 24 h. Comparisons of biomarker responses to IR between white blood cells (WBCs), peripheral blood mononuclear cells (PBMCs) and whole blood were carried out after a 2 Gy X-ray exposure and incubation at 37 °C for 24 hours. Hypothermic conditions (22 or 4 °C) following irradiation drastically inhibited transcriptional responses to IR exposure. However, sample shipment at different temperatures did not affect gene expression level except for SESN1. The transcriptional response to IR of specific genes depended on the cell fraction used, apart from FDXR, CCNG1, and SESN1. In conclusion, temperature during the incubation period and cell fraction but not the storing conditions during transport can influence the transcriptional response of specific genes. However, FDXR and CCNG1 showed a consistent response under all the different conditions tested demonstrating their reliability as individual biological dosimetry biomarkers. [ABSTRACT FROM AUTHOR]

Background: A microbiological diagnosis is essential to better target antimicrobial treatment, control and prevention of respiratory tract infections in cattle. Under field conditions, non-endoscopic broncho-alveolar lavage (nBAL) samples are increasingly collected. To what extent the highly variable turnaround time and storage temperatures between sampling and cultivation affect the isolation rate of bacterial pathogens is unknown. Therefore, the objective of this experimental study was to determine the effect of different storage temperatures (0 °C, 8 °C, 23 °C and 36 °C) and times (0,2,4,6,8,24,48 h) on the isolation rate and concentration of Pasteurellaceae in nBAL samples from clinically affected animals. Results: At a storage temperature temperature of 36 °C isolation rates of Mannheimia haemolytica and Pasteurella multocida were significantly reduced 6 h and 48 h after sampling, respectively. At room temperature (23 °C), a decrease in M. haemolytica and P. multocida isolation rate was noticed, starting at 24 and 48 h after sampling, respectively, but only significant for P. multocida at 48 h. The presence of microbial contamination negatively affected the isolation of P. multocida in clinical nBAL samples, but not of M. haemolytica. Conclusion: Optimal M. haemolytica and P. multocida isolation rates from clinical nBAL samples are obtained after storage at 0 °C or 8 °C, provided that the sample is cultivated within 24 h after sampling. The maximum period a sample can be stored without an effect on the M. haemolytica and P. multocida isolation success varies and is dependent on the storage temperature and the degree of microbial contamination. [ABSTRACT FROM AUTHOR]

The carriage of food products, in particular fresh products, is particularly sensitive to transport conditions. The aim of this paper is to examine the effect of trailer conditions on the quality of refrigerated food transport services. Six wireless measuring devices installed permanently in 3 trailers were used for the analysis. The built-in transmitter sensors have enabled the collection of data in the areas of temperature, humidity, pressure, shock and light intensity. Monitoring parameters via a wireless device can contribute to the reduction of fuel costs in the enterprise, e.g., detection with vibration sensor / acceleration of aggressive driving style of the driver causing suspended fuel consumption, detection of inappropriate transport conditions, etc. Analysis of the test results showed that in the same trailer the product distribution is very important owing to the different temperature. The analysis carried out showed that in the middle of the semitrailer, i.e. in the place where the breeze is located, temperature fluctuations amount to 5°C, which indicates a greater risk of loss of quality of the transported products. [ABSTRACT FROM AUTHOR]

Transport of beach sand to the foredune by wind is essential for dunes to grow. The aeolian sand transport rate is related to wind velocity, but wind-based models often overpredict this transport for narrow beaches (weeks).

Transporting vibration is a major cause of bruising damage in apples. The most previous studies about the bruising susceptibility of apple were conducted using the pendulum device and with a single impact. So their results for transport conditions are not accurate, because the effect of tissue fatigue (caused by cyclic loading) and apple mass have not been considered. In this study, using a vibration simulation of transportation, the effect of vibration parameters (frequency and acceleration) and fruit properties (mass, curvature radius and acoustical stiffness) on bruising susceptibility of Golden Delicious apple were investigated. The results showed that, increasing the vibration acceleration increases the bruising volume. Also increasing the vibration frequency decreased the bruising, but in higher frequencies (f ≥ 13 Hz), if the acceleration value is greater than 0.5 g, the fatigue phenomenon can reverse this trend and increasing the frequency will increase the bruising volume. Increasing the apple mass, at the acceleration of 0.5 g or more (a ≥ 0.5 g), increased the bruising volume and at accelerations of less than 0.5 g, reduced the bruising volume. Also results indicated that the apples with high acoustic stiffness and low curvature radius were more vulnerable. In conclusion, the results showed that to improve the handling of apple, arrangement of fruit in the box must be carried out carefully and we hope that findings of this research will help to achieve this goal. [ABSTRACT FROM AUTHOR]

The wind transports sand from the beach to the dunes and is therefore important for dune growth and recovery after a storm. Identifying the conditions that favour aeolian sand transport is especially important for narrow beaches, where measured long-term (seasons to years) deposition volumes on the foredune are often substantially less than the potential input from the beach. One of the most visually distinct signs of aeolian transport can be seen when relatively dry sand moves over a wet beach and organises itself to form low, slipfaceless bedforms. These features are known as sand strips. Here, we investigate the presence and characteristics of sand strips and their dependence on regional wind conditions by using a multi-year data set of video images of the Argus tower at Egmond aan Zee, The Netherlands. The dataset average wavelength and migration rate of the sand strips is 12.0 m and 1.24 m/h, respectively. Little to no relation was found between these two sand-strip characteristics and the wind velocity. The presence of these bedforms does not depend on wind velocity either, provided the wind velocity exceeds ∼ 8 m/s. Instead, the wind direction determines if fully-developed sand strips form, as they are seen during alongshore or almost alongshore winds only. Our observations are indicative of topographic steering of the wind by the 25-m high foredune into the alongshore direction, as sand strips move alongshore even under onshore-oblique, regional winds. [ABSTRACT FROM AUTHOR]

The aim of this study was to establish the impact of air transport on blood samples packaged with and without cooling elements and effect of outdoor temperature on sample quality. Venous samples from 38 blood donors in winter and 36 in summer were tested for hemolysis and complete blood count. One tube per subject was kept in controlled conditions at +4 °C. Two sets of tubes were sent by plane from Zagreb to Brussels, one with and one without cooling elements, and another two sets were sent to London following the same principle. Packages with cooling elements were stored in controlled warehousing conditions at airports (+2 °C to +8 °C), whereas packages without cooling elements were stored in ambient warehouse conditions. Data loggers were used for temperature monitoring. Our research revealed statistically significant differences in several hematologic parameters when comparing the samples stored in controlled laboratory conditions and those transported by plane. These differences were more pronounced in the samples transported during the summer. Transport conditions without cooling elements had additional negative impact on the sample quality. Transport of samples using cooling elements and controlled warehousing conditions at airports are sometimes not sufficient to maintain laboratory storage conditions., (Sestre Milosrdnice University Hospital.)

We study a linear parabolic system in a domain with a thin low-permeable inclusion. A new formulation of the problem is obtained with unknowns (u, ω). Under this approach, the main second-order parabolic equation is converted to a set of first-order partial differential equations with distributional coefficients. The relations of the approach to the classical and weak formulations of the problem are analyzed. [ABSTRACT FROM AUTHOR]

Abstract: Acclimation of organisms for ecotoxicity testing is in general processed according to Organisation for Economic Co-operation and Development (OECD) and/or Environmental Protection Agency (EPA) guidelines, under controlled conditions. However, when organisms are collected in the field, their capture, transport and adaptation to laboratory conditions are factors of stress. In their natural habitat, estuarine fish are exposed to considerable fluctuations of environmental variables, while in laboratory they are acclimated to constant conditions and this can be per se a factor of stress that may influence biomarker responses. Therefore, it is important to investigate the effects of these procedures on estuarine fish performance before using them as test organisms in ecotoxicity bioassays. The goal of the present study was to investigate the effects of transporting the common goby, Pomatoschistus microps from the field (natural populations) to the laboratory and of its acclimation to laboratory conditions on the enzymes acetylcholinesterase (AChE), lactate dehydrogenase (LDH) and glutathione S-transferases (GSTs). Fish were collected in a reference site of the Minho River estuary (NW of Portugal) and the activities of the biomarkers were monitored before and after the transport of organisms to the laboratory and during the acclimation period (at 5, 10 and 15days). The activities of all the enzymes indicated that capture and transport conditions had no effects on enzymatic activities. Furthermore, AChE, LDH and GST presented higher activities at the end of the acclimation period than at beginning, suggesting a physiological adaptation to laboratory conditions. This adaptation should be taken into consideration in the experimental design to avoid bias in the interpretation of effects of xenobiotics on biomarkers. [Copyright &y& Elsevier]

Transport of beach sand to the foredune by wind is essential for dunes to grow. The aeolian sand transport rate is related to wind velocity, but wind-based models often overpredict this transport for narrow beaches (<100 m). To better predict aeolian sand transport, the fetch-based Aeolus model was developed. Here, we qualitatively test this model by comparing its transport-rate output to visual signs of aeolian transport on video imagery collected at Egmond aan Zee, the Netherlands, during a six-month winter period. The Aeolus model and the Argus images often agree on the timing of aeolian transport days, except when transport is small; that is not always visible on the Argus images. Consistent with the imagery (minimal signs of aeolian activity in strong winds), the Aeolus model sometimes predicts the actual transport to be smaller than the potential transport. This difference is largest when wind velocity is large, and its direction is cross-shore. Although transport limitations are not predicted to be common, the results suggest that their effect on the total transport in the study period was substantial. This indicates that the fetch distance should be taken into account when calculating aeolian transport for narrow beaches on longer timescales (>weeks). [ABSTRACT FROM AUTHOR]

The global coronavirus (CoV) disease 2019 (COVID-19) pandemic has resulted in a worldwide shortage of viral transport media and raised questions about specimen stability. The objective of this study was to determine the stability of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) RNA in specimen transport media under various storage conditions. Transport media tested included UTM, UTM-RT, ESwab, M4, and saline (0.9% NaCl). Specimen types tested included nasopharyngeal/oropharyngeal swabs in the above-named transport media, bronchoalveolar lavage (BAL) fluid, and sputum. A high-titer SARS-CoV-2 remnant patient specimen was spiked into pooled SARS-CoV-2 RNA-negative specimen remnants for the various medium types. Aliquots of samples were stored at 18°C to 26°C, 2°C to 8°C, and -10°C to -30°C and then tested at time points up to 14 days. Specimens consistently yielded amplifiable RNA with mean cycle threshold differences of <3 over the various conditions assayed, thus supporting the use and transport of alternative collection media and specimen types under a variety of temperature storage conditions., (Copyright © 2020 Rogers et al.)