1. Expression of ERG Protein and TMRPSS2-ERG Fusion in Prostatic Carcinoma in Egyptian Patients
- Author
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Mohamed Badawy, Hani H. Nour, Shady E. Anis, Nora N. Kamel, Amr El Kholy, Olfat Hammam, Sara Diab, Afaf Ahmed Abdel-Hady, Soulafa Abd El-Aziz, Ali El-Hindawi, Noha A.H. Helmy, Maha Akl, Ahmed Abdel-Hady, Heba Khalil, Ahmed Ismail, Khalid Al Osili, and This work was financed by TBRI internal project No. 107T. Principle investigator: Prof. Dr Ahmed Abdel Hadi.
- Subjects
TMRPSS2-ERG ,Pathology ,medicine.medical_specialty ,genetic structures ,Urology ,030232 urology & nephrology ,lcsh:Medicine ,Prostatic carcinoma ,03 medical and health sciences ,Prostate cancer ,ERG Immunoexpression ,Fluorescence in situ hybridization (FISH) ,High grade prostatic intraepithelial neoplasia ,0302 clinical medicine ,Immunophenotyping ,Basic Science ,Prostate ,Carcinoma ,Medicine ,High-grade prostatic intraepithelial neoplasia ,business.industry ,lcsh:R ,General Medicine ,medicine.disease ,eye diseases ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Monoclonal ,Immunohistochemistry ,sense organs ,business ,Erg - Abstract
AIM: Prostate cancer (PCa) is the second most common cancers in men worldwide. Its incidence can be influenced by several risk factors including genetic susceptibility. Therefore the search for the expression of a certain gene (ERG) and its rearrangement could give us clues for proper identification of PCa. And the study of ERG expression and its comparison to FISH in Egyptian patients can show whether ERG immunophenotype could be used instead of FISH, as it is cheaper.MATERIALS AND METHODS: This study was performed on 85 cases of PCa, showing 30 cases with HGPIN and 30 cases of prostatic hyperplasia. All were immunohistochemistry stained using ERG monoclonal rabbit antihuman antibody was used (clone: EP111). FISH analysis was performed in 38 biopsies of PCa cases to detect TMRPSS2-ERG rearrangement using the FISH ZytoLight TriCheck Probe (SPEC TMRPSS2-ERG).RESULTS: ERG expression was found in 26% of PCa cases and 20% of HGPIN cases. FISH analysis showed fusion of 21 cases of PCa (out of 22 cases showing ERG immunoexpression).CONCLUSION: Our findings emphasise that only malignant and pre-malignant cells and not benign cells from the prostate stain positive. ERG expression may offer a simpler, accurate and less costly alternative for evaluation of ERG fusion status in PCa.
- Published
- 2017