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Expression of ERG Protein and TMRPSS2-ERG Fusion in Prostatic Carcinoma in Egyptian Patients
- Source :
- Open Access Macedonian Journal of Medical Sciences, Open Access Macedonian Journal of Medical Sciences; Vol. 5 No. 2 (2017): Apr 15 (OAMJMS); 147-154, Open Access Macedonian Journal of Medical Sciences; Vol 5, No 2 (2017): Apr 15 (OAMJMS); 147-154, Open Access Macedonian Journal of Medical Sciences, Vol 5, Iss 2, Pp 147-154 (2017)
- Publication Year :
- 2017
-
Abstract
- AIM: Prostate cancer (PCa) is the second most common cancers in men worldwide. Its incidence can be influenced by several risk factors including genetic susceptibility. Therefore the search for the expression of a certain gene (ERG) and its rearrangement could give us clues for proper identification of PCa. And the study of ERG expression and its comparison to FISH in Egyptian patients can show whether ERG immunophenotype could be used instead of FISH, as it is cheaper.MATERIALS AND METHODS: This study was performed on 85 cases of PCa, showing 30 cases with HGPIN and 30 cases of prostatic hyperplasia. All were immunohistochemistry stained using ERG monoclonal rabbit antihuman antibody was used (clone: EP111). FISH analysis was performed in 38 biopsies of PCa cases to detect TMRPSS2-ERG rearrangement using the FISH ZytoLight TriCheck Probe (SPEC TMRPSS2-ERG).RESULTS: ERG expression was found in 26% of PCa cases and 20% of HGPIN cases. FISH analysis showed fusion of 21 cases of PCa (out of 22 cases showing ERG immunoexpression).CONCLUSION: Our findings emphasise that only malignant and pre-malignant cells and not benign cells from the prostate stain positive. ERG expression may offer a simpler, accurate and less costly alternative for evaluation of ERG fusion status in PCa.
- Subjects :
- TMRPSS2-ERG
Pathology
medicine.medical_specialty
genetic structures
Urology
030232 urology & nephrology
lcsh:Medicine
Prostatic carcinoma
03 medical and health sciences
Prostate cancer
ERG Immunoexpression
Fluorescence in situ hybridization (FISH)
High grade prostatic intraepithelial neoplasia
0302 clinical medicine
Immunophenotyping
Basic Science
Prostate
Carcinoma
Medicine
High-grade prostatic intraepithelial neoplasia
business.industry
lcsh:R
General Medicine
medicine.disease
eye diseases
medicine.anatomical_structure
030220 oncology & carcinogenesis
Monoclonal
Immunohistochemistry
sense organs
business
Erg
Subjects
Details
- ISSN :
- 18579655
- Volume :
- 5
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Open access Macedonian journal of medical sciences
- Accession number :
- edsair.doi.dedup.....e8e94a00405a8f5093f7bcd0092bf277