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Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Yellowfin tuna (Thunnus albacares) is a valuable pelagic migratory fish with potential for aquaculture. Despite this, there is limited understanding of the biological and physiological characteristics of this species, particularly regarding sex differences in growth performance. The liver, a crucial organ for digestion and metabolism, plays a significant role in regulating fish growth. This study aimed to compare liver enzyme activities and transcriptome profiles between female and male yellowfin tuna to uncover the molecular mechanisms underlying difference between the sexes. The results revealed that female yellowfin tuna exhibited higher amylase and lipid metabolism enzyme activities, while male yellowfin tuna showed higher glucose-6-phosphate dehydrogenase and antioxidant enzyme activities. Additionally, through Illumina sequencing technology, the study generated 37.74 Gb of clean data and identified 36,482 unique genes (UniGenes) in the liver transcriptome. A total of 2542 differentially expressed genes were found, with enriched Gene Ontology terms and pathways related to metabolic processes, particularly lipid metabolism and transport. These findings suggest that female yellowfin tuna have superior digestive enzyme activities and lipid metabolism, while male yellowfin tuna excel in sugar metabolism, ATP production, and antioxidant defense. This study provides valuable insights into sex differences in yellowfin tuna and could aid in advancing full-cycle aquaculture practices for this species. [ABSTRACT FROM AUTHOR]

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The tuna fishery resources, particularly yellowfin tuna (YFT), in Ternate waters, North Maluku, Indonesia (fishery management area 715), are currently classified as fully exploited at 0.97% utilization rate of the available potentials, i.e., 31.659 ton/year. This calls for an attempt to manage the yellowfin tuna in such a way that its existence in the waters can be maintained. Studies on fish population parameters are an effort to obtain scientific information as a recommendation for sustainable fisheries management. This research used experimental fishing by measuring the fish's length. Then, its population was estimated using FISAT II program, and its spawning potential ratio (SPR) was analyzed using length based-spawning potential ratio (LB-SPR) software. It was expected that this would be able to give the yellowfin tuna current status and condition in Ternate waters. Conducted from May through August 2023, and analyzing 4,904 fish, the research managed to determine the population parameters of yellowfin tuna in Ternate waters. Its asymptotic length (L8), growth coefficient (K) and t0 were 117.60 cm, 1.4 per year, and 0.17 year, respectively. These were then plotted to obtain a von Bertalanffy growth curve using the formula Lt = 117,60*(1-Exp-1,4(t-0,17). From the analysis, it was found that the fish total mortality, natural mortality and captive mortality rates were 3.89, 1.55, and 2.34, respectively. Furthermore, it was found that recruitment occurred twice a year, i.e., on March and August at 6.72 and 25.18%, with the Y/R value being 0.039 and B/R value of 0.482. Meanwhile, the spawning potential ratio (SPR) value was 19%, indicating that the utilization rate had been over exploited (SPR< 20%) and it was supported by the fact that the exploitation rate value had been over 0.60 (over fishing) from the Emax value (0.421). Therefore, management is needed to ensure the sustainability of yellowfin tuna stocks. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the changes in the formation of biogenic amines and free fatty acids occurring during the manufacturing process of a traditional dry-cured product based on yellowfin tuna, Thunnus albacares (protected geographical indication—Mojama) and how the different processing stages could affect food safety aspects. The biogenic amines profile was determined by HPLC–DAD, following the official methodology, and free fatty acids were quantified by GC–MS. Histamine levels found in all stages of the manufacturing process did not exceed the maximum limits established in the European Commission (100–200 mg/kg) and US Food and Drug Administration (50 mg/kg) regulations. Other biogenic amines, such as cadaverine and putrescine, were detected at low level or below the limit of detection. Yellowfin tuna filets could be classified as lean fish flesh, presenting 1.18% fat on average. An increment in the free fatty acid fraction was evidenced along the manufacturing process, ranging from 10.37% of the total lipids in fresh loins to 16.88% in the dry-cured filet product. The results indicated that the traditional manufacturing process of mojama, consisting of salting and drying tuna loins kept at a controlled temperature, promoted a moderate lipolysis phenomenon, and the formation of free fatty acids with high proportions of unsaturated fatty acids, likely arising from the lipolysis of muscle phospholipids. [ABSTRACT FROM AUTHOR]

Tuna is an excellent food product, relatively low in calories, that is recommended for a balanced diet. The continuously increasing demand, especially for bluefin-tuna-based food preparations, and its relatively high market price make adulteration by intentionally mixing with other lower-priced tunas more prospective. The development of rapid methods to detect tuna adulteration is a great challenge in food analytical science. We have thus developed a simple, fast, and low-cost molecular rapid test for the visual detection of tuna adulteration. It is the first sensor developed for tuna authenticity testing. The three species studied were Thunnus thynnus (BFT), Thunnus albacares, and Katsuwonus pelamis. DNA was isolated from fresh and heat-treated cooked fish samples followed by PCR. The PCR products were hybridized (10 min) to specific probes and applied to the rapid sensing device. The signal was observed visually in 10–15 min using gold nanoparticle reporters. The method was evaluated employing binary mixtures of PCR products from fresh tissues and mixtures of DNA isolates from heat-treated tissues (canned products) at adulteration percentages of 1–100%. The results showed that the method was reproducible and specific for each tuna species. As low as 1% of tuna adulteration was detected with the naked eye. [ABSTRACT FROM AUTHOR]

Background: Considering that the tuna fishes are used as raw material for preparing canned fish, therefore, it is necessary to identify the most suitable method of catching and keeping them in order to maintain the nutritional value and quality of the raw material. Aims: In this study, the proximate composition and quality of Thunnus tonggol and Thunnus albacares unloaded in Chabahar beach were investigated based on the type of fishing and storage method in fishing vessels. Methods: After landing the fish, they were immediately transferred to the laboratory of Chabahar maritime university and then the proximate composition (moisture, protein, fat and ash) and chemical quality indicators (pH, peroxide Value, TBA acid, anisidine index, TMA and TVB-N) were measured. Results: In Thunnus tonggol caught with gillnet and kept in ice the amount of moisture, protein and fat was significantly lower compared to other groups (P<0.05). In Thunnus albacares there were no significant differences in proximate compounds. Among the chemical indicators, pH level in Thunnus tonggol fish caught with gillnet and kept in ice increased significantly (P<0.05) and in Thunnus albacares fish caught with hooks and kept in freezing condition, decreased significantly (P<0.05). The peroxide value, TBA and TMA in fishes caught with hook and kept in frozen condition was significantly lower than other groups, but anisidine index did not show significant difference in all samples. Conclusion: Overall, the results showed that fishes caught with hooks and kept in frozen condition had better nutritional value and quality than other groups. [ABSTRACT FROM AUTHOR]

Background: The Yellowfin tuna (Thunnus albacares) is a large tuna exploited by major fisheries in tropical and subtropical waters of all oceans except the Mediterranean Sea. Genomic studies of population structure, adaptive variation or of the genetic basis of phenotypic traits are needed to inform fisheries management but are currently limited by the lack of a reference genome for this species. Here we report a draft genome assembly and a linkage map for use in genomic studies of T. albacares. Methods and results: Illumina and PacBio SMRT sequencing were used in combination to generate a hybrid assembly that comprises 743,073,847 base pairs contained in 2,661 scaffolds. The assembly has a N50 of 351,587 and complete and partial BUSCO scores of 86.47% and 3.63%, respectively. Double-digest restriction associated DNA (ddRAD) was used to genotype the 2 parents and 164 of their F1 offspring resulting from a controlled breeding cross, retaining 19,469 biallelic single nucleotide polymorphism (SNP) loci. The SNP loci were used to construct a linkage map that features 24 linkage groups that represent the 24 chromosomes of yellowfin tuna. The male and female maps span 1,243.8 cM and 1,222.9 cM, respectively. The map was used to anchor the assembly in 24 super-scaffolds that contain 79% of the yellowfin tuna genome. Gene prediction identified 46,992 putative genes 20,203 of which could be annotated via gene ontology. Conclusions: The draft reference will be valuable to interpret studies of genome wide variation in T. albacares and other Scombroid species. [ABSTRACT FROM AUTHOR]

Introduction: Tuna has a cost-effective energy supply to support the regional endothermic and high-speed swimming performance. The gut symbiotic microbiotas and their metabolites play essential roles in tuna's diet digestion, absorption, and energy acquirement, which are often highly related to the ontogenetic development of tuna. Methods: We compared gut microbial compositions and metabolites, as well as mRNA expression of the intestine between juvenile and adult yellowfin tuna using 16S rRNA sequencing, metabolomic and transcriptomic, respectively. Results and discussion: The results revealed that adults had a significantly higher microbial diversity and abundance of Acinetobacter than juveniles. Regarding the gut microbiota-derived metabolites, fatty acids, especially glycerophospholipid and sphingolipid, were significantly enriched in adults than in juveniles. Moreover, the short-chain fatty acid (butyrate and isobutyrate) contents were significantly higher in adults than in juveniles. To find the relationship between gut microbiotas and host physiology, intestinal transcriptome analysis demonstrated that the enriched pathways of differential expression genes (DEGs) in adult tuna were the lipid metabolism pathway, including "fat digestion and absorption," "cholesterol metabolism," "steroid hormone biosynthesis," "glycerolipid metabolism," and "glycerophospholipid metabolism." However, protein digestion and absorption and pancreatic secretion pathways were significantly enriched in the juveniles. The conjoint analysis indicated that the enriched pathways of both differential metabolites (DMs) and DEGs were remarkably related to the regulation of glycerophospholipids metabolism in adult tunas. This study highlights the role of gut microbiotas in fish nutrition metabolism. These findings provide new insights into the view of ontogenetic shifts of gut microbiotas and their metabolites on host health and gut function in endothermic and high-speed swimming marine fish species. [ABSTRACT FROM AUTHOR]

Recent studies argue for the presence of genetic population structure in yellowfin tuna (Thunnus albacares) in all oceans. However, the persistence of family groups has never been considered a viable mechanism of structure, nor has it been measured. We analyzed genetic similarity among 280 yellowfin tunas from seven population samples collected in the Western and Central Pacific Ocean (WCPO) using single nucleotide polymorphisms, and found population structure that was significantly explained by the presence of 96 individuals involved in 332 half or full sib dyads. We found significantly higher mean and median relatedness between individuals from the same sample groups, compared to individuals from different groups; and high relatedness between individuals caught at the same fish-aggregating device (FAD) than between those caught in the wider exclusive economic zone (EEZ). Alternatively, the EEZ of the Federated States of Micronesia may harbor exceptionally large numbers of close kin. We conclude that yellowfin directly school with related individuals through their first year, and at least demonstrate tightly overlapping regional fidelity as adults. These results may explain, to some extent, the patterns of population genetic structure recently observed in yellowfin tuna. [ABSTRACT FROM AUTHOR]

Copyright of Indonesian Fisheries Processing Journal / Jurnal Pengolahan Hasil Perikanan Indonesia is the property of IPB University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Yellowfin tuna (Thunnus albacares) is a valuable pelagic migratory fish with potential for aquaculture. Despite this, there is limited understanding of the biological and physiological characteristics of this species, particularly regarding sex differences in growth performance. The liver, a crucial organ for digestion and metabolism, plays a significant role in regulating fish growth. This study aimed to compare liver enzyme activities and transcriptome profiles between female and male yellowfin tuna to uncover the molecular mechanisms underlying difference between the sexes. The results revealed that female yellowfin tuna exhibited higher amylase and lipid metabolism enzyme activities, while male yellowfin tuna showed higher glucose-6-phosphate dehydrogenase and antioxidant enzyme activities. Additionally, through Illumina sequencing technology, the study generated 37.74 Gb of clean data and identified 36,482 unique genes (UniGenes) in the liver transcriptome. A total of 2542 differentially expressed genes were found, with enriched Gene Ontology terms and pathways related to metabolic processes, particularly lipid metabolism and transport. These findings suggest that female yellowfin tuna have superior digestive enzyme activities and lipid metabolism, while male yellowfin tuna excel in sugar metabolism, ATP production, and antioxidant defense. This study provides valuable insights into sex differences in yellowfin tuna and could aid in advancing full-cycle aquaculture practices for this species.

Carbon stable isotopes (δ13C) are widely used in ecological studies to understand diet, food web dynamics, and movements of marine fishes. Still, δ13C is influenced by lipid content and often requires chemical extraction or mathematical correction. Here, we developed a species-specific mathematical lipid correction for white muscle tissue of yellowfin tuna (Thunnus albacares), a highly migratory finfish of considerable economic and ecological value. Lipid extraction was conducted on yellowfin tuna white muscle tissue (C:N range: 2.96–6.49), and both linear and non-linear lipid correction models for δ13C were fitted and assessed. Lipid extraction increased δ13C, and to a lesser extent, δ15N values in yellowfin tuna white muscle tissue, but had no effect on δ34S. Both non-linear models provided better fits to the data than the linear model, suggesting an asymptotic relationship between C:N and ∆δ13C. Results support the growing body of evidence that C:N ratios can be used to predict lipid corrected δ13C and highlight the value of mathematical correction approaches. We provide species-specific parameter estimates that can be used for lipid correction of white muscle tissue for δ13C analysis in yellowfin tuna and similar species for which species-specific models have yet to be developed. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Tuna is an excellent food product, relatively low in calories, that is recommended for a balanced diet. The continuously increasing demand, especially for bluefin-tuna-based food preparations, and its relatively high market price make adulteration by intentionally mixing with other lower-priced tunas more prospective. The development of rapid methods to detect tuna adulteration is a great challenge in food analytical science. We have thus developed a simple, fast, and low-cost molecular rapid test for the visual detection of tuna adulteration. It is the first sensor developed for tuna authenticity testing. The three species studied were Thunnus thynnus (BFT), Thunnus albacares, and Katsuwonus pelamis. DNA was isolated from fresh and heat-treated cooked fish samples followed by PCR. The PCR products were hybridized (10 min) to specific probes and applied to the rapid sensing device. The signal was observed visually in 10–15 min using gold nanoparticle reporters. The method was evaluated employing binary mixtures of PCR products from fresh tissues and mixtures of DNA isolates from heat-treated tissues (canned products) at adulteration percentages of 1–100%. The results showed that the method was reproducible and specific for each tuna species. As low as 1% of tuna adulteration was detected with the naked eye.

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study analyses the mercury (Hg) concentration in the meat of Thunnus albacares and Thunnus obesus caught from the western equatorial Atlantic Ocean. The objective was to estimate the Hg intake via tuna ingestion and presents the possible health risk assessment. For T. albacares and T. obesus, the median concentration was 212 ng·g-1 and 475.1 ng·g-1 wet weight, respectively. The Hg concentrations were below the maximum tolerable limit established by international and Brazilian regulations for fishery products. The consumption would pose a risk for human populations that ingest more than 80 g·day-1. Regular monitoring of both human consumption rates and Hg levels in fish are recommended. [ABSTRACT FROM AUTHOR]

Carbon stable isotopes (δ13C) are widely used in ecological studies to understand diet, food web dynamics, and movements of marine fishes. Still, δ13C is influenced by lipid content and often requires chemical extraction or mathematical correction. Here, we developed a species-specific mathematical lipid correction for white muscle tissue of yellowfin tuna (Thunnus albacares), a highly migratory finfish of considerable economic and ecological value. Lipid extraction was conducted on yellowfin tuna white muscle tissue (C:N range: 2.96–6.49), and both linear and non-linear lipid correction models for δ13C were fitted and assessed. Lipid extraction increased δ13C, and to a lesser extent, δ15N values in yellowfin tuna white muscle tissue, but had no effect on δ34S. Both non-linear models provided better fits to the data than the linear model, suggesting an asymptotic relationship between C:N and ∆δ13C. Results support the growing body of evidence that C:N ratios can be used to predict lipid corrected δ13C and highlight the value of mathematical correction approaches. We provide species-specific parameter estimates that can be used for lipid correction of white muscle tissue for δ13C analysis in yellowfin tuna and similar species for which species-specific models have yet to be developed.

A total of 756 yellowfin tuna (Thunnus albacares) caught by a Chinese drifting longliner in the tropical western and central Pacific Ocean (WCPO) from May 2018 to March 2019 were investigated to describe the reproductive biology of the species. Generalized linear model and polytomous logistic regression for the ordinal response model were employed to assess the effects of biometric and spatiotemporal factors (such as individual fork length (FL), fishing depth, dissolved oxygen, and month) on the reproductive traits of yellowfin tuna. The results showed that FLs ranged from 87 to 163 cm, averaging 115.8 cm (SD = ±14.2) for females and 121.8 cm (SD = ±16.8) for males. The proportion of males in the sampled fish was 0.61 (SD = ±0.29), and larger males (>130 cm) were proportionally predominant. Analyses based on the monthly variation of the gonadosomatic index and monthly proportion of sexual maturity stages of the gonads showed that the main spawning period of yellowfin tuna lasts from September to December. In addition, the 50% first maturity FLs of males and females were 111.96 cm (SD = ±1.04) and 119.64 cm (SD = ±1.30), respectively. This study provides new information on the reproductive development of T. albacares in the tropical WCPO region. These reproductive parameters reduce uncertainty in current stock assessment models, which will ultimately assist the fishery in becoming sustainable for future generations. [ABSTRACT FROM AUTHOR]

In the study, the parasite from the yellowfin tuna (Thunnus albacares) was separated, and morphological observation and molecular identification were carried out. Our results showed that the parasite was similar to Pennella sp. Its cephalothorax was covered by spherical to spherical non-branched nipples of almost the same size, which were very similar in shape and arrangement. A pair of slightly larger, the unbranched antenna was present on the outer margin of the small papillae-covered area. The gene sequence of COX1 with a length of 1,558 bp in the mitochondria of the parasite was 100% similar to Pennella sp. (MZ934363). The mitochondrial genome had a total length of 14,620 bp. It consisted of 36 genes (12 protein-coding, 22 transfer RNAs and 2 ribosomal RNAs) and a dummy control region, but the mitochondrial genome had no ATP8 gene. Morphological observation showed that Pennella sp. was dark red, with a convex cephalothorax, with a total length of 8.42 cm, parasitic on the dorsal side of yellowfin tuna. Pennella sp. included the cephalothorax, neck, trunk, abdomen and egg belt. This study was the first report on the mitochondrial genome of Pennella sp. The results provide basic data for further identifying the parasites of Pennella genus. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Recent anecdotal reports from seafood processors in eastern Australia have described an increased occurrence of post-mortem myoliquefaction ('jellymeat') in broadbill swordfish Xiphias gladius, and macroscopic cysts throughout the musculature of yellowfin tuna Thunnus albacares. A genus of parasitic cnidarians, Kudoa (Myxosporea, Multivalvulida), species of which are known to occur in economically important wild-caught fish species globally, can cause similar quality-deterioration issues. However, Kudoa sp. epizootiology within commercially harvested, high-value fish caught within Australia is poorly understood, despite the parasite's economic importance. To determine the causative agent responsible for the observed quality deterioration in swordfish and yellowfin tuna, muscle-tissue samples from seafood processors in Mooloolaba, Australia, collected from October 2019–February 2020, were examined for parasitic infection. Kudoid myxospores were identified from both hosts and were subquadrate in shape, with four equal-sized polar capsules. The SSU rDNA sequences from both fish shared > 99% identity to Kudoa species. Kudoa musculoliquefaciens was isolated from 87.1% of swordfish sampled, suggesting that it is a widespread parasite in swordfish from the southwest Pacific Ocean. This study provides the first molecular and morphological characterisation of Kudoa thunni in yellowfin tuna and K. musculoliquefaciens in swordfish harvested from the waters of eastern Australia, expanding the geographical distribution of K. thunni and K. musculoliquefaciens to include the Coral and Tasman Seas. We demonstrate that not all infected swordfish progress to jellymeat, show the usefulness of molecular tools for reliably identifying infection by Kudoa spp., and add to the overall knowledge of kudoid epizootiology in wild-caught fish. [ABSTRACT FROM AUTHOR]

Assessing fishing capacity and biological reference points are basic parameters for the conservation of fishery resources. The catch-based Monte Carlo maximum sustainable yield (CMSY) and Bayesian state-space Schaefer production model (BSM) were applied to assess the fishing capacity of Yellowfin tuna (YFT) (Thunnus albacares) and Longtail tuna (LTT) (Thunnus tonggol) fishery from Pakistan. Long-term and short-term data series was used to evaluate the robust information to avoid the change in data series error. Twenty-two years and fifteen years catch and catch per unit efforts (CPUE) data were used to evaluate the stock status. The estimated values of YFT from twenty-four years of BSM and CMSY model at r = 0.034, k = 1015, and MSY = 8.86, r = 0.47, k = 718 and MSY = 8.82 respectively. Biomass and exploitation rate were at B/B MSY = 0.515 and F/F MSY = 1.83 respectively. The biological reference points from BSM and CMSY model of YFT using 2007–2021 data were r = 0.034, k = 966 and MSY = 8.57, r = 0.077, k = 340 and MSY = 5.77 respectively. B 2021 /B MSY and F/F MSY were at 0.54 and 1.83 respectively indicating higher values than the fishing capacity. While, the CMSY estimated values for LTT using long- and short-term data were r = 0.04, 0.05; k = 603, 567; MSY = 7.45; 7.33 respectively. Whereas, BSM values r = 0.33; 0.31, k = 922; 923, MSY = 7.73; 7.34 respectively. The estimated BSM B 2021 /B MSY (<1.0) and F 2021 /F MSY (>1.0) for both species were higher than the fishing capacity limits. The results from both models using two different time series data indicates that the stock of this important fishery is in overexploited state. Present findings provide the fishing capacity to set the harvest control point for tuna fishery. It is advised to monitor illegal, unreported and unregulated fishing activities. The fishery managers should adopt management steps to maintain the stock of tuna fishery from Pakistan, northern Arabian Sea. • First time study on stock assessment of important fish species of Tuna fishery were conducted from northern Arabian Sea Pakistan. • Now a days CMSY and BSM models are frequently used to assess the fish stock assessment for better fishery management. • The estimated BSM B 2021 /B MSY (<1.0) and F 2021 /F MSY (>1.0) for both species were higher than the fishing capacity limits. • The results from models using different time series data indicates that the stock of fishery is overexploited. • Present findings provide the fishing capacity to set the harvest control point for tuna fishery. [ABSTRACT FROM AUTHOR]

A total of 756 yellowfin tuna (Thunnus albacares) caught by a Chinese drifting longliner in the tropical western and central Pacific Ocean (WCPO) from May 2018 to March 2019 were investigated to describe the reproductive biology of the species. Generalized linear model and polytomous logistic regression for the ordinal response model were employed to assess the effects of biometric and spatiotemporal factors (such as individual fork length (FL), fishing depth, dissolved oxygen, and month) on the reproductive traits of yellowfin tuna. The results showed that FLs ranged from 87 to 163 cm, averaging 115.8 cm (SD = ±14.2) for females and 121.8 cm (SD = ±16.8) for males. The proportion of males in the sampled fish was 0.61 (SD = ±0.29), and larger males (>130 cm) were proportionally predominant. Analyses based on the monthly variation of the gonadosomatic index and monthly proportion of sexual maturity stages of the gonads showed that the main spawning period of yellowfin tuna lasts from September to December. In addition, the 50% first maturity FLs of males and females were 111.96 cm (SD = ±1.04) and 119.64 cm (SD = ±1.30), respectively. This study provides new information on the reproductive development of T. albacares in the tropical WCPO region. These reproductive parameters reduce uncertainty in current stock assessment models, which will ultimately assist the fishery in becoming sustainable for future generations.

Yellowfin tuna are the mainstay of the traditional tuna fisheries in St Helena waters, but there is limited knowledge of their ecology and feeding behaviour in the area. In this study yellowfin tuna stomach contents were used to assess spatio‐temporal changes in feeding strategy and consider the role of tuna in the local ecosystem. Comparisons of the feeding spectra of yellowfin tuna between inshore regions of St Helena and oceanic seamounts demonstrated that in both areas the species was largely piscivorous. In inshore waters yellowfin consumed more neritic fauna, including significant numbers of crab megalopa, whereas around seamounts the diet included a greater diversity of epi‐ and mesopelagic fish and squids. The most important fish prey species in inshore waters was the St Helena butterflyfish Chaetodon sanctahelenae, and around seamounts was the pufferfish Lagocephalus lagocephalus. Results indicate that the diet spectrum of yellowfin tuna in St Helena waters is relatively similar to those of conspecifics living in waters with relatively low productivity, with strategies indicative of food‐poor ecosystems. The availability of coastal fauna may make areas around islands and seamounts more attractive for feeding aggregations of yellowfin tuna, compared to the open ocean. The relatively unselective feeding of yellowfin tuna means that stomachs can provide valuable data on the species diversity, particularly in remote areas with limited opportunities for dedicated research expeditions. [ABSTRACT FROM AUTHOR]

The efficacy of large marine protected areas (MPA) for the conservation of mobile pelagic species is widely debated. Here, we quantified spatial and temporal trends in standardized catch per unit effort (CPUE) of two target pelagic species, yellowfin (Thunnus albacares) and bigeye tuna (Thunnus obesus) in the Indian Ocean to analyze the impact of the British Indian Ocean Territory (BIOT) MPA’s creation in 2010. We applied generalized additive mixed effects models to historical BIOT longline fishery logbooks and official catch and effort statistics from the Indian Ocean Tuna Commission (IOTC) to standardize CPUEs and to evaluate long-term trends and distribution patterns across the equatorial Indian Ocean. We find that trends in population indices from within BIOT prior to the MPA’s establishment mirror those of the wider Indian Ocean. We also suggest that there may be behavioral plasticity within the yellowfin tuna stock, with some individuals possibly undertaking alternative migration patterns or exhibiting residency behavior within BIOT. Yet, we find no direct evidence of any improvement in standardized CPUE indices of either species in the area surrounding the BIOT MPA, nearly 8 years after its establishment. The average size of yellowfin and bigeye tunas caught both increased after the MPA’s establishment. These patterns were also evident across the equatorial Indian Ocean, suggesting that any MPA effect is in combination with other regional drivers, such as behavioral changes in the fishing fleets in response to the threat of Somali Piracy. We therefore conclude that BIOT MPA may be providing local conservation value to tunas, but it is not significant enough to halt the overall regional decline in yellowfin tuna stocks. Thus, we emphasize that large MPAs should be considered in conjunction with other fishery management regulations and wider regional processes, rather than as a silver bullet, in order to provide the conservation and management benefits needed for mobile species like tunas.

The aim of this study was to evaluate the influence of the fishing location on yellowfin tuna’s (YFT; Thunnus albacares) white muscle total lipid (TL) content and fatty acid profile. The comparison included 45 YFT loins, equally divided between the Atlantic, Indian and Pacific Oceans. The ocean had no significant influence on YFT TL content, total PUFA and total n-3 PUFA (p > 0.05), averaging 1.09 g/100 g of muscle, 229.2 and 192.8 mg/100 g of muscle, respectively. On the other hand, the YFT harvested on the Indian Ocean displayed significantly higher contents of both SFA and MUFA totals than the Atlantic Ocean counterparts (p < 0.05), while the YFT harvested in the Pacific Ocean presented intermediate values, not differing significantly from the other two origins. The YFT from the Indian and Pacific oceans held twice the n-6 PUFA content recorded in the Atlantic YFT (44.1 versus 21.1 mg/100 g of muscle). Considering the recommended daily intake of EPA plus DHA is 250 mg, the consumption of 100 g of YFT from the Atlantic, Indian and Pacific Oceans would provide 149.2 mg, 191.8 mg or 229.6 mg of EPA plus DHA, representing 59.7%, 76.7% or 91.8% of the recommended daily intake, respectively.

Tuna fisheries are the most valuable fisheries in the world, with an estimated market value of at least US$42 billion in 2018. Indonesia plays an important role in the global tuna fisheries and has committed to improve its fisheries management; therefore, a pilot of long-term spatial-temporal data bases was developed in 2012, however none have utilized data to have better understanding for management improvement. In this study, the annual and seasonal variation of large (10 kg) Yellowfin Tuna (YFT) catch per unit effort (CPUE) have been investigated and the influence of sea surface temperature (SST) and chlorophyll-a on these variables examined. We used fish landing data fromWest Nusa Tenggara recorded every month between 2012 and 2017 and analyzed using generalized linear models and generalized additive models. We found a seasonal and annual pattern of tuna abundance affected by SST and chlorophyll-a (chl a) and related to upwelling and El Nino event. These results also suggest that a two-month closure to fishing in August and September in southern Lombok is worth considering by the Government to maximize conservation of stocks due to a high abundance of juveniles emerging during the upwelling months from June to August. [ABSTRACT FROM AUTHOR]

Gelatin, which is thermally converted from collagen, is widely utilized in the pharmaceutical industry as a raw material for hard and soft capsules. The aim of this study was to produce gelatin from yellowfin tuna skin, the by-product of tuna processing, and determine the optimal concentration for hard capsule production. Capsules were prepared from the skin gelatin. The tuna skin gelatin was characterized as follows: pH 6.0, gel strength 191 g Bloom, water content 7.01%, ash content 0.92, setting temperature 22.3 °C, and viscosity 12,700 cP. A 20% gelatin solution was found to be the best concentration among the concentrations examined for hard capsule production, and gave a body capsule height of 19.4 mm, cap capsule height of 12.8 mm, weight of 0.07 g, disintegration time of 4.09 min, pH of 5.92, and moisture content of 12.0%. The results suggest that tuna skin gelatin can be an excellent source of hard capsule material. [ABSTRACT FROM AUTHOR]

The fishery and stock structure of Thunnus albacares off Andhra waters along western Bay of Bengal was studied from 2012 to 2015. The average annual landing of the species during the study period was 5,952 t contributing 32.9 % of the total tuna landings. Gill nets contributed 59 % of the catches, followed by hook and lines (34 %). Von Bertalanffy growth equation was Lt = 196.35 [1 – e –0.23 (t+0.0546)] and natural mortality, fishing mortality and total mortality was 0.4, 0.71 and 1.11 with exploitation ratio of 0.64 and exploitation rate of 0.43. Annual average stock, standing stock biomass and maximum sustainable yield were 13,879 t, 8,383 t and 4,653 t, respectively. Yield per recruit and biomass per recruit estimated were 2584.7 g and 3640.4 g. The exploitation rate, yield and yield per recruit indicate the stock of T. albacares to be overexploited. To sustainably and optimally harvest the species, reduction in the present fishing effort by 60 % is necessary, thereby increasing the yield by 35.31 %. With overcapacity in the number of gillnetters and hook and line units operating in the waters off Andhra Pradesh, implementation of the recommended fleet size proposed by Central Marine Fisheries Research Institute by means of buy-back measures and strict adherence to Minimum Legal Size at harvest would ensure sustainable and optimum exploitation of the resource. [ABSTRACT FROM AUTHOR]

Hyaluronic acid (HA) is commonly extracted from terrestrial and bacterial sources. However, due to the risk associated with animal and bacterial derived contaminants and the laborious production that tend to compromise the quality of HA, research studies have recently shifted to the exploration of HA in marine resources and the enhancement of the protocols for its production. In this study, isolation of HA from yellowfin tuna Thunnus albacares (Bonnaterre, 1788) eyeball was carried out through standardisation of the different steps in HA isolation, including tissue extraction, enzymatic hydrolysis, precipitation with cetylpiridinium chloride (CPC) - sodium chloride (NaCl) solution, filtration and diafiltration, precipitation in alkaline hydroalcoholic solution, and lyophilisation. Results revealed that the highest HA yield and purity were observed in the indirect treatment wherein the raw material was pre-treated with acetone, formaldehyde, and sodium acetate solution prior to water extraction and incubation of the mixture for 24 h. Enzymatic hydrolysis revealed that 6 h was the maximum tissue hydrolysis period. Results further showed that the optimum conditions for HA isolation were through the use of 3% CPC:3M NaCl concentration for recovery and fractionation and a 1:3 mL.mL-1 supernatant:ethanol ratio for alcoholic precipitation. The data gathered from this study could help maximise the benefits of the tuna processing waste in the country and may open a new opportunity for more cost-effective production of a valuable bioactive compound from a natural source. [ABSTRACT FROM AUTHOR]

Yellowfin tuna by-products (Thunnus albacares) were processed to produce radical-scavenging peptides from hydrolysis by lactic acid fermentation (LAF) with Lactobacillus plantarum, papaya fruit (Carica papaya), and molasses as a carbon source for 72 h. A 15-kDa peptide was purified; after de novo sequencing, it was determined that fragments are rich in hydrophobic and neutral amino acids. The results suggest this effect is mainly to the hydrophobicity of the amino acids in their sequence. Further work is on progress to assess the ability of peptides to provide stability in lipids or in other types of samples sensitive to the action of free radicals. [ABSTRACT FROM AUTHOR]

This dissertation addressed questions related to the selective advantages of regional endothermy and its independent evolution among several fish groups. The chapters integrate laboratory and field physiological measurements to provide a better understanding ofthe evolution of regional endothermy and its importance as an adaptation to the pelagic environment. Studies on the swimming energetics of the eastern Pacific bonito (Sarda chiliensis) revealed that the bonito had a similar cost of transport to that of similar sized yellowfin tuna (Thunnus albacares) and that the bonito had a significantly lower standard metabolic rate than tuna. Thermal studies on live slender tuna, Allothunnus fallai, were the first to document both red muscle (RM) and eye and brain endothermy for this species and establish regional endothermy as a synapomorphy of the Tribe Thunnini. Additional findings for A.fallai revealed this species to possess a brain heater, a thermogenic organ composed of portions of all four of the rectus muscles. These findings demonstrate that the use of a heater tissue to elevate cranial temperatures has evolved independently in at least three fish groups.Movement studies on the mako shark (Isurus oxyrinchus) used acoustic telemetry to record the in-vivo stomach temperatures and depth distribution off the coast of Southern California. This work showed that the tracked makos remained in the upper 12 m of the water column during up to 80% of the track period and that larger makos dove to greater depths. Stomach temperature data were recorded for all tracked makos (including young of the year), and these data were used to document feeding events in five of the seven tracking sessions.A study on the muscle morphology of three thresher shark species (common thresher, Alopias vulpinus, pelagic thresher, A. pelagicus, and bigeye thresher, A. superciliosus) revealed interspecific differences in the RM transverse position and distribution along the body. The common thresher is the only alopiid to possess a RM arrangement similar to that of the endothermic lamnid sharks, suggesting that this species is likely the only thresher to be capable of RM endothermy.

Recent studies argue for the presence of genetic population structure in yellowfin tuna (Thunnus albacares) in all oceans. However, the persistence of family groups has never been considered a viable mechanism of structure, nor has it been measured. We analyzed genetic similarity among 280 yellowfin tunas from seven population samples collected in the Western and Central Pacific Ocean (WCPO) using single nucleotide polymorphisms, and found population structure that was significantly explained by the presence of 96 individuals involved in 332 half or full sib dyads. We found significantly higher mean and median relatedness between individuals from the same sample groups, compared to individuals from different groups; and high relatedness between individuals caught at the same fish-aggregating device (FAD) than between those caught in the wider exclusive economic zone (EEZ). Alternatively, the EEZ of the Federated States of Micronesia may harbor exceptionally large numbers of close kin. We conclude that yellowfin directly school with related individuals through their first year, and at least demonstrate tightly overlapping regional fidelity as adults. These results may explain, to some extent, the patterns of population genetic structure recently observed in yellowfin tuna.

Introduction: Tuna has a cost-effective energy supply to support the regional endothermic and high-speed swimming performance. The gut symbiotic microbiotas and their metabolites play essential roles in tuna's diet digestion, absorption, and energy acquirement, which are often highly related to the ontogenetic development of tuna., Methods: We compared gut microbial compositions and metabolites, as well as mRNA expression of the intestine between juvenile and adult yellowfin tuna using 16S rRNA sequencing, metabolomic and transcriptomic, respectively., Results and Discussion: The results revealed that adults had a significantly higher microbial diversity and abundance of Acinetobacter than juveniles. Regarding the gut microbiota-derived metabolites, fatty acids, especially glycerophospholipid and sphingolipid, were significantly enriched in adults than in juveniles. Moreover, the short-chain fatty acid (butyrate and isobutyrate) contents were significantly higher in adults than in juveniles. To find the relationship between gut microbiotas and host physiology, intestinal transcriptome analysis demonstrated that the enriched pathways of differential expression genes (DEGs) in adult tuna were the lipid metabolism pathway, including "fat digestion and absorption," "cholesterol metabolism," "steroid hormone biosynthesis," "glycerolipid metabolism," and "glycerophospholipid metabolism." However, protein digestion and absorption and pancreatic secretion pathways were significantly enriched in the juveniles. The conjoint analysis indicated that the enriched pathways of both differential metabolites (DMs) and DEGs were remarkably related to the regulation of glycerophospholipids metabolism in adult tunas. This study highlights the role of gut microbiotas in fish nutrition metabolism. These findings provide new insights into the view of ontogenetic shifts of gut microbiotas and their metabolites on host health and gut function in endothermic and high-speed swimming marine fish species., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zou, Zhang, Wu, Lu, Xiao, Huang, Fu and Guo.)

This study was conducted to develop systems for the identification of four tuna species (skipjack tuna Katsuwonus pelamis, yellowfin tuna Thunnus albacares, bullet tuna Auxis sp. and Atlantic bonito Sarda sp). At first, raw samples of these species and a mix intended as internal control were prepared for the authentication of fish muscle tissue of the genus Thunnus sp., Auxis sp. and Sarda sp. DNA from raw muscle tissue, the mix and samples was extracted with the DNeasy mericon Food Kit (Qiagen GmbH, Hilden, Germany). The concentration and purity of DNA in raw samples were evaluated using a spectrophotometer. Primers and probe sequences were specifically designed to identify the selected species. In addition, primers and a probe for the endogenous 12S rRNA gene were designed to determine the presence of amplifiable fish (especially tuna) DNA in samples. Furthermore, the species specificity of the designed primers and probes was verified in DNA samples of various tuna and bonito species. Limit of detection for the selected species was calculated as well as the coefficient of determination R2 and efficiency of real-time PCR testing was determined. To evaluate the developed real-time PCR methods, 70 commercial tuna products were analysed. The results show that mislabelling of fish products can still be encountered and, moreover, the presence of an additional species can be identified.

Decades of study have attempted to describe the population genetic structure of tuna species, including a significant number of studies focused on yellowfin (Thunnus albacares). Very often, analyses do not agree on how many stocks exist per species, and where their boundaries lie. This is possibly because studies vary so much across numerous variables, such as the geographic range covered in a study, the number and type of genetic markers used, and the number of sites sampled. This meta-analysis of 22 yellowfin studies attempts to standardize and isolate the key variables to assessing the strength of correlation with the resulting number of populations a study observes. Overall trends across the studies suggest that genetic markers with a high probability of being under selective constraints, or are located in coding regions, are more or less likely to sense population structure depending on geographic range coverage. Alternatively, when assessing neutral or non-coding genomic regions, studies also benefit from (i) more polymorphic and numerous loci, and (ii) increasing the number of sampling locations analyzed, both of which increase the statistical power of an analysis. Finally, trends were clearest when groups of accurately identified coding and non-coding, or neutral and non-neutral, studies were further subdivided by whether they used mitochondrial or nuclear DNA, confirming that analyses of the two genomes should not be compared directly. Our meta-analysis provides concrete support for long held assumptions about the relationships between population genetics study parameters and outcomes, and provides guidance for future studies on how to maximize the likelihood of identifying population structure where it exists. [ABSTRACT FROM AUTHOR]

The most common methods that can be used for species identification of tuna include methods based on detection of species-specific DNA via the polymerase chain reaction (PCR) method. The problem with DNA detection in processed products is the possibility of DNA fragmentation during the technological process. The quantity and quality of extracted DNA is a crucial step for species identification based on the DNA analysis. In this study, two DNA extraction methods (DNeasy Blood & Tissue Kit and DNeasy mericon Food Kit) for tuna DNA isolation were compared. Eight food products of canned tuna (three of them were declared as Thunnus albacares and five products were declared as Katsuwonus pelamis) with a different addition of various ingredients were tested. Furthermore, three different times of proteolysis (30 min, 60 min, overnight) for each sample and each extraction kit were evaluated. The DNA concentration was determined by a Qubit dsDNA HS Assay Kit fluorescence method and quantified using a Qubit fluorometer. The DNA purity was evaluated using the A260/A280 ratio of absorbances measured on a spectrophotometer. The main indicator of DNA quality and quantity was its amplifiability in the subsequent real-time PCR for Thunnus species, Thunnus albacares and Katsuwonus pelamis. Based on the results, both kits can be used for tuna species determination in highly heat-treated products with different composition, nevertheless, the DNeasy mericon Food Kit provided better statistical values in some parameters. The effect of different times of proteolysis was significant in most of the samples with regard to the crossing point values determined by real-time PCR. [ABSTRACT FROM AUTHOR]

This research project was undertaken on two species of tropical tuna: the yellowfin Thunnus albacares and the skipjack Katsuwonus pelamis, to explore the dynamic physiological design features of tunas which underly the highly-developed thunniform propulsion mechanism. To achieve an integrated understanding of tuna swimming mechanics, a combination of electromyography, kinematics, and direct force measurements was used to probe the dynamic function of the myotomal red muscle as fish swam over a range of sustained, aerobic swimming speeds in a large water tunnel.In both species, onset of red muscle activation proceeds sequentially in a rostra-caudal direction, while deactivation is nearly simultaneous at all sites, coincident with peak force in the caudal tendons. In yellowfin, there is complete segregation of contralateral activity, while in skipjack there is slight overlap. In both species, all red muscle on one side is active simultaneously for part of each cycle. Comparison with other fish species shows that the tuna EMG patterns culminate a spectrum of activation patterns underlying swimming modes from anguilliform to thunniform.Coupling kinematics with EMG data revealed that muscle activation occurs after the midline has reached its maximum convexity at any given axial location; therefore, it was concluded that midline curvature is not an accurate indicator of local strain in the myotomal cone muscle. Activation and curvature waves travel down the body at the same rate, so muscle function is predicted not to vary by axial location. In yellowfin, peak force occurs in the caudal tendons as the tail tip crosses the midpoint of its sweep; in skipjack, when the tip is about three-quarters through its sweep.Internal force measurements were made for the first time in any fish species by fitting a stainless steel buckle force transducer around the pair of deep caudal tendons on one side. At cruising speeds, mean forces were 1.3-3.8 N in yellowfin and 1.6-4.1 N in skipjack. At restrained burst speeds, forces were approximately 10 times higher. The biomaterial properties of the tendons show that they function as inextensible linkages, rather than biological springs, in transferring muscle force to the tail.

Oxygen transport and cardiovascular responses to exercise and environmental changes were studied in swimming yellowfin tuna (Thunnus albacares). a high­ performance teleost. Use of a large water tunnel permitted measurements of heart rate (fH), relative stroke volume (SV), and arterial and venous blood-gas variables during increases in swimming velocity or rapid changes in ambient temperature and oxygen content that simulated vertical migrations through the thermocline.In addition. a model of aerobic swimming performance for a 50 cm yellowfin was developed, based on the oxygen delivery potential of the cardiovascular system, and oxygen demand during swimming. This model predicts a lower maximal sustained (aerobic) velocity (~3-4 fork lengths [FL]/s) compared to previous estimates. This model also predicts that the rate of total oxygen consumption is not maximal at the maximal sustained velocity, suggesting that additional aerobic scope is available for other high­ oxygen demand metabolic functions (e.g., digestion. oxygen debt recovery, and growth).Measured FHs, although highly variable among individuals, were generally lower than previously reported, averaging from 68 to 102 bpm with a velocity increase from 1.0 to 2.9 FL/s. These FHs are comparable to other active teleosts at similar velocities. A significant bradycardia developed at an oxygen tension of 16.1 kPa O2, and fH was very sensitive to changes in temperature (Q10 = 2.37, 28°C to l8°C).Relative SV measurements revealed a slight decrease with exercise (mean 3.9%), indicating that, unlike most other fishes, cardiac output in the yellowfin is regulated primarily through changes in fH. However, SV increased with a drop in temperature, moderating the effect of fH on cardiac output (Q10 = 1.52).Yellowfin blood has a high-oxygen carrying capacity (16 to 18 ml O2/dl) and a low in vivo oxygen affinity (P50 = 5.3 kPa). Exercise caused in rise in arterial (74 to 88%) and a decline in venous oxygen saturation (48 to 44%), increasing the arterial-venous oxygen content difference [(a-v)O2], but leaving a large venous oxygen reserve. It is estimated that cardiac output and (a-v)O2 contributed equally to the increased oxygen uptake.xviii

Authentication of tuna fish products is necessary to assure consumers of accurate labelling of food products. The quality of species specific DNA crucially affects the efficiency of amplification during the subsequent PCR. The problem in DNA detection in canned products lies in the possibility of the fragmentation of DNA during the processing technologies and the use of ingredients (oil, salt, spice), that may inhibit the PCR reaction. In this study three DNA extraction methods were compared: DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit and Chemagic DNA tissue 10 Kit. The quantity and quality of DNA were evaluated by measuring DNA concentration and ratios A260/A280. Several parameters were estimated: the effect of whole and mechanically treated muscle, sterilization procedure used in canned process (high temperature in combination with high pressure) and addition of raw materials. The highest DNA concentrations were observed in non-processed muscle that is not influenced by the sterilization process. Canned whole muscle demonstrated lower DNA yield, and furthermore, the mechanical treatment (canned ground) resulted in lower values of DNA concentration that was registered by using all three types of DNA extraction kits. DNeasy mericon Food Kit produced DNA of higher concentration in non-processed sample, Chemagic DNA tissue 10 Kit delivered higher DNA yields than kits DNeasy Blood and Tissue Kit and DNeasy mericon Food Kit in canned samples, although the purity was lower, but still within the range 1.7 - 2.0. DNA was considered to be satisfactorily pure in all three types of samples and using all three types of DNA isolation. In case of the samples enriched of ingredients and treated with sterilization process as whole or ground muscle Chemagic DNA tissue 10 Kit produced in all samples (whole and ground muscle) the highest values of DNA concentration, but almost all values of A260/A280 were lower than 1.7. Therefore DNeasy mericon Food Kit appears to be a favorite one, in all samples with whole muscle gives higher values of DNA concentrations than DNeasy Blood and Tissue Kit. Addition of ingredients influenced the DNA yield in terms of decreasing in samples containing vinegar and lemon, but some of the ingredients resulted surprisingly in higher yield of DNA. This was not consistent in whole and ground muscle, and the differences were described also among particular kits. The impact of ingredients was not conclusively approved and their importance to the suitability of extracted DNA for PCR amplification is needed to be discussed in further analysis.