78 results on '"T. Yutaka"'
Search Results
2. Epidemiology and characteristics of infant out-of-hospital cardiac arrest and factors associated with outcome
- Author
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Omatsu, Kentaro, primary, Inaba, Hideo, primary, KAMIKURA, Takahisa, primary, Owada, Hitoshi, primary, and T, Yutaka, primary
- Published
- 2022
- Full Text
- View/download PDF
3. Association of a bathroom with an adjacent dressing room with neurologically unfavourable outcomes of at-home out-of-hospital cardiac arrest: a propensity score matching analysis of a nation-wide cohort
- Author
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T, Yutaka, primary
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- 2022
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4. Delayed-Enhancement Magnetic Resonance Imaging Could Detect the Substrate of an Unusual Macroreentrant Atrial Tachycardia?
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Akira Shimane, Katsunori Okajima, Kunihiko Kiuchi, and R T Yutaka Shigenaga
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Tachycardia ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Substrate (chemistry) ,Magnetic resonance imaging ,Delayed enhancement ,Image enhancement ,Nuclear magnetic resonance ,Physiology (medical) ,Internal medicine ,Cardiology ,medicine ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Atrial tachycardia - Published
- 2014
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5. 931 A comparison of 90-day complications between ileal conduit and neobladder reconstruction after radical cystectomy: A multi-institutional retrospective study in Japan
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Haruo Seki, J. Ishizaki, K. Hirakawa, Nobuo Shinohara, M. Togashi, Takashige Abe, S. Kamota, Ryuji Matsumoto, G. Mouri, K. Minami, T. Yamashita, T. Seki, Yuichiro Shinno, Takahiro Osawa, T. Akino, T. Kaneda, T.A. Aoyagi, T. Mochizuki, Satoru Maruyama, S. Kanzaki, T. Yutaka, T. Shibata, T. Ono, N. Takada, K. Sugishita, N. Miyajima, H. Matsuda, H. Katano, Y. Kanno, T. Sato, Ataru Sazawa, S. Sato, T. Sakuta, H. Sano, and Katsuya Nonomura
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Cystectomy ,medicine.medical_specialty ,Electrical conduit ,business.industry ,Urology ,medicine.medical_treatment ,medicine ,Retrospective cohort study ,business ,Surgery - Published
- 2013
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6. A microprocessor with a 128 b CPU, 10 floating-point MACs, 4 floating-point dividers, and an MPEG2 decoder
- Author
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K. Kutaragi, M. Suzuoki, T. Hiroi, H. Magoshi, S. Okamoto, M. Oka, A. Ohba, Y. Yamamoto, M. Furuhashi, M. Tanaka, T. Yutaka, T. Okada, M. Nagamatsu, Y. Urakawa, M. Funyu, A. Kunimatsu, H. Goto, K. Hashimoto, N. Ide, H. Murakami, Y. Ohtaguro, and A. Aono
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- 2003
- Full Text
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7. Pathological study on a severe sialidosis (?-neuraminidase deficiency)
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W. Yoshihara, Y. Matsumoto, Shintaro Okada, T. Yutaka, Hyakuji Yabuuchi, Morimi Shimada, Koji Inui, K. Matsuzaki, and Tsunekazu Yamano
- Subjects
Male ,Nervous system ,Pathology ,medicine.medical_specialty ,Perforation (oil well) ,Myenteric Plexus ,Neuraminidase ,Biology ,Nervous System ,Pathology and Forensic Medicine ,Pathogenesis ,Cellular and Molecular Neuroscience ,medicine ,Humans ,Endothelium ,Sialidosis ,Myenteric plexus ,Cerebral Cortex ,Kidney ,Infant ,Anatomy ,medicine.disease ,Spinal cord ,Microscopy, Electron ,medicine.anatomical_structure ,Liver ,Spinal Cord ,nervous system ,Vacuoles ,Neurology (clinical) ,Paraganglion - Abstract
A 56-day-old infant with alpha-neuraminidase deficiency, whose clinical features included severe edema of extremities and ascites which resembled those in severe infantile sialidosis, was autopsied. Perforation, whose pathogenesis was unclear, was found on the descending portion of the duodenum. Light and electron microscope studies showed that neurons in the cerebral and cerebellar corticies, and the thoracic spinal cord contained membrane-bound vacuoles but no membranous cytoplasmic bodies. Zebra bodies were found only in the neurons of the spinal cord. The neurons in the paraganglion and in the Auerbach's myenteric plexus were also distended with numerous membrane-bound vacuoles. Hepatocytes, endothelial cells and Kupffer cells in the liver and glomerular and tubular epithelial cells in the kidney were swollen with a number of vacuoles, although the patient showed none of the clinical features of renal involvement. These pathological changes were similar to those in nephrosialidosis reported by Le Sec et al. [Arch Fr Pediatr 35:819-829 (1978)].
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- 1986
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8. A severe infantile sialidosis (?-galactosidase-?-neuraminidase deficiency) mimicking GM1-gangliosidosis type 1
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Tomochika Kato, T. Dezawa, T. Yutaka, T. Yamano, Michio Koike, Shintaro Okada, Sugino H, and Hyakuji Yabuuchi
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Pathology ,medicine.medical_specialty ,Urinary system ,Hepatosplenomegaly ,Neuraminidase ,macromolecular substances ,Diagnosis, Differential ,Lactose Intolerance ,Hydrolase ,medicine ,Humans ,Lymphocytes ,Sialidosis ,Gangliosidoses ,Kidney ,biology ,business.industry ,Infant ,Fibroblasts ,Hydrogen-Ion Concentration ,medicine.disease ,Phenotype ,Complementation ,medicine.anatomical_structure ,Pediatrics, Perinatology and Child Health ,biology.protein ,Female ,medicine.symptom ,business - Abstract
We observed a 3-month-old Japanese female infant with severe psychomotor retardation, coarse facial appearance, hepatosplenomegaly, and dysostosis multiplex. Only beta-galactosidase was found to be deficient when the routine lysosomal hydrolase assay was performed on the patient's lymphocytes at 6 months of age. At first GM1-gangliosidosis type 1 seemed the most likely diagnosis. Later, however, additional studies (hydrolase assay in cultured skin fibroblasts, urinary oligosaccharide analysis, genetic complementation study, etc.) revealed that biochemical data of this case were in agreement with those of severe infantile sialidosis. The only important exception was that alpha-neuraminidase in the patient's lymphocytes showed normal activity but abnormal pH dependence toward 4-methylumbellyferyl substrate. In addition, a severely damaged kidney suggested that his case may be classified as a unique type of severe infantile sialidosis (possible nephrosialidosis). These observations stress the importance of careful biochemical diagnosis of a case with GM1-gangliosidosis type 1 phenotype.
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- 1983
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9. Postoperative complications after adjuvant surgery for lung cancer
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K Daizo, G Tomoyuki, T Yutaka, W Hideyo, S Keiichi, N Hidenori, S Tetsuro, S Hideyuki, M Hideaki, K Haruhiko, T Ryosuke, and N Tsuguo
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medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Postoperative complication ,medicine.disease ,law.invention ,Surgery ,Randomized controlled trial ,law ,Toxicity ,medicine ,Non small cell ,Stage (cooking) ,Lung cancer ,business ,Adjuvant ,Neoadjuvant therapy - Abstract
Neoadjuvant therapy is a new modality treatment for primary lung cancer. This paper analyzes the complications after 30 adjuvant operations in our institution from Jan. 1984 to Dec. 1987. Thirty patients were treated with preoperative chemotherapy (two or more courses) in a randomized controlled study. The protocol was as follows : 1) CPA+ADM +VCR or CDDP+VP-16 therapy in 13 patients with small cell lung cancer. 2) CDDP+VDS or CDDP+VDS+MMC therapy in 17 patients with non-small cell lung cancer. The postoperative complication rate was 73.3% (22 of 30). The major complication rate was 16.7% (5 of 30). The overall operative and hospital mortality rate was 6.7% (2 of 30). Many patients with small cell lung cancer had side effects from preoperative radiotherapy or cardiac toxicity induced by Adriamycin. Many patients with non-small cell lung cancer had complications following resection for advanced disease (Stage III) or renal toxicity induced by CDDP.All patients had prolonged water retention, and some had late postoperative complications.
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- 1988
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10. A case of mucolipidosis II: Biochemical, nutritional, and immunological studies
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Kyungsae Ha, Osamu Nose, Hiroshi Kai, Hyakuji Yabuuchi, Shintaro Okada, Ikeda T, T. Yutaka, Shigeo Kojima, and Masahiro Kato
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Male ,Neuraminidase ,Physiology ,Biology ,Enzymatic Assays ,Developmental Neuroscience ,Mucolipidoses ,Normal basal metabolic rate ,medicine ,Humans ,Immunity, Cellular ,Growth retardation ,Mucolipidosis ,Respiratory infection ,General Medicine ,medicine.disease ,Diet ,Calorie intake ,Malnutrition ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Immunology ,biology.protein ,Basal Metabolism ,Neurology (clinical) ,Antibody ,Energy Intake ,Lysosomes - Abstract
A case of mucolipidosis II was studied biochemically, nutritionally and immunologically. A possible functional deficiency of T cells was observed, but discrepancy between B cells and immunoglobulin content was not reasonably explained at this moment. There was no basic nutritional problem in this case and it is more likely that his growth retardation was due to frequent episodes of severe respiratory infection because he received adequate calorie intake with low normal basal metabolic rate. Results of enzymatic assays were also presented.
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- 1979
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11. Studies on .ALPHA.-ketoglutaric aciduria in type I glycogenosis
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Koji Inui, Hiroko Kodama, Shintaro Okada, T. Yutaka, and Hyakuji Yabuuchi
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Male ,medicine.medical_specialty ,Adolescent ,Urinary system ,Alpha (ethology) ,Dehydrogenase ,Urine ,Glycogen Storage Disease Type I ,General Biochemistry, Genetics and Molecular Biology ,Excretion ,Glycogen Storage Disease Type III ,Urinary excretion ,Internal medicine ,medicine ,Humans ,In patient ,Citrates ,Child ,Pyruvates ,Chemistry ,General Medicine ,Citric acid cycle ,Glucose ,Endocrinology ,Child, Preschool ,Lactates ,Ketoglutaric Acids ,Female - Abstract
Urinary excretion of the organic acids in patients with type I and III glycogenosis was investigated. In all patients with type I glycogenosis, urinary alpha-ketoglutarate concentration ws about 10 times the normal value. alpha-Ketoglutaric aciduria was not improved by the acute or prolonged administration of a large dose of factors for pyruvate- and alpha-ketoglutarate dehydrogenase complex. On the other hand, the level of alpha-ketoglutarate in the urine from type I patients decreased in conjunction with the decrease of plasma lactate and pyruvate concentration after repeated oral glucose loading. Oral citrate loading brought an increased excretion of alpha-ketoglutarate in type I glycogenosis. It is possible that alpha-ketoglutarate dehydrogenase in the rate-limiting step in tricarboxylic acid cycle and in patients with glycogenosis type I, the excessive excretion of alpha-ketoglutarate may be caused by the limited activity of alpha-ketoglutarate dehydrogenase with excessive substrate.
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- 1980
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12. Ultrastructural study on nervous system of fetus with GM1-gangliosidosis type 1
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T. Yutaka, Shintaro Okada, Koji Inui, Tsunekazu Yamano, S. Kanzaki, Hyakuji Yabuuchi, Morimi Shimada, Tomochika Kato, and S. Kanda
- Subjects
Nervous system ,Pathology ,medicine.medical_specialty ,Cerebellum ,Central nervous system ,Myenteric Plexus ,G(M1) Ganglioside ,Biology ,Nervous System ,Pathology and Forensic Medicine ,Cellular and Molecular Neuroscience ,Fetus ,Parietal Lobe ,medicine ,Humans ,Gangliosidoses ,Myenteric plexus ,Cerebral Cortex ,Anatomy ,Spinal cord ,Microscopy, Electron ,medicine.anatomical_structure ,Spinal Cord ,nervous system ,Cerebral cortex ,Peripheral nervous system ,Vacuoles ,Radial Nerve ,Neurology (clinical) ,Neuroanatomy - Abstract
The nervous system of a 22-year-old fetus with GM1-gangliosidosis type 1 was studied by electron microscopy. The tissues thus examined were the cerebral cortex at the parietal region, the cerebellum, the thoracic spinal cord, the Auerbach's myenteric plexus in the large intestine and the radial nerve fibers. In the cerebral cortex, membrane-bound vacuoles, which occasionally contained stacks of fine fibrils, were observed in the large young neurons in the deeper part of the cortical plate. The neurons in the other part of the cerebral cortex carried no storage materials. In the cerebellum, the membrane-bound vacuoles with stacks of fine fibrils were seen only in the Purkinje cells. The neurons in the spinal cord also contained several zebra-like bodies and the above membrane-bound vacuoles. As for the peripheral nervous system (PNS), neurons in the Auerbach's myenteric plexus carried membranous cytoplasmic bodies and zebra-like bodies. Some of the axons in the radial nerve fibers also contained a lot of pleomorphic electron-dense bodies and a few membranous cytoplasmic ones. These results show that the accumulation of storage materials is started in the large neurons which are produced in the early stage of neurogenesis in the central nervous system (CNS). Additionally, the observed membrane-bound vacuoles are considered to be structures which occur before the membranous cytoplasmic bodies and/or the zebra-like bodies. It is also elucidated that the PNS is affected earlier than the cerebral and cerebellar cortices and thoracic spinal cord.
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- 1983
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13. Ultrastructural study of biopsy specimens of rectal mucosa. Its use in neuronal storage diseases
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T, Yamano, M, Shimada, S, Okada, T, Yutaka, T, Kato, and H, Yabuuchi
- Subjects
Niemann-Pick Diseases ,Tay-Sachs Disease ,Adolescent ,Brain Diseases, Metabolic ,Biopsy ,Plasma Cells ,Rectum ,Infant ,Sandhoff Disease ,G(M1) Ganglioside ,Leukodystrophy, Metachromatic ,Axons ,Microscopy, Electron ,Child, Preschool ,Humans ,Lymphocytes ,Intestinal Mucosa - Abstract
Rectal mucosa biopsy specimens from patients with neuronal storage diseases were examined by electron microscopy. The diseases were Tay-Sachs disease, Sandhoff's disease, Niemann-Pick disease types B and C, late infantile metachromatic leukodystrophy, GM1 gangliosidosis type 1, beta-galactosidase-neuraminidase deficiency, I-cell disease, and mucopolysaccharidoses (Hunter's syndrome and Sanfilippo's syndrome type A). Unmyelinated nerve fibers, endothelial cells, fibroblasts, plasma cells, and histiocytes were seen in the specimens. Except for plasma cells, the results thus obtained for various cells were similar to those obtained from skin and conjunctival biopsy specimens, which have been already reported. There has been no report so far on ultrastructure of the plasma cell in these diseases. Storage materials, eg, dense bodies and membrane-bound vacuoles, were observed in the plasma cells in various storage diseases, with the exception of late infantile metachromatic leukodystrophy. Thus, electron microscopy of rectal mucosa is useful in making diagnoses and examining plasma cells in some neuronal storage diseases.
- Published
- 1982
14. A case of neuraminidase deficiency associated with a partial beta-galactosidase defect. Clinical, biochemical and radiological studies
- Author
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Shintaro Okada, Hideaki Chiyo, T. Yutaka, Tomochika Kato, Mariko Inui, Chiiko Ikehara, Motomi Okawa, and Hyakuji Yabuuchi
- Subjects
medicine.medical_specialty ,Urinary system ,Neuraminidase ,Urine ,Lactose Intolerance ,Mucolipidoses ,Mucolipidosis I ,Internal medicine ,medicine ,Leukocytes ,Humans ,Child ,Cells, Cultured ,Craniofacial dysmorphism ,biology ,business.industry ,Fibroblasts ,Neuraminidase deficiency ,Fetuin ,Glycopeptide ,Enzyme assay ,Endocrinology ,Pediatrics, Perinatology and Child Health ,Immunology ,biology.protein ,Female ,business - Abstract
Neuraminidase deficiency towards fetuin, 2 leads to 3 sialyllactose and 2 leads to 6 sialyllactose was found in cultured skin fibroblasts from a 10-year-old Japanese girl who exhibits craniofacial dysmorphism, a short neck, vertebral and pelvic deformities and macular cherry-red spots. Neuraminidase deficiency in this case seems the primary enzyme defect because the enzyme activity of her parents was intermediate. In addition, beta-galactosidase in leukocytes and cultured skin fibroblasts from the patient was found to be severely deficient, but could be detected in serum and urine. In the parents, beta-galactosidase activity was normal. There were moderately increased levels of urinary sialic acid-rich oligosaccharides and glycopeptides in the patient. The clinical and biochemical observations suggest that this case is very close to mucolipidosis I.
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- 1979
15. Electron microscopic examination of skin and conjunctival biopsy specimens in neuronal storage diseases
- Author
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Shintaro Okada, T. Yutaka, Tsunekazu Yamano, Hyakuji Yabuuchi, Morimi Shimada, and Yuzo Nakao
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Pathology ,medicine.medical_specialty ,Conjunctiva ,Adolescent ,Biopsy ,Disease ,Developmental Neuroscience ,Mucolipidoses ,Medicine ,Humans ,Child ,Skin ,Inclusion Bodies ,Neurons ,Niemann-Pick Diseases ,Tay-Sachs Disease ,medicine.diagnostic_test ,business.industry ,Mucolipidosis ,Tay-Sachs disease ,nutritional and metabolic diseases ,Infant ,General Medicine ,Mucopolysaccharidoses ,medicine.disease ,Axons ,Microscopy, Electron ,medicine.anatomical_structure ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Skin biopsy ,Adrenoleukodystrophy ,Neurology (clinical) ,business ,Niemann–Pick disease ,Metabolism, Inborn Errors - Abstract
Skin and conjunctival biopsy specimens from fourteen patients with neuronal storage diseases were investigated using an electron microscope. The diseases were Tay-Sachs disease, ceroid-lipofuscinosis (Jansky-Bielschowsky type), Niemann-Pick disease (type B), highly suspected adrenoleukodystrophy, I-cell disease, mucolipidosis of the beta-galactosidase deficient type, Hurler disease, Hunter disease and Morquio disease. This examination provided valuable diagnostic information on some neuronal storage diseases but not on Morquio disease or highly suspected adrenoleukodystrophy. False negative results may sometimes occur using this examination method. However, this examination suggests the usefulness of skin and conjunctival biopsy specimens as a diagnostic tool in some neuronal storage diseases.
- Published
- 1979
16. Diagnosis of Tay-Sachs disease by estimation of beta-N-acetylhexosaminidase activity using a radiolabeled hyaluronic acid-derived trisaccharide substrate
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M. Midorikawa, Tomochika Kato, Hyakuji Yabuuchi, Shintaro Okada, T. Yutaka, and M Doke
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Clinical Biochemistry ,Biochemistry ,Isozyme ,Chromatography, DEAE-Cellulose ,Substrate Specificity ,chemistry.chemical_compound ,Hyaluronic acid ,medicine ,Humans ,Trisaccharide ,Cells, Cultured ,chemistry.chemical_classification ,Tay-Sachs Disease ,Chemistry ,Catabolism ,Biochemistry (medical) ,Tay-Sachs disease ,Substrate (chemistry) ,General Medicine ,Clinical Enzyme Tests ,Fibroblasts ,medicine.disease ,beta-N-Acetylhexosaminidases ,Acetylglucosamine ,Isoenzymes ,Enzyme ,Hexosaminidases ,Liver ,Trisaccharides - Abstract
We have prepared a new radiolabeled substrate (N-[3H]acetylglucosamine-glucuronic acid-N-[3H]acetylglucosamine), from hyaluronic acid, for an assay of beta-N-acetylhexosaminidase activity. Using this substrate, we found a striking deficiency of beta-N-acetylhexosaminidase activity in cultured skin fibroblasts and in liver homogenates from patients with Tay-Sachs disease. DEAE-cellulose chromatography at pH 6.0 revealed that both isoenzymes A and B of beta-N-acetylhexosaminidase from normal liver participated in the catabolism of hyaluronic acid. There were, however, major differences in substrate specificities between isoenzymes A and B. Our results indicate that this substrate should be useful for enzymatic diagnosis of Tay-Sachs disease.
- Published
- 1984
17. Induction of beta-galactosidase in beta-galactosidase-alpha-neuraminidase deficiency: effects of leupeptin and sucrose
- Author
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T, Kato, S, Okada, T, Yutaka, and H, Yabuuchi
- Subjects
Sucrose ,Hydrolases ,Leupeptins ,Neuraminidase ,Fibroblasts ,beta-Galactosidase ,Cathepsins ,Cathepsin B ,Cell Line ,Galactosidases ,Lactose Intolerance ,Enzyme Induction ,Humans ,Lysosomes ,Oligopeptides - Abstract
beta-Galactosidase was normalized by a serine-thiol protease inhibitor, leupeptin with concentration of 10 micrograms/ml in cultured skin fibroblasts from patients with beta-galactosidase-alpha-neuraminidase deficiency (beta-Gal-/Neu-). The induction of this enzyme was not observed in normal cells. Because the enzymic activity of cathepsin B1 increased significantly both in beta-Gal-/Neu- and normal cells by leupeptin loading, the restoration of beta-galactosidase in beta-Gal-/Neu- cells can not be explained by the theory that leupeptin inhibited intracellular degradation of beta-galactosidase molecules. The effects of leupeptin and sucrose on lysosomal hydrolase induction were compared.
- Published
- 1983
18. [A case of Fabry's disease without angiokeratoma: clinical, biochemical and electron microscopic study]
- Author
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K, Sumi, N, Ito, M, Takahashi, S, Hashimoto, J, Abe, S, Okada, E, Kadota, T, Nagaura, T, Yutaka, and H, Yabuuchi
- Subjects
Male ,Spinal Nerves ,Adolescent ,Muscles ,Fabry Disease ,Humans - Published
- 1986
19. Diagnosis of Tay-Sachs disease using radiolabelled chondroitin 6-sulphate-derived trisaccharides
- Author
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Tomochika Kato, M. Midorikawa, Shintaro Okada, T. Yutaka, and Hyakuji Yabuuchi
- Subjects
alpha-L-Fucosidase ,Tay-Sachs Disease ,Chemistry ,Tay-Sachs disease ,Chondroitin Sulfates ,Oligosaccharides ,medicine.disease ,Tritium ,Molecular biology ,chemistry.chemical_compound ,Biochemistry ,Liver ,Genetics ,medicine ,Chondroitin ,Humans ,Trisaccharides ,Genetics (clinical) ,Chondroitin Sulfate C - Abstract
Description d'une methode simple de preparation d'un substrat derive de la chondroitine 6 sulphate et de quelques proprietes enzymatiques de la BN acetyl hexoaminidase hepatique dans la maladie de Tay Sachs. L'auteur conclu que le substrat convient pour mesurer l'activite de l'hexoaminidase A sans avoir besoin de separer les isoenzymes, et serait donc utile pour le diagnostic de maladie de Tay Sachs
- Published
- 1983
20. [A case of infantile sialidosis associated with congenital chylous ascites]
- Author
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K, Matsuzaki, Y, Matsumoto, W, Yoshihara, S, Okada, M, Midorikawa, K, Inui, T, Yutaka, and T, Yamano
- Subjects
Male ,Infant, Newborn ,Humans ,Neuraminidase ,Peritonitis ,Chylous Ascites - Published
- 1987
21. Chromatographic study of serum hexosaminidase in normal and GM 2-gangliosidosis
- Author
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Kiyoomi Sumi, Hyakuji Yabuuchi, Shintaro Okada, and T. Yutaka
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Glucosamine ,Heterozygote ,Chromatography ,Hot Temperature ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Heterozygote advantage ,General Medicine ,Brain tissue ,Gangliosidosis ,medicine.disease ,Lipidoses ,Biochemistry ,Chromatography, DEAE-Cellulose ,Hexosaminidases ,Drug Stability ,Pregnancy ,medicine ,Humans ,Hexosaminidase ,Female - Abstract
β- N -Acetylhexosaminidase in normal human serum was separated into four components by DEAE-cellulose chromatography. The first three peaks were all heat stable. Neither conversion from heat-labile to heat-stable components, nor mutual conversion among heat-stable components occurred by heat treatment of serum. Serum hexosaminidase of Tay—Sachs disease patients had a high level of heat-stable components, especially of I 1 and I 2 , and this was similar to that of brain tissue. The activity of each component of a heterozygote of Tay—Sachs disease and Sandhoff's disease was intermediate between the normal and patient levels.
- Published
- 1974
22. Assay of glucocerebrosidase using a fluorescent analogue of glucocerebroside for the diagnosis of Gaucher disease
- Author
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M, Midorikawa, S, Okada, T, Yutaka, H, Yabuuchi, M, Naoi, K, Kiuchi, and K, Yagi
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Dansyl Compounds ,Male ,Gaucher Disease ,Genetic Carrier Screening ,Homozygote ,Infant ,Clinical Enzyme Tests ,Glucosylceramides ,Kinetics ,Spectrometry, Fluorescence ,Glucosylceramidase ,Humans ,Female ,Lymphocytes ,Glucosidases ,Fluorescent Dyes - Abstract
For the diagnosis of homozygotes and heterozygotes of Gaucher disease, glucocerebrosidase (glucocerebroside beta-D-glucoside glucohydrolase, EC 3.2.1.45) activity in lymphocytes was measured using a fluorescent analogue of glucocerebroside, 1-0-glucosyl-2-N-(dimethylaminonaphthalene-5-sulfonyl)-sphingosine as substrate. The activity in lymphocytes from homozygotes of Gaucher disease was found to be reduced markedly. This method is proved to be simple, sensitive, and specific as an assay of glucocerebrosidase activity for the diagnosis of Gaucher disease.
- Published
- 1985
23. Application of epithelial cells in culture to the biochemical studies of lysosomal hydrolase deficiencies
- Author
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Tomochika Kato, T Yamano, Shintaro Okada, T. Yutaka, and Hyakuji Yabuuchi
- Subjects
chemistry.chemical_classification ,Male ,Primary culture ,Cultured skin ,Hydrolases ,Biochemistry (medical) ,Clinical Biochemistry ,General Medicine ,Biology ,Trypsin ,Biochemistry ,Epithelium ,Cell biology ,Enzyme ,chemistry ,Hydrolase ,medicine ,Humans ,Female ,Lysosomes ,Cells, Cultured ,medicine.drug - Abstract
Epithelial cells are readily obtained when the primary culture of skin fibroblasts is established. The major lysosomal hydrolase activities in normal epithelial cells are lower than those in normal fibroblasts. The primary deficiency of lysosomal enzymes can be detected both in epithelial cells and in cultured skin fibroblasts. However, epithelial cells of I-cell disease show only beta-galactosidase deficiency. This result indicates that epithelial cells have different biochemical properties from skin fibroblasts and they may be similar to those of visceral organs. Therefore, cultured epithelial cells seem to be useful for the studies on lysosomal hydrolase deficiencies.
- Published
- 1983
24. Purification and some properties of human liver iduronate sulfatase
- Author
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T, Yutaka, A L, Fluharty, R L, Stevens, and H, Kihara
- Subjects
Chemistry ,Chromatography ,Chemical Phenomena ,Liver ,Solubility ,Humans ,Electrophoresis, Polyacrylamide Gel ,Iduronate Sulfatase ,Isoelectric Focusing ,Sulfatases - Abstract
Iduronate sulfatase was purified from human liver for an investigation of the degradative pathway of dermatan sulfate. An overall 80-fold purification was achieved and, more importantly, the preparation was free of alpha-L-iduronidase, beta-glucuronidase, N-acetylgalactosamine 4-sulfate sulfatase (arylsulfatase B) and highly enriched in beta-N-acetylhexosaminidase. The liver enzyme appeared to be composed of several molecular species. The enzyme activity was optimal at pH 4.0 and its Km was 10--20 microM with sulfoiduronyl sulfoanhydromannitol. Chloride was inhibitory at high concentration and among divalent metal ions, only copper was inhibitory. Nitrocatechol sulfate was not a substrate, but did show competitive inhibition. Its Ki for iduronate sulfatase was similar to its Km for arylsulfatase, suggesting a similarity in the substrate binding sites of iduronate sulfatase and arylsulfatases.
- Published
- 1982
25. Postoperative complications after adjuvant surgery for lung cancer
- Author
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M., Hideaki, primary, N., Tsuguo, additional, K., Daizo, additional, K., Haruhiko, additional, S., Hideyuki, additional, S., Tetsuro, additional, T., Yutaka, additional, N., Hidenori, additional, W., Hideyo, additional, G., Tomoyuki, additional, T., Ryosuke, additional, and S., Keiichi, additional
- Published
- 1988
- Full Text
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26. Hexosaminidase a activity in skin fibroblasts from various types of GM 2 gangliosidosis using a fluorogenic sulphated substrate
- Author
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Koji Inui, Robert J. Desnick, Hyakuji Yabuuchi, T. Yutaka, Shintaro Okada, and D. A. Wenger
- Subjects
Chemistry ,Tay-Sachs disease ,Substrate (chemistry) ,G(M2) Ganglioside ,Biological activity ,Fibroblasts ,Sandhoff disease ,Gangliosidosis ,medicine.disease ,Isozyme ,Catalysis ,beta-N-Acetylhexosaminidases ,Cell Line ,Hexosaminidase A ,medicine.anatomical_structure ,Biochemistry ,Genetics ,medicine ,Humans ,Hexosaminidase ,Gangliosidoses ,Fibroblast ,Hymecromone ,Genetics (clinical) ,Skin - Published
- 1985
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27. Clinical implications of NUP98::NSD1 fusion at diagnosis in adult FLT3-ITD positive AML.
- Author
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Miyajima T, Onozawa M, Yoshida S, Miyashita N, Kimura H, Takahashi S, Yokoyama S, Matsukawa T, Goto H, Sugita J, Fujisawa S, Hidaka D, Ogasawara R, Mori A, Matsuoka S, Shigematsu A, Wakasa K, Kasahara I, Saga T, Hashiguchi J, Takeda Y, Ibata M, Yutaka T, Fujimoto K, Kondo T, and Teshima T
- Subjects
- Child, Humans, Adult, Retrospective Studies, Prognosis, Mutation, fms-Like Tyrosine Kinase 3 genetics, Histone-Lysine N-Methyltransferase genetics, Leukemia, Myeloid, Acute diagnosis, Leukemia, Myeloid, Acute genetics, Leukemia, Myeloid, Acute therapy
- Abstract
Objectives: The cryptic fusion oncogene NUP98::NSD1 is known to be associated with FLT3-ITD mutation in acute myeloid leukemia (AML), and an independent poor prognostic factor in pediatric AML. However, there are little data regarding the clinical significance of NUP98::NSD1 in adult cohort., Methods: We conducted a multicenter retrospective study to investigate the prevalence, clinical characteristics, and prognostic impact of NUP98::NSD1 in adult FLT3-ITD-positive AML patients., Results: In a total of 97 FLT3-ITD-positive AML patients, six cases (6.2%) were found to harbor the NUP98::NSD1 fusion transcript. NUP98::NSD1 positive cases had significantly higher platelet counts and a higher frequency of FAB-M4 morphology than NUP98::NSD1 negative cases. NUP98::NSD1 was found to be mutually exclusive with NPM1 mutation, and was accompanied by the WT1 mutation in three of the six cases. The presence of NUP98::NSD1 fusion at the time of diagnosis predicted poor response to cytarabine-anthracycline-based intensive induction chemotherapy (induction failure rate: 83% vs. 36%, p = .038). Five of the six cases with NUP98::NSD1 underwent allogeneic hematopoietic stem cell transplantation (HSCT). Two of the five cases have successfully maintained remission, with one of them being rescued through a second HSCT., Conclusions: Detecting NUP98::NSD1 in adult FLT3-ITD-positive AML is crucial to recognizing chemotherapy-resistant group., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2023
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28. Predictive risk score of respiratory complications in children with mediastinal tumors: A case-control study.
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Honda M, Yuki A, Takahiro H, Chigusa O, Yuichi M, Makiko M, Fukuoka K, Koichi O, Yutaka T, Tetsuya I, Hiroshi K, Koichi M, Ikuya U, Norifumi K, and Katsuyoshi K
- Subjects
- Adolescent, Humans, Child, Case-Control Studies, Retrospective Studies, Respiratory Sounds, Cough, Risk Factors, Dyspnea complications, Hypoxia complications, Mediastinal Neoplasms complications, Mediastinal Neoplasms diagnostic imaging, Superior Vena Cava Syndrome complications
- Abstract
Background: The aim of this study was to examine risk factors of respiratory complications at the diagnosis and establish an algorithm of clinical management in children and adolescents with mediastinal tumors., Methods: We retrospectively collected clinical information of all children and adolescents who presented with mediastinal tumors at Saitama Children's Medical Center from 1999 to 2019, including age, sex, pathological diagnosis, eight major clinical symptoms (cough, dyspnea, hypoxia, orthopnea, chest pain, wheeze, superior vena cava syndrome, and stridor), chest computed tomography (CT) findings (tumor location, mediastinal mass ratio, pleural fluid, pericardial effusion, and compression of trachea and bronchi), types of diagnostic procedure and anesthesia, respiratory complications (severe hypoxia, difficult ventilation, respiratory failure, and cardiopulmonary arrest), and clinical outcome. Subsequently, we calculated the risk score for predicting respiratory complications by combining clinical and radiological findings., Results: Of the 57 patients, 7 (12%) developed respiratory complications. Cough, dyspnea, hypoxia, and orthopnea were significantly more common in patients with complications (p = 0.02, p = 0.02, p < 0.01, p = 0.03, respectively). The reduction of percentage of tracheal cross-sectional area (%TCA) and compression of the carina in chest CT were also significantly more common in patients with complications (p < 0.01 and <0.01, respectively). We calculated the risk score of respiratory complications by combining cough, wheeze, stridor, orthopnea, dyspnea, hypoxia, %TCA < 0.5, and compression of the carina. A risk score ≥ 7 showed high predictive accuracy for complications (sensitivity: 100%, specificity: 97.7%, positive likelihood ratio: 43.0)., Conclusion: The risk score combining clinical symptoms with radiological findings is a promising predictive tool for respiratory complications in children with mediastinal tumors., (© 2022 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
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- 2023
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29. A case of acute focal bacterial nephritis with acute kidney injury presenting as acute abdomen.
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Kishimoto N, Mori Y, Yutaka T, Oishi E, and Morita T
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- Acute Disease, Adult, Child, Female, Humans, Middle Aged, Abdomen, Acute diagnosis, Abdomen, Acute etiology, Acute Kidney Injury diagnosis, Acute Kidney Injury etiology, Acute Kidney Injury therapy, Nephritis complications, Pyelonephritis complications, Pyelonephritis diagnosis, Pyelonephritis microbiology
- Abstract
Acute focal bacterial nephritis (AFBN) refers to the bacterial infection of the renal parenchyma without abscess formation. Although AFBN has mainly been reported in pediatric patients, it may be underdiagnosed in adults as it resembles acute pyelonephritis in its clinical presentation. However, the symptoms suggesting acute abdomen is an important clue to diagnose AFBN, which requires additional imaging studies such as contrast-enhanced computed tomography (CECT). Here, we present the case of a 49-year-old female presenting to our emergency room with acute abdomen as well as acute kidney injury (AKI). CECT was performed to rule out critical etiologies of severe abdominal pain and the results revealed multifocal wedge-shaped shadows in the right kidney and diffuse enlargement of bilateral kidneys. We diagnosed the patient with AFBN and treated her through temporal hemodialysis (two sessions) and antibiotics for 23 days. Although AKI associated with AFBN has rarely been reported, her renal dysfunction and other symptoms were completely improved. In conclusion, clinicians should be aware of AFBN and be cautious to avoid the unnecessary invasive interventions., (© 2022. The Author(s) under exclusive licence to The Japan Society of Nephrology.)
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- 2022
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30. Oncologic feasibility of D1+ gastrectomy for patients with cT1N1, cT2N0-1, or cT3N0 gastric cancer.
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Kota I, Makoto H, Satoshi K, Yutaka T, Etsuro B, and Masanori T
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- Adult, Aged, Aged, 80 and over, Feasibility Studies, Female, Humans, Japan epidemiology, Lymphatic Metastasis, Male, Middle Aged, Prognosis, Propensity Score, Retrospective Studies, Stomach Neoplasms mortality, Stomach Neoplasms secondary, Survival Rate trends, Treatment Outcome, Gastrectomy methods, Lymph Nodes pathology, Neoplasm Staging, Stomach Neoplasms surgery
- Abstract
Introduction: D2 gastrectomy has shown a survival benefit in patients with highly advanced gastric cancer; however, it remains unclear whether D2 gastrectomy is required for patients with early-stage advanced gastric cancer or early gastric cancer with limited lymph node metastasis. This analysis aimed to clarify the oncologic feasibility of D1+ gastrectomy in patients with cT1N1, cT2N0-1, or cT3N0 gastric cancer., Methods: This retrospective cohort analysis included 466 patients with cT1N1, cT2N0-1, or cT3N0 gastric cancer who received curative gastrectomy with either D2 or D1+ dissection. Surgical outcomes were compared between the D2 group (n = 406) and the D1+ group (n = 60)., Results: The number of patients with higher age and higher comorbidity index was greater in the D1+ group than in the D2 group. Postoperative complications were significantly lower in the D1+ group than in the D2 group (10.0% vs. 26.8%, p = 0.004). No statistically significant difference in 5-year overall survival (p = 0.146) and disease-specific survival (p = 0.807) between the groups was noted. The incidence of local recurrences (p = 0.500) and that of lymph node recurrences (p = 1.000) were also similar between the groups. Multivariable analysis for overall survival identified age, clinical node-positive status, high Charlson score (≥3), advanced pathological stage (≥III), and postoperative complication (grade ≥ II) as independent prognostic factors. The propensity score-matched analysis showed very similar survival outcomes between the groups., Conclusion: D1+ gastrectomy may be oncologically feasible for patients with cT1N1, cT2N0-1, or cT3N0 stage gastric cancer., Competing Interests: Declaration of competing interest Drs. Itamoto Kota, Hikage Makoto, Kamiya Satoshi, Tanizawa Yutaka, and Bando Etsuro have no conflicts of interest or financial ties to disclose. Dr. Terashima Masanori has received personal fees from Taiho, Chugai, Ono, Bristol-Myers Squibb, Yakult, Takeda, Eli Lilly, Pfizer and Daiichi Sankyo, outside the submitted work., (Copyright © 2020 Elsevier Ltd, BASO ~ The Association for Cancer Surgery, and the European Society of Surgical Oncology. All rights reserved.)
- Published
- 2021
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31. Ultra-high level of serum soluble interleukin-2 receptor at diagnosis predicts poor outcome for angioimmunoblastic T-cell lymphoma.
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Shiratori S, Kosugi-Kanaya M, Shigematsu A, Kobayashi H, Yamamoto S, Kobayashi N, Iwasaki H, Mori A, Kunieda Y, Yutaka T, Kurosawa M, Kakinoki Y, Endo T, Kondo T, Hashino S, and Teshima T
- Subjects
- Aged, Aged, 80 and over, Cyclophosphamide administration & dosage, Disease-Free Survival, Doxorubicin administration & dosage, Female, Follow-Up Studies, Humans, Immunoblastic Lymphadenopathy blood, Immunoblastic Lymphadenopathy diagnosis, Lymphoma, T-Cell blood, Lymphoma, T-Cell diagnosis, Male, Middle Aged, Multivariate Analysis, Predictive Value of Tests, Prednisolone administration & dosage, Prognosis, Retrospective Studies, Solubility, Treatment Outcome, Vincristine administration & dosage, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Biomarkers, Tumor blood, Immunoblastic Lymphadenopathy drug therapy, Lymphoma, T-Cell drug therapy, Receptors, Interleukin-2 blood
- Abstract
Angioimmunoblastic T-cell lymphoma (AITL) is a rare subtype of non-Hodgkin lymphoma and displays an aggressive clinical course with poor outcome. To identify prognostic factors for AITL, we retrospectively analyzed 36 patients with AITL. The median age was 74 years with 83% of the patients having advanced stage. Eighty-three percent received CHOP (cyclophosphamide, doxorubicin, vincristine, prednisolone)-like chemotherapies, resulting in an overall response rate of 63%. With a median follow-up of 9 years, the estimated overall survival at 5 years was 33.3%. Median serum level of soluble interleukin-2 receptor (sIL-2R) was 5615 U/mL at diagnosis, and over 10 000 U/mL of sIL-2R was identified as a significant poor prognostic factor, independent of the International Prognostic Index, Prognostic Index for peripheral T-cell lymphoma and Prognostic index for AITL (hazard ratio [HR], 4.42; 95% confidence interval [CI], 1.49-13.11; log-rank, p < 0.01). Our study shows that an ultra-high level of serum sIL-2R at diagnosis is a significant poor prognostic biomarker for AITL.
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- 2015
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32. Sustained CD4 and CD8 lymphopenia after rituximab maintenance therapy following bendamustine and rituximab combination therapy for lymphoma.
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Yutaka T, Ito S, Ohigashi H, Naohiro M, Shimono J, Souichi S, and Teshima T
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- Antineoplastic Combined Chemotherapy Protocols adverse effects, Bendamustine Hydrochloride administration & dosage, Humans, Lymphocyte Count, Lymphopenia blood, Lymphopenia diagnosis, Maintenance Chemotherapy, Rituximab administration & dosage, Rituximab adverse effects, Antineoplastic Combined Chemotherapy Protocols therapeutic use, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Lymphoma complications, Lymphoma drug therapy, Lymphopenia etiology, Rituximab therapeutic use
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- 2015
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33. Genome sequence of a novel HIV-1 circulating recombinant form (CRF57_BC) identified from Yunnan, China.
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Wei H, Liu Y, Feng Y, Hsi J, Xing H, He X, Liao L, Yutaka T, Li J, and Shao Y
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- Adolescent, Adult, China, Cluster Analysis, Evolution, Molecular, Female, Genotype, HIV-1 isolation & purification, Humans, Male, Molecular Sequence Data, Phylogeny, RNA Viruses isolation & purification, RNA, Viral genetics, Young Adult, Genome, Viral, HIV Infections virology, HIV-1 classification, HIV-1 genetics, RNA Viruses genetics, Recombination, Genetic, Sequence Analysis, DNA
- Abstract
We identified a novel HIV-1 circulating recombinant form (designated CRF57_BC) from a total of four patients with no obvious epidemiologic linkage in western Yunnan (Dehong prefecture) in China. Two strains (09CN.YNFL37 and 10CN.DHFL17) were identified in this study. An additional two strains (341 and 1439) were found among strains reported in a previous study. CRF57_BC was composed of subtype B and subtype C, with one subtype B segment inserted into the gag region of the subtype C backbone. Subregion tree analysis showed that the B regions originated from a Thai B lineage and the C regions were from an India C lineage. The emergence of CRF57_BC may reflect the continual generation of various forms of intersubtype recombinants in western Yunnan.
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- 2014
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34. Synthesis of pendant-type anthraquinone-bridged cofacial dinuclear platinum(II) complexes and their emission properties.
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Utsuno M, Yutaka T, Murata M, Kurihara M, Tamai N, and Nishihara H
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- Molecular Structure, Platinum Compounds chemistry, Spectrophotometry, Time Factors, Anthraquinones chemistry, Cross-Linking Reagents chemistry, Platinum Compounds chemical synthesis
- Abstract
Anthraquinone-bridged mononuclear and dinuclear complexes, [PtCl(AQ-amide-tpy)](PF6) (1), [Pt2Cl2(AQ-amide-tpy2)](PF6)2 (2), and [Pt2Cl2(AQ-eth-tpy2)](PF6)2 (3), were synthesized and their photochemical properties were investigated. Amide-bound mononuclear complex 1 exhibited only metal-to-ligand charge transfer (MLCT) absorption and emission, whereas dinuclear complex 2 exhibited a low-energy emission around 700 nm at room temperature. Emission lifetime analysis indicated that this emission was originated from the metal-metal-to-ligand charge transfer (MMLCT) excited state, implying the existence of an intramolecular Pt-Pt interaction at the photoexcited state. 3 with rigid ethynylene linkers showed a low-energy absorption around 520 nm (epsilon = approximately 1100 M(-1) cm(-1)) in addition to an 1MLCT absorption, which was ascribed to a 3MLCT absorption from the consideration of the Pt-Pt distance on a geometry-optimized structure. The emission of 3 appeared at 600 nm, which is higher in energy compared with the emission of 2. It is postulated that the restriction of the Pt-Pt distance flexibility in the rigid structure of 3 prevents the significant increase of the Pt-Pt interaction at the excited state.
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- 2007
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35. Enhancement of antitumor activity of 5'-deoxy-5-fluorouridine (Furtulon) by taxane in human gastric cancer xenografts.
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Sawada N, Nose T, Ishikawa T, and Yutaka T
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- Animals, Bridged-Ring Compounds administration & dosage, Cell Line, Tumor, Floxuridine administration & dosage, Humans, Immunohistochemistry, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Stomach Neoplasms enzymology, Stomach Neoplasms pathology, Taxoids administration & dosage, Thymidine Phosphorylase metabolism, Time Factors, Treatment Outcome, Up-Regulation drug effects, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Stomach Neoplasms drug therapy, Xenograft Model Antitumor Assays methods
- Abstract
5'-deoxy-5-fluorouridine (5'-DFUR, Furtulon) is activated to 5-fluorouracil (5-FU) by thymidine phosphorylase (dThdPase) highly expressed in many types of tumors. In previous studies, we demonstrated that taxanes (paclitaxel or docetaxel) up-regulated the tumor levels of dThdPase and enhanced the efficacy of 5'-DFUR in human colon and mammary xenograft models. In the present study, combination therapy of 5'-DFUR with taxanes in human gastric cancer xenograft models also showed, at the least, additive anti-tumor activity without significant augmentation of toxicity. Furthermore, paclitaxel up-regulated dThdPase expression in the tumor tissues as confirmed with ELISA and immunohistochemistry. These results suggest taxanes would potentiate the efficacy of 5'-DFUR by up-regulating the tumor levels of dThdPase in gastric xenograft models. Clinical trials of 5'-DFUR in combination with taxane against gastric cancer are warranted.
- Published
- 2005
36. Syntheses and properties of emissive iridium(III) complexes with tridentate benzimidazole derivatives.
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Yutaka T, Obara S, Ogawa S, Nozaki K, Ikeda N, Ohno T, Ishii Y, Sakai K, and Haga MA
- Abstract
A series of novel emissive Ir(III) complexes having the coordination environments of [Ir(N--N--N)2]3+, [Ir(N--N--N)(N--N)Cl]2+, and [Ir(N--N--N)(N--C--N)]2+ with 2,6-bis(1-methyl-benzimidazol-2-yl)pyridine (L1, N--N--N), 1,3-bis(1-methyl-benzimidazol-2-yl)benzene (L2H, N--C--N), 4'-(4-methylphenyl)-2,2':6',2' '-terpyridine (ttpy, N--N--N), and 2,2'-bipyridine (bpy, N--N) have been synthesized and their photophysical and electrochemical properties studied. The Ir(III) complexes exhibited phosphorescent emissions in the 500-600 nm region, with lifetimes ranging from approximately 1-10 micros at 295 K. Analysis of the 0-0 energies and the redox potentials indicated that the lowest excited state of [Ir(L1)(L2)]2+ possessed the highest contribution of 3MLCT (MLCT = metal-to-ligand charge transfer) among the Ir(III) complexes, reflecting the sigma-donating ability of the tridentate ligand, ttpy < L1 < L2. The emission quantum yields (phi) of the Ir(III) complexes ranged from 0.037 to 0.19, and the highest phi value (0.19) was obtained for [Ir(L1)(bpy)Cl]2+. Radiative rate constants (k(r)) were 1.2 x 10(4) s(-1) for [Ir(ttpy)2]3+, 3.7 x 10(4) s(-1) for [Ir(L1)(bpy)Cl]2+, 3.8 x 10(4) s(-1) for [Ir(ttpy)(bpy)Cl]2+, 3.9 x 10(4) s(-1) for [Ir(L1)2]3+, and 6.6 x 10(4) s(-1) for [Ir(L1)(L2)]2+. The highest radiative rate for [Ir(L1)(L2)]2+ with the highest contribution of 3MLCT could be explained in terms of the singlet-triplet mixing induced by spin-orbit coupling of 5d electrons in the MLCT electronic configurations.
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- 2005
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37. Novel Photoisomerization of Azoferrocene with a Low-Energy MLCT Band and Significant Change of the Redox Behavior between the cis- and trans-Isomers [J. Am. Chem. Soc. 2000, 122, 12373-12374].
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Kurihara M, Matsuda T, Hirooka A, Yutaka T, and Nishihara H
- Published
- 2004
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38. Photochemical behavior of azobenzene-conjugated CoII, CoIII, and FeII bis(terpyridine) complexes.
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Yutaka T, Mori I, Kurihara M, Tamai N, and Nishihara H
- Abstract
Azobenzene-conjugated mononuclear and dinuclear terpyridyl complexes of Co(II), Co(III), and Fe(II) were synthesized, and their photoisomerization behavior was investigated. Co(II) and Co(III) complexes, [tpyCo(tpy-AB)]X(n) and [(Cotpy)(2)(tpy-AB-tpy)]X(n) (tpy-AB = C(15)N(3)H(10)-C(6)H(4)-N=NC(6)H(5), tpy-AB-tpy = C(15)N(3)H(10)-C(6)H(4)-N=NC(6)H(4)-C(15)N(3)H(10), X = PF(6) or BPh(4)), exhibit trans-to-cis photoisomerization by irradiation at 366 nm, and this behavior is dependent on solvents and counterions. For the Co(II) complexes, BPh(4) salts undergo cis-to-trans isomerization in propylene carbonate by both photoirradiation with visible light (435 nm) and heat, indicating that reversible trans-cis isomerization has occurred. [Co(tpy-AB)(2)](BPh(4))(2) shows a two-step trans-to-cis isomerization process. The trans-cis isomerization behavior of Co(III) complexes was observed only in the solvents with a low donor number such as 1,2-dichloroethane. Fe(II) complexes, [tpyFe(tpy-AB)]X(n) (X = PF(6) or BPh(4)), exhibit slight trans-to-cis photoisomerization due to the energy transfer from the azobenzene moiety to Fe(tpy)(2) moieties.
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- 2003
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39. Photoluminescence switching of azobenzene-conjugated Pt(II) terpyridine complexes by trans-cis photoisomerization.
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Yutaka T, Mori I, Kurihara M, Mizutani J, Tamai N, Kawai T, Irie M, and Nishihara H
- Abstract
Pt(II) complexes with a terpyridylazobenzene ligand (tpyAB) were newly synthesized, and their photoluminescence properties by trans-cis isomerization of the azo moiety were investigated. In these complexes, upon excitation with 366-nm light in polar solvents such as DMF, DMSO, and propylene carbonate, trans-to-cis isomerization with significant UV-vis spectral changes occurred almost completely. Cis-to-trans isomerization was observed both by irradiation with visible light and by heat. The reduction peaks due to the terpyridine and the azo group in the cyclic voltammograms of the Pt complexes were shifted in the positive direction by trans-to-cis isomerization. Emission spectral changes due to trans-cis isomerization were observed for both the tpyAB and the Pt complexes. The significant differences in the emission properties of the complex compared to tpyAB include the observation that both the excitation and emission wavelengths were shifted to lower energy, located in the visible region. Moreover, the change in emission intensity between the trans and cis forms was more significant upon excitation with UV light, because the trans form of the complexes showed absolutely no emission. Accordingly, the azobenzene-conjugated Pt(II) terpyridine complexes promise to be doubly photofunctional materials, showing complete off-on switching of emission linked to the trans-cis conformation change.
- Published
- 2002
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40. Synthesis, characterization, and photochemical properties of azobenzene-conjugated Ru(II) and Rh(III) bis(terpyridine) complexes.
- Author
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Yutaka T, Mori I, Kurihara M, Mizutani J, Kubo K, Furusho S, Matsumura K, Tamai N, and Nishihara H
- Abstract
We synthesized azobenzene-conjugated bis(terpyridine) Ru(II) and Rh(III) mononuclear and dinuclear complexes and investigated their photochemical properties on excitation of the azo pi-pi band upon 366 nm light irradiation. The Ru mononuclear complex underwent trans-to-cis photoisomerization to reach the photostationary state with only 20% of the cis form, while the Ru dinuclear complex did not isomerize at all photochemically. On the other hand, the mononuclear and dinuclear Rh complexes showed almost complete trans-to-cis photoisomerization behavior. Cis forms of the Rh complexes thermally returned to the trans form at a much slower rate than those of organic azobenzenes, but they did not isomerize photochemically. The reduction potential of the cis forms was 80 mV more negative than that of the trans forms. The photoisomerization quantum yields of the Rh complexes were strongly dependent on the polarity, viscosity, and donor site of the solvents as well as the size of the counterions. We investigated the photoisomerization process of these complexes using femtosecond absorption spectroscopy. For the Rh complexes, we observed S(n) <-- S(2) and S(n) <-- S(1) absorption bands similar to those of organic azobenzenes. For the Ru complexes, we observed very fast bleaching of the MLCT band of the Ru complex, which indicated that the energy transfer pathway to the MLCT was the primary cause of the depressed photoisomerization. The electronic structures, which were estimated from ZINDO molecular orbital calculation, supported the different photochemical reaction behavior between the Ru and Rh complexes.
- Published
- 2001
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41. Novel photoisomerization behavior of Rh binuclear complexes involving an azobenzene-bridged bis(terpyridine) ligand. Strong effects of counterion and solvent and the induction of redox potential shift.
- Author
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Yutaka T, Kurihara M, Kubo K, and Nishihara H
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- 2000
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42. Antiaggregatory, antithrombotic effects of MS-180, a novel platelet glycoprotein IIb/IIIa receptor antagonist.
- Author
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Banno H, Kawazura H, Yutaka T, Sakuma N, Kitamori T, Hosoya J, Kibayashi K, Yamashita H, Umemura K, and Nakashima M
- Subjects
- Adenosine Diphosphate pharmacology, Anesthesia, Animals, Aspirin pharmacology, Benzopyrans, Bleeding Time, Blood Platelets metabolism, Collagen pharmacology, Dogs, Dose-Response Relationship, Drug, Guinea Pigs, Humans, In Vitro Techniques, Platelet Aggregation drug effects, Acetates pharmacology, Fibrinolytic Agents pharmacology, Glycoproteins antagonists & inhibitors, Morpholines pharmacology, Platelet Aggregation Inhibitors pharmacology, Protein Binding drug effects
- Abstract
The antiaggregatory and antithrombotic effects of (S)-(-)-ethyl[6-[4-(morpholinoformimidoyl)benzamido]-3,4-dihydro-2 H-1-benzo-pyran-3-yl]acetate hydrochloride (MS-180), a novel platelet glycoprotein IIb/IIIa receptor antagonist, were investigated. Ma-HCl, (S)-(-)-[6-[4-(Morpholinoformimidoyl)benzamido]-3,4-dihydro-2H-1-b enzopyran-3-yl]acetic acid hydrochloride, the hydrochloride salt of Ma (active metabolite), inhibited the binding of fibrinogen to immobilized human glycoprotein IIb/III receptor with an IC50 value of 0.12+/-0.03 nM without affecting binding to either fibronectin or vitronectin receptors. In anesthetized guinea pigs, intraduodenal administration of MS-180 caused dose-dependent inhibition of both ADP- and collagen-induced ex vivo platelet aggregation. At the same dosages, occluded thrombus formation and platelet release reactions were also markedly suppressed. In anesthetized dogs, the bleeding time was prolonged slightly even when submaximal inhibition (< 90%) of ex vivo platelet aggregation was achieved following i.v. administration of Ma-HCl. Aspirin (100 mg/kg) prolonged the bleeding time to the same extent as MS-180 (1 mg/kg), although it suppressed only collagen-induced platelet aggregation. Therefore, MS-180 may be clinically useful for the treatment of thrombotic diseases.
- Published
- 1999
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43. A severe infantile sialidosis (beta-galactosidase-alpha-neuraminidase deficiency) mimicking GM1-gangliosidosis type 1.
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Okada S, Sugino H, Kato T, Yutaka T, Koike M, Dezawa T, Yamano T, and Yabuuchi H
- Subjects
- Diagnosis, Differential, Female, Fibroblasts enzymology, Humans, Hydrogen-Ion Concentration, Infant, Lymphocytes enzymology, Phenotype, Gangliosidoses diagnosis, Lactose Intolerance diagnosis, Neuraminidase deficiency
- Abstract
We observed a 3-month-old Japanese female infant with severe psychomotor retardation, coarse facial appearance, hepatosplenomegaly, and dysostosis multiplex. Only beta-galactosidase was found to be deficient when the routine lysosomal hydrolase assay was performed on the patient's lymphocytes at 6 months of age. At first GM1-gangliosidosis type 1 seemed the most likely diagnosis. Later, however, additional studies (hydrolase assay in cultured skin fibroblasts, urinary oligosaccharide analysis, genetic complementation study, etc.) revealed that biochemical data of this case were in agreement with those of severe infantile sialidosis. The only important exception was that alpha-neuraminidase in the patient's lymphocytes showed normal activity but abnormal pH dependence toward 4-methylumbellyferyl substrate. In addition, a severely damaged kidney suggested that his case may be classified as a unique type of severe infantile sialidosis (possible nephrosialidosis). These observations stress the importance of careful biochemical diagnosis of a case with GM1-gangliosidosis type 1 phenotype.
- Published
- 1983
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44. Neuronal depletion of cerebellum in late infantile metachromatic leukodystrophy.
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Yamano T, Ohta S, Shimada M, Okada S, Yutaka T, Sugita T, and Yabuuchi H
- Subjects
- Cerebellar Diseases pathology, Child, Humans, Leukodystrophy, Metachromatic complications, Male, Neurons ultrastructure, Purkinje Cells ultrastructure, Cerebellum pathology, Leukodystrophy, Metachromatic pathology
- Abstract
The cerebellums of two children with late infantile metachromatic leukodystrophy were investigated by light and electron microscopy. These observations showed that the morphological abnormalities were comprised not only of severe demyelination and presence of metachromatic granules but also marked loss of Purkinje cells and granule cells. The depletion of Purkinje cells is considered to have resulted from demyelination of their myelinated axons. On the other hand, the reduction of granule cells is considered to be caused by the loss of Purkinje cells and presynaptic elements such as mossy fibers and glomerular collaterals of climbing fibers. The depletion of these neurons induced destruction of synaptic connection in the cerebellum.
- Published
- 1980
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45. Electron microscopic examination of skin and conjunctival biopsy specimens in neuronal storage diseases.
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Yamano T, Shimada M, Okada S, Yutaka T, Yabuuchi H, and Nakao Y
- Subjects
- Adolescent, Axons ultrastructure, Biopsy, Child, Child, Preschool, Humans, Inclusion Bodies ultrastructure, Infant, Microscopy, Electron, Mucolipidoses pathology, Mucopolysaccharidoses pathology, Neurons ultrastructure, Niemann-Pick Diseases pathology, Tay-Sachs Disease pathology, Conjunctiva innervation, Metabolism, Inborn Errors pathology, Skin innervation
- Abstract
Skin and conjunctival biopsy specimens from fourteen patients with neuronal storage diseases were investigated using an electron microscope. The diseases were Tay-Sachs disease, ceroid-lipofuscinosis (Jansky-Bielschowsky type), Niemann-Pick disease (type B), highly suspected adrenoleukodystrophy, I-cell disease, mucolipidosis of the beta-galactosidase deficient type, Hurler disease, Hunter disease and Morquio disease. This examination provided valuable diagnostic information on some neuronal storage diseases but not on Morquio disease or highly suspected adrenoleukodystrophy. False negative results may sometimes occur using this examination method. However, this examination suggests the usefulness of skin and conjunctival biopsy specimens as a diagnostic tool in some neuronal storage diseases.
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- 1979
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46. Purification and some properties of human liver iduronate sulfatase.
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Yutaka T, Fluharty AL, Stevens RL, and Kihara H
- Subjects
- Chemical Phenomena, Chemistry, Chromatography methods, Electrophoresis, Polyacrylamide Gel, Humans, Isoelectric Focusing, Solubility, Iduronate Sulfatase isolation & purification, Liver enzymology, Sulfatases isolation & purification
- Abstract
Iduronate sulfatase was purified from human liver for an investigation of the degradative pathway of dermatan sulfate. An overall 80-fold purification was achieved and, more importantly, the preparation was free of alpha-L-iduronidase, beta-glucuronidase, N-acetylgalactosamine 4-sulfate sulfatase (arylsulfatase B) and highly enriched in beta-N-acetylhexosaminidase. The liver enzyme appeared to be composed of several molecular species. The enzyme activity was optimal at pH 4.0 and its Km was 10--20 microM with sulfoiduronyl sulfoanhydromannitol. Chloride was inhibitory at high concentration and among divalent metal ions, only copper was inhibitory. Nitrocatechol sulfate was not a substrate, but did show competitive inhibition. Its Ki for iduronate sulfatase was similar to its Km for arylsulfatase, suggesting a similarity in the substrate binding sites of iduronate sulfatase and arylsulfatases.
- Published
- 1982
- Full Text
- View/download PDF
47. Heterogeneity in mucolipidosis II (I-cell disease).
- Author
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Okada S, Kato T, Oshima T, Yutaka T, and Yabuuchi H
- Subjects
- Ammonium Chloride pharmacology, Cells, Cultured, Hexosaminidases genetics, Hexosaminidases metabolism, Humans, Isoenzymes metabolism, Lysosomes enzymology, Mannosidases metabolism, Mucolipidoses enzymology, Sucrose metabolism, Mucolipidoses classification
- Abstract
Normalization of multiple deficiency of intracellular lysosomal hydrolases in I-cell disease (ICD) fibroblasts by sucrose loading has been reported (Kato et al. (1982) J. Biol. Chem. 257, 7814). Further studies revealed that the effects of sucrose on the induction of hydrolases in seven ICD strains examined in this study were characteristic in each strain. The results may indicate that ICD strains can be classified into subgroups by the degree of enzymic induction. Moreover, this speculation seems to be supported by the normalization of electrophoretic patterns of beta-hexosaminidase in ICD fibroblasts after sucrose loading.
- Published
- 1983
- Full Text
- View/download PDF
48. A case of neuraminidase deficiency associated with a partial beta-galactosidase defect. Clinical, biochemical and radiological studies.
- Author
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Okada S, Yutaka T, Kato T, Ikehara C, Yabuuchi H, Okawa M, Inui M, and Chiyo H
- Subjects
- Cells, Cultured enzymology, Child, Female, Fibroblasts enzymology, Humans, Leukocytes enzymology, Mucolipidoses complications, Lactose Intolerance complications, Mucolipidoses diagnosis, Neuraminidase deficiency
- Abstract
Neuraminidase deficiency towards fetuin, 2 leads to 3 sialyllactose and 2 leads to 6 sialyllactose was found in cultured skin fibroblasts from a 10-year-old Japanese girl who exhibits craniofacial dysmorphism, a short neck, vertebral and pelvic deformities and macular cherry-red spots. Neuraminidase deficiency in this case seems the primary enzyme defect because the enzyme activity of her parents was intermediate. In addition, beta-galactosidase in leukocytes and cultured skin fibroblasts from the patient was found to be severely deficient, but could be detected in serum and urine. In the parents, beta-galactosidase activity was normal. There were moderately increased levels of urinary sialic acid-rich oligosaccharides and glycopeptides in the patient. The clinical and biochemical observations suggest that this case is very close to mucolipidosis I.
- Published
- 1979
- Full Text
- View/download PDF
49. Ultrastructural study on nervous system of fetus with GM1-gangliosidosis type 1.
- Author
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Yamano T, Shimada M, Okada S, Yutaka T, Kato T, Inui K, Yabuuchi H, Kanzaki S, and Kanda S
- Subjects
- Cerebellum ultrastructure, Cerebral Cortex ultrastructure, Fetus pathology, G(M1) Ganglioside, Humans, Microscopy, Electron, Myenteric Plexus ultrastructure, Parietal Lobe ultrastructure, Radial Nerve ultrastructure, Spinal Cord ultrastructure, Vacuoles ultrastructure, Gangliosidoses pathology, Nervous System ultrastructure
- Abstract
The nervous system of a 22-year-old fetus with GM1-gangliosidosis type 1 was studied by electron microscopy. The tissues thus examined were the cerebral cortex at the parietal region, the cerebellum, the thoracic spinal cord, the Auerbach's myenteric plexus in the large intestine and the radial nerve fibers. In the cerebral cortex, membrane-bound vacuoles, which occasionally contained stacks of fine fibrils, were observed in the large young neurons in the deeper part of the cortical plate. The neurons in the other part of the cerebral cortex carried no storage materials. In the cerebellum, the membrane-bound vacuoles with stacks of fine fibrils were seen only in the Purkinje cells. The neurons in the spinal cord also contained several zebra-like bodies and the above membrane-bound vacuoles. As for the peripheral nervous system (PNS), neurons in the Auerbach's myenteric plexus carried membranous cytoplasmic bodies and zebra-like bodies. Some of the axons in the radial nerve fibers also contained a lot of pleomorphic electron-dense bodies and a few membranous cytoplasmic ones. These results show that the accumulation of storage materials is started in the large neurons which are produced in the early stage of neurogenesis in the central nervous system (CNS). Additionally, the observed membrane-bound vacuoles are considered to be structures which occur before the membranous cytoplasmic bodies and/or the zebra-like bodies. It is also elucidated that the PNS is affected earlier than the cerebral and cerebellar cortices and thoracic spinal cord.
- Published
- 1983
- Full Text
- View/download PDF
50. Application of epithelial cells in culture to the biochemical studies of lysosomal hydrolase deficiencies.
- Author
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Okada S, Kato T, Yutaka T, Yamano T, and Yabuuchi H
- Subjects
- Cells, Cultured, Epithelium enzymology, Female, Humans, Male, Trypsin, Hydrolases deficiency, Lysosomes enzymology
- Abstract
Epithelial cells are readily obtained when the primary culture of skin fibroblasts is established. The major lysosomal hydrolase activities in normal epithelial cells are lower than those in normal fibroblasts. The primary deficiency of lysosomal enzymes can be detected both in epithelial cells and in cultured skin fibroblasts. However, epithelial cells of I-cell disease show only beta-galactosidase deficiency. This result indicates that epithelial cells have different biochemical properties from skin fibroblasts and they may be similar to those of visceral organs. Therefore, cultured epithelial cells seem to be useful for the studies on lysosomal hydrolase deficiencies.
- Published
- 1983
- Full Text
- View/download PDF
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