384 results on '"Svicher, V."'
Search Results
2. Unexpected rise in the circulation of complex HBV variants enriched of HBsAg vaccine-escape mutations in HBV genotype-D: potential impact on HBsAg detection/quantification and vaccination strategies
- Author
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Piermatteo, L, D'Anna, S, Bertoli, A, Bellocchi, M, Carioti, L, Fabeni, L, Alkhatib, M, Frazia, Sl, Lichtner, M, Mastroianni, C, Sanctis, Gd, Marignani, M, Pasquazzi, C, Iapadre, N, Parruti, G, Cappiello, G, Vecchiet, J, Malagnino, V, Grelli, S, Ceccherini-Silbertein, F, Andreoni, M, Sarmati, L, Svicher, V, and Salpini, R
- Subjects
HBsAg ,vaccine-escape mutations ,HBsAg secretion ,HBV ,HBsAg antigenicity ,Settore MED/07 - Published
- 2023
3. Recommendations for screening, monitoring, prevention, prophylaxis and therapy of hepatitis B virus reactivation in patients with haematologic malignancies and patients who underwent haematologic stem cell transplantation—a position paper
- Author
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Sarmati, L., Andreoni, M., Antonelli, G., Arcese, W., Bruno, R., Coppola, N., Gaeta, G.B., Galli, M., Girmenia, C., Mikulska, M., Pane, F., Perno, C.F., Picardi, M., Puoti, M., Rambaldi, A., Svicher, V., Taliani, G., and Gentile, G.
- Published
- 2017
- Full Text
- View/download PDF
4. HDV epidemic in Central Italy is stable over the last two decades and is characterized by the circulation of multiple HDV sub-genotypes 1 inducing different inflammatory stimuli
- Author
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Salpini, R., primary, Piermatteo, L., additional, Torre, G., additional, D'Anna, S., additional, Khan, S., additional, Duca, L., additional, Bertoli, A., additional, Malagnino, V., additional, Paba, P., additional, Ciotti, M., additional, Lenci, I., additional, Francioso, S., additional, Paquazzi, C., additional, Lichtner, M., additional, Mastroianni, C., additional, Santopaolo, F., additional, De Sanctis, G., additional, Marignani, M., additional, Pellicelli, A., additional, Galati, G., additional, Moretti, A., additional, Colucci, G., additional, Casinelli, K., additional, Caterini, L., additional, Iapadre, N., additional, Parruti, G., additional, Vecchiet, I., additional, Paoloni, M., additional, Grelli, S., additional, Ceccherini-Silberstein, F., additional, Sarmati, L., additional, and Svicher, V., additional
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- 2023
- Full Text
- View/download PDF
5. The novel HBx mutation F30V correlates with hepatocellular carcinoma in vivo, reduces hepatitis B virus replicative efficiency and enhances anti-apoptotic activity of HBx N terminus in vitro
- Author
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Salpini, R., Surdo, M., Cortese, M.F., Palumbo, G.A., Carioti, L., Cappiello, G., Spanò, A., Trimoulet, P., Fleury, H., Vecchiet, J., Pasquazzi, C., Mirabelli, C., Scutari, R., Sacco, A., Alkhatib, M., Missale, G., Francioso, S., Sarmati, L., Andreoni, M., Angelico, M., Ceccherini-Silberstein, F., Levrero, M., Perno, C.F., Belloni, L., and Svicher, V.
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- 2019
- Full Text
- View/download PDF
6. The impact of DAA-mediated HCV eradication on CD4+ and CD8+ T lymphocyte trajectories in HIV/HCV coinfected patients: Data from the ICONA Foundation Cohort
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Bandera, A., Lorenzini, P., Taramasso, L., Cozzi-Lepri, A., Lapadula, G., Mussini, C., Saracino, A., Ceccherini-Silberstein, F., Puoti, M., Quiros-Roldan, E., Montagnani, F., Antinori, A., d'Arminio Monforte, A., Gori, A., Andreoni, M., Castagna, A., Castelli, F., Cauda, R., Di Perri, G., Galli, M., Iardino, R., Ippolito, G., Lazzarin, A., Marchetti, G. C., Rezza, G., von Shloesser, F., Viale, P., Girardi, E., Lo Caputo, S., Maggiolo, F., Perno, C. F., Bai, F., Bonora, S., Borderi, M., Calcagno, A., Capobianchi, M. R., Cicalini, S., Cingolani, A., Cinque, P., Di Biagio, A., Gagliardini, R., Gianotti, N., Guaraldi, G., Lichtner, M., Lai, A., Madeddu, G., Marchetti, G., Merlini, E., Nozza, S., Piconi, S., Pinnetti, C., Rossotti, R., Rusconi, S., Santoro, M. M., Sarmati, L., Spagnuolo, V., Svicher, V., Fanti, I., Galli, L., Rodano', A., Macchia, M., Tavelli, A., Bove, A., Camposeragna, A., Errico, M., Manfredini, M., Perziano, A., Calvino, V., Carletti, F., Carrara, S., Di Caro, A., Graziano, S., Petroni, F., Prota, G., Truffa, S., Giacometti, A., Costantini, A., Barocci, V., Angarano, G., Monno, L., Milano, E., Suardi, C., Donati, V., Verucchi, G., Castelnuovo, F., Minardi, C., Menzaghi, B., Abeli, C., Chessa, L., Pes, F., Cacopardo, B., Celesia, B., Vecchiet, J., Falasca, K., Pan, A., Lorenzotti, S., Sighinolfi, L., Segala, D., Blanc, P., Vichi, F., Cassola, G., Bassetti, M., Alessandrini, A., Bobbio, N., Mazzarello, G., Fondaco, L., Bonfanti, P., Molteni, C., Chiodera, A., Milini, P., Nunnari, G., Pellicano, G., Rizzardini, G., Cannizzo, E. S., Moioli, M. C., Piolini, R., Bernacchia, D., Poli, A., Tincati, C., Puzzolante, C., Migliorino, C., Sangiovanni, V., Borgia, G., Esposito, V., Di Flumeri, G., Gentile, I., Rizzo, V., Cattelan, A. M., Marinello, S., Cascio, A., Trizzino, M., Francisci, D., Schiaroli, E., Parruti, G., Sozio, F., Lazzaretti, C., Corsini, R., Cristaudo, A., Vullo, V., Acinapura, R., Lamonica, S., Capozzi, M., Mondi, A., Rivano Capparuccia, M., Iaiani, G., Latini, A., Onnelli, G., Plazzi, M. M., De Girolamo, G., Vergori, A., Cecchetto, M., Viviani, F., De Vito, A., Rossetti, B., Franco, A., Fontana Del Vecchio, R., Di Giuli, C., Caramello, P., Orofino, G. C., Sciandra, M., Londero, A., Manfrin, V., Battagin, G., Starnini, G., Ialungo, A., Bandera A., Lorenzini P., Taramasso L., Cozzi-Lepri A., Lapadula G., Mussini C., Saracino A., Ceccherini-Silberstein F., Puoti M., Quiros-Roldan E., Montagnani F., Antinori A., d'Arminio Monforte A., Gori A., Andreoni M., Castagna A., Castelli F., Cauda R., Di Perri G., Galli M., Iardino R., Ippolito G., Lazzarin A., Marchetti G.C., Rezza G., von Shloesser F., Viale P., Girardi E., Lo Caputo S., Maggiolo F., Perno C.F., Bai F., Bonora S., Borderi M., Calcagno A., Capobianchi M.R., Cicalini S., Cingolani A., Cinque P., Di Biagio A., Gagliardini R., Gianotti N., Guaraldi G., Lichtner M., Lai A., Madeddu G., Merlini E., Nozza S., Piconi S., Pinnetti C., Rossotti R., Rusconi S., Santoro M.M., Sarmati L., Spagnuolo V., Svicher V., Fanti I., Galli L., Rodano' A., Macchia M., Tavelli A., Bove A., Camposeragna A., Errico M., Manfredini M., Perziano A., Calvino V., Carletti F., Carrara S., Di Caro A., Graziano S., Petroni F., Prota G., Truffa S., Giacometti A., Costantini A., Barocci V., Angarano G., Monno L., Milano E., Suardi C., Donati V., Verucchi G., Castelnuovo F., Minardi C., Menzaghi B., Abeli C., Chessa L., Pes F., Cacopardo B., Celesia B., Vecchiet J., Falasca K., Pan A., Lorenzotti S., Sighinolfi L., Segala D., Blanc P., Vichi F., Cassola G., Bassetti M., Alessandrini A., Bobbio N., Mazzarello G., Fondaco L., Bonfanti P., Molteni C., Chiodera A., Milini P., Nunnari G., Pellicano G., Rizzardini G., Cannizzo E.S., Moioli M.C., Piolini R., Bernacchia D., Poli A., Tincati C., Puzzolante C., Migliorino C., Sangiovanni V., Borgia G., Esposito V., Di Flumeri G., Gentile I., Rizzo V., Cattelan A.M., Marinello S., Cascio A., Trizzino M., Francisci D., Schiaroli E., Parruti G., Sozio F., Lazzaretti C., Corsini R., Qqwdreoni Q., Cristaudo A., Vullo V., Acinapura R., Lamonica S., Capozzi M., Mondi A., Rivano Capparuccia M., Iaiani G., Latini A., Onnelli G., Plazzi M.M., De Girolamo G., Vergori A., Cecchetto M., Viviani F., De Vito A., Rossetti B., Franco A., Fontana Del Vecchio R., Di Giuli C., Caramello P., Orofino G.C., Sciandra M., Londero A., Manfrin V., Battagin G., Starnini G., Ialungo A., Bandera, A, Lorenzini, P, Taramasso, L, Cozzi-Lepri, A, Lapadula, G, Mussini, C, Saracino, A, Ceccherini-Silberstein, F, Puoti, M, Quiros-Roldan, E, Montagnani, F, Antinori, A, d'Arminio Monforte, A, Gori, A, Andreoni, M, Castagna, A, Castelli, F, Cauda, R, Di Perri, G, Galli, M, Iardino, R, Ippolito, G, Lazzarin, A, Marchetti, G, Rezza, G, von Shloesser, F, Viale, P, Girardi, E, Lo Caputo, S, Maggiolo, F, Perno, C, Bai, F, Bonora, S, Borderi, M, Calcagno, A, Capobianchi, M, Cicalini, S, Cingolani, A, Cinque, P, Di Biagio, A, Gagliardini, R, Gianotti, N, Guaraldi, G, Lichtner, M, Lai, A, Madeddu, G, Merlini, E, Nozza, S, Piconi, S, Pinnetti, C, Rossotti, R, Rusconi, S, Santoro, M, Sarmati, L, Spagnuolo, V, Svicher, V, Fanti, I, Galli, L, Rodano', A, Macchia, M, Tavelli, A, Bove, A, Camposeragna, A, Errico, M, Manfredini, M, Perziano, A, Calvino, V, Carletti, F, Carrara, S, Di Caro, A, Graziano, S, Petroni, F, Prota, G, Truffa, S, Giacometti, A, Costantini, A, Barocci, V, Angarano, G, Monno, L, Milano, E, Suardi, C, Donati, V, Verucchi, G, Castelnuovo, F, Minardi, C, Menzaghi, B, Abeli, C, Chessa, L, Pes, F, Cacopardo, B, Celesia, B, Vecchiet, J, Falasca, K, Pan, A, Lorenzotti, S, Sighinolfi, L, Segala, D, Blanc, P, Vichi, F, Cassola, G, Bassetti, M, Alessandrini, A, Bobbio, N, Mazzarello, G, Fondaco, L, Bonfanti, P, Molteni, C, Chiodera, A, Milini, P, Nunnari, G, Pellicano, G, Rizzardini, G, Cannizzo, E, Moioli, M, Piolini, R, Bernacchia, D, Poli, A, Tincati, C, Puzzolante, C, Migliorino, C, Sangiovanni, V, Borgia, G, Esposito, V, Di Flumeri, G, Gentile, I, Rizzo, V, Cattelan, A, Marinello, S, Cascio, A, Trizzino, M, Francisci, D, Schiaroli, E, Parruti, G, Sozio, F, Lazzaretti, C, Corsini, R, Qqwdreoni, Q, Cristaudo, A, Vullo, V, Acinapura, R, Lamonica, S, Capozzi, M, Mondi, A, Rivano Capparuccia, M, Iaiani, G, Latini, A, Onnelli, G, Plazzi, M, De Girolamo, G, Vergori, A, Cecchetto, M, Viviani, F, De Vito, A, Rossetti, B, Franco, A, Fontana Del Vecchio, R, Di Giuli, C, Caramello, P, Orofino, G, Sciandra, M, Londero, A, Manfrin, V, Battagin, G, Starnini, G, Ialungo, A, Bandera, A., Lorenzini, P., Taramasso, L., Cozzi-Lepri, A., Lapadula, G., Mussini, C., Saracino, A., Ceccherini-Silberstein, F., Puoti, M., Quiros-Roldan, E., Montagnani, F., Antinori, A., d'Arminio Monforte, A., Gori, A., Andreoni, M., Castagna, A., Castelli, F., Cauda, R., Di Perri, G., Galli, M., Iardino, R., Ippolito, G., Lazzarin, A., Marchetti, G. C., Rezza, G., von Shloesser, F., Viale, P., Girardi, E., Lo Caputo, S., Maggiolo, F., Perno, C. F., Bai, F., Bonora, S., Borderi, M., Calcagno, A., Capobianchi, M. R., Cicalini, S., Cingolani, A., Cinque, P., Di Biagio, A., Gagliardini, R., Gianotti, N., Guaraldi, G., Lichtner, M., Lai, A., Madeddu, G., Merlini, E., Nozza, S., Piconi, S., Pinnetti, C., Rossotti, R., Rusconi, S., Santoro, M. M., Sarmati, L., Spagnuolo, V., Svicher, V., Fanti, I., Galli, L., Rodano', A., Macchia, M., Tavelli, A., Bove, A., Camposeragna, A., Errico, M., Manfredini, M., Perziano, A., Calvino, V., Carletti, F., Carrara, S., Di Caro, A., Graziano, S., Petroni, F., Prota, G., Truffa, S., Giacometti, A., Costantini, A., Barocci, V., Angarano, G., Monno, L., Milano, E., Suardi, C., Donati, V., Verucchi, G., Castelnuovo, F., Minardi, C., Menzaghi, B., Abeli, C., Chessa, L., Pes, F., Cacopardo, B., Celesia, B., Vecchiet, J., Falasca, K., Pan, A., Lorenzotti, S., Sighinolfi, L., Segala, D., Blanc, P., Vichi, F., Cassola, G., Bassetti, M., Alessandrini, A., Bobbio, N., Mazzarello, G., Fondaco, L., Bonfanti, P., Molteni, C., Chiodera, A., Milini, P., Nunnari, G., Pellicano, G., Rizzardini, G., Cannizzo, E. S., Moioli, M. C., Piolini, R., Bernacchia, D., Poli, A., Tincati, C., Puzzolante, C., Migliorino, C., Sangiovanni, V., Borgia, G., Esposito, V., Di Flumeri, G., Gentile, I., Rizzo, V., Cattelan, A. M., Marinello, S., Cascio, A., Trizzino, M., Francisci, D., Schiaroli, E., Parruti, G., Sozio, F., Lazzaretti, C., Corsini, R., Qqwdreoni, Q., Cristaudo, A., Vullo, V., Acinapura, R., Lamonica, S., Capozzi, M., Mondi, A., Rivano Capparuccia, M., Iaiani, G., Latini, A., Onnelli, G., Plazzi, M. M., De Girolamo, G., Vergori, A., Cecchetto, M., Viviani, F., De Vito, A., Rossetti, B., Franco, A., Fontana Del Vecchio, R., Di Giuli, C., Caramello, P., Orofino, G. C., Sciandra, M., Londero, A., Manfrin, V., Battagin, G., Starnini, G., and Ialungo, A.
- Subjects
CD4-Positive T-Lymphocytes ,HIV Infections ,Hepacivirus ,CD8-Positive T-Lymphocytes ,Gastroenterology ,Settore MED/07 ,chemistry.chemical_compound ,0302 clinical medicine ,Cd8 t lymphocyte ,HIV Infection ,030212 general & internal medicine ,Coinfection ,CD4 ,CD8 ,DAA ,HCV/HIV ,immune activation ,Hcv clearance ,virus diseases ,Middle Aged ,Hepatitis C ,Infectious Diseases ,medicine.anatomical_structure ,CD4-Positive T-Lymphocyte ,Cohort ,030211 gastroenterology & hepatology ,Female ,CD4, CD8, DAA, HCV/HIV, immune activation ,Human ,Immune activation ,medicine.medical_specialty ,T cell ,Antiviral Agents ,NO ,03 medical and health sciences ,Virology ,Internal medicine ,medicine ,Humans ,In patient ,Antiviral Agent ,Hepaciviru ,Hepatology ,business.industry ,Ribavirin ,CD8-Positive T-Lymphocyte ,CD4 Lymphocyte Count ,chemistry ,business - Abstract
HCV infection has been hypothesized as a contributor of poor CD4+ recovery in patients living with HIV (PLWHIV). Aim of this study was to evaluate CD4+, CD8+ cells and CD4/CD8 ratio trends before and after HCV treatment with direct acting agents (DAA) in PLWHIV. HIV/HCV patients enrolled in ICONA and HepaICONA cohorts with HIV-RNA≤50copies/ml who achieved a sustained viral response after DAA treatment were studied. A linear regression model was used to investigate CD4+, CD8+ and CD4/CD8 changes 12months before and after DAA treatment. A total of 939 HIV/HCV patients were included, 225 (24.0%) female, median age: 53years (IQR 50–56). At DAA initiation, CD4+ T cell count was 12.5kPa. Trends of CD4+ and CD4/CD8 ratio were similar before and after DAA in all study populations (CD4+ change +17.6 cells/mm3 (95%CI −33.5; 69.4, p=0.494); CD4/CD8 change 0.013 (95%CI −0.061; 0.036, p=0.611). However, patients treated with ribavirin (RBV)-free DAA showed a significant decrease in CD8+ cells (−204.3 cells/mm3, 95%CI −375.0;-33.4, p=0.019), while patients treated with RBV experienced CD8+ cell increase (+141.2 cells/mm3, 95%CI 40.3; 242.1, p=0.006). In conclusion, HCV eradication following DAA treatment does not seem to have an impact on CD4+ T cell recovery in PLWHIV. However, a fast decline of CD8+T cells has been observed in patients treated without RBV, suggesting a favourable effect of HCV clearance on the general state of immune activation.
- Published
- 2021
7. Hepatitis B virus DNA integration as a novel biomarker of hepatitis B virus-mediated pathogenetic properties and a barrier to the current strategies for hepatitis B virus cure
- Author
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Salpini, R, D'Anna, S, Benedetti, L, Piermatteo, L, Gill, U, Svicher, V, and Kennedy, Ptf
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Microbiology (medical) ,HBV cure ,chronic HBV infection ,hepatocellular carcinoma ,HBV biomarkers ,HBV-DNA integration ,Microbiology ,Settore MED/07 - Abstract
Chronic infection with Hepatitis B Virus (HBV) is a major cause of liver-related morbidity and mortality worldwide. HBV-DNA integration into the human genome is recognized as a frequent event occurring during the early phases of HBV infection and characterizing the entire course of HBV natural history. The development of refined molecular biology technologies sheds new light on the functional implications of HBV-DNA integration into the human genome, including its role in the progression of HBV-related pathogenesis and in triggering the establishment of pro-oncogenic mechanisms, promoting the development of hepatocellular carcinoma. The present review provides an updated and comprehensive overview of the current body of knowledge on HBV-DNA integration, focusing on the molecular mechanisms underlying HBV-DNA integration and its occurrence throughout the different phases characterizing the natural history of HBV infection. Furthermore, here we discuss the main clinical implications of HBV integration as a biomarker of HBV-related pathogenesis, particularly in reference to hepatocarcinogenesis, and how integration may act as a barrier to the achievement of HBV cure with current and novel antiviral therapies. Overall, a more refined insight into the mechanisms and functionality of HBV integration is paramount, since it can potentially inform the design of ad hoc diagnostic tools with the ability to reveal HBV integration events perturbating relevant intracellular pathways and for identifying novel therapeutic strategies targeting alterations directly related to HBV integration.
- Published
- 2022
8. In the setting of HIV-infection, HBV-reactivation is revealed by highly sensitive assays in a conspicuous fraction of anti-HBc-positive/HBsAg-negative patients switching to Tenofovir-sparing therapy
- Author
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Salpini, R, D’Anna, S, Alkhatib, M, Piermatteo, L, Tavelli, A, Quiros-Roldan, E, Cingolani, A, Papalini, C, Carrara, S, Puoti, M, Sarmati, L, d’Arminio Monforte, A, Ceccherini-Silberstein, A, Perno, C, and Svicher, V
- Subjects
Hepatology ,Settore MED/06 - Published
- 2022
9. Evidence of abundant transcriptionally active HBV-integration events involving genetic regions crucial for cell proliferation in the setting of HBeAg positive infection
- Author
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D’Anna, S, Salpini, R, Carioti, L, Battisti, A, Piermatteo, L, Benedetti, L, Ceccherini-Silberstein, F, Gill, Us, Svicher, V, and Kennedy, Ptf
- Subjects
Settore MED/07 - Published
- 2022
10. An in-depth characterization of VOCs circulation by using NGS analysis of the Spike protein
- Author
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Bellocchi, Mc, Carioti, L, Scutari, R, Iannetta, M, Piermatteo, L, Alkhatib, M, Tedde, S, Duca, L, Coppola, L, Malagnino, V, Crea, A, Ansaldo, L, D’Anna, S, Santoro, Mm, Bertoli, A, Di Lorenzo, A, Salpini, R, Svicher, V, Teti, E, Braccialarghe, N, Cavasio, Ra, Sarmati, L, Andreoni, M, and Ceccherini-Silberstein, F
- Subjects
Settore MED/07 - Published
- 2022
11. POTENT ANTIVIRAL ACTIVITY OF NEW GENERATION HIV-1 MATURATION INHIBITORS ON HUMAN PRIMARY CELLS
- Author
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Duca, L, Saccomandi, P, Piermatteo, L, Salpini, R, Stacchiotti, C, Aquaro, S, Perno, Cf, Svicher, V, and Ceccherini-Silberstein, F
- Subjects
Settore MED/07 - Published
- 2022
12. Transcriptionally active HBV integrations frequently occur in the early phases of chronic infection and mostly involve genetic regions crucial for cell proliferation
- Author
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D’Anna, S, Carioti, L, Piermatteo, L, Battisti, A, Benedetti, L, Ceccherini-Silberstein, F, Gill, Us, Kennedy, Ptf, Svicher, V, and Salpini, R
- Subjects
Settore MED/07 - Published
- 2022
13. The transactivation of endogenous retroviruses correlates with HIV reservoir, lymphocytes activation and low CD4 count in virologically suppressed patients
- Author
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Matteucci, C, Balestrieri, E, Scutari, R, Malagnino, V, Piermatteo, L, Petrone, V, Compagno, M, Salpini, R, Teti, E, Iannetta, M, Minutolo, A, Santoro, Mm, Svicher, V, Andreoni, M, Sarmati, L, Ceccherini-Silberstein, F, and S. Grelli.
- Subjects
Settore MED/07 - Published
- 2022
14. The acquisition of positively charged amino acids in HBsAg C- terminus impairs HBsAg secretion, affects its structural stability and is associated with HBV-induced liver cancer
- Author
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Piermatteo, L, Carioti, L, Duca, L, Saccomandi, P, Cappiello, G, Trimoulet, P, Fleury, H, Francioso, S, Lenci, I, Andreoni, M, Angelico, M, Minutolo, A, Matteucci, C, Sarmati, L, Svicher, V, and Salpini, R
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Settore MED/07 - Published
- 2022
15. Virological reactivation of hepatitis B virus is a frequent event in anti-HBc-positive/HBsAg-negative HIV-infected patients switching to Tenofovir-sparing therapy as revealed by highly-sensitive HBV assays
- Author
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Salpini, R, D’Anna, S, Alkhatib, M, Piermatteo, L, Tavelli, A, Quiros, E, Cingolani, A, Papalini, C, Carrara, S, Malagnino, V, Puoti, M, Sarmati, L, d’Arminio Monforte, A, Ceccherini Silberstein, F, and Svicher, V
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Settore MED/07 - Published
- 2022
16. HBcrAg tightly correlates with elevated HDV replicative activity and with enhanced liver inflammation and damage: role of HBcrAg as a biomarker of liver disease progression in the setting of HDV co-infection
- Author
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Salpini, R, Piermatteo, L, Gill, U, Battisti, A, Alkathib, M, D'Anna, S, Scutari, R, Andreassi, E, Ho, K, Ceccherini-Silberstein, F, Usai, C, Kennedy, P, and Svicher, V
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Settore MED/07 - Published
- 2022
17. Circulation of sars-cov-2 variants in central Italy: spike variability characterization by deep-sequencing
- Author
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Bellocchi, M, Carioti, L, Scutari, R, Iannetta, M, Piermatteo, L, Alkhatib, M, Tedde, S, Duca, L, Coppola, L, Malagnino, V, Crea, A, Ansaldo, L, D’Anna, S, Santoro, Mm, Bertoli, A, Di Lorenzo, A, Salpini, R, Svicher, V, Teti, E, Braccialarghe, N, Cavasio, Ra, Sarmati, L, Andreoni, M, and F. Ceccherini-Silberstein.
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Settore MED/07 - Published
- 2022
18. for the Icona Foundation Study Group Reactivation of hepatitis B virus is a frequent event in anti-HBc-positive/HBsAg-negative HIV-infected patients switching to Tenofovir sparing therapy as revealed by highly sensitive HBV assays
- Author
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Salpini, R, D’Anna, S, Alkhatib, M, Piermatteo, L, Tavelli, A, Quiros, E, Cingolani, A, Papalini, C, Carrara, S, Malagnino, V, Puoti, M, Sarmati, L, A D’Arminio Monforte, F Ceccherini Silberstein, and Svicher, V
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HIV ,Settore MED/07 - Published
- 2022
19. Evidence of extensive transcriptionally active HBV integrations involving genetic regions crucial for cell proliferation in the early phases of chronic infection even in the setting of limited liver damage
- Author
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D’Anna, S, Carioti, L, Piermatteo, L, Battisti, A, Benedetti, L, Ceccherini-Silberstein, F, Gill, Us, Kennedy, Ptf, Svicher, V, and Salpini, R
- Subjects
Settore MED/07 - Published
- 2022
20. The acquisition of positively charged amino acids in HBsAg C-terminus correlates with HBV-induced liver cancer in vivo, impairs HBsAg secretion and affects its structural stability in vitro
- Author
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Piermatteo, L, Carioti, L, Duca, L, Saccomandi, P, Cappiello, G, Trimoulet, P, Fleury, H, Francioso, S, Lenci, I, Andreoni, M, Angelico, M, Minutolo, A, Matteucci, C, Sarmati, L, Svicher, V, and R. Salpini.
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Settore MED/07 - Published
- 2022
21. Serum HBsAg and ddPCR HBV-DNA as predictive parameters of HBsAg loss after nucleo (s) tide analogue (NA) treatment discontinuation in non-cirrhotic patients with Chronic Hepatitis B
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Guerra, Af, Tomassoli, G, Piermatteo, L, D'Anna, S, Salpini, R, Svicher, V, and Ventura, P
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Settore MED/07 - Published
- 2022
22. Serum HBsAg and highly-sensitive HBV-DNA quantification can predict HBsAg loss after nucleos(t)ide analogue systematic discontinuation in non cirrhotic patients with chronic hepatitis B
- Author
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Piermatteo, L, Salpini, R, D’Anna, S, Guerra, Af, Tomassoli, G, Abbati, G, Pietrangelo, A, Ventura, P, and Svicher, V
- Subjects
hepatitis ,Settore MED/07 - Published
- 2022
23. Evaluation of HIV transmission clusters among natives and foreigners living in Italy
- Author
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Fabeni L., Santoro M. M., Lorenzini P., Rusconi S., Gianotti N., Costantini A., Sarmati L., Antinori A., Ceccherini-Silberstein F., d'Arminio Monforte A., Saracino A., Girardi E., Castagna A., Castelli F., Cauda R., Di Perri G., Galli M., Iardino R., Ippolito G., Lazzarin A., Marchetti G. C., Rezza G., von Schloesser F., Viale P., Cozzi-Lepri A., Gori A., Lo Caputo S., Maggiolo F., Mussini C., Puoti M., Perno C. F., Bai F., Bandera A., Bonora S., Borderi M., Calcagno A., Capobianchi M. R., Cicalini S., Cingolani A., Cinque P., Di Biagio A., Gagliardini R., Guaraldi G., Lapadula G., Lichtner M., Lai A., Madeddu G., Merlini E., Nozza S., Piconi S., Pinnetti C., Quiros Roldan E., Rossotti R., Spagnuolo V., Svicher V., Taramasso L., Fanti I., Galli L., Rodano A., Macchia M., Tavelli A., Bove A., Camposeragna A., Errico M., Manfredini M., Perziano A., Calvino V., Carrara S., Di Caro A., Graziano S., Petroni F., Prota G., Truffa S., Giacometti A., Barocci V., Angarano G., Monno L., Milano E., Suardi C., Donati V., Verucchi G., Castelnuovo F., Minardi C., Menzaghi B., Abeli C., Chessa L., Pes F., Cacopardo B., Celesia B., Vecchiet J., Falasca K., Pan A., Lorenzotti S., Sighinolfi L., Segala D., Blanc P., Vichi F., Cassola G., Bassetti M., Alessandrini A., Bobbio N., Mazzarello G., Fondaco L., Bonfanti P., Molteni C., Chiodera A., Milini P., Nunnari G., Pellicano G., Rizzardini G., Cannizzo E. S., Moioli M. C., Piolini R., Bernacchia D., Poli A., Tincati C., Puzzolante C., Migliorino C., Sangiovanni V., Borgia G., Esposito V., Di Flumeri G., Gentile I., Rizzo V., Cattelan A. M., Marinello S., Cascio A., Trizzino M., Francisci D., Schiaroli E., Parruti G., Sozio F., Lazzaretti C., Corsini R., Cristaudo A., Vullo V., Acinapura R., Lamonica S., Capozzi M., Mondi A., Rivano Capparuccia M., Iaiani G., Latini A., Onnelli G., Plazzi M. M., de Girolamo G., Vergori A., Cecchetto M., Viviani F., de Vito A., Rossetti B., Montagnani F., Franco A., Fontana Del Vecchio R., Di Giuli C., Caramello P., Orofino G. C., Sciandra M., Londero A., Manfrin V., Battagin G., Starnini G., Ialungo A., Fabeni L., Santoro M.M., Lorenzini P., Rusconi S., Gianotti N., Costantini A., Sarmati L., Antinori A., Ceccherini-Silberstein F., d'Arminio Monforte A., Saracino A., Girardi E., Castagna A., Castelli F., Cauda R., Di Perri G., Galli M., Iardino R., Ippolito G., Lazzarin A., Marchetti G.C., Rezza G., von Schloesser F., Viale P., Cozzi-Lepri A., Gori A., Lo Caputo S., Maggiolo F., Mussini C., Puoti M., Perno C.F., Bai F., Bandera A., Bonora S., Borderi M., Calcagno A., Capobianchi M.R., Cicalini S., Cingolani A., Cinque P., Di Biagio A., Gagliardini R., Guaraldi G., Lapadula G., Lichtner M., Lai A., Madeddu G., Merlini E., Nozza S., Piconi S., Pinnetti C., Quiros Roldan E., Rossotti R., Spagnuolo V., Svicher V., Taramasso L., Fanti I., Galli L., Rodano A., Macchia M., Tavelli A., Bove A., Camposeragna A., Errico M., Manfredini M., Perziano A., Calvino V., Carrara S., Di Caro A., Graziano S., Petroni F., Prota G., Truffa S., Giacometti A., Barocci V., Angarano G., Monno L., Milano E., Suardi C., Donati V., Verucchi G., Castelnuovo F., Minardi C., Menzaghi B., Abeli C., Chessa L., Pes F., Cacopardo B., Celesia B., Vecchiet J., Falasca K., Pan A., Lorenzotti S., Sighinolfi L., Segala D., Blanc P., Vichi F., Cassola G., Bassetti M., Alessandrini A., Bobbio N., Mazzarello G., Fondaco L., Bonfanti P., Molteni C., Chiodera A., Milini P., Nunnari G., Pellicano G., Rizzardini G., Cannizzo E.S., Moioli M.C., Piolini R., Bernacchia D., Poli A., Tincati C., Puzzolante C., Migliorino C., Sangiovanni V., Borgia G., Esposito V., Di Flumeri G., Gentile I., Rizzo V., Cattelan A.M., Marinello S., Cascio A., Trizzino M., Francisci D., Schiaroli E., Parruti G., Sozio F., Lazzaretti C., Corsini R., Cristaudo A., Vullo V., Acinapura R., Lamonica S., Capozzi M., Mondi A., Rivano Capparuccia M., Iaiani G., Latini A., Onnelli G., Plazzi M.M., de Girolamo G., Vergori A., Cecchetto M., Viviani F., de Vito A., Rossetti B., Montagnani F., Franco A., Fontana Del Vecchio R., Di Giuli C., Caramello P., Orofino G.C., Sciandra M., Londero A., Manfrin V., Battagin G., Starnini G., Ialungo A., Fabeni, L., Santoro, M. M., Lorenzini, P., Rusconi, S., Gianotti, N., Costantini, A., Sarmati, L., Antinori, A., Ceccherini-Silberstein, F., d'Arminio Monforte, A., Saracino, A., Girardi, E., Castagna, A., Castelli, F., Cauda, R., Di Perri, G., Galli, M., Iardino, R., Ippolito, G., Lazzarin, A., Marchetti, G. C., Rezza, G., von Schloesser, F., Viale, P., Cozzi-Lepri, A., Gori, A., Lo Caputo, S., Maggiolo, F., Mussini, C., Puoti, M., Perno, C. F., Bai, F., Bandera, A., Bonora, S., Borderi, M., Calcagno, A., Capobianchi, M. R., Cicalini, S., Cingolani, A., Cinque, P., Di Biagio, A., Gagliardini, R., Guaraldi, G., Lapadula, G., Lichtner, M., Lai, A., Madeddu, G., Merlini, E., Nozza, S., Piconi, S., Pinnetti, C., Quiros Roldan, E., Rossotti, R., Spagnuolo, V., Svicher, V., Taramasso, L., Fanti, I., Galli, L., Rodano, A., Macchia, M., Tavelli, A., Bove, A., Camposeragna, A., Errico, M., Manfredini, M., Perziano, A., Calvino, V., Carrara, S., Di Caro, A., Graziano, S., Petroni, F., Prota, G., Truffa, S., Giacometti, A., Barocci, V., Angarano, G., Monno, L., Milano, E., Suardi, C., Donati, V., Verucchi, G., Castelnuovo, F., Minardi, C., Menzaghi, B., Abeli, C., Chessa, L., Pes, F., Cacopardo, B., Celesia, B., Vecchiet, J., Falasca, K., Pan, A., Lorenzotti, S., Sighinolfi, L., Segala, D., Blanc, P., Vichi, F., Cassola, G., Bassetti, M., Alessandrini, A., Bobbio, N., Mazzarello, G., Fondaco, L., Bonfanti, P., Molteni, C., Chiodera, A., Milini, P., Nunnari, G., Pellicano, G., Rizzardini, G., Cannizzo, E. S., Moioli, M. C., Piolini, R., Bernacchia, D., Poli, A., Tincati, C., Puzzolante, C., Migliorino, C., Sangiovanni, V., Borgia, G., Esposito, V., Di Flumeri, G., Gentile, I., Rizzo, V., Cattelan, A. M., Marinello, S., Cascio, A., Trizzino, M., Francisci, D., Schiaroli, E., Parruti, G., Sozio, F., Lazzaretti, C., Corsini, R., Cristaudo, A., Vullo, V., Acinapura, R., Lamonica, S., Capozzi, M., Mondi, A., Rivano Capparuccia, M., Iaiani, G., Latini, A., Onnelli, G., Plazzi, M. M., de Girolamo, G., Vergori, A., Cecchetto, M., Viviani, F., de Vito, A., Rossetti, B., Montagnani, F., Franco, A., Fontana Del Vecchio, R., Di Giuli, C., Caramello, P., Orofino, G. C., Sciandra, M., Londero, A., Manfrin, V., Battagin, G., Starnini, G., and Ialungo, A.
- Subjects
0301 basic medicine ,Male ,lcsh:QR1-502 ,Subtype ,HIV Infections ,migrants ,Bioinformatics ,Cluster detection ,Drug resistance testing ,Human immunodeficiency virus (HIV) ,Migrants ,Molecular epidemiology ,Phylogenetic analysis ,Risk factors ,Subtypes ,Transmission networks and clusters ,Adult ,Cohort Studies ,Female ,Genotype ,HIV-1 ,Humans ,Italy ,Middle Aged ,Sexual and Gender Minorities ,pol Gene Products, Human Immunodeficiency Virus ,Emigrants and Immigrants ,Phylogeny ,lcsh:Microbiology ,Men who have sex with men ,0302 clinical medicine ,human immunodeficiency virus (HIV) ,molecular epidemiology ,phylogenetic analysis ,cluster detection ,transmission networks and clusters ,subtypes ,drug resistance testing ,risk factors ,bioinformatics ,Medicine ,030212 general & internal medicine ,pol Gene Products ,Hiv transmission ,Transmission (medicine) ,virus diseases ,Infectious Diseases ,Cohort ,Human Immunodeficiency Virus ,Cohort study ,medicine.medical_specialty ,Article ,NO ,03 medical and health sciences ,Phylogenetic analysi ,Virology ,Bioinformatic ,business.industry ,Public health ,Migrant ,Settore MED/17 ,030104 developmental biology ,Risk factor ,business ,Demography - Abstract
We aimed at evaluating the characteristics of HIV-1 molecular transmission clusters (MTCs) among natives and migrants living in Italy, diagnosed between 1998 and 2018. Phylogenetic analyses were performed on HIV-1 polymerase (pol) sequences to characterise subtypes and identify MTCs, divided into small (SMTCs, 2&ndash, 3 sequences), medium (MMTCs, 4&ndash, 9 sequences) and large (LMTCs, &ge, 10 sequences). Among 3499 drug-naï, ve individuals enrolled in the Italian Cohort Naive Antiretroviral (ICONA) cohort (2804 natives, 695 migrants), 726 (20.8%, 644 natives, 82 migrants) were involved in 228 MTCs (6 LMTCs, 36 MMTCs, 186 SMTCs). Migrants contributed 14.4% to SMTCs, 7.6% to MMTCs and 7.1% to LMTCs, respectively. HIV-1 non-B subtypes were found in 51 MTCs, noteworthy was that non-B infections involved in MTCs were more commonly found in natives (n = 47) than in migrants (n = 4). Factors such as Italian origin, being men who have sex with men (MSM), younger age, more recent diagnosis and a higher CD4 count were significantly associated with MTCs. Our findings show that HIV-1 clustering transmission among newly diagnosed individuals living in Italy is prevalently driven by natives, mainly MSM, with a more recent diagnosis and frequently infected with HIV-1 non-B subtypes. These results can contribute to monitoring of the HIV epidemic and guiding the public health response to prevent new HIV infections.
- Published
- 2020
24. The lowest X4 Geno2Pheno false-positive rate is associated with greater CD4 depletion in HIV-1 infected patients
- Author
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Santoro, M.M., Armenia, D., Fabeni, L., Santoro, M., Gori, C., Forbici, F., Svicher, V., Bertoli, A., Dori, L., Surdo, M., Balestra, E., Palamara, G., Girardi, E., Angarano, G., Andreoni, M., Narciso, P., Antinori, A., Ceccherini-Silberstein, F., and Perno, C.F.
- Published
- 2012
- Full Text
- View/download PDF
25. Serum HBsAg and ddPCR HBV-DNA as predictive parameters of HBsAg loss after nucleo(s)tide analogue (NA) treatment discontinuation in non-cirrhotic patients with Chronic Hepatitis B
- Author
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Guerra, A.F., primary, Tomassoli, G., additional, Piermatteo, L., additional, D'Anna, S., additional, Salpini, R., additional, Svicher, V., additional, Abbati, G., additional, Pietrangelo, A., additional, and Ventura, P., additional
- Published
- 2022
- Full Text
- View/download PDF
26. Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group
- Author
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Svicher, V., Alteri, C., Montano, M., Nori, A., D’Arrigo, R., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Allice, T., Bagnarelli, P., Baldanti, F., Bertoli, A., Borderi, M., Boeri, E., Bon, I., Bruzzone, B., Barresi, R., Calderisi, S., Callegaro, A. P., Capobianchi, M. R., Gargiulo, F., Castelli, F., Cauda, R., Ceccherini-Silberstein, F., Clementi, M., Chirianni, A., Colafigli, M., D’Arminio Monforte, A., De Luca, A., Di Biagio, A., Di Nicuolo, G., Di Perri, G., Di Santo, F., Fadda, G., Galli, M., Gennari, W., Ghisetti, V., Costantini, A., Gori, A., Gulminetti, R., Leoncini, F., Maffongelli, G., Maggiolo, F., Maserati, R., Mazzotta, F., Meini, G., Micheli, V., Monno, L., Mussini, C., Nozza, S., Paolucci, S., Palù, G., Parisi, S., Parruti, G., Pignataro, A. R., Quirino, T., Re, M. C., Rizzardini, G., Sanguinetti, M., Santangelo, R., Scaggiante, R., Sterrantino, G., Turriziani, O., Vatteroni, M. L., Viscoli, C., Vullo, V., Zazzi, M., Lazzarin, A., and Perno, C. F.
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- 2014
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27. HCV NS3 sequencing as a reliable and clinically useful tool for the assessment of genotype and resistance mutations for clinical samples with different HCV-RNA levels
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Di Maio, V. C., Cento, V., Di Paolo, D., Aragri, M., De Leonardis, F., Tontodonati, M., Micheli, V., Bellocchi, M. C., Antonucci, F. P., Bertoli, A., Lenci, I., Milana, M., Gianserra, L., Melis, M., Di Biagio, A., Sarrecchia, C., Sarmati, L., Landonio, S., Francioso, S., Lambiase, L., Nicolini, L. A., Marenco, S., Nosotti, L., Giannelli, V., Siciliano, M., Romagnoli, D., Pellicelli, A., Vecchiet, J., Magni, C. F., Babudieri, S., Mura, M. S., Taliani, G., Mastroianni, C., Vespasiani-Gentilucci, U., Romano, M., Morisco, F., Gasbarrini, A., Vullo, V., Bruno, S., Baiguera, C., Pasquazzi, C., Tisone, G., Picciotto, A., Andreoni, M., Parruti, G., Rizzardini, G., Angelico, M., Perno, C. F., Ceccherini-Silberstein, F., Mariani, R., Paoloni, M., Iapadre, N., Grimaldi, A., Menzaghi, B., Quirino, T., Vecchiet, J., Bruzzone, B., De Maria, A., Di Biagio, A., Marenco, S., Nicolini, L. A., Picciotto, A., Viscoli, C., Casinelli, K., Monache, M. Delle, Lichtner, M., Mastroianni, C., Aghemo, A., Bruno, S., Cerrone, M., Colombo, M., Monforte, A. DʼArminio, Danieli, E., Donato, F., Gubertini, G., Landonio, S., Magni, C. F., Mancon, A., Micheli, V., Monico, S., Niero, F., Puoti, M., Rizzardini, G., Russo, M. L., Alfieri, R., Gnocchi, M., Orro, A., Milanesi, L., Baldelli, E., Bertolotti, M., Borghi, V., Mussini, C., Romagnoli, D., Brancaccio, G., Caporaso, N., Gaeta, G. B., Lembo, V., Morisco, F., Calvaruso, V., Craxì, A., Di Marco, V., Mazzola, A., Petta, S., DʼAmico, E., Cacciatore, P., Consorte, A., Palitti, V. Pace, Parruti, G., Pieri, A., Polilli, E., Tontodonati, M., Andreoni, M., Angelico, M., Antenucci, F., Antonucci, F. P., Aragri, M., Armenia, D., Baiocchi, L., Bellocchi, M., Bertoli, A., Biliotti, E., Biolato, M., Carioti, L., Ceccherini-Silberstein, F., Cento, V., Cerasari, G., Cerva, C., Ciotti, M., DʼAmbrosio, C., DʼEttorre, G., De Leonardis, F., De Sanctis, A., Di Maio, V. C., Di Paolo, D., Francioso, S., Furlan, C., Gallo, P., Gasbarrini, A., Giannelli, V., Gianserra, L., Grieco, A., Grieco, S., Lambiase, L., Lattanzi, B., Lenci, I., Malagnino, V., Manuelli, M., Merli, M., Miglioresi, L., Milana, M., Nosotti, L., Palazzo, D., Pasquazzi, C., Pellicelli, A., Perno, C. F., Romano, M., Santopaolo, F., Santoro, M. M., Sarmati, L., Sarrecchia, C., Sforza, D., Siciliano, M., Sorbo, M. C., Spaziante, M., Svicher, V., Taliani, G., Teti, E., Tisone, G., Vespasiani-Gentilucci, U., Vullo, V., Mangia, A., Babudieri, S., Maida, I., Melis, M., Mura, M. S., Falconi, L., Di Giammartino, D., and Tarquini, P.
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- 2016
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28. Evidence of extensive transcriptionally active HBV integrations involving genetic regions crucial for cell proliferation in the setting of HBeAg positive infection
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Salpini, R, Carioti, L, Battisti, A, Piermatteo, L, Benedetti, L, Ceccherini-Silberstein, F, Gill, U, Svicher, V, and Kennedy, P
- Subjects
Hepatology ,Settore MED/07 - Published
- 2022
29. Persistent risk of HBV reactivation despite extensive lamivudine prophylaxis in haematopoietic stem cell transplant recipients who are anti-HBc-positive or HBV-negative recipients with an anti-HBc-positive donor
- Author
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Cerva, C., Colagrossi, L., Maffongelli, G., Salpini, R., Di Carlo, D., Malagnino, V., Battisti, A., Ricciardi, A., Pollicita, M., Bianchi, A., Picardi, A., Cudillo, L., Cerretti, R., De Angelis, G., Cantonetti, M., Andreoni, M., Perno, C.F., Arcese, W., Svicher, V., and Sarmati, L.
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- 2016
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30. HIV-1 integrase genotyping is reliable and reproducible for routine clinical detection of integrase resistance mutations even in patients with low-level viraemia
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Armenia, D., Fabeni, L., Alteri, C., Di Pinto, D., Di Carlo, D., Bertoli, A., Gori, C., Carta, S., Fedele, V., Forbici, F., DʼArrigo, R., Svicher, V., Berno, G., Pizzi, D., Nicastri, E., Sarmati, L., Pinnetti, C., Ammassari, A., DʼOffizi, G., Latini, A., Andreoni, M., Antinori, A., Ceccherini-Silberstein, F., Perno, C. F., and Santoro, M. M.
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- 2015
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31. HBcAb Positivity Increases the Risk of Severe Hepatic Fibrosis Development in HIV/HCV-Positive Subjects from the ICONA Italian Cohort of HIV-Infected Patients
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Malagnino, Vincenzo, Cerva, Carlotta, Cingolani, Antonella, Ceccherini-Silberstein, Francesca, Vergori, Alessandra, Cuomo, Gianluca, Perno, Carlo Federico, Puoti, Massimo, d'Arminio Monforte, Antonella, Cozzi-Lepri, Alessandro, Andreoni, Massimo, Sarmati, Loredana, d’Arminio Monforte (President), Icona Fundation Study Group. Board of Directors: A., Antinori (Vice-President), A., Andreoni, M., Castagna, A., Castelli, F., Cauda, R., Di Perri, G., Galli, M., Iardino, R., Ippolito, G., Lazzarin, A., Marchetti, G. C., Rezza, G., von Schloesser, F., d’Arminio Monforte, P. Viale. Scientific Secretary: A., Antinori, A., Ceccherinisilberstein, F., Cozzi-Lepri, A., Girardi, E., Gori, A., Lo Caputo, S., Maggiolo, F., Mussini, C., Puoti, M., Antinori, C. F. Perno. Steering Committee: A., Bai, F., Bandera, A., Bonora, S., Borderi, M., Calcagno, A., Capobianchi, M. R., Ceccherini-Silberstein, F., Cicalini, S., Cingolani, A., Cinque, P., d’Arminio Monforte, A., Di Biagio, A., Gagliardini, R., Gianotti, N., Guaraldi, G., Lapadula, G., Lichtner, M., Lai, A., Madeddu, G., Marchetti, G., Merlini, E., Nozza, S., Perno, C. F., Piconi, S., Pinnetti, C., Quiros Roldan, E., Rossotti, R., Rusconi, S., Santoro, M. M., Saracino, A., Sarmati, L., Spagnuolo, V., Svicher, V., Taramasso, L., Cozzi-Lepri, Statistical and Monitoring Team: A., Fanti, I., Galli, L., Lorenzini, P., Rodanó, A., Macchia, M., Bove, A. Tavelli. Community Advisory Board: A., Camposeragna, A., Errico, M., Manfredini, M., Perziano, A., Carletti, V. Calvino. Biological Bank INMI: F., Carrara, S., Di Caro, A., Graziano, S., Petroni, F., Prota, G., Giacometti, S. Truffa. Participating Physicians and Centers: Italy: A., Costantini, A., Barocci (Ancona), V., Angarano, G., Monno, L., Milano (Bari), E., F. Maggiolo, C. Suardi (Bergamo), Viale, P., Donati, V., Verucchi (Bologna), G., Castelnuovo, F., Minardi, C., Quiros Roldan (Brescia), E., B. Menzaghi, C. Abeli (Busto Arsizio), L. Chessa, F. Pes (Cagliarti), B. Cacopardo, B. Celesia (Catania), J. Vecchiet, K. Falasca (Chieti), A. Pan, S. Lorenzotti (Cremona), L. Sighinolfi, D. Segala (Ferrara), P. Blanc, F. Vichi (Firenze), Cassola, G., Bassetti, M., Alessandrini, A., Bobbio, N., Mazzarello (Genova), G., M. Lichtner, L. Fondaco (Latina), P. Bonfanti, C. Molteni (Lecco), A. Chiodera, P. Milini (Macerata), G. Nunnari, G. Pellicanò (Messina), Rizzardini, G., Cannizzo, E. S., Moioli, M. C., Piolini, R., Bernacchia, D., Poli, A., Tincati (Milano), C., C. Mussini, C. Puzzolante (Modena), C. Migliorino, G. Lapadula (Monza), Sangiovanni, V., Borgia, G., Esposito, V., Di Flumeri, G., Gentile, I., Rizzo (Napoli), V., A. M. Cattelan, S. Marinello (Padova), A. Cascio, M. Trizzino (Palermo), D. Francisci, E. Schiaroli (Perugia), G. Parruti, F. Sozio (Pescara), C. Lazzaretti, R. Corsini (Reggio Emilia), Cristaudo, A., Vullo, V., Acinapura, R., Lamonica, S., Capozzi, M., Mondi, A., Rivano Capparuccia, M., Iaiani, G., Latini, A., Onnelli, G., Plazzi, M. M., De Girolamo, G., Vergori (Roma), A., M. Cecchetto, F. Viviani (Rovigo), G. Madeddu, A. De Vito (Sassari), B. Rossetti, F. Montagnani (Siena), A. Franco, R. Fontana Del Vecchio (Siracusa), Di Giuli (Terni), C., Caramello, P., Orofino, G. C., Sciandra (Torino), M., Londero (Udine), A., V. Manfrin, G. Battagin (Vicenza), G. Starnini, A. Ialungo (Viterbo)., Malagnino, V, Cerva, C, Cingolani, A, Ceccherini-Silberstein, F, Vergori, A, Cuomo, G, Perno, C, Puoti, M, D'Arminio Monforte, A, Cozzi-Lepri, A, Andreoni, M, Sarmati, L, Malagnino, V., Cerva, C., Cingolani, A., Ceccherini-Silberstein, F., Vergori, A., Cuomo, G., Perno, C. F., Puoti, M., D'Arminio Monforte, A., Cozzi-Lepri, A., Andreoni, M., Sarmati, L., ICONA Foundation Study, Group, Castagna, A., Malagnino V., Cerva C., Cingolani A., Ceccherini-Silberstein F., Vergori A., Cuomo G., Perno C.F., Puoti M., D'Arminio Monforte A., Cozzi-Lepri A., Andreoni M., and Sarmati L.A Castagna, F Castelli, R Cauda, G Di Perri, M Galli, R Iardino, G Ippolito, A Lazzarin, G C Marchetti, G Rezza, F von Schloesser, E Girardi, A Gori, S Lo Caputo, F Maggiolo, F Bai, A Bandera, S Bonora, M Borderi, A Calcagno, M R Capobianchi, S Cicalini, P Cinque, A Di Biagio, R Gagliardini, N Gianotti, G Guaraldi, G Lapadula, M Lichtner, A Lai, S Lo Caputo, G Madeddu, G Marchetti, E Merlini, C Mussini, S Nozza, S Piconi, C Pinnetti, E Quiros Roldan, R Rossotti, S Rusconi, M M Santoro, A Saracino, V Spagnuolo, V Svicher, L Taramasso, I Fanti, L Galli, P Lorenzini, A Rodanó, M Macchia, A Tavelli, A Bove, A Camposeragna, M Errico, M Manfredini, A Perziano, V Calvino, F Carletti, S Carrara, A Di Caro, S Graziano, F Petroni, G Prota, S Truffa, A Giacometti, A Costantini, V Barocci, G Angarano, L Monno, E Milano, C Suardi, V Donati, G Verucchi, F Castelnuovo, C Minardi, B Menzaghi, C Abeli, L Chessa, F Pes, B Cacopardo, B Celesia, J Vecchiet, K Falasca, A Pan, S Lorenzotti, L Sighinolfi, D Segala, P Blanc, F Vichi, G Cassola, M Bassetti, A Alessandrini, N Bobbio, G Mazzarello, M Lichtner, L Fondaco, P Bonfanti, C Molteni, A Chiodera, P Milini, G Nunnari, G Pellicanò, , G Rizzardini, E S Cannizzo, M C Moioli, R Piolini, D Bernacchia, A Poli, C Tincati, C Puzzolante, C Migliorino, G Lapadula, V Sangiovanni, G Borgia, V Esposito, G Di Flumeri, I Gentile, V Rizzo, A M Cattelan, S Marinello, A Cascio, M Trizzino, D Francisci, E Schiaroli, G Parruti, F Sozio, C Lazzaretti, R Corsini, A Antinori, A Cristaudo, V Vullo, R Acinapura, S Lamonica, M Capozzi, A Mondi, M Rivano Capparuccia, G Iaiani, A Latini, G Onnelli, M M Plazzi, G De Girolamo, M Cecchetto, F Viviani, G Madeddu, A De Vito, B Rossetti, F Montagnani, A Franco, R Fontana Del Vecchio, C Di Giuli, P Caramello, G C Orofino, M Sciandra, A Londero, V Manfrin, G Battagin, G Starnini, A Ialungo
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HBsAg ,medicine.medical_specialty ,Hepatitis C virus ,Liver fibrosis ,Human immunodeficiency virus (HIV) ,OBI ,medicine.disease_cause ,anti-HBc ,HBV ,HIV/HBV coinfection ,liver fibrosis ,Gastroenterology ,Major Articles ,NO ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Hiv infected patients ,030212 general & internal medicine ,Hepatitis B virus ,business.industry ,liver fibrosi ,virus diseases ,Settore MED/17 ,digestive system diseases ,Anti-HBc, HIV-HBV coinfection, HBV, Liver fibrosis, OBI ,Infectious Diseases ,AcademicSubjects/MED00290 ,HIV-HBV coinfection ,Oncology ,Cohort ,030211 gastroenterology & hepatology ,business ,Hepatic fibrosis - Abstract
Background The aim of this study was to investigate the impact of anti-HBc (HBcAb) positivity on the progression of liver fibrosis (Fibrosis-4 score >3.25) in the Italian cohort of HIV-infected individuals naïve to antiretroviral treatment (ICONA). Methods All patients with FIB-4 Results Patients who were HBcAb+/HCV-/HBs antigen (HBsAg)- and HCV+/HBcAb+/HBsAg- or HBsAg+/HBcAb+/HCV- had CD4+ cell counts below the nadir and significantly higher prevalence of AIDS diagnosis at baseline than the other groups (P < .0001). A Cox regression model adjusted for age, HIV transmission mode, country of birth, and alcohol consumption showed a higher relative risk (HR) of progression to FIB-4 >3.25 in HCV+/HBcAb+/HBsAg- patients (HR, 7.2; 95% CI, 3 8–13.64). Conclusions HBcAb+ contributes to liver damage in HIV+/HCV+/HBcAb+/HBsAg- subjects. A careful monitoring for signs of previous HBV infection is needed in this kind of patients.
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- 2021
32. Successful switch to tenofovir after suboptimal response to entecavir in an immunocompromised patient with chronic hepatitis B and without genotypic hepatitis B virus resistance
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Sarrecchia, C., Svicher, V., Volpi, A., Salpini, R., Ceccarelli, L., Sordillo, P., Bertoli, A., Perno, C. F., and Andreoni, M.
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- 2011
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33. Genetic Determinants in a Critical Domain of NS5A Correlate with Hepatocellular Carcinoma in Cirrhotic Patients Infected with HCV Genotype 1b
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Alkhatib M, Di Maio VC, De Murtas V, Polilli E, Milana M, Teti E, Fiorentino G, Calvaruso V, Barbaliscia S, Bertoli A, Scutari R, Carioti L, Cento V, Santoro MM, Orro A, Maida I, Lenci I, Sarmati L, Craxi A, Pasquazzi C, Parruti G, Babudieri S, Milanesi L, Andreoni M, Angelico M, Perno CF, Ceccherini-Silberstein F, Svicher V, Salpini R, On Behalf Of Hirma Hepatocarcinoma Innovative Research MArkers And Fondazione Vironet C Hcv Virology Italian Resistance., and Alkhatib M, Di Maio VC, De Murtas V, Polilli E, Milana M, Teti E, Fiorentino G, Calvaruso V, Barbaliscia S, Bertoli A, Scutari R, Carioti L, Cento V, Santoro MM, Orro A, Maida I, Lenci I, Sarmati L, Craxi A, Pasquazzi C, Parruti G, Babudieri S, Milanesi L, Andreoni M, Angelico M, Perno CF, Ceccherini-Silberstein F, Svicher V, Salpini R, On Behalf Of Hirma Hepatocarcinoma Innovative Research MArkers And Fondazione Vironet C Hcv Virology Italian Resistance.
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hepatitis C virus ,Liver Cirrhosis ,Male ,Cirrhosis ,viruses ,Hepacivirus ,Viral Nonstructural Proteins ,NS5A ,medicine.disease_cause ,Severity of Illness Index ,genetic variability ,Medicine ,Liver Neoplasms ,virus diseases ,hepatocellular carcinoma ,Middle Aged ,Hepatitis C ,QR1-502 ,Infectious Diseases ,Hepatocellular carcinoma ,HCV ,Host-Pathogen Interactions ,Female ,Disease Susceptibility ,Carcinoma, Hepatocellular ,Genotype ,Hepatitis C virus ,Viremia ,Microbiology ,Article ,Structure-Activity Relationship ,Virology ,Genetic variation ,Humans ,Genetic variability ,neoplasms ,Aged ,business.industry ,cirrhosis ,Sequence Analysis, DNA ,biochemical phenomena, metabolism, and nutrition ,genotype 1b ,medicine.disease ,Settore MED/17 ,digestive system diseases ,Mutation ,Cancer research ,business ,Carcinogenesis ,Biomarkers - Abstract
HCV is an important cause of hepatocellular carcinoma (HCC). HCV NS5A domain-1 interacts with cellular proteins inducing pro-oncogenic pathways. Thus, we explore genetic variations in NS5A domain-1 and their association with HCC, by analyzing 188 NS5A sequences from HCV genotype-1b infected DAA-naïve cirrhotic patients: 34 with HCC and 154 without HCC. Specific NS5A mutations significantly correlate with HCC: S3T (8.8% vs. 1.3%, p = 0.01), T122M (8.8% vs. 0.0%, p <, 0.001), M133I (20.6% vs. 3.9%, p <, 0.001), and Q181E (11.8% vs. 0.6%, p <, 0.001). By multivariable analysis, the presence of >, 1 of them independently correlates with HCC (OR (95%CI): 21.8 (5.7–82.3), p <, 0.001). Focusing on HCC-group, the presence of these mutations correlates with higher viremia (median (IQR): 5.7 (5.4–6.2) log IU/mL vs. 5.3 (4.4–5.6) log IU/mL, p = 0.02) and lower ALT (35 (30–71) vs. 83 (48–108) U/L, p = 0.004), suggesting a role in enhancing viral fitness without affecting necroinflammation. Notably, these mutations reside in NS5A regions known to interact with cellular proteins crucial for cell-cycle regulation (p53, p85-PIK3, and β-catenin), and introduce additional phosphorylation sites, a phenomenon known to ameliorate NS5A interaction with cellular proteins. Overall, these results provide a focus for further investigations on molecular bases of HCV-mediated oncogenesis. The role of theseNS5A domain-1 mutations in triggering pro-oncogenic stimuli that can persist also despite achievement of sustained virological response deserves further investigation.
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- 2021
34. Successful ongoing retreatment with glecaprevir/pibrentasvir + sofosbuvir + ribavirin in a patient with HCV genotype 3 who failed glecaprevir/pibrentasvir with both NS3 and NS5A resistance
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Aragri, M., Milana, M., Di Maio, V.C., Lenci, I., Carioti, L., Perno, C.F., Svicher, V., Angelico, M., and Ceccherini-Silberstein, F.
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- 2020
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35. Impact of universal art access on Kaposi Sarcoma: results from the ICONA Cohort
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Bandera, A., Cozzi-Lepri, A., Cingolani, A., Ceccherini Silberstein, F., Marchetti, G., Rusconi, S., Svicher, V., Cauda, R., Castelli, F., Gori, A., Antinori, A., d’Arminio Monforte, A., for the ICONA Founfation Study Group, Nunnari, G., and Pellicano', Giovanni Francesco
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- 2020
36. Snapshot on drug-resistance rate and profiles in patients with chronic hepatitis B receiving nucleos(t)ide analogues in clinical practice1
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Salpini, R., Alteri, C., Cento, V., Pollicita, M., Micheli, V., Gubertini, G., De Sanctis, G. M., Visca, M., Romano, S., Sarrecchia, C., Andreoni, M., Angelico, M., Parruti, G., Svicher, V., and Perno, C. F.
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- 2013
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37. Characterization of drug-resistance mutations in HBV D-genotype chronically infected patients, naïve to antiviral drugs
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Salpini, R., Svicher, V., Cento, V., Gori, C., Bertoli, A., Scopelliti, F., Micheli, V., Cappiello, T., Spanò, A., Rizzardini, G., De Sanctis, G. M., Sarrecchia, C., Angelico, M., and Perno, C. F.
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- 2011
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38. The combined usage of accurate virological and serological HBV markers can help to identify HBsAg-negative/anti-HBc-positive patients at higher risk of HBV-reactivation and to optimize prophylaxis duration in oncohematological setting
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Salpini, R., primary, Alkhatib, M., additional, Cerva, C., additional, Piermatteo, L., additional, Battisti, A., additional, Malagnino, V., additional, Fabeni, L, additional, Cantonetti, M.L., additional, Arcese, W., additional, Ceccherini-Silberstein, F., additional, Perno, C.F., additional, Andreoni, M., additional, Sarmati, L., additional, and Svicher, V., additional
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- 2020
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39. Quantitative HBeAg varies across the different phases of HBV infection, and can predict treatment outcome in the setting of HBV-reactivation driven by iatrogenic immunosuppression
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Piermatteo, L., primary, Salpini, R., additional, Alkhatib, M., additional, Bertoli, A., additional, Iuvara, A., additional, De Cristofaro, M., additional, Cappiello, G., additional, Cerva, C., additional, Minichini, C., additional, Pisaturo, M., additional, Starace, M., additional, Coppola, N., additional, Fontana, C., additional, Sarmati, L., additional, Andreoni, M., additional, Angelico, M., additional, Grelli, S., additional, Silberstein, F. Ceccherini, additional, Perno, C.F., additional, and Svicher, V., additional
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- 2020
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40. HCV NS3 sequencing as a reliable and clinically useful tool for the assessment of genotype and resistance mutations for clinical samples with different HCV-RNA levels
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Di Maio, V C, Cento, V, Di Paolo, D, Aragri, M, De Leonardis, F, Tontodonati, M, Micheli, V, Bellocchi, M C, Antonucci, F P, Bertoli, A, Lenci, I, Milana, M, Gianserra, L, Melis, M, Di Biagio, A, Sarrecchia, C, Sarmati, L, Landonio, S, Francioso, S, Lambiase, L, Nicolini, L A, Marenco, S, Nosotti, L, Giannelli, V, Siciliano, M, Romagnoli, D, Pellicelli, A, Vecchiet, J, Magni, C F, Babudieri, S, Mura, M S, Taliani, G, Mastroianni, C, Vespasiani-Gentilucci, U, Romano, M, Morisco, F, Gasbarrini, A, Vullo, V, Bruno, S, Baiguera, C, Pasquazzi, C, Tisone, G, Picciotto, A, Andreoni, M, Parruti, G, Rizzardini, G, Angelico, M, Perno, C F, Ceccherini-Silberstein, F, Collaborators (129) Mariani R, HCV Italian Resistance Network Study Group., Paoloni, M, Iapadre, N, Grimaldi, A, Menzaghi, B, Quirino, T, Bruzzone, B, De Maria, A, Nicolini, La, Viscoli, C, Casinelli, K, Monache, Md, Lichtner, M, Aghemo, A, Cerrone, M, Colombo, M, Monforte, Ad, Danieli, E, Donato, F, Gubertini, G, Magni, Cf, Mancon, A, Monico, S, Niero, F, Puoti, M, Russo, Ml, Alfieri, R, Gnocchi, M, Orro, A, Milanesi, L, Baldelli, E, Bertolotti, M, Borghi, V, Mussini, C, Brancaccio, G, Caporaso, N, Gaeta, Gb, Lembo, V, Calvaruso, V, Craxì, A, Di Marco, V, Mazzola, A, Petta, S, D'Amico, E, Cacciatore, P, Consorte, A, Palitti, Vp, Pieri, A, Polilli, E, Antenucci, F, Antonucci, Fp, Armenia, D, Baiocchi, L, Bellocchi, M, Biliotti, E, Biolato, M, Carioti, L, Cerasari, G, Cerva, C, Ciotti, M, D'Ambrosio, C, D'Ettorre, G, De Sanctis, A, Di Maio VC, Furlan, C, Gallo, P, Grieco, A, Grieco, S, Lattanzi, B, Malagnino, V, Manuelli, M, Merli, M, Miglioresi, L, Palazzo, D, Perno, Cf, Santopaolo, F, Santoro, Mm, Sforza, D, Sorbo, Mc, Spaziante, M, Svicher, V, Teti, E, Mangia, A, Maida, I, Mura, Ms, Falconi, L, Di Giammartino, D, Tarquini, P., Di Maio, V C, Cento, V, Di Paolo, D, Aragri, M, De Leonardis, F, Tontodonati, M, Micheli, V, Bellocchi, M C, Antonucci, F P, Bertoli, A, Lenci, I, Milana, M, Gianserra, L, Melis, M, Di Biagio, A, Sarrecchia, C, Sarmati, L, Landonio, S, Francioso, S, Lambiase, L, Nicolini, L A, Marenco, S, Nosotti, L, Giannelli, V, Siciliano, M, Romagnoli, D, Pellicelli, A, Vecchiet, J, Magni, C F, Babudieri, S, Mura, M S, Taliani, G, Mastroianni, C, Vespasiani-Gentilucci, U, Romano, M, Morisco, F, Gasbarrini, A, Vullo, V, Bruno, S, Baiguera, C, Pasquazzi, C, Tisone, G, Picciotto, A, Andreoni, M, Parruti, G, Rizzardini, G, Angelico, M, Perno, C F, Ceccherini-Silberstein, F, HCV Italian Resistance Network Study Group., Collaborators (129) Mariani R, Paoloni, M, Iapadre, N, Grimaldi, A, Menzaghi, B, Quirino, T, Bruzzone, B, De Maria, A, Nicolini, La, Viscoli, C, Casinelli, K, Monache, Md, Lichtner, M, Aghemo, A, Cerrone, M, Colombo, M, Monforte, Ad, Danieli, E, Donato, F, Gubertini, G, Magni, Cf, Mancon, A, Monico, S, Niero, F, Puoti, M, Russo, Ml, Alfieri, R, Gnocchi, M, Orro, A, Milanesi, L, Baldelli, E, Bertolotti, M, Borghi, V, Mussini, C, Brancaccio, G, Caporaso, N, Gaeta, Gb, Lembo, V, Calvaruso, V, Craxì, A, Di Marco, V, Mazzola, A, Petta, S, D'Amico, E, Cacciatore, P, Consorte, A, Palitti, Vp, Pieri, A, Polilli, E, Antenucci, F, Antonucci, Fp, Armenia, D, Baiocchi, L, Bellocchi, M, Biliotti, E, Biolato, M, Carioti, L, Cerasari, G, Cerva, C, Ciotti, M, D'Ambrosio, C, D'Ettorre, G, De Sanctis, A, Di Maio, Vc, Furlan, C, Gallo, P, Grieco, A, Grieco, S, Lattanzi, B, Malagnino, V, Manuelli, M, Merli, M, Miglioresi, L, Palazzo, D, Perno, Cf, Santopaolo, F, Santoro, Mm, Sforza, D, Sorbo, Mc, Spaziante, M, Svicher, V, Teti, E, Mangia, A, Maida, I, Mura, M, Falconi, L, Di Giammartino, D, Tarquini, P., Di Maio, V. C, Bellocchi, M. C, Antonucci, F. P, Nicolini, L. A, Magni, C. F, Mura, M. S, Vespasiani Gentilucci, U, Morisco, Filomena, Perno, C. F, Ceccherini Silberstein, F., Caporaso, Nicola, Di Maio, V., Cento, V., Di Paolo, D., Aragri, M., De Leonardis, F., Tontodonati, M., Micheli, V., Bellocchi, M., Antonucci, F., Bertoli, A., Lenci, I., Milana, M., Gianserra, L., Melis, M., Di Biagio, A., Sarrecchia, C., Sarmati, L., Landonio, S., Francioso, S., Lambiase, L., Nicolini, L., Marenco, S., Nosotti, L., Giannelli, V., Siciliano, M., Romagnoli, D., Pellicelli, A., Vecchiet, J., Magni, C., Babudieri, S., Mura, M., Taliani, G., Mastroianni, C., Vespasiani-Gentilucci, U., Romano, M., Morisco, F., Gasbarrini, A., Vullo, V., Bruno, S., Baiguera, C., Pasquazzi, C., Tisone, G., Picciotto, A., Andreoni, M., Parruti, G., Rizzardini, G., Angelico, M., Perno, C., Ceccherini-Silberstein, F., Mariani, R., Paoloni, M., Iapadre, N., Grimaldi, A., Menzaghi, B., Quirino, T., Bruzzone, B., De Maria, A., Viscoli, C., Casinelli, K., Delle Monache, M., Lichtner, M., Aghemo, A., Cerrone, M., Colombo, M., D'Arminio Monforte, A., Danieli, E., Donato, F., Gubertini, G., Mancon, A., Monico, S., Niero, F., Puoti, M., Russo, M., Alfieri, R., Gnocchi, M., Orro, A., Milanesi, L., Baldelli, E., Bertolotti, M., Borghi, V., Mussini, C., Brancaccio, G., Caporaso, N., Gaeta, G., Lembo, V., Calvaruso, V., Craxã, A., DI MARCO, V., Mazzola, A., Petta, S., D'Amico, E., Cacciatore, P., Consorte, A., Pace Palitti, V., Pieri, A., Polilli, E., Antenucci, F., Armenia, D., Baiocchi, L., Biliotti, E., Biolato, M., Carioti, L., Cerasari, G., Cerva, C., Ciotti, M., D'Ambrosio, C., D'Ettorre, G., De Sanctis, A., Furlan, C., Gallo, P., Grieco, A., Grieco, S., Lattanzi, B., Malagnino, V., Manuelli, M., Merli, M., Miglioresi, L., Palazzo, D., Santopaolo, F., Santoro, M., Sforza, D., Sorbo, M., Spaziante, M., Svicher, V., Teti, E., Mangia, A., Maida, I., Falconi, L., and Di Giammartino, D.
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0301 basic medicine ,ns3 ,Genotyping Techniques ,viruses ,Drug Resistance ,Hepacivirus ,Viral Nonstructural Proteins ,medicine.disease_cause ,Gastroenterology ,Telaprevir ,chemistry.chemical_compound ,genotype ,genotyping techniques ,hepacivirus ,hepatitis C ,humans ,RNA viral ,retrospective studies ,sequence analysis ,DNA ,viral nonstructural proteins ,drug resistance, viral ,mutation ,pharmacology ,infectious diseases ,0302 clinical medicine ,Retrospective Studie ,Genotype ,Pharmacology (medical) ,Viral ,Hepatitis C ,Humans ,RNA, Viral ,Retrospective Studies ,Sequence Analysis, DNA ,Drug Resistance, Viral ,Mutation ,Proteolytic enzymes ,virus diseases ,Settore MED/07 - Microbiologia e Microbiologia Clinica ,hcv-rna levels ,Infectious Diseases ,HCV-RNA ,030211 gastroenterology & hepatology ,Sequence Analysis ,medicine.drug ,Human ,Microbiology (medical) ,medicine.medical_specialty ,Hepatitis C virus ,Concordance ,Settore MED/12 - GASTROENTEROLOGIA ,Pharmacology ,Biology ,03 medical and health sciences ,Boceprevir ,Internal medicine ,medicine ,hcv ,Genotyping ,Hepaciviru ,Viral Nonstructural Protein ,Settore MED/09 - MEDICINA INTERNA ,Virology ,digestive system diseases ,030104 developmental biology ,chemistry ,Sequence Analysi ,RNA ,Genotyping Technique - Abstract
OBJECTIVES: This study aims to evaluate the reliability and clinical utility of NS3 sequencing in hepatitis C virus (HCV) 1-infected patients who were candidates to start a PI-containing regimen. METHODS: NS3 protease sequencing was performed by in-house-developed HCV-1 subtype-specific protocols. Phylogenetic analysis was used to test sequencing reliability and concordance with previous genotype/subtype assignment by commercial genotyping assays. RESULTS: Five hundred and sixty-seven HCV plasma samples with quantifiable HCV-RNA from 326 HCV-infected patients were collected between 2011 and 2014. Overall, the success rate of NS3 sequencing was 88.9%. The success rate between the two subtype protocols (HCV-1a/HCV-1b) was similarly high for samples with HCV-RNA >3 log IU/mL (>92% success rate), while it was slightly lower for HCV-1a samples with HCV-RNA ≤3 log IU/mL compared with HCV-1b samples. Phylogenetic analysis confirmed the genotype/subtype given by commercial genotyping assays in 92.9% (303/326) of cases analysed. In the remaining 23 cases (7.1%), 1 was HCV-1g (previously defined as subtype 1a), 1 was HCV-4d (previously defined as genotype 1b) and 1 was HCV-1b (previously defined as genotype 2a/2c). In the other cases, NS3 sequencing precisely resolved the either previous undetermined/discordant subtype 1 or double genotype/subtype assignment by commercial genotyping assays. Resistance-associated variants (RAVs) to PI were detected in 31.0% of samples. This prevalence changed according to PI experience (17.1% in PI-naive patients versus 79.2% in boceprevir/telaprevir/simeprevir-failing patients). Among 96 patients with available virological outcome following boceprevir/telaprevir treatment, a trend of association between baseline NS3 RAVs and virological failure was observed (particularly for HCV-1a-infected patients: 3/21 failing patients versus 0/22 achieving sustained virological response; P = 0.11). CONCLUSIONS: HCV-NS3 sequencing provides reliable results and at the same time gives two clinically relevant pieces of information: a correct subtype/genotype assignment and the detection of variants that may interfere with the efficacy of PI.
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- 2016
41. Impact Study Group. Novelties in Evaluation and Monitoring of Human Immunodeficiency Virus-1 Infection: Is Standard Virological Suppression Enough for Measuring Antiretroviral Treatment Success?
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Svicher V, Marchetti G, Ammassari A, Ceccherini-Silberstein F, Sarmati L, and, in the group, Maggi P, Svicher, V, Marchetti, G, Ammassari, A, Ceccherini-Silberstein, F, Sarmati, L, And, in the, Group, and Maggi, P
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- 2017
42. Multiclass HCV resistance to direct-acting antiviral failure in real-life patients advocates for tailored second-line therapies
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Di Maio, Velia C., Cento, Valeria, Lenci, Ilaria, Aragri, Marianna, Rossi, Piera, Barbaliscia, Silvia, Melis, Michela, Verucchi, Gabriella, Magni, Carlo F., Teti, Elisabetta, Bertoli, Ada, Antonucci, Francescopaolo, Bellocchi, Maria C., Micheli, Valeria, Masetti, Chiara, Landonio, Simona, Francioso, Simona, Santopaolo, Francesco, Pellicelli, Adriano M., Calvaruso, Vincenza, Gianserra, Laura, Siciliano, Massimo, Romagnoli, Dante, Cozzolongo, Raffaele, Grieco, Antonio, Vecchiet, Jacopo, Morisco, Filomena, Merli, Manuela, Brancaccio, Giuseppina, Di Biagio, Antonio, Loggi, Elisabetta, Mastroianni, Claudio Maria, Pace Palitti, Valeria, Tarquini, Pierluigi, Puoti, Massimo, Taliani, Gloria, Sarmati, Loredana, Picciotto, Antonino, Vullo, Vincenzo, Caporaso, Nicola, Paoloni, Maurizio, Pasquazzi, Caterina, Rizzardini, Giuliano, Parruti, Giustino, Craxì, Antonio, Babudieri, Sergio, Andreoni, Massimo, Angelico, Mario, Perno, Carlo F., Ceccherini Silberstein, Francesca, Mariani, R., Iapadre, N., Grimaldi, A., Cozzolongo, R., Andreone, P., Verucchi, G., Menzaghi, B., Quirino, T., Pisani, V., Torti, MARIA CHIARA, Vecchiet, J., Bruzzone, B., De Maria, A., Marenco, S., Nicolini, L. A., Viscoli, C., Casinelli, K., Delle Monache, M., Lichtner, Miriam, Aghemo, A., Boccaccio, V., Bruno, S., Cerrone, M., Colombo, M., D'Arminio Monforte, A., Danieli, E., Donato, F., Gubertini, G., Lleo, A., Magni, C. F., Mancon, A., Monico, S., Niero, F., Russo, M. L., Gnocchi, M., Orro, A., Milanesi, L., Baldelli, E., Bertolotti, M., Borghi, V., Mussini, C., Brancaccio, G., Gaeta, G. B., Lembo, V., Sangiovanni, V., Di Marco, V., Mazzola, A., Petta, S., D'Amico, E., Cacciatore, P., Consorte, A., Pieri, A., Polilli, E., Sozio, F., Antenucci, F., Aragri, M., Baiocchi, L., Barbaliscia, S., Biliotti, Elisa, Biolato, M., Carioti, L., Ceccherini Silberstein, F., Cerasari, G., Cerva, C., Ciotti, M., D'Ambrosio, C., D'Ettorre, G., De Leonardis, F., De Sanctis, A., Di Maio, V. C., Di Paolo, D., Furlan, Caterina, Gallo, P., Gasbarrini, A., Giannelli, V., Grieco, S., Lambiase, L., Lattanzi, B., Lenci, I., Lula, R., Malagnino, V., Manuelli, M., Miglioresi, L., Milana, M., Moretti, A., Nosotti, L., Palazzo, Donatella, Pellicelli, A., Romano, M., Sarrecchia, C., Sforza, D., Sorbo, M. C., Spaziante, M., Svicher, V., Tisone, G., Vespasiani Gentilucci, U., D'Adamo, G., Mangia, A., Maida, I., Mura, M. S., Falconi, L., Di Giammartino, D., Di Maio, V., Cento, V., Lenci, I., Aragri, M., Rossi, P., Barbaliscia, S., Melis, M., Verucchi, G., Magni, C., Teti, E., Bertoli, A., Antonucci, F., Bellocchi, M., Micheli, V., Masetti, C., Landonio, S., Francioso, S., Santopaolo, F., Pellicelli, A., Calvaruso, V., Gianserra, L., Siciliano, M., Romagnoli, D., Cozzolongo, R., Grieco, A., Vecchiet, J., Morisco, F., Merli, M., Brancaccio, G., Di Biagio, A., Loggi, E., Mastroianni, C., Pace Palitti, V., Tarquini, P., Puoti, M., Taliani, G., Sarmati, L., Picciotto, A., Vullo, V., Caporaso, N., Paoloni, M., Pasquazzi, C., Rizzardini, G., Parruti, G., Craxã¬, A., Babudieri, S., Andreoni, M., Angelico, M., Perno, C., Ceccherini-Silberstein, F., Mariani, R., Iapadre, N., Grimaldi, A., Andreone, P., Menzaghi, B., Quirino, T., Pisani, V., Torti, C., Bruzzone, B., De Maria, A., Marenco, S., Nicolini, L., Viscoli, C., Casinelli, K., Delle Monache, M., Lichtner, M., Aghemo, A., Boccaccio, V., Bruno, S., Cerrone, M., Colombo, M., D'Arminio Monforte, A., Danieli, E., Donato, F., Gubertini, G., Lleo, A., Mancon, A., Monico, S., Niero, F., Russo, M., Gnocchi, M., Orro, A., Milanesi, L., Baldelli, E., Bertolotti, M., Borghi, V., Mussini, C., Gaeta, G., Lembo, V., Sangiovanni, V., DI MARCO, V., Mazzola, A., Petta, S., D'Amico, E., Cacciatore, P., Consorte, A., Pieri, A., Polilli, E., Sozio, F., Antenucci, F., Baiocchi, L., Biliotti, E., Biolato, M., Carioti, L., Cerasari, G., Cerva, C., Ciotti, M., D'Ambrosio, C., D'Ettorre, G., De Leonardis, F., De Sanctis, A., Di Paolo, D., Furlan, C., Gallo, P., Gasbarrini, A., Giannelli, V., Grieco, S., Lambiase, L., Lattanzi, B., Lula, R., Malagnino, V., Manuelli, M., Miglioresi, L., Milana, M., Moretti, A., Nosotti, L., Palazzo, D., Romano, M., Sarrecchia, C., Sforza, D., Sorbo, M., Spaziante, M., Svicher, V., Tisone, G., Vespasiani-Gentilucci, U., D'Adamo, G., Mangia, A., Maida, I., Mura, M., Falconi, L., Di Giammartino, D., Di Maio, V, Cento, V, Lenci, I, Aragri, M, Rossi, P, Barbaliscia, S, Melis, M, Verucchi, G, Magni, C, Teti, E, Bertoli, A, Antonucci, F, Bellocchi, M, Micheli, V, Masetti, C, Landonio, S, Francioso, S, Santopaolo, F, Pellicelli, A, Calvaruso, V, Gianserra, L, Siciliano, M, Romagnoli, D, Cozzolongo, R, Grieco, A, Morisco, F, Merli, M, Brancaccio, G, Di Biagio, A, Loggi, E, Mastroianni, C, Pace Palitti, V, Tarquini, P, Puoti, M, Taliani, G, Sarmati, L, Picciotto, A, Vullo, V, Caporaso, N, Paoloni, M, Pasquazzi, C, Rizzardini, G, Parruti, G, Craxì, A, Babudieri, S, Andreoni, M, Angelico, M, Perno, C, Ceccherini-Silberstein, F, Velia C. Di Maio, Valeria Cento, Ilaria Lenci, Marianna Aragri, Piera Rossi, Silvia Barbaliscia, Michela Meli, Gabriella Verucchi, Carlo F. Magni, Elisabetta Teti, Ada Bertoli, Francesco Paolo Antonucci, Maria C. Bellocchi, Valeria Micheli, Chiara Masetti, Simona Landonio, Simona Francioso, Francesco Santopaolo, Adriano M. Pellicelli, Vincenza Calvaruso, Laura Gianserra, Massimo Siciliano, Dante Romagnoli, Raffaele Cozzolongo, Antonio Grieco, Jacopo Vecchiet, Filomena Morisco, Manuela Merli, Giuseppina Brancaccio, Antonio Di Biagio, Elisabetta Loggi, Claudio M. Mastroianni, Valeria Pace Palitti, Pierluigi Tarquini, Massimo Puoti, Gloria Taliani, Loredana Sarmati, Antonino Picciotto, Vincenzo Vullo, Nicola Caporaso, Maurizio Paoloni, Caterina Pasquazzi, Giuliano Rizzardini, Giustino Parruti, Antonio Craxì, Sergio Babudieri, Massimo Andreoni, Mario Angelico, Carlo F. Perno, Francesca Ceccherini-Silberstein, for the HCV Italian Resistance Network Study Group: [.., P. Andreone, E. Loggi, G. Verucchi, ], Di Maio, Velia C., Cento, Valeria, Lenci, Ilaria, Aragri, Marianna, Rossi, Piera, Barbaliscia, Silvia, Melis, Michela, Verucchi, Gabriella, Magni, Carlo F., Teti, Elisabetta, Bertoli, Ada, Antonucci, Francescopaolo, Bellocchi, Maria C., Micheli, Valeria, Masetti, Chiara, Landonio, Simona, Francioso, Simona, Santopaolo, Francesco, Pellicelli, Adriano M., Calvaruso, Vincenza, Gianserra, Laura, Siciliano, Massimo, Romagnoli, Dante, Cozzolongo, Raffaele, Grieco, Antonio, Vecchiet, Jacopo, Morisco, Filomena, Merli, Manuela, Brancaccio, Giuseppina, Di Biagio, Antonio, Loggi, Elisabetta, Mastroianni, Claudio M., Pace Palitti, Valeria, Tarquini, Pierluigi, Puoti, Massimo, Taliani, Gloria, Sarmati, Loredana, Picciotto, Antonino, Vullo, Vincenzo, Caporaso, Nicola, Paoloni, Maurizio, Pasquazzi, Caterina, Rizzardini, Giuliano, Parruti, Giustino, Craxã¬, Antonio, Babudieri, Sergio, Andreoni, Massimo, Angelico, Mario, Perno, Carlo F., Ceccherini-Silberstein, Francesca, Nicolini, L. A., Magni, C. F., Russo, M. L., Gaeta, G. B., Di Marco, V., Di Maio, V. C., Sorbo, M. C., Mura, M. S., Di Maio, Velia C, Magni, Carlo F, Bellocchi, Maria C, Pellicelli, Adriano M, Mastroianni, Claudio M, Craxì, Antonio, Perno, Carlo F, and Ceccherini Silberstein, Francesca
- Subjects
Male ,0301 basic medicine ,hepatitis C virus ,Sustained Virologic Response ,Sofosbuvir ,Hepacivirus ,Drug Resistance ,resistance-associated substitutions ,Viral Nonstructural Proteins ,VARIANTS ,NS5A ,medicine.disease_cause ,Gastroenterology ,chemistry.chemical_compound ,0302 clinical medicine ,Recurrence ,INFECTION ,antiviral therapy ,Medicine ,hepatitis C viru ,Viral ,Treatment Failure ,Chronic ,direct-acting antivirals ,resistance test ,hepatology ,biology ,GENOTYPE 1 ,virus diseases ,Middle Aged ,Settore MED/07 - Microbiologia e Microbiologia Clinica ,Hepatitis C ,Italy ,Combination ,Interferon ,Drug Therapy, Combination ,Female ,030211 gastroenterology & hepatology ,Author Keywords:antiviral therapy ,RIBAVIRIN ,Sequence Analysis ,Human ,medicine.drug ,medicine.medical_specialty ,Daclatasvir ,Genotype ,Hepatitis C virus ,Antiviral Agents ,LONG-TERM PERSISTENCE ,DACLATASVIR ,03 medical and health sciences ,Drug Therapy ,Aged ,Drug Resistance, Viral ,Hepatitis C, Chronic ,Humans ,Interferons ,Mutation ,Ribavirin ,Sequence Analysis, DNA ,Hepatology ,TREATMENT-NAIVE ,Internal medicine ,Antiviral Agent ,resistance-associated substitution ,direct-acting antiviral ,Hepaciviru ,resistance test KeyWords Plus:HEPATITIS-C VIRUS ,business.industry ,Viral Nonstructural Protein ,DNA ,biology.organism_classification ,Clinical trial ,030104 developmental biology ,SOFOSBUVIR ,chemistry ,Sequence Analysi ,Immunology ,business - Abstract
Background & Aims: Despite the excellent efficacy of direct-acting antivirals (DAA) reported in clinical trials, virological failures can occur, often associated with the development of resistance-associated substitutions (RASs). This study aimed to characterize the presence of clinically relevant RASs to all classes in real-life DAA failures. Methods: Of the 200 virological failures that were analyzed in 197 DAA-treated patients, 89 with pegylated-interferon+ribavirin (PegIFN+RBV) and 111 without (HCV-1a/1b/1g/2/3/4=58/83/1/6/24/25; 56.8% treatment experienced; 65.5% cirrhotic) were observed. Sanger sequencing of NS3/NS5A/NS5B was performed by home-made protocols, at failure (N= 200) and whenever possible at baseline (N= 70). Results: The majority of the virological failures were relapsers (57.0%), 22.5% breakthroughs, 20.5% non-responders. RAS prevalence varied according to IFN/RBV use, DAA class, failure type and HCV genotype/subtype. It was 73.0% in IFN group vs 49.5% in IFN free, with the highest prevalence of NS5A-RASs (96.1%), compared to NS3-RASs (75.9% with IFN, 70.5% without) and NS5B-RASs (66.6% with IFN, 20.4% without, in sofosbuvir failures). In the IFN-free group, RASs were higher in breakthrough/non-responders than in relapsers (90.5% vs 40.0%, P= 2 DAA classes showed multiclass resistance, including 11/11 NS3+NS5A failures. Furthermore, 20.0% of patients had baseline-RASs, which were always confirmed at failure. Conclusions: In our failure setting, RAS prevalence was remarkably high in all genes, with a partial exception for NS5B, whose limited resistance is still higher than previously reported. This multiclass resistance advocates for HCV resistance testing at failure, in all three genes for the best second-line therapeutic tailoring.
- Published
- 2017
43. Novelties in evaluation and monitoring of human immunodeficiency virus-1 infection: Is standard virological suppression enough for measuring antiretroviral treatment success?
- Author
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Svicher, V., Marchetti, G., Ammassari, A., Ceccherini-Silberstein, F., Sarmati, L., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Bandera, A., Bonfanti, P., Bonora, S., Borderi, M., Calcagno, A., Capobianchi, M. R., Castagna, A., Castelli, F., Cauda, R., Celesia, B. M., Chirianni, A., Cicalini, S., Cingolani, A., Cinque, P., Clementi, M., Cossarizza, A., Monforte, A. D., D'Ettorre, G., De Luca, A., Di Biagio, A., Di Perri, G., Galli, M., Gianotti, N., Girardi, E., Guaraldi, G., Lazzarin, A., Lichtner, M., Lo Caputo, S., Madeddu, G., Maggi, P., Maggiolo, F., Marchetti, G. C., Mastroianni, C., Mussini, C., Nozza, S., Nunnari, G., Parisi, S., Parruti, G., Perno, C. F., Piconi, S., Carla, M., Ripamonti, D., Rusconi, S., Santoro, M., Sterrantino, G., Vullo, V., Zaccarelli, M., Zazzi, M., Svicher, V, Marchetti, G, Ammassari, A, Ceccherini-Silberstein, F, Sarmati, L, Andreoni, M, Angarano, G, Antinori, A, Antonelli, G, Bandera, A, Bonfanti, P, Bonora, S, Borderi, M, Calcagno, A, Capobianchi, M, Castagna, A, Castelli, F, Cauda, R, Celesia, B, Chirianni, A, Cicalini, S, Cingolani, A, Cinque, P, Clementi, M, Cossarizza, A, Monforte, A, D'Ettorre, G, De Luca, A, Di Biagio, A, Di Perri, G, Galli, M, Gianotti, N, Girardi, E, Guaraldi, G, Lazzarin, A, Lichtner, M, Lo Caputo, S, Madeddu, G, Maggi, P, Maggiolo, F, Mastroianni, C, Mussini, C, Nozza, S, Nunnari, G, Parisi, S, Parruti, G, Perno, C, Piconi, S, Carla, M, Ripamonti, D, Rusconi, S, Santoro, M, Sterrantino, G, Vullo, V, Zaccarelli, M, and Zazzi, M
- Subjects
Inflammation ,Genotypic resistance testing ,HIV-DNA ,Immune-activation ,Low level viremia ,Residual viremia - Abstract
The high potency and tolerability of the currently available antiretroviral drugs has modified HIV-1 infection from a life-threatening disease to a chronic illness. Nevertheless, some issues still remain open to optimize the management of HIV-1 infected patients in term of maintenance of virological suppression over time, identifying patients that could benefit from simplification therapy, and reducing co-mordibities driven by chronic inflammation. The availability of robust and affordable virological and immunological markers can help in solving these issues by providing information on the burden of HIV-1 reservoir in all the anatomical compartments in which the virus replicates as well as on persistent inflammation, immune activation and senescence despite successful virological suppression. In this light, this review is aimed at providing new insights (arising from a two-day Italian expert meeting hold in Rome in March 2016) in evaluation and monitoring of HIV-1 infection from a virological, immunological and clinical perspective. Particular attention has been focused on role of novel parameters (such as total HIV-1 DNA, residual viremia, and immunological markers) in optimizing treatment strategies, enhancing medical adherence, and individualizing monitoring.
- Published
- 2017
44. Immune-escape mutations and stop-codons in HBsAg develop in a large proportion of patients with chronic HBV infection exposed to anti-HBV drugs in Europe
- Author
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Colagrossi, L. Hermans, L.E. Salpini, R. Di Carlo, D. Pas, S.D. Alvarez, M. Ben-Ari, Z. Boland, G. Bruzzone, B. Coppola, N. Seguin-Devaux, C. Dyda, T. Garcia, F. Kaiser, R. Köse, S. Krarup, H. Lazarevic, I. Lunar, M.M. Maylin, S. Micheli, V. Mor, O. Paraschiv, S. Paraskevis, D. Poljak, M. Puchhammer-Stöckl, E. Simon, F. Stanojevic, M. Stene-Johansen, K. Tihic, N. Trimoulet, P. Verheyen, J. Vince, A. Lepej, S.Z. Weis, N. Yalcinkaya, T. Boucher, C.A.B. Wensing, A.M.J. Perno, C.F. Svicher, V. HEPVIR working group of the European Society for translational antiviral research (ESAR)
- Abstract
Background: HBsAg immune-escape mutations can favor HBV-transmission also in vaccinated individuals, promote immunosuppression-driven HBV-reactivation, and increase fitness of drug-resistant strains. Stop-codons can enhance HBV oncogenic-properties. Furthermore, as a consequence of the overlapping structure of HBV genome, some immune-escape mutations or stop-codons in HBsAg can derive from drug-resistance mutations in RT. This study is aimed at gaining insight in prevalence and characteristics of immune-associated escape mutations, and stop-codons in HBsAg in chronically HBV-infected patients experiencing nucleos(t)ide analogues (NA) in Europe. Methods: This study analyzed 828 chronically HBV-infected European patients exposed to ≥ 1 NA, with detectable HBV-DNA and with an available HBsAg-sequence. The immune-associated escape mutations and the NA-induced immune-escape mutations sI195M, sI196S, and sE164D (resulting from drug-resistance mutation rtM204 V, rtM204I, and rtV173L) were retrieved from literature and examined. Mutations were defined as an aminoacid substitution with respect to a genotype A or D reference sequence. Results: At least one immune-associated escape mutation was detected in 22.1% of patients with rising temporal-trend. By multivariable-analysis, genotype-D correlated with higher selection of ≥ 1 immune-associated escape mutation (OR[95%CI]:2.20[1.32-3.67], P = 0.002). In genotype-D, the presence of ≥ 1 immune-associated escape mutations was significantly higher in drug-exposed patients with drug-resistant strains than with wild-type virus (29.5% vs 20.3% P = 0.012). Result confirmed by analysing drug-naïve patients (29.5% vs 21.2%, P = 0.032). Strong correlation was observed between sP120T and rtM204I/V (P < 0.001), and their co-presence determined an increased HBV-DNA. At least one NA-induced immune-escape mutation occurred in 28.6% of patients, and their selection correlated with genotype-A (OR[95%CI]:2.03[1.32-3.10],P = 0.001). Finally, stop-codons are present in 8.4% of patients also at HBsAg-positions 172 and 182, described to enhance viral oncogenic-properties. Conclusions: Immune-escape mutations and stop-codons develop in a large fraction of NA-exposed patients from Europe. This may represent a potential threat for horizontal and vertical HBV transmission also to vaccinated persons, and fuel drug-resistance emergence. © 2018 The Author(s).
- Published
- 2018
45. A close monitoring of virological and immunological hepatitis B markers can improve the diagnosis of HBV reactivation in HBsAg-negative/anti-HBc-positive patients with oncohematological diseases
- Author
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Salpini, R., primary, Cerva, C., additional, Battisti, A., additional, Piermatteo, L., additional, Sacco, A., additional, Malagnino, V., additional, Arcese, W., additional, Cantonetti, M., additional, Ceccherini-Silberstein, F., additional, Perno, C.F., additional, Andreoni, M., additional, Sarmati, L., additional, and Svicher, V., additional
- Published
- 2019
- Full Text
- View/download PDF
46. The integration of Hepatitis B virus into human genome is a common event in the setting of HBeAg negative chronic infection: implications for an altered cell homeostasis and metabolism
- Author
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Salpini, R., primary, Carioti, L., additional, Battisti, A., additional, Colagrossi, L., additional, Piermatteo, L., additional, Surdo, M., additional, Cacciafesta, V., additional, Nuccitelli, A., additional, Hansi, N., additional, Ceccherini-Silberstein, F., additional, Perno, C.F., additional, Gill, Upkar S., additional, Kennedy, P.T.F., additional, and Svicher, V., additional
- Published
- 2019
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- View/download PDF
47. Key mutations in HBsAg C-terminus correlate with lower HBsAg levels in vivo, hinder HBsAg release in vitro and affect HBsAg structural stability in HBeAg-negative chronic HBV genotype D infection
- Author
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Piermatteo, L., primary, Battisti, A., additional, Carioti, L., additional, Anastasiou, O., additional, Gill, U.S., additional, Colagrossi, L., additional, Bertoli, A., additional, Aragri, M., additional, Iuvara, A., additional, Malagnino, V., additional, Cerva, C., additional, Lichtner, M., additional, Mastroianni, C.M., additional, De Sanctis, G.M., additional, Maurizio, P., additional, Marignani, M., additional, Pasquazzi, C., additional, Iapadre, N., additional, Mari, T., additional, Parruti, G., additional, Vecchiet, J., additional, Sarmati, L., additional, Andreoni, M., additional, Mario, A., additional, Grelli, S., additional, Kennedy, P., additional, Verheyen, J., additional, Silberstein, F. Ceccherini, additional, Perno, C.F., additional, Salpini, R., additional, and Svicher, V., additional
- Published
- 2019
- Full Text
- View/download PDF
48. Novelties in evaluation and monitoring of human immunodeficiency virus-1 infection: Is standard virological suppression enough for measuring antiretroviral treatment success?
- Author
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Svicher, V, Marchetti, G, Ammassari, A, CECCHERINI SILBERSTEIN, F, and Sarmati, L
- Subjects
Genotypic resistance testing ,HIV-DNA ,Immune-activation ,Inflammation ,Low level viremia ,Residual viremia ,Settore MED/17 - Malattie Infettive - Published
- 2017
49. Genetic signatures specifically clustered in immune active HBsAg regions correlate with immunosuppression-driven HBV reactivation: an extensive analysis of HBV genome
- Author
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Svicher, V, Salpini, R, Colagrossi, L, Battisti, A, Bellocchi, M, Alteri, C, Armenia, D, Di Santo, F, Carioti, L, Pollicita, M, Ricciardi, A, Lichtner, M, Mastroianni, C, Paoloni, M, Marignani, M, Maylin, S, Delaugerre, C, Morisco, F, Coppola, N, Marrone, A, Di Paolo, D, Sarrecchia, C, Sarmati, L, Andreoni, M, Angelico, M, Perno, C, Svicher, V, Salpini, R, Colagrossi, L, Battisti, A, Bellocchi, Mc, Alteri, C, Armenia, D, Di Santo, F, Carioti, L, Pollicita, M, Ricciardi, A, Lichtner, M, Mastroianni, C, Paoloni, M, Marignani, M, Maylin, S, Delaugerre, C, Morisco, F, Coppola, N, Marrone, A, Di Paolo, D, Sarrecchia, C, Sarmati, L, Andreoni, M, Angelico, M, and Perno, Cf
- Subjects
Settore MED/07 - Published
- 2015
50. A high genetic heterogeneity in HBsAg can affect immunogenicity in acute hepatitis B infection
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Aragri M, Coppola N, Alteri C, Battisti A, Sagnelli C, Pisaturo M, Bellocchi MC, Salpini R, Starace M, Armenia D, Carioti L, Pollicita M, Sagnelli E, Perno CF, Svicher V, Aragri, M, Coppola, N, Alteri, C, Battisti, A, Sagnelli, C, Pisaturo, M, Bellocchi, Mc, Salpini, R, Starace, M, Armenia, D, Carioti, L, Pollicita, M, Sagnelli, E, Perno, Cf, and Svicher, V
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Settore MED/07 - Published
- 2015
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