53 results on '"Sven Krengel"'
Search Results
2. Recommendations for routine MRI in children with congenital melanocytic nevi need revision Comment to Neale et al., J Am Acad Dermatol 87(5):1060-1068
- Author
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Sven Krengel, Hagen Ott, and Regula Waelchli
- Subjects
Dermatology - Published
- 2023
3. Development of an international core domain set for medium, large and giant congenital melanocytic naevi as a first step towards a core outcome set for clinical practice and research
- Author
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M S van Kessel, Sven Krengel, C. M. A. M. van der Horst, Heather C. Etchevers, W Oei, Albert Wolkerstorfer, A C Fledderus, Ph.I. Spuls, Suzanne G.M.A. Pasmans, I.J. Korfage, C.A.M. Eggen, Jan Kottner, Dermatology, APH - Methodology, APH - Quality of Care, Plastic, Reconstructive and Hand Surgery, ACS - Atherosclerosis & ischemic syndromes, AMS - Musculoskeletal Health, AMS - Rehabilitation & Development, Amsterdam Movement Sciences, ACS - Diabetes & metabolism, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Naevus International Patient Representative Working Group Leader, Dermatological group practice, Lübeck, Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Association du Naevus Géant Congénital, Naevus 2000 France-Europe and the Asociación Española de Nevus Gigante Congénito, Gall, Valérie, and Public Health
- Subjects
medicine.medical_specialty ,Consensus ,Delphi Technique ,Best practice ,MEDLINE ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Dermatology ,Outcome (game theory) ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Quality of life (healthcare) ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,melanoma ,Humans ,Medicine ,Patient Reported Outcome Measures ,Set (psychology) ,Nevus, Pigmented ,congenital nevi ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,business.industry ,outcome domains ,Core outcome set ,Focus group ,3. Good health ,Clinical trial ,Treatment Outcome ,clinical research ,Research Design ,Family medicine ,Quality of Life ,business ,Psychosocial ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Background: Medium, large and giant congenital melanocytic naevi (CMN) can impose a psychosocial burden on patients and families, and are associated with increased risk of developing melanoma or neurological symptoms. Lack of consensus on what outcomes to measure makes it difficult to advise patients and families about treatment and to set up best practice for CMN. Objectives: Fostering consensus among patient representatives and professionals, we aim to develop a core outcome set, defined as the minimum set of outcomes to measure and report in care and all clinical trials of a specific health condition. We focused on the ‘what to measure’ aspect, the so-called core domain set (CDS), following the COMET and CS-COUSIN guidelines. Methods: We conducted a systematic review to identify outcomes reported in the literature. Focus groups with patient representatives identified patient-reported outcomes. All these outcomes were classified into domains. Through e-Delphi surveys, 144 stakeholders from 27 countries iteratively rated the importance of domains and outcomes. An online consensus meeting attended by seven patient representatives and seven professionals finalized the CDS. Results: We reached consensus on six domains, four of which were applied to both care and research: ‘quality of life’, ‘neoplasms’, ‘nervous system’ and ‘anatomy of skin’. ‘Adverse events’ was specific to care and ‘pathology’ to research. Conclusions: We have developed a CDS for medium-to-giant CMN. Its application in reporting care and research of CMN will facilitate treatment comparisons. The next step will be to reach consensus on the specific outcomes for each of the domains and what instruments should be used to measure these domains and outcomes.
- Published
- 2021
4. Combinatorial effects of azacitidine and trametinib on NRAS ‐mutated melanoma
- Author
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Klara‐Maria Hanft, Ebrahem Hamed, Max Kaiser, Julia Würtemberger, Michaela Schneider, Torsten Pietsch, Ursula Feige, Frank Meiss, Sven Krengel, Charlotte Niemeyer, and Simone Hettmer
- Subjects
Male ,Mitogen-Activated Protein Kinase Kinases ,Nevus, Pigmented ,Skin Neoplasms ,Pyridones ,Membrane Proteins ,Pyrimidinones ,Hematology ,GTP Phosphohydrolases ,Oncology ,Child, Preschool ,Mutation ,Pediatrics, Perinatology and Child Health ,Azacitidine ,Meningeal Neoplasms ,Humans ,Melanoma ,Protein Kinase Inhibitors - Abstract
Congenital melanocytic nevus (CMN) syndrome represents a mosaic RASopathy, typically caused by postzygotic NRAS codon 61 mutations, which originate in ectodermal precursor cells and result in melanocyte deposits in the skin and central nervous system (CNS). Affected patients are prone to develop uniformly fatal melanomas in the skin and CNS. Here, we report the case of a 2.7-year-old male with CMN syndrome, diffuse leptomeningeal melanosis and CNS melanoma, who underwent experimental therapy with the DNA methyltransferase inhibitor azacitidine in combination with the mitogen-activated protein kinase (MEK) inhibitor trametinib with exceptional clinical and radiological response. Response to combination therapy appeared to be more durable than the treatment response observed in several other severely affected patients treated with trametinib for late-stage disease. Correspondingly, concomitant exposure to trametinib and azacitidine prevented development of trametinib resistance in NRAS-mutated human melanoma cells in vitro. Also, azacitidine was shown to inhibit growth and mitogen-activated protein kinase 1/2 (ERK1/2) phosphorylation of melanoma cells and act synergistically with trametinib to inhibit the growth of trametinib-resistant melanoma cells. These observations suggest that azacitidine enhances trametinib monotherapy and may represent a promising candidate drug for combination therapies to enhance the efficacy of MEK inhibitors in RAS-driven diseases.
- Published
- 2021
5. Multidisziplinäre Langzeitbetreuung und zeitgemäße chirurgische Therapie kongenitaler melanozytärer Nävi – Empfehlungen des Netzwerks Nävuschirurgie
- Author
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Sven Krengel, Sophie Böttcher-Haberzeth, Mechthild Sinnig, Otfrid Beck, Lisa Weibel, Hagen Ott, Karin Rothe, Susanne von der Heydt, Martin Theiler, Nicos Marathovouniotis, Regula Wälchli, Martina Hüging, Kralj Rok, Lutz Meyer, Wera Wendenburg, Helmut Breuninger, Ferdinand Kosch, Ingo Königs, Özlem Cangir, Clemens Schiestl, Beate Häberle, Kathrin Neuhaus, Miriam Fattouh, and Kornelia Böhler
- Subjects
Gynecology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,business.industry ,Medicine ,Dermatology ,business - Abstract
In den letzten Jahren hat sich unser Wissensstand zu kongenitalen melanozytaren Navi (KMN) stark erweitert. Die Autoren, eine interdisziplinare Gruppe von Arzten und Arztinnen mit umfangreicher Erfahrung in der Langzeitbetreuung und chirurgischen Therapie von Kindern und Erwachsenen mit KMN (Netzwerk Navuschirurgie, NNC), geben hier eine Stellungnahme zur Indikationsstellung, Planung und Durchfuhrung einer Behandlung unter diesen neuen Pramissen ab. Die Beratung der Eltern zum konservativen und/oder operativen Management von KMN muss im interdisziplinaren Austausch stattfinden und zu einer individuellen Planung des oft mehrstufigen Prozesses fuhren. Hierbei steht das asthetische Langzeitergebnis, nicht die Melanomprophylaxe, im Mittelpunkt. Bei keinem KMN gilt die Pramisse der „Entfernung um jeden Preis“. Mogliche therapiebedingte Nebenwirkungen (Hospitalisation, Wundheilungsstorungen etc.) mussen sorgfaltig mit den Erfolgsaussichten abgewogen werden. Unter diesen Gesichtspunkten ist insbesondere die Methode der Dermabrasion zu hinterfragen. Auf einer Tagung des NNC im September 2018 wurde eine konsentierte Wertung erarbeitet, nach der die Dermabrasion haufig zu Wundheilungsstorungen sowie zu kosmetisch ungunstiger oder hypertropher Narbenbildung fuhren kann und sehr haufig mit einer oft erst Jahre spater auftretenden Repigmentierung assoziiert ist. Zudem ist kein Nutzen im Sinne einer Melanomprophylaxe erkennbar. Daher sollte in Zukunft sorgfaltig auf die moglichen Risiken und den oft limitierten kosmetischen Nutzen der Dermabrasion hingewiesen werden.
- Published
- 2019
6. Multidisciplinary long‐term care and modern surgical treatment of congenital melanocytic nevi – recommendations by the CMN surgery network
- Author
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Mechthild Sinnig, Lutz Meyer, Beate Häberle, Otfrid Beck, Hagen Ott, Lisa Weibel, Miriam Fattouh, Kralj Rok, Kornelia Böhler, Martina Hüging, Wera Wendenburg, Clemens Schiestl, Helmut Breuninger, Kathrin Neuhaus, Sven Krengel, Martin Theiler, Ferdinand Kosch, Susanne von der Heydt, Karin Rothe, Ingo Königs, Regula Wälchli, Sophie Böttcher-Haberzeth, Nicos Marathovouniotis, Özlem Cangir, University of Zurich, and Krengel, Sven
- Subjects
Counseling ,Parents ,medicine.medical_specialty ,Skin Neoplasms ,Cicatrix, Hypertrophic ,Esthetics ,medicine.medical_treatment ,MEDLINE ,610 Medicine & health ,Context (language use) ,Dermatology ,Premises ,2708 Dermatology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Intervention (counseling) ,Humans ,Medicine ,10220 Clinic for Surgery ,Radiation treatment planning ,Adverse effect ,Melanoma ,Patient Care Team ,Nevus, Pigmented ,Wound Healing ,business.industry ,Dermabrasion ,Long-Term Care ,Surgery ,Long-term care ,Child, Preschool ,business ,Follow-Up Studies - Abstract
In recent years, our knowledge of congenital melanocytic nevi (CMN) has greatly expanded. This has led to a paradigm shift. The present article represents a commentary by an interdisciplinary group of physicians from German-speaking countries with extensive experience in long-term care and surgical treatment of children and adults with CMN (CMN surgery network, "Netzwerk Nävuschirurgie", NNC). The authors address aspects such as the indication for treatment as well as treatment planning and implementation under these new premises. Adequate counseling of parents on conservative and/or surgical management requires an interdisciplinary exchange among physicians and individualized planning of the intervention, which frequently involves a multi-stage procedure. Today, the long-term aesthetic outcome is at the center of any therapeutic endeavor, whereas melanoma prevention plays only a minor role. The premise of "removal at any cost" no longer holds. Potential treatment-related adverse effects (hospitalization, wound healing disorders, and others) must be carefully weighed against the prospects of a beneficial outcome. In this context, the use of dermabrasion in particular must be critically evaluated. At a meeting of the NNC in September 2018, its members agreed on a consensus-based position on dermabrasion, stating that the procedure frequently leads to impaired wound healing and cosmetically unfavorable or hypertrophic scarring. Moreover, dermabrasion is considered to be commonly associated with considerable repigmentation that usually occurs a number of years after the procedure. In addition, the NNC members saw no benefit in terms of melanoma prevention. In the future, physicians should therefore thoroughly caution about the potential risks and often limited cosmetic benefits of dermabrasion.
- Published
- 2019
7. Giant Congenital Melanocytic Nevus Accompanied by an Intracranial Arachnoid Cyst
- Author
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Sven Krengel, Sara Dusel, Alexander Enk, Christine Fink, Ferdinand Toberer, Nina Trenkler, and Holger A. Haenssle
- Subjects
Intracranial arachnoid ,Pathology ,medicine.medical_specialty ,business.industry ,giant congenital melanocytic nevus ,Articles ,Dermatology ,medicine.disease ,Oncology ,Arachnoid cyst ,Congenital melanocytic nevus ,RL1-803 ,arachnoid cyst ,Genetics ,medicine ,melanoma risk ,Cyst ,neurocutaneous melanocytosis ,business ,Molecular Biology ,Neurocutaneous melanocytosis - Published
- 2019
8. Melanozytäre Nävi im Kindesalter sind häufig
- Author
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Jürgen Kreusch and Sven Krengel
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,Medicine ,business - Abstract
Melanozytare Navi, also gutartige Proliferationen melanozytarer Zellen der Haut gehoren zu den haufigsten Diagnosen in einer Hautarztpraxis. Die Ahnlichkeit und Verwechslungsmoglichkeit mit einem Melanom ist fur viele Patienten der primare Konsultationsgrund („Ist das was Schlimmes?“). Besondere Aufmerksamkeit erfordern rasch entstandene Pigmentmale.
- Published
- 2017
9. Giant congenital melanocytic nevus with vascular malformation and epidermal cysts associated with a somatic activating mutation in BRAF
- Author
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Frédéric Fina, Helmuth Vorbringer, Christian Rose, Pauline Heux, Benjamin Schwarz, Nicolas Macagno, Birgit Kahle, Stéphane Zaffran, Sven Krengel, Irina Berger, Heather C. Etchevers, Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Catholique de Lille - Faculté de Médecine, Maïeutique, Sciences de la santé (FMMS), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Universitätsklinikum Schleswig-Holstein, Service d'Anatomo-Cyto-Pathologie et de NeuroPathologie [Hôpital de la Timone - APHM] (ACPNP), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE), Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), This study was supported by grants from Nevus Outreach, Inc., Asociación Española de afectados por Nevus Gigante Congénito, Association du Naevus Géant Congénital, Naevus 2000 France-Europe, Caring Matters Now, the RE(ACT) Community and aid-in-kind from the BeHeard Rare Disease Science Challenge., Université catholique de Lille - Faculté de médecine et de maïeutique (UCL FMM), Université catholique de Lille (UCL), and Etchevers, Heather
- Subjects
Male ,Proto-Oncogene Proteins B-raf ,0301 basic medicine ,Neuroblastoma RAS viral oncogene homolog ,Pathology ,medicine.medical_specialty ,melanocyte ,Vascular Malformations ,[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Epidermal Cyst ,Dermatology ,[SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics ,Biology ,General Biochemistry, Genetics and Molecular Biology ,BRAF ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Congenital melanocytic nevus ,medicine ,[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Humans ,Nevus ,HRAS ,skin and connective tissue diseases ,neoplasms ,Aged ,Nevus, Pigmented ,venous ,GNA11 ,integumentary system ,Vascular malformation ,congenital ,[SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology ,medicine.disease ,malformation ,030104 developmental biology ,Oncology ,[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics ,Mutation ,Venous malformation ,GNAQ ,[SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology ,nevus - Abstract
International audience; Giant congenital melanocytic nevi may be symptomatically isolated, or syndromic. Associations with capillary malformations are exceptional, and development of epidermal cysts has not been described. A 71-year old patient with a giant congenital melanocytic nevus of the lower back, buttocks and thighs was asymptomatic except for unexpected hemorrhage during partial surgical excision years before. Blunt trauma at age 64 initiated recurrent, severe pain under the nevus; multiple large epidermal cysts developed within it. Imaging and biopsy showed a large, non-pulsatile venous malformation intermingled with the deep nevus. A low-abundance, heterozygous BRAF c.1799T>A (p.V600E) mutation was present in both the gluteal and occipital “satellite” nevi; additional mutations in NRAS, GNAQ, GNA11, HRAS and PIK3CA were undetectable. This is the first demonstration of an identical BRAF mutation in multiple congenital nevi from the same individual, confirming genetic heterogeneity in giant nevi. This exceptional case indicates that constitutive activation of BRAF can be an underlying cause of unusual associations of giant nevi with vascular malformations, and that the latter may be included among the somatic RASopathies.
- Published
- 2018
10. Melanozytäre Nävi im Kindesalter sind häufig
- Author
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Sven Krengel
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,Medicine ,business - Abstract
Melanozytare Navi, also gutartige Proliferationen melanozytarer Zellen der Haut (auch: Muttermale, Leberflecken) gehoren zu den haufigsten Diagnosen in einer Hautarztpraxis. Die Ahnlichkeit und Verwechslungsmoglichkeit mit einem Melanom ist fur viele Patienten der primare Konsultationsgrund („Ist das was Schlimmes?“). Auch Padiater sind regelmasig mit dieser Fragestellung konfrontiert.
- Published
- 2015
11. Case Reports of Fatal or Metastasizing Melanoma in Children and Adolescents: A Systematic Analysis of the Literature
- Author
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Johanna Friesenhahn, Sven Krengel, Johanna Christina Neuhold, and Nina Gerdes
- Subjects
Male ,medicine.medical_specialty ,Skin Neoplasms ,Adolescent ,Dermatology ,Risk Factors ,Congenital melanocytic nevus ,medicine ,Humans ,In patient ,CNS Melanoma ,Child ,Childhood Melanoma ,Melanoma ,Neoplasm Staging ,Skin ,business.industry ,Age Factors ,Infant, Newborn ,Infant ,Mean age ,medicine.disease ,Surgery ,Child, Preschool ,Meta-analysis ,Pediatrics, Perinatology and Child Health ,Cutaneous melanoma ,Disease Progression ,Female ,business - Abstract
Childhood melanoma (ChM) is rare, with clinical and epidemiologic characteristics that differ from those of adult melanomas. The objective of the current study was to systematically identify and analyze case reports and case series of fatal and metastasizing ChM in the medical literature. ChM case reports with a fatal outcome or metastases were identified using a Medline search and subdivided into ChM developing in the absence of a congenital melanocytic nevus (ChM without CMN) and ChM associated with a CMN (ChM with CMN); 258 cases of ChM without CMN (206 cutaneous, 52 noncutaneous) were identified. In cutaneous ChM without CMN with a fatal outcome (n = 155), the mean age at diagnosis was 13.1 years (median 14 yrs). The mean Breslow index in this group was 8.5 mm for children ages 0 to 10 years and 3.7 mm for children ages 11 to 18 years. In ChM with CMN (n = 178; 112 cutaneous, 66 central nervous system [CNS]), the mean age at diagnosis was 5.8 years for cutaneous melanoma (median 3 yrs) and 5.5 years for CMN-associated CNS melanoma (median 3 yrs). The majority of CMN-associated cutaneous melanomas developed in small and giant CMN (vs medium and large); 53.9% of CNS melanomas developed in patients with multiple medium CMN. This study represents the largest and most complete synopsis of ChM case reports in the medical literature. Our analysis supports the view that cutaneous ChM without CMN (or associated with smaller CMN) differs in several important aspects from ChM associated with large or giant CMN.
- Published
- 2014
12. Kongenitale melanozytäre Nävi
- Author
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Sven Krengel, Clemens Schiestl, and Kathrin Neuhaus
- Subjects
030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis - Abstract
Grose angeborene Pigmentmale stellen Betroffene, Eltern und Arzte vor erhebliche Herausforderungen. Die Indikation zur chirurgischen Therapie wird heute nicht mehr in erster Linie in der Reduktion des Melanomrisikos gesehen, da dieses nicht so hoch ist wie fruher angenommen. Dagegen kann die Exzision eine erhebliche psychosoziale Verbesserung fur die von dieser stigmatisierenden Erkrankung Betroffenen bedeuten. Neben der seriellen Exzision wurde insbesondere die expandergestutzte Chirurgie in den letzten Jahren weiterentwickelt. Die Dermabrasion stellt wegen der regelmasig auftretenden Repigmentierungen nur ein Verfahren der zweiten Wahl dar, auch weil das Melanomrisiko hierdurch vermutlich nur geringfugig beeinflusst wird. Entscheidend fur die erreichbare Verbesserung sind eine sorgfaltige Planung des zeitlichen Ablaufs der Exzisionen unter Berucksichtigung der Belastungen fur Kind und Familie sowie die Durchfuhrung in einem erfahrenen Team aus Pflegepersonal, Psychologen und Arzten.
- Published
- 2017
13. Symmetric Plantar Melanocytic Nevi - Site-Specific Phenomenon or Pure Chance
- Author
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O Haase and Sven Krengel
- Subjects
Medial part ,medicine.medical_specialty ,Skin cancer screening ,integumentary system ,business.industry ,Mean age ,Trunk ,Dermatology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Plantar arch ,030220 oncology & carcinogenesis ,medicine.artery ,medicine ,Foot arch ,Random event ,skin and connective tissue diseases ,business ,neoplasms ,Prospective survey - Abstract
Background: Predilection sites of melanocytic nevi are highly variable; however, individual patterns (e.g. preponderant involvement of extremities, trunk, etc.) are often noticed. A symmetric appearance of nevi on both sides of the body has not been reported. Objective: Guided by an initial, incidental observation of symmetrically arranged plantar nevi, a prospective survey of palmoplantar nevi was carried out. Methods: Between January 2016, and January 2017, 886 patients underwent a full-body examination for skin cancer screening. Special attention was paid to the pigmentary characteristics of the palms and soles. Results: 12/886 patients exhibited symmetrically or nearly symmetrically distributed plantar nevi (1.35%; mean age 45 years; male-female ratio 1:1). The nevi were mostly situated on the medial part of the plantar arch (9/12 cases; 75%). Remarkably, not a single case of symmetric nevi was identified on the palms. Conclusion: Arguments in favour of the specifity of the finding are: 1) the lack of comparable findings in palmar skin, i.e., in a similar histological compartment and 2) the predominant localization in the foot arch. Symmetric plantar nevi appear to be a rare, but not a random event. Possible underlying causes of this phenomenon are discussed.
- Published
- 2017
14. Current Management Approaches for Congenital Melanocytic Nevi
- Author
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Sven Krengel and Ashfaq A. Marghoob
- Subjects
Risk ,Nevus, Pigmented ,medicine.medical_specialty ,Skin Neoplasms ,business.industry ,MEDLINE ,Dermatology ,Prognosis ,medicine.disease ,Malignancy ,Current management ,Intervention (counseling) ,Meningeal Neoplasms ,medicine ,Humans ,Melanocytes ,Nevus ,Misinformation ,Approaches of management ,business ,Melanoma ,Neurocutaneous melanocytosis - Abstract
Lack of information and misinformation abounds regarding the potential risks of malignancy, management approaches, benefits of surgical intervention, follow-up strategies, and overall prognosis for individuals with congenital melanocytic nevi (CMN). This review is intended to provide answers to questions that frequently arise shortly after the birth of individuals with CMN, especially of larger types.
- Published
- 2012
15. Meeting report from the 2011 international expert meeting on large congenital melanocytic nevi and neurocutaneous melanocytosis, Tübingen
- Author
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Mark Beckwith, Heather C. Etchevers, Sven Krengel, and Helmut Breuninger
- Subjects
medicine.medical_specialty ,Congenital diseases ,business.industry ,education ,Dermatology ,medicine.disease ,Patient advocacy ,humanities ,General Biochemistry, Genetics and Molecular Biology ,Oncology ,Family medicine ,Pediatric surgery ,Medicine ,Nevus ,Pediatric Neurology ,business ,Neurocutaneous melanocytosis - Abstract
An unconventional symposium on the subject of pathogenetic, clinical, and therapeutic aspects of large and giant congenital melanocytic nevi and neurocutaneous melanocytosis, was held at the University of Tubingen, Germany, on May 6-7, 2011. Exchanges were made between physicians from a wide range of clinical disciplines, including pathology, dermatology, plastic and pediatric surgery, neurosurgery, pediatric neurology and genetics; basic scientists in cell and developmental biology; psychologists; and an unprecedented gathering of international patient advocacy group representatives. This diversity of outlooks brought fresh perspectives to the discussions about current scientific and therapeutic advances in the field of these rare congenital diseases and their oncogenic associations. A roadmap for future actions sketched out promising therapeutic developments to follow and fostering of interdisciplinary collaboration among all the involved parties.
- Published
- 2011
16. Profiling the Response of Human Hair Follicles to Ultraviolet Radiation
- Author
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Wolfgang Funk, Tobias W. Fischer, Zhong-Fa Lu, Koji Sugawara, Mark Berneburg, Ralf Paus, York Kamenisch, Sven Krengel, and Sybille Hasse
- Subjects
Keratinocytes ,Programmed cell death ,Ultraviolet Rays ,Connective tissue ,Apoptosis ,Human skin ,DNA Fragmentation ,Dermatology ,Biology ,Biochemistry ,Inner root sheath ,Cell Degranulation ,Transforming Growth Factor beta2 ,chemistry.chemical_compound ,Organ Culture Techniques ,Adrenocorticotropic Hormone ,Lactate dehydrogenase ,medicine ,Humans ,Mast Cells ,Molecular Biology ,Melanins ,integumentary system ,Degranulation ,Cell Differentiation ,Dose-Response Relationship, Radiation ,Cell Biology ,Hair follicle ,Mitochondria ,Cell biology ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,alpha-MSH ,Immunology ,Female ,Keratinocyte ,Hair Follicle ,Cell Division ,Hair - Abstract
Excessive UVR ranks among the most harmful environmental influences on human skin. However, the direct impact of UVR on human skin appendages remains to be systematically investigated. Organ-cultured human anagen hair follicles in vitro were irradiated, and reduction of hair shaft elongation, premature catagen entry, and reduced hair matrix keratinocyte proliferation were observed upon irradiation with UVB (20/50 mJ cm(-2)). At 20 mJ cm(-2), apoptotic cell death prevailed (casp-3/p53 activation), whereas at 50 mJ cm(-2), necrotic cell death was predominant (lactate dehydrogenase increase). Mitochondrial common deletion and oxidatively damaged genomic DNA (8-OH-dG) was mainly observed at 20 mJ cm(-2). Follicular melanogenesis and ACTH immunoreactivity drastically declined, but alpha-melanocyte-stimulating hormone remained unchanged, whereas transforming growth factor-beta(2) expression shifted from the outer toward the inner root sheath. Both the number of Giemsa+ mast cells and the degree of mast-cell degranulation increased in the connective tissue sheath (CTS), and CD117 immunoreactivity of CTS cells and matrix keratinocytes was upregulated. Thus, UVR differentially modifies hair growth and cycle, promotes cell death, and induces complex regulatory events in human hair follicles in vitro. The leads from this human organ model, which is a living and human tissue interaction system under physiologically relevant in situ conditions, may encourage its use for general investigation of UV-induced effects as well as for testing possible agents for their UV-protective potency.
- Published
- 2009
17. Installation of a network for patients with congenital melanocytic nevi in German-speaking countries
- Author
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Helmut Breuninger, Volker Merl, Sven Krengel, Axel Hauschild, Peter Höger, and Henning Hamm
- Subjects
Nevus, Pigmented ,medicine.medical_specialty ,Skin Neoplasms ,Information Dissemination ,business.industry ,Subject (documents) ,Dermatology ,medicine.disease ,Community Networks ,Risk Assessment ,language.human_language ,German ,Patient Education as Topic ,Germany ,medicine ,language ,Humans ,Nevus ,Registries ,skin and connective tissue diseases ,business ,Melanoma - Abstract
Summary In 2005, an Internet-based network for the support of patients with congenital melanocytic nevi in German-speaking countries was started (http://www.naevus-netzwerk.de). Along with detailed information for patients and parents, the home-page includes a nevus registry which is based on an electronic questionnaire and which aims at providing data on the long-term course of nevi estimated to reach > 10 cm in largest diameter. In the past, congenital melanocytic nevi have been subject to various mythological interpretations (“Tierfellnavus”, lit.”animal coat nevus”;”Muttermal”). Today an increasing body of reliable scientific data allows a differentiated reflection of the risk of malignant transformation and has led to progress in the diagnostic and therapeutic management. Recent findings from the literature and considerations from scientific meetings are reviewed.
- Published
- 2008
18. Clinical course, treatment modalities, and quality of life in patients with congenital melanocytic nevi - data from the German CMN registry
- Author
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Detlef Zillikens, A Langenbruch, Sven Krengel, Maria Elisabeth Wramp, and Matthias Augustin
- Subjects
Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Skin Neoplasms ,Adolescent ,Dermatologic Surgical Procedures ,Dermatology ,Comorbidity ,Cohort Studies ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Age Distribution ,Quality of life ,Risk Factors ,Germany ,medicine ,Prevalence ,Humans ,Longitudinal Studies ,Registries ,Young adult ,Sex Distribution ,Prospective cohort study ,Child ,Nevus, Pigmented ,Stereotyping ,business.industry ,Dermatology Life Quality Index ,medicine.disease ,Interim analysis ,Treatment Outcome ,030220 oncology & carcinogenesis ,Austria ,Child, Preschool ,Health Care Surveys ,Cutaneous melanoma ,Quality of Life ,Female ,Nervous System Diseases ,business ,Switzerland ,Cohort study - Abstract
BACKGROUND Congenital melanocytic nevi (CMN) are associated with mental stress as well as medical risks for those affected. The German CMN registry was initiated in 2005. Herein, we present results from an interim analysis focusing on disease course, treatment modalities, and quality of life. PATIENTS AND METHODS One hundred patients enrolled in the registry between 2005 and 2012 were included in this prospective cohort study, and asked to participate in a follow-up survey. In addition, standardized questionnaires were used to collect data on quality of life (dermatology life quality index, DLQI) and perceived stigmatization (perceived stigmatization questionnaire, PSQ; social comfort questionnaire, SCQ). RESULTS Eighty-three percent of patients (or their parents) provided answers to the survey questions (mean patient age: 11.2 years, median: 6 years; mean follow-up: 4.4 years). Overall, four individuals were diagnosed with melanoma, including two pediatric cases with CNS melanoma, one adult with cutaneous melanoma, and one case which later turned out to be a proliferative nodule. Four children were diagnosed with neurocutaneous melanocytosis, three of whom exhibited neurological symptoms. Eighty-eight percent (73/83) of patients underwent surgery. Seventy-eight percent reported no or only minor impact of the CMN on quality of life. In general, perceived stigmatization and impairment of social well-being were also low. CONCLUSIONS Our results provide an overview of the situation of CMN patients in Germany, Austria, and Switzerland. Three percent of patients developed melanoma; 4 % showed CNS involvement.
- Published
- 2015
19. Temozolomide in Combination With Interferon-Alfa Versus Temozolomide Alone in Patients With Advanced Metastatic Melanoma: A Randomized, Phase III, Multicenter Study from the Dermatologic Cooperative Oncology Group
- Author
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Claus Garbe, Günther Sebastian, Ulrike Leiter, Reinhard Dummer, Jürgen C. Becker, Peter von den Driesch, Lutz Kretschmer, Roland Kaufmann, Dirk Schadendorf, Cornelia Mauch, Ruthild Linse, Wolfgang Tilgen, Sven Krengel, Konstanze Spieth, and Thomas Vogt
- Subjects
Adult ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Dacarbazine ,Alpha interferon ,Statistics, Nonparametric ,Internal medicine ,Multicenter trial ,Antineoplastic Combined Chemotherapy Protocols ,Temozolomide ,medicine ,Humans ,Prospective Studies ,Melanoma ,Survival rate ,Interferon alfa ,Aged ,Cancer staging ,Chi-Square Distribution ,business.industry ,Interferon-alpha ,Middle Aged ,Prognosis ,medicine.disease ,Survival Rate ,Treatment Outcome ,Female ,business ,medicine.drug - Abstract
Purpose Temozolomide (TMZ) has shown efficacy in metastatic melanoma equal to that of dacarbazine (DTIC), the standard chemotherapeutic agent for melanoma. As the combination with interferon-alfa (IFN-α) appears superior to single-agent DTIC regarding response rates, the purpose of this study was to compare TMZ alone and TMZ plus IFN-α in terms of objective response (OR), overall survival, and safety in a prospective, randomized, multicenter trial. Patients and Methods Two hundred ninety-four patients with untreated stage IV metastatic melanoma (American Joint Committee on Cancer staging system) were randomly assigned to receive either oral TMZ alone (200 mg/m2/day; days 1 through 5 every 28 days) or in combination with subcutaneous IFN-α (5 MU/m2; days 1, 3, and 5 every week). Results Two hundred eighty-two patients were eligible for an intent-to-treat analysis, 271 patients were treated per protocol. In the TMZ + IFN-α arm, 33 (24.1%) of 137 patients responded to therapy (partial or complete remission) whereas in the monotherapy arm, in 18 (13.4%) of 134 patients, a response was evident. Thus, the response rate was significantly higher in the combination arm (P = .036). Median survival time was 8.4 months for patients treated with TMZ (95% CI, 7.07 to 9.27) and 9.7 months for those treated with the combination (95% CI, 8.26 to 11.18; P = .16). Dose modifications and interval prolongations due to hematologic toxicity were significantly more frequent in the TMZ + IFN-α arm (P < .001). Conclusion In metastatic melanoma treatment with TMZ + IFN-α leads to a significantly superior OR rate compared to treatment with TMZ alone, which did not translate into prolonged survival in our study population.
- Published
- 2005
20. Nevogenesis-New Thoughts Regarding a Classical Problem
- Author
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Sven Krengel
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Skin Neoplasms ,Adolescent ,Melanocyte adhesion ,Dermatology ,Biology ,Pathology and Forensic Medicine ,Benign tumor ,medicine ,Ultraviolet light ,Humans ,Nevus ,Hamartoma ,Child ,skin and connective tissue diseases ,integumentary system ,Age Factors ,Infant ,General Medicine ,Melanocytic nevus ,medicine.disease ,Hair follicle ,Cell Transformation, Neoplastic ,medicine.anatomical_structure ,Child, Preschool ,Embryology ,Neuroscience - Abstract
The development of melanocytic nevi is a multifactorial and heterogeneous biologic process that involves prenatal and postnatal steps. As a consequence, there are two main perspectives to nevi: that of a hamartoma and that of a benign tumor. In this review, dermatopathological studies on congenital and acquired nevi, including studies on age-related and location-dependent changes, are analyzed. These studies have lead to different hypothetical concepts on the evolution of individual lesions. In the light of findings from experimental embryology and stem cell biology, we discuss the histogenesis of nevi with special reference to the temporospatial sequence of melanocyte-basement membrane interactions and hair follicle genesis. Regarding the mechanisms of postnatal nevus development, epidemiological studies demonstrate the importance of constitutional and environmental influences, especially ultraviolet light. Possible molecular pathways of solar nevogenesis involve ultraviolet-induced alterations of the cellular microenvironment (eg, changes in the expression of cytokines and melanocyte adhesion molecules). Recent results and future directions of clinical and experimental research are presented.
- Published
- 2005
21. Selective down-regulation of the alpha6-integrin subunit in melanocytes by UVB light
- Author
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Andreas Gebert, Michael Tronnier, Imke Stark, Sven Krengel, Bettina Knoppe, Peter Schlenke, Lutz Wünsch, Christian Geuchen, Jürgen Brinckmann, and Gabriele Scheel
- Subjects
Keratinocytes ,Time Factors ,Cell Survival ,Ultraviolet Rays ,media_common.quotation_subject ,Population ,Integrin ,Down-Regulation ,Apoptosis ,Dermatology ,Integrin alpha6 ,Melanocyte ,Biochemistry ,Flow cytometry ,Laminin ,Cell Adhesion ,medicine ,Humans ,Anoikis ,Annexin A5 ,education ,Internalization ,Molecular Biology ,Cells, Cultured ,media_common ,education.field_of_study ,Microscopy, Confocal ,integumentary system ,medicine.diagnostic_test ,biology ,urogenital system ,Melanoma ,Flow Cytometry ,medicine.disease ,Cell biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Microscopy, Fluorescence ,embryonic structures ,Immunology ,biology.protein ,Melanocytes ,Protein Binding - Abstract
In vivo, melanocytes bind to laminin (LM) molecules of the basement membrane (BM) via the integrins alpha3beta1 and alpha6beta1, and they adhere to neighbouring keratinocytes via E-cadherin. Only few studies have addressed the impact of ultraviolet (UV) light on the interaction of melanocytes with their microenvironment. In this report, we examined the influence of UVB irradiation on the expression of the most important melanocyte-adhesion molecules (E-, N-cadherin, alpha2-, alpha3-, alpha5-, alpha6-, alphaV-, beta1-, beta3-integrins and ICAM-1) in vitro by flow cytometry. We were able to demonstrate that the alpha6-integrin subunit is selectively and reversibly down-regulated by UVB in a dwzm 150ose-dependent manner. In comparison, keratinocytes lacked UVB-inducible alterations in the expression of alpha6-integrin. In the presence of LM-1, the UVB-induced down-regulation of alpha6-integrin in melanocytes was significantly reduced. Moreover, LM-1 increased the resistance of melanocytes to UVB-induced cell death, as measured by annexinV-binding analysis. This effect was reversed by preincubation with an alpha6-integrin-blocking antibody. By immunofluorescence, we could demonstrate that UVB leads to a dose-dependent internalization of alpha6-integrin, providing an obvious explanation for the down-regulation on the outer cell surface observed by flow cytometry. We suggest that adhesion to LM-1 through alpha6-integrin represents a protective mechanism for melanocytes to withstand UVB damage. Through alpha6-integrin internalization, sunburns might alter the interaction between melanocytes and the BM, resulting in apoptosis induced by loss of anchorage (anoikis). Repeated sunburns may then lead to the selection of a population of melanocytes which are capable of anchorage-independent survival, culminating in solar nevogenesis and melanoma development.
- Published
- 2005
22. Reactive angioendotheliomatosis: is it 'intravascular histiocytosis'?
- Author
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CH Mensing, Michael Tronnier, HH Wolff, and Sven Krengel
- Subjects
Pathology ,medicine.medical_specialty ,Skin Neoplasms ,business.industry ,Lymphoma, Non-Hodgkin ,Reactive angioendotheliomatosis ,Dermatology ,medicine.disease ,Lymphoma ,Histiocytosis ,Infectious Diseases ,medicine ,Blood Vessels ,Humans ,Female ,Endothelium, Vascular ,Vascular pathology ,Skin pathology ,business ,Aged ,Skin - Abstract
We report the case of a 68-year-old female with reactive angioendotheliomatosis (RAE). This case highlights the benign course of this condition and suggests that this entity might be an intravascular histiocytosis.
- Published
- 2005
23. Cadherin expression pattern in melanocytic tumors more likely depends on the melanocyte environment than on tumor cell progression
- Author
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Michael Tronnier, Sven Krengel, F. Grotelüschen, and S. Bartsch
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,Nevi and melanomas ,integumentary system ,Cell adhesion molecule ,Cadherin ,Melanoma ,Dermatology ,Biology ,Melanocyte ,medicine.disease ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Tumor progression ,Catenin ,medicine ,Nevus ,neoplasms - Abstract
Background: Adhesion molecules have been assigned an important role in melanocytic tumor progression. By the loss of E-cadherin, melanocytes might escape the control of neighbouring keratinocytes. Although in vitro data support this hypothesis, there are yet no conclusive immunohistochemical results on cadherin expression in melanocytic tumors. Objective: To gain detailed insight in the expression of cadherins and their cytoplasmic binding partners, the catenins, in various types of benign and malignant melanocytic neoplasms. Methods: Immunohistochemical analysis of the expression of E-, P-, and N-cadherin and α-, β-, and γ-catenin in compound and dermal nevi, Spitz nevi, blue nevi, ultraviolet B (UVB)-irradiated nevi, and malignant melanomas of various tumor thickness. Results: In both nevi and melanomas, E-cadherin expression in melanocytic cells decreased, following a gradient from junctional to deeper dermal localization. The pattern of E-cadherin expression was more heterogeneous in melanomas than in nevi. In some melanomas, E-cadherin was only weakly positive in the epidermal tumor cells. P-cadherin expression was similar to that of E-cadherin. N-cadherin expression in melanocytic lesions was a rare finding, however, a small percentage of melanomas showed expression in some cell nests. Some Spitz nevi exhibited strong N-cadherin immunoreactivity. Most melanocytic cells were α- and β-catenin-positive and γ-catenin-negative. UVB irradiation did not influence the expression of cadherins and catenins in melanocytic nevi in vivo. Conclusions: It is presumed that the gradual loss of E-cadherin expression represents a reaction of melanocytic cells to altered conditions in the dermal environment, e.g. lack of contact to keratinocytes, or new contact with dermal extracellular matrix molecules, respectively. Melanoma cells apparently are less dependent on these environmental factors and, therefore, show a more heterogeneous expression pattern. This might be of importance for the adaptation of the tumor cells to local requirements. However, in view of our results, a causative role of (loss of ) E-cadherin or (gain of ) N-cadherin for melanocytic tumor progression still remains to be proven.
- Published
- 2003
24. Fallberichte
- Author
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Werner Solbach, Helmut H. Wolff, Sven Krengel, Kristiane Kuhnt-Lenz, and Sebastian Fetscher
- Subjects
Pathology ,medicine.medical_specialty ,integumentary system ,medicine.diagnostic_test ,Sweet Syndrome ,Dermatology ,Vibrio vulnificus ,Disease ,Biology ,biology.organism_classification ,medicine.disease ,Microbiology ,Sepsis ,Baltic sea ,Vibrionaceae ,Vibrio Infections ,medicine ,Blood culture - Abstract
A 59-year-old female patient with a history of malignant lymphoma presented with symptoms of septicaemia. The skin of the extremities showed bullous, necrotizing plaques. Blood culture revealed Vibrio vulnificus as the causative organism. The infection was most likely acquired while swimming in the unusually warm Baltic Sea through inadvertent swallowing of sea water. The disease is rare in Europe. It is discussed in view of its typical clinical and histological picture.
- Published
- 2003
25. MMP-2, TIMP-2 and MT1-MMP are differentially expressed in lesional skin of melanocytic nevi and their expression is modulated by UVB-light
- Author
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M. Alexander, Jürgen Brinckmann, Sven Krengel, and Michael Tronnier
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,Epidermis (botany) ,Melanoma ,Junctional nevus ,Dermatology ,Melanocytic nevus ,Biology ,medicine.disease ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Dermis ,medicine ,Ultraviolet light ,Nevus ,skin and connective tissue diseases ,Keratinocyte - Abstract
Background: In malignant melanoma, recent studies have demonstrated an important role of matrix-metalloproteinase 2 (MMP-2), its co-activating enzyme membrane-type matrix-metalloproteinase 1 (MT1-MMP), and the endogenous inhibitor of MMP-2, tissue-inhibitor of matrix metalloproteinase 2 (TIMP-2). Melanocytic nevi are benign neoplasms of the melanocytic lineage, but may exhibit dysplastic features that can be difficult to distinguish from early stage melanoma. As shown in earlier studies, nevi show important morphological and phenotypical changes in response to ultraviolet light (UVB) irradiation. Objective: To clarify the role of MMP-2, TIMP-2 and MT1-MMP in UVB-irradiated vs. non-irradiated melanocytic nevi. Methods: Immunohistochemical comparison of the MMP-2, TIMP-2 and MT1-MMP expression pattern. Results: MMP-2 is expressed by lesional keratinocytes and its expression is up-regulated by UVB-irradiation. MMP-2 expression was not observed in melanocytic cells. TIMP-2, by contrast, is predominantly expressed by melanocytic nevus cells, and its expression is in part down-regulated by UVB-irradiation. MT1-MMP is expressed by basal keratinocytes and to a weaker extent by melanocytic nevus cells. Conclusions: MMP-2 expression by keratinocytes in nevi probably represents the result of activation of keratinocyte turnover in lesional epidermis. MMP-2 could play a role in the downward movement of junctional nevus cells into the dermis. The reduction of TIMP-2 expression in melanocytic cells by UV-light together with the enhanced expression of MMP-2 in the adjacent epidermis may promote basement membrane degradation. The expression pattern of MT1-MMP in close proximity to epithelial–mesenchymal interfaces underlines the synergistic role of MT1-MMP in this process.
- Published
- 2002
26. Practical application of the new classification scheme for congenital melanocytic nevi
- Author
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Judith O'Haver, Ashfaq A. Marghoob, Sven Krengel, Heather C. Etchevers, Harper N. Price, and Kellie S. Badger
- Subjects
Hypertrichosis ,Adult ,Male ,medicine.medical_specialty ,Skin Neoplasms ,Adolescent ,Classification scheme ,Physical examination ,Dermatology ,Melanosis ,Young Adult ,Surgical removal ,Surveys and Questionnaires ,medicine ,Nevus ,Humans ,In patient ,Registries ,Child ,Melanoma ,Physical Examination ,Nevus, Pigmented ,medicine.diagnostic_test ,business.industry ,Neurocutaneous Syndromes ,medicine.disease ,Surgery ,Adult size ,Pediatrics, Perinatology and Child Health ,Practice Guidelines as Topic ,Female ,business - Abstract
A new consensus-based classification of congenital melanocytic nevi (CMN) has recently been proposed. It includes categories for projected adult size (PAS) and location, satellite nevi counts, and morphologic characteristics (color heterogeneity, rugosity, nodularity, and hypertrichosis). The objective of the current study was to test the applicability of the new categorization scheme and to correlate classification outcome with the patient's history of melanoma and neurocutaneous melanocytosis (NCM). Children and adults with CMN attending a patient conference in Dallas, Texas, in 2012 were invited to participate in the study. Anamnestical data were collected using a standardized questionnaire. Two dermatologists performed clinical examinations. Of 45 patients enrolled, 33 had a giant CMN (G1 [>40 cm PAS], n = 13; G2 [>60 cm PAS], n = 20), 12 had an NCM (5 symptomatic, 7 asymptomatic), and 1 had a history of melanoma. CMN size was positively correlated with NCM (p < 0.05). The classification system allowed an easy and detailed phenotypic characterization of each individual CMN. CMN size and morphology were difficult to assess in patients after surgical removal, and the number of satellite nevi at birth or during infancy was not always known. Our report provides practical aids for the application of the newly proposed CMN classification. Prospective evaluation of accurately classified patients in CMN registries will reveal the predictive value of the scheme. The small study sample limits meaningful conclusions regarding the correlation between CMN parameters and the risk of NCM and melanoma.
- Published
- 2014
27. Livedo racemosa: Ungewöhnliche Spätmanifestation einer Borreliose?
- Author
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S. Goerdt, M Baumann, Michael Arnold, Sven Krengel, C. E. Orfanos, and Beate Tebbe
- Subjects
Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Borrelia Burgdorferi Infection ,Dermatology ,Livedo racemosa ,medicine.disease ,Lyme disease ,Lymphadenosis Benigna Cutis ,Female patient ,Biopsy ,medicine ,Erythema chronicum migrans ,medicine.symptom ,skin and connective tissue diseases ,business ,Acrodermatitis chronica atrophicans - Abstract
Classic variants of cutaneous borreliosis are erythema chronicum migrans (ECM), lymphadenosis benigna cutis (LBC) and acrodermatitis chronica atrophicans (ACA). Other dermatoses have been reported in the literature as possibly linked to borreliosis. A 59-year old female patient was seen in the late phases of cutaneous borreliosis with histologically confirmed ACA. In addition, prominent livedo racemosa was seen on the legs, also showing tissue changes similar to those of ACA. Borrelia burgdorferi infection was serologically confirmed by the presence of anti-IgM and anti-IgG antibodies. The clinical spectrum of late cutaneous borreliosis should be enlarged to include livedo racemosa.
- Published
- 2000
28. Radiogene Akne
- Author
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S. Goerdt, Lutz-Uwe Wölfer, Beate Tebbe, Christos C. Zouboulis, Nestoris S, Claus-Detlev Klemke, and Sven Krengel
- Subjects
medicine.medical_specialty ,business.industry ,Radiation induced ,Dermatology ,Carbamazepine ,medicine.disease ,Oropharyngeal Carcinoma ,High doses ,Medicine ,Photon beam ,business ,Acne ,medicine.drug - Abstract
Radiation-induced acne is a rare, clinically and pathogenetically ill-defined acneiform dermatosis with special features that may occur in irradiated skin areas especially after high doses of deeply penetrating radiation. We report on a patient with an oropharyngeal carcinoma who developed severe radiation-induced acne including comedones and cysts as well as few inflammatory papules and pustules in a skin area irradiated with up to 63 gray of a 6 MeV photon beam. Acnegenic drugs may precipitate the disease; our patient was on longterm therapy with carbamazepine whose acnegenic potency is less well documented than that of testosterone or glucocorticoids. Treatment of radiation-induced acne is comedolytic; topical retinoids are especially valuable.
- Published
- 2000
29. Unilateral angiolymphoid hyperplasia with eosinophilia involving the left arm and hand
- Author
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Constantin E. Orfanos, J. Spröder, Sarah E. Coupland, Michael Arnold, Sven Krengel, S. Goerdt, Christoph C. Geilen, and Edgar Dippel
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Histology ,Brachial Artery ,Angiolymphoid hyperplasia ,Dermatology ,Disease ,Pathology and Forensic Medicine ,Diagnosis, Differential ,Fingers ,Biomarkers, Tumor ,medicine ,Humans ,Eosinophilia ,Angiolymphoid hyperplasia with eosinophilia ,Inflammatory lesion ,Vascular disease ,business.industry ,Angiolymphoid Hyperplasia with Eosinophilia ,Hand ,medicine.disease ,Immunohistochemistry ,Benign Vascular Neoplasm ,Arm ,Female ,medicine.symptom ,Differential diagnosis ,business - Abstract
A case report of recurrent angiolymphoid hyperplasia with eosinophilia (ALHE) in an otherwise healthy 20-year-old female with manifestation of the disease limited to the left arm and hand is presented together with brief evaluation of the literature as well as the features distinguishing ALHE and Kimura's disease. Immunohistochemical investigations support the hypothesis that ALHE represents a reactive inflammatory lesion rather than a benign vascular neoplasm. A viral cause of ALHE (e.g., HHV8 or Epstein-Barr virus (EBV)) could not be demonstrated. The recurrent nature of the disease is shown by this case, which also demonstrates the need for frequent medical and surgical management.
- Published
- 1999
30. Hepatitis-C-Virus-assoziierte Dermatosen
- Author
-
Beate Tebbe, Marina Stöffler-Meilicke, Constantin E. Orfanos, Sven Krengel, and Sergij Goerdt
- Subjects
Allergy ,integumentary system ,business.industry ,Hepatitis C virus ,Dermatology ,Necrolytic migratory erythema ,medicine.disease ,medicine.disease_cause ,Liver disease ,Psoriasis ,Immunology ,medicine ,Porphyria cutanea tarda ,Viral disease ,skin and connective tissue diseases ,business ,Cryoglobulinemic vasculitis - Abstract
Acute infection with hepatitis C virus (HCV) is often clinically inapparent, but may affect several organ systems in its chronic course. In dermatology, common diseases such as lichen planus, cryoglobulinemic vasculitis and porphyria cutanea tarda have been described in association with HCV infection. A number of other dermatologic disorders, e.g., psoriasis, chronic urticaria, chronic pruritus, pseudo-kaposi sarcoma, necrolytic migratory erythema and Behcet disease, have been associated in case reports with HCV-induced liver disease. In this study we summarize the recent literature reports, present three patients observed by our group and update the topic.
- Published
- 1999
31. Kaposiforme, HHV-8-negative Akroangiodermatitis bei chronisch-venöser Insuffizienz
- Author
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S. Goerdt, P. Schnitzler, Katharina Krüger, Beate Tebbe, C. E. Orfanos, Ulrike Blume-Peytavi, Sven Krengel, and M. Geiss
- Subjects
Pathology ,medicine.medical_specialty ,Chronic venous insufficiency ,business.industry ,Dermatology ,Both lower legs ,medicine.disease ,medicine.anatomical_structure ,Dermis ,Female patient ,medicine ,Immunohistochemistry ,Sarcoma ,Acroangiodermatitis ,Venous disease ,business - Abstract
A 76-year-old female patient developed severe manifestations of a kaposi-like acroangiodermatitis (so-called Mali's disease) due to chronic venous insufficiency of the lower extremities. The patient presented with large areas of confluent, violaceous or brown-black papules on both lower legs. Histologically, proliferation of thick-walled capillaries was seen in the upper dermis consisting of fully differentiated endothelial cells, as shown by immunohistochemistry. In contrast to true Kaposi's sarcoma, human-herpes-virus-8 DNA could not be detected by polymerase-chain-reaction in this condition. We review the diagnostic criteria used to distinguish between acroangiodermatitis, also called pseudo-Kaposi's sarcoma, and the true Kaposi's sarcoma.
- Published
- 1999
32. Erworbene reaktiv perforierende Dermatose
- Author
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Katharina Krüger, Sergij Goerdt, Constantin E. Orfanos, Beate Tebbe, and Sven Krengel
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,medicine ,Dermatology ,business - Abstract
Die perforierenden Dermatosen bilden eine heterogene Gruppe von Erkrankungen, die durch transepidermale Eliminationsphanomene gekennzeichnet sind. Abzugrenzen sind die primar perforierenden Dermatosen von solchen perforierenden Dermatosen, bei denen Perforation oder Elimination sekundar und als seltenes Teilsymptom bei bestehenden Dermatosen anderer Provenienz auftreten konnen. Zu den primar perforierenden Dermatosen zahlen dabei als Prototypen die Hyperkeratosis follicularis et parafollicularis in cutem penetrans (M. Kyrle), die Elastosis perforans serpiginosa und die perforierende Follikulitis. Die erworbene reaktiv perforierende Dermatose (syn. erworbene reaktiv perforierende Kollagenose) stellt zusammen mit der hereditaren, reaktiv perforierenden Kollagenose eine weitere Variante der primar perforierenden Dermatosen dar. Wir stellen hier eine 84jahrige und eine 96jahrige Patientin mit einer erworbenen reaktiv perforierenden Dermatose vor. Bei beiden Patientinnen lagen eine diabetogene und eine hyperurikamische Stoffwechsellage vor. Eine Therapie mit Allopurinol (100 mg/d) fuhrte bereits nach kurzer Dauer (1 bis 2 Wochen) zu einer weitgehenden Abheilung der disseminierten Lasionen. Uber einen Nachbeobachtungszeitraum von jeweils 6 Monaten befanden sich beide Patientinnen in Vollremission. Diese Beobachtungen belegen erneut die Existenz und Massivitat der Erkrankung und zeigen, das Allopurinol neben der bekannten urikostatischen Wirkung moglicherweise in der Haut immunologische oder differenzierungsmodulierende Effekte vermittelt.
- Published
- 1999
33. Phakomatosis pigmentovascularis type IIa
- Author
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Sergij Goerdt, Sven Krengel, Ulrike Blume-Peytavi, Christine Pfrommer, and Nathalie Mandt
- Subjects
Male ,Nevus, Pigmented ,Pathology ,medicine.medical_specialty ,Skin Neoplasms ,business.industry ,Port-Wine Stain ,Remission, Spontaneous ,Dermatology ,medicine.disease ,Phakomatosis pigmentovascularis ,Child, Preschool ,Mutation ,medicine ,Humans ,Child ,business ,Follow-Up Studies ,Skin - Published
- 1999
34. Das Vena-cava-superior-Syndrom
- Author
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Beate Tebbe, Susanne Tenorio, Sergij Goerdt, Christoph C. Geilen, Sven Krengel, and Constantin E. Orfanos
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,Superior vena cava ,Medicine ,Dermatology ,business - Abstract
Das Vena-cava-superior-Syndrom ist klinischer Ausdruck einer venosen Stauung im Ableitungsgebiet der oberen Hohlvene. Als Manifestation dieses relativ seltenen Symptomenkomplexes steht eine haufig akut entstehende Gesichtsschwellung im Vordergrund, die den Patienten zunachst zum Dermatologen fuhrt. Weitere klinische Charakteristika des Vena-cava-superior-Syndroms sind Rotung bzw. Zyanose des Gesichts, Stauung der Halsvenen und, bei langerem Bestand, Entwicklung von Kollateralvenen im Brustwandbereich. Die haufigste Ursache des Vena-cava-superior-Syndroms ist heute ein ausgepragter raumfordernder Prozes im Mediastinum, der langsam-progredient oder auch akut-subakut zur oberen Einflus-Stauung fuhrt. Die Erkrankung kann auch einen hochdramatischen, z.T. lebensbedrohlichen Verlauf nehmen, der intensiv-diagnostische bzw. intensiv-therapeutische Masnahmen erfordert. In der vorliegenden Arbeit werden drei eindrucksvolle Falle eines Vena-cava-superior-Syndrom vorgestellt, die a) durch ein Bronchialkarzinom, b) eine bihilare Sarkoidose sowie c) durch ein im Mediastinalraum metastasierendes malignes Melanom bedingt waren. Dabei wird das Krankheitsbild unter besonderer Berucksichtigung der notwendigen diagnostischen und therapeutischen Masnahmen anhand des bisherigen Schrifttums zusammenfassend dargestellt.
- Published
- 1997
35. Differentialdiagnostik bei Lidschwellungen
- Author
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K. F. Kölmel and Sven Krengel
- Subjects
Gynecology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,business.industry ,Diagnostico diferencial ,030221 ophthalmology & optometry ,medicine ,Dermatology ,business ,3. Good health - Abstract
An den Augenlidern manifestieren sich unterschiedlichste Erkrankungen. Ein haufiges Symptom dieser Region ist die Schwellung, der wiederum oft ein Odem zugrundeliegt. Neben einer Beschreibung der pathophysiologischen und anatomischen Grundlagen soll im vorliegenden Text vor allem eine praktische Anleitung – unter anderem mit Flusdiagrammen – zum differentialdiagnostischen Vorgehen bei Patienten mit Lidschwellung gegeben werden. Hierbei erweist sich die subjektive Symptomatik (Schmerzen, Juckreiz) als sinnvolles Orientierungskriterium. Die Erkrankungen werden, nach Inzidenz gereiht, in tabellarischer Form charakterisiert. Besonderes Augenmerk gilt den allergischen Ursachen fur Lidschwellungen sowie dem Zusammenhang zwischen Lidschwellung und atrophischen Lidveranderungen.
- Published
- 1996
36. New recommendations for the categorization of cutaneous features of congenital melanocytic nevi
- Author
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Ashfaq A. Marghoob, Sven Krengel, Stephen W. Dusza, Alon Scope, and Reinhard Vonthein
- Subjects
Adult ,Observer Variation ,medicine.medical_specialty ,Nevus, Pigmented ,Skin Neoplasms ,business.industry ,Adverse outcomes ,Maximal diameter ,Infant ,First year of life ,Dermatology ,medicine.disease ,Prognosis ,Small Congenital Melanocytic Nevus ,Categorization ,Congenital melanocytic nevus ,medicine ,Nevus ,Humans ,business ,Kappa - Abstract
Background The diameter of congenital melanocytic nevi (CMN) has served as the lone criterion for determining risks of adverse outcomes such as melanoma. A standardized description of additional morphologic features is needed. Objective We sought to develop a consensus-based standardized categorization of cutaneous features of CMN and to test agreement among experts on the proposed scheme. Methods An interdisciplinary group of experts in the field of CMN was surveyed using a detailed questionnaire. Applicability of the expert consensus-based scheme was tested for interobserver agreement. Results The principal variable of the consensus-based categorization is CMN size, based on maximal diameter the CMN is projected to attain by adulthood. CMN size categories include: small ( 10-20 cm); large (L1: >20-30 cm, L2: >30-40 cm); and giant (G1: >40-60 cm, G2: >60 cm). In addition, number of satellite nevi in the first year of life is categorized into none, 1 to 20, more than 20 to 50, and more than 50 satellites. Additional descriptors of CMN include anatomic localization, color heterogeneity, surface rugousity and presence of hypertrichosis (described as none, moderate, marked), and presence of dermal or subcutaneous nodules (none, scattered, extensive). Assessment of consistency among 3 experts showed moderate to excellent interobserver agreement for categorization of the clinical descriptors (kappa values 0.54-0.93). Limitations Applicability of the proposed scheme was tested in a virtual setting and only among experts. Conclusion The proposed categorization scheme for CMN was agreed upon by experts and showed good interobserver agreement. Such standardized reporting of patients with CMN facilitates the development of an international clinical database for the study of large and giant CMN.
- Published
- 2012
37. Clinical and epidemiological aspects of subtypes of melanocytic nevi (Flat nevi, Miescher nevi, Unna nevi)
- Author
-
Constanze Witt and Sven Krengel
- Subjects
Adult ,Male ,medicine.medical_specialty ,Skin Neoplasms ,Keratosis ,Adolescent ,Dermatology ,Neoplasms, Multiple Primary ,Young Adult ,Epidemiology ,medicine ,Nevus ,Humans ,Age of Onset ,skin and connective tissue diseases ,Caucasian population ,Head and neck ,Child ,Keratosis, Seborrheic ,neoplasms ,Aged ,Aged, 80 and over ,Lentigo ,Nevus, Pigmented ,integumentary system ,business.industry ,Melanoma ,General Medicine ,Melanocytic nevus ,Middle Aged ,medicine.disease ,Child, Preschool ,Sunlight ,Female ,Age of onset ,Facial Neoplasms ,business - Abstract
The term "melanocytic nevus" comprises a group of clinically and pathologically distinct subtypes. In this prospective clinical study we evaluated the frequency, localization, and age and gender distribution of flat nevi (FN), Miescher nevi (MN), and Unna nevi (UN) in a caucasian population. Nevi were counted in 400 patients, of which 47 had a history of melanoma. Additionally, the patients answered to a detailed UV questionnaire. Flat nevi represented the most common type of melanocytic nevi, with a peak in the 3rd decade of life. They were mostly found on the extremities and on the trunk. Miescher nevi were most common in the 6th decade and were predominantly found in the head and neck region. Unna nevi showed a maximum in the 8th decade and they were mainly situated on the trunk. The counts of all three nevus subtypes were elevated in the melanoma group. Our results confirm that FN, MN, and UN represent melanocytic nevi with distinctive morphological and clinical characteristics. The role of sunlight seems to be more prominent in the pathogenesis of FN. The precise description of FN, MN, and UN may serve as a base for a pathogenetic distinction of subtypes of melanocytic nevi in the future.
- Published
- 2010
38. Melanocytic Tumors
- Author
-
Jivko A. Kamarashev, Leo Schärer, Marie C. Zipser, Lauren L. Lockwood, Reinhard Dummer, and Sven Krengel
- Published
- 2010
39. Aufbau eines deutschsprachigen Netzwerks für Patienten mit kongenitalen melanozytären Nävi
- Author
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Sven Krengel, Henning Hamm, Axel Hauschild, Helmut Breuninger, Volker Merl, and Peter Höger
- Subjects
Dermatology - Published
- 2008
40. Timing and targeting of cell-based VEGF165 gene expression in ischemic tissue
- Author
-
Sven Krengel, Stefan Krüger, Til Aach, Thilo Wedel, Thomas Hellwig-Bürgel, Guido Middeler, Timo Spanholtz, C. Niedworok, Oliver J. Müller, A. Maichle, Wolfgang-Michael Franz, Werner Lindenmaier, Augustinus Bader, Beate M. Stoeckelhuber, and Hans-Guenther Machens
- Subjects
Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,Time Factors ,Endothelium ,Angiogenesis ,Ischemia ,Neovascularization, Physiologic ,Transfection ,Surgical Flaps ,Adenoviridae ,Neovascularization ,Rats, Sprague-Dawley ,medicine ,Animals ,Therapeutic angiogenesis ,Cell Proliferation ,business.industry ,Genetic transfer ,Gene Transfer Techniques ,Genetic Therapy ,Fibroblasts ,medicine.disease ,Rats ,Endothelial stem cell ,medicine.anatomical_structure ,Gene Expression Regulation ,Microangiography ,Models, Animal ,Surgery ,Female ,Endothelium, Vascular ,medicine.symptom ,business - Abstract
Background Therapeutic angiogenesis has become a key technology in experimental and clinical medicine. Only few data are available on the effects of timing and targeting of therapeutic proteins after cell-based gene transfer. This work investigates such effects after temporary expression of vascular endothelial growth factor 165 (VEGF165), the most commonly used angiogenic protein for therapeutic purposes. Methods We established a cell-based gene-transfer model using fibroblasts to temporarily produce VEGF165. Cells were implanted into 40 rats. Protein expression and angiogenic effects were measured by PCR, immunohistology, and microangiography. To determine an improvement for survival of ischemically challenged tissue, cells were implanted in an ischemic flap model at different locations and time points. Results After implantation of modified cells, a temporary increase was found in the target tissue for VEGF165, endothelial cell counts, and capillary network formations. Four wk later, histological alterations in the target tissue area were not different from controls. Implantation of modified cells into flap plus wound margin 1 wk before surgery showed significant improvement of tissue survival demonstrated by planimetric measurements and blood vessels counting in the target tissue. Conclusion In our model, temporary expression of VEGF165 induces therapeutically relevant angiogenesis and improves blood supply only if applied 1 wk before ischemia. It is essential to include the surrounding area for induction of angiogenesis in this model. In contrast, the angiogenic effects are not effective in the target area and its surrounding tissue, if therapeutic gene expression is started during onset of ischemia or 2 wk before ischemia in this model.
- Published
- 2007
41. [The role of eotaxins in bronchial asthma and nasal polyposis]
- Author
-
Luis M, Terán, Yadira, Ledesma-Soto, Sven, Krengel, and Diana, Lezcano-Meza
- Subjects
Chemokine CCL11 ,Nasal Polyps ,Chemokine CCL26 ,Chemokines, CC ,Chemokine CCL24 ,Humans ,Asthma - Abstract
Over the last few years, three specific eosinophil activating peptides, eotaxin-1, -2 and -3, members of the chemokine family have been identified. These cytokines exert a number of functions on eosinophils including chemotaxis, transendothelial migration and induction of the release of reactive oxygen species. Eosinophils are considered to play an important role in allergic disease by causing tissue damage through the release of toxic proteases, lipid mediators, cytokines and oxygen free radicals. This article reviews the role of eotaxins in asthma and nasal polyps. Discussion focuses on therapeutic guidelines, particularly as it has been shown that CCR3, the major chemokine receptor in eosinophils, serves as a eotaxin receptor.
- Published
- 2006
42. [Vibrio vulnificus sepsis]
- Author
-
Sven, Krengel, Kristiane, Kuhnt-Lenz, Sebastian, Fetscher, Werner, Solbach, and Helmut H, Wolff
- Subjects
Lymphoma, B-Cell ,Time Factors ,Remission Induction ,Cefotaxime ,Middle Aged ,Sweet Syndrome ,Anti-Bacterial Agents ,Treatment Outcome ,Sepsis ,Vibrio Infections ,Injections, Intravenous ,Humans ,Female ,Vibrio vulnificus ,Swimming ,Skin - Abstract
A 59-year-old female patient with a history of malignant lymphoma presented with symptoms of septicaemia. The skin of the extremities showed bullous, necrotizing plaques. Blood culture revealed Vibrio vulnificus as the causative organism. The infection was most likely acquired while swimming in the unusually warm Baltic Sea through inadvertent swallowing of sea water. The disease is rare in Europe. It is discussed in view of its typical clinical and histological picture.
- Published
- 2005
43. Absence of autoantibodies against correctly folded recombinant fibrillin-1 protein in systemic sclerosis patients
- Author
-
Jürgen, Brinckmann, Nico, Hunzelmann, Ehab, El-Hallous, Thomas, Krieg, Lynn Y, Sakai, Sven, Krengel, and Dieter P, Reinhardt
- Subjects
Male ,Extracellular Matrix Proteins ,Protein Folding ,Scleroderma, Systemic ,integumentary system ,Fibrillin-1 ,Microfilament Proteins ,Enzyme-Linked Immunosorbent Assay ,macromolecular substances ,Middle Aged ,Fibrillins ,Recombinant Proteins ,Microscopy, Electron, Transmission ,Humans ,Female ,skin and connective tissue diseases ,Protein Structure, Quaternary ,Cells, Cultured ,Autoantibodies ,Research Article - Abstract
Autoantibodies against short recombinant fragments of fibrillin-1 produced in bacterial expression systems have been found in tight-skin mouse, systemic sclerosis, mixed connective tissue disease, and primary pulmonary hypertension syndrome. In patients with scleroderma, the frequency of anti-fibrillin-1 antibodies was 42% in Caucasians. Until now it has been unclear whether this immune response has a primary function in disease pathogenesis or is a secondary phenomenon. In the present study we analyzed the frequency of autoantibodies against two overlapping recombinant polypeptides spanning the N-terminal and C-terminal halves of human fibrillin-1, which were produced in human embryonic kidney (HEK-293) cells. Correct three-dimensional structures of the recombinant fibrillin-1 polypeptides were shown by electron microscopy and immunoreactivity with antibodies. Screening of fibrillin-1 antibodies was performed in 41 sera from systemic sclerosis patients and in 44 healthy controls with a Caucasian background. Microtiter plates were coated with the recombinant polypeptides of fibrillin-1 and incubated with 1:100 diluted sera. Positive binding was defined as being more than 2 SD above the mean of the control group. ELISAs showed that none of the sera of patients with systemic sclerosis contained autoantibodies against the N-terminal or C-terminal recombinant fibrillin-1 polypeptide. The data show the absence of autoantibodies against recombinant fibrillin-1 protein in Caucasian systemic sclerosis patients. Because the correct three-dimensional folding of the recombinant proteins has been substantiated by several independent methods, we conclude that autoantibodies against correctly folded fibrillin are not a primary phenomenon in the pathogenesis of systemic sclerosis.
- Published
- 2005
44. Cadherin expression pattern in melanocytic tumors more likely depends on the melanocyte environment than on tumor cell progression
- Author
-
Sven, Krengel, F, Grotelüschen, S, Bartsch, and M, Tronnier
- Subjects
Nevus, Pigmented ,Skin Neoplasms ,Ultraviolet Rays ,Disease Progression ,Humans ,Melanocytes ,Cell Count ,Cadherins ,Immunohistochemistry ,Melanoma ,Skin - Abstract
Adhesion molecules have been assigned an important role in melanocytic tumor progression. By the loss of E-cadherin, melanocytes might escape the control of neighbouring keratinocytes. Although in vitro data support this hypothesis, there are yet no conclusive immunohistochemical results on cadherin expression in melanocytic tumors.To gain detailed insight in the expression of cadherins and their cytoplasmic binding partners, the catenins, in various types of benign and malignant melanocytic neoplasms.Immunohistochemical analysis of the expression of E-, P-, and N-cadherin and alpha-, beta-, and gamma-catenin in compound and dermal nevi, Spitz nevi, blue nevi, ultraviolet B (UVB)-irradiated nevi, and malignant melanomas of various tumor thickness.In both nevi and melanomas, E-cadherin expression in melanocytic cells decreased, following a gradient from junctional to deeper dermal localization. The pattern of E-cadherin expression was more heterogeneous in melanomas than in nevi. In some melanomas, E-cadherin was only weakly positive in the epidermal tumor cells. P-cadherin expression was similar to that of E-cadherin. N-cadherin expression in melanocytic lesions was a rare finding, however, a small percentage of melanomas showed expression in some cell nests. Some Spitz nevi exhibited strong N-cadherin immunoreactivity. Most melanocytic cells were alpha- and beta-catenin-positive and gamma-catenin-negative. UVB irradiation did not influence the expression of cadherins and catenins in melanocytic nevi in vivo.It is presumed that the gradual loss of E-cadherin expression represents a reaction of melanocytic cells to altered conditions in the dermal environment, e.g. lack of contact to keratinocytes, or new contact with dermal extracellular matrix molecules, respectively. Melanoma cells apparently are less dependent on these environmental factors and, therefore, show a more heterogeneous expression pattern. This might be of importance for the adaptation of the tumor cells to local requirements. However, in view of our results, a causative role of (loss of ) E-cadherin or (gain of ) N-cadherin for melanocytic tumor progression still remains to be proven.
- Published
- 2003
45. Sepsis with bullous necrotizing skin lesions due to vibrio vulnificus acquired through recreational activities in the Baltic Sea
- Author
-
A. Heer-Sonderhoff, Werner Solbach, S. Fetscher, Sven Krengel, and K. Kuhnt-Lenz
- Subjects
Microbiology (medical) ,Cefotaxime ,Bacteremia ,Vibrio vulnificus ,Biology ,Opportunistic Infections ,Severity of Illness Index ,Microbiology ,Sepsis ,Vibrionaceae ,Vibrio Infections ,medicine ,Humans ,Blood culture ,Swimming ,integumentary system ,medicine.diagnostic_test ,Skin Diseases, Vesiculobullous ,Lymphoma, Non-Hodgkin ,fungi ,General Medicine ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Anti-Bacterial Agents ,Infectious Diseases ,Treatment Outcome ,Immunology ,bacteria ,Gentamicin ,Drug Therapy, Combination ,Female ,medicine.drug ,Follow-Up Studies - Abstract
This report describes the case of a 59-year-old woman with a history of non-Hodgkin's lymphoma who developed bacteremia with Vibrio vulnificus. The patient had been swimming in the unusually warm Baltic Sea in the summer of 2002. She presented with symptoms of septicemia and severe bullous necrotizing skin lesions of the extremities. Blood culture revealed Vibrio vulnificus as the pathogenic organism. Under treatment with cefotaxime and gentamicin, she recovered slowly without further complications. Vibrio vulnificus is a marine bacterium that is present in aquatic ecosystems worldwide, especially when water temperatures exceed 20 degrees C. Infections with Vibrio vulnificus are uncommon in Europe, and most cases are reported from subtropical or tropical regions.
- Published
- 2003
46. Familiäre Krebssyndrome
- Author
-
Helmut H. Wolff and Sven Krengel
- Published
- 2003
47. MMP-2, TIMP-2 and MT1-MMP are differentially expressed in lesional skin of melanocytic nevi and their expression is modulated by UVB-light
- Author
-
Sven, Krengel, M, Alexander, J, Brinckmann, and M, Tronnier
- Subjects
Adult ,Keratinocytes ,Nevus, Pigmented ,Tissue Inhibitor of Metalloproteinase-2 ,Skin Neoplasms ,Matrix Metalloproteinases, Membrane-Associated ,Ultraviolet Rays ,Down-Regulation ,Metalloendopeptidases ,Middle Aged ,Immunoenzyme Techniques ,Humans ,Matrix Metalloproteinase 2 ,Aged ,Skin - Abstract
In malignant melanoma, recent studies have demonstrated an important role of matrix-metalloproteinase 2 (MMP-2), its co-activating enzyme membrane-type matrix-metalloproteinase 1 (MT1-MMP), and the endogenous inhibitor of MMP-2, tissue-inhibitor of matrix metalloproteinase 2 (TIMP-2). Melanocytic nevi are benign neoplasms of the melanocytic lineage, but may exhibit dysplastic features that can be difficult to distinguish from early stage melanoma. As shown in earlier studies, nevi show important morphological and phenotypical changes in response to ultraviolet light (UVB) irradiation.To clarify the role of MMP-2, TIMP-2 and MT1-MMP in UVB-irradiated vs. non-irradiated melanocytic nevi.Immunohistochemical comparison of the MMP-2, TIMP-2 and MT1-MMP expression pattern.MMP-2 is expressed by lesional keratinocytes and its expression is up-regulated by UVB-irradiation. MMP-2 expression was not observed in melanocytic cells. TIMP-2, by contrast, is predominantly expressed by melanocytic nevus cells, and its expression is in part down-regulated by UVB-irradiation. MT1-MMP is expressed by basal keratinocytes and to a weaker extent by melanocytic nevus cells.MMP-2 expression by keratinocytes in nevi probably represents the result of activation of keratinocyte turnover in lesional epidermis. MMP-2 could play a role in the downward movement of junctional nevus cells into the dermis. The reduction of TIMP-2 expression in melanocytic cells by UV-light together with the enhanced expression of MMP-2 in the adjacent epidermis may promote basement membrane degradation. The expression pattern of MT1-MMP in close proximity to epithelial-mesenchymal interfaces underlines the synergistic role of MT1-MMP in this process.
- Published
- 2002
48. Wearing sunglasses--a risk factor for the development of cutaneous malignant melanoma?
- Author
-
Sven Krengel
- Subjects
medicine.medical_specialty ,Skin Neoplasms ,business.industry ,Melanoma ,Health Behavior ,Dermatology ,medicine.disease ,Eye Protective Devices ,Risk Factors ,medicine ,Humans ,Health behavior ,Risk factor ,business - Published
- 2002
49. Allergic contact dermatitis from black tattoo
- Author
-
Sven Krengel, Regina Treudler, Beate Tebbe, and Constantin E. Orfanos
- Subjects
Adult ,Male ,medicine.medical_specialty ,Allergy ,Tattooing ,business.industry ,Dermatology ,Pigments, Biological ,medicine.disease ,Immunopathology ,Dermatitis, Allergic Contact ,Inorganic pigments ,medicine ,Immunology and Allergy ,Humans ,business ,Allergic contact dermatitis ,Contact dermatitis ,Skin - Published
- 1998
50. Rheumatoid Nodules Developing under Methotrexate Treatment for Rheumatoid Arthritis
- Author
-
Christos C. Zouboulis, Ulrike Blume-Peytavi, C. E. Orfanos, T. N. Y. Duong, and Sven Krengel
- Subjects
Methotrexate treatment ,medicine.medical_specialty ,business.industry ,Rheumatoid arthritis ,Medicine ,Rheumatoid nodule ,Dermatology ,General Medicine ,medicine.symptom ,business ,medicine.disease - Published
- 1999
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