14 results on '"Suzi P. de Carvalho"'
Search Results
2. Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
- Author
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Suzi P. de Carvalho, Jéssica B. de Almeida, Yasmin M.F.S. Andrade, Lucas S.C. da Silva, Arianne C. de Oliveira, Flávia S. Nascimento, Guilherme B. Campos, Márcio V. Oliveira, Jorge Timenetsky, and Lucas M. Marques
- Subjects
Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three clonal complexes (CC): 5, 45, and 398. Four spa types were found circulating in this setting. Resistance of S. aureus isolates to ampicillin, erythromycin, ciprofloxacin, clindamycin, and tetracycline was 80%, 32.8%, 7.1%, 7.1% and 4.3%, respectively. One isolate presented intermediate resistance to vancomycin detected by Etest methodology. All strains were biofilm producers. The virulence genes seb, sec, spa, and pvl were detected in some isolates. This study revealed a high rate of children carrying MRSA among healthy attendees in daycare centers in Vitória da Conquista, Brazil. Keywords: CA-MRSA, Antibiotic resistance, SCCmec, MLST
- Published
- 2017
- Full Text
- View/download PDF
3. Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
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Regiane Yatsuda, Camila Dutra Barbosa, Thamara Louisy Santos Brito, Ana Paula Trovatti Uetanabaro, Jéssica Bomfim de Almeida, Hellen Braga Martins Oliveira, Lucas Miranda Marques, Caline Novais Teixeira Oliveira, Palloma Porto Almeida, Guilherme Barreto Campos, Jully Chayra Santos Viana, Mariana Morais Teixeira, Suzi P. de Carvalho, Jorge Timenetsky, Yasmin Monara Ferreira de Sousa Andrade, Hanna Izadora Laís Novaes Coelho, Manoel Neres Santos Junior, Nathan das Neves Selis, Letícia de Oliveira da Silva, Rafaela de Souza Bittencourt, Beatriz Almeida Sampaio, and Icaro Bonyek Santos da Silva
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Methicillin-Resistant Staphylococcus aureus ,medicine.drug_class ,Virulence Factors ,Science ,Acyclic Monoterpenes ,Antibiotics ,Virulence ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Citral ,Microbiology ,Article ,Minimum inhibitory concentration ,chemistry.chemical_compound ,medicine ,Multidisciplinary ,Minimum bactericidal concentration ,Microscopy, Confocal ,biochemical phenomena, metabolism, and nutrition ,Antimicrobial ,Methicillin-resistant Staphylococcus aureus ,Anti-Bacterial Agents ,chemistry ,Staphylococcus aureus ,Biofilms ,Medicine ,Pathogens ,REAÇÃO EM CADEIA POR POLIMERASE - Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
- Published
- 2020
4. Prevalence of methicillin-resistant Staphylococcus aureus colonization among healthcare workers at a tertiary care hospital in northeastern Brazil
- Author
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Kátia Regina Netto dos Santos, Yasmin M.F.S. Andrade, Guilherme Barreto Campos, Suzelle S. Mafra, Lucas Miranda Marques, Flávia S. Nascimento, Arianne C. de Oliveira, Manoel Neres Santos Junior, Suzi P. de Carvalho, Tamara S. Cunha, Raiane Cardoso Chamon, Beatriz C. Cunha, Jéssica Bomfim de Almeida, Lucas S.C. da Silva, and Eliana G. Oliveira
- Subjects
Molecular epidemiology ,SCCmec ,lcsh:Public aspects of medicine ,Resistance ,Virulence ,lcsh:RA1-1270 ,MRSA ,Biology ,S. aureus ,medicine.disease_cause ,Methicillin-resistant Staphylococcus aureus ,Microbiology ,lcsh:Infectious and parasitic diseases ,Antibiotic resistance ,Staphylococcus aureus ,Pulsed-field gel electrophoresis ,medicine ,Colonization ,lcsh:RC109-216 ,Original Research Article ,Gene expression ,Genes of virulence - Abstract
Summary Background Staphylococcus aureus is a human pathogen of clinical importance related to a variety of infections. Aim The objective of this study was to analyze the molecular and epidemiological characteristics of S. aureus obtained from healthcare professionals (HCP) of a hospital in southwestern Bahia, Brazil. Methods Samples were collected from hands, nasal cavity, and laboratory coats of 80 HCP. The bacterial isolates recovered from 240 samples were identified as S. aureus, and then analyzed for their antimicrobial resistance profile, genotypic characterization, and pathogenicity. Findings 178 isolates were identified as S. aureus, being mostly isolated from the nasal cavity. Thirty isolates (16.8%) were characterized as MRSA. The virulence gene frequency varied according to isolate source. All virulence genes were identified in at least one hand isolate. Isolates from laboratory coats did not show seb and pvl. Isolates from the nasal cavity did not exhibit pvl. The SCCmec type I was identified in 56.7% of MRSA isolates. Among MRSA isolates, 14 PFGE pulsotypes were characterized, with profile A being predominant (nine isolates). Clonal complexes CC5, CC45, and CC398 were found. MRSA isolates induced cytokine gene expression in macrophages, with IL-10 and IL-17 being expressed more often. Conclusion We found a high colonization rate for S. aureus among HCP. Moreover, we observed that MRSA strains presented different virulence factors and could induce cytokine gene expression, indicating an urgent need to control colonization rates of HCP by MRSA isolates in order to protect hospital patients and the general public.
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- 2020
5. Molecular characterization of methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates from human milk samples in Brazil
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Lucas S.C. da Silva, Yasmin M.F.S. Andrade, Raiane Cardoso Chamon, Kátia Regina Netto dos Santos, Suzi P. de Carvalho, Jéssica Bomfim de Almeida, and Lucas Miranda Marques
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Methicillin-Resistant Staphylococcus aureus ,medicine.medical_specialty ,Staphylococcus aureus ,Genotype ,Virulence ,Biology ,Breast milk ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Medical microbiology ,Bacterial Proteins ,Media Technology ,medicine ,Food microbiology ,Humans ,Pathogen ,030304 developmental biology ,0303 health sciences ,Milk, Human ,030306 microbiology ,SCCmec ,food and beverages ,Genetic Variation ,Staphylococcal Infections ,Trans-Activators ,Methicillin Resistance ,Clinical Microbiology - Short Communication ,Brazil - Abstract
Human milk is the best nutrient for infants. The donor human milk is stored in a milk bank before pasteurization. However, the human milk is not sterile and could be colonized with different types of bacteria. Many studies have shown S. aureus to be the most prevalent potential pathogen detected in human milk. This study characterized 22 methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates from raw human milk for the presence of virulence genes and agr type. Moreover, the genotypic as identified characterization was realized. The presence of virulence genes sei, seg, sec, seh, and etb was identified in resistant and sensitive strains. We observed the predominance of agr type II. The presence of SCCmec IV (67%, 4/6) and V (33%, 2/6) characterized resistant strains as CA-MRSA. Endemic lineages detected (ST1635/CC5-t002, ST5/CC5-t002, ST72/CC5-t126, ST1/CC1-t127, ST45/CC45-t065, and ST398/t1451) could be related to epidemic clones, such as USA800/ST5, USA700/ST72, USA400/ST1, USA600/ST45, and ST398. This study made it possible to understand the characteristics of virulence and clonality of some strains that circulate in breast milk in our region. The discovery of human milk colonization by MSSA and MRSA strains with molecular characteristics similar to infectious clones spread globally demonstrates the importance of monitoring strains that can spread and cause serious infections.
- Published
- 2020
6. Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil
- Author
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Lucas Miranda Marques, Lucas S.C. da Silva, Jéssica Bomfim de Almeida, Yasmin M.F.S. Andrade, Kátia Regina Netto dos Santos, Raiane Cardoso Chamon, and Suzi P. de Carvalho
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Microbiology (medical) ,HA-MRSA ,Genotype ,Virulence Factors ,CA-MRSA ,Group ii ,lcsh:QR1-502 ,Biology ,Molecular typing ,medicine.disease_cause ,lcsh:Microbiology ,lcsh:Infectious and parasitic diseases ,Microbiology ,law.invention ,03 medical and health sciences ,Similarity (network science) ,law ,medicine ,Humans ,lcsh:RC109-216 ,Gene ,Equipment and Supplies, Hospital ,Cross Infection ,0303 health sciences ,030306 microbiology ,SCCmec ,Staphylococcal Infections ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Methicillin-resistant Staphylococcus aureus ,Intensive care unit ,Community-Acquired Infections ,Intensive Care Units ,Infectious Diseases ,Community setting ,sense organs ,Brazil - Abstract
This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region. Keywords: Methicillin-resistant Staphylococcus aureus, CA-MRSA, HA-MRSA, Molecular typing
- Published
- 2019
7. Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
- Author
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Flávia S. Nascimento, Jéssica Bomfim de Almeida, Suzi P. de Carvalho, Guilherme Barreto Campos, Yasmin M.F.S. Andrade, Márcio Vasconcelos Oliveira, Lucas Miranda Marques, Lucas S.C. da Silva, Arianne C. de Oliveira, and Jorge Timenetsky
- Subjects
0301 basic medicine ,Microbiology (medical) ,Male ,Methicillin-Resistant Staphylococcus aureus ,Genotype ,Virulence Factors ,Antibiotic resistance ,CA-MRSA ,030106 microbiology ,lcsh:QR1-502 ,SCCmec ,Biology ,Nose ,Staphylococcal infections ,medicine.disease_cause ,lcsh:Microbiology ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Disk Diffusion Antimicrobial Tests ,medicine ,Humans ,lcsh:RC109-216 ,Child ,Etest ,STAPHYLOCOCCUS ,Infant ,Child Day Care Centers ,Staphylococcal Infections ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,bacterial infections and mycoses ,Methicillin-resistant Staphylococcus aureus ,Virology ,Anti-Bacterial Agents ,Community-Acquired Infections ,Infectious Diseases ,Staphylococcus aureus ,Biofilms ,Child, Preschool ,Vancomycin ,Multilocus sequence typing ,Female ,medicine.drug ,MLST - Abstract
Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three clonal complexes (CC): 5, 45, and 398. Four spa types were found circulating in this setting. Resistance of S. aureus isolates to ampicillin, erythromycin, ciprofloxacin, clindamycin, and tetracycline was 80%, 32.8%, 7.1%, 7.1% and 4.3%, respectively. One isolate presented intermediate resistance to vancomycin detected by Etest methodology. All strains were biofilm producers. The virulence genes seb, sec, spa, and pvl were detected in some isolates. This study revealed a high rate of children carrying MRSA among healthy attendees in daycare centers in Vitória da Conquista, Brazil. Keywords: CA-MRSA, Antibiotic resistance, SCCmec, MLST
- Published
- 2017
8. Draft Genome Sequence of Methicillin-Resistant
- Author
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Jéssica B, de Almeida, Suzi P, de Carvalho, Leandro M, de Freitas, Ana Marcia S, Guimarães, Naíla C, do Nascimento, Andrea P, Dos Santos, Joanne B, Messick, Jorge, Timenetsky, and Lucas M, Marques
- Subjects
Prokaryotes - Abstract
Here, we report the draft genome sequence of Staphylococcus aureus strain LC33, isolated from human breast milk in Brazil. This microorganism has been typed as ST1/t127/sccmecV. To our knowledge, this is the first draft genome sequence of a methicillin-resistant S. aureus strain isolated from human breast milk.
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- 2017
9. Draft Genome Sequences of Two Clinical Methicillin-Resistant
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Suzi P, de Carvalho, Jéssica B, de Almeida, Leandro M, de Freitas, Ana Marcia S, Guimaraes, Naíla C, do Nascimento, Andrea P, Dos Santos, Joanne B, Messick, Jorge, Timenetsky, and Lucas M, Marques
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Prokaryotes ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses - Abstract
We report here the draft genome sequences of two community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, C18 and C80, isolated from healthy children from day care centers. To our knowledge, these are the first draft genome sequences of CA-MRSA ST398/CC398/SccmecV and CA-MRSA ST5/CC5/SccmecIVa isolated from healthy children in Brazil.
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- 2017
10. Draft genome sequences of two clinical methicillin-resistant Staphylococcus aureus strains isolated from healthy children in Brazil
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Lucas Miranda Marques, Naíla C. do Nascimento, Andrea Pires dos Santos, Jorge Timenetsky, Suzi P. de Carvalho, Jéssica Bomfim de Almeida, Joanne B. Messick, Ana Marcia Sá Guimarães, and Leandro Martins de Freitas
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0301 basic medicine ,030106 microbiology ,biochemical phenomena, metabolism, and nutrition ,Biology ,medicine.disease_cause ,ANTIBIÓTICOS ,Genome ,Methicillin-resistant Staphylococcus aureus ,Microbiology ,03 medical and health sciences ,Staphylococcus aureus ,Genetics ,medicine ,Molecular Biology - Abstract
We report here the draft genome sequences of two community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, C18 and C80, isolated from healthy children from day care centers. To our knowledge, these are the first draft genome sequences of CA-MRSA ST398/CC398/Scc mec V and CA-MRSA ST5/CC5/Scc mec IVa isolated from healthy children in Brazil.
- Published
- 2017
11. Draft genome sequence of methicillin-resistant Staphylococcus aureus strain LC33 isolated from human breast milk
- Author
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Andrea Pires dos Santos, Jéssica Bomfim de Almeida, Naíla C. do Nascimento, Joanne B. Messick, Jorge Timenetsky, Lucas Miranda Marques, Ana Marcia Sá Guimarães, Suzi P. de Carvalho, and Leandro Martins de Freitas
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0301 basic medicine ,Whole genome sequencing ,Strain (biology) ,030106 microbiology ,Biology ,medicine.disease_cause ,Methicillin-resistant Staphylococcus aureus ,RESISTÊNCIA MICROBIANA ÀS DROGAS ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Staphylococcus aureus ,Genetics ,medicine ,Molecular Biology ,Human breast milk - Abstract
Here, we report the draft genome sequence of Staphylococcus aureus strain LC33, isolated from human breast milk in Brazil. This microorganism has been typed as ST1/t127/sccmecV. To our knowledge, this is the first draft genome sequence of a methicillin-resistant S. aureus strain isolated from human breast milk.
- Published
- 2017
12. Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection with Staphylococcus aureus in a Model Air Pouch
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Suzi P. de Carvalho, Hellen Braga Martins, Lucas Miranda Marques, Regiane Yatsuda, Lorena D'Oliveira Gusmão, Ana Paula Trovatti Uetanabaro, Anne Karoline Pereira Brito, Flávia S. Nascimento, Samira Itana de Souza, Jannine Dos Santos Nascimento, Nathan das Neves Selis, Clarissa Leal Silva e Souza, Márcio Vasconcelos Oliveira, and Jorge Timenetsky
- Subjects
0301 basic medicine ,Article Subject ,medicine.drug_class ,Antibiotics ,Biology ,medicine.disease_cause ,Citral ,Anti-inflammatory ,Microbiology ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,law ,Gene expression ,medicine ,Essential oil ,EXPRESSÃO GÊNICA ,lcsh:Other systems of medicine ,Antimicrobial ,lcsh:RZ201-999 ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Staphylococcus aureus ,Tumor necrosis factor alpha ,Research Article - Abstract
This study proposes to implement an alternative and effective strategy for local treatment of disease provoked byS. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal’s blood, and cytokines IL-1β, IL-6, and TNF-αwere titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, andS. aureuswas quantified using qPCR. Our results,p<0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-αlevels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, andS. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.
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- 2017
- Full Text
- View/download PDF
13. Detection, antibiotic resistance, and pathogenicity of staphylococci in samples from a Brazilian human milk bank
- Author
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Guilherme Barreto Campos, Jéssica Bomfim de Almeida, Jorge Timenetsky, Márcio Vasconcelos Oliveira, Lucas Miranda Marques, André Luís Vivas de Almeida, and Suzi P. de Carvalho
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Male ,Staphylococcus ,Human milk bank ,Breastfeeding ,Pasteurization ,Guidelines as Topic ,Microbial Sensitivity Tests ,Staphylococcal infections ,medicine.disease_cause ,Pediatrics ,Microbiology ,law.invention ,Antibiotic resistance ,law ,Pregnancy ,Maternity and Midwifery ,medicine ,Prevalence ,Humans ,Milk Banks ,Milk, Human ,business.industry ,Health Policy ,Infant, Newborn ,Obstetrics and Gynecology ,MICROBIOLOGIA ,Drug Resistance, Microbial ,Staphylococcal Infections ,medicine.disease ,Female ,business ,Brazil - Published
- 2014
14. Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
- Author
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Hellen Braga Martins Oliveira, Nathan das Neves Selis, Beatriz Almeida Sampaio, Manoel Neres Santos Júnior, Suzi Pacheco de Carvalho, Jéssica Bomfim de Almeida, Palloma Porto Almeida, Icaro Bonyek Santos da Silva, Caline Novais Teixeira Oliveira, Thamara Louisy Santos Brito, Letícia de Oliveira da Silva, Mariana Morais Teixeira, Hanna Izadora Laís Novaes Coelho, Camila Dutra Barbosa, Yasmin Monara Ferreira de Sousa Andrade, Rafaela de Souza Bittencourt, Jully Chayra Santos Viana, Guilherme Barreto Campos, Jorge Timenetsky, Ana Paula T. Uetanabaro, Regiane Yatsuda, and Lucas Miranda Marques
- Subjects
Medicine ,Science - Abstract
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
- Published
- 2021
- Full Text
- View/download PDF
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