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Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021), Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2020
-
Abstract
- Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
medicine.drug_class
Virulence Factors
Science
Acyclic Monoterpenes
Antibiotics
Virulence
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Citral
Microbiology
Article
Minimum inhibitory concentration
chemistry.chemical_compound
medicine
Multidisciplinary
Minimum bactericidal concentration
Microscopy, Confocal
biochemical phenomena, metabolism, and nutrition
Antimicrobial
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
chemistry
Staphylococcus aureus
Biofilms
Medicine
Pathogens
REAÇÃO EM CADEIA POR POLIMERASE
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....445b7059704c308f57b75a801dd285b0