1. Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus.
- Author
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Lin H, Ma Z, Hou X, Chen L, and Fan H
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Neutralizing blood, Antibodies, Viral blood, Body Temperature, Epitopes chemistry, Epitopes immunology, Interferon-gamma blood, Interleukin-4 blood, Lung pathology, Peptide Library, Peptides chemistry, Peptides immunology, Porcine Reproductive and Respiratory Syndrome metabolism, Porcine Reproductive and Respiratory Syndrome prevention & control, Porcine respiratory and reproductive syndrome virus genetics, Porcine respiratory and reproductive syndrome virus immunology, RNA, Viral blood, Suipoxvirus genetics, Suipoxvirus metabolism, Swine, Viral Vaccines genetics, Viral Vaccines immunology, Viral Vaccines metabolism, Epitopes genetics, Peptides genetics, Porcine respiratory and reproductive syndrome virus metabolism, Suipoxvirus immunology
- Abstract
To characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7. Of these, A2 and A7 recognize the mimotope, P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. The results suggest that the recombinant rSPV-mp2 provided pigs with significant protection against PRRSV infection.
- Published
- 2017
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