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A novel vaccine against Streptococcus equi ssp. zooepidemicus infections: the recombinant swinepox virus expressing M-like protein.
- Source :
-
Vaccine [Vaccine] 2011 Sep 16; Vol. 29 (40), pp. 7027-34. Date of Electronic Publication: 2011 Jul 30. - Publication Year :
- 2011
-
Abstract
- To develop a safer, more immunogenic and efficacious vaccine against Streptococcus equi ssp. zooepidemicus (SEZ) infections, the gene of M-like protein (SzP) was placed under the strong vaccinia virus promoter P28 and then inserted into swinepox virus (SPV) genome. The recombinant swinepox virus (rSPV-szp) was isolated in a non-selective medium by the co-expression of Escherichia coli LacZ gene and verified by PCR, western blotting and immunofluorescence assays. To evaluate the immunogenicity of this rSPV-szp, ICR mice were immunized with the rSPV-szp, inactivated SEZ vaccine (positive control), wild type SPV (negative control), or PBS (challenge control). All mice were intraperitoneally challenged with 5 LD(50) of homogenous ATCC 35246 strain 14 days post-vaccination. The results showed that at least 70% mice in rSPV-szp-vaccinated group were protected against homogenous ATCC 35246 challenge, the survival rate was significantly higher compared with mice in the negative control group and the challenge control group (P<0.001). The antibody titers of the rSPV-szp-vaccinated group were significantly higher (P<0.05) than the other three groups. Passive immune protection assays showed that the hyperimmune sera against M-like protein could provide mice with complete protection against challenge of ATCC 35246. Semi-quantitative RT-PCR analysis showed a marked increased in levels of IL-4 and IFN-γ mRNA in immunized mice. The results suggested that the recombinant rSPV-szp provided mice with significant protection from the SEZ infections. It is a promising candidate for the vaccine development against SEZ infections.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Bacterial immunology
Cells, Cultured
Escherichia coli genetics
Female
Immunity, Cellular immunology
Immunization, Passive methods
Interferon-gamma genetics
Interferon-gamma immunology
Interleukin-4 genetics
Interleukin-4 immunology
Mice
Mice, Inbred ICR
Plasmids genetics
Promoter Regions, Genetic
Staphylococcal Vaccines pharmacology
Suipoxvirus genetics
Swine
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Vaccines, Synthetic pharmacology
Vaccinia virus genetics
Vaccinia virus immunology
Viral Proteins genetics
Viral Proteins immunology
Antigens, Bacterial immunology
Bacterial Outer Membrane Proteins immunology
Carrier Proteins immunology
Staphylococcal Vaccines genetics
Staphylococcal Vaccines immunology
Streptococcal Infections immunology
Streptococcal Infections prevention & control
Streptococcus equi immunology
Suipoxvirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 29
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 21807055
- Full Text :
- https://doi.org/10.1016/j.vaccine.2011.07.074