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5 results on '"Straticell, Science Park Crealys"'

1. Deletion of TNFAIP6 Gene in Human Keratinocytes Demonstrates a Role for TSG-6 to Retain Hyaluronan Inside Epidermis

Author

David Bergerat, E. Faway, Olivier Svensek, Bruno Flamion, Catherine Lambert de Rouvroit, Michel Salmon, Olivier De Backer, Hélène Le-Buanec, Valérie De Glas, Yves Poumay, C. Evrard, Evelyne De Vuyst, Université de Namur [Namur] (UNamur), Inovarion, Straticell, Science Park Crealys, Immunologie humaine, physiopathologie & immunothérapie (HIPI (UMR_S_976 / U976)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Le Buanec, Hélène, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)

Subjects
Cell type, HA, hyaluronan, Th, T helper type, Inflammation, Dermatology, Extracellular matrix, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, HAS, hyaluronan synthase, Secretion, GEO, Gene Expression Omnibus, RHE, reconstructed human epidermis, 030304 developmental biology, TSG-6, 0303 health sciences, Epidermis (botany), biology, Chemistry, RNA-seq, RNA sequencing, KC, keratinocyte, AD, atopic dermatitis, crRNA, CRISPR RNA, [SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology, ECM, extracellular matrix, Cell biology, Hyaluronan synthase, medicine.anatomical_structure, RL1-803, biology.protein, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Original Article, medicine.symptom, Keratinocyte, KLK, kallikrein, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Abstract

International audience; TSG-6 is a soluble protein secreted in the extracellular matrix by various cell types in response to inflammatory stimuli. TSG-6 interacts with extracellular matrix molecules, particularly hyaluronan (HA), and promotes cutaneous wound closure in mice. Between epidermal cells, the discrete extracellular matrix contains HA and a tiny amount of TSG-6. However, challenges imposed to keratinocytes in reconstructed human epidermis revealed strong induction of TSG-6 expression, after exposure to T helper type 2 cytokines to recapitulate the atopic dermatitis phenotype or after fungal infection that causes secretion of cytokines and antimicrobial peptides. After both types of challenge, enhanced release of TSG-6 happens simultaneously with increased HA production. TSG-6 deficiency in N/TERT keratinocytes was created by inactivating TNFAIP6 using CRISPR/Cas9. Some TSG-6–/– keratinocytes analyzed through scratch assays tend to migrate more slowly but produce reconstructed human epidermis that exhibits normal morphology and differentiation. Few significant alterations were noticed by transcriptomic analysis. Nevertheless, reduced HA content in TSG-6–/– reconstructed human epidermis was observed, along with enhanced HA release into the culture medium, and this phenotype was even more pronounced after the challenging conditions. Reintroduction of cells producing TSG-6 in reconstructed human epidermis reduced HA leakage. Our results show a role for TSG-6 in sequestering HA between epidermal cells in response to inflammation.

Published
2021
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2. MARK-AGE biomarkers of ageing

Author

Jürgen Bernhard, Mikko Hurme, P. Eline Slagboom, Paola Caiafa, Tilman Grune, Alexander Bürkle, Olivier Toussaint, Michel Salmon, Daniela Gradinaru, Eugenio Mocchegiani, Efstathios S. Gonos, Ewa Sikora, Antti Hervonen, Claudio Franceschi, Peter Kristensen, Martijn E.T. Dollé, Sebastiano Collino, Beatrix Grubeck-Loebenstein, Maria A. Blasco, Duncan Talbot, Andreas Simm, Richard Aspinall, Claude Libert, Helen R. Griffiths, Jan H.J. Hoeijmakers, Gerben Zondag, Bertrand Friguet, Miriam Capri, Nicolle Breusing, Maria Moreno-Villanueva, University of Konstanz, BioTeSys GmbH, Esslingen, Spanish National Cancer Research Center (CNIO), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Université de Namur [Namur] (UNamur), Institute for Biomedical Aging Research, University of Innsbruck, Translational Research Center of Nutrition and Ageing (IRCCS-INRCA), Nestlé Institute of Health Sciences SA [Lausanne, Switzerland], National Hellenic Research Foundation, Nencki Institute of Experimental Biology, National Institute of Geriatrics and Gerontology 'Ana Aslan', National Institute for Public Health and the Environment [Bilthoven] (RIVM), Straticell, Science Park Crealys, Department of Engineering – BCE Protein Engineering, School of Life and Health Sciences, Aston University [Birmingham], Department for Molecular Biomedical Research (DFMBR), Universiteit Gent = Ghent University [Belgium] (UGENT)-VIB, University of Hohenheim, Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], University Hospital Halle, CIG - Interdepartmental Center 'L.Galvani', Leiden University Medical Center (LUMC), Department of Cellular Biotechnologies and Hematology, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Medical School, University of Tampere [Finland], The MARK-AGE project has received funding from the European Union’s FP7 research and innovation programme under grant agreement HEALTH-F4-2008-200880, European Project: 200880,EC:FP7:HEALTH,FP7-HEALTH-2007-A,MARK-AGE(2008), Leopold Franzens Universität Innsbruck - University of Innsbruck, Universiteit Gent = Ghent University (UGENT)-VIB, Universiteit Leiden, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bürkle, Alexander, Moreno-Villanueva, María, Bernhard, Jürgen, Blasco, María, Zondag, Gerben, Hoeijmakers, Jan H.J., Toussaint, Olivier, Grubeck-Loebenstein, Beatrix, Mocchegiani, Eugenio, Collino, Sebastiano, Gonos, Efstathios S., Sikora, Ewa, Gradinaru, Daniela, Dollé, Martijn, Salmon, Michel, Kristensen, Peter, Griffiths, Helen R., Libert, Claude, Grune, Tilman, Breusing, Nicolle, Simm, Andrea, Franceschi, Claudio, Capri, Miriam, Talbot, Duncan, Caiafa, Paola, Friguet, Bertrand, Slagboom, P. Eline, Hervonen, Antti, Hurme, Mikko, Aspinall, Richard, Molecular Genetics, Spanish National Cancer Research Centre (CNIO), Université de Namur [Namur], Ghent University [Belgium] (UGENT)-VIB, Friedrich-Schiller-Universität Jena, Università degli Studi di Roma 'La Sapienza' [Rome], and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Gerontology, Aging, MITOCHONDRIAL-DNA, Biological age, Single measurement, Scientific literature, Biology, 03 medical and health sciences, OLD-AGE, 0302 clinical medicine, Ageing biomarker, T-CELL SUBSETS, ddc:570, Humans, Ageing biomarkers, European commission, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, European Union, OXIDATIVE STRESS, 030304 developmental biology, Genetics, 0303 health sciences, INDUCED PREMATURE SENESCENCE, Human studies, Biology and Life Sciences, Human studie, APOLIPOPROTEIN-E GENOTYPE, REPLICATIVE SENESCENCE, MARK-AGE, Ageing, C-REACTIVE-PROTEIN, Human longevity, TELOMERE LENGTH, Female, Biomarkers, HUMAN LONGEVITY, 030217 neurology & neurosurgery, Developmental Biology
Abstract

Many candidate biomarkers of human ageing have been proposed in the scientific literature but in all cases their variability in cross-sectional studies is considerable, and therefore no single measurement has proven to serve a useful marker to determine, on its own, biological age. A plausible reason for this is the intrinsic multi-causal and multi-system nature of the ageing process. The recently completed MARK-AGE study was a large-scale integrated project supported by the European Commission. The major aim of this project was to conduct a population study comprising about 3200 subjects in order to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any marker in isolation. (C) 2015 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Published
2015
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3. Circulating cell-free DNA in health and disease - the relationship to health behaviours, ageing phenotypes and metabolomics.

Author

Kananen L, Hurme M, Bürkle A, Moreno-Villanueva M, Bernhardt J, Debacq-Chainiaux F, Grubeck-Loebenstein B, Malavolta M, Basso A, Piacenza F, Collino S, Gonos ES, Sikora E, Gradinaru D, Jansen EHJM, Dollé MET, Salmon M, Stuetz W, Weber D, Grune T, Breusing N, Simm A, Capri M, Franceschi C, Slagboom E, Talbot D, Libert C, Raitanen J, Koskinen S, Härkänen T, Stenholm S, Ala-Korpela M, Lehtimäki T, Raitakari OT, Ukkola O, Kähönen M, Jylhä M, and Jylhävä J

Subjects
Male, Humans, Female, Middle Aged, Aged, Aging genetics, Biomarkers, Phenotype, Inflammation, Health Behavior, DNA, C-Reactive Protein, Cell-Free Nucleic Acids
Abstract

Circulating cell-free DNA (cf-DNA) has emerged as a promising biomarker of ageing, tissue damage and cellular stress. However, less is known about health behaviours, ageing phenotypes and metabolic processes that lead to elevated cf-DNA levels. We sought to analyse the relationship of circulating cf-DNA level to age, sex, smoking, physical activity, vegetable consumption, ageing phenotypes (physical functioning, the number of diseases, frailty) and an extensive panel of biomarkers including blood and urine metabolites and inflammatory markers in three human cohorts (N = 5385; 17-82 years). The relationships were assessed using correlation statistics, and linear and penalised regressions (the Lasso), also stratified by sex.cf-DNA levels were significantly higher in men than in women, and especially in middle-aged men and women who smoke, and in older more frail individuals. Correlation statistics of biomarker data showed that cf-DNA level was higher with elevated inflammation (C-reactive protein, interleukin-6), and higher levels of homocysteine, and proportion of red blood cells and lower levels of ascorbic acid. Inflammation (C-reactive protein, glycoprotein acetylation), amino acids (isoleucine, leucine, tyrosine), and ketogenesis (3-hydroxybutyrate) were included in the cf-DNA level-related biomarker profiles in at least two of the cohorts.In conclusion, circulating cf-DNA level is different by sex, and related to health behaviour, health decline and metabolic processes common in health and disease. These results can inform future studies where epidemiological and biological pathways of cf-DNA are to be analysed in details, and for studies evaluating cf-DNA as a potential clinical marker., (© 2022. The Author(s).)

Published
2023
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4. Self-rated health in individuals with and without disease is associated with multiple biomarkers representing multiple biological domains.

Author

Kananen L, Enroth L, Raitanen J, Jylhävä J, Bürkle A, Moreno-Villanueva M, Bernhardt J, Toussaint O, Grubeck-Loebenstein B, Malavolta M, Basso A, Piacenza F, Collino S, Gonos ES, Sikora E, Gradinaru D, Jansen EHJM, Dollé MET, Salmon M, Stuetz W, Weber D, Grune T, Breusing N, Simm A, Capri M, Franceschi C, Slagboom PE, Talbot DCS, Libert C, Koskinen S, Bruunsgaard H, Hansen ÅM, Lund R, Hurme M, and Jylhä M

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Young Adult, Biomarkers blood, Biomarkers urine, Diagnostic Self Evaluation, Health Status, Self Report
Abstract

Self-rated health (SRH) is one of the most frequently used indicators in health and social research. Its robust association with mortality in very different populations implies that it is a comprehensive measure of health status and may even reflect the condition of the human organism beyond clinical diagnoses. Yet the biological basis of SRH is poorly understood. We used data from three independent European population samples (N approx. 15,000) to investigate the associations of SRH with 150 biomolecules in blood or urine (biomarkers). Altogether 57 biomarkers representing different organ systems were associated with SRH. In almost half of the cases the association was independent of disease and physical functioning. Biomarkers weakened but did not remove the association between SRH and mortality. We propose three potential pathways through which biomarkers may be incorporated into an individual's subjective health assessment, including (1) their role in clinical diseases; (2) their association with health-related lifestyles; and (3) their potential to stimulate physical sensations through interoceptive mechanisms. Our findings indicate that SRH has a solid biological basis and it is a valid but non-specific indicator of the biological condition of the human organism.

Published
2021
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5. MARK-AGE biomarkers of ageing.

Author

Bürkle A, Moreno-Villanueva M, Bernhard J, Blasco M, Zondag G, Hoeijmakers JH, Toussaint O, Grubeck-Loebenstein B, Mocchegiani E, Collino S, Gonos ES, Sikora E, Gradinaru D, Dollé M, Salmon M, Kristensen P, Griffiths HR, Libert C, Grune T, Breusing N, Simm A, Franceschi C, Capri M, Talbot D, Caiafa P, Friguet B, Slagboom PE, Hervonen A, Hurme M, and Aspinall R

Subjects
European Union, Female, Humans, Male, Aging metabolism, Biomarkers metabolism
Abstract

Many candidate biomarkers of human ageing have been proposed in the scientific literature but in all cases their variability in cross-sectional studies is considerable, and therefore no single measurement has proven to serve a useful marker to determine, on its own, biological age. A plausible reason for this is the intrinsic multi-causal and multi-system nature of the ageing process. The recently completed MARK-AGE study was a large-scale integrated project supported by the European Commission. The major aim of this project was to conduct a population study comprising about 3200 subjects in order to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any marker in isolation., (Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.)

Published
2015
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