1. At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions
- Author
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Ceren Simsek, Alexander N. Lukashev, Ward Deboutte, Yaw Adu Sarkodie, Daan Jansen, Hermann Ulrich Everling, Kwe Claude Yinda, Tabea Binger, Florian Gloza-Rausch, Eric M. Leroy, Mathieu Bourgarel, Leen Beller, Victor M. Corman, Augustina Sylverken, Stoian Yordanov, Marc Van Ranst, Jan Felix Drexler, Samuel Oppong, Gael Darren Maganga, Jelle Matthijnssens, Peter Vallo, Christian Drosten, Antje Seebens-Hoyer, Andrea Rasche, Rega Institute for Medical Research [Leuven, België], Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], German Center for Infection Research, Partnersite Munich (DZIF), University of Bonn, Sechenov First Moscow State Medical University, Université des Sciences et Techniques de Masuku [Franceville, Gabon] (USTM), Centre International de Recherches Médicales de Franceville (CIRMF), Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Noctalis, Centre for Bat Protection and Information, Forestry Board Directorate of Strandja Natural Park, Strandja Natural Park, Kwame Nkrumah University of Science and Technology [GHANA] (KNUST), Institute of Vertebrate Biology of the Czech Academy of Sciences (IVB / CAS), Czech Academy of Sciences [Prague] (CAS), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie agreement no. 721367 granted to J.F.D., J.M., and M.V.R. EU Horizon 2020 projects EVAg (grant agreement number 653316) and COMPARE (agreement number 643476) granted to C.D., and the Russian Science Foundation grant 19-15-00055 to A.N.L. also provided funding to the current study. H.U.E. had a personal scholarship from the BONFOR intramural program at the University of Bonn. German Federal Ministry of Education and Research (BMBF) (project code 01KIO16D), Deutsche Forschungsgemeinschaft (DFG DR 772-3/1), Deutsche Forschungsgemeinschaft within the Africa Infectious Diseases program gave grants to C.D. and Y.A.S. (DR 772/3-1) and to S.O. (KA1241/18-1) that were also among the funding contributions. A personal scholarship granted to A.R. from the German Academic Exchange Service (DAAD) supported field work in Costa Rica. D. J. was supported by the Fonds Wetenschappelijk Onderzoek (Research foundation Flanders) (1S78019N). L.B. was supported by the Fonds Wetenschappelijk Onderzoek (1S61618N). K.C.Y. was funded by the Interfaculty Council for Development Cooperation (IRO) from the KU Leuven. The computing power in this work was provided by the VSC (Flemish Supercomputer Centre), financed by the FWO and the Flemish government, department EWI., European Project: 777657,H2020-EU.1.3.3. - Stimulating innovation by means of cross-fertilisation of knowledge,777657,MSCA-RISE(2018), European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), European Project: 643476,H2020,H2020-PHC-2014-single-stage,COMPARE(2014), Universität Bonn = University of Bonn, Université des Sciences et Techniques de Masuku (USTM), and Kwame Nkrumah University of Science and Technology (KNUST)
- Subjects
Rotavirus ,Viral metagenomics ,[SDV]Life Sciences [q-bio] ,viruses ,Reassortment ,medicine.disease_cause ,L73 - Maladies des animaux ,Genome ,Zoonoses ,Chiroptera ,Genotype ,Phylogeny ,0303 health sciences ,Phylogenetic tree ,Strain (biology) ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Zoonosis ,virus diseases ,QR1-502 ,3. Good health ,S50 - Santé humaine ,Middle East Respiratory Syndrome Coronavirus ,Research Article ,Diarrhea ,zoonose ,Middle East respiratory syndrome coronavirus ,relation homme-faune ,Genome, Viral ,Ecological and Evolutionary Science ,Biology ,Microbiology ,Rotavirus Infections ,rotavirus genetic diversity ,SA11 ,03 medical and health sciences ,Coronavirus 2 du syndrome respiratoire aigu sévère ,Virology ,medicine ,Animals ,Humans ,Horses ,Transmission des maladies ,030304 developmental biology ,Genetic diversity ,030306 microbiology ,SARS-CoV-2 ,COVID-19 ,Genetic Variation ,zoonosis ,medicine.disease ,respiratory tract diseases ,Evolutionary biology ,bat rotavirus ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Metagenomics ,Virus pathogène - Abstract
The increased research on bat coronaviruses after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) allowed the very rapid identification of SARS-CoV-2. This is an excellent example of the importance of knowing viruses harbored by wildlife in general, and bats in particular, for global preparedness against emerging viral pathogens., Bats host many viruses pathogenic to humans, and increasing evidence suggests that rotavirus A (RVA) also belongs to this list. Rotaviruses cause diarrheal disease in many mammals and birds, and their segmented genomes allow them to reassort and increase their genetic diversity. Eighteen out of 2,142 bat fecal samples (0.8%) collected from Europe, Central America, and Africa were PCR-positive for RVA, and 11 of those were fully characterized using viral metagenomics. Upon contrasting their genomes with publicly available data, at least 7 distinct bat RVA genotype constellations (GCs) were identified, which included evidence of reassortments and 6 novel genotypes. Some of these constellations are spread across the world, whereas others appear to be geographically restricted. Our analyses also suggest that several unusual human and equine RVA strains might be of bat RVA origin, based on their phylogenetic clustering, despite various levels of nucleotide sequence identities between them. Although SA11 is one of the most widely used reference strains for RVA research and forms the backbone of a reverse genetics system, its origin remained enigmatic. Remarkably, the majority of the genotypes of SA11-like strains were shared with Gabonese bat RVAs, suggesting a potential common origin. Overall, our findings suggest an underexplored genetic diversity of RVAs in bats, which is likely only the tip of the iceberg. Increasing contact between humans and bat wildlife will further increase the zoonosis risk, which warrants closer attention to these viruses.
- Published
- 2021