1. Apple polyphenols extract (APE) improves colon damage in a rat model of colitis
- Author
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Giuseppe D'Argenio a, b, Giovanna Mazzone a, Concetta Tuccillo c, Maria T. Ribecco a, Giulia Graziani d, Antonietta G. Gravina c, Sergio Caserta e, f, Stefano Guido e, Vincenzo Fogliano d, Nicola Caporaso a, Marco Romano c, D’Argenio, G., Mazzone, G., Tuccillo, C., Ribecco, Mt, Graziani, G., Gravina, Ag, Caserta, S., Guido, S., Fogliano, V., Caporaso, N., Romano, Marco, D'Argenio, Giuseppe, Mazzone, Giovanna, C., Tuccillo, Ribecco, MARIA TERESA SILVIA, Graziani, Giulia, A. G., Gravina, Caserta, Sergio, Guido, Stefano, Fogliano, Vincenzo, Caporaso, Nicola, and M., Romano
- Subjects
Male ,Tissue transglutaminase ,GASTRIC-MUCOSA ,Pharmacology ,law.invention ,chemistry.chemical_compound ,Mice ,law ,Intestinal Mucosa ,medicine.diagnostic_test ,biology ,Calpain ,Gastroenterology ,INHIBITOR ,Colitis ,Ulcerative colitis ,medicine.anatomical_structure ,Biochemistry ,ULCERATIVE-COLITIS ,Malus ,Rabbits ,EXPRESSION ,Western blot ,Cystamine ,GTP-Binding Proteins ,medicine ,MANAGEMENT ,Animals ,Rat colitis ,Protein Glutamine gamma Glutamyltransferase 2 ,RNA, Messenger ,Rats, Wistar ,Fibroblast ,Transglutaminases ,Hepatology ,business.industry ,Plant Extracts ,Tumor Necrosis Factor-alpha ,Apple polyphenols ,DINITROBENZENE SULFONIC-ACID ,Polyphenols ,IN-VITRO ,medicine.disease ,chemistry ,Trinitrobenzenesulfonic Acid ,Cyclooxygenase 2 ,biology.protein ,NIH 3T3 Cells ,Phytotherapy ,business ,INFLAMMATORY-BOWEL-DISEASE - Abstract
Background and aim: Searching for alternative therapies that are effective, safe and less expensive of those currently used for ulcerative colitis, we investigated the efficacy of a polyphenol extract from apple in rat colitis. Methods: Rats with trinitrobenzensulphonic acid-induced colitis were treated daily with rectal administration of apple polyphenols 10(-4) M for 14 days. COX-2, TNF-alpha, tissue transglutaminase and calpain in colon mucosa samples were assessed by reverse transcription-polymerase chain reaction and western blot analyses. To ascertain the role of tissue transglutaminase in mucosal healing, wounded rat fibroblasts were incubated with cystamine (a tissue transglutaminase activity inhibitor). Results: Colitis was associated with increased COX-2, TNF-alpha, calpain, and tissue transglutaminase mRNA. The protein expression of COX-2, TNF-alpha and calpain was increased whilst tissue transglutaminase was decreased. Apple extract treatment reduced the severity of colitis (p < 0.05) and restored all the considered biomarkers at the baseline level. Apple polyphenols reduced the degradation of tissue transglutaminase protein occurring through calpain action. Apple polyphenols-treated wounded fibroblast recovered within 24 h showing intense immunoreactivity for tissue transglutaminase. Conclusion: The efficacy of apple extract is mediated by its effects on COX-2 and TNF-alpha. The unbalance between calpain and tissue transglutaminase may play a role in colonic damage and future therapeutic interventions in ulcerative colitis can target this mechanisms. (C) 2012 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
- Published
- 2012