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Apple polyphenols extract (APE) improves colon damage in a rat model of colitis
- Source :
- Digestive and Liver Disease 44 (2012) 7, Digestive and Liver Disease, 44(7), 555-562, Digestive and Liver Disease 44 (2012): 555–562. doi:10.1016/j.dld.2012.01.009, info:cnr-pdr/source/autori:Giuseppe D'Argenio a, b, Giovanna Mazzone a, Concetta Tuccillo c, Maria T. Ribecco a, Giulia Graziani d, Antonietta G. Gravina c, Sergio Caserta e, f, Stefano Guido e, f, Vincenzo Fogliano d, Nicola Caporaso a, Marco Romano c/titolo:Apple polyphenols extract (APE) improves colon damage in a rat model of colitis/doi:10.1016%2Fj.dld.2012.01.009/rivista:Digestive and Liver Disease/anno:2012/pagina_da:555/pagina_a:562/intervallo_pagine:555–562/volume:44
- Publication Year :
- 2012
-
Abstract
- Background and aim: Searching for alternative therapies that are effective, safe and less expensive of those currently used for ulcerative colitis, we investigated the efficacy of a polyphenol extract from apple in rat colitis. Methods: Rats with trinitrobenzensulphonic acid-induced colitis were treated daily with rectal administration of apple polyphenols 10(-4) M for 14 days. COX-2, TNF-alpha, tissue transglutaminase and calpain in colon mucosa samples were assessed by reverse transcription-polymerase chain reaction and western blot analyses. To ascertain the role of tissue transglutaminase in mucosal healing, wounded rat fibroblasts were incubated with cystamine (a tissue transglutaminase activity inhibitor). Results: Colitis was associated with increased COX-2, TNF-alpha, calpain, and tissue transglutaminase mRNA. The protein expression of COX-2, TNF-alpha and calpain was increased whilst tissue transglutaminase was decreased. Apple extract treatment reduced the severity of colitis (p < 0.05) and restored all the considered biomarkers at the baseline level. Apple polyphenols reduced the degradation of tissue transglutaminase protein occurring through calpain action. Apple polyphenols-treated wounded fibroblast recovered within 24 h showing intense immunoreactivity for tissue transglutaminase. Conclusion: The efficacy of apple extract is mediated by its effects on COX-2 and TNF-alpha. The unbalance between calpain and tissue transglutaminase may play a role in colonic damage and future therapeutic interventions in ulcerative colitis can target this mechanisms. (C) 2012 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
- Subjects :
- Male
Tissue transglutaminase
GASTRIC-MUCOSA
Pharmacology
law.invention
chemistry.chemical_compound
Mice
law
Intestinal Mucosa
medicine.diagnostic_test
biology
Calpain
Gastroenterology
INHIBITOR
Colitis
Ulcerative colitis
medicine.anatomical_structure
Biochemistry
ULCERATIVE-COLITIS
Malus
Rabbits
EXPRESSION
Western blot
Cystamine
GTP-Binding Proteins
medicine
MANAGEMENT
Animals
Rat colitis
Protein Glutamine gamma Glutamyltransferase 2
RNA, Messenger
Rats, Wistar
Fibroblast
Transglutaminases
Hepatology
business.industry
Plant Extracts
Tumor Necrosis Factor-alpha
Apple polyphenols
DINITROBENZENE SULFONIC-ACID
Polyphenols
IN-VITRO
medicine.disease
chemistry
Trinitrobenzenesulfonic Acid
Cyclooxygenase 2
biology.protein
NIH 3T3 Cells
Phytotherapy
business
INFLAMMATORY-BOWEL-DISEASE
Subjects
Details
- ISSN :
- 15908658
- Database :
- OpenAIRE
- Journal :
- Digestive and Liver Disease 44 (2012) 7, Digestive and Liver Disease, 44(7), 555-562, Digestive and Liver Disease 44 (2012): 555–562. doi:10.1016/j.dld.2012.01.009, info:cnr-pdr/source/autori:Giuseppe D'Argenio a, b, Giovanna Mazzone a, Concetta Tuccillo c, Maria T. Ribecco a, Giulia Graziani d, Antonietta G. Gravina c, Sergio Caserta e, f, Stefano Guido e, f, Vincenzo Fogliano d, Nicola Caporaso a, Marco Romano c/titolo:Apple polyphenols extract (APE) improves colon damage in a rat model of colitis/doi:10.1016%2Fj.dld.2012.01.009/rivista:Digestive and Liver Disease/anno:2012/pagina_da:555/pagina_a:562/intervallo_pagine:555–562/volume:44
- Accession number :
- edsair.doi.dedup.....be29a6bcc81a7d2c8b02de53b8951a76