1. S-nitrosoglutathione-induced toxicity in Drosophila melanogaster: Delayed pupation and induced mild oxidative/nitrosative stress in eclosed flies
- Author
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Janet M. Storey, Natalia I. Kryshchuk, Stanislava V. Skarbek, Ivan V. Maksymiv, Natalia Y. Shchypanska, Anna H. Riabkina, Oleh V. Lushchak, Oleksandr V. Lozinsky, Kenneth B. Storey, and Volodymyr I. Lushchak
- Subjects
Male ,Time Factors ,Physiology ,medicine.disease_cause ,Biochemistry ,Aconitase ,Nitric oxide ,S-Nitrosoglutathione ,Superoxide dismutase ,Andrology ,Protein Carbonylation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Drosophila Proteins ,Molecular Biology ,Nitrites ,030304 developmental biology ,Glutathione Transferase ,chemistry.chemical_classification ,Aconitate Hydratase ,0303 health sciences ,Reactive oxygen species ,biology ,fungi ,Pupa ,Feeding Behavior ,Catalase ,Isocitrate Dehydrogenase ,Culture Media ,Enzyme Activation ,Oxidative Stress ,Drosophila melanogaster ,chemistry ,Larva ,Toxicity ,biology.protein ,Female ,Reactive Oxygen Species ,030217 neurology & neurosurgery ,Oxidative stress ,Biomarkers - Abstract
The toxicity of the nitric oxide donor S-nitrosoglutathione (GSNO) was tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with GSNO at concentrations of 1.0, 1.5 or 4.0 mM. Food supplementation with GSNO caused a developmental delay in the flies. Biochemical analyses of oxidative stress markers and activities of antioxidant and associated enzymes were carried out on 2-day-old flies that emerged from control larvae and larvae fed on food supplemented with GSNO. Larval exposure to GSNO resulted in lower activities of aconitase in both sexes and also lower activities of catalase and isocitrate dehydrogenase in adult males relative to the control cohort. Larval treatment with GSNO resulted in higher carbonyl protein content and higher activities of glucose-6-phosphate dehydrogenase in males and higher activities of superoxide dismutase and glutathione-S-transferase in both sexes. Among the parameters tested, aconitase activity and developmental end points may be useful early indicators of toxicity caused by GSNO.
- Published
- 2012