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S-nitrosoglutathione-induced toxicity in Drosophila melanogaster: Delayed pupation and induced mild oxidative/nitrosative stress in eclosed flies
- Source :
- Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 164(1)
- Publication Year :
- 2012
-
Abstract
- The toxicity of the nitric oxide donor S-nitrosoglutathione (GSNO) was tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with GSNO at concentrations of 1.0, 1.5 or 4.0 mM. Food supplementation with GSNO caused a developmental delay in the flies. Biochemical analyses of oxidative stress markers and activities of antioxidant and associated enzymes were carried out on 2-day-old flies that emerged from control larvae and larvae fed on food supplemented with GSNO. Larval exposure to GSNO resulted in lower activities of aconitase in both sexes and also lower activities of catalase and isocitrate dehydrogenase in adult males relative to the control cohort. Larval treatment with GSNO resulted in higher carbonyl protein content and higher activities of glucose-6-phosphate dehydrogenase in males and higher activities of superoxide dismutase and glutathione-S-transferase in both sexes. Among the parameters tested, aconitase activity and developmental end points may be useful early indicators of toxicity caused by GSNO.
- Subjects :
- Male
Time Factors
Physiology
medicine.disease_cause
Biochemistry
Aconitase
Nitric oxide
S-Nitrosoglutathione
Superoxide dismutase
Andrology
Protein Carbonylation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Drosophila Proteins
Molecular Biology
Nitrites
030304 developmental biology
Glutathione Transferase
chemistry.chemical_classification
Aconitate Hydratase
0303 health sciences
Reactive oxygen species
biology
fungi
Pupa
Feeding Behavior
Catalase
Isocitrate Dehydrogenase
Culture Media
Enzyme Activation
Oxidative Stress
Drosophila melanogaster
chemistry
Larva
Toxicity
biology.protein
Female
Reactive Oxygen Species
030217 neurology & neurosurgery
Oxidative stress
Biomarkers
Subjects
Details
- ISSN :
- 15314332
- Volume :
- 164
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecularintegrative physiology
- Accession number :
- edsair.doi.dedup.....9e33d7f3a22cec599b76663d8675e28c