1. IgE, IgG1, and IgG4 Reactivity to Dermatophagoides pteronyssinus Glycosylated Extract in Allergic Patients.
- Author
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Resende RO, Ynoue LH, Miranda JS, de Almeida KC, Silva DAO, Sopelete MC, Alves R, Gennari-Cardoso ML, and Taketomi EA
- Subjects
- Adult, Allergens chemistry, Animals, Concanavalin A chemistry, Concanavalin A immunology, Dermatophagoides pteronyssinus chemistry, Enzyme-Linked Immunosorbent Assay, Female, Glycosylation, Healthy Volunteers, Humans, Immunoglobulin E chemistry, Immunoglobulin G chemistry, Immunoglobulin G immunology, Male, Middle Aged, Plant Extracts chemistry, Plant Extracts immunology, Pyroglyphidae chemistry, Pyroglyphidae immunology, Allergens immunology, Dermatophagoides pteronyssinus immunology, Hypersensitivity immunology, Immunoglobulin E immunology
- Abstract
Background: House dust mites are important allergen sources and some of these allergenic proteins may contain carbohydrate moieties, which are able to be isolated using lectins, as Concanavalin A (ConA). This study aimed to investigate allergenicity (IgE) and antigenicity (IgG1 and IgG4) of ConA-unbound and ConA-bound Dermatophagoides pteronyssinus (Dpt) crude extracts using sera of mite-allergic patients as well as inhibition capacity of antibody binding., Material and Methods: We obtained mannose-enriched and mannose-depleted fractions from Dpt by ConA affinity chromatography. Both ConA-bound and ConA-unbound fractions were evaluated by ELISA and Western Blotting for specific IgE, IgG1, and IgG4 reactivity with sera obtained from 95 mite-allergic patients (DP+) and 92 nonallergic (NA) subjects. Inhibition ELISA was used to assess cross-reactivity between Dpt extract and its fractions., Results: Among the DP+ patients, no difference was found between ConA-unbound and ConA-bound fractions regarding the levels of specific IgE, IgG1, and IgG4. Nonallergic subjects had the same levels of specific IgG1 to both ConA-unbound and ConA-bound fractions, although for specific IgG4, values were higher for ConA-bound. A positive correlation was found among specific IgE, IgG1, and IgG4 levels when Dpt was compared to ConA-unbound and ConA-bound fractions. Recognition of crude Dpt by IgE, IgG1, and IgG4 was highly inhibited by ConA-unbound and ConA-bound fractions. Western Blotting revealed a broad spectrum of bands ranging from 14 to 116 kDa recognized by specific IgE and IgG4. However, IgG1 reached higher frequency values on high molecular weight polypeptides., Conclusion: ConA-unbound and ConA-bound fractions derived from D. pteronyssinus crude extract revealed important components involved in the IgE recognition in allergic patients as well as IgG1 and/or IgG4 in allergic and healthy subjects., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper.
- Published
- 2019
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