Back to Search
Start Over
IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen.
- Source :
-
Parasitology research [Parasitol Res] 2007 Oct; Vol. 101 (5), pp. 1209-14. Date of Electronic Publication: 2007 Jul 04. - Publication Year :
- 2007
-
Abstract
- The aim of this study was to evaluate total IgG, IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA) using Strongyloides ratti saline extract as heterologous antigen for a possible clinical utility of the assay. A total of 40 serum samples of patients who were shedding Strongyloides stercoralis larvae in feces (group I), 30 sera from patients with other intestinal parasites (group II), and 30 sera from subjects with negative results in three parasitological assays (group III) were analyzed to detect total IgG, IgG1, IgG4, and IgE to Strongyloides spp. by ELISA and expressed in ELISA index. Levels of total IgG anti-Strongyloides spp. were significantly higher in patients of group I than in groups II (p=0.0005) and III (p<0.0001). Levels of specific IgG1, IgG4, and IgE of group I were also significantly higher than in groups II and III, respectively. There was a significant positive correlation between specific IgE and IgG4 (r=0.6524; p=0.0084) and IgG1 and IgG4 (r=0.5398; p=0.0171). It can be concluded that the detection of specific IgE, IgG1, and IgG4 subclasses rather than total IgG antibodies to Strongyloides spp. using the S. ratti antigen showed to be an additional tool for improving the serodiagnosis of human strongyloidiasis.
- Subjects :
- Animals
Antigens, Helminth isolation & purification
Feces parasitology
Humans
Larva
Strongyloides ratti chemistry
Strongyloides ratti immunology
Strongyloides stercoralis isolation & purification
Strongyloidiasis immunology
Antibodies, Helminth blood
Antigens, Heterophile isolation & purification
Enzyme-Linked Immunosorbent Assay methods
Immunoglobulin E blood
Immunoglobulin G blood
Strongyloidiasis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0932-0113
- Volume :
- 101
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 17610082
- Full Text :
- https://doi.org/10.1007/s00436-007-0602-z