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4. Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae Causing Invasive Pneumococcal Disease in Korea Between 2017 and 2019 After Introduction of the 13-Valent Pneumococcal Conjugate Vaccine

Author

Kim, Gyu Ri, primary, Kim, Eun-Young, additional, Kim, Si Hyun, additional, Lee, Hae Kyung, additional, Lee, Jaehyeon, additional, Shin, Jong Hee, additional, Kim, Young Ree, additional, Song, Sae Am, additional, Jeong, Joseph, additional, Uh, Young, additional, Kim, Yu Kyung, additional, Yong, Dongeun, additional, Kim, Hyun Soo, additional, Kim, Sunjoo, additional, Kim, Young Ah, additional, Shin, Kyeong Seob, additional, Jeong, Seok Hoon, additional, Ryoo, Namhee, additional, and Shin, Jeong Hwan, additional

Published
2022
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8. Serotype Distribution and Antimicrobial Resistance of Salmonella Isolates in Korea between 2016 and 2017

Author

Kim, Si Hyun, primary, Sung, Gyung-Hye, additional, Park, Eun Hee, additional, Hwang, In Yeong, additional, Kim, Gyu Ri, additional, Song, Sae Am, additional, Lee, Hae Kyung, additional, Uh, Young, additional, Kim, Young Ah, additional, Jeong, Seok Hoon, additional, Shin, Jong Hee, additional, Shin, Kyeong Seob, additional, Lee, Jaehyeon, additional, Jeong, Joseph, additional, Kim, Young Ree, additional, Yong, Dongeun, additional, Lee, Miae, additional, Kim, Yu Kyung, additional, Ryoo, Nam Hee, additional, Lee, Seungok, additional, Kim, Jayoung, additional, Kim, Sunjoo, additional, Kim, Hyun Soo, additional, and Shin, Jeong Hwan, additional

Published
2022
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11. Comparative Evaluation of the STANDARD F Influenza A/B FIA Test with the Sofia Influenza A+B FIA and SD BIOLINE Influenza Ag A/B/A(H1N1) tests for Influenza A Virus Detection

Author

Jeon Ga Won, Song Sae Am, Kim Si Hyun, Soohun Yoo, Jeong Hwan Shin, and Yohan Park

Subjects
business.industry, Medicine, Influenza a, fluorescence, immunoassay, General Medicine, Fluorescence immunoassay, influenza a, business, enzyme immunoassay, Microbiology, Virology, QR1-502
Abstract

배경: STANDARD F Influenza A/B FIA (SD Biosensor Inc, Korea)의 인플루엔자 A 진단능 평가를 위해 Sofia Influenza A+B FIA (Quidel Corp., USA) 와 SD BIOLINE Influenza Ag A/B/A(H1N1) (Standard Diagnostic, Inc., Korea) 와 비교하고자 하였다.방법: 호흡기 바이러스 실시간 RT-PCR이 의뢰된 중복 검체를 제외한 총 227개의 비인두 흡입물 검체를 대상으로 하였다 검사 후 남은 잔여 검체를 이용하여 제조사의 권고대로 검사를 시행하였다. 세가지 신속검사 키트의 민감도와 특이도를 포함한 진단능을 분석하였다.결과: 실시간 RT-PCR의 결과 67건(29.5%)이 인플루엔자 A 양성, 160건(70.5%)이 음성이었고 인플루엔자 B는 모두 음성이었다. 인플루엔자 A의 민감도와 특이도는 STANDARD F는 각각 50.7%와 100%, Sofia는 50.7%와 100%였으며, SD BIOLINE이 29.9%와 100%였다. STANDARD F와 SD BIOLINE은 모든 검체에서 인플루엔자 B가 음성인 반면, Sofia는 두 검체에서 위양성의 결과를 보였다.결론: STANDARD F Influenza A/B는 우수한 진단 성능을 보이며 인플루엔자 A를 신속 진단하는데 있어 유용하게 사용될 것이다.

Published
2021

17. Serotype Distribution and Antimicrobial Resistance of Invasive and Noninvasive Streptococcus pneumoniae Isolates in Korea between 2014 and 2016

Author

Park, Dong-Chul, primary, Kim, Si Hyun, additional, Yong, Dongeun, additional, Suh, In Bum, additional, Kim, Young Ree, additional, Yi, Jongyoun, additional, Song, Wonkeun, additional, Song, Sae Am, additional, Moon, Hee-Won, additional, Lee, Hae Kyung, additional, Park, Kyoung Un, additional, Kim, Sunjoo, additional, Jeong, Seok Hoon, additional, Lee, Jaehyeon, additional, Jeong, Joseph, additional, Kim, Yu Kyung, additional, Lee, Miae, additional, Cho, Jihyun, additional, Kim, Jong-Wan, additional, Shin, Kyeong Seob, additional, Hwang, Sang-Hyun, additional, Chung, Jae-Woo, additional, Woo, Hye In, additional, Lee, Chae Hoon, additional, Ryoo, Namhee, additional, Chang, Chulhun L., additional, Kim, Hyun Soo, additional, Kim, Jayoung, additional, Shin, Jong Hee, additional, Kim, Soo Hyun, additional, Lee, Mi-Kyung, additional, Lee, Seong Gyu, additional, Jang, Sook Jin, additional, Lee, Kyutaeg, additional, Suh, HunSuk, additional, Sohn, Yong-Hak, additional, Kwon, Min-Jung, additional, Lee, Hee Joo, additional, Hong, Ki Ho, additional, Woo, Kwang-Sook, additional, Park, Chul Min, additional, and Shin, Jeong Hwan, additional

Published
2019
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19. Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015

Author

Kim, Si Hyun, primary, Park, Eun Hee, additional, Hwang, In Yeong, additional, Lee, Hyukmin, additional, Song, Sae Am, additional, Lee, Miae, additional, Lee, Seungok, additional, Kim, Soo Young, additional, Kim, Jin Ju, additional, Shin, Jong Hee, additional, Hong, Seong Geun, additional, Shin, Kyeong Seob, additional, Kim, Sunjoo, additional, Ryoo, Nam Hee, additional, Lee, Woonhyoung, additional, Jang, Sook Jin, additional, and Shin, Jeong Hwan, additional

Published
2019
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22. Corrigendum to “MALDI-TOF MS is more accurate than VITEK II ANC card and API Rapid ID 32 A system for the identification of Clostridium species” [Anaerobe 40 (2016) 73–75]

Author

Kim, Young Jin, primary, Kim, Si Hyun, additional, Park, Hyun-Jung, additional, Park, Hae-Geun, additional, Park, Dongchul, additional, Song, Sae Am, additional, Lee, Hee Joo, additional, Yong, Dongeun, additional, Choi, Jun Yong, additional, Kook, Joong-Ki, additional, Kim, Hye Ran, additional, and Shin, Jeong Hwan, additional

Published
2017
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38. Postsurgical Wound Infection Caused byMycobacterium conceptionenseIdentified by Sequencing of 16S rRNA,hsp65, andrpoBGenes in an Immunocompetent Patient

Author

Lee, Ja Young, primary, Kim, Si Hyun, additional, Shin, Jeong Hwan, additional, Lee, Hyun-Kyung, additional, Lee, Young Min, additional, Song, Sae Am, additional, Bae, Il Kwon, additional, Kim, Chang-Ki, additional, Jun, Kyung Ran, additional, Kim, Hye Ran, additional, Lee, Jeong Nyeo, additional, and Chang, Chulhun L., additional

Published
2014
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40. Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae Isolates Causing Invasive and Noninvasive Pneumococcal Diseases in Korea from 2008 to 2014.

Author

Kim, Si Hyun, Bae, Il Kwon, Park, Dongchul, Lee, Kyungmin, Kim, Na Young, Song, Sae Am, Kim, Hye Ran, Jeon, Ga Won, Urm, Sang-Hwa, and Shin, Jeong Hwan

Subjects
ANTI-infective agents, STREPTOCOCCAL disease prevention, QUINOLONE antibacterial agents, CHI-squared test, DRUG resistance in microorganisms, PNEUMOCOCCAL vaccines, RESEARCH funding, STREPTOCOCCAL diseases, STREPTOCOCCUS, SEROTYPES, DATA analysis software, DESCRIPTIVE statistics, THERAPEUTICS
Abstract

Introduction. Streptococcus pneumoniae is an important pathogen with high morbidity and mortality rates. The aim of this study was to evaluate the distribution of common serotypes and antimicrobial susceptibility of S. pneumoniae in Korea. Methods. A total of 378 pneumococcal isolates were collected from 2008 through 2014. We analyzed the serotype and antimicrobial susceptibility for both invasive and noninvasive isolates. Results. Over the 7 years, 3 (13.5%), 35 (10.8%), 19A (9.0%), 19F (6.6%), 6A (6.1%), and 34 (5.6%) were common serotypes/serogroups. The vaccine coverage rates of PCV7, PCV10, PCV13, and PPSV23 were 21.4%, 23.3%, 51.9%, and 62.4% in all periods. The proportions of serotypes 19A and 19F decreased and nonvaccine serotypes increased between 2008 and 2010 and 2011 and 2014. Of 378 S. pneumoniae isolates, 131 (34.7%) were multidrug resistant (MDR) and serotypes 19A and 19F were predominant. The resistance rate to levofloxacin was significantly increased (7.2%). Conclusion. We found changes of pneumococcal serotype and antimicrobial susceptibility during the 7 years after introduction of the first pneumococcal vaccine. It is important to continuously monitor pneumococcal serotypes and their susceptibilities. [ABSTRACT FROM AUTHOR]

Published
2016
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41. Evaluation of DNA Extraction Methods and Their Clinical Application for Direct Detection of Causative Bacteria in Continuous Ambulatory Peritoneal Dialysis Culture Fluids from Patients with Peritonitis by Using Broad-Range PCR

Author

Kim, Si Hyun, primary, Jeong, Haeng Soon, additional, Kim, Yeong Hoon, additional, Song, Sae Am, additional, Lee, Ja Young, additional, Oh, Seung Hwan, additional, Kim, Hye Ran, additional, Lee, Jeong Nyeo, additional, Kho, Weon-Gyu, additional, and Shin, Jeong Hwan, additional

Published
2012
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47. A novel A allele with c.467C > T and c.784G > A nucleotide substitutions in Korean individuals.

Author

Kim, Hye Ran, Shin, Jae Gyun, Song, Sae Am, Lee, Ja Young, and Oh, Seung Hwan

Subjects
ABO blood group system, ALLELES, AMINO acids, COMPARATIVE studies, GENEALOGY, GENETIC polymorphisms, GENETIC techniques, HISTOCOMPATIBILITY testing, RESEARCH methodology, MEDICAL cooperation, RESEARCH, SERODIAGNOSIS, HLA-B27 antigen, EVALUATION research, GENOTYPES, BLOOD
Abstract

The ABO blood group is the most important blood group system, and accurate ABO typing is essential for safe transfusion and transplantation. The novel I A i allele showed nucleotide I c.467C > T i and I c.784G > A i substitutions in the background of the I A1.01 i allele, resulting in p.Pro156Leu and p.Asp262Asn amino acid substitutions. The nucleotide polymorphism in Exons 6 and 7 of the novel allele introduced in this study compared to other ABO alleles. [Extracted from the article]

Published
2020
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48. Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system.

Author

Kim, Si Hyun, Shin, Jeong Hwan, Mok, Jeong Ha, Kim, Shine Young, Song, Sae Am, Kim, Hye Ran, Kook, Joong-Ki, Chang, Young-Hyo, Bae, Il Kwon, and Lee, Kwangha

Abstract

Introduction. The aim of this study was to differentiate between Candida famata and Candida guilliermondii correctly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. Methods. Twenty-eight Candida strains from blood cultures that had been identified as C. famata (N = 25), C. famata/C. guilliermondii (N = 2), and C. guilliermondii (N = 1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA and ITS genes and compared the results with those obtained by the VITEK 2 system. Results. All 28 isolates were finally identified as C. guilliermondii. Sequencing analysis of the 28S rRNA gene showed 99.80%-100% similarity with C. guilliermondii for all 28 strains. The ITS gene sequencing of the strains showed 98.34%-100% homology with C. guilliermondii. By MALDI-TOF, we could correctly identify 21 (75%) of 28 C. guilliermondii isolates. Conclusion. We should suspect misidentification when C. famata is reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported. [ABSTRACT FROM AUTHOR]

Published
2014
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49. Genotype Analysis of the JR Blood Group in an East Asian Population Using Tetra-Primer ARMS-PCR.

Author

Song SA, Shin JG, and Oh SH

Subjects
Humans, Alleles, DNA Primers genetics, Neoplasm Proteins genetics, Republic of Korea, Female, East Asian People, Blood Group Antigens genetics, Gene Frequency genetics, Polymerase Chain Reaction methods, Genotype, Asian People genetics, ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics
Abstract

Objective: The JR blood group system, officially designated ISBT JR 032, consists of a single antigen called Jr a . This is a high frequency antigen in most populations. The Jr(a-) phenotype is more prevalent in Japanese and Asian populations. Individuals with the Jr(a-) blood type can be recognized incidentally by the production of anti-Jr(a) antibodies and verified by the existence of two null ABCG2 alleles., Methods: We used direct sequencing to analyze the genotype frequency of the ABCG2 null allele ( c.376C>T, rs72552713 ) and compared it with East Asian genomic databases. We developed tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), which is a simple, precise method for determining an individual's genotype and suitable for clinical use, and analyzed a cohort of 300 healthy Koreans., Results: Using direct sequencing, we found that 14 individuals in the cohort carried a heterozygous ABCG2 null allele. We optimized the ARMS-PCR technique to detect and identify this null allele precisely. We identified the presence of this null allele in a heterozygous state using ARMS-PCR., Conclusion: The minor allele frequency of the ABCG2 null allele in the Korean cohort was 2.3%. The estimated genotype frequencies of homozygotes and heterozygotes for this null allele are 0.05% and 4.56%, respectively. The newly developed ARMS-PCR assay would be useful for determining the Jr(a-) antigen status in patients who produce anti-Jr(a) antibodies as well as for selecting Jr(a-) blood donors., (© 2024 by the Association of Clinical Scientists, Inc.)

Published
2024

50. Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae Causing Invasive Pneumococcal Disease in Korea Between 2017 and 2019 After Introduction of the 13-Valent Pneumococcal Conjugate Vaccine.

Author

Kim GR, Kim EY, Kim SH, Lee HK, Lee J, Shin JH, Kim YR, Song SA, Jeong J, Uh Y, Kim YK, Yong D, Kim HS, Kim S, Kim YA, Shin KS, Jeong SH, Ryoo N, and Shin JH

Subjects
Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial, Humans, Microbial Sensitivity Tests, Pneumococcal Vaccines pharmacology, Serogroup, Serotyping, Vaccines, Conjugate pharmacology, Pneumococcal Infections epidemiology, Streptococcus pneumoniae genetics
Abstract

Background: Streptococcus pneumoniae is a serious pathogen causing various infections in humans. We evaluated the serotype distribution and antimicrobial resistance of S. pneumoniae causing invasive pneumococcal disease (IPD) after introduction of pneumococcal conjugate vaccine (PCV)13 in Korea and investigated the epidemiological characteristics of multidrug-resistant (MDR) isolates., Methods: S. pneumoniae isolates causing IPD were collected from 16 hospitals in Korea between 2017 and 2019. Serotyping was performed using modified sequential multiplex PCR and the Quellung reaction. Antimicrobial susceptibility tests were performed using the broth microdilution method. Multilocus sequence typing was performed on MDR isolates for epidemiological investigations., Results: Among the 411 S. pneumoniae isolates analyzed, the most prevalent serotype was 3 (12.2%), followed by 10A (9.5%), 34 (7.3%), 19A (6.8%), 23A (6.3%), 22F (6.1%), 35B (5.8%), 11A (5.1%), and others (40.9%). The coverage rates of PCV7, PCV10, PCV13, and pneumococcal polysaccharide vaccine (PPSV)23 were 7.8%, 7.8%, 28.7%, and 59.4%, respectively. Resistance rates to penicillin, ceftriaxone, erythromycin, and levofloxacin were 13.1%, 9.2%, 80.3%, and 4.1%, respectively. MDR isolates accounted for 23.4% of all isolates. Serotypes 23A, 11A, 19A, and 15B accounted for the highest proportions of total isolates at 18.8%, 16.7%, 14.6%, and 8.3%, respectively. Sequence type (ST)166 (43.8%) and ST320 (12.5%) were common among MDR isolates., Conclusions: Non-PCV13 serotypes are increasing among invasive S. pneumoniae strains causing IPD. Differences in antimicrobial resistance were found according to the specific serotype. Continuous monitoring of serotypes and antimicrobial resistance is necessary for the appropriate management of S. pneumoniae infections.

Published
2023
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