Search

Your search keyword '"Song, Da-feng"' showing total 14 results
Start Over Author "Song, Da-feng"
14 results on '"Song, Da-feng"'

12. Comparative genomic analysis of Lactobacillus plantarumZJ316 reveals its genetic adaptation and potential probiotic profiles

Author

Li, Ping, Li, Xuan, Gu, Qing, Lou, Xiu-yu, Zhang, Xiao-mei, Song, Da-feng, and Zhang, Chen

Abstract

In previous studies, Lactobacillus plantarumZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. The genome sequence of L. plantarumZJ316 was compared with those of eight L. plantarumstrains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. Comparative characterization of the L. plantarumZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate. 前期研究发现植物乳杆菌ZJ316 能显著抑制病原菌,促进仔猪生长,提高猪肉质量等,本研究拟在ZJ316 全基因组测序的基础上,运用比较基因组学手段揭示与其生境适应性及益生特性相关基因。 首次从基因水平上分析与植物乳杆菌ZJ316 的生境适应性、抑菌活性及益生特性等相关的基因,为进一步揭示其生理功能打下基础。 运用BLASTN、Mauve 和MUMmer 等将植物乳杆菌ZJ316 全基因组序列与已测序的8 个植物乳杆菌全基因组序列进行比对及分析; 用CRISPRFinder 寻找CRISPR 重复序列。 植物乳杆菌ZJ316 包含碳水化合物的运输和代谢、蛋白水解酶系统和氨基酸的生物合成等相关基因,具有CRISPR、应激反应、耐胆盐、粘附宿主肠壁、胞外多糖、生物合成和细菌素生物合成等相关基因。这些基因的功能是其作为益生菌的重要特征和基础。

Published
2016
Full Text
View/download PDF

13. Purification and Characterization of Plantaricin ZJ5, a New Bacteriocin Produced by Lactobacillus plantarum ZJ5.

Author

Song, Da-Feng, Zhu, Mu-Yuan, and Gu, Qing

Subjects
*BACTERIOCINS, *LACTOBACILLUS plantarum, *AMMONIUM sulfate, *MOLECULAR weights, *NUCLEOTIDE sequencing, *HOMOLOGY (Biology)
Abstract

The aim of this study is to investigate the antimicrobial potential of Lactobacillus plantarum ZJ5, a strain isolated from fermented mustard with a broad range of inhibitory activity against both Gram-positive and Gram-negative bacteria. Here we present the peptide plantaricin ZJ5 (PZJ5), which is an extreme pH and heat-stable. However, it can be digested by pepsin and proteinase K. This peptide has strong activity against Staphylococcus aureus. PZJ5 has been purified using a multi-step process, including ammonium sulfate precipitation, cation-exchange chromatography, hydrophobic interactions and reverse-phase chromatography. The molecular mass of the peptide was found to be 2572.9 Da using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The primary structure of this peptide was determined using amino acid sequencing and DNA sequencing, and these analyses revealed that the DNA sequence translated as a 44-residue precursor containing a 22-amino-acid N-terminal extension that was of the double-glycine type. The bacteriocin sequence exhibited no homology with known bacteriocins when compared with those available in the database, indicating that it was a new class IId bacteriocin. PZJ5 from a food-borne strain may be useful as a promising probiotic candidate. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

14. [Effects of cu2+ on biosynthesis of camptothecin in cell cultures of Camptotheca acuminata].

Author

Gu Q, Song DF, Zhang H, and Zhu MY

Subjects
Anthocyanins biosynthesis, Camptotheca growth & development, Light, Antineoplastic Agents, Phytogenic biosynthesis, Camptotheca metabolism, Camptothecin biosynthesis, Copper pharmacology
Abstract

Camptothecin is a strong anti-tumor compound isolated from Camptotheca acuminata. One of the most important way for the production of Camptothecin is by cell cultures of Camptotheca acuminata. The effect of Cu2+ on camptothecin accumulation in Camptotheca acuminata cell line was described in this paper. The results showed that the optimum CuCl2 concentration in B5 medium was 0.008 mg/mL, which increased camptothecin production for 30 times compare to the control while has no inhibitive effects on cell growth, at the same time, the peroxidase activity was increased and the anthocyanidin accumulation was inhibited. The promotive effects of Cu2+ on camptothecin accumulation in light was higher than that in dark.

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results