Comparative genomic analysis of Lactobacillus plantarumZJ316 reveals its genetic adaptation and potential probiotic profiles

In previous studies, Lactobacillus plantarumZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. The genome sequence of L. plantarumZJ316 was compared with those of eight L. plantarumstrains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. Comparative characterization of the L. plantarumZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate. 前期研究发现植物乳杆菌ZJ316 能显著抑制病原菌,促进仔猪生长,提高猪肉质量等,本研究拟在ZJ316 全基因组测序的基础上,运用比较基因组学手段揭示与其生境适应性及益生特性相关基因。 首次从基因水平上分析与植物乳杆菌ZJ316 的生境适应性、抑菌活性及益生特性等相关的基因,为进一步揭示其生理功能打下基础。 运用BLASTN、Mauve 和MUMmer 等将植物乳杆菌ZJ316 全基因组序列与已测序的8 个植物乳杆菌全基因组序列进行比对及分析; 用CRISPRFinder 寻找CRISPR 重复序列。 植物乳杆菌ZJ316 包含碳水化合物的运输和代谢、蛋白水解酶系统和氨基酸的生物合成等相关基因,具有CRISPR、应激反应、耐胆盐、粘附宿主肠壁、胞外多糖、生物合成和细菌素生物合成等相关基因。这些基因的功能是其作为益生菌的重要特征和基础。