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IntroductionShort-track speed skating (short track) is an Olympic sport characterized by a specific body position and counterclockwise movement on the track. Based on previous studies, we hypothesized that athlete body positions employed in this sport could lead to asymmetric overuse disorders of the left lower limb muscles. An increased number of latent trigger points (LTrPs) was confirmed in individual muscles of the overloaded left limb of short-track athletes. This study aimed to compare the number of LTrPs and the level of resting muscle tone between elite junior short-track athletes and healthy non-athletes.MethodsThe experimental (EXP) group comprised 15 elite short-track junior athletes from the Polish national team and the control (CON) group comprised 15 healthy young volunteers. In both groups, the left leg was tested for (i) the presence of LTrPs and (ii) resting muscle tone (RMT), assessed using surface electromyography in six muscles.ResultsThe EXP group showed a higher number of LTrPs in the left lower limb, compared with the CON group. The muscle that was most significantly affected in the athletes was the vastus lateralis obliquus [χ2 (1, N = 30), p

PURPOSE: This study explores the relationship between the resilience of the hotel industry (an underdeveloped concept) and the factors that support the development of this industry. It examines whether the resilience of the hotel industry varies regionally and whether it is influenced either by the characteristics of the hotel industry or by regional economic conditions. The study focuses on the COVID-19 pandemic, which particularly hit the hotel industry. METHODOLOGY: The clustering method is used to identify regions in Poland with similar pre-pandemic hotel industry development are identified. Secondly, Potthoff’s analysis and Kruskal-Wallis tests with Dunn’s tests are used respectively to identify variations in the resilience responses of the industry in different regions and to examine the antecedents of these variations. FINDINGS: The data collected indicate variations between clusters in terms of resilience response. Not all factors that support the development of the hotel industry in a particular region are equally conducive to its resilience. Our study shows that the pandemic was not an isolated or unique event but rather a catalyst that brought long-standing issues for the hotel and tourism sector. IMPLICATIONS FOR THEORY AND PRACTICE: This study contributes to the development of resilience theory by providing evidence-based arguments for separating the domains of resilience and development and for a more granular exploration of the trade-offs between them. From the perspective of the hotel industry, insights into narrowly channeled agglomeration externalities during a crisis could inform strategic decisions regarding the location of hotel investments, the value proposition created, and business model diversification. Therefore, there is a need for more resilient crisis management strategies that can be applied across the tourism sector. These strategies should encompass effective supply chain management and a robust framework for labor security. ORIGINALITY AND VALUE: The findings suggest that hotel industry development factors, which include both industry characteristics and regionally shaped economic conditions, provide mixed support for the resilience of this industry. The study revealed clear tensions between development and resilience impacts by highlighting existing trade-offs. Focusing attention on trade-off tensions advances both the conceptual validity and application potential. Although the present study was defined for the specific circumstances of the hotel industry, this approach can be replicated in different industries that are components of the supply chain of the tourism market and its value chain.

Abstract Background Short-track speed skating movement involves asymmetric overloading of the lower left side of the body. The gluteus maximus fatigue limits the physical and mental athletic capacity to perform set tasks. A possible link between the presence of latent trigger points (LTrPs) and muscle fatigue development/persistence has been posited. The aim of the study was to determine whether elite short-track speed skating can result in the impairment of the musculoskeletal system of the lower limbs. Methods Elite short-track athletes as the experimental group (EXP) = 9, 19.5 ± 1.8 years, and healthy subjects as the control group (CON) = 18, 20.8 ± 1.2 years, were tested for: (i) lower limb loading asymmetry using ground reaction force (GRF) measurements during quiet standing, (ii) gluteus maximus fatigue measured with surface electromyography (sEMG) during the Biering-Sorensen test, and (iii) LTrPs presence in the 14 examined muscles of the pelvic girdle and lower limbs. Results There were between-group differences in the number of LTrPs, with the EXP group (left lower limb (LLL) n = 18, right lower limb (RLL) n = 9) showing more LTrPs compared to the CON group (LLL n = 2, RLL n = 1), (p

Herpes simplex virus type 2 (HSV-2) is a sexually transmitted pathogen that causes a persistent infection in sensory ganglia. The infection manifests itself as genital herpes but in rare cases it can cause meningitis. In this study, we used a murine model of HSV-2 meningitis to show that Fas and FasL are induced within the CNS upon HSV-2 infection, both on resident microglia and astrocytes and on infiltrating monocytes and lymphocytes. Mice lacking Fas or FasL had a more severe disease development with significantly higher morbidity, mortality, and an overall higher CNS viral load. In parallel, these Fas/FasL-deficient mice showed a severely impaired infection-induced CNS inflammatory response with lower levels of infiltrating CD4+ T-cells, lower levels of Th1 cytokines and chemokines, and a shift in the balance between M1 and M2 microglia/monocytes. In vitro, we confirmed that Fas and FasL is required for the induction of leucocyte apoptosis, but also show that the Fas/FasL pathway is required for adequate cytokine and chemokine production by glial cells. In summary, our data show that the Fas/FasL cell death receptor pathway is an important defense mechanism in the spinal cord as it down-regulates HSV-2-induced inflammation while at the same time promoting adequate anti-viral immune responses against infection.

Theoretical background: The Regional Comprehensive Economic Partnership (RCEP) is the largest mega-regional trade agreement ever signed, accounting for more than 30% of the global population, exports, and gross domestic product (GDP). It is also the largest automotive market worldwide, with significant potential both in terms of automotive manufacturing and sales. Purpose of the article: The paper’s main objective is to characterize China-RCEP automotive trade in the years 2012–2021 with reference to key provisions and implications of the aforementioned mega-regional trade agreement for the automotive industry. In the empirical part of the paper, the authors attempt to verify the hypothesis that trade liberalization under RCEP may contribute to the expansion of the regional automotive industry in terms of trade volume, as well as fragmentation of value and supply chains. Research methods: The authors conducted an analysis of intra-industry trade disaggregated into 54 six-digit HS tariff codes using the Grubel–Lloyd and Balassa indexes, accompanied by cross sectional analysis and studies of sectoral reports and literature in the field of mega-regionalism in trade. Main findings: The hypothesis formulated in the introduction of the paper was verified positively –the authors provided an added value to previous empirical studies on RCEP, in the form of a perspective of the automotive industry and in-depth analysis of sectoral trends for the last ten years. The potential for further fragmentation of value and supply chains inside the region was identified, including the possibility of relocation of manufacturing and assembly activities to lower the cost of RCEP Member States to strengthen comparative advantages over extra-regional counterparts (EU, USMCA). The authors pointed at the prospective rising attractiveness of RCEP in automotive manufacturers’ location decisions worldwide, considering both costs, retreat from global-oriented manufacturing and export strategies, investment security, sustainable development, as well as geopolitical trends in respect of newly established tariff and non-tariff barriers.

There is an urgent need for novel strategies to combat life-threatening fungal infections. Here, Revie et al. identify a compound that induces alterations in fungal membranes, synergizes with azole antifungals against the pathogen Candida albicans, and inhibits virulence traits and biofilm formation in animal models of infection.

The article presents data on the use in the cheese-making technology starter culture “Enteroplan”, which includes strains of lactic acid bacteria isolated from traditional Carpathian cheese, particularly L. lactis, Lb. plantarum, E. durans in the ratio 50 : 40 : 10. The microflora of traditional dairy products, particularly cheese, has evolved through time and is unique. It is essential to reproduce and preserve such a microbiome in cheese making. A change in the technology was the use of cow's milk by introducing our starter culture, “Enteroplan”. For the manufacturing of bryndza, cow's milk was chosen, which satisfied regulatory documentation criteria in terms of organoleptic characteristics, physicochemical properties, and cheese suitability. According to organoleptic parameters, the manufactured prototype of cheese with starter culture “Enteroplan” had a pronounced, rich creamy-cheese taste and smell; its consistency was homogeneous, plastic, moderately dense, whereas the control cheese made with RSF had a less pronounced taste and smell, and its consistency was homogeneous, plastic, and moderately fragile. No significant discrepancies were found in other organoleptic indicators. According to the results of the scoring of the cheese, the prototype received 3 points more. The taste, odor, and consistency of the test sample were examined above compared to the control sample. During the 20-day maturation period of the cheese, the number of lactic acid bacteria was relatively high, ranging in the control sample from (6.6 ± 0.28)×105 to (2.5 ± 0.23)×105 CFU/g and in the experimental sample from (8.1 ± 0.30)×105 to (6.1 ± 0.18)×105 CFU/g. Pathogenic microflora, mold, and yeast-like fungi were not detected during the storage of the manufactured cheese samples.

With the growing awareness among entrepreneurs and consumers within the tourism industry regarding the field of sustainable development, it seems necessary to base hotel classification systems on an integrated approach to management quality, including the fulfillment of ecological and environmental criteria. However, this topic has yet to be recognized in the literature, especially concerning the European market. Therefore, this article aims to fill this research gap and indicate how environmental standards have been implemented in the European system of hotel classification—Hotelstars Union (HSU)—and that in force in Poland. With regard to these systems, the differences in the classification criteria taken into account by the certifying organizations in the European Union (EU) and the administrative hotel categorization system in Poland are pointed out. Particular emphasis is placed on the criteria related to environmental management in the hotel industry. The criteria related to environmental management were identified through an analysis of the criteria of eight environmental management systems that are applicable to the contemporary hotel industry. The results of this research prove that, even though the HSU system takes the principles of sustainable development into account to a much greater extent than the Polish system, its primary weakness is that most of its criteria related to the environment are optional. In addition, the optional criteria have higher weights than the obligatory ones. In turn, the Polish classification system makes very little reference to environmental standards. Moreover, it is not detailed, and the assessment criteria are not up to date. Therefore, it is recommended that a greater number of environmental criteria be included in the next update of the HSU system, which should be obligatory for hotels to meet. In addition, the sustainability criteria included in the HSU system should be adapted to the policy system aimed at the hotel industry in Poland and other EU countries. [ABSTRACT FROM AUTHOR]

Herpes simplex virus type 2 (HSV-2) is a sexually transmitted pathogen that causes a persistent infection in sensory ganglia. The infection manifests itself as genital herpes but in rare cases it can cause meningitis. In this study, we used a murine model of HSV-2 meningitis to show that Fas and FasL are induced within the CNS upon HSV-2 infection, both on resident microglia and astrocytes and on infiltrating monocytes and lymphocytes. Mice lacking Fas or FasL had a more severe disease development with significantly higher morbidity, mortality, and an overall higher CNS viral load. In parallel, these Fas/FasL-deficient mice showed a severely impaired infection-induced CNS inflammatory response with lower levels of infiltrating CD4+ T-cells, lower levels of Th1 cytokines and chemokines, and a shift in the balance between M1 and M2 microglia/monocytes. In vitro, we confirmed that Fas and FasL is required for the induction of leucocyte apoptosis, but also show that the Fas/FasL pathway is required for adequate cytokine and chemokine production by glial cells. In summary, our data show that the Fas/FasL cell death receptor pathway is an important defense mechanism in the spinal cord as it down-regulates HSV-2-induced inflammation while at the same time promoting adequate anti-viral immune responses against infection. [ABSTRACT FROM AUTHOR]

The global rise in issues related to the invasion of alien tree species is becoming increasingly prominent. Genus Acacia is one of the most pervasive invaders among the non-native flora introduced to Portugal over the centuries. This research aimed to pinpoint the key players engaged in its management, their expertise, and the methodologies applied to control acacias or decrease affected regions. Data were gathered via an online survey and subjected to diverse statistical analyses. Numerous stakeholders have emerged in recent decades, employing various methods to fight acacia invasion. Mechanical, chemical, and combined methods are the most frequently applied. The findings highlight the need for continued funding for long-term acacia control in the same invaded areas, increased training actions and best practices demonstration in the field and increased public awareness and engagement in local communities and the general public.

Many studies have demonstrated a general decline and dysregulation in immune functions with age. It is not clear, however, how the aging affects the immune surveillance of the female reproductive tract (FRT) by γδ T cells, a unique population of T lymphocytes that was shown to regulate homeostasis of epithelial barriers. First, we analyzed γδ T cell presence in FRT in young (2 months) and old (18 months) wild-type (WT) C57BL/6 mice. We did not detect any changes in γδ T cell number nor distribution in the vaginas between the age groups, while in uteri, there was a twofold increase in γδ T cell number in aged mice. To check if γδ T lymphocytes regulate a metabolic and immune status of aging vaginal tissue, we compared the expression of 84 aging-associated genes in young and old WT and γδ T-cell-deficient (Tcrd-/-) mice. We discovered that only the Ltf (lactotransferrin) gene was downregulated in old Tcrd-/- mice. In both mouse strains, we found similar age-dependent changes in cytokine production upon vaginal inflammation due to Toll-like receptor 9 (TLR9) stimulation with CpG. With age in the vaginas, IL-1α and IL-17A levels increased while IL-6, IL-10, MCP-1, and IFNγ levels were diminished in response to CpG. Similar trends were observed in uteri. Interestingly, under the inflammatory state, the lack of γδ T cells in young individuals enhanced MCP-1 production in the vagina and decreased MCP-1 level in the uterus in old females. Our gene expression data point to an antimicrobial role of γδ T lymphocytes. The profile of secreted inflammatory cytokines shifted during aging toward the proinflammatory type, and γδ T cells played a modest fine-tuning role in immunoregulation in aged FRT. We believe this work expands our understanding of γδ T cell functions and the inflammaging in the murine reproductive epithelia.

Formulations of yogurt flavored with coffee with a sugar content of 4 and 6 % and instant coffee Nescafe Gold in the amount of 0.5, 0.7 or 0.9 % were developed. The possibility of including in the formulation of low-fat yogurts protein enrichments, namely dry whey protein concentrate WPC 80 Milkiland in the amount of 1.5 %. A five-point scale has been developed to assess the organoleptic characteristics of new types of yogurt. It was found that the score of the consistency of the samples differed and decreased with increasing coffee content from 0.5 to 0.9 %. The sugar content did not affect the consistency, but higher taste scores were obtained with coffee-flavored samples with the addition of 5 % sugar compared to 4 %. Yogurts with 0.7 % coffee and 4 % sugar and both yogurts with 0.9 % coffee had the lowest scores compared to other yogurt samples. Changes in titratable acidity during yogurt fermentation and during storage were studied. It was found that the highest rate of increase of titratable acidity was registered for sample 3, which at 4 h of fermentation reached 98 °T compared to others, which is explained by the content of 2.2–4.5 % chlorogenic acid and other organic acids in instant coffee. It was found that lactic acid accumulated during storage of yogurts with coffee. On the first day of storage, its content ranged from 0.135–0.200 %, and on the 15th day increased to 1.22–1.42 %. Such changes are natural, because lactic acid is a product of lactic acid microflora metabolism during lactose fermentation. It was investigated that the active acidity at the end of fermentation was 4.56 units. pH against control – 4.72 units. pH and had a higher rate of decline compared to control. This can be explained by the mass fraction of lactose, which in the whey protein concentrate is 36 %. Also, this protein enrichment contains a large number of carboxyl groups, which increases the acidity of the clot and the finished product. All test samples were found to have better rheological properties due to the use of 1.5 % whey protein concentrate and sugar in the formulations. The parameters of technological operations in the production of yogurt with coffee are substantiated.

Glomerular filtration rates for individual kidneys can be measured semi-quantitatively by a gamma camera using [99mTc]Tc-DTPA, with limited diagnostic accuracy. A more precise measurement can be performed on a PET/CT scanner using the radiotracer [68Ga]Ga-EDTA, which has been validated in animal studies. The purpose of this study was to develop an easy kit-based synthesis of [68Ga]Ga-EDTA that is compliant with good manufacturing practice (GMP) and applicable for human use. The production of the cold kit and its labeling were validated, as were the radiochemical purity measurement and analytical procedures for determining the Na2EDTA dihydrate content in the kits. In this study, we validated a GMP kit for the simple production of [68Ga]Ga-EDTA, with the intention of applicability for human use.

The purpose of this article is to define the role and importance of human beings and their weaknesses in the information security system of an organization. All people make mistakes that can sometimes have serious consequences. The working hypothesis presented in this article was expressed in the belief that human beings are the weakest link in the organization's information security. According to the "2014 Cyber Security Intelligence Index", as much as 95% of all security incidents are related to human errors. For organizations, such situations are usually very costly, as they concern people who have access to the most sensitive data. As the study cited in the article shows, the awareness of threats in information systems among the respondents is low. The ongoing changes in the availability of information are associated with the need to constantly improvement its protection. Not only systemic, but also protection resulting from human competences. That is why it is worth focusing on him and considering possible methods of expanding his competences and continuous learning.

Introduction: Short-track speed skating (short track) is an Olympic sport characterized by a specific body position and counterclockwise movement on the track. Based on previous studies, we hypothesized that athlete body positions employed in this sport could lead to asymmetric overuse disorders of the left lower limb muscles. An increased number of latent trigger points (LTrPs) was confirmed in individual muscles of the overloaded left limb of short-track athletes. This study aimed to compare the number of LTrPs and the level of resting muscle tone between elite junior short-track athletes and healthy non-athletes. Methods: The experimental (EXP) group comprised 15 elite short-track junior athletes from the Polish national team and the control (CON) group comprised 15 healthy young volunteers. In both groups, the left leg was tested for (i) the presence of LTrPs and (ii) resting muscle tone (RMT), assessed using surface electromyography in six muscles. Results: The EXP group showed a higher number of LTrPs in the left lower limb, compared with the CON group. The muscle that was most significantly affected in the athletes was the vastus lateralis obliquus [χ² (1, N = 30), p <0.001, V Cramer = 0.71]. This muscle also differed significantly between the groups in terms of the RMT (p = 0.033, Cohen's d = 0.87). Conclusions: Elite short-track junior athletes presented with increased RMT and an increased number of LTrPs in the vastus lateralis oblique muscle, compared with healthy non-athletes. [ABSTRACT FROM AUTHOR]

PURPOSE: This study explores the relationship between the resilience of the hotel industry (an underdeveloped concept) and the factors that support the development of this industry. It examines whether the resilience of the hotel industry varies regionally and whether it is influenced either by the characteristics of the hotel industry or by regional economic conditions. The study focuses on the COVID-19 pandemic, which particularly hit the hotel industry. METHODOLOGY: The clustering method is used to identify regions in Poland with similar pre-pandemic hotel industry development are identified. Secondly, Potthoff's analysis and Kruskal-Wallis tests with Dunn's tests are used respectively to identify variations in the resilience responses of the industry in different regions and to examine the antecedents of these variations. FINDINGS: The data collected indicate variations between clusters in terms of resilience response. Not all factors that support the development of the hotel industry in a particular region are equally conducive to its resilience. Our study shows that the pandemic was not an isolated or unique event but rather a catalyst that brought long-standing issues for the hotel and tourism sector. IMPLICATIONS FOR THEORY AND PRACTICE: This study contributes to the development of resilience theory by providing evidence-based arguments for separating the domains of resilience and development and for a more granular exploration of the trade-offs between them. From the perspective of the hotel industry, insights into narrowly channeled agglomeration externalities during a crisis could inform strategic decisions regarding the location of hotel investments, the value proposition created, and business model diversification. Therefore, there is a need for more resilient crisis management strategies that can be applied across the tourism sector. These strategies should encompass effective supply chain management and a robust framework for labor security. ORIGINALITY AND VALUE: The findings suggest that hotel industry development factors, which include both industry characteristics and regionally shaped economic conditions, provide mixed support for the resilience of this industry. The study revealed clear tensions between development and resilience impacts by highlighting existing trade-offs. Focusing attention on trade-off tensions advances both the conceptual validity and application potential. Although the present study was defined for the specific circumstances of the hotel industry, this approach can be replicated in different industries that are components of the supply chain of the tourism market and its value chain.. [ABSTRACT FROM AUTHOR]

The aim of the work was to improve the technology of melt butter with turmeric and to study the quality indicators of the finished product. The experiments were conducted in the laboratory of the Department of Milk and Dairy Products Technology of Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv. The study was conducted in two stages. At the first stage, a natural source of antioxidants for melt butter was selected, the optimal amount for application was selected and the technology of melt butter with turmeric was improved. In the second stage of research, the quality indicators of the finished product were determined. Ways to improve the stability of melt butter during storage are analyzed and the possibility of using vegetable raw materials, namely turmeric powder in melt butter technology, is substantiated. Turmeric contains vitamins K, B, B1, B2, C, D and minerals, including calcium, iron, phosphorus and iodine, as well as essential oils and their components – sabinene, borneol, cigiberren, terpene alcohols, phelander, curcumin and others. components that, even in microscopic quantities, have a good effect on the human body. Modes of technological operations are described. Turmeric powder is added to the fat fraction obtained as a result of separation, which is sent to intermediate baths and incubated for 1–2 hours. at a temperature of 90–95 °C, stirring every 5–7 minutes. This creates a specific taste and aroma of melt butter and reduces the emulsification ability of the system, which improves the efficiency of subsequent separation. The optimal dose of turmeric powder is 0.5 % by weight of the fat fraction. Selected to obtain a homogeneous consistency of the finished product, the cooling temperature is not higher than 14–18 °C. Organoleptic and physicochemical parameters of the finished product are described. Organoleptic characteristics of melt butter during storage (two months) did not change significantly. The acidity of the fatty phase during storage increased and amounted to 1.82–1.93 °K at a maximum allowable value of 2.4 °K, due to the influence of antioxidant properties of turmeric.

Ancient rhetoric provides knowledge and tools to analyze the tools and techniques used in marketing. This assumption is the working hypothesis of this article. The importance of research on the tools of rhetoric (understood as persuasive communication) used in advertisements is indicated by ancient assumptions concerning the goals and applications of this field of science. The intention of the authors is to check whether these tools allow you to identify what can stand out on the market, observe recipients' reactions to specific stimuli and how to manage the rest of the marketing so that the company has a high position on the market.

The results of studies of protein substances of brine cheese from sheep milk with partial replacement of salt with potassium chloride in the amount of 20 and 30 % are presented in the article. Benefits of cheese as a foodstuff are a large number of vitamins B, A, E, it is rich in minerals, including trace elements and essential amino acids. The bacterial preparation RSF-742 in combination with Fresh-Q (Chr. Hansen, Denmark) was used to make the cheese, which adversely affects the development of yeast and mold, thus extending the shelf life of the cheese. Two groups of cheese were made. The first group (without Fresh-Q): K (control) using NaCl; D1 and D2 with 20 % and 30 % replacement of NaCl by KCl, respectively. Second group (with Fresh-Q): CF using NaCl; DF1 and DF2 with 20 and 30 % replacement of NaCl by KCl, respectively. The Kjeldahl method was used to determine the content of total Nitrogen, total soluble Nitrogen, Nitrogen of non-protein soluble nitrogen-containing compounds. The positive effect of salt replacement and the use of biosecurity culture on the course of proteolytic processes has been proved. Nitrogen content of soluble protein substances, which determines the dietary properties of cheese, increased in samples with 20 and 30 % replacement of table salt with potassium chloride. Determination of digestibility of proteins by digestive enzymes in vitro was performed using the basic method Pokrovsky-Ertanov. The essence of the method consists in the sequential effect on the protein of the object under study of the proteinase system and the removal by dialysis of some hydrolysis products to avoid inhibition of the reaction by low molecular weight peptides and free amino acids. Brynza with partial replacement of sodium chloride with potassium chloride is characterized by better digestibility compared to cheese, which is made by traditional technology. The data are confirmed by high rates of digestibility. The test specimens of the breeze according to these indicators fully meet the requirements of the current regulatory documentation.

GVCs have become an inherent component of the economic landscape of East Asia, one of the key pillars of the regional integration processes and division of labour based on fragmentation and specialisation. The main objective of the paper was to describe the impact of COVID-19 pandemic on GVCs in East Asia from the perspective of Japan, using monthly trade statistics for 2020-2021. The paper indicates the short-term disruptive impact on trade statistics with the prospect of improvement, however, under conditions of the gradual phasing out of the pandemic, the effective utilisation of RCEP concessions to boost GVC trade, and with the better coordination of the regional response to future health crises. Statistical analysis is accompanied by critical analysis of literature in the field of research. The paper delivers the current statistical picture of the impact of the COVID-19 pandemic on intra-RCEP trade, accompanied by references to legal and institutional aspects of the response of individual countries to health crisis.(original abstract)

The Fas/FasL pathway plays a key role in immune homeostasis and immune surveillance. In the central nervous system (CNS) Fas/FasL is involved in axonal outgrowth and adult neurogenesis. However, little is known about the role of the Fas/FasL pathway in herpes encephalitis. In this study, we used a neuropathogenic clinical strain of herpes simplex virus type 1 (HSV-1) to explore infection-induced inflammation and immune responses in the mouse brain and the role of Fas/FasL in antiviral CNS immunity. HSV-1 CNS infection induced the infiltration of Fas- FasL-bearing monocytes and T cells in the brain and also to an up-regulation of Fas and FasL expression on resident astrocytes and microglia within infected sites. Upon infection, Fas- and FasL-deficient mice (lpr and gld) were partially protected from encephalitis with a decreased morbidity and mortality compared to WT mice. Fas/FasL deficiency promoted cell-mediated immunity within the CNS. Fas receptor stimulation abrogated HSV-1 induced activation and inflammatory reactions in microglia from WT mice, while lack of Fas or FasL led to a more pronounced activation of monocytes and microglia and also to an enhanced differentiation of these cells into a pro-inflammatory M1 phenotype. Furthermore, the specific immune system was more efficient in Fas- and FasL-deficient mice with significantly higher numbers of infiltrating HSV-1-specific cytotoxic T cells in the brain. Our data indicate that the Fas/FasL pathway leads to excessive neuroinflammation during HSV-1 infection, which is associated with a diminished anti-viral response and an excessive neuroinflammation.

The eruption of permanent teeth in children is a complex physiological process that is important for the development of the dentoalveolar system. The timing of eruption is a key indicator of biological age and prenosological diagnosis of children's health. The need to update research on this topic is due both to changes in society and the importance of modern data for pediatric dentistry and public health. The aim of the study was to determine the regional characteristics of the terms of eruption of permanent teeth in early mixed dentition among children of Odesa. Material and methods. Dental examination was performed on children aged 4 to 8 years of both sexes. The examination was performed according to the generally accepted methodology, using standard dental instruments included in the examination kit. The children were divided into groups according to age and sex. Patients were examined on the basis of age and period of teeth eruption. Results. The results of the study of the eruption of the permanent teeth of the upper and lower jaws in children aged 4.5-8.5 years in Odesa indicate an earlier eruption of the permanent teeth in girls. Thus, in girls the eruption of the first permanent molars began at the age of 4.5 years and ended at the age of 6 years. In boys, the age of onset of first molar eruption was 5 years and the age of completion was 6.5 years. It was found that the timing of the beginning and the end of the eruption of the central permanent incisors of the upper jaw was the same in boys and girls (6 years), but the average duration of eruption was different. The timing of the beginning and end of eruption of the upper lateral incisors was identical in boys and girls. However, differences in the average terms of eruption of permanent upper lateral incisors were obtained. The analysis of the results of the terms of eruption of the central permanent incisors of the lower jaw in boys and girls shows the same terms of beginning of eruption and average terms of eruption of these teeth. However, there were differences in the time of the end of eruption. The beginning of the eruption of the permanent central incisors of the upper jaw was noted earlier (at the age of 6 years) than the data given in the modern dental manuals of Ukraine and was completed at the age of 8 years. The age of the beginning and the end of eruption was one year earlier than the generally accepted data. The data on the time of eruption of the first permanent molars and incisors obtained as a result of the study are necessary for planning programs for prevention of dental caries in children in the period of early replacement dentition. The data obtained as a result of the study indicate the need for further epidemiological studies in other regions of Ukraine in order to establish the current national norms for the eruption of permanent teeth in children. Conclusion. The discrepancy between the dates of eruption of permanent incisors and molars in Odesa children and the standard dates of eruption of teeth was revealed, which indicates the need to clarify them in each region. Gender differences were found - earlier eruption of permanent teeth in girls. The data of the conducted research substantiate the necessity to revise the terms of caries prophylaxis in Odessa children and the necessity to conduct in-depth examination of children with violation of the terms of teeth eruption. [ABSTRACT FROM AUTHOR]

Brynza is a compulsory product of the daily diet of the inhabitants of the Carpathians and a favorite product for many other consumers, but contains from 4 to 7% of salt. The consumption of a large amount of salt can cause many diseases. The current task of the present is to find ways to extend the shelf life of products provided that the content of their kitchen salt is reduced. One of the ways to reduce the content of the salt is the partial replacement of potassium chloride. The aim of the research was to investigate the course of microbiological processes in brine cheese, the traditional technology of which was improved by 20 and 30% replacement of the salt with potassium chloride in brine and using the bioprotective culture, as well as determining the storage time of the cheese. Preparates used by Chr. Hansen: were used to ferment RSF-742 fermentation culture containing 2 strains of mesophilic and 2 strains of thermophilic microflora, Fresh-Q bioprotection culture and enzyme preparation ‑ CHY-MAX. The action of the Fresh-Q bioprotection culture is aimed at inhibiting the development of yeast and mold, which includes L. rhamnosus. There were made 6 samples of cheese from sheep's milk: K – control sample using the kitchen salt (sodium chloride); D1 and D2 – brynza, made of 20 and 30% by substitution of sodium chloride with potassium chloride, respectively; KF – cheese made using sodium chloride and Fresh-Q bioprotection culture; DF1 and DF2 – brynza, made of 20 and 30% sodium chloride replaced by potassium chloride, respectively, using Fresh-Q. It has been established that the use of Fresh-Q bioprotection culture has a greater effect on organoleptic properties than the replacement of the kitchen salt. According to the results of the score, the highest score was obtained with samples using a bioprotective culture. It is proved that the replacement of the kitchen salt and the use of Fresh-Q provides a high quality product and its compliance with the requirements of regulatory documentation. The study of the course of microbiological processes confirms the expediency of improving the traditional technology of the production of brynza by partial replacement of the kitchen salt with potassium chloride and the addition of Fresh-Q. The research results confirm that the cheese storage period is lengthened by 10 days in samples made using Fresh-Q bioprotection culture.

Empirical data as well as scientific reports confirm that startups require diverse, in-house competencies in order to achieve market success. However, while technology-related competencies are usually brought to new tech ventures by their founders, there is a significant deficiency of market- and management-related competencies, which is reflected in the statistics of common startup failure reasons. In this context, a question arises: how interdisciplinarity of knowledge and competencies can be built into the very core of tech startups? The text addresses this question by zeroing in on the role of universities in this process. In the first part, the specificity of tech startups is analyzed. The next chapter overviews the hitherto academy-related startup education environment together with its shortcomings as far as interdisciplinarity is concerned. Finally, the case study of innovative and interdisciplinary academic ecosystem, which was built and tested within the project UniStartApp, is presented and discussed together with related lessons learned. The text is concluded with final remarks on challenges involved in embedding interdisciplinarity into startup education ecosystem.

Celem autorek artykułu było zbadanie, czy działania proekologiczne podejmowane w hotelach są istotne dla ich gości. Aby móc odpowiedzieć na to pytanie zastosowano badania ankietowe, w których wzięło udział 274 respondentów goszczących w 23 hotelach na terenie Polski. Wykazano, że 32,1% respondentów przy wyborze obiektu na nocleg bierze pod uwagę fakt prowadzenia w hotelu działań proekologicznych, a blisko 62% gości uważa, że w obiektach powinny być podejmowane tego typy inicjatywy. Wśród głównych korzyści ankietowani wymieniali: ochronę środowiska, oszczędność kosztów operacyjnych oraz efekty promocyjne i pozytywny wpływ na wizerunek. Ponad 35% gości byłoby w stanie zapłacić więcej za pobyt wiedząc, że w hotelu prowadzone są działania przyjazne środowisku.

The purpose of the research was to learn whether pro-ecological policies are important to guests staying at hotels. It is based on a survey of 274 guests staying at 23 hotels in Poland. Research has shown that 32.1% of survey participants consider proecological issues when choosing a stay at a hotel. Close to 62% of guests believe that hotels should pursue such initiatives which are believed to yield environmental benefits, reductions in hotel operating costs, promotional effects, and a positive impact on a hotel's image. More than 35% of guests would be willing to pay more for a stay at a hotel that pursues environmentally-friendly policies. It is important to emphasize that pro-ecological activity is not used in hotel rankings in Poland.