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129 results on '"Skovorodkin, Ilya"'

5. From ex ovo to in vitro: xenotransplantation and vascularization of mouse embryonic kidneys in a microfluidic chip.

Author

Oliveira, Micaela, Sarker, Partha Protim, Skovorodkin, Ilya, Kalantarifard, Ali, Haskavuk, Tugce, Mac Intyre, Jonatan, Nallukunnel Raju, Elizabath, Nooranian, Samin, Shioda, Hiroki, Nishikawa, Masaki, Sakai, Yasuyuki, Vainio, Seppo J., Elbuken, Caglar, and Raykhel, Irina

Subjects
ORGANS (Anatomy), MORPHOLOGY, LABS on a chip, CHORIOALLANTOIS, BLOOD filtration
Abstract

Organoids are emerging as a powerful tool to investigate complex biological structures in vitro. Vascularization of organoids is crucial to recapitulate the morphology and function of the represented human organ, especially in the case of the kidney, whose primary function of blood filtration is closely associated with blood circulation. Current in vitro microfluidic approaches have only provided initial vascularization of kidney organoids, whereas in vivo transplantation to animal models is problematic due to ethical problems, with the exception of xenotransplantation onto a chicken chorioallantoic membrane (CAM). Although CAM can serve as a good environment for vascularization, it can only be used for a fixed length of time, limited by development of the embryo. Here, we propose a novel lab on a chip design that allows organoids of different origin to be cultured and vascularized on a CAM, as well as to be transferred to in vitro conditions when required. Mouse embryonic kidneys cultured on the CAM showed enhanced vascularization by intrinsic endothelial cells, and made connections with the chicken vasculature, as evidenced by blood flowing through them. After the chips were transferred to in vitro conditions, the vasculature inside the organoids was successfully maintained. To our knowledge, this is the first demonstration of the combination of in vivo and in vitro approaches applied to microfluidic chip design. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis

Author

Ravindran, Aarthi, primary, Holappa, Lari, additional, Niskanen, Henri, additional, Skovorodkin, Ilya, additional, Kaisto, Susanna, additional, Beter, Mustafa, additional, Kiema, Miika, additional, Selvarajan, Ilakya, additional, Nurminen, Valtteri, additional, Aavik, Einari, additional, Aherrahrou, Rédouane, additional, Pasonen-Seppänen, Sanna, additional, Fortino, Vittorio, additional, Laakkonen, Johanna P, additional, Ylä-Herttuala, Seppo, additional, Vainio, Seppo, additional, Örd, Tiit, additional, and Kaikkonen, Minna U, additional

Published
2024
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11. Potential strategies to prevent encrustations on urinary stents and catheters – thinking outside the box: a European network of multidisciplinary research to improve urinary stents (ENIUS) initiative

Author

Abou-Hassan, Ali, primary, Barros, Alexandre, additional, Buchholz, Noor, additional, Carugo, Dario, additional, Clavica, Francesco, additional, de Graaf, Petra, additional, de La Cruz, Julia, additional, Kram, Wolfgang, additional, Mergulhao, Filipe, additional, Reis, Rui L, additional, Skovorodkin, Ilya, additional, Soria, Federico, additional, Vainio, Seppo, additional, and Zheng, Shaokai, additional

Published
2021
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12. Potential strategies to prevent encrustations on urinary stents and catheters–thinking outside the box: a European network of multidisciplinary research to improve urinary stents (ENIUS) initiative

Author

Urologie Onderzoek, Other research (not in main researchprogram), Abou-Hassan, Ali, Barros, Alexandre, Buchholz, Noor, Carugo, Dario, Clavica, Francesco, de Graaf, Petra, de La Cruz, Julia, Kram, Wolfgang, Mergulhao, Filipe, Reis, Rui L., Skovorodkin, Ilya, Soria, Federico, Vainio, Seppo, Zheng, Shaokai, Urologie Onderzoek, Other research (not in main researchprogram), Abou-Hassan, Ali, Barros, Alexandre, Buchholz, Noor, Carugo, Dario, Clavica, Francesco, de Graaf, Petra, de La Cruz, Julia, Kram, Wolfgang, Mergulhao, Filipe, Reis, Rui L., Skovorodkin, Ilya, Soria, Federico, Vainio, Seppo, and Zheng, Shaokai

Published
2021

18. Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Author

Tarakanchikova, Yana, Alzubi, Jamal, Pennucci, Valentina, Follo, Marie, Kochergin, Boris, Muslimov, Albert, Skovorodkin, Ilya, Vainio, Seppo, Antipina, Maria N., Atkin, Vsevolod, Popov, Alexey, Meglinski, Igor, Cathomen, Toni, Cornu, Tatjana I., Gorin, Dmitry A., Sukhorukov, Gleb B., Nazarenko, Irina, Tarakanchikova, Yana, Alzubi, Jamal, Pennucci, Valentina, Follo, Marie, Kochergin, Boris, Muslimov, Albert, Skovorodkin, Ilya, Vainio, Seppo, Antipina, Maria N., Atkin, Vsevolod, Popov, Alexey, Meglinski, Igor, Cathomen, Toni, Cornu, Tatjana I., Gorin, Dmitry A., Sukhorukov, Gleb B., and Nazarenko, Irina

Abstract

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co‐transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

Published
2020

21. 3D bioprinting of the kidney—hype or hope?

Author

Turunen, Sanna, Kaisto, Susanna, Skovorodkin, Ilya, Mironov, Vladimir, Kalpio, Tomi, Vainio, Seppo, Rak-Raszewska, Aleksandra, Tampere University, Faculty of Biomedical Sciences and Engineering, and Research group: Biomaterials and Tissue Engineering Group

Subjects
217 Medical engineering
Abstract

Three-dimensional (3D) bioprinting is an evolving technique that is expected to revolutionize the field of regenerative medicine. Since the organ donation does not meet the demands for transplantable organs, it is important to think of another solution, which may and most likely will be provided by the technology of 3D bioprinting. However, even smaller parts of the printed renal tissue may be of help, e.g. in developing better drugs. Some simple tissues such as cartilage have been printed with success, but a lot of work is still required to successfully 3D bioprint complex organs such as the kidneys. However, few obstacles still persist such as the vascularization and the size of the printed organ. Nevertheless, many pieces of the puzzle are already available and it is just a matter of time to connect them together and 3D bioprint the kidneys. The 3D bioprinting technology provides the precision and fast speed required for generating organs. In this review, we describe the recent developments in the field of developmental biology concerning the kidneys; characterize the bioinks available for printing and suitable for kidney printing; present the existing printers and possible printing strategies. Moreover, we identify the most difficult challenges in printing of the kidneys and propose a solution, which may lead to successful bioprinting of the kidney. publishedVersion

Published
2018

23. Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Author

Tarakanchikova, Yana, primary, Alzubi, Jamal, additional, Pennucci, Valentina, additional, Follo, Marie, additional, Kochergin, Boris, additional, Muslimov, Albert, additional, Skovorodkin, Ilya, additional, Vainio, Seppo, additional, Antipina, Maria N., additional, Atkin, Vsevolod, additional, Popov, Alexey, additional, Meglinski, Igor, additional, Cathomen, Toni, additional, Cornu, Tatjana I., additional, Gorin, Dmitry A., additional, Sukhorukov, Gleb B., additional, and Nazarenko, Irina, additional

Published
2019
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26. Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Author

Avsievich, Tatiana, Tarakanchikova, Yana, Zhu, Ruixue, Popov, Alexey, Bykov, Alexander, Skovorodkin, Ilya, Vainio, Seppo, Meglinski, Igor, Avsievich, Tatiana, Tarakanchikova, Yana, Zhu, Ruixue, Popov, Alexey, Bykov, Alexander, Skovorodkin, Ilya, Vainio, Seppo, and Meglinski, Igor

Abstract

In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

Published
2019

31. Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumourigenesis gene discovery model

Author

Xu, Qi, primary, Junttila, Sanna, additional, Scherer, Andreas, additional, Giri, Khem Raj, additional, Kivelä, Oona, additional, Skovorodkin, Ilya, additional, Röning, Juha, additional, Quaggin, Susan E., additional, Marti, Hans-Peter, additional, Shan, Jingdong, additional, Samoylenko, Anatoly, additional, and Vainio, Seppo J., additional

Published
2017
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32. CD146 + cells are essential for kidney vasculature development

Author

Halt, Kimmo J., primary, Pärssinen, Heikki E., additional, Junttila, Sanna M., additional, Saarela, Ulla, additional, Sims-Lucas, Sunder, additional, Koivunen, Peppi, additional, Myllyharju, Johanna, additional, Quaggin, Susan, additional, Skovorodkin, Ilya N., additional, and Vainio, Seppo J., additional

Published
2016
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37. Titania nanofibers in gypsum composites : an antibacterial and cytotoxicology study

Author

Mohl, Melinda, Aron Dombovari, Aron, Tuchina, Elena S., Petrov, Pavel O., Bibikova, Olga A., Skovorodkin, Ilya, Popov, Alexey P., Rautio, Anne-Riikka, Sarkar, Anjana, Mikkola, Jyri-Pekka, Huuhtanen, Mika, Vainio, Seppo, Keiski, Riitta L., Prilepsky, Arthur, Kukovecz, Akos, Konya, Zoltan, Tuchincmn, Valery V., Kordas, Krisztian, Mohl, Melinda, Aron Dombovari, Aron, Tuchina, Elena S., Petrov, Pavel O., Bibikova, Olga A., Skovorodkin, Ilya, Popov, Alexey P., Rautio, Anne-Riikka, Sarkar, Anjana, Mikkola, Jyri-Pekka, Huuhtanen, Mika, Vainio, Seppo, Keiski, Riitta L., Prilepsky, Arthur, Kukovecz, Akos, Konya, Zoltan, Tuchincmn, Valery V., and Kordas, Krisztian

Abstract

Further developments of antibacterial coatings based on photocatalytic nanomaterials could be a promising route towards potential environmentally friendly applications in households, public buildings and health care facilities. Hereby we describe a simple chemical approach to synthesize photocatalytic nanomaterial-embedded coatings using gypsum as a binder. Various types of TiO2 nanofiber-based photocatalytic materials (nitrogen-doped and/or palladium nanoparticle decorated) and their composites with gypsum were characterized by means of scanning (SEM) and transmission (TEM) electron microscopy as well as electron and X-ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDX) techniques. These gypsum-based composites can be directly applied as commercially available paints on indoor walls. Herein we report that surfaces coated with photocatalytic composites exhibit excellent antimicrobial properties by killing both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) under blue light. In the case of MSSA cells, the palladium nanoparticle-decorated and nitrogen-doped TiO2 composites demonstrated the highest antimicrobial activity. For the MRSA strain even pure gypsum samples were proven to be efficient in eradicating Gram-positive human pathogens. The cytotoxicity of freestanding TiO2 nanofibers was revealed by analyzing the viability of HeLa cells using MTT and fluorescent cell assays.

Published
2014
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38. Study of a rediscovered large freshwater multinucleate amoeba Chaos illinoisense Kudo, 1950)

Author

Goodkov, Andrew, Smirnov, Alexey, and Skovorodkin, Ilya

Abstract

A large freshwater multinucleate amoeba Chaos illinoisense (Amoebidae), previously only recorded in natural collection by Kudo (1950, 1951) from a small pond in Illinois, Central North America, has been rediscovered in backwaters of the River Luga, North-West Russia. Amoebae were cultured, cloned, and studied using lightand electron-microscopic techniques. This species is the only currently known cyst-forming representative of the genus. Numerous cytoplasmic crystals is a conspicuous characteristic of the species; they appear in two forms: platelike and bipyramidal. Trophozoites contain several hundred spherical nuclei of granular type. The fibrous inner nuclear lamina with loosely organised honeycomb-like structure was revealed as well as distinct mitochondrial heteromorphism. Two-layered cell surface coat with filamentous appearance is demonstrated. Current justification of the specific diagnosis is presented.

Published
1999

39. Titania nanofibers in gypsum composites: an antibacterial and cytotoxicology study

Author

Mohl, Melinda, primary, Dombovari, Aron, additional, Tuchina, Elena S., additional, Petrov, Pavel O., additional, Bibikova, Olga A., additional, Skovorodkin, Ilya, additional, Popov, Alexey P., additional, Rautio, Anne-Riikka, additional, Sarkar, Anjana, additional, Mikkola, Jyri-Pekka, additional, Huuhtanen, Mika, additional, Vainio, Seppo, additional, Keiski, Riitta L., additional, Prilepsky, Arthur, additional, Kukovecz, Akos, additional, Konya, Zoltan, additional, Tuchin, Valery V., additional, and Kordas, Krisztian, additional

Published
2014
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40. Plasmon-resonant gold nanoparticles with variable morphology as optical labels and drug carriers for cytological research

Author

Bibikova, Olga, primary, Popov, Alexey, additional, Skovorodkin, Ilya, additional, Prilepskyi, Artur, additional, Pylaev, Timofey, additional, Bykov, Alexander, additional, Staroverov, Sergey, additional, Bogatyrev, Vladimir, additional, Tuchin, Valery, additional, Kinnunen, Matti, additional, Vainio, Seppo, additional, Kordas, Krizstian, additional, and Khlebtsov, Nikolai, additional

Published
2013
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43. A Secreted BMP Antagonist, Cer1, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development

Author

Chi, Lijun, primary, Saarela, Ulla, additional, Railo, Antti, additional, Prunskaite-Hyyryläinen, Renata, additional, Skovorodkin, Ilya, additional, Anthony, Shelagh, additional, Katsu, Kenjiro, additional, Liu, Yu, additional, Shan, Jingdong, additional, Salgueiro, Ana Marisa, additional, Belo, José António, additional, Davies, Jamie, additional, Yokouchi, Yuji, additional, and Vainio, Seppo J., additional

Published
2011
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46. CD146+cells are essential for kidney vasculature development

Author

Halt, Kimmo J., Pärssinen, Heikki E., Junttila, Sanna M., Saarela, Ulla, Sims-Lucas, Sunder, Koivunen, Peppi, Myllyharju, Johanna, Quaggin, Susan, Skovorodkin, Ilya N., and Vainio, Seppo J.

Abstract

The kidney vasculature is critical for renal function, but its developmental assembly mechanisms remain poorly understood and models for studying its assembly dynamics are limited. Here, we tested whether the embryonic kidney contains endothelial cells (ECs) that are heterogeneous with respect to VEGFR2/Flk1/KDR, CD31/PECAM, and CD146/MCAM markers. Tie1Cre;R26RYFP-based fate mapping with a time-lapse in embryonic kidney organ culture successfully depicted the dynamics of kidney vasculature development and the correlation of the process with the CD31+EC network. Depletion of Tie1+or CD31+ECs from embryonic kidneys, with either Tie1Cre-induced diphtheria toxin susceptibility or cell surface marker–based sorting in a novel dissociation and reaggregation technology, illustrated substantial EC network regeneration. Depletion of the CD146+cells abolished this EC regeneration. Fate mapping of green fluorescent protein (GFP)-marked CD146+/CD31-cells indicated that they became CD31+cells, which took part in EC structures with CD31+wild-type ECs. EC network development depends on VEGF signaling, and VEGF and erythropoietin are expressed in the embryonic kidney even in the absence of any external hypoxic stimulus. Thus, the ex vivoembryonic kidney culture models adopted here provided novel ways for targeting renal EC development and demonstrated that CD146+cells are critical for kidney vasculature development.

Published
2016
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48. A Secreted BMP Antagonist, Cer1, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development.

Author

Lijun Chi, Saarela, Ulla, Railo, Antti, Prunskaite-Hyyryläinen, Renata, Skovorodkin, Ilya, Anthony, Shelagh, Katsu, Kenjiro, Yu Liu, Shan, Jingdong, Salgueiro, Ana Marisa, Belo, José Antó nio, Davies, Jamie, Yuji Yokouchi, and Vainio, Seppo J.

Subjects
KIDNEYS, EPITHELIAL cells, LABORATORY mice, TOMOGRAPHY, TREES, EMBRYOS
Abstract

The epithelial ureteric bud is critical for mammalian kidney development as it generates the ureter and the collecting duct system that induces nephrogenesis in dicrete locations in the kidney mesenchyme during its emergence. We show that a secreted Bmp antagonist Cerberus homologue (Cer1) fine tunes the organization of the ureteric tree during organogenesis in the mouse embryo. Both enhanced ureteric expression of Cer1 and Cer1 knock out enlarge kidney size, and these changes are associated with an altered three-dimensional structure of the ureteric tree as revealed by optical projection tomography. Enhanced Cer1 expression changes the ureteric bud branching programme so that more trifid and lateral branches rather than bifid ones develop, as seen in time-lapse organ culture. These changes may be the reasons for the modified spatial arrangement of the ureteric tree in the kidneys of Cer1+ embryos. Cer1 gain of function is associated with moderately elevated expression of Gdnf and Wnt11, which is also induced in the case of Cer1 deficiency, where Bmp4 expression is reduced, indicating the dependence of Bmp expression on Cer1. Cer1 binds at least Bmp2/4 and antagonizes Bmp signalling in cell culture. In line with this, supplementation of Bmp4 restored the ureteric bud tip number, which was reduced by Cer1+ to bring it closer to the normal, consistent with models suggesting that Bmp signalling inhibits ureteric bud development. Genetic reduction of Wnt11 inhibited the Cer1-stimulated kidney development, but Cer1 did not influence Wnt11 signalling in cell culture, although it did inhibit the Wnt3a-induced canonical Top Flash reporter to some extent. We conclude that Cer1 fine tunes the spatial organization of the ureteric tree by coordinating the activities of the growth-promoting ureteric bud signals Gndf and Wnt11 via Bmp-mediated antagonism and to some degree via the canonical Wnt signalling involved in branching. [ABSTRACT FROM AUTHOR]

Published
2011
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49. Co-infection of the Macronucleus of Paramecium caudatum by Free-Living Bacteria Together with the Infectious Holospora obtusa.

Author

Fokin, Sergei O., Skovorodkin, Ilya N., Schweikert, Michael, and Görtz, Hans-D.

Subjects
*CILIATA, *CELL nuclei, *ENDOSYMBIOSIS, *INTRACELLULAR pathogens, *PATHOGENIC microorganisms, *PARAMECIUM caudatum
Abstract

Studies the macronucleus of Paramecium caudata by researchers from Russia and Germany. Performance of infection experiments incubating P. caudata with non-infectious free-living bacteria or weakly infectious intracellular bacteria; Access of the non-infectious free-living bacteria into the nuclei when added to Paramecium together with Holospora obtusa; Evidence that H. obtusa may facilitate entry of other non-infectious bacteria into the nuclei of Paramecium.

Published
2004
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50. a-Tubulin Minichromosome Promoters in the Stichotrichous Ciliate Stylonychia lemnae

Author

Skovorodkin, Ilya, Pimenov, Alexander, Raykhel, Irina, Schimanski, Bernd, Ammermann, Dieter, and Gu¨nzl, Arthur

Abstract

ABSTRACTCiliated protists are model organisms for a number of molecular phenomena including telomerase function, self-splicing introns, and an RNA interference-related mechanism in programmed DNA elimination. Despite this relevance, our knowledge about promoters and transcriptional regulation in these organisms is very limited. The macronuclear genome of stichotrichous ciliates consists of minichromosomes which typically encode a single gene. The 5' nontranscribed spacers are usually no longer than 400 bp and highly suitable for promoter characterizations. We used microinjection of two artificial and differently tagged a1 tubulin minichromosomes into the macronucleus of Stylonychia lemnaeas a means to characterize in detail the corresponding promoter. Clonal cell lines that stably maintained both minichromosomes were generated, enabling comparative expression analysis by primer extension assays. Deletion and block substitution mutations of one of the minichromosomes revealed a TATA-like element, a putative initiator element, and two distinct upstream sequence elements (USEs). Determination of transcription initiation sites and a sequence alignment indicated that both TATA-like and initiator elements are conserved components of S. lemnaeminichromosomes, whereas the USEs appear to be specific for the a1 tubulin minichromosome. The a2 tubulin minichromosome promoter is very short, comprising the two proximal elements but not the USEs. Despite the latter finding, up-regulation of a-tubulin expression in cells treated with concanavalin A activated the a2 but not the a1 tubulin promoter. These results therefore show that gene expression regulation in S. lemnaeoccurs at the level of transcription initiation on the basis of structurally different promoters.

Published
2007
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