172 results on '"Sivakumar, Uthandi"'
Search Results
2. Entomopathogenic potential of indigenous Simplicillium subtropicum (Hypocreales: Cordycipitaceae) isolates from Tamil Nadu, India, against the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae)
- Author
-
Krishnaveni Mariappan, Jeyarajan Nelson Samuel, Nakkeeran Sevugapperumal, Sivakumar Uthandi, Mohankumar Subbarayalu, and Premalatha Krishnamoorthi
- Subjects
Entomopathogenic fungi ,Simplicillium subtropicum ,Aphis gossypii ,Scanning electron microscope (SEM) ,Agriculture - Abstract
Abstract Background Aphis gossypii Glover (Hemiptera: Aphididae) is an economically important polyphagous pest species infesting many agricultural and horticultural crops causing severe yield losses. In order to develop biopesticides for the management of aphids in an environmentally safe manner, 6 indigenous entomopathogenic fungi Simplicillium subtropicum were isolated from soils of Tamil Nadu and their pathogenicity was tested against A. gossypii under laboratory conditions after characterization. Results Phenotypic and molecular characterization was performed for all the isolates for species identification. Results of the pathogenicity test showed that the isolate S6 (PP446637) performed well among other isolates causing 58.33% mortality on 7 days after treatment (DAT) @ 1 × 108 spores/ml. The LC50 and LT50 values were found to be 2.36 × 107 spores/ml at 7 DAT and 160.20 h @ 1 × 108 spores/ml, respectively. SEM images of A. gossypii treated with the isolate S6 (PP446637) revealed that initial phase of infection, i.e., adhesion, formation of germ tube and appressoria starts within 24 h post-infection (hpi), hyphal development and penetration of cuticle occurred within 48 hpi and extrusion of mycelium and conidiogenesis occurred within 72 hpi. Conclusion This study reveals the entomopathogenic potential of indigenous S. subtropicum isolates against A. gossypii and suggests their use for the eco-friendly management of aphids.
- Published
- 2024
- Full Text
- View/download PDF
3. Volatile Formulation of Mentha spicata Induced Defense Mechanism in Tomato and Suppressed Damping Off Disease Caused by Pythium aphanidermatum
- Author
-
Praveen, Thangaraj, Gurudevan, Thiribhuvanamala, Nallusamy, Saranya, Krishnamoorthy, Akkanna Subbiah, Nakkeeran, Sevugapperumal, Sivakumar, Uthandi, Amirtham, Damodarasamy, and Haripriya, Shanmugam
- Published
- 2023
- Full Text
- View/download PDF
4. EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)
- Author
-
Santhoshkumar Subramaniam, Kumutha Karunanandham, A. S. M. Raja, S. K. Shukla, and Sivakumar Uthandi
- Subjects
Cotton spinning mill waste ,EnZolv ,Laccase ,Optimization ,Pretreatment ,Response surface methodology ,Biotechnology ,TP248.13-248.65 ,Fuel ,TP315-360 - Abstract
Abstract Background EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed to evaluate optimal conditions to remove lignin and other impurities from cotton spinning mill waste (CSMW), without compromising the quality and strength of the fibers. CSMW is a low-cost and readily available source of cellulose, making it an ideal candidate for delignification using EnZolv. By optimizing the pretreatment conditions and harnessing the potential of enzymatic delignification, this research aims to contribute to more sustainable and efficient ways of utilizing lignocellulosic biomass in various industries for the production of biochemical and bioproducts. Results The present study emphasizes the EnZolv pretreatment in the delignification of cotton spinning mill wastes irrespective of the cellulose content. EnZolv process parameters such as, moisture content, enzyme load, incubation time, incubation temperature, and shaking speed were optimized. Under pre-optimized conditions, the percent lignin reduction was 61.34%, 61.64%, 41.85%, 35.34%, and 35.83% in blowroom droppings (BD), flat strips (FS), lickerin fly (LF), microdust (MD) and comber noils (CN), respectively. Using response surface methodology (RSM), the statistically optimized EnZolv pretreatment conditions showed lignin reduction of 59.16%, 62.88%, 48.26%, 34.64%, and 45.99% in BD, FS, LF, MD, and CN, respectively. Conclusion Traditional chemical-based pretreatment methods often involve harsh chemicals and high energy consumption, which can have detrimental effects on the environment. In contrast, EnZolv offers a greener approach by utilizing enzymes that are biodegradable and more environmentally friendly. The resulting fibers from EnZolv treatment exhibit improved properties that make them suitable for various applications. Some of the key properties include enhanced cellulose recovery, reduced lignin content, and improved biophysical and structural characteristics. These improvements can contribute to the fiber's performance and processability in different industries and future thrust for the production of cellulose-derived and lignin-derived bioproducts. Graphical Abstract
- Published
- 2024
- Full Text
- View/download PDF
5. Machine learning approaches for estimation of the fraction of absorbed photosynthetically active radiation and net photosynthesis rate of maize using multi-spectral sensor
- Author
-
Pradosh Kumar Parida, Somasundaram Eagan, Krishnan Ramanujam, Radhamani Sengodan, Sivakumar Uthandi, Parameswari Ettiyagounder, and Raja Rajagounder
- Subjects
Precision agriculture ,Multispectral images ,Machine learning ,UAV ,Vegetation indices ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The fraction of absorbed photosynthetically active radiation (FAPAR) and the photosynthesis rate (Pn) of maize canopies were identified as essential photosynthetic parameters for accurately estimating vegetation growth and productivity using multispectral vegetation indices (VIs). Despite their importance, few studies have compared the effectiveness of multispectral imagery and various machine learning techniques in estimating these photosynthetic traits under high vegetation coverage. In this study, seventeen multispectral VIs and four machine learning (ML) algorithms were utilized to determine the most suitable model for estimating maize FAPAR and Pn during the kharif and rabi seasons at Tamil Nadu Agricultural University, Coimbatore, India. Results demonstrate that indices such as OSAVI, SAVI, EVI-2, and MSAVI-2 during the kharif and MNDVIRE and MSRRE during the rabi season outperformed others in estimating FAPAR and Pn values. Among the four ML methods of random forest (RF), extreme gradient boosting (XGBoost), support vector regression (SVR), and multiple linear regression (MLR) considered, RF consistently showed the most effective fitting effect and XGBoost demonstrated the least fitting accuracy for FAPAR and Pn estimation. However, SVR with R2 = 0.873 and RMSE = 0.045 during the kharif and MLR with R2 = 0.838 and RMSE = 0.053 during the rabi season demonstrated higher fitting accuracy, particularly notable for FAPAR prediction. Similarly, in the prediction of Pn, MLR showed higher fitting accuracy with R2 = 0.741 and RMSE = 2.531 during the kharif and R2 = 0.955 and RMSE = 1.070 during the rabi season. This study demonstrated the potential of combining UAV-derived VIs with ML to develop accurate FAPAR and Pn prediction models, overcoming VI saturation in dense vegetation. It underscores the importance of optimizing these models to improve the accuracy of maize vegetation assessments during various growing seasons.
- Published
- 2024
- Full Text
- View/download PDF
6. A root-associated Bacillus albus LRS2 and its metabolites for plant growth promotion and drought stress tolerance in little millet (Panicum sumatrense L.)
- Author
-
Monisha Rajkumar, Shobana Narayanasamy, and Sivakumar Uthandi
- Subjects
Root endophytes ,Bacillus ,Drought stress ,Millet ,Plant growth ,Induced systemic tolerance ,Plant ecology ,QK900-989 - Abstract
The exploitation of drought-tolerant plant-associated bacteria in sustainable agriculture is considered potential for boosting plant growth and development under adverse environments featured by low water potentials. Herein, the present study aimed to decode the drought-tolerant root-associated bacteria from little millet (Panicum sumatrense L.). A sum of 25 bacterial isolates was obtained from roots of little millet (Var. ATL1) grown under induced drought conditions (-10 bars). They were initially assessed for their capacity to tolerate the drought up to – 36.6 bars (-3.6 MPa) on PEG (PEG 6000) infused agar plates and of which, 9 isolates (LRS1, LRS2, LRS6, LRS9, LRS14, LRS17, LRS22, LRS23, LRS25) were selected. Further, the cultures were assessed for their plant growth-promoting (PGP) traits such as the production of 1-aminocyclopropane-1-carboxylate deaminase (ACCd), exopolysaccharide (EPS), siderophore, indole acetic acid (IAA), ammonia, and hydrogen cyanide (HCN) and plant nutrition properties including phosphorous, potassium, and zinc. Of three isolates, LRS2 produced the highest ACCd (147 mol α- ketobutyrate mg protein–1h–1), IAA (15.89 μg mL–1), siderophore (54.85 % ) and P solubilization compared to other isolates. Further, the potential isolates LRS2, LRS22 and LRS23 were identified as Bacillus albus, Bacillus amyloliquefaciens and Enterobacter sp. respectively based on 16S rRNA sequence analysis. Biotization of little millet seeds (ATL1) with these strains showed that the inoculation of B. albus LRS2 elevated the plant germination (33 %), shoot length (13.5 %), and root length (25.2 %) followed by LRS23 with plant germination (22 %), shoot length (12.2 %) and root length (13.2 %) under induced drought stress (-0.45 MPa). Besides, metabolite profiling of B. albus LRS2 under induced stress analyzed in GC–MS yielded several drought-tolerant metabolites belonging to the class viz., organic acids, fatty acids, amino acid and its derivatives, organoheterocyclic compounds, and benzenoids. The compounds responsible for drought tolerance such as phenol, proline, fumaric acid, ascorbic acid, and gibberellic acid are more pronounced under induced drought stress (PEG 6000) which would aid in drought stress tolerance and facilitate plant health and fitness. These results implied that B. albus LRS2, a root-associated drought-tolerant endophytic bacteria, would enhance plant growth under drought stress, and lay a foundation for developing a novel bioinoculant for mitigating abiotic stress and promoting sustainable little millet production.
- Published
- 2024
- Full Text
- View/download PDF
7. Apoplast-associated Bacillus amyloliquefaciens LAS10 for plant growth promotion and drought stress tolerance in little millet (Panicum sumatransae)
- Author
-
Monisha Rajkumar, Shobana Narayanasamy, and Sivakumar Uthandi
- Subjects
Endophytic bacteria ,Osmotic potential ,Bioinoculant ,Microbial interaction ,Plant growth ,Plant ecology ,QK900-989 - Abstract
Bacterial endophytes thriving in the apoplast could be tailored to withstand environmental challenges, thereby contributing to plant stress resilience. Developing plant growth-promoting (PGP) bacteria resilient to drought is crucial for sustainable agriculture and improved crop production under stress conditions. Thus, the present study aimed to decode the drought-tolerant apoplast-associated PGP bacteria from little millet (Panicum sumatrense L.) and profile its functional traits including the metabolites for PGP and drought resilience. The drought tolerant PGP bacterial isolates from apoplastic fluids of little millet (Var. ATL1) grown under stressed conditions (-10 bars) on polyethylene glycol (PEG) (PEG 6000) infused agar plates revealed that a total of 12 isolates, were initially screened for their ability to tolerate drought up to –36.6 bars (–3.6 MPa) and of which, 6 isolates (LAS1, LAS2, LAS4, LAS6, LAS9, and LAS10) were selected. Further, screening for their plant growth-promoting (PGP) traits such as 1-aminocyclopropane-1-carboxylate deaminase (ACCd) production, exopolysaccharide production, potassium (K), phosphorous (P), and Zinc (Zn) nutrition, indole acetic acid (IAA) production, siderophore production, ammonia, and hydrogen cyanide (HCN) exhibited higher PGP potentials in LAS10 followed by LAS2 and LAS4. Of three potential isolates (LAS10, LAS2, and LAS4), LAS10 produced the highest ACCd (147 n moles α- ketobutyrate mg–1h–1), IAA (15.9 μg mL–1), siderophore (54.9 % units) and P solubilization compared to other isolates. Further, isolate LAS10 was identified as Bacillus amyloliquefaciens based on 16S rRNA sequence analysis. Metabolite profiling of B. amyloliquefaciens LAS10 in GC–MS under induced stress conditions yielded several drought-tolerant metabolites belonging to the class viz., organic acids, fatty acids, amino acid and its derivatives, organoheterocyclic compounds, and benzenoids. The compounds responsible for drought tolerance such as phenol, proline, fumaric acid, ascorbic acid, and gibberellic acid are more pronounced under induced drought stress (PEG 6000) which would aid in drought stress tolerance and facilitate plant health and fitness. These results implied that B. amyloliquefaciens LAS10, an apoplast-associated drought-tolerant endophytic bacteria, would enhance plant growth under drought stress, and can be further extrapolated as a newer bio inoculant for abiotic stress mitigation and sustainable production in little millet.
- Published
- 2024
- Full Text
- View/download PDF
8. Flagellin, a plant-defense-activating protein identified from Xanthomonas axonopodis pv. Dieffenbachiae invokes defense response in tobacco
- Author
-
Tamilarasi Mani, J. Beslin Joshi, R. Priyadharshini, Jeya Sundara Sharmila, and Sivakumar Uthandi
- Subjects
Secretome ,Xanthomonas axonopodis ,Flagellin ,Plant-microbe interaction ,Defense-related enzymes ,Defense genes ,Microbiology ,QR1-502 - Abstract
Abstract Background Secretome analysis is a valuable tool to study host-pathogen protein interactions and to identify new proteins that are important for plant health. Microbial signatures elicit defense responses in plants, and by that, the plant immune system gets triggered prior to pathogen infection. Functional properties of secretory proteins from Xanthomonas axonopodis pv. dieffenbachiae (Xad1) involved in priming plant immunity was evaluated. Results In this study, the secretome of Xad1 was analyzed under host plant extract-induced conditions, and mass spectroscopic analysis of differentially expressed protein was identified as plant-defense-activating protein viz., flagellin C (FliC). The flagellin and Flg22 peptides both elicited hypersensitive reaction (HR) in non-host tobacco, activated reactive oxygen species (ROS) scavenging enzymes, and increased pathogenesis-related (PR) gene expression viz., NPR1, PR1, and down-regulation of PR2 (β-1,3-glucanase). Protein docking studies revealed the Flg22 epitope of Xad1, a 22 amino acid peptide region in FliC that recognizes plant receptor FLS2 to initiate downstream defense signaling. Conclusion The flagellin or the Flg22 peptide from Xad1 was efficient in eliciting an HR in tobacco via salicylic acid (SA)-mediated defense signaling that subsequently triggers systemic immune response epigenetically. The insights from this study can be used for the development of bio-based products (small PAMPs) for plant immunity and health.
- Published
- 2023
- Full Text
- View/download PDF
9. Activity of volatiles induced by microbes and natural plants stifled the growth of Pythium aphanidermatum - the damping off in Tomato
- Author
-
Praveen Thangaraj, Krishnamoorthy Akkanna Subbiah, Nakkeeran Sevugapperumal, Sivakumar Uthandi, Amirtham Damodarasamy, and Haripriya Shanmugam
- Subjects
M. spicata ,T. asperellum ,Volatilomes ,P. aphanidermatum ,Plant pathogen management ,Botany ,QK1-989 - Abstract
Abstract Background Volatilomes from natural plants and microbes imparts diverse antifungal properties to suppress the growth of plant pathogens and therefore can be a suitable alternative of chemical fungicides. The present experiment was to study effect of volatiles produced by natural plants and microbes on the fungal growth of Pythium aphanidermatum, which is a tomato seedling pathogen. Results Isolate of P. aphanidermatum, causing damping off in tomato were isolated and incubated at 25 ± 2 °C. The isolate was tested for the anti-oomycetes activities of volatiles in vitro. The volatiles produced by the leaves of Mentha spicata and Cymbopogon citratus showed the maximum inhibitory effect of 45.56 and 24.70 percent, respectively on the mycelial growth of P. aphanidermatum, whereas, the pathogen was not inhibited on exposure to the volatiles of macro-basidiomycetes fungi. The volatiles of T. asperellum showed the maximum inhibitory effect of 69.26 percent against P. aphanidermatum. The study also included the identification of Volatile Organic Compounds (VOCs) involved in the suppression of pathogens by Headspace Gas Chromatography Mass Spectrometry (HS GCMS). The results revealed the production of carvone by the leaves of M. spicata; citronellol and geraniol by C. citratus; isopentyl alcohol and limonene by T. asperellum with increased peak area percentage and these compounds possessed antifungal properties. The vaporous action of isopentyl alcohol completely suppressed the mycelial growth of P. aphanidermatum, which is highly correlated to the T. asperellum extract on pathogenic growth. While the compounds, carvone, and citronellol showed the maximum inhibitory effect of 89.02 and 85.49 percent, respectively when used at 500 ppm and also altered the sporulation behavior of P. aphanidermatum. Conclusion Results showed that volatiles of M. spicata and T. asperellum have anti-oomycetes action on pathogenic growth leading to a distortion of sporulation of P. aphanidermatum. High antifungal properties make VOCs suitable for incorporation as a new integrated plant disease management programs.
- Published
- 2023
- Full Text
- View/download PDF
10. Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs
- Author
-
Johnson Beslin Joshi, R. Priyadharshini, and Sivakumar Uthandi
- Subjects
Hemicellulose ,Hydrolysis ,Xylanase ,xynA ,Thermophiles ,Microbiology ,QR1-502 - Abstract
Abstract Background Hemicellulose is one of the copious polymer in lignocellulosic biomass (LCB). It is primarily composed of xylan linked by β-1,4 glycosidic bonds. Xylanase preferentially cleaves the β-1,4-glycosidic bonds in the xylan backbone resulting in complete hydrolysis of the biomass. Thermostable variants of glycoside hydrolases act as robust catalysts, not only in degradation but also during processing, to obtain specific carbohydrate-containing chemicals and materials (Ramasamy et al. in Madras Agric J 107(special):1. https://doi.org/10.29321/MAJ.2020.000382 , 2020). Results The xylanase production by two thermophilic bacteria isolated from thermal springs was evaluated. In addition, the gene encoding this industrially vital enzyme was isolated and characterized, and its protein structure was analyzed. The thermophilic bacteria producing xylanases were isolated from augmented sawdust and banana fiber biomass from hot springs of Himachal Pradesh and identified as Bacillus subtilis VSDB5 and Bacillus licheniformis KBFB4 using 16S rRNA gene sequencing. The persistent xylanase activity revealed that the enzyme is secreted extracellularly with the maximum activity of 0.76 IU mL−1 and 1.0 IU mL−1 at 6 h and 12 h of growth by KBFB4 and VSDB5, respectively, under submerged fermentation. Both the strains exhibited the maximum activity at pH 6 and a temperature of 50 °C. The xylanases of KBFB4 and VSDB5 were thermostable and retained 40% of their activity at 60 °C after incubation for 30 min. Xylanase of VSDB5 had wide thermotolerance and retained 20% of its activity from 60 to 80 °C, whereas xylanase of KBFB4 showed wide alkali tolerance and retained 80% of its activity until pH 10. The xylanase (xynA)-encoding gene (650 bp) cloned from both the strains using specific primers showed 98 to 99% homology to β-1,4-endoxylanase gene. Further in silico analysis predicted that the xylanase protein, with a molecular weight of 23 kDa, had a high pI (9.44–9.65), which explained the alkaline nature of the enzyme and greater aliphatic index (56.29). This finding suggested that the protein is thermostable. Multiple sequence alignment and homology modeling of the protein sequence revealed that the gene product belonged to the GH11 family, indicating its possible application in bioconversion. Conclusion The strains B. subtilis VSDB5 and B. licheniformis KBFB4 obtained from hot springs of Himachal Pradesh produced potent and alkali-tolerant thermostable xylanases, which belong to the GH11 family. The enzyme can be supplemented in industrial applications for biomass conversion at high temperatures and pH (or in processes involving alkali treatment). Graphical Abstract
- Published
- 2022
- Full Text
- View/download PDF
11. Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)
- Author
-
Naganandhini Srinivasan, Kiruthika Thangavelu, and Sivakumar Uthandi
- Subjects
Aspergillus ,Lovastatin ,Sago wastewater ,Bioassay ,Microbiology ,QR1-502 - Abstract
Abstract Background Lovastatin is one of the first statins to be extensively used for its cholesterol-lowering ability. It is commercially produced by fermentation. Species belonging to the genus Aspergillus are well-studied fungi that have been widely used for lovastatin production. In the present study, we produced lovastatin from sago processing wastewater (SWW) under submerged fermentation using oleaginous fungal strains, A. terreus KPR12 and A. caespitosus ASEF14. Results The intra- and extracellular concentrations of lovastatin produced by A. terreus KPR12 and A. caespitosus ASEF14 were lactonized. Because A. caespitosus ASEF14 produced a negligible amount of lovastatin, further kinetics of lovastatin production in SWW was studied using the KPR12 strain for 9 days. Lovastatin concentrations in the intra- and extracellular fractions of the A. terreus KPR12 cultured in a synthetic medium (SM) were 117.93 and 883.28 mg L–1, respectively. However, these concentrations in SWW were 142.23 and 429.98 mg L–1, respectively. The yeast growth inhibition bioassay confirmed the antifungal property of fungal extracts. A. terreus KPR12 showed a higher inhibition zone of 14 mm than the ASEF14 strain. The two-way analysis of variance (ANOVA; p
- Published
- 2022
- Full Text
- View/download PDF
12. Aspergillus caespitosus ASEF14, an oleaginous fungus as a potential candidate for biodiesel production using sago processing wastewater (SWW)
- Author
-
Naganandhini Srinivasan, Kiruthika Thangavelu, Ashika Sekar, B. Sanjeev, and Sivakumar Uthandi
- Subjects
Aspergillus ,Biodiesel ,Lipid content ,Sago wastewater ,Decontamination ,Microbiology ,QR1-502 - Abstract
Abstract Background Oleaginous microorganisms are sustainable alternatives for the production of biodiesel. Among them, oleaginous fungi are known for their rapid growth, short life cycles, no light requirement, easy scalability, and the ability to grow in cheap organic resources. Among all the sources used for biodiesel production, industrial wastewater streams have been least explored. We used oleaginous fungi to decontaminate sago processing wastewater and produce biodiesel. Results Among the 15 isolates screened for lipid production and starch utilization using the Nile red staining assay and amylase plate screening, three isolates accumulated > 20% (w/w) of their dry cell mass as lipids. The isolate ASEF14 exhibited the highest lipid accumulation (> 40%) and was identified as Aspergillus caespitosus based on the 28S rRNA gene sequencing. The maximum lipid content of 54.4% in synthetic medium (SM) and 37.2% in sago processing wastewater (SWW) was produced by the strain. The Fourier-transform infrared (FTIR) spectroscopy of the fungal oil revealed the presence of functional peaks corresponding to major lipids. Principal component analysis (PCA) of the FTIR data revealed major changes in the fatty acid composition during the transition from the growth phase (Days 1–3) to the lipid accumulation phase (Days 4–7). The fatty acid methyl esters (FAME) analysis of fungal oil from SWW contained 43.82% and 9.62% of saturated and monounsaturated fatty acids, respectively. The composition and percentage of individual FAME derived from SWW were different from SM, indicating the effect of nutrient and fermentation time. The fuel attributes of the SM- and SWW-grown fungal biodiesel (kinematic viscosity, iodine value, cetane number, cloud and pour point, linolenic acid content, FA > 4 double bonds) met international (ASTM D6751, EN 14214) and national (IS 15607) biodiesel standards. In addition to biodiesel production, the strain removed various contaminants such as total solids (TS), total suspended solids (TSS), total dissolved solids (TDS), dissolved oxygen (DO), chemical oxygen demand (COD), biological oxygen demand (BOD), total nitrogen (TN), total phosphorus (TP), and cyanide up to 58.6%, 53.0%, 35.2%, 94.5%, 89.3%, 91.3%, 74.0%, 47.0%, and 53.84%, respectively, from SWW. Conclusion These findings suggested that A. caespitosus ASEF14 is a potential candidate with high lipid accumulating ability (37.27%), capable of using SWW as the primary growth medium. The medium and incubation time alter the FAME profile of this fungus. The physical properties of fungal oil were in accordance with the biodiesel standards. Moreover, it decontaminated SWW by reducing several polluting nutrients and toxicants. The fungal biodiesel produced by this cost-effective method could serve as an alternate path to meet global energy demand.
- Published
- 2021
- Full Text
- View/download PDF
13. Bioconversion of sago processing wastewater into biodiesel: Optimization of lipid production by an oleaginous yeast, Candida tropicalis ASY2 and its transesterification process using response surface methodology
- Author
-
Kiruthika Thangavelu, Pugalendhi Sundararaju, Naganandhini Srinivasan, and Sivakumar Uthandi
- Subjects
Sago processing wastewater ,Yeast lipid ,Transesterification ,Biodiesel ,Response surface methodology ,Microbiology ,QR1-502 - Abstract
Abstract Background Biodiesel is an eco-friendly and renewable energy source and a valuable substitute for petro-diesel. Sago processing wastewater (SWW), a by-product of the cassava processing industry, has starch content ranging from 4 to 7 g L–1 and serves as an outstanding source for producing microbial lipids by the oleaginous microorganisms. In the present study, Candida tropicalis ASY2 was employed to optimize single-cell oil (SCO) production using SWW and subsequent transesterification by response surface methodology. Variables such as starch content, yeast extract, airflow rate, pH, and temperature significantly influenced lipid production in a preliminary study. The lipid production was scaled up to 5 L capacity airlift bioreactor and its optimization was done by response surface methodology. The dried yeast biomass obtained under optimized conditions from 5 L bioreactor was subjected to a direct transesterification process. Biomass: methanol ratio, catalyst concentration, and time were the variables used to attain higher FAME yield in the transesterification optimization process. Results Under optimized conditions, the highest lipid yield of 2.68 g L–1 was obtained with 15.33 g L–1 of starch content, 0.5 g L–1 of yeast extract, and 5.992 L min–1 of airflow rate in a bioreactor. The optimized direct transesterification process yielded a higher FAME yield of 86.56% at 1:20 biomass: methanol ratio, 0.4 M catalyst concentration, and a time of 6.85 h. Conclusions Thus, this optimized process rendered the microbial lipids derived from C. tropicalis ASY2 as potentially alternative oil substitutes for sustainable biodiesel production to meet the rising energy demands.
- Published
- 2021
- Full Text
- View/download PDF
14. Tyrosinase and laccase-producing Bacillus aryabhattai TFG5 and its role in the polymerization of phenols
- Author
-
Iniyakumar Muniraj, Syed Shameer, and Sivakumar Uthandi
- Subjects
Bacillus aryabhattai TFG5 ,Tyrosinase ,Laccase ,ARDRA ,Polymerization of phenols ,FT-IR ,Microbiology ,QR1-502 - Abstract
Abstract Background Tyrosinases and laccases are oxidoreductase enzymes that are used widely in the food, feed, textile, and biofuel industries. The rapidly growing industrial demand for bacterial oxido-reductases has encouraged research on this enzyme worldwide. These enzymes also play a key role in the formation of humic substances (HS) that are involved in controlling the biogeochemical carbon cycle, providing nutrients and bio-stimulants for plant growth, and interacting with inorganic and organic pollutants besides increasing carbon sequestration and mitigating greenhouse gas emission in the environment. The present study aimed to screen and characterize extracellular tyrosinase and laccase-producing soil bacteria that could be utilized in the polymerization of phenols. Results Twenty isolates from different soil samples collected from forest ecosystems were characterized through ARDRA using restriction digestion with AluI, HpaII, and HaeIII restriction enzymes. The results of Hierarchical Cluster Analysis (HCA) revealed a 60 % similarity coefficient among 13 out of 20 isolates, of which, the isolate TFG5 exhibited only 10 % similarity when compared to all the other isolates. The isolate TFG5 exhibited both tyrosinase (1.34 U.mL− 1) and laccase (2.01 U.mL− 1) activity and was identified as Bacillus aryabhattai. The increased polymerization activity was observed when B. aryabhattai TFG5 was treated with phenols. The monomers such as catechol, p-Hydroxy benzoic acid, ferulic acid, and salicylic acid were polymerized efficiently, as evidenced by their FT-IR spectra depicting increased functional groups compared to the standard mushroom tyrosinase. Conclusions The polymerization ability of B. aryabhattai TFG5 could be applied to phenol-rich wastewater treatment for efficient precipitation of phenols. Furthermore, tyrosinases can be used for enhancing the synthesis of HS in soil.
- Published
- 2021
- Full Text
- View/download PDF
15. Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes
- Author
-
M. Balakrishnan, G. Jeevarathinam, S. Kiran Santhosh Kumar, Iniyakumar Muniraj, and Sivakumar Uthandi
- Subjects
α-Amylase ,Solid-state fermentation ,Aspergillus oryzae ,Edible oil cake ,Pilot-scale fermenter ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background Amylases produced by fungi during solid-state fermentation are the most widely used commercial enzymes to meet the ever-increasing demands of the global enzyme market. The use of low-cost substrates to curtail the production cost and reuse solid wastes are seen as viable options for the commercial production of many enzymes. Applications of α-amylases in food, feed, and industrial sectors have increased over the years. Additionally, the demand for processed and ready-to-eat food has increased because of the rapid growth of food-processing industries in developing economies. These factors significantly contribute to the global enzyme market. It is estimated that by the end of 2024, the global α-amylase market would reach USD 320.1 million (Grand View Research Inc., 2016). We produced α-amylase using Aspergillus oryzae and low-cost substrates obtained from edible oil cake, such as groundnut oil cake (GOC), coconut oil cake (COC), sesame oil cake (SOC) by solid-state fermentation. We cultivated the fungus using these nutrient-rich substrates to produce the enzyme. The enzyme was extracted, partially purified, and tested for pH and temperature stability. The effect of pH, incubation period and temperature on α-amylase production using A. oryzae was optimized. Box–Behnken design (BBD) of response surface methodology (RSM) was used to optimize and determine the effects of all process parameters on α-amylase production. The overall cost economics of α-amylase production using a pilot-scale fermenter was also studied. Results The substrate optimization for α-amylase production by the Box–Behnken design of RSM showed GOC as the most suitable substrate for A. oryzae, as evident from its maximum α-amylase production of 9868.12 U/gds. Further optimization of process parameters showed that the initial moisture content of 64%, pH of 4.5, incubation period of 108 h, and temperature of 32.5 °C are optimum conditions for α-amylase production. The production increased by 11.4% (10,994.74 U/gds) by up-scaling and using optimized conditions in a pilot-scale fermenter. The partially purified α-amylase exhibited maximum stability at a pH of 6.0 and a temperature of 55 °C. The overall cost economic studies showed that the partially purified α-amylase could be produced at the rate of Rs. 622/L. Conclusions The process parameters for enhanced α-amylase secretion were analyzed using 3D contour plots by RSM, which showed that contour lines were more oriented toward incubation temperature and pH, having a significant effect (p
- Published
- 2021
- Full Text
- View/download PDF
16. Metabolites of Trichoderma longibrachiatum EF5 inhibits soil borne pathogen, Macrophomina phaseolina by triggering amino sugar metabolism
- Author
-
Sridharan, A.P., Sugitha, Thangappan, Karthikeyan, G., Nakkeeran, S., and Sivakumar, Uthandi
- Published
- 2021
- Full Text
- View/download PDF
17. Bacillus aryabhattai TFG5-mediated synthesis of humic substances from coir pith wastes
- Author
-
Iniyakumar Muniraj, Syed Shameer, Priyadharshini Ramachandran, and Sivakumar Uthandi
- Subjects
Bacillus aryabhattai TFG5 ,Tyrosinase ,Laccase ,Coir pith biomass ,Oxidative polymerization ,Humic substances synthesis ,Microbiology ,QR1-502 - Abstract
Abstract Background Humic substances (HS) form the largest proportion among all the constituents of soil organic matter and are a key component of the terrestrial ecosystem. HS plays a multifunctional role in the environment by controlling the biogeochemical carbon cycle, providing nutrients and bio-stimulants for plant growth, and interacting with inorganic and organic pollutants. The rate of formation of HS in soils determines its productivity and carbon sequestration capacity. Enhancement of HS synthesis in the soil through the microbial route not only increases CO2 sequestration but also mitigates the greenhouse gas emissions in the environment. Result In this study, we attempted to understand the mechanism of formation and enhancement of HS from coir pith wastes using the tyrosinase produced by Bacillus aryabhattai TFG5. The bacterium TFG5 isolated from the termite garden produced the tyrosinase (1.34 U mL−1) and laccase (2.1 U mL−1) at 48 h and 60 h of fermentation, respectively. The extracellular tyrosinase from B. aryabhattai TFG5 was designated as TyrB. Homology modeling of TyrB revealed a structure with a predicted molecular mass of 35.23 kDa and two copper ions in the active center with its conserved residues required for the tyrosinase activity. TyrB efficiently transformed and polymerized standard phenols, such as p-cresol, p-hydroxyl benzoic acid, Levo DOPA, and 2,6 DMP, besides transforming free phenols in coir pith wash water (CWW). Additionally, UV–Vis and FT-IR spectra of the degradation products of the coir pith treated with TyrB revealed the formation of HS within 3 days of incubation. Furthermore, the E472/664 ratio of the degradation products revealed a higher degree of condensation of the aromatic carbons and the presence of more aliphatic structures in the HS. Conclusion The results confirmed the influence of TyrB for the effective synthesis of HS from coir pith wastes. The results of the present study also confirm the recently accepted theory of humification proposed by the International Humic Substances Society.
- Published
- 2021
- Full Text
- View/download PDF
18. Unmanned Aerial Vehicle-Measured Multispectral Vegetation Indices for Predicting LAI, SPAD Chlorophyll, and Yield of Maize.
- Author
-
Parida, Pradosh Kumar, Somasundaram, Eagan, Krishnan, Ramanujam, Radhamani, Sengodan, Sivakumar, Uthandi, Parameswari, Ettiyagounder, Raja, Rajagounder, Shri Rangasami, Silambiah Ramasamy, Sangeetha, Sundapalayam Palanisamy, and Gangai Selvi, Ramalingam
- Subjects
LEAF area index ,STANDARD deviations ,PLANT phenology ,MULTISPECTRAL imaging ,AGRICULTURE - Abstract
Predicting crop yield at preharvest is pivotal for agricultural policy and strategic decision making. Despite global agricultural targets, labour-intensive surveys for yield estimation pose challenges. Using unmanned aerial vehicle (UAV)-based multispectral sensors, this study assessed crop phenology and biotic stress conditions using various spectral vegetation indices. The goal was to enhance the accuracy of predicting key agricultural parameters, such as leaf area index (LAI), soil and plant analyser development (SPAD) chlorophyll, and grain yield of maize. The study's findings demonstrate that during the kharif season, the wide dynamic range vegetation index (WDRVI) showcased superior correlation coefficients (R), coefficients of determination (R
2 ), and the lowest root mean square errors (RMSEs) of 0.92, 0.86, and 0.14, respectively. However, during the rabi season, the atmospherically resistant vegetation index (ARVI) achieved the highest R and R2 and the lowest RMSEs of 0.83, 0.79, and 0.15, respectively, indicating better accuracy in predicting LAI. Conversely, the normalised difference red-edge index (NDRE) during the kharif season and the modified chlorophyll absorption ratio index (MCARI) during the rabi season were identified as the predictors with the highest accuracy for SPAD chlorophyll prediction. Specifically, R values of 0.91 and 0.94, R2 values of 0.83 and 0.82, and RMSE values of 2.07 and 3.10 were obtained, respectively. The most effective indices for LAI prediction during the kharif season (WDRVI and NDRE) and for SPAD chlorophyll prediction during the rabi season (ARVI and MCARI) were further utilised to construct a yield model using stepwise regression analysis. Integrating the predicted LAI and SPAD chlorophyll values into the model resulted in higher accuracy compared to individual predictions. More exactly, the R2 values were 0.51 and 0.74, while the RMSE values were 9.25 and 6.72, during the kharif and rabi seasons, respectively. These findings underscore the utility of UAV-based multispectral imaging in predicting crop yields, thereby aiding in sustainable crop management practices and benefiting farmers and policymakers alike. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
19. Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1
- Author
-
K. Aswini, N. O. Gopal, and Sivakumar Uthandi
- Subjects
Bacterial cellulose ,Acetobacter senegalensis ,Optimization ,RSM ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants. However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin. On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum. A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose. Besides, the production is also influenced by other culture conditions. Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced. Results The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time. Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM). Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation. The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L− 1 of glycerol, 7.50 g L− 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L− 1 of PEG 6000. This gave a BC yield of wet weight as 469.83 g L− 1. Conclusion The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium. Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.
- Published
- 2020
- Full Text
- View/download PDF
20. Thermotolerant glycosyl hydrolases-producing Bacillus aerius CMCPS1 and its saccharification efficiency on HCR-laccase (LccH)-pretreated corncob biomass
- Author
-
Meena Ganesan, Remitha Mathivani Vinayakamoorthy, Sugitha Thankappan, Iniyakumar Muniraj, and Sivakumar Uthandi
- Subjects
Glycoside hydrolases ,B. aerius ,Hot springs ,β-Glucosidase ,Cellulases ,HCR-LccH ,Fuel ,TP315-360 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background The current production of bioethanol based on lignocellulosic biomass (LCB) highly depends on thermostable enzymes and extremophiles owing to less risk of contamination. Thermophilic bacterial cellulases are preferred over fungi due to their higher growth rate, presence of complex multi-enzymes, stability, and enhanced bioconversion efficiency. Corncob, underutilized biomass, ensures energy conservation due to high lignocellulosic and more fermentable sugar content. In the present study, the thermophilic bacterium Bacillus aerius CMCPS1, isolated from the thermal springs of Manikaran, Himachal Pradesh, India, was characterized in terms of its activity, stability, and hydrolytic capacity. A two-step process comprising: (i) a combined strategy of hydrodynamic cavitation reaction (HCR)-coupled enzymatic (LccH at 6.5 U) pretreatment for delignification and (ii) subsequent hydrolysis of pre-treated (HCR-LccH) corncob biomass (CCB) using a thermostable cocktail of CMCPS1 was adopted to validate the efficiency of the process. Some of the parameters studied include lignin reduction, cellulose increase, and saccharification efficiency. Result Among the five isolates obtained by in situ enrichment on various substrates, B. aerius CMCPS1, isolated from hot springs, exhibited the maximum hydrolytic activity of 4.11. The GH activity of the CMCPS1 strain under submerged fermentation revealed maximum filter paper activity (FPA) and endoglucanase activity of 4.36 IU mL−1 and 2.98 IU mL−1, respectively, at 44 h. Similarly, the isolate produced exoglucanase and β-glucosidase with an activity of 1.76 IU mL−1 and 1.23 IU mL−1 at 48 h, respectively. More specifically, the enzyme endo-1,4-β-d glucanase E.C.3.2.1.4 (CMCase) produced by B. aerius CMCPS1 displayed wider stability to pH (3–9) and temperature (30–90 °C) than most fungal cellulases. Similarly, the activity of CMCase increased in the presence of organic solvents (118% at 30% acetone v/v). The partially purified CMCase from the culture supernatant of CMCPS1 registered 64% yield with twofold purification. The zymogram and SDS-PAGE analyses further confirmed the CMCase activity with an apparent molecular mass of 70 kDa. The presence of genes specific to cellulases, such as cellulose-binding domain CelB, confirmed the presence of GH family 46 and β-glucosidase activity (GH3). The multifunctional cellulases of CMCPS1 were evaluated for their saccharification efficiency on laccase (LccH, a fungal laccase from Hexagonia hirta MSF2)-pretreated corncob in a HCR. The lignin and hemicelluloses removal efficiency of HCR-LccH was 54.1 and 6.57%, respectively, with an increase in cellulose fraction (42.25%). The saccharification efficiency of 55% was achieved with CMCPS1 multifunctional cellulases at 50 °C and pH 5.0. Conclusion The multifunctional cellulase complex of B. aerius CMCPS1 is a potential biocatalyst for application in lignocellulosic biomass-based biorefineries. The saccharification ability of HCR-LccH-pretreated corncob at elevated temperatures would be an advantage for biofuel production from lignocellulosic biomass.
- Published
- 2020
- Full Text
- View/download PDF
21. Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2
- Author
-
Kiruthika Thangavelu, Pugalendhi Sundararaju, Naganandhini Srinivasan, Iniyakumar Muniraj, and Sivakumar Uthandi
- Subjects
Sago wastewater ,Starch ,Oleaginous yeast ,Lipid production ,Biodiesel ,Fuel ,TP315-360 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable alternative based on renewable and accessible feedstock. One of the promising solutions for the current and near-future is the substitution of fossil fuels with sustainable liquid feedstock for biofuel production. Among the different renewable liquid feedstock’s studied, wastewater is the least explored one for biodiesel production. Sago wastewater is the byproduct of the cassava processing industry and has starch content ranging from 4 to 7%. The present investigation was aimed to produce microbial lipids from oleaginous yeast, Candida tropicalis ASY2 for use as biodiesel feedstock and simultaneously decontaminate the sago processing wastewater for reuse. Initial screening of oleaginous yeast to find an efficient amylolytic with maximum lipid productivity resulted in a potent oleaginous yeast strain, C. tropicalis ASY2, that utilizes SWW as a substrate. Shake flask experiments are conducted over a fermentation time of 240 h to determine a suitable fatty acid composition using GC-FID for biodiesel production with simultaneous removal of SWW pollutants using ASY2. Results The maximum biomass of 0.021 g L−1 h−1 and lipid productivity of 0.010 g L−1 h−1 was recorded in SWW with lipid content of 49%. The yeast strain degraded cyanide in SWW (79%) and also removed chemical oxygen demand (COD), biological oxygen demand (BOD), nitrate (NO3), ammoniacal (NH4), and phosphate (PO4) ions (84%, 92%, 100%, 98%, and 85%, respectively). GC-FID analysis of fatty acid methyl esters (FAME) revealed high oleic acid content (41.33%), which is one of the primary fatty acids for biodiesel production. Conclusions It is evident that the present study provides an innovative and ecologically sustainable technology that generates valuable fuel, biodiesel using SWW as a substrate and decontaminates for reuse.
- Published
- 2020
- Full Text
- View/download PDF
22. Antifungal Volatilomes Mediated Defense Mechanism against Fusarium oxysporum f. sp. lycopersici, the Incitant of Tomato Wilt
- Author
-
Praveen Thangaraj, Akshaya Subbaih Balamurali, Krishnamoorthy Akkanna Subbiah, Nakkeeran Sevugapperumal, Thiribhuvanamala Gurudevan, Sivakumar Uthandi, and Haripriya Shanmugam
- Subjects
volatilomes ,phyto-fumigants ,antifungal action ,Fusarium oxysporum ,Organic chemistry ,QD241-441 - Abstract
In this study, the volatilomes of naturally growing plant leaves were immobilized in a suitable substrate to enhance vapors’ diffusion in the soil to eradicate the Fusarium wilt pathogens in Tomato. Volatilomes produced by Mentha spicata leaves immobilized in vermiculite ball was found to be effective and exhibit 92.35 percent inhibition on the mycelial growth of Fusarium oxysporum f. sp. lycopersici (FOL). Moreover, the volatilomes of M. spicata immobilized vermiculite balls were tested based on the distance traveled by the diffused volatilomes from the ball and revealed that the volatilomes of M. spicata traveled up to 20 cm distance from the center of PVC (Polyvinly chloride) chamber showed maximum reduction in colony growth of FOL at 12th day after inoculation. Tomato plants inoculated with FOL revealed increased expressions of defense gene, pathogenesis related protein (PR1) with 2.63-fold after 72 h and the gene, transcription factor (WRKY) increased with 2.5-fold after 48 h on exposure to the volatilomes of M. spicata vermiculite balls. To the best of our knowledge, this is the first report on development of volatilomes based vermiculite ball formulations. This result indicated that the volatilomes of M. spicata are promising phyto-fumigants for management of Tomato Fusarial wilt.
- Published
- 2022
- Full Text
- View/download PDF
23. Targeting bone cancer with 4-Allylbenzene-1,2-diol purified from Piper betle L.: an in silico and cytotoxicity scrutiny
- Author
-
Vinusri Sekar, Gnanam Ramasamy, Caroline Ravikumar, Raveendran Muthurajan, Sivakumar Uthandi, Rajamani Kandasamy, and Patil Santosh Ganapati
- Subjects
Structural Biology ,General Medicine ,Molecular Biology - Published
- 2023
24. Bioprospecting thermophilic glycosyl hydrolases, from hot springs of Himachal Pradesh, for biomass valorization
- Author
-
Sugitha Thankappan, Sujatha Kandasamy, Beslin Joshi, Ksenia N. Sorokina, Oxana P. Taran, and Sivakumar Uthandi
- Subjects
Glycosyl hydrolases ,Thermophilic ,Biomass ,Hot springs ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Abstract The harnessing of biocatalysts from extreme environment hot spring niche for biomass conversion is significant and promising owing to the special characteristics of extremozymes attributed by intriguing biogeochemistry and extreme conditions of these environments. Hence, in the present study 38 bacterial isolates obtained from hot springs of Manikaran (~ 95 °C), Kalath (~ 50 °C) and Vasist (~ 65 °C) of Himachal Pradesh were screened for glycosyl hydrolases by in situ enrichment technique using lignocellulosic biomass (LCB). Based on their hydrolytic potential 5 isolates were selected and they were Bacillus tequilensis (VCB1, VCB2 and VSDB4), and B. licheniformis (KBFB2 and KBFB3). Cellulolytic activity assayed by growth under submerged fermentation showed that B. tequilensis VCB1 had maximum FPA activity (3.38 IU ml−1) in 48 h, while B. licheniformis KBFB3 excelled for endoglucanase (EGA of 4.81 IU ml−1 in 24 h) and cellobiase (0.71 IU ml−1 in 48 h) activities. Among all the thermophilic biocatalysts evaluated, highest exoglucanase (0.06 IU ml−1) activity was observed in B. tequilensis VSDB4 while endoglucanase of B. licheniformis KBFB3 showed optimum specific activity at pH 7 and 70 °C. Further, the presence of celS, celB and xlnB genes in the isolates suggest their possible role in biomass conversion. Protein profiling by SDS-PAGE analysis revealed that cellulase isoforms migrated with molecular masses of 75 kDa. The endoglucanase activity of promising strain B. licheniformis KBFB3 was enhanced in the presence of Ca2+, mercaptoethanol and sodium hypochlorite whereas moderately inhibited by Cu2+, Zn2+, urea, SDS and H2O2. The results of this study indicate scope for the possible development of novel biocatalysts with multifunctional thermostable glycosyl hydrolases from hot springs for efficient hydrolysis of the complex lignocellulosic biomass into simple sugars and other derived bioproducts leading to biomass valorization.
- Published
- 2018
- Full Text
- View/download PDF
25. Delignification of corncob via combined hydrodynamic cavitation and enzymatic pretreatment: process optimization by response surface methodology
- Author
-
Kiruthika Thangavelu, Ramesh Desikan, Oxana P. Taran, and Sivakumar Uthandi
- Subjects
Corncob ,Pretreatment ,Delignification ,Hydrodynamic cavitation ,Laccase enzyme ,Fuel ,TP315-360 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background Renewable liquid biofuel production will reduce crude oil import of India. To displace the huge quantity of fossil fuels used for energy production, this research was focused on utilization of unexploited low-cost agricultural residues for biofuel production. Corncobs are a byproduct of corn processing industry, and till now it is not utilized for biofuel production, eventhough it has high lignocellulosic concent. In this study, combined hydrodynamic cavitation and enzymatic (HCE) method was evaluated as a pretreatment method of corncob for biofuel production. The most significant process parameters namely (i) enzyme loading (3–10 U g−1), (ii) biomass loading (2.5–5.0%), and (iii) duration (5–60 min) were optimized and their effects on combined HCE pretreatment of corncob was studied through response surface methodology for lignin reduction, hemicellulose reduction and cellulose increase. Results The highest lignin reduction (47.4%) was obtained in orifice plate 1 (OP1) under the optimized conditions namely biomass loading at 5%, enzyme loading at 6.5 U g−1 of biomass, and reaction duration of 60 min. The above tested independent variables had a significant effect on lignin reduction. The cavitational yield and energy consumption under the above-mentioned optimized conditions for OP1 was 3.56 × 10−5 g J−1 and 1.35 MJ kg−1, respectively. Conclusions It is evident from the study that HCE is an effective technology and requires less energy (1.35 MJ kg−1) than other biomass pretreatment methods.
- Published
- 2018
- Full Text
- View/download PDF
26. Insights into the Metabolites Conferring Pathogenicity of Xanthomonas oryzae and Its Inhibition by Trichoderma longibrachiatum EF5
- Author
-
Sivakumar Uthandi, Karthikeyan Gandhi, Sugitha Thankappan, and A. P. Sridharan
- Subjects
Psychiatry and Mental health - Abstract
Aims: The present study was aimed to evaluate the effect of volatile and soluble metabolites of Trichoderma longibrachiatum EF5 against Xanthomonas oryzae pv. oryzae, and to identify the metabolites produced by Xanthomonas oryzae pv. oryzae in culture filtrate. Study Design: In vitro bioassay with CRD. Place and Duration of Study: Biocatalysts laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 2019-2020. Methodology: Inverted plate, bipartite plate and agar well method was done under in vitro to observe the efficacy of T. longibrachiatum EF5 VOC against X. oryzae pv. oryzae. Analysis of Xanthomonas oryzae pv. oryzae metabolites by using Gas Chromatography and Mass Spectrometry (GC-MS). Results: T. longibrachiatum EF5 VOC and metabolites completely inhibited the growth of X. oryzae pv. oryzae in inverted plate assay, whereas in bipartite and agar well diffusion assays unmeasurable growth of X. oryzae pv. oryzae was observed. The metabolites or Diffusible signal factors such as butyrolactone, propionic acid derivatives, phenyl acetic acid, hydrofurans, picoxystrobin, benzoic acid derivatives were produced by X. oryzae pv. oryzae in the growing medium. The role of these metabolites revealed that they are involved in pathogenicity, virulence, quorum sensing and the synthesis of antioxidant. Conclusion: T. longibrachiatum EF5 volatile and soluble metabolites can be used as biocontrol agent against X. oryzae pv. oryzae.
- Published
- 2022
27. Bioactive Metabolites of Nodule Associated Microbes for Enhanced Drought Tolerance and Biocontrol Control Activity in Horsegram
- Author
-
Sivakumar Uthandi and Shirley Evangilene
- Subjects
General Medicine - Abstract
In the present study, the potential bioactive compounds detected in the ethyl acetate microbial extract of root nodules of horsegram were determined using the gas chromatography-mass spectroscopy (GC-MS). The bioactive metabolites of nodule associated microbes (NAM) revealed the existence of several soluble metabolites which includes phthalic acid, butyl hex-3-yl ester, 2,4-Di-tert-butylphenol,Dodecyl acrylate, pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro, 1-Nonadecene, octadecane, 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester, hexadecanoic acid, methyl ester, dibutyl phthalate, diisooctyl phthalate, 1-docosene, heptadecane, 9-hexyl, pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl), n-Tetracosanol-1, ergotaman-3',6',18-trione, 9,10-dihydro-12'-hydroxy-2'-methyl-5'(phenylmethyl)-, (5'à,10à), eicosanal, ethanol, 2-(9-octadecenyloxy), E-15-heptadecenal, octatriacontyl pentafluoropropionate, etc.,. Endophytic bacteria produces bioactive substances that provides resistance against phytopathogens as well as nutrient solubilization during prolonged drought periods in order to overcome biotic and abiotic stresses. Several antibacterial, antifungal, antiviral and antioxidant properties were brought under spotlight to realise the beneficial aspects of nodule associated microbes of horsegram. Understanding the roles of metabolites would enrich the crop growth under stressed environment by promoting the eco-friendly agriculture practices.
- Published
- 2022
28. Integration of Heavy Metal Pollution Indices and Health Risk Assessment of Groundwater in Semi-arid Coastal Aquifers, South Africa
- Author
-
Philisiwe P. Mthembu, Vetrimurugan Elumalai, Peiyue Li, Sivakumar Uthandi, N. Rajmohan, and S. Chidambaram
- Subjects
Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Pollution ,Water Science and Technology - Published
- 2022
29. Enhancement of disease resistance, growth potential, and photosynthesis in tomato (Solanum lycopersicum) by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus strain BPSAC147.
- Author
-
Ajit Kumar Passari, Kalidas Upadhyaya, Garima Singh, Ahmed M Abdel-Azeem, Sugitha Thankappan, Sivakumar Uthandi, Abeer Hashem, Elsayed Fathi Abd Allah, Jahangir Ahmed Malik, Alqarawi As, Vijai Kumar Gupta, Sanjay Ranjan, and Bhim Pratap Singh
- Subjects
Medicine ,Science - Abstract
Biotic stresses in plants have a significant impact on agricultural productivity. In the present study, in vivo experiments were conducted to determine the physiological responses of tomato (Solanum lycopersicum L.) seedlings by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus isolate BPSAC147 under greenhouse conditions. Further, photochemical quantum yield of photosystem II (PSII) (Fv/Fm), photochemical quenching (qP) and non-photochemical (NPQ) were calculated in seedlings inoculated with S. thermocarboxydus (T1) and were compared with control (T0) plants. Furthermore, the electron transport rate (ETR) of PSII exhibited a significant increase in T1 plants, relative to T0 plants. These results indicate that inoculation of tomato seedlings with S. thermocarboxydus had a positive effect on the process of photosynthesis, resulting in enhanced chlorophyll fluorescence parameters due to increased ETR in the thylakoid membrane. GC-MS analysis showed significant differences in the volatile compounds in the different treatments performed under greenhouse conditions. The present study suggests that S. thermocarboxydus can be used as new biocontrol agent to control Fusarium wilt in tomato crops and enhance productivity by enhancing photosynthesis.
- Published
- 2019
- Full Text
- View/download PDF
30. Release Kinetics of Iron (Fe) from Soil and Growing Media Mixtures: An Incubation Study
- Author
-
Prakash Ranjan Behera, Chitdeshwari Thiyagarajan, Malarvizhi P, Sivakumar Uthandi, and Irene Vethamoni P
- Subjects
Soil Science ,Agronomy and Crop Science - Published
- 2022
31. Glycosyl hydrolases of Bacillus spp. from the vermicasts of epigeic Eudrilus eugeniae for biomass valorization
- Author
-
Daphy Meurial Chandra, Sugitha Thankappan, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2023
32. New methods for the one-pot processing of polysaccharide components (cellulose and hemicelluloses) of lignocellulose biomass into valuable products. Part 2: Biotechnological approaches to the conversion of polysaccharides and monosaccharides into the valuable industrial chemicals
- Author
-
Sorokina, K. N., Samoylova, Yu. V., Piligaev, A. V., Sivakumar, Uthandi, and Parmon, V. N.
- Published
- 2017
- Full Text
- View/download PDF
33. New methods for the one-pot processing of polysaccharide components (cellulose and hemicelluloses) of lignocellulose biomass into valuable products. Part 3: Products synthesized via the biotechnological conversion of poly- and monosaccharides of biomass
- Author
-
Sorokina, K. N., Samoylova, Yu. V., Piligaev, A. V., Sivakumar, Uthandi, and Parmon, V. N.
- Published
- 2017
- Full Text
- View/download PDF
34. Isolation, purification, and identification of novel lignin-degrading Aspergillus caespitosus strain S2
- Author
-
Lakshmi Kasirajan, Keerthana Kamaraj, Julie A. Maupin-Furlow, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2022
35. Insights into the properties of bacterial cellulose produced by Acetobacter senegalensis MA1 and uncovering the novel bioactive metabolites of its spent medium
- Author
-
K. Aswini, N. O. Gopal, N. Shobana, Chitra Sudalaimani, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2022
36. Ascertaining gamma ray dosage sensitivity of in vitro cultures in banana cv. Ney Poovan (Musa AB)
- Author
-
Sivakumar Uthandi
- Subjects
fungi ,Soil Science ,food and beverages ,Plant culture ,Plant Science ,gamma irradiation-shoot tip cultures-lethal dosage-banana ,Agronomy and Crop Science ,SB1-1110 - Abstract
In a vegetatively propagated and parthenocarpic crop like banana, the mutation breeding method offers considerable scope to create genetic variability for crop improvement. It is necessary to determine the LD50 dose for optimizing the radiation dosages when gamma rays are used for mutation induction. In the present study, in order to fix the LD50 dose for explants cultured in vitro in cv. Ney Poovan, in vitro shoot tips were subjected to five different doses of gamma rays ranged from 5 to 25 Gy. Proliferating multiple shoots were subjected to five different doses of gamma rays ranged from 2 to 10 Gy. The results revealed a linear and significant reduction in the survival percentage, shoot length and number of shoots with increasing levels of gamma irradiation doses in both the category of explants. The probit curve based analysis on mortality of treated explants revealed that LD50 dose of gamma rays to be 18.77 Gy for cultured shoot tips and 6.97 Gy for proliferating multiple shoots under in vitro.
- Published
- 2021
37. Lignin derived aromatic monomers from birch wood by laccase (LccH) pretreatment and Ru/C catalyst: a two-pot approach for sustainable biorefineries
- Author
-
Sekar Nishanth, Andrey S Chikunov, Sugitha Thankappan, Oxana P Taran, Valentin N Parmon, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2022
38. Microbial Fermentation of Waste Oils for Production of Added-Value Products
- Author
-
Naganandhini Srinivasan, Kiruthika Thangavelu, and Sivakumar Uthandi
- Published
- 2022
39. A 16S rRNA amplicon approach to the structural and functional diversity of bacterial communities associated with horse gram crop for drought mitigation and sustainable productivity
- Author
-
Shirley Evangilene and Sivakumar Uthandi
- Subjects
Soil ,Bacteria ,Nitrogen ,RNA, Ribosomal, 16S ,Rhizosphere ,Fabaceae ,General Medicine ,Applied Microbiology and Biotechnology ,Soil Microbiology ,Biotechnology ,Droughts - Abstract
Aim In this study, 16S rRNA amplicon sequencing analyses were performed to determine the diversity of the bacterial community present in the soil, rhizosphere region, root nodules and seeds of the horse gram plant. Methods and Results We observed the dominance of Proteobacteria, Actinobacteria, Firmicutes, Acidobacteria, Bacteroidetes, Planctomycetes and Gemmatimonadetes across all four domains of the horse gram plant. For community analyses, the significance of the alpha diversity was estimated using the Shannon index, Simpson index and Chao1 index, which revealed no significant difference among the samples. However, the estimation of the beta diversity indicated a significant difference among the samples, with p < 0.001 and R2 = 1. A strong positive correlation was found between the rhizosphere and root nodule samples. Comparative genomics of the 16S rRNA gene showed that ammonium-oxidizing metabolism (amoA), nitrite-reducing metabolism (nirK) and nitrogen-fixing metabolism (nifH) were prominent mechanisms in all samples. The genes involved in the biosynthesis of amino acids, purine metabolism and nitrogen metabolism were identified as the key genes associated with the functional traits of microbial domains in horse gram. Conclusion The culturable microbes associated with horse gram can be used as a substitute for synthetic fertilizers to maintain soil fertility and ecological health in agricultural practices. Significance and Impact of the study Determining the survival strategies of bacterial communities that positively respond to multiple gate selection helps in understanding the structural diversity and functional traits primarily focused on the development of beneficial microbial consortium for promoting plant growth.
- Published
- 2022
40. Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (Spodoptera frugiperda) in Maize
- Author
-
Ranjith, Sellappan, primary, Kalaiselvi, Thangavel, additional, Muthusami, Muruganagounder, additional, and Sivakumar, Uthandi, additional
- Published
- 2022
- Full Text
- View/download PDF
41. Bioprotective potential of maize apoplastic fluid bacterium (Bacillus amyloliquefaciens) and arbuscular mycorrhizal fungi (Glomus intraradices) against Spodoptera frugiperda infestation in maize
- Author
-
Ranjith Sellappan, Kalaiselvi Thangavel, and Sivakumar Uthandi
- Subjects
Genetics ,Plant Science - Published
- 2023
42. Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis.
- Author
-
Vineet Kumar Mishra, Ajit Kumar Passari, Preeti Chandra, Vincent Vineeth Leo, Brijesh Kumar, Sivakumar Uthandi, Sugitha Thankappan, Vijai Kumar Gupta, and Bhim Pratap Singh
- Subjects
Medicine ,Science - Abstract
Endophytic fungi associated with medicinal plants are reported as potent producers of diverse classes of secondary metabolites. In the present study, an endophytic fungi, Aspergillus clavatonanicus strain MJ31, exhibiting significant antimicrobial activity was isolated from roots of Mirabilis jalapa L., was identified by sequencing three nuclear genes i.e. internal transcribed spacers ribosomal RNA (ITS rRNA), 28S ribosomal RNA (28S rRNA) and translation elongation factor 1- alpha (EF 1α). Ethyl acetate extract of strain MJ31displayed significant antimicrobial potential against Bacillus subtilis, followed by Micrococccus luteus and Staphylococcus aureus with minimum inhibitory concentrations (MIC) of 0.078, 0.156 and 0.312 mg/ml respectively. In addition, the strain was evaluated for its ability to synthesize bioactive compounds by the amplification of polyketide synthase (PKS) and non ribosomal peptide synthetase (NRPS) genes. Further, seven antibiotics (miconazole, ketoconazole, fluconazole, ampicillin, streptomycin, chloramphenicol, and rifampicin) were detected and quantified using UPLC-ESI-MS/MS. Additionally, thermal desorption-gas chromatography mass spectrometry (TD-GC-MS) analysis of strain MJ31 showed the presence of 28 volatile compounds. This is the first report on A. clavatonanicus as an endophyte obtained from M. jalapa. We conclude that A. clavatonanicus strain MJ31 has prolific antimicrobial potential against both plant and human pathogens and can be exploited for the discovery of new antimicrobial compounds and could be an alternate source for the production of secondary metabolites.
- Published
- 2017
- Full Text
- View/download PDF
43. Evaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction.
- Author
-
Dhaneshwaree Asem, Vincent Vineeth Leo, Ajit Kumar Passari, Mary Vanlalhruaii Tonsing, J Beslin Joshi, Sivakumar Uthandi, Abeer Hashem, Elsayed Fathi Abd Allah, and Bhim Pratap Singh
- Subjects
Medicine ,Science - Abstract
The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained bacterial isolates, Serratia rubidaea strain DBT4 and Aneurinibacillus aneurinilyticus strain DBT87 were revealed as the most potent strains, showing both cellulase and xylanase production. A biomass utilization study showed that submerged fermentation (SmF) of D2 (alkaline pretreated pulpy biomass) using strain DBT4 resulted in the most efficient biomass deconstruction with maximum xylanase (11.98 U/mL) and FPase (0.5 U/mL) activities (55°C, pH 8). The present study demonstrated that bacterial strains residing in the gastrointestinal region of non-ruminant swine are a promising source for lignocellulose degrading microorganisms that could be used for biomass conversion.
- Published
- 2017
- Full Text
- View/download PDF
44. A new Enzolv process for simultaneous delignification and lignin-derived chemical production from the woody biomass of Melia dubia
- Author
-
Iniya Kumar Muniraj, P. V. Anbu, K. T. Parthiban, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2022
45. Xylitol Production from Corncob Hydrolysate by an Engineered Escherichia coli M15 as Whole-Cell Biocatalysts
- Author
-
Manikandan Ariyan, Sugitha Thankappan, Priyadharshini Ramachandran, and Sivakumar Uthandi
- Subjects
Environmental Engineering ,Renewable Energy, Sustainability and the Environment ,Waste Management and Disposal - Published
- 2022
46. Optimization of combined lime and hydrodynamic cavitation for pretreatment of corncob biomass using response surface methodology for lignin removal
- Author
-
Kiruthika Thangavelu, Ramesh Desikan, and Sivakumar Uthandi
- Subjects
Renewable Energy, Sustainability and the Environment - Published
- 2022
47. Calcite dissolution by Brevibacterium sp. SOTI06: A futuristic approach for the reclamation of calcareous sodic soils
- Author
-
Tamilselvi S.M, Chitdeshwari Thiyagarajan, and Sivakumar Uthandi
- Subjects
calcite dissolution ,calcareous soils ,In-vitro analysis ,Brevibacterium sp. ,sodicity reclamation ,Plant culture ,SB1-1110 - Abstract
Assessing the ability of soil microorganisms to dissolute poorly soluble native calcite to supply Ca2+ is a new area to be explored in reclaiming sodic soils by supplying adequate Ca2+ and reducing the recurrent sodicity. Hence, the present study aimed to isolate a calcite dissolving bacteria (CDB) from calcareous sodic soils and to understand the mechanism of calcite dissolution. Of the thirty three CDB isolates recovered from the calcareous sodic soils of Tamil Nadu (Coimbatore, Ramnad and Trichy), eleven isolates were screened for calcite dissolution based on titratable acidity. 16S rRNA gene sequence analysis of the three best isolates viz., SORI09, SOTI05 and SOTI06 revealed 99 % similarity to Bacillus aryabhattai, 100 % to B. megaterium and 93 % to Brevibacterium sp., respectively. Among them, Brevibacterium sp. SOTI06 released more Ca2+ (3.6 g.l-1) by dissolving 18.6 % of the native calcite. The spectral data of FTIR also showed reduction in the intensity of calcite (55.36 to 41.27) by the isolate at a wave number of 1636 cm-1 which confirmed the dissolution. Besides producing organic acids (gluconic acid and acetic acid), Brevibacterium sp. SOTI06 also produced siderophore (91.6 %) and extracellular polysaccharides (EPS, 13.3 µg. ml-1) which might have enhanced the calcite dissolution.
- Published
- 2016
- Full Text
- View/download PDF
48. Microbial biodiesel production from lignocellulosic biomass: New insights and future challenges
- Author
-
Sivakumar Uthandi, Iniya Kumar Muniraj, Vijai Kumar Gupta, Xinmin Zhan, Naganandhini Srinivasan, Kiruthika Thangavelu, Nicholas Gathergood, and Ashokkumar Kaliyaperumal
- Subjects
Biodiesel ,Animal fat ,Environmental Engineering ,business.industry ,Microorganism ,0208 environmental biotechnology ,food and beverages ,Lignocellulosic biomass ,02 engineering and technology ,010501 environmental sciences ,Raw material ,Pulp and paper industry ,01 natural sciences ,Pollution ,020801 environmental engineering ,Renewable energy ,Biodiesel production ,Environmental science ,Production (economics) ,business ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
In many countries, biodiesel production is obstructed because of a high production cost accounting for raw materials, the large acreage needed for the cultivation of oil-yielding vegetable crops, and competition between food and feed. Therefore, biodiesel production requires new approaches for which microbial oils offer a potential solution. Among several microorganisms available, oleaginous microorganisms (yeast and fungi) accumulate more than 20-70% oils inside their cells when grown in specific environmental conditions. Moreover, microbial oils or single cell oils (SCOs) offer numerous advantages over vegetable oils or animal fats such as similar fatty acid profile, short life cycles of the microbes, relatively lower environmental impact, reduced labor demand, and convenient scalability. Microbial lipids production using lignocellulosic biomass (LCB), which are naturally available in abundance, as a renewable raw material for producing second-generation biodiesel, has become a fundamental approach for tackling the challenges we face of higher energy costs, protection of the environment, and rapid depletion of crude oil reserves. This review compares and examines the extent to which different microbes can accumulate a productive level of lipids using lignocellulosic biomass as substrates, pretreatment strategies used for converting LCB into SCOs, and future challenges in using LCB for biodiesel production.
- Published
- 2021
49. Characterization of biomass produced by Candida tropicalis ASY2 grown using sago processing wastewater for bioenergy applications and its fuel properties
- Author
-
Kiruthika Thangavelu, Naganandhini Srinivasan, Sivakumar Uthandi, and Pugalendhi Sundararaju
- Subjects
Biodiesel ,biology ,Renewable Energy, Sustainability and the Environment ,Chemistry ,020209 energy ,Biomass ,02 engineering and technology ,010501 environmental sciences ,Raw material ,Pulp and paper industry ,biology.organism_classification ,01 natural sciences ,Candida tropicalis ,Biofuel ,Bioenergy ,Biodiesel production ,0202 electrical engineering, electronic engineering, information engineering ,Energy source ,0105 earth and related environmental sciences - Abstract
The cost of biodiesel production and the requirement of raw ingredients are the primary constraints that need to be addressed while searching for viable alternative fuels to petrol and diesel. Oleaginous yeasts are gaining wider acceptance as biofuel candidates among oil-rich crops/microbes. The present investigation aimed to integrate the agro-industrial wastewater stream as a nutrient source for the cultivation of oleaginous yeast and to use the resultant biomass and lipid as a feedstock for biofuel synthesis. The yeast biomass grown in sago processing wastewater contained 7.21% moisture content, 69.01% volatile matter, 12.61% fixed carbon, and 11.16% ash content. The ultimate analysis determined the contents of carbon (40.43%), nitrogen (5.14%), hydrogen (4.62%), sulfur (0.54%), and oxygen (49.27%). The heating value of yeast biomass was 16.54 MJ kg−1. The thermal behavior of yeast biomass also suggests its potential use as an energy source. The FTIR spectrum of biomass had major lipid (3030–2800 cm−1 and 1500–1350 cm−1) and carbohydrate (1250 cm−1 and 1000 cm−1) functional peaks. Further FAME profiling revealed that the yeast biomass is primarily composed of stearic, oleic, linoleic, and linolenic acids, similar to the vegetable oils. The fuel characteristics of yeast biodiesel (SV, 168.87 mg KOH g−1; IV, 120 mg I2 100 g−1; CN, 61.79; and KV, 3.16 mm2 s−1) are also within the ASTM standard limits, suggesting that yeast biomass could be a sustainable and economically viable feedstock for both solid and liquid biofuel production.
- Published
- 2021
50. Physiological Changes during Flower Petal Developmental Stages of Jasminum sambac cv. Ramanathapuram Gundumalli
- Author
-
Sivakumar Uthandi, Shakila Sadasivam M. Jawaharlal, and Haripriya Shanmugam D. Uma
- Subjects
Horticulture ,Petal ,Biology - Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.