135 results on '"Shunichi Kojima"'
Search Results
2. Tongue-palatal contact changes in patients with skeletal mandibular prognathism after sagittal split ramus osteotomy: an electropalatography study
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Kengo Nakajima, Hiromi Sumi, Masato Kaku, Y Nagano, I Yamamoto, Yuka Yashima, M Ueasa, M Kono, Shunichi Kojima, Y Yasuhara, Jin Izumino, Taeko Yamamoto, A Yoshimura, and Kotaro Tanimoto
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Adult ,Male ,Time Factors ,Osteotomy, Sagittal Split Ramus ,Dentistry ,Mandible ,Bite Force ,Young Adult ,030507 speech-language pathology & audiology ,03 medical and health sciences ,Electropalatography ,0302 clinical medicine ,Cog ,Tongue ,Sagittal Split Ramus Osteotomy ,medicine ,Humans ,Prognathism ,030223 otorhinolaryngology ,General Dentistry ,Orthodontics ,business.industry ,Electrodiagnosis ,Proprioception ,medicine.disease ,Bite force quotient ,Treatment Outcome ,medicine.anatomical_structure ,Mandibular prognathism ,Female ,0305 other medical science ,business - Abstract
The purpose of this study was to investigate the changes in tongue-palatal contact patterns using electropalatography (EPG) before and after sagittal split ramus osteotomy (SSRO) in patients with mandibular prognathism. Nine clients who underwent SSRO for mandibular setback and seven control subjects were participated in this study. Tongue-palatal contact patterns for /t/, /s/ and /k/ production were investigated using EPG before surgery and 3 months after surgery. The mean value of whole total of palate contact (WT) in the maximum contact frame was examined before and after SSRO. The correlation quantity between the change of center of gravity (COG) value and the amount of mandibular setback was also evaluated. The mean value of WT for /t/ and /s/ significantly increased after SSRO, and the EPG pattern became normal. However, a remarkable change in WT for /k/ was not observed, and the mean value was significantly larger in the SSRO group before and after surgery than in the control group. A negative correlation between COG variation and the amount of mandibular setback for /t/ and positive correlation for /s/ was observed. This study demonstrated that tongue-palatal contact patterns for /t/ and /s/ articulation improved clearly after SSRO. There was a significant correlation between COG variation and the amount of mandibular setback. However, no significant change was detected through perceptual assessment before and after SSRO. Further investigation is needed to determine whether these results will change over time.
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- 2017
3. Study on Stable Operation of Micro-thruster Using Stacked Solid Propellant Pellets
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Hiroyuki Koizumi, Masakatsu Nakano, Jun Asakawa, and Shunichi Kojima
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Propellant ,020301 aerospace & aeronautics ,Materials science ,0203 mechanical engineering ,0103 physical sciences ,Pellets ,02 engineering and technology ,Composite material ,01 natural sciences ,010305 fluids & plasmas - Published
- 2017
4. Genetic factors associated with elevation of uric acid after treatment with thiazide-like diuretic in patients with essential hypertension
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Shunichi Kojima, Satoko Nakamura, Tomohiro Katsuya, Ken Sugimoto, Takuya Tsuchihashi, Takeshi Horio, Takeshi Nakahashi, Kei Kamide, Yuhei Kawano, Katsuhiro Higashiura, Hajime Nakahama, Toshiyuki Miyata, Kazuaki Shimamoto, Naonaga Hosomi, Hironori Hanada, Norio Komai, Jitsuo Higaki, Yuko Ohta, Johji Kato, Fumiki Yoshihara, Hideo Matsuura, Tetsuro Miki, Michiya Igase, Michio Ueno, Shigeto Morimoto, Tatsuo Shinagawa, Ritsuko Katabuchi, Masayoshi Soma, Kazuhiro Sase, Toshiyuki Sasaguri, Takafumi Oukura, Hiromi Rakugi, Shin Takiuchi, Kazuo Takeda, Yosikazu Miwa, Toshihiko Ishimitsu, and Yoshio Iwashima
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Male ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Renal function ,Blood Pressure ,030204 cardiovascular system & hematology ,Essential hypertension ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,chemistry.chemical_compound ,Angiotensin Receptor Antagonists ,0302 clinical medicine ,Internal medicine ,Internal Medicine ,medicine ,Humans ,030212 general & internal medicine ,Amlodipine ,Diuretics ,Aged ,Cross-Over Studies ,business.industry ,Indapamide ,Middle Aged ,medicine.disease ,Calcium Channel Blockers ,Uric Acid ,Endocrinology ,Blood pressure ,Valsartan ,chemistry ,Uric acid ,Female ,Diuretic ,Essential Hypertension ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug ,Genome-Wide Association Study - Abstract
We investigated changes in blood pressure (BP) and metabolic adverse effects, especially elevation of uric acid (UA), after treatment with a thiazide-like diuretic (TD) in patients with essential hypertension. Furthermore, the role of genetic factors in the elevation of UA by TD was assessed by a 500 K SNP DNA microarray. The subjects included 126 hypertensive patients (57 women and 69 men, mean age 59 ± 12 years) who registered for the GEANE (Gene Evaluation for ANtihypertensive Effects) study. After one month of the nontreatment period, TD, indapamide, angiotensin II receptor antagonist valsartan, and Ca channel blocker amlodipine were administered to all patients for 3 months each in a randomized crossover manner. BP, renal function, serum UA level, and electrolytes were measured at baseline and at the end of each treatment period. Single nucleotide polymorphisms (SNPs) associated with UA elevation after treatment with indapamide were investigated by a genome-wide association study (GWAS). Indapamide significantly decreased both office and home BP levels. Treatment with indapamide also significantly reduced the estimated glomerular filtration rate and serum potassium and increased serum UA. Patients whose UA level increased more than 1 mg/dl showed significantly higher baseline office SBP and plasma glucose and showed greater decline in renal function compared with those who showed less UA increase (
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- 2019
5. The effect of magnetic field during freezing and thawing of rat bone marrow-derived mesenchymal stem cells
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Shunichi Kojima, Takashi Yamamoto, Hanaka Shikata, Masato Kaku, Shotoku Kojima, Toshitsugu Kawata, Kazuo Tanne, Yuka Yashima, Hiromi Sumi, and Kotaro Tanimoto
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Male ,0301 basic medicine ,Bone Regeneration ,Cell Survival ,Calvaria ,Biology ,Rat Bone Marrow ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,03 medical and health sciences ,Freezing ,medicine ,Animals ,Humans ,Bone regeneration ,Survival rate ,030102 biochemistry & molecular biology ,Cell growth ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,General Medicine ,Rats ,Transplantation ,Magnetic Fields ,030104 developmental biology ,medicine.anatomical_structure ,Immunology ,General Agricultural and Biological Sciences ,human activities - Abstract
Previous studies showed that a programmed freezer with magnetic field can maintain a high survival rate of mesenchymal stem cells (MSCs). The purpose of this study was to evaluate the influences of magnetic field during freezing and thawing on the survival of MSCs isolated from rat bone marrow. The cells were frozen by a normal programmed freezer or a programmed freezer with magnetic field (CAS-LAB1) and cryopreserved for 7 days at -150 °C. Then, the cells were thawed in the presence or absence of magnetic field. Immediately after thawing, the number of surviving or viable cells was counted. The cell proliferation was examined after 1-week culture. Cryopreserved MSCs which were frozen by a normal freezer or a CAS freezer were transplanted into bone defects artificially made in calvaria of 4-week-old rats. Non-cryopreserved MSCs were used as a control. The rats were sacrificed at 8, 16, or 24 weeks after transplantation and the bone regeneration area was measured. Proliferation rates of MSCs after 1 week were significantly higher in the CAS-freezing-thawing group than in the CAS-freezing group. The extent of new bone formation in the CAS-freezing-thawing group tended to be larger than in CAS-freezing group 24 weeks after transplantation. These results suggest that a magnetic field enhances cell survival during thawing as well as freezing.
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- 2016
6. Two-Stage Approach for Treatment of Two Patients with Facial Asymmetry Caused by Unilateral Condylar Hyperplasia
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Koutaro Tanimoto, Masato Kaku, Junji Otani, Kazumi Kubozono, Masaaki Takechi, Takayuki Nakagawa, Shunichi Kojima, Shigehiro Ono, Kazuo Tanne, Akemi Sumi, Kouji Ohta, and Ryo Uetsuki
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Orthodontics ,business.industry ,Medicine ,Stage (cooking) ,business ,Facial symmetry ,Unilateral condylar hyperplasia - Published
- 2016
7. Cranial suture-like gap and bone regeneration after transplantation of cryopreserved MSCs by use of a programmed freezer with magnetic field in rats
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Hiromi Sumi, Shotoku Kojima, Masahide Motokawa, Toshitsugu Kawata, Kazuo Tanne, Kotaro Tanimoto, Masato Kaku, T.H. Abonti, Shunichi Kojima, Taeko Yamamoto, and Hanaka Shikata
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Male ,medicine.medical_specialty ,Bone Regeneration ,Biology ,Mesenchymal Stem Cell Transplantation ,Bone tissue ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Cell Line ,Andrology ,Osteogenesis ,medicine ,Animals ,Bone regeneration ,Cells, Cultured ,Cell Proliferation ,Adipogenesis ,Regeneration (biology) ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,Cranial Sutures ,General Medicine ,Rats, Inbred F344 ,Rats ,Surgery ,Transplantation ,Sagittal suture ,Magnetic Fields ,medicine.anatomical_structure ,Bone marrow ,General Agricultural and Biological Sciences - Abstract
Mesenchymal stem cells (MSCs) can be used for regeneration of various organs and tissues. A previous study revealed that cryopreserved MSCs, which were frozen by a programmed freezer with a magnetic field (Cells Alive System: CAS) and cryopreserved for 7 days in a -150°C deep freezer, can maintain high survival and proliferation rates while retaining both adipogenic and osteogenic differentiation abilities. The purpose of this study was to examine MSC viability and tissue regenerative ability after long-term cryopreservation using a CAS freezer. MSCs were isolated from rat femora bone marrow and cryopreserved in a -150°C deep freezer (CAS group) or directly cryopreserved in a deep freezer (Direct group). After 3 years, the cells were thawed and the number of viable cells was counted. Cell proliferation was also examined after 14 days in culture. For histological examination, forty 4-week-old Fischer 344 male rats received bone and sagittal suture defects with a diameter of 6.0mm, and MSCs (CAS or Direct group) cryopreserved for 1 year were grafted with membranes. Non-cryopreserved MSCs (Control group) were transplanted to an additional twenty rats. The rats were sacrificed at 4, 8, 16, and 24 weeks after surgery. The parietal bones, including the sagittal suture, were observed under a light microscope and the extent of bone regeneration was measured. Our results indicate that MSCs survival and proliferation rates were significantly higher in the CAS group than in the Direct group. In the Control and CAS groups, a large amount of new bone formation and a suture-like gap was identified 24 weeks after transplantation, whereas only a small amount of new bone formation was observed in the Direct group. These results suggest that the CAS freezer is amenable to long-term cryopreservation of MSCs, which can be applied to the regeneration of various tissues, including bone tissue with suture-like gap formation.
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- 2015
8. ANALYSIS OF RELIABILITY AND MAINTAINABILITY OF GHP AIR-CONDITIONERS IN UNIVERSITY BUILDINGS
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Hirofumi Hayama, Shunichi Kojima, Taro Mori, and Koki Kikuta
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Engineering ,Environmental Engineering ,Air conditioning ,business.industry ,Maintainability ,Systems engineering ,business ,Reliability (statistics) ,Reliability engineering - Published
- 2015
9. Effects of Pulpectomy on the Amount of Root Resorption during Orthodontic Tooth Movement
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Masahide Motokawa, Hiromi Sumi, Tadashi Fujita, Shunichi Kojima, Hanaka Shikata, Kotaro Tanimoto, Masato Kaku, and Kazuo Tanne
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Adult ,Molar ,medicine.medical_specialty ,Time Factors ,Materials science ,Adolescent ,Tooth Movement Techniques ,medicine.medical_treatment ,Root canal ,Interleukin-1beta ,Pulpectomy ,Cell Culture Techniques ,Root Resorption ,Dentistry ,Gadolinium ,Mechanotransduction, Cellular ,Proinflammatory cytokine ,Young Adult ,stomatognathic system ,Internal medicine ,medicine ,Animals ,Humans ,Large-Conductance Calcium-Activated Potassium Channels ,Rats, Wistar ,General Dentistry ,Cells, Cultured ,Dental Pulp ,Tooth, Nonvital ,Tumor Necrosis Factor-alpha ,business.industry ,Macrophage Colony-Stimulating Factor ,RANK Ligand ,Biomechanical Phenomena ,Rats ,stomatognathic diseases ,Cytokine ,Endocrinology ,medicine.anatomical_structure ,Vacuolization ,Pulp (tooth) ,Tumor necrosis factor alpha ,Stress, Mechanical ,business - Abstract
Previous studies have revealed that orthodontic force affects dental pulp via the rupture of blood vessels and vacuolization of pulp tissues. We hypothesized that pulp tissues express inflammatory cytokines and regulators of odontoclast differentiation after excess orthodontic force. The purpose of this study was to investigate the effects of tensile force in human pulp cells and to measure inflammatory root resorption during tooth movement in pulpless rat teeth.After cyclic tensile force application in human pulp cells, gene expression and protein concentration of macrophage colony-stimulating factor, receptor activator of nuclear factor kappa-B ligand, interleukin-1 beta, and tumor necrosis factor alpha were determined by real-time polymerase chain reaction and enzyme-linked immunoassay. Moreover, the role of the stretch-activated channel was evaluated by gadolinium (Gd(3+)) treatment. The upper right first molars of 7-week Wistar rats were subjected to pulpectomy and root canal filling followed by mesial movement for 6 months.The expression of cytokine messenger RNAs and proteins in the experimental group peaked with loading at 10-kPa tensile force after 48 hours (P.01). Gd(3+) reduced the expression of these cytokine messenger RNAs and protein concentrations (P.01). The amount of inflammatory root resorption was significantly larger in the control teeth than the pulpectomized teeth (P.05).This study shows that tensile forces in the pulp cells enhance the expression of various cytokines via the S-A channel, which may lead to inflammatory root resorption during tooth movement. It also suggests that root canal treatment is effective for progressive severe inflammatory root resorption during tooth movement.
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- 2014
10. Irradiation effects of low temperature multi gas plasma jet on oral bacteria
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Hidekazu Miyahara, Kotaro Tanimoto, Masato Kaku, Taeko Yamamoto, Kazuo Tanne, Shotoku Kojima, Akitoshi Okino, Yuka Yashima, Shunichi Kojima, Toshitsugu Kawata, Hiromi Sumi, and Tahsin Raquib Abonti
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Materials science ,Lactobacillus fermentum ,Dental Plaque ,Dental Caries ,Dental plaque ,01 natural sciences ,010305 fluids & plasmas ,Streptococcus mutans ,stomatognathic system ,0103 physical sciences ,medicine ,Humans ,Irradiation ,Dental Enamel ,General Dentistry ,010302 applied physics ,biology ,Enamel paint ,Radiochemistry ,Aggregatibacter actinomycetemcomitans ,Temperature ,Tooth surface ,Sterilization (microbiology) ,biology.organism_classification ,medicine.disease ,stomatognathic diseases ,visual_art ,Ceramics and Composites ,visual_art.visual_art_medium ,Lasers, Gas ,Microscopy, Electron, Scanning ,Nuclear chemistry - Abstract
The purpose of this study is to evaluate the sterilization effects of a newly developed low temperature multi gas plasma jet on oral pathogenic microorganisms (Streptococcus mutans [S. mutans], Lactobacillus fermentum [L. fermentum], Aggregatibacter actinomycetemcomitans [A. actinomycetemcomitans]). Plasma gas which generated from O2, N2, Ar and 50% (O2+N2) was irradiated to the microbes. Effect of O2 plasma irradiation on S. mutans under scanning electron microscopy (SEM) was also observed. O2 plasma was directly applied to dental plaque on human extracted tooth. Then, the depth of enamel resorption area was noted by nanoscale hybrid microscope. O2 had the best sterilizing effect for all microbes. The potent bactericidal effect of plasma irradiation was also observed by SEM. Decalcification of enamel was noted significantly lower in plasma irradiated tooth surface compared to no plasma exposure group. These findings revealed that multi gas plasma jet has great potential to be used for dental treatment.
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- 2016
11. Genome-wide response to antihypertensive medication using home blood pressure measurements: a pilot study nested within the HOMED-BP study
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Kei, Kamide, Kei, Asayama, Tomohiro, Katsuya, Takayoshi, Ohkubo, Takuo, Hirose, Ryusuke, Inoue, Hirohito, Metoki, Masahiro, Kikuya, Taku, Obara, Hironori, Hanada, Lutgarde, Thijs, Tatiana, Kuznetsova, Yuichi, Noguchi, Ken, Sugimoto, Mitsuru, Ohishi, Shigeto, Morimoto, Takeshi, Nakahashi, Shin, Takiuchi, Toshihiko, Ishimitsu, Takuya, Tsuchihashi, Masayoshi, Soma, Jitsuo, Higaki, Hideo, Matsuura, Tatsuo, Shinagawa, Toshiyuki, Sasaguri, Tetsuro, Miki, Kazuo, Takeda, Kazuaki, Shimamoto, Michio, Ueno, Naohisa, Hosomi, Jyouji, Kato, Norio, Komai, Shunichi, Kojima, Kazuhiro, Sase, Toshiyuki, Miyata, Hitonobu, Tomoike, Yuhei, Kawano, Toshio, Ogihara, Hiromi, Rakugi, Jan A, Staessen, Yutaka, Imai, A, De Soete, Epidemiologie, and RS: CARIM School for Cardiovascular Diseases
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Male ,Angiotensin receptor ,Randomization ,Genotyping Techniques ,medicine.drug_class ,n genome wide association study ,Angiotensin-Converting Enzyme Inhibitors ,Pilot Projects ,Calcium channel blocker ,030204 cardiovascular system & hematology ,Pharmacology ,Essential hypertension ,Biomarkers, Pharmacological ,03 medical and health sciences ,Angiotensin Receptor Antagonists ,0302 clinical medicine ,home blood pressure ,Genetics ,medicine ,Humans ,Amlodipine ,Precision Medicine ,Genotyping ,blood pressure response ,Antihypertensive Agents ,Aged ,Randomized Controlled Trials as Topic ,business.industry ,Blood Pressure Monitoring, Ambulatory ,Middle Aged ,medicine.disease ,Calcium Channel Blockers ,personalized treatment ,3. Good health ,Blood pressure ,Valsartan ,antihypertensive drugs ,Hypertension ,Molecular Medicine ,Female ,business ,030217 neurology & neurosurgery ,medicine.drug ,Genome-Wide Association Study - Abstract
Background: Patients with mild-to-moderate essential hypertension in the HOMED-BP trial were randomly allocated to first-line treatment with a calcium channel blocker (CCB), angiotensin-converting enzyme inhibitor (ACEI) or angiotensin II receptor blocker (ARB). Methods: We recruited 265 (93 for CCB, 71 for ACEI and 101 for ARB) patients who completed the genomic study. Home blood pressure was measured for 5 days off-treatment before randomization and for 5 days after 2-4 weeks of randomized drug treatment. Genotyping was performed by 500K DNA microarray chips. The blood pressure responses to the three drugs were analyzed separately as a quantitative trait. For replication of SNPs with p < 10(-4), we used the multicenter GEANE study, in which patients were randomized to valsartan or amlodipine. Results: SNPs in PICALM, TANC2, NUMA1 and APCDD1 were found to be associated with CCB responses and those in ABCC9 and YIPF1 were found to be associated with ARB response with replication. Conclusion: Our approach, the first based on high-fidelity phenotyping by home blood pressure measurement, might be a step in moving towards the personalized treatment of hypertension. Original submitted 29 April 2013; Revision submitted 14 August 2013. ispartof: Pharmacogenomics vol:14 issue:14 pages:1709-21 ispartof: location:England status: published
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- 2013
12. Gummy smile and facial profile correction using miniscrew anchorage
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Masato Kaku, Hiroyuki Koseki, Junji Ohtani, Tadashi Fujita, Shunichi Kojima, Masahide Motokawa, Toshitsugu Kawata, Kazuo Tanne, Hiromi Sumi, and Sara Abedini
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Orthodontics ,Alternative methods ,business.industry ,Facial profile ,Dentistry ,Skeletal class ,medicine.disease ,Surgical therapy ,Gummy smile ,medicine ,Active treatment ,Malocclusion ,Surgical treatment ,business - Abstract
This case report describes the treatment of a case involving a skeletal Class II facial profile with a gummy smile. While treating a facial profile and a gummy smile, the outcome may not always be successful with orthodontic therapy alone. For this reason, surgical therapy is often chosen to gain an esthetic facial profile and a good smile. However, sometimes the patients reject surgical treatment and an alternative method must be considered. Skeletal anchorage systems such as miniscrews are now frequently used for correcting severe malocclusion that should be treated by surgical therapy. In this case report, we treated a skeletal Class II malocclusion with a convex profile and a gummy smile using miniscrews, which were placed in the upper posterior and anterior areas. The active treatment period was 3.5 years, and the patient's teeth continued to be stable after a retention period of 36 months.
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- 2011
13. Treatment of a Case of Skeletal Class II Malocclusion with Temporomandibular Joint Disorder Using Miniscrew Anchorage
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Aki Kawazoe, Hiroyuki Koseki, Masahide Motokawa, Sara Abedini, Toshitsugu Kawata, Junji Ohtani, Kazuo Tanne, Shunichi Kojima, Tadashi Fujita, and Masato Kaku
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Rotation ,Tooth Movement Techniques ,Overjet ,Bone Screws ,Joint Dislocations ,Dentistry ,Mandible ,Malocclusion, Angle Class II ,Overbite ,Mandibular first molar ,Condyle ,Young Adult ,stomatognathic system ,Temporomandibular Joint Disc ,Orthodontic Anchorage Procedures ,medicine ,Temporomandibular Joint Disorder ,Humans ,General Dentistry ,Orthodontics ,Temporomandibular Joint ,business.industry ,Mandibular Condyle ,Open Bite ,Temporomandibular Joint Disorders ,medicine.disease ,Molar ,Temporomandibular joint ,stomatognathic diseases ,Treatment Outcome ,medicine.anatomical_structure ,Otorhinolaryngology ,Posterior teeth ,Female ,Malocclusion ,business - Abstract
At the present time, there are no reports in the literature on the treatment of temporomandibular joint disorder (TMD) by intrusion of molars using mini-screws. This case report describes the treatment for a female patient, aged 19 years seven months, with a TMD and an excessive lower anterior facial height. Overjet and overbite were +5.0 mm and +0.5 mm, respectively. The patient had a history of orthodontic treatment in which her first premolars were all extracted. During the first orthodontic treatment, a clockwise mandibular rotation was observed as a result of the increase of posterior dentoalveolar height. She had temporomandibular joint (TMJ) pain during mouth opening and complained of difficulty in eating due to masticatory dysfunction. The pretreatment Schuller views of both TMJ showed a posterior condyle position. In order to correct the overjet, molar relationship and the mandibular condyle position, a miniscrew was inserted into the palatal region of the upper first molar to intrude the upper posterior teeth. As the upper molars were intruded, the overjet was decreased, and a class I molar relationship was achieved by a counterclockwise mandibular rotation. After one year of treatment, an acceptable occlusion was achieved, and the condyle moved into centric position in the glenoid fossa. The patient's teeth continued to be stable, and she had no pain in TMJ after a retention period of three years. The result of this treatment showed that molar intrusion using miniscrew anchorage is effective for treatment of a TMD patient with a posterior condyle position.
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- 2011
14. Cryopreservation of periodontal ligament cells with magnetic field for tooth banking
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N. Ohwada, Hiroko Kamada, Masato Kaku, Junji Ohtani, Sara Abedini, Toshitsugu Kawata, Natsumi Tsuka, Yayoi Matsuda, Shunichi Kojima, Hiroyuki Koseki, R.A.M. Hernandes, Hiroko Sunagawa, Kazuo Tanne, Tsuyoshi Fujita, and Masahide Motokawa
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Cryobiology ,Chemistry ,business.industry ,Dimethyl sulfoxide ,Dentistry ,General Medicine ,Tooth autotransplantation ,equipment and supplies ,Organ culture ,medicine.disease ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,stomatognathic diseases ,chemistry.chemical_compound ,stomatognathic system ,medicine ,Periodontal fiber ,General Agricultural and Biological Sciences ,business ,human activities ,Cell damage ,Histological examination - Abstract
The purpose of this study was to establish a long-term tooth cryopreservation method that can be used for tooth autotransplantation. Human periodontal ligament (PDL) cells were frozen in 10% dimethyl sulfoxide (Me(2)SO) using a programmed freezer with a magnetic field. Cells were cryopreserved for 7 days at -150 degrees C. Immediately after thawing, the number of surviving cells was counted and the cells were cultured; cultured cells were examined after 48 h. Results indicated that a 0.01 mT of a magnetic field, a 15-min hold-time, and a plunging temperature of -30 degrees C led to the greatest survival rate of PDL cells. Based on these findings, whole teeth were cryopreserved under the same conditions for 1 year. The organ culture revealed that the PDL cells of cryopreserved tooth with a magnetic field could proliferate as much as a fresh tooth, although the cells did not appear in the cryopreserved tooth without a magnetic field. Histological examination and the transmission electron microscopic image of cryopreserved tooth with a magnetic field did not show any destruction of cryopreserved cells. In contrast, severe cell damage was seen in cells frozen without a magnetic field. These results indicated that a magnetic field programmed freezer is available for tooth cryopreservation.
- Published
- 2010
15. A case of tooth autotransplantation after long-term cryopreservation using a programmed freezer with a magnetic field
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Kotaro Tanimoto, Kazuo Tanne, Hanaka Shikata, Shotoku Kojima, Masahide Motokawa, Junji Ohtani, Shunichi Kojima, Hiroshi Shimasue, Masato Kaku, Hiromi Sumi, Tahsin Raquib Abonti, and Toshitsugu Kawata
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medicine.medical_treatment ,Magnetic Field Therapy ,Osteotomy, Sagittal Split Ramus ,Dentistry ,Orthodontics ,Case Reports ,Osteotomy ,Mandibular first molar ,Patient Care Planning ,Young Adult ,stomatognathic system ,Occlusion ,Premolar ,medicine ,Humans ,Osteotomy, Le Fort ,Bicuspid ,Tooth Socket ,Autografts ,Cryopreservation ,Radicular Cyst ,business.industry ,Mandible ,Open Bite ,Periodontium ,medicine.disease ,Molar ,Autotransplantation ,Root Canal Therapy ,stomatognathic diseases ,medicine.anatomical_structure ,Malocclusion, Angle Class III ,Treatment Outcome ,Tooth Extraction ,Female ,Malocclusion ,business ,Periapical Periodontitis ,Follow-Up Studies - Abstract
This case report describes the treatment of a skeletal Class III malocclusion with autotransplantation of a cryopreserved tooth. To gain an esthetic facial profile and good occlusion, extraction of bimaxillary premolars and surgical therapy were chosen. The patient had chronic apical periodontitis on the lower left first molar. Although she did not feel any pain in that region, the tooth was considered to have a poor prognosis. Therefore, we cryopreserved the extracted premolars to prepare for autotransplantation in the lower first molar area because the tooth would probably need to be removed in the future. The teeth were frozen by a programmed freezer with a magnetic field (CAS freezer) that was developed for tissue cryopreservation and were cryopreserved in −150°C deep freezer. After 1.5 years of presurgical orthodontic treatment, bilateral sagittal split ramus osteotomy was performed for mandible setback. Improvement of the facial profile and the occlusion were achieved in the retention phase. Six years after the initial visit, the patient had pain on the lower left first molar, and discharge of pus was observed, so we extracted the lower left first molar and autotransplanted the cryopreserved premolar. Three years later, healthy periodontium was observed at the autotransplanted tooth. This case report suggests that long-term cryopreservation of teeth by a CAS freezer is useful for later autotransplantation, and this can be a viable technique to replace missing teeth.
- Published
- 2015
16. Dextran Sulfate Adsorption as a Potent Tool for the Treatment of Refractory Hypercholesterolemia
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Shunichi Kojima, Bunichiro Kishino, Nobuhiko Koga, and Akira Yamamoto
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medicine.medical_specialty ,Adsorption ,Dextran sulfate ,Refractory ,Chemistry ,medicine ,Pharmacology ,Surgery - Published
- 2015
17. Plasmapheresis for Prevention and Regression of Coronary Atherosclerosis
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Yasutsugu Toyota, Mariko Harada-Shiba, Motoo Tsushima, Makoto Takamiya, Shunichi Kojima, Nobuhiko Koga, Bunichiro Kishino, Akira Yamamoto, and Ryozo Tatami
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Adult ,Male ,Heterozygote ,medicine.medical_specialty ,Adolescent ,Arteriosclerosis ,medicine.medical_treatment ,Hypercholesterolemia ,MEDLINE ,Coronary Artery Disease ,General Biochemistry, Genetics and Molecular Biology ,Hyperlipoproteinemia Type II ,History and Philosophy of Science ,Internal medicine ,medicine ,Humans ,Child ,Coronary atherosclerosis ,business.industry ,General Neuroscience ,Homozygote ,Plasmapheresis ,Middle Aged ,Lipoproteins, LDL ,Clinical trial ,Multicenter study ,Child, Preschool ,Female ,business - Published
- 2006
18. Changes in C-reactive Protein Plasma Levels During Low-density Lipoprotein Apheresis
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Mikio Shida, Shunichi Kojima, and Hiroyuki Yokoyama
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Adult ,Male ,medicine.medical_specialty ,Hypercholesterolemia ,Sensitivity and Specificity ,Severity of Illness Index ,Cohort Studies ,Internal medicine ,Severity of illness ,medicine ,Humans ,Risk factor ,Interleukin 6 ,Aged ,biology ,Interleukin-6 ,business.industry ,C-reactive protein ,Interleukin ,Plasmapheresis ,Hematology ,Middle Aged ,Prognosis ,Lipoproteins, LDL ,C-Reactive Protein ,Treatment Outcome ,Endocrinology ,Apheresis ,Nephrology ,LDL apheresis ,biology.protein ,Female ,business ,Biomarkers ,Lipoprotein - Abstract
C-reactive protein (CRP) is one of the important risk factors for atherosclerosis, and its serum level is lowered by popular cholesterol-lowering drugs, statins. This study was undertaken to examine the changes of CRP levels during dextran-sulfate (DS) low-density lipoprotein (LDL) apheresis. In 15 apheresis sessions in seven patients with severe hypercholesterolemia (four men and three women, aged between 36 and 70 years), changes in CRP levels were examined. The efficiency in adsorption of CRP with DS column was evaluated by measuring CRP levels in both pre- and post-column plasma. In one patient, the effect of repeated apheresis sessions on CRP preapheresis levels was examined. The changes in interleukin (IL)-6 plasma levels were also examined in six sessions. Although IL-6 levels after 3,000 mL-plasma treatment rose to 170% of preapheresis levels, CRP levels decreased significantly (from 1.91 +/- 0.49 mg/L to 0.89 +/- 0.24, P < 0.01). C-reactive protein was almost completely adsorbed by the DS column and CRP preapheresis levels were decreased gradually by repetition of apheresis. CRP, a novel risk factor of atherosclerosis, was effectively removed by DS-LDL apheresis. The decrease in CRP plasma levels may be involved in prevention of atherosclerotic vascular diseases due to DS-LDL apheresis.
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- 2003
19. Does stent design affect probability of restenosis? A randomized trial comparing Multilink stents with GFX stents
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Kyo-e Tanaka, Shunichi Kojima, Michiko Endo, Michiko Yano, Yuji Matsumoto, Masao Saotome, Toshihiko Sugi, Yuji Yoshitomi, and Morio Kuramochi
- Subjects
Male ,Neointima ,medicine.medical_specialty ,Randomization ,Intimal hyperplasia ,medicine.medical_treatment ,Coronary Disease ,law.invention ,Coronary artery disease ,Randomized controlled trial ,Restenosis ,Recurrence ,law ,Internal medicine ,medicine ,Humans ,Single-Blind Method ,cardiovascular diseases ,Aged ,Hyperplasia ,medicine.diagnostic_test ,business.industry ,Cardiovascular Surgical Procedures ,Stent ,Equipment Design ,Middle Aged ,equipment and supplies ,medicine.disease ,Treatment Outcome ,surgical procedures, operative ,Angiography ,Cardiology ,Female ,Stents ,Radiology ,Cardiology and Cardiovascular Medicine ,business - Abstract
Experimental studies have revealed that stent configuration influences intimal hyperplasia. The purpose of this study was to evaluate clinical outcomes for 2 stent designs in a randomized trial with quantitative coronary angiography (QCA) and intravascular ultrasonography (IVUS).We randomly assigned 100 patients with 107 lesions and symptomatic coronary artery disease to deployment of a Multilink stent (Advanced Cardiovascular Systems, Guidant, Santa Clara, Calif) or a GFX stent (Applied Vascular Engineering, Santa Rosa, Calif) with IVUS guidance. QCA and IVUS studies were performed before and after intervention and at follow-up (4.2 +/- 1.0 months).There were no significant differences in baseline characteristics and QCA and IVUS parameters before and after intervention between the 2 groups. However, minimal lumen diameter at follow-up was significantly larger in the Multilink group (2.46 +/- 0.59 vs 2.08 +/- 0.79 mm, P.05). Maximal in-stent intimal hyperplasia was significantly larger in the GFX group (2.9 +/- 1.7 vs 1.8 +/- 1.2 mm(2), P.01). The restenosis rate differed between the 2 groups (Multilink 4% vs GFX 26%, P =.003). In multiple stepwise logistic regression analysis, the only predictor that significantly correlated with restenosis was stent type (P.01). The odds ratio for the GFX stent-treated vessels was 18.65 (95% confidence interval 2.10-165.45).With deployment of the GFX stent, a thicker neointima develops within the stent. Stent configuration may affect clinical outcomes.
- Published
- 2001
20. Low-Density Lipoprotein Apheresis and Changes in Plasma Components
- Author
-
Shunichi Kojima
- Subjects
medicine.medical_specialty ,Angiotensin-Converting Enzyme Inhibitors ,Coagulation Factor XII ,Bradykinin ,Von Willebrand factor ,Internal medicine ,Humans ,Medicine ,Plasma Volume ,Blood Coagulation ,Kininogen ,biology ,business.industry ,Dextran Sulfate ,Prekallikrein ,Plasmapheresis ,Hematology ,Lipoprotein(a) ,Lipoproteins, LDL ,Endocrinology ,Coagulation ,Biochemistry ,Nephrology ,LDL apheresis ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Adsorption ,business ,Lipoprotein - Abstract
Several different techniques of low-density lipoprotein (LDL) apheresis are available for management of severe hypercholesterolemia. Among them, the adsorption system with a dextran-sulfate cellulose (DSC) column is most widely used. In addition to adsorption of LDL, DSC adsorbs plasma constituents that have the following characteristics: proteins containing apolipoprotein B (Lp[a]); proteins involved in the initial contact phase of the intrinsic coagulation pathway (coagulation factor XII, high-molecular-weight kininogen and prekallikrein); factors with lipophilic characteristics (coagulation factor VII, coagulation factor VIII, and vitamin E); and proteins with adhesive or other characteristics (von Willebrand factor, fibronectin, serum amyloid P component, hepatocyte growth factor). The adsorption of these proteins seems to ameliorate prevention or regression of atherosclerosis. Moreover, plasma treatment by the DSC column may be useful for treatment of inexorable diseases, such as amyloidosis. On the other hand, the DSC column generates bradykinin by activation of the initial contact phase of the intrinsic coagulation pathway. Bradykinin generation may explain the functional improvement in the circulatory system, as well as hypotension during LDL apheresis, which is observed in patients taking ACE inhibitors.
- Published
- 2001
21. Dilated Cardiomyopathy of Becker-Type Muscular Dystrophy with Exon 4 Deletion
- Author
-
Shunichi Kojima, Yuji Yoshitomi, Masao Saotome, and Morio Kuramochi
- Subjects
Cardiomyopathy, Dilated ,Male ,medicine.medical_specialty ,Heart disease ,030204 cardiovascular system & hematology ,Polymerase Chain Reaction ,Dystrophin ,03 medical and health sciences ,0302 clinical medicine ,Hypokinesia ,Internal medicine ,Humans ,Medicine ,030212 general & internal medicine ,Muscular dystrophy ,Muscle, Skeletal ,Ejection fraction ,biology ,business.industry ,Muscle, Smooth ,Dilated cardiomyopathy ,Exons ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Pathophysiology ,Muscular Dystrophy, Duchenne ,Endocrinology ,biology.protein ,Cardiology ,Chromosome Deletion ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Immunostaining - Abstract
The authors report a 47-year-old man with Becker-type muscular dystrophy presenting with dilated cardiomyopathy. Left ventriculography showed diffuse severe hypokinesia: left ventric ular end-diastolic volume index 193 mL/m2, left ventricular end-systolic volume index 143 mL/m 2, and left ventricular ejection fraction 26%. Skeletal muscle biopsy demonstrated a dystrophic process. Genetic analysis revealed a deletion of exon 4. There was a difference in immunos taining pattern between skeletal muscles and cardiac muscles. Severe cardiac dysfunction in this case may be associated with the damage in dystrophin-deficient fibers.
- Published
- 2001
22. Acute Myocardial Infarction in a Discrete Coronary Artery Aneurysm Without Obstruction
- Author
-
Toshihiko Sugi, Yuji Yoshitomi, Michiko Yano, Yuji Matsumoto, Shunichi Kojima, and Morio Kuramochi
- Subjects
Coronary artery aneurysm ,medicine.medical_specialty ,Unusual case ,business.industry ,medicine.medical_treatment ,Electrocardiography in myocardial infarction ,Thrombolysis ,medicine.disease ,Aneurysm ,Internal medicine ,Angioplasty ,medicine ,Cardiology ,Ergonovine ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Myocardial infarction ,Cardiology and Cardiovascular Medicine ,business - Abstract
We report an unusual case of acute myocardial infarction attributable to obstruction of a discrete coronary aneurysm in a 54-year-old man. Although coronary angioplasty and thrombolysis were unsuccessful, serial arteriography showed spontaneous recanalization, and no spasm was induced by ergonovine. We discuss its mechanism.
- Published
- 2000
23. Heterogeneity of renal cortical circulation in hypertension assessed by dynamic computed tomography
- Author
-
Morio Kuramochi, Masao Saotome, Michiko Yano, Yuji Yoshitomi, Shunichi Kojima, Michiko Endo, and Kyo-e Tanaka
- Subjects
Adult ,Male ,medicine.medical_specialty ,Pathology ,Hypertension, Renal ,Kidney Cortex ,Renal cortex ,Coefficient of variation ,Hemodynamics ,Blood volume ,Renal Circulation ,chemistry.chemical_compound ,Hounsfield scale ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Aged ,Analysis of Variance ,Kidney ,Creatinine ,Nephrosclerosis ,Renal circulation ,business.industry ,Middle Aged ,medicine.anatomical_structure ,chemistry ,Cardiology ,Regression Analysis ,Female ,Tomography, X-Ray Computed ,business - Abstract
The aim of this study was to assess the grade of heterogeneous disturbance in the renal cortical circulation using dynamic computed tomography and to investigate the relationship between the heterogeneity of renal cortical circulation and hypertension. We studied 125 patients who underwent dynamic computed tomography (CT) for various abdominal diseases and had no serious hemodynamic abnormalities. In dynamic computed tomography under appropriate conditions, each pixel (image element), less than 1 mm 2 , has a CT number that is in proportion to the concentration of contrast media, which reflects the blood volume in the pixel. The image was constructed at the hilus level about 50 s after the start of a continuous infusion of contrast medium. The mean and standard deviation were calculated from the CT numbers in the renal cortex. The coefficient of variation, ie, the standard deviation divided by the mean value, was used as the index of the heterogeneity of renal cortical circulation. The coefficient of variation was significantly ( P .001) greater in the hypertensive patients (n = 48, 0.174 ± 0.006 [mean ± SE]) than in normotensive subjects (n = 77, 0.140 ± 0.004). The coefficient increased in parallel with the patient’s age and with the grade of renal surface irregularity. In the patients whose serum creatinine levels were normal, this parameter also had a significant relationship (r = 0.367, P .0001) with serum creatinine. These results suggest that the heterogeneity of renal cortical circulation is increased in hypertension and is also associated with aging. This parameter may become a sensitive indicator to detect slight deterioration in the renal cortical circulation.
- Published
- 2000
24. Interaction of alcohol and an 1-blocker on ambulatory blood pressure in patients with essential hypertension
- Author
-
Hitoshi Abe, Shuichi Takishita, Yuhei Kawano, Shunichi Kojima, and Teruo Omae
- Subjects
Adult ,Male ,medicine.medical_specialty ,Ambulatory blood pressure ,Systole ,Blood Pressure ,Essential hypertension ,Plasma renin activity ,Norepinephrine ,Diastole ,Heart Rate ,Internal medicine ,Renin ,Internal Medicine ,Prazosin ,Humans ,Medicine ,Ingestion ,Drug Interactions ,Adrenergic alpha-Antagonists ,Antihypertensive Agents ,Aged ,Ethanol ,business.industry ,Central Nervous System Depressants ,Blood Pressure Monitoring, Ambulatory ,Middle Aged ,medicine.disease ,Circadian Rhythm ,medicine.anatomical_structure ,Endocrinology ,Blood pressure ,Hypertension ,Potassium ,Vascular resistance ,Alpha blocker ,business ,medicine.drug - Abstract
Ingestion of alcohol acutely decreases vascular resistance and blood pressure (BP) with activation of the sympathetic nervous system in Orientals. Although alpha1-blockers are widely used in the treatment of hypertension, the possible interaction between alcohol and alpha1-blockers has not been clarified. We examined the effects of prazosin on the alcohol-induced BP changes in Japanese men with mild hypertension. Ten hypertensive patients (54 +/- 3 years, mean +/- SE) were given 1 mL/kg of alcohol or isocaloric control drink with a light meal in the evening before and 5 to 7 days after treatment with prazosin (1 mg three times daily). Ambulatory BP monitoring was carried out every 30 min for 24 h in each period using Colin ABPM-630. Blood samples were obtained before and 2 h after intake of alcohol or control drink. Before prazosin treatment, alcohol ingestion decreased BP for several hours with a significant reduction in average 24-h BP, whereas it increased heart rate, plasma norepinephrine, and plasma renin activity. Treatment with prazosin caused a significant decrease in 24-h BP (136.3 +/- 4.0/82.8 +/- 2.5 v 131.6 +/- 3.2/80.0 +/- 2.3 mm Hg). The alcohol-induced hypotension at 2-4 h after ingestion was enhanced by prazosin (-18.0 +/- 3.7/-11.8 +/- 2.7 v -24.4 +/- 4.9/-17.8 +/- 2.8 mm Hg, P < .05 for diastolic BP). These results suggested that inhibition of the sympathetic nervous system with alpha1-blockers accentuates alcohol-induced hypotension. Ingestion of alcohol may cause a marked BP reduction in hypertensive Orientals treated with alpha1-blockers.
- Published
- 2000
25. Coronary Artery Spasm Induced by Ergonovine in an Infarct Related Coronary Artery Late After Primary Angioplasty
- Author
-
Morio Kuramochi, Yuji Matsumoto, Toshihiko Sugi, Michiko Yano, Yuji Yoshitomi, and Shunichi Kojima
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Primary angioplasty ,medicine.disease ,Chest pain ,Coronary arteries ,surgical procedures, operative ,medicine.anatomical_structure ,Restenosis ,Internal medicine ,Angioplasty ,medicine ,Cardiology ,Ergonovine ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Myocardial infarction ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Artery - Abstract
We report two cases with induced coronary spasm of the culprit lesion in the infarct related coronary artery in the chronic phase after primary percutaneous transluminal coronary angioplasty (PTCA). To our knowledge, there are few data on the response of the angioplasty site to ergonovine in human coronary arteries after primary PTCA. It is important to note that chest pain recurring after successful primary PTCA is not automatically related to restenosis, and coronary spasm in patients with previous myocardial infarction, should also be considered. (J Interven Cardiol 2000;31–34)
- Published
- 2000
26. Relation between stent expansion and arterial remodeling: A serial intravascular ultrasound study
- Author
-
Morio Kuramochi, Toshihiko Sugi, Shunichi Kojima, Michiko Endo, Yuji Matsumoto, Kyo-e Tanaka, Michiko Yano, Yuji Yoshitomi, and Masao Saotome
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Coronary Disease ,Coronary Angiography ,Intravascular ultrasound ,medicine ,Humans ,Stent implantation ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Myocardial infarction ,Ultrasonography, Interventional ,Aged ,medicine.diagnostic_test ,business.industry ,Stent ,Arteries ,General Medicine ,Middle Aged ,equipment and supplies ,medicine.disease ,surgical procedures, operative ,Female ,Stents ,Radiology ,Cardiology and Cardiovascular Medicine ,business ,Lesion site - Abstract
Previous studies suggested that stent area (SA) did not change after the Palmaz-Schatz stent implantation. Whether these findings apply to other types of stent, however, is unknown. This study assessed vascular response to stent implantation using intravascular ultrasound (IVUS) studies. Serial (pre-intervention to follow-up) IVUS imagings were used to study 57 native coronary lesions after the GFX stent or the Multilink stent implantation. The vessel area (VA) at lesion site increased at follow-up (16.92 +/- 3.67 mm(2) after intervention to 18.17 +/- 4.66 mm(2) at follow-up, P < 0.01). The SA also increased from 8.39 +/- 1.90 mm(2) after intervention to 8.80 +/- 2.08 mm(2) at follow-up (P = 0.02). Thirty-two percent of lesions showed late stent expansion. The stent expansion [Delta (after intervention to follow-up) SA] correlated significantly with the VA growth [Delta (after intervention to follow-up) VA] (r = 0.59, P < 0.0001). In conclusion, some lesions reveal late stent expansion after both the GFX stent and the Multilink stent implantation. Adaptive vessel remodeling may be followed by stent expansion.
- Published
- 2000
27. Serum Hepatocyte Growth Factor in Patients with Peripheral Arterial Occlusive Disease
- Author
-
Yuji Matsumoto, Yuji Yoshitomi, Morio Kuramochi, Shunichi Kojima, Michiko Yano, Toshihiko Sugi, Takuya Umemoto, and Kiyokage Kubo
- Subjects
Adult ,Male ,medicine.medical_specialty ,Pathology ,Angiogenesis ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Clinical Biochemistry ,Arterial Occlusive Diseases ,Biochemistry ,Gastroenterology ,Endocrinology ,Reference Values ,Risk Factors ,Internal medicine ,Angioplasty ,Blood plasma ,medicine ,Humans ,Aged ,Aged, 80 and over ,Peripheral Vascular Diseases ,Kidney ,Hepatocyte Growth Factor ,Vascular disease ,business.industry ,Smoking ,Biochemistry (medical) ,Angiography ,Middle Aged ,medicine.disease ,Pathophysiology ,medicine.anatomical_structure ,Female ,Hepatocyte growth factor ,Wound healing ,business ,Vascular Surgical Procedures ,Angioplasty, Balloon ,medicine.drug - Abstract
Hepatocyte growth factor (HGF) is a multifunctional protein implicated in tissue regeneration, wound healing, and angiogenesis. We measured serum HGF concentrations in 37 patients with peripheral arterial occlusive disease (PAOD). Among them, 36 patients underwent arteriography. Serum HGF concentrations were also measured in 40 control subjects who remained free of vascular, liver, kidney, or lung disease. Patients with PAOD showed elevated serum HGF concentrations compared with control subjects (0.40+/-0.02 vs. 0.19+/-0.01 ng/mL; P0.001). Serum HGF concentrations were significantly higher in smokers compared with nonsmokers (0.45+/-0.03 vs. 0.35+/-0.02 ng/mL; P = 0.003). The serum HGF concentrations in patients with collaterals tended to be higher than those in patients without collaterals (0.43+/-0.03 vs. 0.35+/-0.02 ng/mL; P = 0.06). Moreover, in patients who underwent bypass surgery or angioplasty, serum HGF concentrations decreased from 0.41+/-0.03 to 0.21+/-0.04 ng/mL after treatment (P0.001). Serum HGF may be an useful marker for the diagnosis of PAOD. HGF may play an important role in angiogenesis and collateral vessel growth in PAOD.
- Published
- 1999
28. Effects of Propranolol on Cardiovascular and Neurohumoral Actions of Alcohol in Hypertensive Patients
- Author
-
Shuichi Takishita, Teruo Omae, Shunichi Kojima, Yuhei Kawano, and Hitoshi Abe
- Subjects
Adult ,Male ,medicine.medical_specialty ,Cardiac output ,Sympathetic Nervous System ,Alcohol Drinking ,Adrenergic beta-Antagonists ,Blood Pressure ,Vasodilation ,Propranolol ,Essential hypertension ,Plasma renin activity ,Norepinephrine ,Asian People ,Japan ,Heart Rate ,Internal medicine ,Renin ,Heart rate ,Internal Medicine ,Humans ,Medicine ,Cardiac Output ,Aged ,business.industry ,Hemodynamics ,General Medicine ,Middle Aged ,medicine.disease ,Blood pressure ,Endocrinology ,Echocardiography ,Hypertension ,Potassium ,Vascular Resistance ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug ,Blood sampling - Abstract
Alcohol ingestion acutely lowers blood pressure (BP) with vasodilation and sympathetic activation in Oriental subjects. We examined the effects of beta blockade on cardiovascular and neurohumoral actions of alcohol in Japanese men with mild-to-moderate essential hypertension. Ten hypertensive patients (54+/-5 years, mean+/-SE) were given 1 ml/kg of alcohol or isocaloric control drink with a light meal in the evening before and 5-7 days after treatment with propranolol (20 mg three times daily). BP and heart rate (HR) were measured every 30 min for 24 h in each period. Blood sampling and echocardiographic examination were carried out before (17.00 h) and after (19.00 h) intake of alcohol or control drink, Before treatment, alcohol ingestion caused significant decreases in BP, total peripheral resistance and serum potassium concentration, while it increased heart rate (HR), cardiac output (CO), plasma norepinephrine and plasma renin activity (PRA). Treatment with propranolol significantly decreased BP and HR for 24 h. Propranolol and alcohol showed an additive depressor effect on night-time BP, and the alcohol-induced hypotension was similar before and after propranolol treatment. The alcohol-induced changes in HR, CO, PRA and serum potassium were significantly attenuated by propranolol. These results suggest that activation of the sympathetic nervous system plays a role in alcohol-induced cardiac stimulation, renin release and hypokalemia through beta receptors. Moderate doses of beta-blockers may not modify alcohol-induced BP reduction in Oriental subjects with hypertension.
- Published
- 1999
29. Changes in Plasma Levels of Cyclic Nucleotides During Low-Density Lipoprotein Apheresis
- Author
-
Yuji Matsumoto, Toshihiko Sugi, Yuji Yoshitomi, Michiko Yano, Morio Kuramochi, and Shunichi Kojima
- Subjects
Male ,medicine.medical_specialty ,Hypercholesterolemia ,Bradykinin ,Nitric Oxide ,Guanidines ,Nitric oxide ,Adrenomedullin ,Cyclic nucleotide ,chemistry.chemical_compound ,Internal medicine ,Cyclic AMP ,medicine ,Humans ,Cyclic adenosine monophosphate ,Cyclic GMP ,Cyclic guanosine monophosphate ,Heparin ,Dextran Sulfate ,Anticoagulants ,General Medicine ,Middle Aged ,Benzamidines ,Lipoproteins, LDL ,Endocrinology ,Apheresis ,chemistry ,LDL apheresis ,Low-density lipoprotein ,Blood Component Removal ,Prostaglandins ,Nucleotides, Cyclic ,Peptides - Abstract
The negative charges of dextran sulfate (DS) used for low-density lipoprotein (LDL) apheresis activate the intrinsic coagulation pathway, accompanied by the production of bradykinin. This study was undertaken to see whether cyclic nucleotide plasma levels are affected by DS LDL apheresis. Previously, we showed the rise in plasma levels of prostaglandins and nitric oxide derivatives accompanied by the rise in bradykinin levels. The physiologic effects of prostaglandins and nitric oxide become manifest through the intracellular signal of cyclic nucleotides. The plasma levels of the cyclic nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) were examined when either of 2 anticoagulants, heparin or nafamostat mesilate (NM), was used during DS LDL apheresis. The plasma levels of cAMP during LDL apheresis using heparin were 9.2 +/- 0.3 (mean +/- SE) 12.4 +/- 0.6, 12.0 +/- 0.5, and 12.1 +/- 0.3 pmol/ml, respectively, at the 0, 1,000, 2,000, and 3,000 ml stages. The rise in cAMP levels was suppressed during apheresis using NM. There were no significant increases in cGMP during apheresis with heparin or with NM. There were significant negative correlations between changes in cAMP and those in the blood pressure. These findings suggest that bradykinin generated during apheresis exerts some physiologic effects via activation of the adenylate cyclase dependent pathway.
- Published
- 1998
30. Acute renal failure in accidental hypothermia of cold water immersion
- Author
-
Yuji Yoshitomi, Morio Kuramochi, Shunichi Kojima, and Makoto Ogi
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Ischemia ,Hypothermia ,Kidney ,urologic and male genital diseases ,Immersion ,Biopsy ,medicine ,Humans ,Acute tubular necrosis ,medicine.diagnostic_test ,business.industry ,Biopsy, Needle ,Acute Kidney Injury ,Kidney Tubular Necrosis, Acute ,medicine.disease ,Nephrology ,Accidents ,Renal biopsy ,medicine.symptom ,Endothelin receptor ,business ,Vasoconstriction ,Sports ,Kidney disease - Abstract
We report a 27-year-old Japanese man who developed acute renal failure associated with cold water immersion. The clinical course was consistent with that of acute renal failure attributable to acute tubular necrosis. A renal biopsy specimen showed patchy and focal loss of tubule cells, necrotic epithelium, interstitial edema, and arterial lumina obstructed by diffuse and severe intimal thickening. Endothelin increased more than five times in the early phase of the clinical course. Vasoconstriction and ischemia induced by cold exposure seem to lead to endothelin release. Endothelin may be related to the development of acute renal failure and intimal thickening.
- Published
- 1998
31. Plasma natriuretic peptides as indicators of left ventricular remodeling after myocardial infarction
- Author
-
Shunichi Kojima, Shuichi Takishita, Yuji Yoshitomi, Hisayuki Matsuo, Toshio Nishikimi, Morio Kuramochi, and Kenji Kangawa
- Subjects
Adult ,Male ,Cardiac Catheterization ,medicine.medical_specialty ,medicine.drug_class ,Myocardial Infarction ,Nerve Tissue Proteins ,Sudden death ,Atrial natriuretic peptide ,Internal medicine ,Natriuretic Peptide, Brain ,medicine ,Natriuretic peptide ,Humans ,cardiovascular diseases ,Myocardial infarction ,Ventricular remodeling ,Aged ,Aged, 80 and over ,business.industry ,Hemodynamics ,Middle Aged ,medicine.disease ,Brain natriuretic peptide ,Heart failure ,cardiovascular system ,Cardiology ,Female ,Hypertrophy, Left Ventricular ,Cardiology and Cardiovascular Medicine ,business ,Atrial Natriuretic Factor ,Biomarkers ,Blood sampling - Abstract
To investigate the relationship between natriuretic peptides and left ventricular remodeling after acute myocardial infarction, left ventriculography and blood sampling were performed on admission, after 1 month and after 3 months in 33 patients with acute myocardial infarction (15 anterior and 18 inferior). Plasma atrial and brain natriuretic peptide concentrations at 1 and 3 months were higher than those of controls (P
- Published
- 1998
32. Plasma Levels of Adrenomedullin in Patients with Acute Myocardial Infarction
- Author
-
Toshio Nishikimi, Shunichi Kojima, Morio Kuramochi, Hiroaki Matsuoka, Hisayuki Matsuo, Yuji Yoshitomi, Kenji Kangawa, Atsuro Miyata, and Shuichi Takishita
- Subjects
Adult ,Male ,medicine.medical_specialty ,Vasodilator Agents ,Myocardial Infarction ,Nerve Tissue Proteins ,Pulmonary Artery ,Adrenomedullin ,Atrial natriuretic peptide ,Internal medicine ,medicine.artery ,Natriuretic Peptide, Brain ,Blood plasma ,medicine ,Humans ,Heart Atria ,Myocardial infarction ,Aorta ,Aged ,Aged, 80 and over ,Heart Failure ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Brain natriuretic peptide ,Endocrinology ,Heart failure ,Pulmonary artery ,Cardiology ,Female ,Peptides ,business ,Atrial Natriuretic Factor ,hormones, hormone substitutes, and hormone antagonists - Abstract
1. Adrenomedullin, a newly identified vasorelaxant peptide, participates in the regulation of the cardiovascular system. To investigate the pathophysiological significance of adrenomedullin in patients with acute myocardial infarction, we measured plasma levels of adrenomedullin. 2. Cardiac catheterization was performed on admission, after 1 day, and after 4 weeks in 36 patients with acute myocardial infarction. We measured plasma levels of adrenomedullin, atrial natriuretic peptide and brain natriuretic peptide in the right atrium, pulmonary artery and aorta. 3. Plasma levels of adrenomedullin in the right atrium (mean ± SEM) were significantly increased on admission (4.2 ± 2.6 h) in patients with acute myocardial infarction (10.6 ± 1.0 pmol/l) compared with controls (5.2 ± 0.3 pmol/l, P < 0.01). In addition, plasma levels of adrenomedullin were further elevated in patients with congestive heart failure (12.3 ± 1.4 pmol/l) compared with patients without congestive heart failure (7.8 ± 0.6 pmol/l, P < 0.01). In patients with congestive heart failure, plasma adrenomedullin on admission significantly correlated with atrial natriuretic peptide and brain natriuretic peptide. 4. These results suggest that plasma adrenomedullin increases in the early phase of acute myocardial infarction and that volume expansion may be one of the additional stimuli for the release of adrenomedullin in patients with acute myocardial infarction complicated by congestive heart failure.
- Published
- 1998
33. Coronary vasoreactivity to ergonovine after angioplasty: difference between the infarct-related coronary artery and the noninfarct-related coronary artery
- Author
-
Shunichi Kojima, Morio Kuramochi, Michiko Yano, Yuji Matsumoto, Yuji Yoshitomi, and Toshihiko Sugia
- Subjects
Adult ,Male ,medicine.medical_specialty ,Vasodilator Agents ,medicine.medical_treatment ,Group ii ,Myocardial Infarction ,Coronary Vasospasm ,Isosorbide Dinitrate ,Coronary Angiography ,Balloon ,Angina Pectoris ,Angioplasty ,Internal medicine ,medicine ,Humans ,In patient ,cardiovascular diseases ,Myocardial infarction ,Angioplasty, Balloon, Coronary ,Ergonovine ,Aged ,Pharmacology ,Analysis of Variance ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Coronary Vessels ,Psychiatry and Mental health ,medicine.anatomical_structure ,Injections, Intravenous ,Cardiology ,Regression Analysis ,Female ,Myocardial infarction diagnosis ,business ,Cardiology and Cardiovascular Medicine ,Follow-Up Studies ,Artery - Abstract
BACKGROUND The vasoreactivity after direct percutaneous transluminal coronary angioplasty (PTCA) in patients with previous myocardial infarction remains unknown. We examined the constrictor response to ergonovine of the infarct-related coronary artery in comparison with that of noninfarct-related coronary artery after angioplasty. METHODS Ergonovine was administered intravenously to 17 patients with previous myocardial infarction (group I) and to 21 patients with stable angina (group II) 1 year after PTCA. The effects of ergonovine on lumen diameter were analysed quantitatively at the PTCA segment, nonPTCA segment (proximal to the PTCA segment), and nonPTCA artery. RESULTS The ergonovine-induced decrease in minimal lumen diameter at the PTCA segment was significant in group I (decrease from 2.12 +/- 0.56 to 1.39 +/- 0.74 mm, P < 0.01), but not in group II (decrease from 1.60 +/- 0.35 to 1.43 +/- 0.33 mm, NS). Patients in group I showed a constrictor response at the nonPTCA artery (decrease in diameter from 2.54 +/- 0.90 to 1.94 +/- 0.77 mm, P < 0.01), and a tendency to constrict at the nonPTCA segment (2.56 +/- 0.67 to 2.11 +/- 0.66 mm, P = 0.06), whereas those in group II showed no significant constrictor response to ergonovine at any of the three segments examined. The changes in diameter at the three segments in patients in group I were significantly greater than those in group II (all P < 0.01). Subtotal coronary spasm at the PTCA segment was provoked only in three patients in group I (18%). CONCLUSIONS The constrictor response to ergonovine of the infarct-related coronary artery was enhanced compared with that of the noninfarct-related coronary artery. This difference in coronary vasoreactivity at the angioplasty segment may be due to previous hypersensitivity of the smooth muscle.
- Published
- 1998
34. Echocardiography of a right atrial mass in hepatocellular carcinoma
- Author
-
Yutaka Ozeki, Yuji Matsumoto, Yuji Yoshitomi, Morio Kuramochi, Michiko Yano, Shunichi Kojima, and Toshihiko Sugi
- Subjects
Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Vena Cava, Inferior ,Intracardiac injection ,Metastasis ,Heart Neoplasms ,Fatal Outcome ,Internal medicine ,medicine ,Humans ,Neoplasm Invasiveness ,Heart Atria ,cardiovascular diseases ,Aged ,Ultrasonography ,business.industry ,Liver Neoplasms ,Antemortem Diagnosis ,Vascular surgery ,Neoplastic Cells, Circulating ,medicine.disease ,digestive system diseases ,Cardiac surgery ,Right atrial mass ,medicine.anatomical_structure ,Hepatocellular carcinoma ,cardiovascular system ,Cardiology ,Right atrium ,Female ,Radiology ,Cardiology and Cardiovascular Medicine ,business - Abstract
Antemortem diagnosis of hepatocellular carcinoma with a tumor thrombus extending into the right atrium has been rarely observed. Although echocardiography allows easy detection of the intracardiac mass, reports of echocardiographic descriptions of a right atrial mass associated with hepatocellular carcinoma are few. Herein, we describe two cases of hepatocellular carcinoma with a tumor thrombus in the right atrium detected by transthoracic echocardiography. In one of the patients we also performed transesophageal echocardiography. Neither patient had cardiac symptoms or signs. Thus, echocardiographic examination is very important in patients with hepatocellular carcinoma, with or without cardiac symptoms and signs.
- Published
- 1998
35. Changes In Plasma Levels of Nitric Oxide Derivative During Low-Density Lipoprotein Apheresis
- Author
-
Makoto Ogi, Toshihiko Sugi, Yuji Yoshitomi, Morio Kuramochi, Shunichi Kojima, and Yuji Matsumoto
- Subjects
Male ,Kininogen ,Dextran Sulfate ,Bradykinin ,Plasmapheresis ,General Medicine ,Kallikrein ,Middle Aged ,Pharmacology ,Nitric Oxide ,Nitric oxide ,Lipoproteins, LDL ,chemistry.chemical_compound ,Apheresis ,chemistry ,Biochemistry ,LDL apheresis ,Low-density lipoprotein ,Humans ,Female ,Kallikreins ,Aged ,Lipoprotein - Abstract
The negative charges of dextran-sulfate (DS) used for low-density lipoprotein (LDL) apheresis activates the intrinsic coagulation pathway, in which plasma kallikrein acts on high-molecular-weight kininogen (HMWK) to produce large amounts of bradykinin. This study was undertaken to see whether bradykinin generated during DS LDL apheresis has some physiologic effects in vivo. The plasma levels of bradykinin and nitric oxide derivatives (NOx) were examined when either of 2 anticoagulants, heparin or nafamostat mesilate (NM), was used during DS LDL apheresis. Although anticoagulative action by NM depends on the inhibition of thrombin activity, this substance also inhibits the activity of plasma kallikrein. During apheresis using heparin, the marked increase in bradykinin levels (before apheresis, 18 +/- 3 (mean +/- SE, n = 5) pg/ml; after apheresis 470 +/- 140, p < 0.01) was associated with the increase in NOx (before apheresis 50 +/- 11 pg/ml; after apheresis 66 +/- 15). Interestingly, these changes in bradykinin and NOx levels were suppressed during apheresis using NM. The changes in plasma NOx levels were negatively correlated with those in blood pressures. These findings suggest that bradykinin generated during apheresis exerts some physiologic effects by means of activation of endothelium-derived relaxant factor (EDRF). Our results support the view that bradykinin produced during DS LDL apheresis has physiologic significance.
- Published
- 1997
36. Apheresis Technology for Prevention and Regression of Atherosclerosis: An Overview
- Author
-
Shunichi Kojima, Motoo Tsushima, Akito Kawaguchi, Mariko Harada-Shiba, and Akira Yamamoto
- Subjects
medicine.medical_specialty ,Arteriosclerosis ,Plasma Substitutes ,Familial hypercholesterolemia ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,Myocardial infarction ,Cellulose ,Coronary atherosclerosis ,Arteriosclerosis obliterans ,business.industry ,Dextran Sulfate ,General Medicine ,medicine.disease ,Lipoproteins, LDL ,Treatment Outcome ,Apheresis ,chemistry ,LDL apheresis ,Low-density lipoprotein ,Immunology ,Blood Component Removal ,lipids (amino acids, peptides, and proteins) ,business ,Follow-Up Studies ,Lipoprotein - Abstract
Low density lipoprotein (LDL) apheresis is at present one method of treatment in homozygous cases of familial hypercholesterolemia (FH). It is also effective in the prevention of the development of coronary atherosclerosis in patients with heterozygous FH and other types of mild hypercholesterolemia, leading to the regression of the stenosing lesions. In this paper, an overview is presented on the development of the devices for LDL apheresis and its short-and long-term effects on FH mainly based upon experience with the Liposorber system. LDL apheresis has served to protect the lives of patients from life threatening diseases like myocardial infarction although observations for more than 10 years in some laboratories have shown that the progression of atherosclerosis has taken place in many patients, and more importantly, the involvement of the aortic valve with calcification has developed. especially in patients who had homozygous FH, making this the most obstinate complication of FH. Therefore, more aggressive treatment or the combination of LDL apheresis with other therapies is required in the future. LDL apheresis has also been approved for the treatment of glomerulosclerosis and arteriosclerosis obliterans.
- Published
- 1997
37. Effects of occlusal hypofunction and its recovery on PDL structure and expression of VEGF and bFGF in rats
- Author
-
Shunichi Kojima, Masahide Motokawa, Masato Kaku, Yayoi Matsuda, Kotaro Tanimoto, A. Kimura, and Chikako Sumi
- Subjects
Male ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,biology ,Chemistry ,Periodontal Ligament ,VEGF receptors ,Basic fibroblast growth factor ,Anatomy ,Rats ,Vascular endothelial growth factor ,chemistry.chemical_compound ,Endocrinology ,stomatognathic system ,Internal medicine ,medicine ,biology.protein ,Periodontal fiber ,Animals ,Fibroblast Growth Factor 2 ,Rats, Wistar ,General Dentistry ,Dental alveolus ,Malocclusion - Abstract
The purpose of this study was to clarify whether occlusal hypofunction and its recovery affect the structure of the periodontal ligament (PDL) and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in rats. Forty-eight Wistar rats aged 5 weeks were used and randomly divided into three groups: the hypofunctional group (HG), recovery group (RG), and control group (CG). In HG and RG, appliances were attached to the maxillary and mandibular incisors. In HG, appliances were set for 11 weeks. In RG, appliances were set for 7 weeks. Appliances were then removed at 0, 1, 3, 7, 14, and 28 days. Untreated rats served as CG. Histological sections were prepared and immunohistochemically stained for VEGF and bFGF. Three groups were evaluated for PDL area and the number of VEGF and bFGF immunopositive cells in PDL. The number of immunopositive cells and PDL area in CG and RG were significantly larger when compared with HG, and PDL area in RG was similar to that in CG. In the recovery process, PDL area and number of VEGF-positive cells in PDL increased from days 0 to 7 and decreased from days 7 to 28. Conversely, the number of bFGF-positive cells in PDL increased significantly after day 1 and peaked at 28 days. This study suggests that occlusal stimuli regulate PDL area through expression of VEGF and bFGF in rat PDL. Occlusal stimuli are able to regulate the expression of VEGF and bFGF in PDL cells, and these growth factors may lead to alveolar bone remodeling in PDL.
- Published
- 2013
38. Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field
- Author
-
Tahsin Raquib Abonti, Toshitsugu Kawata, Masato Kaku, Kazuo Tanne, Hanaka Shikata, Shunichi Kojima, Shotoku Kojima, Hiromi Sumi, Masahide Motokawa, and Tadashi Fujita
- Subjects
Male ,Cell Survival ,Biology ,Regenerative medicine ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,chemistry.chemical_compound ,Cryoprotective Agents ,medicine ,Oil Red O ,Animals ,Dimethyl Sulfoxide ,Cells, Cultured ,Cell Proliferation ,Dimethyl sulfoxide ,Regeneration (biology) ,Mesenchymal stem cell ,Ice ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,Rats, Inbred F344 ,Rats ,medicine.anatomical_structure ,Magnetic Fields ,chemistry ,Immunology ,Alkaline phosphatase ,Bone marrow ,General Agricultural and Biological Sciences ,human activities - Abstract
Mesenchymal stem cells (MSCs) can be used for the regeneration of various tissues and cryopreservation of MSCs is so important for regenerative medicine. The purpose of this study was to evaluate the influences of cryopreservation on MSCs by use of a programmed freezer with a magnetic field (CAS freezer). MSCs were isolated from bone marrow of rat femora. The cells were frozen by a CAS freezer with 10% dimethyl sulfoxide (Me2SO) and cryopreserved for 7 days at a temperature of −150 °C. Immediately after thawing, the number of survived cells was counted. The cell proliferation also examined after 48 h culture. Next, MSCs were frozen by two different freezers; CAS freezer and a conventional programmed freezer without magnetic field. Then, osteogenic and adipogenic differentiations of cryopreserved cells were examined. As a result, survival and proliferation rates of MSCs were significantly higher in CAS freezer than in the non-magnetic freezer. Alizarin positive reaction, large amount of calcium quantification, and greater alkaline phosphatase activity were shown in both the non-cryopreserved and CAS groups after osteogenic differentiation. Moreover, Oil Red O staining positive reaction and high amount of PPARγ and FABP4 mRNAs were shown in both the non-cryopreserved and CAS groups after adipogenic differentiation. From these findings, it is shown that a CAS freezer can maintain high survival and proliferation rates of MSCs and maintain both adipogenic and osteogenic differentiation abilities. It is thus concluded that CAS freezer is available for cryopreservation of MSCs, which can be applied to various tissue regeneration.
- Published
- 2013
39. Effect of Nafamostat Mesilate on Bradykinin Generation and Hemodynamics During LDL Apheresis
- Author
-
Shunichi Kojima, Akira Yamamoto, Mariko Shiba, and Morio Kuramochi
- Subjects
Adult ,Male ,Adolescent ,High-molecular-weight kininogen ,medicine.drug_class ,Hypercholesterolemia ,Biomedical Engineering ,Medicine (miscellaneous) ,Bradykinin ,Bioengineering ,Pharmacology ,Guanidines ,Biomaterials ,chemistry.chemical_compound ,medicine ,Humans ,Factor XII ,Heparin ,Anticoagulant ,Hemodynamics ,Prekallikrein ,General Medicine ,Middle Aged ,Benzamidines ,Lipoproteins, LDL ,chemistry ,LDL apheresis ,Immunology ,Blood Component Removal ,Female ,medicine.drug ,Lipoprotein - Abstract
Dextran-sulfate (DS) cellulose used for low-density lipoprotein (LDL) apheresis seems to be a weak activator of the contact phase of the intrinsic coagulation pathway because the surface of this substance has negative charges. Heparin, a commonly used anticoagulant, has no effect on this process whereas the process is inhibited by a newly developed anticoagulant, nafamostat mesilate (NM). The effects on bradykinin generation were compared between heparin and NM. Five patients with severe hypercholesterolemia were treated with LDL apheresis using either heparin or NM on a different day. During apheresis with heparin, factor XII, high molecular weight kininogen, and prekallikrein were markedly decreased by passing through the DS column. A distinct generation of bradykinin was observed by passing plasma through the DS column, and this led to the rise of bradykinin levels from 12 +/- 5 (mean +/- SE) to 72 +/- 14 pg/ml after treatment of 1,000 ml of plasma. NM suppressed almost completely the rise of bradykinin levels. Although blood pressure was apt to decrease during apheresis with heparin, there was no significant difference in blood pressure between heparin and NM. Since an angiotensin-converting enzyme inhibitor may lead to a marked rise in blood levels of bradykinin by suppressing its degradation, the use of NM is recommended for apheresis in patients taking this drug.
- Published
- 1995
40. Initial Flight Operations of the Miniature Propulsion System Installed on Small Space Probe: PROCYON
- Author
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Ryu Funase, Jun Asakawa, Kimiya Komurasaki, Shunichi Kojima, Toshihiro Matsuguma, Hiroyuki Koizumi, Junichi Nakatsuka, Yuichi Nakagawa, Hiroki Kawahara, Kazuya Yaginuma, and Yusuke Nakamura
- Subjects
010302 applied physics ,Physics ,Ion thruster ,business.industry ,Flight operations ,NASA Deep Space Network ,Propulsion ,01 natural sciences ,Small Satellite ,010305 fluids & plasmas ,Deep Space ,Microthruster ,0103 physical sciences ,Space probe ,Aerospace engineering ,Ion Thruster ,business - Abstract
著者人数: 11名, 資料番号: SA1160200000
- Published
- 2016
41. Total Impulse Increase of a Micro-Solid Rocket Using a Stack of B/KNO3 Pellets
- Author
-
Jun Asakawa, Kimiya Komurasaki, Shunichi Kojima, Hiroyuki Koizumi, Nobu Okada, and Masakatsu Nakano
- Subjects
020301 aerospace & aeronautics ,Materials science ,Monopropellant rocket ,business.industry ,Pellets ,Rocket propellant ,02 engineering and technology ,Impulse (physics) ,01 natural sciences ,0203 mechanical engineering ,0103 physical sciences ,Aerospace engineering ,Solid-fuel rocket ,business ,010303 astronomy & astrophysics - Published
- 2016
42. Primary Cardiac Lymphoma
- Author
-
Shunichi Kojima, Masao Saotome, Yuji Yoshitomi, and Morio Kuramochi
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Lymphoma ,Cyclophosphamide ,Heart disease ,Mediastinal lymphadenopathy ,medicine.medical_treatment ,Autopsy ,030204 cardiovascular system & hematology ,Pericardial effusion ,Diagnosis, Differential ,Heart Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,Prednisone ,medicine ,Humans ,030212 general & internal medicine ,Aged ,Chemotherapy ,Lung ,business.industry ,medicine.disease ,medicine.anatomical_structure ,Postmortem Changes ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Primary cardiac lymphoma, which is very rare, is generally regarded to have a poor prognosis. A case of a 69-year-old man with primary cardiac lymphoma diagnosed by antemortem exam ination is reported. A computed tomography scan of the chest demonstrated a huge right atrial mass with invasion into the other chambers. No mediastinal lymphadenopathy was detected. Cytologic analysis of pericardial effusion revealed diffuse large B-cell type non- Hodgkin malignant lymphoma. The patient died on the 18th day of chemotherapy (cyclophos phamide, hydroxydaunomycin, oncovin, and prednisone) due to low-output syndrome and multiple organ failure. At autopsy, massive gray-white tumor almost occupied the right atrium and invaded the right inferior lobe of the lung. Although prognosis of primary cardiac lymphoma remains poor, early diagnosis may improve the prognosis.
- Published
- 2002
43. Efficacy of percutaneous transluminal coronary angioplasty in chronic hemodialysis patients: our second report
- Author
-
Hiroaki Matsuoka, Toru Sanai, Masahito Imanishi, Takanori Yasu, Yuhei Kawano, Teruo Omae, Shunichi Kojima, Yoshitsugu Kitoh, Kazuo Haze, Hiroshi Nonogi, Genjiro Kimura, and Shunichi Miyazaki
- Subjects
Percutaneous transluminal coronary angioplasty ,medicine.medical_specialty ,business.industry ,Internal medicine ,Cardiology ,Medicine ,Chronic hemodialysis ,business - Abstract
薬物治療に抵抗する狭心症を合併した維持血液透析中の12例 (男8, 女4例, 平均年齢は56.3歳) に対する経皮的冠動脈形成術 (PTCA) の治療成績について検討した. 合計27狭窄病変に対してPTCAを施行し, 22狭窄病変 (82%) の拡張に成功した. 不成功例は2例で, そのうちの1例は小脳出血により死亡したが, 他の11例では重篤な合併症は出現しなかった. 患者成功は12例中10例 (83%) で, 非透析例と同等の治療成績であった. 11例の平均追跡期間は2年5か月で, 狭心症が4例に再発し, 3例には再度PTCAを施行し, 全例成功した. 透析患者に対する冠動脈バイパス術はいまだにhigh riskとされていることから, 透析患者の難治性狭心症に対しては, 解剖学的に禁忌・不適応でない限り, PTCAが第一選択と考えられた.
- Published
- 1993
44. In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field
- Author
-
Junji Ohtani, Hiroyuki Koseki, Toshitsugu Kawata, Kazuo Tanne, Tadashi Fujita, Akemi Sumi, Sara Abedini, Masato Kaku, Masahide Motokawa, Shunichi Kojima, Norio Ohwada, and Hiroko Kamada
- Subjects
Male ,Adolescent ,Cell Survival ,Periodontal Ligament ,Root Resorption ,Dentistry ,Gene Expression ,Orthodontics ,Real-Time Polymerase Chain Reaction ,Cryopreservation ,Collagen Type I ,Andrology ,chemistry.chemical_compound ,In vivo ,Gene expression ,Periodontal fiber ,Animals ,Humans ,RNA, Messenger ,Cells, Cultured ,Dimethyl sulfoxide ,business.industry ,Organ Preservation ,Alkaline Phosphatase ,Rats ,Transplantation ,Real-time polymerase chain reaction ,Magnetic Fields ,chemistry ,Alkaline phosphatase ,Tooth Replantation ,business - Abstract
Introduction The purpose of this study was to examine the effect of a new cryopreservation method with a magnetic field on periodontal regeneration in vitro and in vivo. Methods Human periodontal ligament cells were frozen in 10% dimethyl sulfoxide by using a programmed freezer with a magnetic field. Cells were cryopreserved for 3 days at −150°C. Immediately after thawing, collagen type I and alkaline phosphatase gene expression were determined by real-time polymerase chain reaction. Incisors were extracted from 15-week-old Wistar rats and cryopreserved or dried for 3 days. Then the incisors were replanted into the same sockets. Ninety days after transplantation, they were observed under light microscopy. Results There was no difference in the messenger RNA expression of collagen type I between the cryopreserved and the control groups. The expression of alkaline phosphatase messenger RNA in the cryopreserved group was slightly decreased compared with the control group. There was no progressive root resorption in the teeth that were replanted immediately (control group) or cryopreserved. However, there was widespread root resorption and ankylosis in the dried teeth. Conclusions These results show that a magnetic field programmed freezer can be successfully used for cryopreservation of teeth.
- Published
- 2010
45. Effects of cryopreservation with a newly-developed magnetic field programmed freezer on periodontal ligament cells and pulp tissues
- Author
-
N. Ohwada, Hiroyuki Koseki, Kazuo Tanne, Hiromi Sumi, Junji Ohtani, Sara Abedini, Shunichi Kojima, Tsuyoshi Fujita, Masahide Motokawa, Masato Kaku, and Toshitsugu Kawata
- Subjects
Molar ,Vascular Endothelial Growth Factor A ,Cryobiology ,Cell Survival ,Periodontal Ligament ,Cell Culture Techniques ,Dentistry ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,chemistry.chemical_compound ,Magnetics ,Electromagnetic Fields ,stomatognathic system ,Nerve Growth Factor ,Periodontal fiber ,Humans ,Regeneration ,Tooth Root ,Dental Pulp ,business.industry ,General Medicine ,Equipment Design ,Alkaline Phosphatase ,Vascular endothelial growth factor ,stomatognathic diseases ,Vascular endothelial growth factor A ,Nerve growth factor ,chemistry ,Pulp (tooth) ,General Agricultural and Biological Sciences ,business ,Tooth - Abstract
The purpose of this study was to evaluate the effects of long-term cryopreservation on the isolated human periodontal ligament cells (PDL) and pulp tissues. In the first part of study, 10 freshly extracted teeth were selected and divided into two groups. In the cryopreserved group, the teeth were frozen for 5 years using a programmed freezer combined with a magnetic field, known as Cells Alive System "CAS". As for the control group, freshly extracted teeth were used. In each group, extracted PDL tissues were cultured and gene expression and protein concentration of collagen type I, alkaline-phosphatase (ALP) and vascular endothelial growth factor (VEGF) was compared between the two groups. In the second part, pulp tissues were obtained from 10 mature and immature third molars which were freshly extracted or cryopreserved for three months. Expression of VEGF and nerve growth factor (NGF) mRNAs and the protein concentration in the supernatant were investigated. Results indicated that long-term cryopreservation with the use of CAS freezer cannot affect the growth rate and characteristics of PDL cells. There was no significant difference in VEGF expression and VEGF and NGF protein concentration of pulp cells derived from cryopreserved teeth with immature apex and control group with mature root formation. Finally, proper PDL regeneration and appropriate apexogenesis after transplanting magnetically cryopreserved immature tooth was clinically confirmed. These findings demonstrate that teeth banking with the use of magnetic field programmed freezer can be available for future autotransplantation as a treatment modality for replacing missing teeth.
- Published
- 2010
46. SODIUM AND NORADRENALINE IN CEREBROSPINAL FLUID AND BLOOD IN SALT-SENSITIVE AND NON-SALT-SENSITIVE ESSENTIAL HYPERTENSION
- Author
-
Teruo Omae, Morio Kuramochi, Minoru Kawamura, Hitoshi Abe, Genjiro Kimura, Shunichi Kojima, Hiroki Yoshimi, Masahito Imanishi, Terunao Ashida, Kaoru Yoshida, and Yuhei Kawano
- Subjects
Adult ,Male ,medicine.medical_specialty ,Physiology ,Sodium ,chemistry.chemical_element ,Blood Pressure ,Essential hypertension ,Plasma renin activity ,Norepinephrine ,Cerebrospinal fluid ,Heart Rate ,Physiology (medical) ,Internal medicine ,Renin ,Renin–angiotensin system ,medicine ,Humans ,Salt intake ,Aged ,Pharmacology ,Sodium, Dietary ,Middle Aged ,medicine.disease ,Endocrinology ,Blood pressure ,chemistry ,Hypertension ,Female ,Blood sampling - Abstract
1. The effects of dietary sodium on blood pressure and levels of sodium, other electrolytes and noradrenaline (NA) in the cerebrospinal fluid (CSF) and blood of 15 patients with essential hypertension were studied. The CSF and blood sampling was carried out after 7 days of a high salt intake (16-18 g/day) and after 7 days of a low salt intake (1-3 g/day). 2. Blood pressure and sodium concentrations in CSF and serum were significantly higher in the high salt period than the low salt period (CSF Na+ concentration: 147.7 +/- 0.4 mmol/L vs 145.3 +/- 0.5 mmol/L; P less than 0.001). Levels of CSF pressure and potassium or calcium concentrations were not different between the two periods. Plasma NA and plasma renin activity (PRA) were lower and CSF NA levels tended to be lower in the high salt period. 3. The levels and the changes in sodium and NA in CSF were not significantly different between the salt-sensitive (n = 8) and the non-salt-sensitive (n = 7) subjects, but the changes in plasma NA and PRA were smaller in the salt-sensitive subjects. 4. These results indicate that the sympathetic nervous system is less suppressed in salt-sensitive subjects during high salt intake. This may be due to altered neural responsiveness to sodium loading rather than being greater increases in sodium concentration in the central nervous system.
- Published
- 1992
47. Beneficial effect of stopping smoking on future cardiac events in male smokers with previous myocardial infarction
- Author
-
Shunichi Kojima, Kohichi Okita, Hisakazu Yasuda, Naoya Matsumura, Isao Sato, Hirotaka Nishijima, and Masayuki Nishida
- Subjects
Adult ,Male ,medicine.medical_specialty ,Multivariate analysis ,Physiology ,medicine.medical_treatment ,Myocardial Infarction ,Coronary Angiography ,Group B ,Japan ,Risk Factors ,Internal medicine ,medicine ,Humans ,Obesity ,Myocardial infarction ,Risk factor ,Univariate analysis ,Chi-Square Distribution ,business.industry ,Middle Aged ,medicine.disease ,Surgery ,Relative risk ,Hypertension ,Exercise Test ,Smoking cessation ,Smoking Cessation ,Cardiology and Cardiovascular Medicine ,business ,Chi-squared distribution ,Follow-Up Studies - Abstract
We assessed the effect of stopping smoking on future cardiac events (cardiac death and non-fatal myocardial infarction (MI)) in 90 male smokers with previous MI by a prospective follow-up study. At the time of enrollment, the patients were divided into 2 groups according to their current smoking status (Group A: those who had stopped smoking (n = 60), and Group B: those who were still smoking (n = 30)). Follow-up was performed prospectively by annual postal questionnaires. During the mean follow-up period of 3.1 +/- 1.4 years, 13 cardiac events (11 cardiac deaths and 2 cases of non-fatal MI) occurred in the 87 evaluable patients. The cardiac event rates in Groups A and B were 8.5% and 28.6%, respectively, and the relative risk was 3.4 (Group A vs B, p less than 0.05) by univariate analysis. The relative risk using multivariate analysis (Cox) was also statistically significant (3.1, p less than 0.05). HDL-cholesterol was significantly lower in Group B than in Group A, but other coronary risk factors apart from smoking were not significantly different between the two groups. At the end of the follow-up period, 89.8% of the patients in Group A remained nonsmokers and 21% of the patients in Group B had stopped smoking.Stopping smoking significantly reduced the risk of future cardiac events in male smokers with previous MI. This prospective follow-up study demonstrated that a significant reduction in the risk of future cardiac events could be achieved by stopping smoking in this group of patients.
- Published
- 1992
48. Effect of a Hypocaloric Diet on Blood Pressure and Sodium Balance in Overweight Patients with Essential Hypertension
- Author
-
Minoru Kawamura, Takayuki Akasaka, Keiichi Ito, Shunichi Kojima, Tamako Kasatsuki, Katsuhiko Hiramori, and Teruo Omae
- Subjects
medicine.medical_specialty ,Mean arterial pressure ,Physiology ,business.industry ,Sodium ,chemistry.chemical_element ,Overweight ,Essential hypertension ,medicine.disease ,Natriuresis ,Excretion ,Endocrinology ,Blood pressure ,chemistry ,Weight loss ,Internal medicine ,Internal Medicine ,medicine ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Abstract
This study was performed to elucidate the effect of a hypocaloric diet on blood pressure and sodium balance in overweight women with essential hypertension. Hospitalized patients were fed a hypocaloric diet (900 kcal/day) for 3 weeks after being fed a standard diet (SD) for more than 2 weeks. During the study, the sodium and potassium intake was kept constant as was the level of exercise activity. After the mean arterial pressure (MAP) and body weight had stabilized during the last week on the SD, caloric restriction significantly reduced the body weight by 1.5kg in the first week and by 0.8-0.9 kg in subsequent weeks, and reduced the MAP by 5mmHg and by 1-3mmHg, respectively. Although caloric restriction reduced the excretion of urinary norepinephrine and C-peptide, and elevated the serum levels of ketone bodies that would induce natriuresis, the urinary sodium excretion remained unchanged, indicating that sodium balance was unchanged even by caloric restriction. These results suggest that caloric restriction lowers the blood pressure in a short time with no significant change in sodium balance. (Hypertens Res 1992; 15: 137-142)
- Published
- 1992
49. Different mechanisms of polyuria and natriuresis associated with paroxysmal supraventricular tachycardia
- Author
-
Tohru Ohe, Teruo Omae, Takashi Fujii, Masahito Imanishi, and Shunichi Kojima
- Subjects
Adult ,Male ,Tachycardia ,medicine.medical_specialty ,Vasopressins ,Natriuresis ,Blood Pressure ,Urine ,Kidney ,Dinoprostone ,Excretion ,Polyuria ,Heart Rate ,Internal medicine ,Tachycardia, Supraventricular ,medicine ,Humans ,Prostaglandin E2 ,Tachycardia, Paroxysmal ,business.industry ,Osmolar Concentration ,Middle Aged ,Endocrinology ,Renal physiology ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Atrial Natriuretic Factor ,Antidiuretic ,medicine.drug - Abstract
The mechanism of polyuria associated with paroxysmal supraventricular tachycardia (SVT) was investigated in 8 patients. SVT was induced artificially and sustained for 60 minutes. Urine and blood samples were collected every 30 minutes. During the latter half of SVT, urine flow increased twofold in the control subjects before SVT. Urinary sodium excretion increased significantly (p less than 0.01) within 30 minutes after SVT. Urinary excretion of antidiuretic hormone (ADH) decreased (p less than 0.01) during the latter half of SVT and increased (p less than 0.01) after SVT, respectively. Plasma level of ADH did not change during SVT but increased (p less than 0.05) after SVT. The concentration of plasma atrial natriuretic polypeptide (ANP) increased significantly (p less than 0.05) before SVT ended. Urinary excretion of prostaglandin E2 increased significantly (p less than 0.05) after termination of SVT. The percent changes in the urinary excretion of prostaglandin E2 were correlated (r = 0.713, p less than 0.001) with those of ADH. There was also a correlation (r = 0.6, p less than 0.001) between the percent changes in the urinary excretion of prostaglandin E2 and those of sodium. Their findings suggest that the polyuria during SVT is attributed mainly to the inhibition of ADH release and that the natriuresis after SVT is due not only to the increased ANP but also to the increased renal prostaglandin E2 probably stimulated by ADH.
- Published
- 1991
50. Role of systolic blood pressure in the prognosis of hemodialyzed patients
- Author
-
Morio Kuramochi, Hitoshi Abe, Genjiro Kimura, Shunroku Baba, Teruo Omae, Shunichi Kojima, Toru Sanai, Hiroki Yoshimi, Jun Tomita, Minoru Kawamura, Masahito Imanishi, Terunao Ashida, Kaoru Yoshida, and Yuhei Kawano
- Subjects
medicine.medical_specialty ,Blood pressure ,business.industry ,Internal medicine ,medicine.medical_treatment ,Mortality rate ,medicine ,Cardiology ,business ,Dialysis - Abstract
近年, 透析患者の循環器疾患による死亡率は年々増加してきている. そこで, 透析患者の予後を決定する上で, 血圧がどの程度関与しているか検討した. 1977年から1987年までに当施設で血液透析に導入し, 転院し得た慢性腎不全患者195例 (男123例, 女72例, 平均年齢54±1歳) について導入期および, 維持期 (転院時) における血圧と1990年1月現在における生死との関連を検討した. 3年以上生存した生存群132例と3年未満に死亡した死亡群46例との比較では, 死亡群で平均年齢が高く (61±2歳vs 50±1歳), 糖尿病性腎症の割合が高く, 収縮期血圧は導入期 (178±4mmHg vs 167±2mmHg), 維持期 (165±4mmHg vs 147±2mmHg) とも高値であった. ただし, 拡張期血圧に関しては導入期, 維持期とも両群間に有意差を認めなかった. 非糖尿病性腎症例に限ると維持期収縮期血圧のみ死亡群で有意に高値であった. そこで, 維持期収縮期血圧に着目し160mmHg以上をHT群, 160mmHg未満をNT群とし, 累積生存率を比較検討した. 195例全例でも, 非糖尿病性腎症でも, 糖尿病性腎症例でもHT群に比しNT群で有意に生存率が高値を示した. 導入期から維持期に至る収縮期血圧の推移別に, 1. 導入期収縮期血圧が160mmHg以上であったが, 維持期には160mmHg未満に低下したHT-NT群, 2. 維持期にもなお160mmHg以上を示したHT-HT群, および3. 導入期, 維持期を通して160mmHg未満であったNT-NT群の3群に分類し累積生存率を比較した. HT-NT群はHT-HT群に比し有意に生存率が高くNT-NT群と有意差のないレベルに達していた. 以上より, 透析導入によって是正された後の維持期収縮期血圧が透析患者の予後を決定する重要な因子と考えられた.
- Published
- 1991
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