1. Cloning of two new cry genes from Bacillus thuringiensis subsp. wuhanensis strain
- Author
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Shui-Shang Kao, Ching-Chou Tzeng, Kin-Fu Chak, Jong-Huon Lin, and White-Shang Kuo
- Subjects
clone (Java method) ,Bacterial Toxins ,Molecular Sequence Data ,Bacillus thuringiensis ,Sequence alignment ,Molecular cloning ,Moths ,Applied Microbiology and Biotechnology ,Microbiology ,Polymerase Chain Reaction ,Lethal Dose 50 ,Bacterial Proteins ,Botany ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Peptide sequence ,Toxins, Biological ,Cloning ,Genetics ,Bacillaceae ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,fungi ,Plutella ,General Medicine ,biology.organism_classification ,Genes, Bacterial ,Larva ,Butterflies ,Sequence Alignment - Abstract
With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8–78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.
- Published
- 2000