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Cloning of two new cry genes from Bacillus thuringiensis subsp. wuhanensis strain
- Source :
- Current microbiology. 40(4)
- Publication Year :
- 2000
-
Abstract
- With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8–78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.
- Subjects :
- clone (Java method)
Bacterial Toxins
Molecular Sequence Data
Bacillus thuringiensis
Sequence alignment
Molecular cloning
Moths
Applied Microbiology and Biotechnology
Microbiology
Polymerase Chain Reaction
Lethal Dose 50
Bacterial Proteins
Botany
Animals
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Toxins, Biological
Cloning
Genetics
Bacillaceae
biology
Base Sequence
Sequence Homology, Amino Acid
fungi
Plutella
General Medicine
biology.organism_classification
Genes, Bacterial
Larva
Butterflies
Sequence Alignment
Subjects
Details
- ISSN :
- 03438651
- Volume :
- 40
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Current microbiology
- Accession number :
- edsair.doi.dedup.....c63669926e59e07f43b71702c568f006