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25 results on '"Shlapakova, Iryna N."'

7. HCN2/SkM1 Gene Transfer Into Canine Left Bundle Branch Induces Stable, Autonomically Responsive Biological Pacing at Physiological Heart Rates

Author

Boink, Gerard J.J., primary, Duan, Lian, additional, Nearing, Bruce D., additional, Shlapakova, Iryna N., additional, Sosunov, Eugene A., additional, Anyukhovsky, Evgeny P., additional, Bobkov, Eugene, additional, Kryukova, Yelena, additional, Ozgen, Nazira, additional, Danilo, Peter, additional, Cohen, Ira S., additional, Verrier, Richard L., additional, Robinson, Richard B., additional, and Rosen, Michael R., additional

Published
2013
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8. Effect of Skeletal Muscle Na + Channel Delivered Via a Cell Platform on Cardiac Conduction and Arrhythmia Induction

Author

Boink, Gerard J.J., primary, Lu, Jia, additional, Driessen, Helen E., additional, Duan, Lian, additional, Sosunov, Eugene A., additional, Anyukhovsky, Evgeny P., additional, Shlapakova, Iryna N., additional, Lau, David H., additional, Rosen, Tove S., additional, Danilo, Peter, additional, Jia, Zhiheng, additional, Ozgen, Nazira, additional, Bobkov, Yevgeniy, additional, Guo, Yuanjian, additional, Brink, Peter R., additional, Kryukova, Yelena, additional, Robinson, Richard B., additional, Entcheva, Emilia, additional, Cohen, Ira S., additional, and Rosen, Michael R., additional

Published
2012
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9. Ca 2+ -Stimulated Adenylyl Cyclase AC1 Generates Efficient Biological Pacing as Single Gene Therapy and in Combination With HCN2

Author

Boink, Gerard J.J., primary, Nearing, Bruce D., additional, Shlapakova, Iryna N., additional, Duan, Lian, additional, Kryukova, Yelena, additional, Bobkov, Yevgeniy, additional, Tan, Hanno L., additional, Cohen, Ira S., additional, Danilo, Peter, additional, Robinson, Richard B., additional, Verrier, Richard L., additional, and Rosen, Michael R., additional

Published
2012
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13. Epicardial Border Zone Overexpression of Skeletal Muscle Sodium Channel SkM1 Normalizes Activation, Preserves Conduction, and Suppresses Ventricular Arrhythmia

Author

Lau, David H., primary, Clausen, Chris, additional, Sosunov, Eugene A., additional, Shlapakova, Iryna N., additional, Anyukhovsky, Evgeny P., additional, Danilo, Peter, additional, Rosen, Tove S., additional, Kelly, Caitlin, additional, Duffy, Heather S., additional, Szabolcs, Matthias J., additional, Chen, Ming, additional, Robinson, Richard B., additional, Lu, Jia, additional, Kumari, Sinhu, additional, Cohen, Ira S., additional, and Rosen, Michael R., additional

Published
2009
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16. Repolarization Gradients in the Canine Left Ventricle Before and After Induction of Short-Term Cardiac Memory

Author

Janse, Michiel J., primary, Sosunov, Eugene A., additional, Coronel, Ruben, additional, Opthof, Tobias, additional, Anyukhovsky, Evgeny P., additional, de Bakker, Jacques M.T., additional, Plotnikov, Alexei N., additional, Shlapakova, Iryna N., additional, Danilo, Peter, additional, Tijssen, Jan G.P., additional, and Rosen, Michael R., additional

Published
2005
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18. Human Mesenchymal Stem Cells as a Gene Delivery System to Create Cardiac Pacemakers

Author

Potapova, Irina, primary, Plotnikov, Alexei, additional, Lu, Zhongju, additional, Danilo, Peter, additional, Valiunas, Virginijus, additional, Qu, Jihong, additional, Doronin, Sergey, additional, Zuckerman, Joan, additional, Shlapakova, Iryna N., additional, Gao, Junyuan, additional, Pan, Zongming, additional, Herron, Alan J., additional, Robinson, Richard B., additional, Brink, Peter R., additional, Rosen, Michael R., additional, and Cohen, Ira S., additional

Published
2004
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19. Biological Pacemaker Implanted in Canine Left Bundle Branch Provides Ventricular Escape Rhythms That Have Physiologically Acceptable Rates

Author

Plotnikov, Alexei N., primary, Sosunov, Eugene A., additional, Qu, Jihong, additional, Shlapakova, Iryna N., additional, Anyukhovsky, Evgeny P., additional, Liu, Lili, additional, Janse, Michiel J., additional, Brink, Peter R., additional, Cohen, Ira S., additional, Robinson, Richard B., additional, Danilo, Peter, additional, and Rosen, Michael R., additional

Published
2004
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20. Effect of Skeletal Muscle Na+ Channel Delivered Via a Cell Platform on Cardiac Conduction and Arrhythmia Induction.

Author

Boink, Gerard J. J., Jia Lu, Driessen, Helen E., Lian Duan, Sosunov, Eugene A., Anyukhovsky, Evgeny P., Shlapakova, Iryna N., Lau, David H., Rosen, Tove S., Danilo, Peter, Zhiheng Jia, Ozgen, Nazira, Bobkov, Yevgeniy, Guo, Yuanjian, Brink, Peter R., Kryukova, Yelena, Robinson, Richard B., Entcheva, Emilia, Cohen, Ira S., and Rosen, Michael R.

Subjects
MESENCHYMAL stem cells, SKELETAL muscle, SODIUM, SODIUM channels, HEART conduction system, ARRHYTHMIA
Abstract

The article discusses a study which examined the impact of canine mesenchymal stem cells (cMSC) in delivering skeletal muscle sodium (Na+) channel (SkM1). They involved the isolation and transfection of cMSCs with SkM1. The results revealed that cMSCs provide efficient delivery of SkM1 current and the interventions performed were neither antiarrhythmic nor proarrhythmic. The criticality of the delivery platform to SkM1 antiarrhythmic efficacy is also noted.

Published
2012
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23. Long-term cardiac memory in canine heart is associated with the evolution of a transmural repolarization gradient

Author

Coronel, Ruben, Opthof, Tobias, Plotnikov, Alexei N., Wilms-Schopman, Francien J.G., Shlapakova, Iryna N., Danilo, Peter, Sosunov, Eugene A., Anyukhovsky, Evgeny P., Janse, Michiel J., and Rosen, Michael R.

Subjects
DOGS, HETEROGENEITY, ENDOCARDIUM, ELECTROCARDIOGRAPHY
Abstract

Abstract: Objective: The contribution of regional electrophysiologic heterogeneity to the T-wave changes of long-term cardiac memory (CM) is not known. We mapped activation and repolarization in dogs after induction of CM and in sham animals. Methods and results: CM was induced by three weeks of AV-sequential pacing at the anterior free wall of the left ventricle (LV), midway between apex and base in 5 dogs. In 4 sham controls a pacemaker was implanted but ventricular pacing was not performed. At 3 weeks, unipolar electrograms were recorded (98 epicardial, 120 intramural and endocardial electrodes) during atrial stimulation (cycle length 450 ms). Activation times (AT) and repolarization times (RT) were measured and activation recovery intervals (ARIs) calculated. CM was associated with 1) deeper T waves on ECG, with no change in QT interval; 2) longer activation time at the site of stimulation in CM (29.7±1.0, X ±SEM) than sham (23.9±1.3 ms p <0.01); 3) an LV transmural gradient in repolarization time such that repolarization at the epicardium terminated 12.4±2.4 ms later than at the endocardium p <0.01), in contrast to no gradient in shams (2.7±4.2 ms); in memory dogs, the repolarization time gradient was greatest at sites around the pacing electrode varying from 13.1±2.3 ms to 25.5±3.8 ms; 4) more negative left ventricular potentials at the peak of the body surface T wave (−4.9±0.8 vs −2.2±0.4 mV; p <0.05) but no altered right ventricular epicardial T-wave potentials. ARIs did not differ between groups. Right ventricular activation was delayed but was not associated with altered repolarization because of compensatory shortening of the right ventricular ARIs. Conclusion: CM-induced T-wave changes are caused by evolution of transmural repolarization gradients manifested during atrial stimulation that are maximal near the site of ventricular pacing. [Copyright &y& Elsevier]

Published
2007
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24. Effect of Skeletal Muscle NaChannel Delivered Via a Cell Platform on Cardiac Conduction and Arrhythmia Induction

Author

Boink, Gerard J.J., Lu, Jia, Driessen, Helen E., Duan, Lian, Sosunov, Eugene A., Anyukhovsky, Evgeny P., Shlapakova, Iryna N., Lau, David H., Rosen, Tove S., Danilo, Peter, Jia, Zhiheng, Ozgen, Nazira, Bobkov, Yevgeniy, Guo, Yuanjian, Brink, Peter R., Kryukova, Yelena, Robinson, Richard B., Entcheva, Emilia, Cohen, Ira S., and Rosen, Michael R.

Abstract

In depolarized myocardial infarct epicardial border zones, the cardiac sodium channel is largely inactivated, contributing to slow conduction and reentry. We have demonstrated that adenoviral delivery of the skeletal muscle Nachannel (SkM1) to epicardial border zones normalizes conduction and reduces induction of ventricular tachycardiaventricular fibrillation. We now studied the impact of canine mesenchymal stem cells (cMSCs) in delivering SkM1.

Published
2012
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25. Effect of skeletal muscle Na(+) channel delivered via a cell platform on cardiac conduction and arrhythmia induction.

Author

Boink GJ, Lu J, Driessen HE, Duan L, Sosunov EA, Anyukhovsky EP, Shlapakova IN, Lau DH, Rosen TS, Danilo P, Jia Z, Ozgen N, Bobkov Y, Guo Y, Brink PR, Kryukova Y, Robinson RB, Entcheva E, Cohen IS, and Rosen MR

Subjects
Action Potentials, Animals, Animals, Newborn, Cardiac Pacing, Artificial, Cells, Cultured, Coculture Techniques, Disease Models, Animal, Dogs, Electrophysiologic Techniques, Cardiac, Humans, Muscle Proteins genetics, Myocardial Infarction complications, Myocardial Infarction genetics, Myocardial Infarction metabolism, Myocardial Infarction physiopathology, NAV1.5 Voltage-Gated Sodium Channel, Rats, Rats, Sprague-Dawley, Sodium Channels genetics, Tachycardia, Ventricular etiology, Tachycardia, Ventricular genetics, Tachycardia, Ventricular metabolism, Tachycardia, Ventricular physiopathology, Time Factors, Transfection, Ventricular Fibrillation etiology, Ventricular Fibrillation genetics, Ventricular Fibrillation metabolism, Ventricular Fibrillation physiopathology, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells metabolism, Muscle Proteins metabolism, Myocardial Infarction surgery, Myocytes, Cardiac metabolism, Sodium metabolism, Sodium Channels metabolism, Tachycardia, Ventricular prevention & control, Ventricular Fibrillation prevention & control
Abstract

Background: In depolarized myocardial infarct epicardial border zones, the cardiac sodium channel is largely inactivated, contributing to slow conduction and reentry. We have demonstrated that adenoviral delivery of the skeletal muscle Na(+) channel (SkM1) to epicardial border zones normalizes conduction and reduces induction of ventricular tachycardia/ventricular fibrillation. We now studied the impact of canine mesenchymal stem cells (cMSCs) in delivering SkM1., Methods and Results: cMSCs were isolated and transfected with SkM1. Coculture experiments showed cMSC/SkM1 but not cMSC alone and maintained fast conduction at depolarized potentials. We studied 3 groups in the canine 7d infarct: sham, cMSC, and cMSC/SkM1. In vivo epicardial border zones electrograms were broad and fragmented in sham, narrower in cMSCs, and narrow and unfragmented in cMSC/SkM1 (P<0.05). During programmed electrical stimulation of epicardial border zones, QRS duration in cMSC/SkM1 was shorter than in cMSC and sham (P<0.05). Programmed electrical stimulation-induced ventricular tachycardia/ventricular fibrillation was equivalent in all groups (P>0.05)., Conclusion: cMSCs provide efficient delivery of SkM1 current. The interventions performed (cMSCs or cMSC/SkM1) were neither antiarrhythmic nor proarrhythmic. Comparing outcomes with cMSC/SkM1 and viral gene delivery highlights the criticality of the delivery platform to SkM1 antiarrhythmic efficacy.

Published
2012
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