Search

Ferroelectric BaTiO3 with an electric-field-switchable spontaneous polarization has attracted wide attention in photovoltaic applications due to its efficient charge separation for photoexcitation. The evolution of its optical properties with rising temperature especially across the ferroelectric-paraelectric phase transition is critical to peer into the fundamental photoexcitation process. Herein, by combining spectroscopic ellipsometry measurements with first-principles calculations, we obtain the UV-Vis dielectric functions of perovskite BaTiO3 at temperatures varying from 300 to 873 K and provide the atomistic insights into the temperature-driven ferroelectric-paraelectric (tetragonal-cubic) structural evolution. The main adsorption peak in dielectric function of BaTiO3 is reduced by 20.6% in magnitude and redshifted as temperature increases. The Urbach tail shows an unconventional temperature-dependent behavior due to the microcrystalline disorder across the ferroelectric-paraelectric phase transition and the decreased surface roughness at around 405 K. From ab initio molecular dynamics simulations, the redshifted dielectric function of ferroelectric BaTiO3 coincidences with the reduction of the spontaneous polarization at elevated temperature. Moreover, a positive (negative) external electric field is applied which can modulate the dielectric function of ferroelectric BaTiO3 blueshift (redshift) with a larger (smaller) spontaneous polarization since it drives the ferroelectric further away from (closer to) the paraelectric structure. This work sheds light on the temperature-dependent optical properties of BaTiO3 and provides data support for advancing its ferroelectric photovoltaic applications.

Metal–organic frameworks (MOFs) have been extensively studied in many fields due to their abundant porous structures. The mechanism underlying the thermal conduction properties of MOFs, which plays an essential role in a wide variety of applications such as adsorbents and thermoelectric devices, remains elusive. It is also highly desirable to achieve the efficient modulation of thermal conductivity in MOFs via experimentally accessible methods such as metal substitution and strain engineering. In this work, we perform first-principles calculations to investigate the thermal transport properties of MOF-5, a representative prototype of MOFs. We find an ultralow thermal conductivity (κ) of 0.33 W m−1 K−1 at room temperature, in excellent agreement with the experimental measurement. Such ultralow κ is attributed to the strong phonon–phonon scattering that arises from the dense and intertwined low-frequency phonons. The phonon dispersion leads to unusual tuning strategies of κ, since conventional designing guidelines (e.g. substitution of heavier atoms or application of tensile strain is preferred in pursuit of lower thermal conductivity) are not fully obeyed in MOF-5. We find that isovalent substitutions of Zn atoms with (lighter) Mg and (heavier) Cd atoms both result in significant reduction of κ, due to the enhanced phonon scattering rates that are associated with the stronger bond strength and the larger atomic mass, respectively. We further demonstrate that the so-called “guitar string” vibrations are responsible for the anomalous non-monotonic variation of κ in MOF-5 under tensile strain. This work provides fundamental insights into the thermal transport mechanisms in MOF-5, which may have some important implications for the thermal management applications utilizing MOFs.

Background The aim of this meta-analysis was to explore associations between polymorphisms in CTLA-4/IL-18 and chronic liver diseases by combing the results of all relevant studies. Methods Eligible studies were searched from Pubmed, Embase, Web of Science and Cochrane library. We used Review Manager to combine the results of eligible studies. Results Sixty-seven studies were included in this meta-analysis. Combined results revealed that CTLA-4 rs231775 (dominant, recessive and allele comparisons), IL-18 rs1946518 (dominant, recessive and allele comparisons) and IL-18 rs187238 (dominant, over-dominant and allele comparisons) polymorphisms were all significantly associated with chronic liver diseases in the overall population. We also obtained similar positive results for rs231775, rs5742909, rs3087243, rs1946518 and rs187238 polymorphisms in subgroup analyses by ethnicity and type of disease. Conclusions This meta-analysis demonstrated that CTLA-4 rs231775, CTLA-4 rs5742909, CTLA-4 rs3087243, IL-18 rs1946518 and IL-18 rs187238 polymorphisms may confer susceptibility to certain types of chronic liver diseases.

Modified walnut shell (MWS) was obtained using diethylenetriamine through a grafting reaction and its adsorption capacity toward Cr(VI) was enhanced. The adsorbent was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and elemental analysis and the results showed that the modification was effective. To optimize experimental conditions, the effect of temperature, solution pH, salinity, contact time, and Cr(VI) concentration on adsorption quantity were performed in batch mode. It showed that the adsorption ability for Cr(VI) onto MWH can reach 50.1 mg·g−1 at 303 K with solution pH 3. Both the solution pH and salinity had a great impact on the adsorption capacity. The Langmuir model can predict the equilibrium process while the pseudo-second-order model can describe the kinetic process. The Yan model can be used to predict the column process. Additionally, there was also some regeneration ability for Cr-loaded MWH. Consequently, MWS is effective for removing Cr(VI) from solution.

Stretchable thin-film conductors are basic building blocks in advanced flexible and stretchable electronics. Current research mainly focuses on strategies to improve stretchability and widen the range of applications of stretchable conductors. However, stability should not be neglected, and the electrical failure mode is one of the most common stability issues that determines the current range and duration in a circuit. In this work, we report the electrical failure mechanism of stretchable conductors. We find a special failure mode for the stretchable conductors, which can be attributed to the coupling effect between local thermal strains and dynamic resistance changes of the thin film. This creates a vicious circle that significantly differs from traditional conductors. Physical parameters related to this special failure mode are investigated in detail. It is found that this mechanism is applicable to different kinds of stretchable conductors. Based on this finding, we also explore methods to modulate the failure of stretchable conductors. The failure mechanism found here provides a fundamental understanding of the current effect of stretchable circuits and is crucial for designing stable stretchable bioelectrodes and circuits.

Despite the extensive use of next-generation sequencing of RNA, simultaneous sequencing and quantitative mapping of multiple RNA modifications remain challenging. Herein, we develop MLC-Seq, a mass spectrometry-based direct sequencing method allowing for simultaneously unravelling the RNA sequences and quantitatively mapping different tRNA nucleotide modifications site-specifically. Importantly, MLC-Seq reveals the stoichiometric changes of tRNA modifications upon treatment with the dealkylating enzyme AlkB, and led to the discovery of new nucleotide modifications.

Poor anode performance is one of the main bottlenecks in the development of microbial fuel cells (MFCs) for practical applications. Multilayered Ti

Mass spectrometry (MS)-based sequencing has advantages in direct sequencing of RNA, compared to cDNA-based RNA sequencing methods, as it is completely independent of enzymes and base complementarity errors in sample preparation. In addition, it allows for sequencing of different RNA modifications in a single study, rather than just one specific modification type per study. However, many technical challenges remain in de novo MS sequencing of RNA, making it difficult to MS sequence mixed RNAs or to differentiate isomeric modifications such as pseudouridine (Ψ) from uridine (U). Our recent study incorporates a two-dimensional hydrophobic end labeling strategy into MS-based sequencing (2D-HELS MS Seq) to systematically address the current challenges in MS sequencing of RNA, making it possible to directly and de novo sequence purified single RNA and mixed RNA containing both canonical and modified nucleotides. Here, we describe the method to sequence representative single-RNA and mixed-RNA oligonucleotides, each with a different sequence and/or containing modified nucleotides such as Ψ and 5-methylcytosine (m

tRNAs can exist in distinct isoforms because of different chemical modifications, which confounds attempts to accurately sequence individual tRNA species using next generation sequencing approaches or to quantify different RNA modifications at specific sites on a tRNA strand. Herein, we develop a mass spectrometric (MS) ladder complementation sequencing (MLC-Seq), allowing for direct and simultaneous sequencing of full-length tRNA molecules, including those with low abundance. MLC-seq is achieved by improved instrumentation, and advanced algorithms that identify each tRNA species and related isoforms in an RNA mixture, and assemble full MS ladders from partial ladders with missing ladder components. Using MLC-Seq, we successfully obtained the sequence of tRNA-Phe from yeast and tRNA-Glu from mouse hepatocytes, and simultaneously revealed new tRNA isoforms derived from nucleotide modifications. Importantly, MLC-Seq pinpointed the location and stoichiometry changes of RNA modifications in tRNA-Glu upon the treatment of dealkylated enzyme AlkB, which confirmed its known enzymatic activity and suggested previously unidentified effects in RNA editing.

Microreactors are good platforms to study polymerization, but the production of high molecular weight polymers in small reaction space is rather difficult. To obtain million grams/mole molecular weight product, we developed two microreactors to implement inverse suspension polymerization in separated droplets, working as mini-reactors. Protecting by the lubricant film around droplets, 3600 kg/mol polyacrylamide (PAM) was successfully prepared in 38 min residence time, without any clogging issues. Advanced to the batch reactors, the microreactors offered precise control over the whole polymerization process in terms of reaction time and properties of the products, which facilitated the understanding of the basic laws of reactions. Thermal-initiated and redox-initiated radical polymerizations were both investigated, and the thermal-initiated reaction was found to be suitable for preparing high molecular weight polyacrylamide (>1000 kg/mol). The redox-initiated polymerization was a mixing-controlled reaction, which finished quickly in the microreactor.

Objective: The aim of this study was to compare the clinical outcomes of ex vivo liver resection and autotransplantation (ERAT) with allotransplantation in patients with end-stage hepatic alveolar echinococcosis (HAE). Methods: From January 2008 to October 2017, 41 of 254 patients with end-stage HAE fulfilled the inclusion criteria (ERAT group, n = 35; allotransplantation group, n = 6). Each group was assessed for indications and short- and long-term outcomes. Results: The intraoperative mortality was zero in both groups. Two patients died after ERAT due to intra-abdominal bleeding and acute cerebral hemorrhage. The most frequent postoperative complications were biliary complications, which occurred in six patients (14.6%). Parasite recurrence was recorded in two patients, at 16 months and 52 months after allotransplantation. The survival curve showed a longer survival time in the ERAT group than in the allotransplantation group (P = 0.028). In the ERAT group, the 1-, 3-, and 5-year overall survival rates were all 100%. In the allotransplantation group, the 1-, 3-, and 5-year overall survival rates were 100%, 83.3%, and 66.7%, respectively. Conclusions: This appears to be the first series comparing ERAT with allotransplantation for end-stage HAE. ERAT may be a superior alternative to allotransplantation in some cases, as it requires neither an organ donor nor immunosuppressive therapy. Keywords: Hepatic alveolar echinococcosis, Ex vivo, Resection, Autotransplantation, Allotransplantation, Immunosuppression

Mass spectrometry (MS)-based sequencing has advantages in direct sequencing of RNA, compared to cDNA-based RNA sequencing methods, as it is completely independent of enzymes and base complementarity errors in sample preparation. In addition, it allows for sequencing of different RNA modifications in a single study, rather than just one specific modification type per study. However, many technical challenges remain in de novo MS sequencing of RNA, making it difficult to MS sequence mixed RNAs or to differentiate isomeric modifications such as pseudouridine (Ψ) from uridine (U). Our recent study incorporates a two-dimensional hydrophobic end labeling strategy into MS-based sequencing (2D-HELS MS Seq) to systematically address the current challenges in MS sequencing of RNA, making it possible to directly and de novo sequence purified single RNA and mixed RNA containing both canonical and modified nucleotides. Here, we describe the method to sequence representative single-RNA and mixed-RNA oligonucleotides, each with a different sequence and/or containing modified nucleotides such as Ψ and 5-methylcytosine (m5C), using 2D-HELS MS Seq.

To increase the production of crops, chemical fertilizers are used in crop fields. However, underuse or overuse cannot increase crop yields but even decrease them and cause severe environmental problems. Thus, the detection and monitoring of chemical concentration are increasingly important. To build up and monitor a data-based system for a large area, such a method is costly and time-consuming. In this research, we developed a conductive polymer-based sensor to detect nitrate concentrations in soil water. Conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was used as our sensing material. To increase its conductivity, we used the vacuum phase polymerization method to achieve a high conductive and stable polymer film. The conductivity of the polymer film is 500 S/cm. Our results have demonstrated that the conductive polymer-based sensors have high sensitivity to nitrate solution. The response to 1000 ppm nitrate solution is 47.2% (Response = (Initrate - IDIwate) / IDIwater). The sensors can detect nitrate range from 1ppm to 1000 ppm. The response time is less than 1 minute. This impedance-based sensor will eventually be integrated with the surface acoustic wave sensors, combined with an antenna and a GPR unit for low maintenance, autonomous, and in-situ soil nutrient sensing.

Poor anode performance is one of the main bottlenecks in the development of microbial fuel cells (MFCs) for practical applications. Multilayered Ti3C2 MXene (m-MXene) is an alternative anode modification material because of its high specific surface area and electrical conductivity. However, the multilayered structure, negatively charged surface, and electropositivity of m-MXene could limit its modification effects. In this work, we used a solution-phase flocculation method (ammonium ion method) to restack and aggregate MXene nanosheets as an anode modification material (n-MXene). The n-MXene-modified anode had a higher specific surface area, surface hydrophilicity and surface electropositivity than the m-MXene-modified anode. The n-MXene-modified anode obtained a maximum current density of 2.1 A m−2, which was 31.2% and 61.5% higher than that of the m-MXene-modified anode (1.6 A m−2) and bare carbon fiber cloth anode (1.3 A m−2). This improved anode performance was attributed to both the decrease in the charge transfer resistance and diffusion resistance, which were related to the increased quantity of biomass and microbial nanowire (or pili)-shaped filaments on the electrode surface. This work provides a simple and cost-effective approach to prepare MXene nanosheets for the modification of MFC anodes.

Mass spectrometry (MS)-based sequencing approaches have been shown to be useful in direct sequencing RNA without the need for a complementary DNA (cDNA) intermediate. However, such approaches are rarely applied as a de novo RNA sequencing method, but used mainly as a tool that can assist in quality assurance for confirming known sequences of purified single-stranded RNA samples. Recently, we developed a direct RNA sequencing method by integrating a 2-dimensional mass-retention time hydrophobic end-labeling strategy into MS-based sequencing (2D-HELS MS Seq). This method is capable of accurately sequencing single RNA sequences as well as mixtures containing up to 12 distinct RNA sequences. In addition to the four canonical ribonucleotides (A, C, G, and U), the method has the capacity to sequence RNA oligonucleotides containing modified nucleotides. This is possible because the modified nucleobase either has an intrinsically unique mass that can help in its identification and its location in the RNA sequence, or can be converted into a product with a unique mass. In this study, we have used RNA, incorporating two representative modified nucleotides (pseudouridine (ψ) and 5-methylcytosine (m(5) C)), to illustrate the application of the method for the de novo sequencing of a single RNA oligonucleotide as well as a mixture of RNA oligonucleotides, each with a different sequence and/or modified nucleotides. The procedures and protocols described here to sequence these model RNAs will be applicable to other short RNA samples (

Post-transcriptional modifications are intrinsic to RNA structure and function. However, methods to sequence RNA typically require a cDNA intermediate and are either not able to sequence these modifications or aretailored to sequence one specific nucleotide modification only. Interestingly, some of these modifications occur with Phe without cDNA by integrating atwo-dimensional hydrophobic RNA end-labeling strategy with an anchor-based algorithm in mass spectrometry-based sequencing (2D-HELS-AA MS Seq). The entire tRNAPhe was sequenced and the identity, location, and stoichiometry of all eleven different RNA modifications was determined, five of which were not 100% modified, including a 2'-O-methylated G (Gm) in the wobble anticodon position as well as an N2, N2-dimethylguanosine (m22G), a7-methylguanosine (m7G), a1-methyladenosine (m1A), and awybutosine (Y), suggesting numerous post-transcriptional regulations in tRNA. Two truncated isoforms at the 3'-CCA tail of the tRNAPhe (75 nt with a 3'-CC tail (80% abundance) and 74 nt with a 3'-C tail (3% abundance)) were identified in addition to the full-length 3'-CCA-tailed tRNAPhe (76 nt, 17% abundance). We discovered a new isoform with A-G transitions/editing at the 44 and 45 positions in the tRNAPhe variable loop, and discuss possible mechanisms related to the emergence and functions of the isoforms with these base transitions or editing. Our method revealed new isoforms, base modifications, and RNA editing as well as their stoichiometries in the tRNA that cannot be determined by current cDNA-based methods, opening new opportunities in the field of epitranscriptomics.

Background Associations between polymorphisms in cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) / interleukin-18 (IL-18) and susceptibility to liver diseases were already reported by many publications. The aim of this meta-analysis was to clarify associations between polymorphisms in CTLA-4/IL-18 and liver diseases by combing the results of all relevant publications. Methods Eligible publications were searched from Pubmed, Embase, WOS and CNKI. The latest literature searching update was performed on 2nd October, 2019. We used Review Manager to combine the results of individual studies. Results Sixty-seven studies were included in this study. Combined results revealed that CTLA-4 rs231775 (dominant comparison: OR 0.83, 95 % CI 0.79-0.88; recessive comparison: OR 1.33, 95 % CI 1.23-1.43; allele comparison: OR 0.84, 95 % CI 0.78-0.90), IL-18 rs1946518 (dominant comparison: OR 0.85, 95 % CI 0.78-0.92; recessive comparison: OR 1.29, 95 % CI 1.13-1.48; allele comparison: OR 0.79, 95 % CI 0.71-0.88) and IL-18 rs187238 (dominant comparison: OR 1.28, 95 % CI 1.07-1.53; over-dominant comparison: OR 0.81, 95 % CI 0.68-0.97; allele comparison: OR 1.22, 95 % CI 1.07-1.39) polymorphisms were all significantly associated with liver diseases in the general population. We also obtained similar significant associations for CTLA-4 rs231775, CTLA-4 rs5742909, CTLA-4 rs3087243, IL-18 rs1946518 and IL-18 rs187238 polymorphisms in subgroup analyses. Conclusions Collectively, this meta-analysis proved that CTLA-4 rs231775, CTLA-4 rs5742909, CTLA-4 rs3087243, IL-18 rs1946518 and IL-18 rs187238 polymorphisms may confer susceptibility to various types of liver diseases.

In order to reduce the resistance and improve the hydrodynamic performance of a ship, two hull form design methods are proposed based on the potential flow theory and viscous flow theory. The flow fields are meshed using body-fitted mesh and structured grids. The parameters of the hull modification function are the design variables. A three-dimensional modeling method is used to alter the geometry. The Non-Linear Programming (NLP) method is utilized to optimize a David Taylor Model Basin (DTMB) model 5415 ship under the constraints, including the displacement constraint. The optimization results show an effective reduction of the resistance. The two hull form design methods developed in this study can provide technical support and theoretical basis for designing green ships.

The seakeeping behavior of a ship in waves is different from its behavior in calm water. The resistance and seakeeping performance of a ship are of great importance and must be considered in the early-stage design of a ship’s hull form design. Therefore, this paper proposes a hull form optimization framework aiming to achieve the minimum total resistance in waves using a CFD technique. A sinusoidal wave is adopted to establish the numerical wave tank and the overset mesh technique is used to facilitate the motions of the ships in question. Considering the motions of pitching and heaving, the total resistance of the hull in waves is regarded as the objective function which is calculated using the Reynolds averaged Navier–Stokes (RANS) method. The arbitrary shape deformation (ASD) technique is used to change the geometry. Under displacement and design variables, a hybrid algorithm is developed to evaluate the objective function combining the optimal Latin hypercube design (Opt LHD) and the non-linear programming by quadratic Lagrangian (NLPQL) algorithm. Finally, two examples of hull form optimization are presented and discussed for David Taylor Model Basin (DTMB) model 5512 and WIGLEY III cases. The results show the effectiveness of the optimization framework developed in the present study, which can lay the foundation for further optimization of full-scale ships.

Different seasonal health effects of ambient ozone (O3) have been reported in previous studies. This might be due to inappropriate adjustment of temperature in different seasons. We used daily data on non-accidental mortality and ambient air pollution in Zhengzhou from January 19, 2013 to June 30, 2015. Season-stratified analyses using generalized additive models were conducted to evaluate the seasonal associations with adjustment of temperature with different lagged days (lag0-1 for warm season, lag0-14 for cold season). We recorded a total of 70,443 non-accidental deaths in Zhengzhou during the study period. Significant associations were observed between ambient O3 and mortality in cold season. Every 10-μg/m3 increment of 24-h O3 of 1-day lagged time was associated with a 1.38% (95% CI 0.60, 2.16%) increase in all cause mortality, 1.35% (95% CI 0.41, 2.30%) increase in cardiovascular mortality, and 1.78% (95% CI 0.43, 3.14%) increase in respiratory mortality. Similar associations were observed when using daily 1- and 8-h maximum concentrations of O3. No significant association was found during warm season. This study suggests a more pronounced ozone-mortality association in cold season in Zhengzhou, and we suggest that different lagged temperatures should be considered when examining the seasonal health effects of ambient ozone.

We report a direct method for sequencing tRNAPhe without cDNA by combining 2-dimensional hydrophobic RNA end-labeling with an anchor-based algorithm in mass spectrometry-based sequencing (2D-HELS-AA MS Seq). The entire tRNAPhe was sequenced and the identity, location and abundance of all 11 base modifications were determined. Changes in ratios of wybutosine and its depurinated form under different conditions were quantified, pointing to the ability of our technology to determine dynamic changes of nucleotide modifications. Two truncated isoforms at 3’CCA tail of the tRNAPhe (75 nt CC, 80% and 74 nt C, 3%) were identified in addition to the 76 nt tRNAPhe with a full-length 3’CCA tail (17%). We also discovered a new isoform with A-G transitions at both the 44 and 45 positions in the tRNAPhe variable loop.One Sentence SummaryDirect 2D-HELS-AA MS Seq of tRNA reveals different isoforms and base modifications

A complete understanding of the structural and functional potential of RNA requires understanding of chemical modifications and noncanonical bases; this in turn requires advances in current sequencing methods to be able to sequence not only canonical ribonucleotides, but at the same time directly sequence these nonstandard moieties. Here, we present the first direct and modification type-independent RNA sequencing method via integration of a hydrophobic end-labeling strategy with of 2-D mass-retention time LC/MS analysis to allow de novo sequencing of RNA mixtures and enhance sample usage efficiency. Our method can directly read out the complete sequence, while identifying, locating, and quantifying base modifications accurately in both single and mixed RNA samples containing multiple different modifications at single-base resolution. Our method can also quantify stoichiometry/percentage of modified RNA vs. its canonical counterpart RNA, simulating a real biological sample where modifications exist but may not be 100% at a particular site of the RNA. This method is a critical step towards fully sequencing real complex cellular RNA samples of any type and containing any modification types and can also be used in the quality control of modified therapeutic RNAs.

Alkaline basalts are common constituents in many ophiolite belts and accretionary complexes. Here we report geological, geochronological, geochemical, and whole-rock Sr–Nd isotopic data for alkaline basalts from the Karamay ophiolitic melange of West Junggar, southern Central Asian Orogenic Belt (CAOB). In the ophiolitic melange, imbricate thrusts, duplexes, “web” structures, pinch-and-swell structures, tilted structures in pillow metabasalts, and shear band cleavages are widely developed. U–Pb analyses of zircon grains from a representative basalt by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) yielded crystallization age of ca. 395 ± 3 Ma, suggesting formation during the Middle Devonian. Geochemically, all the samples bear the signature of ocean island basalt (OIB), and are characterized by alkaline affinity with high concentrations of Na2O + K2O (4.22–7.28 wt.%) and TiO2 (1.72–2.89 wt.%), LILE and LREE enrichment and HREE depletion ((La/Yb)N = 8.4–15.6), with very strong or no Eu anomalies (Eu/Eu* = 0.6–1.1), and no obvious Nb, Ta or Ti negative anomalies. The rocks display consistent Sr–Nd isotopic compositions (initial 87Sr/86Sr ratios = 0.70326–0.70453, eNd(t) = + 3.1–+7.6). The results suggest that all the alkaline basalts were derived from a mantle plume-related source in an intra-oceanic setting with ca. 1–3% degree partial melting of garnet lherzolite. These observations, in combination with previous work, indicate that the alkaline basalts from Karamay ophiolitic melange can be correlated to a Middle Devonian mantle plume-related magmatism within Paleo-Asian Ocean. We further suggest that the plume-related activity in the CAOB was generally continuous during the development of the Paleo-Asian Ocean.

Mass spectrometry (MS) is a powerful technique for characterizing noncanonical nucleobases and other chemical modifications in small RNAs, yielding rich chemical information that is complementary to high-throughput indirect sequencing. However, mass spectra are often prohibitively complex when fragment ions are analyzed following either solution phase hydrolysis or gas phase fragmentation. For all but the simplest cases, ions arising from multiple fragmentation events, alternative fragmentation pathways, and diverse salt adducts frequently obscure desired single-cut fragment ions. Here we show that it is possible to take advantage of predictable regularities in liquid chromatographic (LC) separation of optimized RNA digests to greatly simplify the interpretation of complex MS data. A two-dimensional analysis of extracted compound chromatograms permits straightforward and robust de novo sequencing, using a novel Monte Carlo algorithm that automatically generates bidirectional paired-end reads, pinpointing the position of modified nucleotides in a sequence. We demonstrate that these advances permit routine LC-MS sequencing of RNAs containing noncanonical nucleotides, and we furthermore examine the applicability of this approach to the study of oligonucleotides containing artificial modifications as well as those commonly observed in post-transcriptionally modified RNAs.

In order to accurately predict the erosion effect of underwater cleaning with an angle nozzle under different working conditions, this paper uses refractory bricks to simulate marine fouling as the erosion target, and studies the optimized erosion prediction model by erosion test based on the submerged low-pressure water jet. The erosion test is conducted by orthogonal experimental design, and experimental data are used for the prediction model. By combining with statistical range and variance analysis methods, the jet pressure, impact time and jet angle are determined as three inputs of the prediction model, and erosion depth is the output index of the prediction model. A virtual data generation method is used to increase the amount of input data for the prediction model. This paper also proposes a Mind-evolved Advanced Genetic Algorithm (MAGA), which has a reliable optimization effect in the verification of four stand test functions. Then, the improved back-propagating (BP) neural network prediction models are established by respectively using Genetic Algorithm (GA) and MAGA optimization algorithms to optimize the initial thresholds and weights of the BP neural network. Compared to the prediction results of the BP and GA-BP models, the R2 of the MAGA-BP model is the highest, reaching 0.9954, the total error is reduced by 47.31% and 35.01%, the root mean square error decreases by 51.05% and 31.80%, and the maximum absolute percentage error decreases by 65.79% and 64.01%, respectively. The average prediction accuracy of the MAGA-BP model is controlled within 3%, which has been significantly improved. The results show that the prediction accuracy of the MAGA-BP prediction model is higher and more reliable, and the MAGA algorithm has a good optimization effect. This optimized erosion prediction method is feasible.