33 results on '"Sheng-Lin Qiao"'
Search Results
2. Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions
- Author
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Li-Li Li, Sheng-Lin Qiao, Wei-Jiao Liu, Yang Ma, Dong Wan, Jie Pan, and Hao Wang
- Subjects
Science - Abstract
The intracellular topology of a nanostructure plays a major role in its interactions with the cell and accordingly, its biological applications. Here, the authors design peptides that intracellularly polymerize into elastin-like polypeptides and assemble into various topologies, each of which exhibits a distinct set of biological functions.
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- 2017
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3. mRNA therapeutics for disease therapy: principles, delivery, and clinical translation
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Da-Wei Zhou, Ke Wang, Ying-Ao Zhang, Ke Ma, Xiao-Chun Yang, Zhen-Yi Li, Shou-Shan Yu, Ke-Zheng Chen, and Sheng-Lin Qiao
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Biomedical Engineering ,General Materials Science ,General Chemistry ,General Medicine - Abstract
This review provides an overview of the latest research progress in mRNA structural optimization strategies and their delivery systems, and discusses key considerations for their future clinical use.
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- 2023
4. CuCoFe Layered double hydroxides as laccase mimicking nanozymes for colorimetric detection of pheochromocytoma biomarkers
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Feng-Wei Huang, Ke Ma, Xiu-Wen Ni, Sheng-Lin Qiao, and Ke-Zheng Chen
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L-Lactate Dehydrogenase ,Iron ,Laccase ,Molecular Mimicry ,Adrenal Gland Neoplasms ,Metals and Alloys ,Cobalt ,Pheochromocytoma ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomarkers, Tumor ,Hydroxides ,Materials Chemistry ,Ceramics and Composites ,Humans ,Colorimetry ,Smartphone ,Copper - Abstract
A laccase catalyzed colorimetric biosensing approach is promising for the detection of pheochromocytoma biomarkers, yet suffers from the poor stability of enzymes and high cost for production. Here we report for the first time an easy to produce, cheap, stable and reliable laccase-mimicking CuCoFe-LDHzyme, which can catalyze the oxidation of pheochromocytoma biomarkers to form a chromogenic product for smartphone-based colorimetric detection.
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- 2022
5. A photocontrollable thermosensitive chemical spatiotemporally destabilizes mitochondrial membranes for cell fate manipulation
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Xiu-Wen Ni, Ke-Zheng Chen, and Sheng-Lin Qiao
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Polymers ,Mitochondrial Membranes ,Biomedical Engineering ,General Materials Science ,Apoptosis ,Peptides ,Mitochondria - Abstract
Perturbations in mitochondrial membrane stability lead to cytochrome c release and induce caspase-dependent apoptosis. Using synthetic smart chemicals with changeable physicochemical properties to interfere the mitochondrial membrane stability has not yet been reported. Here we show that a thermosensitive anchor-polymer-peptide conjugate (anchor-PPC) destabilizes mitochondrial membranes upon
- Published
- 2022
6. Point-of-care non-invasive enzyme-cleavable nanosensors for acute transplant rejection detection
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Shi-Jie Liu, Ke Ma, Long-Shan Liu, Ke Wang, Ying-Ao Zhang, Zi-Rong Bi, Yan-Xu Chen, Ke-Zheng Chen, Chang-Xi Wang, and Sheng-Lin Qiao
- Subjects
Graft Rejection ,Mice ,Point-of-Care Systems ,Electrochemistry ,Biomedical Engineering ,Biophysics ,Animals ,Biosensing Techniques ,Gold ,General Medicine ,Kidney Transplantation ,Biomarkers ,Biotechnology - Abstract
Accurate and non-invasive monitoring of allograft posttransplant is essential for early detection of acute cellular rejection and determines the long-term survival of the graft. Clinically, tissue biopsy is the most effective approach for diagnosing transplant rejection. Nonetheless, the procedure is invasive and potentially triggers organ failure. This work aims to design and apply GzmB-responsive nanosensors (GBRNs) that can readily size-change in graft tissues. Subsequently, we investigate the activity of serine protease granzyme B by generating a direct colorimetric urinary readout for non-invasive detection of transplant rejection in under 1 h. In preclinical heart graft mice models of transplant rejection, GBRNs were cleaved by GzmB and excreted by the kidneys via accurate nanometre-size glomerular filtration. By exploiting the catalytic activity of ultrasmall gold nanoclusters, GBRNs urinalysis promotes ultrasensitive surveillance of rejection episodes with a receiver operator characteristic curve area under the curve of 0.896 as well as a 95% confidence interval of about 0.7701-1.000. Besides, the catalytic activity of gold nanoclusters in urine can be detected at point-of-care testing to predict the immunity responses in mice with insufficient immunosuppressive therapy. Therefore, this non-invasive, sensitive, and quantitative method is a robust and informative approach for rapid and routine monitoring of transplant allografts without invasive biopsy.
- Published
- 2022
7. In situ phase transitional polymeric vaccines for improved immunotherapy
- Author
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Yi Wang, Sheng-Lin Qiao, Jie Wang, Muhetaerjiang Mamuti, Hao Wang, and Hong-Wei An
- Subjects
In situ ,Multidisciplinary ,Materials science ,medicine.medical_treatment ,Phase (matter) ,medicine ,Nanotechnology ,Immunotherapy - Abstract
Cancer vaccines have exhibited immense potential in cancer treatment. Through activating the host's immune system, vaccines stimulate extensive functional T cells to eliminate cancer. However, the therapeutic efficacy of cancer vaccines is limited by their inferior lymph node delivery and inadequate uptake of dendritic cells. Herein, we propose an in situ phase transitional strategy on vaccine manufacturing to maximally enhance lymph node drainage while ensuring adequate dendritic cell uptake. The phase transitional vaccines, with dynamic size modulation property, retain a small size (24.4 ± 3.1 nm) during lymph node draining then transform into larger particles (483.0 ± 41.6 nm) on-site by external signal input. Results show that this strategy induced rapid and robust immune response in a mouse melanoma tumor model. Furthermore, a stronger humoral immune response was observed in mice when immunized with MHC-II restricted antigen, which demonstrated that lymph node-targeted cancer vaccine delivery could be effectively manipulated through dynamic size modulation.
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- 2021
8. Thermoresponsive Polymer Assemblies: From Molecular Design to Theranostics Application
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Sheng-Lin Qiao, Muhetaerjiang Mamuti, Hong-Wei An, and Hao Wang
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Polymers and Plastics ,Organic Chemistry ,Materials Chemistry ,Ceramics and Composites ,Surfaces and Interfaces - Published
- 2022
9. In Situ Manipulation of Dendritic Cells by an Autophagy-Regulative Nanoactivator Enables Effective Cancer Immunotherapy
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Yuying Liu, Bo Huang, Junqing Wang, Wenqian Dong, Hao Wang, Hong-Wei An, Yao-Xin Lin, Sheng-Lin Qiao, Yi Wang, Muhetaerjiang Mamuti, Jie Wang, Lei Wang, and Chao Yang
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Chemistry ,medicine.medical_treatment ,T cell ,Antigen presentation ,General Engineering ,General Physics and Astronomy ,Priming (immunology) ,02 engineering and technology ,Dendritic cell ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Tumor antigen ,0104 chemical sciences ,medicine.anatomical_structure ,Cancer immunotherapy ,Antigen ,medicine ,Cancer research ,General Materials Science ,0210 nano-technology ,Ex vivo - Abstract
Cellular immunotherapeutics aim to employ immune cells as anticancer agents. Ex vivo engineering of dendritic cells (DCs), the initial role of an immune response, benefits tumor elimination by boosting specific antitumor responses. However, directly activating DCs in vivo is less efficient and therefore quite challenging. Here, we designed a nanoactivator that manufactures DCs through autophagy upregulating in vivo directly, which lead to a high-efficiency antigen presention of DCs and antigen-specific T cells generation. The nanoactivator significantly enhances tumor antigen cross-presentation and subsequent T cell priming. Consequently, in vivo experiments show that the nanoactivators successfully reduce tumor growth and prolong murine survival. Taken together, these results indicate in situ DCs manipulation by autophagy induction is a promising strategy for antigen presentation enhancement and tumor elimination.
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- 2019
10. Nanoantagonists with nanophase-segregated surfaces for improved cancer immunotherapy
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Hao Wang, Yi Wang, Hong-Wei An, Yang Ma, Yao-Xin Lin, Lei Wang, Sheng-Lin Qiao, and Xiaochun Wu
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Polymers ,Surface Properties ,T cell ,medicine.medical_treatment ,Programmed Cell Death 1 Receptor ,Melanoma, Experimental ,Biophysics ,Antineoplastic Agents ,Bioengineering ,Peptide ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,B7-H1 Antigen ,Biomaterials ,Immune system ,Cancer immunotherapy ,Neoplasms ,medicine ,Animals ,Tissue Distribution ,chemistry.chemical_classification ,Chemistry ,Temperature ,Immunotherapy ,021001 nanoscience & nanotechnology ,Immune checkpoint ,0104 chemical sciences ,Cell biology ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Mechanics of Materials ,Blood Circulation ,Cancer cell ,Ceramics and Composites ,Nanoparticles ,Female ,Adsorption ,Peptides ,0210 nano-technology ,Protein Binding ,Protein adsorption - Abstract
The blockade of PD-1/PD-L1 interaction by peptide antagonists can unleash and enhance pre-existing anti-cancer immune responses of T cells to eradicate cancer cells. However, low proteolytic stability is the “Achilles' Heel” of peptides. Here, we first report a nanoantagonist with a physiological temperature sensitive nanophase-segregated surface that exhibits significantly enhanced blood circulation, peptide stability and PD-L1 immune checkpoint blockade efficacy. Thermosensitive polymers with different phase transition temperatures (Tt) are used to form the nanophase-segregated surface on an Au nanorod core. Importantly, the nanophase-segregated surface aids the nanoantagonist to resist protein adsorption and enhance the systemic stability of the linked peptides. Finally, the as-designed nanoantagonist effectively blocks PD-1/PD-L1 interaction in vitro and in vivo, enhances the pre-existing CD8+ T cell tumor destruction capability and inhibits tumor growth. This study offers a new strategy for designing nano-formulations for cancer immunotherapy.
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- 2018
11. Facile Synthesis of Peptide Cross-Linked Nanogels for Tumor Metastasis Inhibition
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Yi Wang, Hao Wang, and Sheng-Lin Qiao
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Cell invasion ,chemistry.chemical_classification ,MMP2 ,010405 organic chemistry ,Peptide ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,In vitro ,0104 chemical sciences ,Metastasis ,chemistry ,In vivo ,Biophysics ,Precipitation polymerization ,medicine ,General Materials Science ,0210 nano-technology ,Nanogel - Abstract
Cancer metastasis is one of the major factors contributing to high mortality, and here we designed and synthesized a peptide cross-linked nanogel for hydrophilic functional molecule encapsulation and tumor metastasis inhibition. In this investigation, matrix metalloproteinase-2 (MMP2)-responsive peptide cross-linker is prepared by coupling acrylate-functional groups at both peptide terminals. The peptide cross-linked nanogel is then synthesized by free radical precipitation polymerization using N-isopropylacrylamide as the scaffold. Meanwhile, the hydrophilic tumor metastasis inhibition peptide P-5m is encapsulated into the nanogel in the one-pot synthesis step without complex cooperation. Importantly, the size-uniformed and narrow-distributed nanogel exhibits high P-5m loading capacity (43.4%). Interestingly, the nanogel is stable in vitro and in vivo, showing excellent and specific MMP2-triggered payload release property. As expected, the nanogel suppresses cancer cell migration and invasion in high per...
- Published
- 2018
12. General Approach of Stimuli-Induced Aggregation for Monitoring Tumor Therapy
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Li-Li Li, Yang Ma, Yao-Xin Lin, Hong-Wei An, Sheng-Lin Qiao, Hao Wang, and Yi Wang
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Phase transition ,Materials science ,Polymers ,Mice, Nude ,General Physics and Astronomy ,Peptide ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Phase Transition ,Mice ,Protein Aggregates ,Neoplasms ,Animals ,Humans ,Molecule ,General Materials Science ,chemistry.chemical_classification ,Mice, Inbred BALB C ,Antibiotics, Antineoplastic ,Microscopy, Confocal ,Optical Imaging ,Temperature ,General Engineering ,Tumor therapy ,Polymer ,Prognosis ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,chemistry ,Doxorubicin ,Disease Progression ,MCF-7 Cells ,Female ,Self-assembly ,Peptides ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions ,Intracellular ,Conjugate - Abstract
Intracellular construction of nanoaggregates from synthetic molecules to mimic natural ordered superstructures has gained increasing attention recently. Here, we develop an endogenous stimuli-induced aggregation (eSIA) approach to construct functional nanoaggregates for sensing and monitoring cellular physiological processes in situ. We design a series of thermosensitive polymer-peptide conjugates (PPCs), which are capable of constructing nanoaggregates in cells based on their isothermal phase transition property. The PPCs are composed of three moieties (i.e., a thermoresponsive polymer backbone, a grafted peptide, and a signal-molecule label). The bioenvironment-associated phase transition behavior of PPCs are carefully studied by consideration of various crucial parameters such as chain length, hydrophilicity, ratio of grafted peptides, and concentration. Intriguingly, under the specific intracellular stimulus, the PPCs are tailored and simultaneously form nanoaggregates exhibiting long-term retention effect, which enables specific identification and quantification of endogenous factors. This general approach is expected for high-performance in situ sensing and dynamic monitoring of disease progression in living subjects.
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- 2017
13. A tumour-selective cascade activatable self-detained system for drug delivery and cancer imaging
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Yuliang Zhao, Sheng Lin Qiao, Yong Cong, Wanhai Xu, Man Di Wang, Ziqi Wang, Wen Ting Chen, Qian Cai, Li-Li Li, Hong-Wei An, Chu Qi Lu, Chao Yang, Xiao Xiao Zhao, Yi Wang, Yang Ma, Yao-Xin Lin, Hao Wang, and Dayong Hou
- Subjects
Cancer therapy ,Amino Acid Motifs ,Cell ,General Physics and Astronomy ,02 engineering and technology ,Kidney ,Protein Engineering ,01 natural sciences ,Mice ,Drug Delivery Systems ,lcsh:Science ,Coloring Agents ,Antibiotics, Antineoplastic ,Multidisciplinary ,Chemistry ,Mononuclear phagocyte system ,Carbocyanines ,021001 nanoscience & nanotechnology ,Small molecule ,XIAP ,medicine.anatomical_structure ,Liver ,Drug delivery ,0210 nano-technology ,Science ,Biological Availability ,010402 general chemistry ,Sensitivity and Specificity ,Article ,General Biochemistry, Genetics and Molecular Biology ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Carcinoma, Transitional Cell ,Bladder cancer ,General Chemistry ,medicine.disease ,Xenograft Model Antitumor Assays ,0104 chemical sciences ,HEK293 Cells ,Urinary Bladder Neoplasms ,Doxorubicin ,Cancer cell ,Cancer research ,lcsh:Q ,Peptides ,Neoplasm Transplantation - Abstract
Achieving the activation of drugs within cellular systems may provide targeted therapies. Here we construct a tumour-selective cascade activatable self-detained system (TCASS) and incorporate imaging probes and therapeutics. We show in different mouse models that the TCASS system accumulates in solid tumours. The molecules show enhanced accumulation in tumour regions via the effect of recognition induced self-assembly. Analysis of the molecular penetration in tumour tissue shows that in vivo self-assembly increases the penetration capability compared to typical soft or hard nanomaterials. Importantly, the in vivo self-assembled molecules exhibit a comparable clearance pathway to that of small molecules, which are excreted from organs of the reticuloendothelial system (liver and kidney), while are relatively slowly eliminated from tumour tissues. Finally, this system, combined with the NIR probe, shows high specificity and sensitivity for detecting bladder cancer in isolated intact patient bladders., The activation of drugs within cellular systems may provide targeted therapies for cancer. Here, the authors make a drug delivery system that is activated within the cell and exploits XIAP expression to cleave a linker region, resulting in the self-assembly of the system and drug release within cancer cells.
- Published
- 2019
14. Thermoresponsive Polymeric Assemblies and Their Biological Applications
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Hao Wang and Sheng-Lin Qiao
- Subjects
Key factors ,Phase transition temperature ,Materials science ,Molecular level ,Thermoresponsive polymers in chromatography ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,0210 nano-technology ,01 natural sciences ,humanities ,0104 chemical sciences - Abstract
Thermoresponsive polymeric assemblies are fabricated from thermoresponsive polymers based on the soluble to insoluble transition at the phase transition temperature. The architecture of the self-assemblies lies in the molecular level and influenced by the structural change of the thermoresponsive polymers. The formed nanoassemblies endow excellent biofunctions for biomedical applications. Typical thermoresponsive polymers are reviewed in this chapter. The design concept and key factors to influence the thermal behavior are also discussed and summarized. As the sprouting and promising field, in vivo self-assembled thermoresponsive polymeric assemblies are also discussed in this chapter.
- Published
- 2018
15. Intracellular construction of topology-controlled polypeptide nanostructures with diverse biological functions
- Author
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Jie Pan, Wei-Jiao Liu, Sheng-Lin Qiao, Hao Wang, Dong Wan, Yang Ma, and Li-Li Li
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Nanostructure ,Science ,General Physics and Astronomy ,Biocompatible Materials ,Peptide ,macromolecular substances ,02 engineering and technology ,010402 general chemistry ,Topology ,01 natural sciences ,Article ,General Biochemistry, Genetics and Molecular Biology ,Polymerization ,Extracellular matrix ,Humans ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,Temperature ,General Chemistry ,021001 nanoscience & nanotechnology ,Biocompatible material ,Elastin ,Nanostructures ,0104 chemical sciences ,Cell biology ,chemistry ,MCF-7 Cells ,Nanoparticles ,lcsh:Q ,Peptides ,0210 nano-technology ,Intracellular ,HeLa Cells - Abstract
Topological structures of bio-architectonics and bio-interfaces play major roles in maintaining the normal functions of organs, tissues, extracellular matrix, and cells. In-depth understanding of natural self-assembly mechanisms and mimicking functional structures provide us opportunities to artificially control the natural assemblies and their biofunctions. Here, we report an intracellular enzyme-catalyzed polymerization approach for efficient synthesis of polypeptides and in situ construction of topology-controlled nanostructures. We reveal that the phase behavior and topological structure of polypeptides are encoded in monomeric peptide sequences. Next, we elucidate the relationship between polymerization dynamics and their temperature-dependent topological transition in biological conditions. Importantly, the linearly grown elastin-like polypeptides are biocompatible and aggregate into nanoparticles that exhibit significant molecular accumulation and retention effects. However, 3D gel-like structures with thermo-induced multi-directional traction interfere with cellular fates. These findings allow us to exploit new nanomaterials in living subjects for biomedical applications., The intracellular topology of a nanostructure plays a major role in its interactions with the cell and accordingly, its biological applications. Here, the authors design peptides that intracellularly polymerize into elastin-like polypeptides and assemble into various topologies, each of which exhibits a distinct set of biological functions.
- Published
- 2017
16. Photothermal Ring Integrated Intraocular Lens for High‐Efficient Eye Disease Treatment
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Yi Wang, Jiang-Bing Xie, Yang Zhao, Yongsheng Li, Jiao Yan, Yu-Juan Gao, Yao-Xin Lin, Xiaochun Wu, Chao Yang, Pei-Pei Yang, Zeng-Ying Qiao, Xue-Feng Hu, Lei Wang, Siquan Zhu, Sheng-Lin Qiao, Xin-Ce Sui, Li-Li Li, and Hao Wang
- Subjects
Laser surgery ,medicine.medical_specialty ,Materials science ,Eye Diseases ,genetic structures ,Biocompatibility ,medicine.medical_treatment ,Eye disease ,Acrylic Resins ,Intraocular lens ,02 engineering and technology ,03 medical and health sciences ,0302 clinical medicine ,Optics ,Lens Implantation, Intraocular ,Ophthalmology ,medicine ,Animals ,General Materials Science ,Posterior capsule opacification ,Lenses, Intraocular ,Eye, Artificial ,business.industry ,Mechanical Engineering ,Photothermal effect ,Photothermal therapy ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,medicine.disease ,eye diseases ,medicine.anatomical_structure ,Mechanics of Materials ,Lens (anatomy) ,030221 ophthalmology & optometry ,Rabbits ,sense organs ,0210 nano-technology ,business - Abstract
Posterior capsule opacification (PCO) is the most common complication after cataract surgery. So far, the only method for PCO treatment is the precisely focused laser surgery. However, it causes severe complications such as physical damages and neuron impairments. Here, a nanostructured photothermal ring integrated intraocular lens (Nano-IOLs) is reported, in which the rim of commercially available IOLs (C-IOLs) is decorated with silica coated Au nanorods (Au@SiO2 ), for high-efficient prevention of PCO after cataract surgery. The Nano-IOLs is capable of eliminating the residual lens epithelial cells (LECs) around Nano-IOLs under mild laser treatment and block the formation of disordered LECs fibrosis, which eventually leads to the loss of vision. The Nano-IOLs shows good biocompatibility as well as extraordinary region-confined photothermal effect. In vivo studies reveal that PCO occurrence in rabbit models is about 30%-40% by using Nano-IOLs, which is significantly lower than the control group that treated with C-IOLs (100% PCO occurrence) 30 d postsurgery. To the best of our knowledge, it is the first example to integrate nanotechnology with intraocular implants aiming to clinically relevant PCO. Our findings indicate that spatial controllability of photothermal effect from nanomaterials may provide a unique way to intervene the PCO-induced loss of vision.
- Published
- 2017
17. 'In vivo self-assembled' nanoprobes for optimizing autophagy-mediated chemotherapy
- Author
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Sheng-Lin Qiao, Yao-Xin Lin, Yang Ma, Lei Wang, Yi Wang, Hao Wang, Hong-Wei An, and Jie Wang
- Subjects
0301 basic medicine ,Porphyrins ,medicine.medical_treatment ,Biophysics ,Nanoprobe ,Photoacoustic imaging in biomedicine ,Autophagy-Related Proteins ,Bioengineering ,Antineoplastic Agents ,Biology ,Self assembled ,Biomaterials ,Photoacoustic Techniques ,03 medical and health sciences ,Mice ,In vivo ,Neoplasms ,medicine ,Autophagy ,Polyamines ,Animals ,Humans ,Disease treatment ,Chemotherapy ,Mice, Inbred BALB C ,Cell biology ,Cysteine Endopeptidases ,030104 developmental biology ,Systemic toxicity ,Mechanics of Materials ,Immunology ,Ceramics and Composites ,MCF-7 Cells ,Female - Abstract
Autophagic therapy is regarded as a promising strategy for disease treatment. Appropriate autophagy regulations in vivo play a crucial role in translating this new concept from benchside to bedside. So far, emerging technologies are required to spatially and quantitatively monitor autophagic process in vivo in order to minimize the cytotoxity concerns associated with autophagy-mediated therapy. We successfully demonstrate the "proof-of-concept" study on autophagy-mediated chemotherapy in mice. Here, we describe a photoacoustic (PA) nanoprobe based on "in vivo self-assembly" idea for real-time and quantitative detection of autophagy in mice for the first time. The purpurin-18 (P18) monomer is connected to hydrophilic poly(amidoamine) dendrimer (4th generation) through a peptide (GKGSFGFTG) that can be cleaved by an autophagy-specific enzyme, i.e., ATG4B, consequently resulting in aggregation of P18 and enhanced PA signals. Based on this aggregation-induced "turn-on" PA signals, we noninvasively determine the ATG4B activity for monitoring autophagy of tumor in vivo. According to the results of PA imaging, we could optimize chemotherapy efficacy through precisely modulating autophagy, which thereby decrease systemic toxicity from chemotherapeutics and autophagy inhibitors. We envision it will pave the way for developing autophagy-based treatment of diseases in the future.
- Published
- 2017
18. An in Situ Intracellular Self-Assembly Strategy for Quantitatively and Temporally Monitoring Autophagy
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Yi Wang, Hong-Wei An, Yang Ma, Sheng-Lin Qiao, Zeng-Ying Qiao, Ruo-Xin Zhang, Lei Wang, R. P. Yeshan J. Rajapaksha, Yao-Xin Lin, and Hao Wang
- Subjects
0301 basic medicine ,In situ ,Cell signaling ,General Physics and Astronomy ,Peptide ,Biology ,03 medical and health sciences ,Dendrimer ,Cell Line, Tumor ,Autophagy ,Animals ,Humans ,General Materials Science ,Zebrafish ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,General Engineering ,Temperature ,Fluorescence ,030104 developmental biology ,Biochemistry ,chemistry ,Biophysics ,MCF-7 Cells ,Linker ,Oligopeptides ,Intracellular ,HeLa Cells - Abstract
Autophagy plays a crucial role in the metabolic process. So far, conventional methods are incapable of rapid, precise, and real-time monitoring of autophagy in living objects. Herein, we describe an in situ intracellular self-assembly strategy for quantitative and temporal determination of autophagy in living objectives. The intelligent building blocks (DPBP) are composed by a bulky dendrimer as a carrier, a bis(pyrene) derivative (BP) as a signal molecule, and a peptide linker as a responsive unit that can be cleaved by an autophagy-specific enzyme, i.e., ATG4B. DPBP maintains the quenched fluorescence with monomeric BP. However, the responsive peptide is specifically tailored upon activation of autophagy, resulting in self-aggregation of BP residues which emit a 30-fold enhanced fluorescence. By measuring the intensity of fluorescent signal, we are able to quantitatively evaluate the autophagic level. In comparison with traditional techniques, such as TEM, Western blot, and GFP-LC3, the reliability and accuracy of this method are finally validated. We believe this in situ intracellular self-assembly strategy provides a rapid, effective, real-time, and quantitative method for monitoring autophagy in living objects, and it will be a useful tool for autophagy-related fundamental and clinical research.
- Published
- 2017
19. One-pot synthesis of pH-sensitive poly(RGD-co-β-amino ester)s for targeted intracellular drug delivery
- Author
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Hao Wang, Zeng-Ying Qiao, Yu Chen, Yunshan Fan, Sheng-Lin Qiao, and Gang Fan
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Polymers and Plastics ,Tertiary amine ,Chemistry ,Organic Chemistry ,technology, industry, and agriculture ,Nile red ,Nanoparticle ,Bioengineering ,Propylamine ,Biochemistry ,chemistry.chemical_compound ,Dynamic light scattering ,Polymer chemistry ,Copolymer ,Titration ,Ethylene glycol - Abstract
We report a convenient synthetic approach for one-pot preparation of poly(β-amino ester)s copolymerized with peptides. A family of copolymers with tertiary amine groups was synthesized by copolymerizing di(ethylene glycol) diacrylate (DEDA), poly(ethylene glycol) diacrylate (PEGDA), 3-(diethylamino)propylamine (DEPA) and GGRGD peptides using the Michael addition reaction. The hydrophobic/hydrophilic properties of the resulting copolymers are adjusted by altering the feed ratios of DEDA and PEGDA. The copolymers self-assembled into nanoparticles with sizes around 60–140 nm in aqueous media, which were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) techniques. The copolymers exhibit pH sensitive properties upon introduction of DEPA moieties, which were proved by pyrene fluorescence and pH titration measurements. The acid-triggered dissociation behaviors of the nanoparticles were studied by DLS and Nile red (NR) release experiments, revealing that the sizes of dissociated copolymer nanoparticles were closely relevant to their compositions. The nanoparticles can load the hydrophobic anticancer drug, i.e., doxorubicin (DOX). The DOX-loaded nanoparticles were stable in a neutral phosphate buffer solution with a payload leakage less than 20% at 37 °C. However, a significant acid-triggered DOX release was accomplished at pH 5.0 with release efficiency up to 60–80%. Because of the decoration of Arg-Gly-Asp (RGD) peptides onto the poly(β-amino ester)s, the DOX-encapsulated nanoparticles formed by poly(RGD-co-β-amino ester)s can be internalized by cancer cells via an αvβ3 integrin-mediated endocytosis pathway and accumulated in the lysosomes that provide an acidic environment to promote the release of DOX. Finally, the DOX-encapsulated copolymer nanoparticles with the targeted RGD peptide exhibited higher efficiency to kill U87 human glioblastoma cancer cells than that without RGD, which was further proved by cellular uptake of DOX-loaded nanoparticles.
- Published
- 2014
20. Progress in Tumor-Associated Macrophages: From Bench to Bedside
- Author
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Yi Wang, Jie Wang, Yao-Xin Lin, Sheng-Lin Qiao, and Hao Wang
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0301 basic medicine ,Stromal cell ,Angiogenesis ,medicine.medical_treatment ,Biomedical Engineering ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Biomaterials ,Mice ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Neoplasms ,Tumor Microenvironment ,Animals ,Humans ,Medicine ,skin and connective tissue diseases ,Cancer immunology ,Tumor microenvironment ,business.industry ,Macrophages ,Cancer ,Immunotherapy ,medicine.disease ,Nanomedicine ,030104 developmental biology ,Tumor progression ,030220 oncology & carcinogenesis ,Cancer research ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Tumor-associated macrophages (TAMs) are of great interest in cancer immunology as they play an important role in the tumor microenvironment as cancer stromal cells recruited from circulating monocytes. TAMs are closely associated with tumor progression, including initiation, trophic growth, metabolism, angiogenesis, and metastasis; moreover, in clinical practice, their quantity can be related to poor prognosis. Fundamental and translational studies imply that TAMs are one of the most promising targets in tumor therapy. Herein, the biological origination and classification of TAMs, which correspond to their functions and differentiations, are reviewed in detail. In addition, recent basic research and clinical preprocess of TAMs in tumor immunotherapy are also discussed. Finally, the advances in the use of nanotechnology and TAMs for tumor therapy are discussed. This review focuses on the background and status of basic research and clinical significance of TAMs, points out the potential of TAMs in tumor immunological therapy, and clarifies the possibility of translation TAM-targeting therapies in medicine.
- Published
- 2018
21. Polymeric nanoparticles promote macrophage reversal from M2 to M1 phenotypes in the tumor microenvironment
- Author
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Zeng-Ying Qiao, Hong-Wei An, Hao Wang, R. P. Yeshan J. Rajapaksha, Yang Ma, Sheng-Lin Qiao, Yao-Xin Lin, and Yi Wang
- Subjects
0301 basic medicine ,Materials science ,Polymers ,medicine.medical_treatment ,Biophysics ,Bioengineering ,02 engineering and technology ,Biomaterials ,03 medical and health sciences ,Mice ,Immune system ,Cancer immunotherapy ,Nanocapsules ,Cell Line, Tumor ,medicine ,Tumor Microenvironment ,Animals ,Cytotoxicity ,Tumor microenvironment ,Macrophages ,Immunotherapy ,Neoplasms, Experimental ,021001 nanoscience & nanotechnology ,medicine.disease ,Cellular Reprogramming ,Phenotype ,Interleukin-12 ,Mice, Inbred C57BL ,030104 developmental biology ,Mechanics of Materials ,Delayed-Action Preparations ,Immunology ,Ceramics and Composites ,Cancer research ,Systemic administration ,Female ,0210 nano-technology ,Infiltration (medical) - Abstract
Immunotherapy has shown promising treatment effects for a variety of cancers. However, the immune treatment efficiency for solid tumors is limited owing to insufficient infiltration of immune cells into solid tumors. The conversion of tumor-supportive macrophages to tumor-suppressive macrophages, inducing the functional reversal of macrophages, is an effective method and contributes to a subsequent antitumor response. The current challenge in the field is the poor distribution and systemic side effects associated with the use of cytokines. As a solution to this issue, we designed and synthesized microenvironment-responsive nanoparticles (P) with IL-12 payload (IL-12⊂P1). These nanoparticles could promote the systemic administration and release of IL-12 in the tumor microenvironment, and the locally responsive property of IL-12⊂P1 could subsequently re-educate tumor-associated macrophages (TAMs). In particular, our results illustrated the great therapeutic effects derived from the functional conversion of macrophages. Our strategy was to design a microenvironment-responsive material for local macrophage modification to overcome the physiological barrier of solid tumors. The shifting of macrophage phenotypes via IL-12⊂P1 achieved immunomodulation in the microenvironment for cancer therapy, with negligible cytotoxicity. We expect that the functional regulation of TAMs by pH-responsive nanomaterials is a promising therapeutic approach for cancer immunotherapy.
- Published
- 2016
22. Bio-orthogonally Deciphered Binary Nanoemitters for Tumor Diagnostics
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Sheng-Lin Qiao, Hong-Wei An, Li-Li Li, Hao Wang, Lei Wang, Zeng-Ying Qiao, Chao Yang, Yi Wang, and Yao-Xin Lin
- Subjects
Tumor imaging ,Diagnostic Imaging ,Materials science ,Optical property ,Nanotechnology ,Biocompatible Materials ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Biocompatible material ,01 natural sciences ,0104 chemical sciences ,Living systems ,Nanostructures ,Neoplasms ,General Materials Science ,0210 nano-technology - Abstract
Bioinspired design concept has been recognized as one of the most promising strategies for discovering new biomaterials. However, smart biomaterials that are of growing interests in biomedical field need biological processability to meet their emergent applications in vivo. Herein, a new bio-orthogonally deciphered approach has been demonstrated for modulating optical properties of nanomaterials in living systems. The self-assembled nanoemitters based on cyanine-pyrene molecule 1 with inert optical property are designed and prepared. The structure and optical feature of the nanoemitters 1 can be efficiently and reliably modulated by a unique bio-orthogonal mechanism with abundant glutathione (GSH) as an activator. As a result, the self-assembled nanoemitters 1 spontaneously exhibits binary emissions for high-performance tumor imaging in vivo. We believe that this bio-orthogonally deciphered strategy opens a new avenue for designing variable smart biomaterials or devices in biomedical applications.
- Published
- 2016
23. Thermo-Controlled in Situ Phase Transition of Polymer-Peptides on Cell Surfaces for High-Performance Proliferative Inhibition
- Author
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Lei Wang, Sheng-Lin Qiao, Hong-Wei An, Hao Wang, Yao-Xin Lin, Yang Ma, Yi Wang, and Li-Li Li
- Subjects
In situ ,Materials science ,Polymers ,Cell ,Acrylic Resins ,Molecular Conformation ,Peptide ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Phase Transition ,medicine ,General Materials Science ,chemistry.chemical_classification ,Temperature ,Polymer ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,medicine.anatomical_structure ,Biochemistry ,chemistry ,Covalent bond ,Cytoplasm ,Biophysics ,Receptor clustering ,Self-assembly ,0210 nano-technology ,Peptides - Abstract
We herein report a thermocontrolled strategy for realizing in situ conformational transition of polymer–peptide conjugates at cell surfaces to manipulate and monitor HER2 receptor clustering, which finally result in effective breast cancer cell proliferation inhibition. Functional paring motifs (HBP) are covalently linked to a synthetic thermoresponsive polymer PNIPAAm to incorporate temperature control properties to HER2 targeting peptide. At 40 °C, the PNIPAAm polymers collapse and act as a “shield” to block the aggregation of HBP. Upon cooling to 35 °C, polymers are in their extended state and HBP are expose in aqueous and aggregate subsequently with enhanced fluorescence, allowing for promoting and in situ monitoring of receptor clustering. Ultimately, HER2 receptor clustering leads to cytoplasmic domain phosphorylation, which further results in effective cancer cell proliferation inhibition. We envision that this useful approach has the potential to be applied for molecule-targeted tumor therapy.
- Published
- 2016
24. pH-Sensitive Polymeric Nanoparticles Modulate Autophagic Effect via Lysosome Impairment
- Author
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Yi Wang, Sheng-Lin Qiao, R. P. Y. J. Rajapaksha, Ruo-Xin Zhang, Lei Wang, Hao Wang, Yao-Xin Lin, Hong-Wei An, and Zeng-Ying Qiao
- Subjects
0301 basic medicine ,Programmed cell death ,Materials science ,Cell Survival ,Polymers ,Nanoparticle ,02 engineering and technology ,Biomaterials ,03 medical and health sciences ,Drug Delivery Systems ,Lysosome ,medicine ,Autophagy ,Humans ,General Materials Science ,Drug Carriers ,General Chemistry ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,Drug delivery ,MCF-7 Cells ,Surface modification ,Nanoparticles ,0210 nano-technology ,Drug carrier ,Lysosomes ,Biotechnology - Abstract
In drug delivery systems, pH-sensitive polymers are commonly used as drug carriers, and significant efforts have been devoted to the aspects of controlled delivery and release of drugs. However, few studies address the possible autophagic effects on cells. Here, for the first time, using a fluorescent autophagy-reporting cell line, this study evaluates the autophagy-induced capabilities of four types of pH-sensitive polymeric nanoparticles (NPs) with different physical properties, including size, surface modification, and pH-sensitivity. Based on experimental results, this study concludes that pH-sensitivity is one of the most important factors in autophagy induction. In addition, this study finds that variation of concentration of NPs could cause different autophagic effect, i.e., low concentration of NPs induces autophagy in an mTOR-dependent manner, but high dose of NPs leads to autophagic cell death. Identification of this tunable autophagic effect offers a novel strategy for enhancing therapeutic effect in cancer therapy through modulation of autophagy.
- Published
- 2015
25. Color Development Reaction Research of Melamine in Milk
- Author
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Zheng Xiao Liu, Ling Le Kong, Sheng Lin Qiao, and Xi Quan Sun
- Subjects
Detection limit ,Chromatography ,Chemistry ,Extraction (chemistry) ,General Engineering ,Analytical chemistry ,chemistry.chemical_element ,chemistry.chemical_compound ,Linear range ,Surface-area-to-volume ratio ,Reagent ,Chlorine ,Melamine ,Ion-exchange resin - Abstract
According to the color development reaction between starch/iodine reagent and products which obtained by fumigating melamine with chlorine, a method for determination of melamine in milk was put forward. The conditions of method were researched. The best conditions are as follows. Volume ratio of milk and extraction agent is 1:1. 5, dissolved with ultrasonic washer for 5 minutes, centrifugal separation 4000r/min for 5 minutes, extracted by C008 cation exchange resin column. The results are as follows: detection limit is 10mg/L, the linear range is 25-250 mg/L, related coefficient r is 0.994, coefficient of recovery is 94%-110%, and relatively standard deviation is 0.9%. This method is easy handling, cheap equipment, good practicability and recovery, can be used as low cost detection method.
- Published
- 2011
26. A General Strategy for Facile Synthesis and In Situ Screening of Self-Assembled Polymer-Peptide Nanomaterials
- Author
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Qiaojun Fang, Yao-Xin Lin, Sheng-Lin Qiao, Hao Wang, Di Zhang, Wenjia Lai, Chun-Yuan Hou, Yi Wang, and Zeng-Ying Qiao
- Subjects
In situ ,Models, Molecular ,Materials science ,Cell Survival ,Polymers ,Molecular Sequence Data ,Nanoparticle ,Peptide ,Nanotechnology ,02 engineering and technology ,Chemistry Techniques, Synthetic ,010402 general chemistry ,01 natural sciences ,Protein Structure, Secondary ,Nanomaterials ,In vivo ,Cell Line, Tumor ,Humans ,General Materials Science ,Amino Acid Sequence ,Peptide sequence ,chemistry.chemical_classification ,Mechanical Engineering ,Polymer ,021001 nanoscience & nanotechnology ,Combinatorial chemistry ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,Nanoparticles ,Self-assembly ,0210 nano-technology ,Peptides - Abstract
A universal strategy for efficient, mild, and purification-free synthesis and in situ screening of functional polymer-peptide nanomaterials is described. More than 1000 polymer-peptide conjugates (PPCs) with various chemical structures, compositions, and therapeutic efficacy are created. According to this strategy, the structure-function relationship of the PPCs is revealed, and the antitumor efficacies of the top performing PPCs are evaluated in vivo.
- Published
- 2015
27. pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence
- Author
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Zhong-Yu Duan, Sheng-Lin Qiao, Yu-Juan Gao, Yongmei Wang, Hao Wang, Lei Wang, Zeng-Ying Qiao, and Chun-Yuan Hou
- Subjects
In situ ,Nanostructure ,Materials science ,Polymers ,Cytological Techniques ,Supramolecular chemistry ,Bioengineering ,Nanotechnology ,macromolecular substances ,Photochemistry ,chemistry.chemical_compound ,Humans ,General Materials Science ,Electrical and Electronic Engineering ,Fluorescent Dyes ,chemistry.chemical_classification ,Aqueous solution ,Pyrenes ,Mechanical Engineering ,technology, industry, and agriculture ,General Chemistry ,Polymer ,Hydrogen-Ion Concentration ,Fluorescence ,Nanostructures ,Monomer ,chemistry ,Mechanics of Materials ,Pyrene ,HeLa Cells - Abstract
Supramolecular self-assemblies with various nanostructures in organic and aqueous solutions have been prepared with desired functions. However, in situ construction of self-assembled superstructures in physiological conditions to achieve expected biological functions remains a challenge. Here, we report a supramolecular system to realize the in situ formation of nanoaggregates in living cells. The bis(pyrene) monomers were dispersed inside of hydrophobic domains of pH-sensitive polymeric micelles and delivered to the lysosomes of cells. In the acidic lysosomes, the bis(pyrene) monomers were released and self-aggregated with turn-on fluorescence. We envision this strategy for in situ construction of supramolecular nanostructures in living cells will pave the way for molecular diagnostics in the future.
- Published
- 2015
28. Self-assembled NIR nanovesicles for long-term photoacoustic imaging in vivo
- Author
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Yi Wang, Hong-Wei An, Chun-Yuan Hou, Hao Wang, Sheng-Lin Qiao, Han-Yi Xie, Li-Li Li, Yao-Xin Lin, and Lei Wang
- Subjects
Materials science ,Light ,Supramolecular chemistry ,Analytical chemistry ,Photoacoustic imaging in biomedicine ,Contrast Media ,Nanotechnology ,Catalysis ,Self assembled ,Photoacoustic Techniques ,chemistry.chemical_compound ,Mice ,Drug Stability ,In vivo ,Neoplasms ,Materials Chemistry ,Animals ,Humans ,Cyanine ,Spectroscopy, Near-Infrared ,Molecular Structure ,Metals and Alloys ,General Chemistry ,Carbocyanines ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Radiography ,chemistry ,Ceramics and Composites ,Heterografts ,Derivative (chemistry) - Abstract
We report a supramolecular approach for preparation of photostable NIR nanovesicles based on a cyanine dye derivative as a photoacoustic (PA) contrast agent for high-performance nano-imaging.
- Published
- 2015
29. In Situ Formation of Nanofibers from Purpurin18-Peptide Conjugates and the Assembly Induced Retention Effect in Tumor Sites
- Author
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Di Zhang, Ying-Xi Zhao, Hao Wang, Sheng-Lin Qiao, Guobin Qi, and Chao Yang
- Subjects
In situ ,Materials science ,Porphyrins ,Time Factors ,Nanofibers ,Photoacoustic imaging in biomedicine ,Peptide ,Nanotechnology ,Antineoplastic Agents ,Photoacoustic Techniques ,Mice ,Microscopy, Electron, Transmission ,Cell Line, Tumor ,Animals ,Tomography, Optical ,General Materials Science ,Etoposide ,chemistry.chemical_classification ,Mechanical Engineering ,Circular Dichroism ,Temperature ,chemistry ,Mechanics of Materials ,Gelatinases ,Nanofiber ,Biophysics ,Neoplasm Transplantation ,Conjugate - Abstract
An assembly-induced retention effect for enhanced tumor photoacoustic (PA) imaging and therapeutics is described. A responsive small-molecule precursor is prepared that simultaneously self-assembles into nanofibers in tumor sites that exhibit an assembly-induced retention effect, which results in an improved PA imaging signal and enhanced therapeutic efficacy. This successful proof-of-concept study paves the way to develop novel supramolecular biomaterials for cancer diagnostics and therapeutics.
- Published
- 2015
30. Self-assembled autophagy-inducing polymeric nanoparticles for breast cancer interference in-vivo
- Author
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Hong-Wei An, Lei Wang, Zeng-Ying Qiao, Sheng-Lin Qiao, Hao Wang, Yao-Xin Lin, R. P. Yeshan J. Rajapaksha, and Yi Wang
- Subjects
Materials science ,Polymers ,Nanoparticle ,Mice, Nude ,Nanotechnology ,Antineoplastic Agents ,Breast Neoplasms ,Protein Structure, Secondary ,Self assembled ,law.invention ,Polyethylene Glycols ,Breast cancer ,Drug Delivery Systems ,In vivo ,law ,Cell Line, Tumor ,medicine ,Autophagy ,Animals ,Humans ,General Materials Science ,Cytotoxicity ,Micelles ,Protein Stability ,Mechanical Engineering ,Membrane Proteins ,Hydrogen-Ion Concentration ,medicine.disease ,Polymeric nanoparticles ,Acrylates ,Mechanics of Materials ,Cancer research ,Suppressor ,Nanoparticles ,Beclin-1 ,Apoptosis Regulatory Proteins ,Lysosomes ,Hydrophobic and Hydrophilic Interactions ,Neoplasm Transplantation - Abstract
A peptide-conjugated poly(β-amino ester) that self-assembles into micelle-like nanoparticles is prepared by a convenient and modular supramolecular approach. The polymer-beclin-1 (P-Bec1) nanoparticles display enhanced cytotoxicity to breast cancer cells through induction of autophagy. This approach overcomes two major limitations of the haploinsufficient tumor suppressor Bec1 compared to small-molecule drugs: poor delivery to tumors owing to enzymatic degradation, and unstable, non-specific bio-distribution and targeting in the tumor tissues.
- Published
- 2014
31. In Situ Manipulation of Dendritic Cells by an Autophagy-Regulative Nanoactivator Enables Effective Cancer Immunotherapy.
- Author
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Yi Wang, Yao-Xin Lin, Jie Wang, Sheng-Lin Qiao, Yu-Ying Liu, Wen-Qian Dong, Junqing Wang, Hong-Wei An, Chao Yang, Muhetaerjiang Mamuti, Lei Wang, Bo Huang, and Hao Wang
- Published
- 2019
- Full Text
- View/download PDF
32. Surface charge-conversion polymeric nanoparticles for photodynamic treatment of urinary tract bacterial infections
- Author
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Li-Li Li, Chen Shao, Yao-Xin Lin, Sheng-Lin Qiao, Hao Wang, Guobin Qi, Shijie Liu, and Zeng-Ying Qiao
- Subjects
Materials science ,Surface Properties ,medicine.medical_treatment ,Bioengineering ,Nanotechnology ,Photodynamic therapy ,Drug resistance ,medicine.disease_cause ,Microbiology ,Mice ,medicine ,Animals ,Humans ,General Materials Science ,Photosensitizer ,Electrical and Electronic Engineering ,Cytotoxicity ,chemistry.chemical_classification ,Reactive oxygen species ,Microbial Viability ,Bacteria ,biology ,Mechanical Engineering ,Pathogenic bacteria ,General Chemistry ,Antimicrobial ,biology.organism_classification ,Anti-Bacterial Agents ,RAW 264.7 Cells ,Photochemotherapy ,chemistry ,Mechanics of Materials ,Urinary Tract Infections ,Nanoparticles - Abstract
Urinary tract infections are typical bacterial infections which result in a number of economic burdens. With increasing antibiotic resistance, it is urgent that new approaches are explored that can eliminate pathogenic bacteria without inducing drug resistance. Antimicrobial photodynamic therapy (PDT) is a new promising tactic. It is a gentle in situ photochemical reaction in which a photosensitizer (PS) generates reactive oxygen species (ROS) under laser irradiation. In this work, we have demonstrated Chlorin e6 (Ce6) encapsulated charge-conversion polymeric nanoparticles (NPs) for efficiently targeting and killing pathogenic bacteria in a weakly acidic urinary tract infection environment. Owing to the surface charge conversion of NPs in an acidic environment, the NPs exhibited enhanced recognition for Gram-positive (ex. S. aureus) and Gram-negative (ex. E. coli) bacteria due to the charge interaction. Also, those NPs showed significant antibacterial efficacy in vitro with low cytotoxicity. The MIC value of NPs to E. coli is 17.91 μg ml(-1), compared with the free Ce6 value of 29.85 μg ml(-1). Finally, a mouse acute cystitis model was used to assess the photodynamic therapy effects in urinary tract infections. A significant decline (P < 0.05) in bacterial cells between NPs and free Ce6 occurred in urine after photodynamic therapy treatment. And the plated counting results revealed a remarkable bacterial cells drop (P < 0.05) in the sacrificed bladder tissue. Above all, this nanotechnology strategy opens a new door for the treatment of urinary tract infections with minimal side effects.
- Published
- 2015
33. Photoacoustic Imaging: In Situ Formation of Nanofibers from Purpurin18-Peptide Conjugates and the Assembly Induced Retention Effect in Tumor Sites (Adv. Mater. 40/2015)
- Author
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Guo Bin Qi, Sheng-Lin Qiao, Ying-Xi Zhao, Di Zhang, Chao Yang, and Hao Wang
- Subjects
In situ ,chemistry.chemical_classification ,Materials science ,chemistry ,Mechanics of Materials ,Mechanical Engineering ,Nanofiber ,Photoacoustic imaging in biomedicine ,General Materials Science ,Nanotechnology ,Peptide ,Nanomaterials ,Conjugate - Published
- 2015
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