99 results on '"Shaoru Wang"'
Search Results
2. Serratamolide is a hemolytic factor produced by Serratia marcescens.
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Robert M Q Shanks, Nicholas A Stella, Roni M Lahr, Shaoru Wang, Tara I Veverka, Regis P Kowalski, and Xinyu Liu
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Medicine ,Science - Abstract
Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.
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- 2012
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3. Observation, Analysis, and Solution: Exploring Strong Lightweight Vision Transformers via Masked Image Modeling Pre-Training.
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Jin Gao, Shubo Lin, Shaoru Wang, Yutong Kou, Zeming Li, Liang Li, Congxuan Zhang, Xiaoqin Zhang, Yizheng Wang, and Weiming Hu
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- 2024
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4. A Closer Look at Self-Supervised Lightweight Vision Transformers.
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Shaoru Wang, Jin Gao, Zeming Li, Xiaoqin Zhang 0002, and Weiming Hu
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- 2023
5. Open-Vocabulary One-Stage Detection with Hierarchical Visual-Language Knowledge Distillation.
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Zongyang Ma, Guan Luo, Jin Gao, Liang Li, Yuxin Chen, Shaoru Wang, Congxuan Zhang, and Weiming Hu
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- 2022
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6. A Simple and Strong Baseline for Universal Targeted Attacks on Siamese Visual Tracking.
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Zhenbang Li, Yaya Shi, Jin Gao, Shaoru Wang, Bing Li 0001, Pengpeng Liang, and Weiming Hu
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- 2022
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7. PDNet: Toward Better One-Stage Object Detection With Prediction Decoupling.
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Li Yang, Yan Xu, Shaoru Wang, Chunfeng Yuan, Ziqi Zhang, Bing Li 0001, and Weiming Hu
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- 2022
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8. Narrowing the Gap: Improved Detector Training With Noisy Location Annotations.
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Shaoru Wang, Jin Gao, Bing Li 0001, and Weiming Hu
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- 2022
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9. RDSNet: A New Deep Architecture forReciprocal Object Detection and Instance Segmentation.
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Shaoru Wang, Yongchao Gong, Junliang Xing, Lichao Huang, Chang Huang, and Weiming Hu
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- 2020
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10. DSPNet: Towards Slimmable Pretrained Networks based on Discriminative Self-supervised Learning.
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Shaoru Wang, Zeming Li, Jin Gao, Liang Li, and Weiming Hu
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- 2022
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11. A Closer Look at Self-supervised Lightweight Vision Transformers.
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Shaoru Wang, Jin Gao, Zeming Li, Jian Sun 0001, and Weiming Hu
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- 2022
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12. PDNet: Towards Better One-stage Object Detection with Prediction Decoupling.
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Li Yang, Yan Xu, Shaoru Wang, Chunfeng Yuan, Ziqi Zhang, Bing Li 0001, and Weiming Hu
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- 2021
13. A Simple and Strong Baseline for Universal Targeted Attacks on Siamese Visual Tracking.
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Zhenbang Li, Yaya Shi, Jin Gao, Shaoru Wang, Bing Li 0001, Pengpeng Liang, and Weiming Hu
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- 2021
14. RDSNet: A New Deep Architecture for Reciprocal Object Detection and Instance Segmentation.
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Shaoru Wang, Yongchao Gong, Junliang Xing, Lichao Huang, Chang Huang, and Weiming Hu
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- 2019
15. Biochemical Insights into the Role of Guanosine Oxidation on RNA G-Quadruplex
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Tian Tian, Hai-Yan Huang, Lai Wei, Yanyan Song, Yutong Zhang, Ling-Yu Wu, Xiang Zhou, and Shaoru Wang
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chemistry.chemical_compound ,Biochemistry ,chemistry ,Guanosine ,RNA ,heterocyclic compounds ,General Chemistry ,G-quadruplex ,DNA - Abstract
While 8-oxo-7,8-dihydro-2′-deoxyguanosine (dOG) on DNA G-quadruplex (G4) has been studied, the influence of 8-oxo-7,8-dihydro-guanosine (rOG) lesions on telomeric repeat-containing RNA (TERRA) G4 d...
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- 2020
16. Light-Driven Activation of RNA-Guided Nucleic Acid Cleavage
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Lai Wei, Wei Xiong, Tian Tian, Ping Yin, Xiang Zhou, Huimin Ji, Jiaqi Wang, Shaoru Wang, and Jian Liu
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0301 basic medicine ,Light ,Chemical biology ,01 natural sciences ,Biochemistry ,Cell Line ,03 medical and health sciences ,Genome editing ,Humans ,CRISPR ,Clustered Regularly Interspaced Short Palindromic Repeats ,Nucleic acid cleavage ,Guide RNA ,Light activation ,Gene Editing ,RNA Cleavage ,010405 organic chemistry ,Chemistry ,RNA ,Acetylation ,General Medicine ,0104 chemical sciences ,Cell biology ,030104 developmental biology ,Light driven ,Molecular Medicine ,CRISPR-Cas Systems ,RNA, Guide, Kinetoplastida - Abstract
As one of the most favorable stimuli, photoactivation provides an advantageous way to manipulate biological objects. In the current study, we have successfully demonstrated the use of light activation guide RNA (gRNA) strategy for controlling CRISPR systems. By conjugating photolabile protecting groups, the CRISPR functions became minimal, but exposure of acylated gRNAs to 365 nm light triggers the removal of masking groups, leading to the rescue of CRISPR functions. Furthermore, our strategy has been successfully used to control gene editing in human cells. This proof-of-concept study therefore demonstrates the promising potential of our strategy to versatile applications in chemical biology.
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- 2020
17. RDSNet: A New Deep Architecture forReciprocal Object Detection and Instance Segmentation
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Chang Huang, Weiming Hu, Lichao Huang, Junliang Xing, Shaoru Wang, and Yongchao Gong
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Structure (mathematical logic) ,Pixel ,Computer science ,business.industry ,020207 software engineering ,02 engineering and technology ,General Medicine ,Translation (geometry) ,Object detection ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Segmentation ,Computer vision ,Artificial intelligence ,business ,Cropping - Abstract
Object detection and instance segmentation are two fundamental computer vision tasks. They are closely correlated but their relations have not yet been fully explored in most previous work. This paper presents RDSNet, a novel deep architecture for reciprocal object detection and instance segmentation. To reciprocate these two tasks, we design a two-stream structure to learn features on both the object level (i.e., bounding boxes) and the pixel level (i.e., instance masks) jointly. Within this structure, information from the two streams is fused alternately, namely information on the object level introduces the awareness of instance and translation variance to the pixel level, and information on the pixel level refines the localization accuracy of objects on the object level in return. Specifically, a correlation module and a cropping module are proposed to yield instance masks, as well as a mask based boundary refinement module for more accurate bounding boxes. Extensive experimental analyses and comparisons on the COCO dataset demonstrate the effectiveness and efficiency of RDSNet. The source code is available at https://github.com/wangsr126/RDSNet.
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- 2020
18. ZIF-8 modified multifunctional injectable photopolymerizable GelMA hydrogel for the treatment of periodontitis
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Yun Liu, Ting Li, Maolei Sun, Zhiqiang Cheng, Wenyuan Jia, Kun Jiao, Shaoru Wang, Kongzhao Jiang, Yuheng Yang, Zhihui Dai, Liping Liu, Guomin Liu, and Yungang Luo
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Inflammation ,Biomedical Engineering ,Hydrogels ,General Medicine ,Biochemistry ,Rats ,Biomaterials ,Osteogenesis ,Zeolites ,Animals ,Gelatin ,Methacrylates ,Periodontal Pocket ,Periodontitis ,Molecular Biology ,Biotechnology - Abstract
Periodontitis is a chronic inflammatory disease caused by plaque that leads to alveolar bone resorption. In the treatment of periodontitis, it is necessary to reduce the bacterial load and promote alveolar bone regeneration. In this study, zeolitic imidazolate framework-8 (ZIF-8) is used in the treatment of periodontitis, and an injectable photopolymerizable ZIF-8/gelatin methacryloyl (GelMA) composite hydrogel (GelMA-Z) is constructed. We confirm that ZIF-8 nanoparticles are successfully loaded into GelMA, which demonstrates fluidity and photopolymerizability. GelMA-Z continuously releases Zn
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- 2021
19. A Simple and Strong Baseline for Universal Targeted Attacks on Siamese Visual Tracking
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Jin Gao, Weiming Hu, Yaya Shi, Pengpeng Liang, Bing Li, Zhenbang Li, and Shaoru Wang
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FOS: Computer and information sciences ,Network architecture ,Forcing (recursion theory) ,business.industry ,BitTorrent tracker ,Computer science ,Computer Vision and Pattern Recognition (cs.CV) ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Computer Science - Computer Vision and Pattern Recognition ,Inference ,Image (mathematics) ,Media Technology ,Trajectory ,Code (cryptography) ,Eye tracking ,Computer vision ,Artificial intelligence ,Electrical and Electronic Engineering ,business - Abstract
Siamese trackers are shown to be vulnerable to adversarial attacks recently. However, the existing attack methods craft the perturbations for each video independently, which comes at a non-negligible computational cost. In this paper, we show the existence of universal perturbations that can enable the targeted attack, e.g., forcing a tracker to follow the ground-truth trajectory with specified offsets, to be video-agnostic and free from inference in a network. Specifically, we attack a tracker by adding a universal translucent perturbation to the template image and adding a fake target, i.e., a small universal adversarial patch, into the search images adhering to the predefined trajectory, so that the tracker outputs the location and size of the fake target instead of the real target. Our approach allows perturbing a novel video to come at no additional cost except the mere addition operations – and not require gradient optimization or network inference. Experimental results on several datasets demonstrate that our approach can effectively fool the Siamese trackers in a targeted attack manner. We show that the proposed perturbations are not only universal across videos, but also generalize well across different trackers. Such perturbations are therefore doubly universal, both with respect to the data and the network architectures. Our code is available at https://github.com/lizhenbang56/SiamAttack.
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- 2021
20. Ligation-promoted hyperbranched rolling circle amplification enables ultrasensitive detection of microRNA in clinical specimens
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Xiaoe Zhang, Haitao Wang, Xi Wang, Yang Yiwen, Pei Ma, Huang Junjie, Xin Zhou, Shaoru Wang, Zhu Zirui, Yinong Liu, and Xiang Zhou
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0301 basic medicine ,Metals and Alloys ,Computational biology ,Biology ,010402 general chemistry ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomarker (cell) ,Highly sensitive ,03 medical and health sciences ,030104 developmental biology ,Rolling circle replication ,microRNA ,Materials Chemistry ,Electrical and Electronic Engineering ,Ligation ,Instrumentation - Abstract
MiRNA is reported as an important biomarker candidate and therapeutic target in a variety of diseases and cancers. However, ultrasensitive, straightforward, isothermal, low-cost and credible methods for the detection of miRNA from clinical specimens still remain challenges. Here, we establish an isothermal, highly sensitive and specific method for miRNA analysis based on a novel ligation-promoted hyperbranched rolling circle amplification (L-HRCA). This strategy exhibits 103-fold sensitivity enhancement compared to T4 ligase-assisted target-primer BRCA. The excellent specificity of L-HRCA allows for discriminating target miRNA from negative miRNAs. This method is applied to analyse the expression level of miR-21 in hepatocellular carcinoma. These results indicated that the L-HRCA strategy will become a promising tool to quantify miRNA in early clinical diagnostics.
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- 2018
21. Isoprenylcysteine carboxyl methyltransferase promotes the progression of tongue squamous cell carcinoma via the K-Ras and RhoA signaling pathways
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Fang Yang, Shaoru Wang, Jia-Wei Zheng, Jing Qiu, Qingyuan Guo, Shengchao Zhang, Zhenggang Chen, and Wei Wang
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Cell cycle checkpoint ,RHOA ,Mice, Nude ,Flow cytometry ,Mice ,Tongue ,Cell Line, Tumor ,medicine ,Animals ,Gene silencing ,Protein Methyltransferases ,General Dentistry ,Cell Proliferation ,Gene knockdown ,biology ,medicine.diagnostic_test ,Cell growth ,Chemistry ,Cell Biology ,General Medicine ,Tongue Neoplasms ,Otorhinolaryngology ,Apoptosis ,Carcinoma, Squamous Cell ,biology.protein ,Cancer research ,Signal transduction ,Signal Transduction - Abstract
Objective This research investigated the biological role of isoprenylcysteine carboxyl methyltransferase (ICMT) in tongue squamous cell carcinoma (TSCC) progression meanwhile to explore the conceivable mechanism. Methods The mRNA and protein expression were measured using real-time PCR and Western blot. Cell proliferation, apoptosis, cycle distribution, migration and invasion were evaluated by CCK-8 assay, flow cytometry, wound-healing assay and transwell assay. The anti-tumor activity of ICMT silencing was observed in nude mice. Results Our results indicated that silencing of ICMT-mediated methylation effectively inhibited TSCC cells proliferation in vitro and reduced tumor growth in vivo. Moreover, ICMT knockdown also induced cell apoptosis and cell cycle arrest of both CAL-27 and SCC-4 cells. In addition, CAL-27 and SCC-4 cells migration and invasion were weakened by ICMT siRNA. Mechanistically, ICMT deficiency significantly decreased the K-Ras and RhoA membrane targeting localization, leading to the suppression of K-Ras- and RhoA-mediated downstream signaling in CAL-27 and SCC-4 cells. Conclusions Altogether, our findings identified a crucial role played by ICMT in the progression of TSCC and the potential mechanisms by which exerted its effects, indicating that targeting ICMT may represent a promising therapeutic strategy for TSCC. Availability of data and materials The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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- 2022
22. Conditional control of RNA-guided nucleic acid cleavage and gene editing
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Tian Tian, Ping Yin, Ling-Yu Wu, Jian Liu, Lai Wei, Shaoru Wang, Wei Xiong, Xiang Zhou, and Hai-Yan Huang
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CRISPR-Cas9 genome editing ,0301 basic medicine ,Science ,Chemical biology ,General Physics and Astronomy ,Computational biology ,Biology ,010402 general chemistry ,01 natural sciences ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Genome editing ,Humans ,CRISPR ,Guide RNA ,Nucleic acid structure ,lcsh:Science ,Gene Editing ,Multidisciplinary ,Palindrome ,RNA ,General Chemistry ,Endonucleases ,0104 chemical sciences ,Nucleic acids ,030104 developmental biology ,chemistry ,lcsh:Q ,CRISPR-Cas Systems ,DNA ,HeLa Cells ,RNA, Guide, Kinetoplastida - Abstract
Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology., Constituitively active CRISPR systems have the risk of adverse off-target effects. Here the authors use chemical masking and activation of gRNA to control activity.
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- 2020
23. G-Quadruplex: A Regulator of Gene Expression and Its Chemical Targeting
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Tian Tian, Xiang Zhou, Shaoru Wang, and Yuqi Chen
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0301 basic medicine ,Regulation of gene expression ,General Chemical Engineering ,Biochemistry (medical) ,Regulator ,General Chemistry ,Computational biology ,Biology ,010402 general chemistry ,G-quadruplex ,01 natural sciences ,Biochemistry ,DNA-binding protein ,Genome ,0104 chemical sciences ,Nucleic acid secondary structure ,03 medical and health sciences ,030104 developmental biology ,Gene expression ,Materials Chemistry ,Environmental Chemistry ,Identification (biology) - Abstract
Summary G-quadruplex (G4) is an important type of nucleic acid secondary structure. An abundance of potential G4-forming sites have been shown to exist in genomes, leading to increasing interest in this research field. G4 motifs are thought to be involved in the regulation of diverse biological processes and to interact with various protein factors. Because of their important regulatory functions, G4s could have a variety of applications, the most meaningful of which is the role of G4s as potential targets of antitumor therapies. Here, we focus on the regulatory functions of G4s in tumor-related gene regulation and the use of G4s in the design of antitumor therapies, including relatively recently reported G4-related binding proteins, regulatory mechanisms, and G4-ligand designs. We also introduce G4 probes for the identification of G4 structure formation in live cells. Finally, we describe some challenges in this field and the new G4-related research field.
- Published
- 2018
24. A highly efficient fluorescence-based switch-on detection method of 5-formyluracil in DNA
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Zhiyong He, Wei Yang, Jiaqi Wang, Xiang Zhou, Yi Chen, Guangrong Zou, Shaoru Wang, Yafen Wang, Yuqi Chen, Chaoxing Liu, Fan Wu, and Xiong Zhang
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0301 basic medicine ,Phosphoramidite ,Condensed Matter Physics ,Fluorescence ,Atomic and Molecular Physics, and Optics ,03 medical and health sciences ,chemistry.chemical_compound ,genomic DNA ,030104 developmental biology ,chemistry ,Biochemistry ,Nucleic acid ,General Materials Science ,Epigenetics ,Electrical and Electronic Engineering ,Thymidine ,DNA ,Cytosine - Abstract
The identification of hydroxylmethyl- and formylpyrimidines in genomic DNA was a landmark event in epigenetics. Numerous laboratories in related fields are investigating the biology of these and other nucleic acid modifications. However, limitations in the ability to detect and synthesize appropriate modifications are an impediment. Herein, we explored a remarkable development in the selective detection of 5-formyluracil in both single-stranded and double-stranded DNA under mild conditions. The “switch-on” specificity towards 5-formyluracil enabled a high signal-to-noise ratio in qualitatively and quantitatively detecting materials containing 5-formyluracil, which is not affected by the presence of abasic sites and 5-formylcytosine, the modified cytosine counterpart of 5-formyluracil. In summary, the innoxiousness, convenience, and cost-efficiency of the 5-formyluracil phosphoramidite synthetic routine would promote the understanding of the epigenetic role of this natural thymidine modification.
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- 2017
25. Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA
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Zonggui Chen, Xiaocheng Weng, Weiwu Zeng, Yafen Wang, Wei Yang, Yu Zhou, Shaoru Wang, Fan Wu, Jinguo Huang, Zhiguo Wu, Xiong Zhang, Guangrong Zou, Xiang Zhou, and Chaoxing Liu
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0301 basic medicine ,Resolution (mass spectrometry) ,biology ,Oligonucleotide ,Base pair ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Combinatorial chemistry ,Fluorescence ,0104 chemical sciences ,Nucleobase ,Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Biochemistry ,biology.protein ,Illumina dye sequencing ,Polymerase ,DNA - Abstract
Simultaneous fluorogenic switch-on detection and single-base resolution analysis of 5fC through yielding an intramolecular cyclization nucleobase has been presented., 5-Formylcytosine (5fC), which plays an important role in epigenetic functions, has received widespread attention in many related fields. Here, we demonstrate a new design for both the fluorogenic switch-on detection and single-base resolution analysis of 5fC through selectively reacting a reagent with 5fC to yield an intramolecular cyclization nucleobase. The generated product, bearing a similar benzothiazole-iminocoumarin scaffold, is highly fluorescent and enables us to qualitatively and quantitatively detect 5fC moieties in γ-irradiated calf thymus DNA. Additionally, losing the exocyclic 4-amino group in 5fC causes the incorporation of dATP through base pairing with the generated nucleobase during polymerase extension, which helped us to analyze the 5fC sites in both single- and double-stranded oligonucleotides. Our Sanger and Illumina sequencing results show great potential in single-base resolution analysis of 5fC. It is hopeful that a similar design may be used for more detection targets.
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- 2017
26. Reversible manipulation of the G-quadruplex structures and enzymatic reactions through supramolecular host–guest interactions
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Tian Tian, Si-Min Liu, Shaoru Wang, Boshi Fu, Zhiyong He, Yanyan Song, Fan Wu, Lai Wei, Xi-Ran Yang, Conggang Li, Guohua Xu, Xiang Zhou, and Jiaqi Wang
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Bridged-Ring Compounds ,Future studies ,Supramolecular chemistry ,Chemical biology ,macromolecular substances ,Biology ,010402 general chemistry ,G-quadruplex ,01 natural sciences ,Molecular Docking Simulation ,Enzyme catalysis ,Chemical Biology and Nucleic Acid Chemistry ,Piperidines ,Genetics ,Humans ,Base Sequence ,010405 organic chemistry ,Intermolecular force ,technology, industry, and agriculture ,Imidazoles ,Thrombin ,DNA ,Telomere ,Combinatorial chemistry ,0104 chemical sciences ,G-Quadruplexes ,Biochemistry ,Nucleic acid ,Azo Compounds - Abstract
Supramolecular chemistry addresses intermolecular forces and consequently promises great flexibility and precision. Biological systems are often the inspirations for supramolecular research. The G-quadruplex (G4) belongs to one of the most important secondary structures in nucleic acids. Until recently, the supramolecular manipulation of the G4 has not been reported. The present study is the first to disclose a supramolecular switch for the reversible control of human telomere G4s. Moreover, this supramolecular switch has been successfully used to manipulate an enzymatic reaction. Using various methods, we show that cucurbit[7]uril preferably locks and encapsulates the positively charged piperidines of Razo through supramolecular interactions. They can switch the conformations of the DNA inhibitor between a flexible state and the rigid G4 and are therefore responsible for the reversible control of the thrombin activity. Thus, our findings open a promising route and exhibit potential applications in future studies of chemical biology.
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- 2017
27. The m6A methylation perturbs the Hoogsteen pairing-guided incorporation of an oxidized nucleotide
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Lai Wei, Yafen Wang, Tian Tian, Jiaqi Wang, Yanyan Song, Xin Li, Boshi Fu, Yinong Liu, Xiang Zhou, and Shaoru Wang
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0301 basic medicine ,chemistry.chemical_classification ,biology ,Stereochemistry ,Base pair ,Chemistry ,DNA polymerase ,General Chemistry ,Reverse transcriptase ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Deoxyribose ,Nucleic acid ,biology.protein ,heterocyclic compounds ,A-DNA ,Nucleotide ,DNA - Abstract
Natural nucleic acid bases can form Watson–Crick (WC) or Hoogsteen (HG) base pairs. Importantly, 8-oxo-2′-deoxyguanosine (8-oxo-dG) in DNA or 8-oxo-dG 5′-triphosphate (8-oxo-dGTP) favors a syn conformation because of the steric repulsion between O8 and O4′ of the deoxyribose ring. 8-oxo-dGTP can be incorporated into DNA opposite the templating adenine (A) using HG pairing as the dominant mechanism. Both RNA and DNA can be methylated at the N6 position of A to form N6-methyladenine (m6A). It has been found that certain viral infections may trigger an increase in the production of both 8-oxo-dGTP and m6A. The current study aims to systematically explore the effects of m6A methylation on HG base pairs and the consequent nucleotide incorporation. Our thermodynamic melting study shows that the m6A·8-oxo-dG is significantly less stable than the A·8-oxo-dG base pair in the paired region of a DNA duplex. Moreover, we have used pre-steady-state kinetics to examine the incorporation of 8-oxo-dGTP opposite m6A relative to A by a variety of reverse transcriptase (RT) enzymes and DNA polymerase (DNA pol) enzymes such as the human immunodeficiency virus type 1 (HIV-1) RT and human DNA pol β. The results demonstrate that all of these enzymes incorporate 8-oxo-dGTP less efficiently opposite m6A relative to A. Considering the steric bulk of the purine–purine pair between 8-oxo-dG and A, m6A methylation may affect the HG pairing to a great extent. Hence, it will be unfavorable to incorporate 8-oxo-dGTP into the growing strand opposite m6A. Moreover, the impeded incorporation of 8-oxo-dGTP opposite m6A has been extended to determine m6A at pre-defined positions in human rRNA. Our study may provide new insights into the roles of m6A in reducing the mutagenic potential of cellular 8-oxo-dGTP.
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- 2017
28. Enrichment and fluorogenic labelling of 5-formyluracil in DNA
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Chaoxing Liu, Xiang Zhou, Wei Yang, Yuqi Chen, Qian Yao, Yafen Wang, Guangrong Zou, Jiaqi Wang, Xiong Zhang, Fan Wu, and Shaoru Wang
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biology ,010405 organic chemistry ,5-formyluracil ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Thymine ,Chemistry ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Biotin ,Labelling ,Biotinylation ,biology.protein ,DNA ,Polymerase - Abstract
Biotinylated o-phenylenediamine directly tethered to naphthalimide can both enrich and fluorogenically label 5-formyluracil in DNA under physiological conditions., Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.
- Published
- 2017
29. Epigenetic modification of nucleic acids: from basic studies to medical applications
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Tian Tian, Tingting Hong, Xiang Zhou, Yuqi Chen, Shaoru Wang, and Jing Mo
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Epigenomics ,0301 basic medicine ,Regulation of gene expression ,RNA ,DNA ,General Chemistry ,Computational biology ,Disease ,Biology ,medicine.disease_cause ,Epigenesis, Genetic ,03 medical and health sciences ,030104 developmental biology ,Biochemistry ,Heredity ,Nucleic acid ,medicine ,Humans ,Epigenetics ,Clinical Medicine ,Epigenesis - Abstract
The epigenetic modification of nucleic acids represents one of the most significant areas of study in the field of nucleic acids because it makes gene regulation more complex and heredity more complicated, thus indicating its profound impact on aspects of heredity, growth, and diseases. The recent characterization of epigenetic modifications of DNA and RNA using chemical labelling strategies has promoted the discovery of these modifications, and the newly developed single-base or single-cell resolution mapping strategies have enabled large-scale epigenetic studies in eukaryotes. Due to these technological breakthroughs, several new epigenetic marks have been discovered that have greatly extended the scope and impact of epigenetic modifications in nucleic acids over the past few years. Because epigenetics is reversible and susceptible to environmental factors, it could potentially be a promising direction for clinical medicine research. In this review, we have comprehensively discussed how these epigenetic marks are involved in disease, including the pathogenesis, prevention, diagnosis and treatment of disease. These findings have revealed that the epigenetic modification of nucleic acids has considerable significance in various areas from methodology to clinical medicine and even in biomedical applications.
- Published
- 2017
30. Correction to 'Application of the N-Halogeno-N-sodiobenzenesulfonamide Reagents to the Selective Detection of 5-Methylcytosine in DNA Sequences'
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Xiaolong Zheng, Wuxiang Mao, Xiang Zhou, Xiwen Xing, Xiaocheng Weng, Tian Tian, Jinjun Wu, Bi-Feng Yuan, Tun Tang, Liang Xu, Shaoru Wang, Qianqian Zhai, Lin Zhuang, Bing Huang, Tingting Hong, and Tianlu Wang
- Subjects
5-Methylcytosine ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Stereochemistry ,Reagent ,General Chemistry ,Biochemistry ,Catalysis ,DNA sequencing - Published
- 2019
31. Chemical Targeting of a G-Quadruplex RNA in the Ebola Virus L Gene
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Peng Gong, Tian Tian, Guohua Xu, Bo Zhang, Yafen Wang, Xiao-Dan Li, Qiu-Yan Zhang, Xing-Yi Ge, Shaoru Wang, Xiang Zhou, Yanyan Song, Jiaqi Wang, Boshi Fu, and Bo Shu
- Subjects
Gene Expression Regulation, Viral ,0301 basic medicine ,Zaire ebolavirus ,Porphyrins ,Clinical Biochemistry ,RNA-dependent RNA polymerase ,Virus Replication ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,VP40 ,Drug Discovery ,Gene expression ,medicine ,heterocyclic compounds ,Molecular Biology ,Pharmacology ,Ebola virus ,biology ,RNA ,RNA virus ,Ebolavirus ,biology.organism_classification ,Virology ,Molecular biology ,0104 chemical sciences ,G-Quadruplexes ,RNA silencing ,030104 developmental biology ,RNA, Viral ,Molecular Medicine - Abstract
In the present study, our bioinformatics analysis first reveals the existence of a conserved guanine-rich sequence within the Zaire ebolavirus L gene. Using various methods, we show that this sequence tends to fold into G-quadruplex RNA. TMPyP4 treatment evidently inhibits L gene expression at the RNA level. Moreover, the mini-replicon assay demonstrates that TMPyP4 effectively inhibits the artificial Zaire ebolavirus mini-genome and is a more potent inhibitor than ribavirin. Although TMPyP4 treatment reduced the replication of the mutant mini-genome when G-quadruplex formation was abolished in the L gene, its inhibitory effect was significantly alleviated compared with wild-type. Our findings thus provide the first evidence that G-quadruplex RNA is present in a negative-sense RNA virus. Finally, G-quadruplex RNA stabilization may represent a new therapeutic strategy against Ebola virus disease.
- Published
- 2016
32. A novel resorufin based fluorescent 'turn-on' probe for the selective detection of hydrazine and application in living cells
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Jiaqi Wang, Zhiyong He, Tun Tang, Xiang Zhou, Boshi Fu, Shaoru Wang, Heng Xiao, Fan Wu, and Yuqi Chen
- Subjects
Detection limit ,010405 organic chemistry ,Stereochemistry ,Metal ions in aqueous solution ,Hydrazine ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,High-performance liquid chromatography ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Naked eye ,Selectivity ,Derivative (chemistry) ,Nuclear chemistry - Abstract
In this study, a resorufin derivative RTP-1, which is a novel fluorescent “turn-on” probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 μmol/L, with a detection limit of 0.84 μmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory (DFT). Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine (100 μmol/L) for 30 min.
- Published
- 2016
33. N6-Methyladenine hinders RNA- and DNA-directed DNA synthesis: application in human rRNA methylation analysis of clinical specimens
- Author
-
Yanyan Song, Xiang Zhou, Tian Tian, Kai Gu, Jiaqi Wang, Xiaoe Zhang, Xin Zhou, Pei Ma, Boshi Fu, Shaoru Wang, and Xiao-Lian Zhang
- Subjects
0301 basic medicine ,DNA clamp ,biology ,DNA polymerase ,Base pair ,DNA polymerase II ,RRNA methylation ,General Chemistry ,Molecular biology ,03 medical and health sciences ,030104 developmental biology ,biology.protein ,Primase ,Primer (molecular biology) ,Polymerase - Abstract
N 6-Methyladenine (m6A) is the most abundant internal modification on mammalian mRNA. Very recently, m6A has been reported as a potentially important ‘epigenetic’ mark in eukaryotes. Until now, site-specific detection of m6A is technically very challenging. Here, we first reveal that m6A significantly hinders DNA- and RNA-directed DNA synthesis. Systematic investigations of 5′-triphosphates of a variety of 5-substituted 2′-deoxyuridine analogs in primer extension have been performed. In the current study, a quantitative analysis of m6A in the RNA or DNA context has been achieved, using Bst DNA polymerase catalyzed primer extension. Molecular dynamics study predicted that m6A in template tends to enter into and be restrained in the MGR region of Bst DNA polymerase, reducing conformational flexibility of the DNA backbone. More importantly, a site-specific determination of m6A in human ribosomal RNA (rRNA) with high accuracy has been afforded. Through a cumulative analysis of methylation alterations, we first reveal that significantly cancer-related changes in human rRNA methylation were present in patients with hepatocellular carcinoma.
- Published
- 2016
34. Right-handed and left-handed G-quadruplexes have the same DNA sequence: distinct conformations induced by an organic small molecule and potassium
- Author
-
Tianrui Xue, Xiang Zhou, Jinguo Huang, Boshi Fu, Shaoru Wang, Yafen Wang, Yuqi Chen, and Guohua Xu
- Subjects
0301 basic medicine ,Circular dichroism ,Stereochemistry ,Sequence (biology) ,010402 general chemistry ,G-quadruplex ,01 natural sciences ,Catalysis ,Small Molecule Libraries ,03 medical and health sciences ,chemistry.chemical_compound ,Materials Chemistry ,heterocyclic compounds ,Oligonucleotide ,Chemistry ,Circular Dichroism ,Metals and Alloys ,DNA ,General Chemistry ,Small molecule ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,G-Quadruplexes ,Kinetics ,030104 developmental biology ,Potassium ,Ceramics and Composites ,Nucleic acid ,Thermodynamics - Abstract
Herein, we report two distinct G-quadruplex conformations of the same G-rich oligonucleotide, regulated by a small molecule. This is the first report in which both right- and left-handed G-quadruplex conformations have been obtained from the same sequence. We discriminated these two distinct conformations and investigated their kinetics and thermodynamics.
- Published
- 2016
35. Convenient and multiplexed detection of microRNAs based on an exonucleation reaction by conformational switch of hairpin probes
- Author
-
Xin Zhou, Xiaoe Zhang, Yafen Wang, Jiaqi Wang, Zhizhi Kong, Xiang Zhou, and Shaoru Wang
- Subjects
0301 basic medicine ,biology ,DNA polymerase ,Chemistry ,Hybridization probe ,Metals and Alloys ,RNA ,Computational biology ,Condensed Matter Physics ,Molecular biology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Molecular recognition ,Duplex (building) ,microRNA ,Materials Chemistry ,biology.protein ,A-DNA ,Electrical and Electronic Engineering ,Instrumentation ,DNA - Abstract
The ability to detect and quantify microRNA (miRNA) could greatly facilitate the comprehension of miRNA-mediated biological process and related disease in more detail. We developed, for the first time, a convenient and efficient strategy for miRNA detection based on direct molecular recognition between initially quenched DNA probes and miRNA targets. The loop region of dual labeled DNA probe with hairpin shape was complementary to target miRNA, which could induce a corresponding conformational switch, a DNA:RNA hybrid duplex was formed with a single-strand DNA overhang, which could be recognized and degraded by various exonucleases, including Exonuclease I (Exo I) and T4 DNA polymerase. Fluorescence enhancement was generated by the degradation, which occurred in the 3′–5′ direction. This method represented a reliable way to distinguish different miRNAs with high homology, such as different members of the miR-200 family. Furthermore, multiplexed miRNAs detection could be achieved with our developed approach. More importantly, this proposed method can distinguish the expression level of miR-21 in human liver tissues between liver cancer tissues and normal tissues. This exonucleation-based fluorescence enhancement strategy holds great promise for further applications in early miRNA-related disease diagnosis and prognosis.
- Published
- 2016
36. CD and NMR investigation of collagen peptides mimicking a pathological Gly-Ser mutation and a natural interruption in a similar highly charged sequence context
- Author
-
Songqing Liu, Shaoru Wang, Xiuxia Sun, Jianxi Xiao, and Wenyuan Yu
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Circular dichroism ,Hydrogen bond ,Stereochemistry ,Point mutation ,Peptide ,Biology ,Biochemistry ,Serine ,03 medical and health sciences ,030104 developmental biology ,chemistry ,Biophysics ,Protein folding ,Molecular Biology ,Peptide sequence ,Triple helix - Abstract
Even a single Gly substitution in the triple helix domain of collagen leads to pathological conditions while natural interruptions are suggested to play important functional roles. Two peptides-one mimicking a pathological Gly-Ser substitution (ERSEQ) and the other one modeling a similar natural interruption sequence (DRSER)-are designed to facilitate the comparison for elucidating the molecular basis of their different biological roles. CD and NMR investigation of peptide ERSEQ indicates a reduction of the thermal stability and disruption of hydrogen bonding at the Ser mutation site, providing a structural basis of the OI disease resulting from the Gly-Ser mutation in the highly charged RGE environment. Both CD and NMR real-time folding results indicate that peptide ERSEQ displays a comparatively slower folding rate than peptide DRSER, suggesting that the Gly-Ser mutation may lead to a larger interference in folding than the natural interruption in a similar RSE context. Our studies suggest that unlike the rigid GPO environment, the abundant R(K)GE(D) motif may provide a more flexible sequence environment that better accommodates mutations as well as interruptions, while the electrostatic interactions contribute to its stability. These results shed insight into the molecular features of the highly charged motif and may aid the design of collagen biomimetic peptides containing important biological sites.
- Published
- 2015
37. The Manipulation of RNA‐Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
- Author
-
Lai Wei, Ping Yin, Tian Tian, Hai-Yan Huang, Shaoru Wang, Jian Liu, Wei Xiong, Ling-Yu Wu, and Xiang Zhou
- Subjects
ninhydrin chemistry ,General Chemical Engineering ,Chemical biology ,General Physics and Astronomy ,Medicine (miscellaneous) ,postsynthetic modification ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,chemistry.chemical_compound ,Moiety ,CRISPR ,General Materials Science ,Guide RNA ,lcsh:Science ,Nuclease ,Full Paper ,biology ,General Engineering ,RNA ,Protein engineering ,Full Papers ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,Biochemistry ,Ninhydrin ,biology.protein ,lcsh:Q ,0210 nano-technology - Abstract
CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome‐editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need for protein engineering. The CRISPR systems usually require a guide RNA (gRNA) moiety to recruit and direct the nuclease complexes. In this respect, the ninhydrin (1,2,3‐indantrione monohydrate) seems to have considerable potential, as yet unexploited, for modifying gRNA. In this study, ninhydrin chemistry is explored for reversible postsynthetic modification of gRNA molecules. It is further shown that ninhydrin chemistry is efficient in modulating two important CRISPR systems. Thus, ninhydrin chemistry exhibits potential applications in future chemical biology studies., In this study, ninhydrin chemistry is explored for reversible modification of guide RNA molecules. It is further shown that ninhydrin chemistry is useful in modulating two important clustered regularly interspaced short palindromic repeats systems. Thus, ninhydrin chemistry exhibits potential applications in future chemical biology studies.
- Published
- 2020
38. The Cucurbit[7]Uril‐Based Supramolecular Chemistry for Reversible B/Z‐DNA Transition
- Author
-
Ling-Yu Wu, Boshi Fu, Lai Wei, Shaoru Wang, Conggang Li, Xi-Ran Yang, Guohua Xu, Xiang Zhou, Yanyan Song, Simin Liu, and Jiaqi Wang
- Subjects
cucurbit[7]uril ,Conformational change ,spermine ,Stereochemistry ,General Chemical Engineering ,Supramolecular chemistry ,General Physics and Astronomy ,Medicine (miscellaneous) ,Spermine ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,supramolecular chemistry ,Z-DNA ,chemistry.chemical_compound ,Transcription (biology) ,Moiety ,General Materials Science ,Full Paper ,010405 organic chemistry ,General Engineering ,Biological activity ,Full Papers ,0104 chemical sciences ,chemistry ,reversible B/Z‐DNA transitions ,DNA - Abstract
As a left‐handed helical structure, Z‐DNA is biologically active and it may be correlated with transcription and genome stability. Until recently, it remained a significant challenge to control the B/Z‐DNA transition under physiological conditions. The current study represents the first to reversibly control B/Z‐DNA transition using cucurbit[7]uril‐based supramolecular approach. It is demonstrated that cucurbit[7]uril can encapsulate the central butanediamine moiety [HN(CH2)4NH] and reverses Z‐DNA caused by spermine back to B‐DNA. The subsequent treatment with 1‐adamantanamine disassembles the cucurbit[7]uril/spermine complex and readily induces reconversion of B‐ into Z‐DNA. The DNA conformational change is unequivocally demonstrated using different independent methods. Direct evidence for supramolecular interactions involved in DNA conformational changes is further provided. These findings can therefore open a new route to control DNA helical structure in a reversible way.
- Published
- 2018
39. A two-photon fluorescent probe for selective methylglyoxal detection and application in living cells
- Author
-
Tun Tang, Yan Feng, Shaoru Wang, Xiang Zhou, Yimin Zhou, Yuqi Chen, Mengjun Li, and Changcheng Wang
- Subjects
Detection limit ,General Chemical Engineering ,Methylglyoxal ,General Engineering ,Photochemistry ,Mass spectrometry ,Fluorescence ,Peroxide ,Photoinduced electron transfer ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Two-photon excitation microscopy ,Desorption - Abstract
Methods based on fluorescent “turn-on” sensors have been developed for methylglyoxal (MGO) detection, but the two-photon one is hardly ever reported. In this study, a naphthalimide derivative PDN-1, which is a novel two-photon fluorescent “turn-on” probe for rapid and sensitive detection of methylglyoxal, is designed and synthesized. Under physiological conditions, the probe showed minimal background emission, whereas, after interacting with MGO, it exhibited significant fluorescence enhancement (33-fold) through the inhibition of the photoinduced electron transfer (PET) effect. The fluorescence enhancement showed a decent linear relationship with the MGO concentration ranging from 0 to 10 μM, with a detection limit of 77 nM. The mechanism of the PDN-1–MGO reaction was evaluated by 1H, 13C NMR spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The specificity of PDN-1 for MGO to other aldehydes, ions, peroxide and nitric oxide is satisfactory. Imaging MGO in living cells by PDN-1 is also accomplished with high sensitivity and low cytotoxicity.
- Published
- 2015
40. Novel Amplex Red Oxidases Based on Noncanonical DNA Structures: Property Studies and Applications in MicroRNA Detection
- Author
-
Boshi Fu, Shuang Peng, Tian Tian, Yuelin Long, Pu Guo, Shaoru Wang, Xiaoe Zhang, Jiaqi Wang, and Xiang Zhou
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Circular dichroism ,Guanine ,Circular Dichroism ,Deoxyribozyme ,Biosensing Techniques ,DNA ,Oligomer ,Fluorescence ,Analytical Chemistry ,MicroRNAs ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Nucleic Acid Conformation ,Spectrophotometry, Ultraviolet ,Oxidoreductases ,Protein secondary structure ,Hemin - Abstract
G-triplex has recently been identified as a new secondary structure in G-rich sequences. However, its functions and biological roles remain largely unknown. This study first developed two kinds of Amplex Red oxidases, which were based on relatively new G-triplex structure and a common G-quadruplex one. A collection of DNA binding assays including circular dichroism (CD) spectroscopy, a CD melting assay, and a UV titration study were used to determine the G-triplex structure of G3 oligomer. The low intrinsic oxidative activity of hemin was significantly enhanced using G-triplex or G-quadruplex. Only one key guanine deletion from the G3 oligomer or G4 one could result in a much decreased Amplex Red oxidation activity. To the best of our knowledge, this is the first case reporting direct use of air as the oxidant for fluorescence generation based on DNAzyme strategies. Further mechanism studies demonstrated an involvement of on-site H2O2 generation from O2 and water and a following oxidation of Amplex Red to resorufin, causing a fluorescence enhancement. Furthermore, the newly developed oxidases have been effectively used in microRNA detection, using only one biotin-labeled probe and one small-molecule substrate. The conjugation of a target DNA to the G-triplex- or G-quadruplex-forming sequence enabled one to produce G-triplex or G-quadruplex by endonuclease in the presence of a slight amount of miRNA and amplify the signal of fluorescence from the oxidation of Amplex Red. Our findings of novel Amplex Red oxidases could potentially be used in a wide range of applications.
- Published
- 2014
41. Correction: Corrigendum: Existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy
- Author
-
Yuqi Chen, Xiang Zhou, Tian Tian, Xiwen Xing, Shaoru Wang, Liang Xu, Qianqian Zhai, Zhengan Zhang, Xiaocheng Weng, Bi-Feng Yuan, Yu-Qi Feng, Shengyong Yan, and Libo Yuan
- Subjects
Models, Molecular ,0301 basic medicine ,Crosslinking of DNA ,Computational biology ,Phenylenediamines ,G-quadruplex ,Article ,Polyethylene Glycols ,Mice ,Xenopus laevis ,03 medical and health sciences ,Tumor Cells, Cultured ,Animals ,Ficoll ,Humans ,heterocyclic compounds ,Promoter Regions, Genetic ,Melanoma ,Schiff Bases ,Cell Proliferation ,Multidisciplinary ,Molecular Structure ,Chemistry ,Circular Dichroism ,Promoter ,DNA ,Oncogenes ,Corrigenda ,G-Quadruplexes ,Cross-Linking Reagents ,030104 developmental biology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Oocytes ,Female ,Comet Assay ,DNA Damage ,HeLa Cells - Abstract
Existence of G-quadruplex DNA in vivo always attract widespread interest in the field of biology and biological chemistry. We reported our findings for the existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy. Probes for selective G-quadruplex cross-linking was designed and synthesized that show high selectivity for G-quadruplex cross-linking. Further biological studies demonstrated its good inhibition activity against murine melanoma cells. To further investigate if G-quadruplex DNA was formed in vivo and as the target, a derivative was synthesized and pull-down process toward chromosome DNAs combined with circular dichroism and high throughput deep sequencing were performed. Several simulated intracellular conditions, including X. laevis oocytes, Ficoll 70 and PEG, was used to investigate the compound's pure cross-linking ability upon preformed G-quadruplex. Thus, as a potent G-quadruplex cross-linking agent, our strategy provided both valuable evidence of G-quadruplex structures in vivo and intense potential in anti-cancer therapy.
- Published
- 2016
42. In honor of Professor Li-He Zhang on the occasion of his 80th birthday
- Author
-
Shaoru Wang and Xinjing Tang
- Subjects
Pharmacology ,Portrait ,History of pharmacy ,Honor ,media_common.quotation_subject ,Drug Discovery ,Zhàng ,Molecular Medicine ,Historical Article ,Biography ,Art ,Classics ,media_common - Published
- 2018
43. Systematic Investigations of Different Cytosine Modifications on CpG Dinucleotide Sequences: The Effects on the B-Z Transition
- Author
-
Yuelin Long, Tian Tian, Jiaqi Wang, Xiang Zhou, Shaoru Wang, Yu-Shu Ge, Pu Guo, and Yi Liu
- Subjects
Epigenomics ,Circular dichroism ,Molecular Structure ,Transition (genetics) ,Chemistry ,Circular Dichroism ,Oxidation reduction ,DNA ,General Chemistry ,Biochemistry ,Catalysis ,Cytosine ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,CpG site ,Humans ,Epigenetics ,Oxidation-Reduction ,Dinucleoside Phosphates - Abstract
We have first demonstrated the distinctive effects of three newly reported epigenetic modifications, including 5hmC, 5fC, and 5caC, on B-Z transition of CpG dinucleotide DNAs. We have performed detailed assays and compared their effects. We further studied the regulation of B-Z transition of CpG dinucleotide dodecamers by alternating oxidation and alternating reduction.
- Published
- 2013
44. Application of N-Halogeno-N-sodiobenzenesulfonamide Reagents to the Selective Detection of 5-Methylcytosine in DNA Sequences
- Author
-
Tingting Hong, Tun Tang, Tianlu Wang, Xiwen Xing, Xiaolong Zheng, Liang Xu, Tian Tian, Xiaocheng Weng, Qianqian Zhai, Shaoru Wang, Xiang Zhou, Bing Huang, Wuxiang Mao, Jinjun Wu, Lin Zhuang, and Bi-Feng Yuan
- Subjects
Sulfonamides ,Base Sequence ,Molecular Structure ,Temperature ,Locus (genetics) ,DNA ,General Chemistry ,Hydrogen-Ion Concentration ,Nitro Compounds ,Biochemistry ,Catalysis ,DNA sequencing ,5-Methylcytosine ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Reagent ,Moiety ,Deoxycytidine - Abstract
To surmount the challenges of the locus determination and accurate quantification of 5-methyl-2'-deoxycytidine ((5Me)dC) in DNA fragments that contain multiple (5Me)dC residues, we designed and synthesized two N-halogeno-N-sodiobenzenesulfonamide reagents that provide a new chemical method for probing (5Me)dC in DNA sequences. When the strategy we provided was combined with β-glucosyltransferase, (5Me)dC could be distinguished from 5-hydroxymethyl-2'-deoxycytidine ((5hm)dC) and deoxycytidine (dC) through the introduction of a glucose moiety to the hydroxyl group of (5hm)dC.
- Published
- 2013
45. Recent progress of nucleic acid probes based on small molecules
- Author
-
Tian Tian, Zhou Xiang, Weng Xiaocheng, Shaoru Wang, and Heng Xiao
- Subjects
chemistry.chemical_classification ,General Chemical Engineering ,General Chemistry ,Conjugated system ,G-quadruplex ,Biochemistry ,Small molecule ,Combinatorial chemistry ,Cyclic peptide ,Z-DNA ,chemistry.chemical_compound ,chemistry ,Helix ,Materials Chemistry ,Nucleic acid ,DNA - Abstract
This paper reviews the latest research progress of the nucleic acid probes based on small molecules. The related nucleic acid structures include quadruplex nucleic acids (including G-quadruplex and i-motif), triple-stranded nucleic acid structures, left-handed DNA helix structures, as well as irregular nucleic acid structures (bulge structures and loop structures). The related small molecules includ transition metal complexes, the macrocyclic conjugated compounds, cyclic peptides, and oligosaccharide antibiotics.
- Published
- 2012
46. A highly conserved G-rich consensus sequence in hepatitis C virus core gene represents a new anti–hepatitis C target
- Author
-
Tian Tian, Yanyan Song, Ying Zhu, Zhiguo Wu, Yuqi Chen, Ling-Yu Wu, Boshi Fu, Nan-Fang Peng, Gai Huang, Zhi-Xian Qiao, Rong Huang, Wuxiang Mao, Guohua Xu, Xiang Zhou, Fan Wu, Xiao-Lian Zhang, Shaoru Wang, Shuang Peng, Yuan-Qin Min, Jiaqi Wang, and Chaoxing Liu
- Subjects
0301 basic medicine ,Hepatitis C virus ,Genome, Viral ,Hepacivirus ,Computational biology ,Biology ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Small Molecule Libraries ,03 medical and health sciences ,Chemical Biology ,medicine ,Consensus sequence ,Humans ,Gene ,Conserved Sequence ,Research Articles ,Hepatitis ,guanine-rich consensus sequence ,targeting G-quadruplex RNA ,Multidisciplinary ,Viral Core Proteins ,SciAdv r-articles ,RNA ,Genetic Therapy ,Hepatitis C ,medicine.disease ,Virology ,0104 chemical sciences ,G-Quadruplexes ,NS2-3 protease ,030104 developmental biology ,Nucleic acid ,Nucleic Acid Conformation ,RNA, Viral ,Research Article - Abstract
A conserved guanine-rich sequence could be a new target for anti–hepatitis C virus drug development., G-quadruplex (G4) is one of the most important secondary structures in nucleic acids. Until recently, G4 RNAs have not been reported in any ribovirus, such as the hepatitis C virus. Our bioinformatics analysis reveals highly conserved guanine-rich consensus sequences within the core gene of hepatitis C despite the high genetic variability of this ribovirus; we further show using various methods that such consensus sequences can fold into unimolecular G4 RNA structures, both in vitro and under physiological conditions. Furthermore, we provide direct evidences that small molecules specifically targeting G4 can stabilize this structure to reduce RNA replication and inhibit protein translation of intracellular hepatitis C. Ultimately, the stabilization of G4 RNA in the genome of hepatitis C represents a promising new strategy for anti–hepatitis C drug development.
- Published
- 2016
47. Small-Molecule-Triggered and Light-Controlled Reversible Regulation of Enzymatic Activity
- Author
-
Jiaqi Wang, Anling Li, Zhiyong He, Yanyan Song, Shaoru Wang, Boshi Fu, Xiang Zhou, Tian Tian, Xin Zhou, and Xianqun Xu
- Subjects
Circular dichroism ,Light ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Small Molecule Libraries ,chemistry.chemical_compound ,Enzyme activator ,Structure-Activity Relationship ,Colloid and Surface Chemistry ,Molecular recognition ,Humans ,Blood Coagulation ,Molecular switch ,biology ,Dose-Response Relationship, Drug ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Circular Dichroism ,Thrombin ,General Chemistry ,DNA ,Telomere ,Small molecule ,Enzyme assay ,0104 chemical sciences ,Enzyme Activation ,Biophysics ,biology.protein ,Azo Compounds - Abstract
The fine control of enzyme activity is essential for the regulation of many important cellular and organismal functions. The light-regulation of proteins serves as an important method for the spatiotemporal control over the production and degradation of an enzyme product. This area is of intense interest for researchers. To the best of our knowledge, the use of small molecules as light-triggered molecular switches to reversibly control enzyme activity at the protein level has not yet been studied. In the present study, we demonstrate the light-controlled reversible regulation of the enzyme using a small-molecule-triggered switch, which is based on molecular recognition between an azobenzene derivative and telomere DNA. This molecule interconverts between the trans and cis states under alternate 365 nm UV and visible light irradiation, which consequently triggers the compaction and extension of telomere DNA. We further provide direct evidence for this structural switch using a circular dichroism study. Furthermore, our strategy has been successfully used to effectively control blood clotting in human plasma.
- Published
- 2016
48. Application of Micro Lesson in qBasics of Computer Applicationq Teaching
- Author
-
Shaoru Wang
- Subjects
Engineering ,Multimedia ,business.industry ,computer.software_genre ,business ,computer - Published
- 2016
49. Application of Project Teaching Method in qComputer Assembly and Maintenanceq Course Teaching
- Author
-
Shaoru Wang
- Subjects
Engineering ,business.industry ,Teaching method ,Mathematics education ,business ,Course (navigation) - Published
- 2016
50. Label-free detection of pH based on the i-motif using an aggregation-caused quenching strategy
- Author
-
Dongsheng Bai, Xiang Zhou, Yang Wang, Yalun Xie, Jinguo Huang, Boshi Fu, and Shaoru Wang
- Subjects
Sensor system ,Chemistry ,Allosteric regulation ,Metals and Alloys ,Nanotechnology ,General Chemistry ,Biocompatible material ,Ph changes ,Fluorescence ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Materials Chemistry ,Ceramics and Composites ,Biophysics ,DNA ,Label free - Abstract
A label-free and biocompatible pH sensor system based on the aggregation-caused quenching (ACQ) probe has been reported herein. The DNA i-motif, a kind of pH-triggered structure, affects the aggregation of PTCDI derivatives by structural switch that would provide significant fluorescence signals responding to the different pH values. Our method not only shows sensitive and reversible response to pH changes, but also could expand the detection range by allosteric control of the DNA i-motif.
- Published
- 2015
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