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Reversible manipulation of the G-quadruplex structures and enzymatic reactions through supramolecular host–guest interactions
- Source :
- Nucleic Acids Research
- Publication Year :
- 2017
- Publisher :
- Oxford University Press, 2017.
-
Abstract
- Supramolecular chemistry addresses intermolecular forces and consequently promises great flexibility and precision. Biological systems are often the inspirations for supramolecular research. The G-quadruplex (G4) belongs to one of the most important secondary structures in nucleic acids. Until recently, the supramolecular manipulation of the G4 has not been reported. The present study is the first to disclose a supramolecular switch for the reversible control of human telomere G4s. Moreover, this supramolecular switch has been successfully used to manipulate an enzymatic reaction. Using various methods, we show that cucurbit[7]uril preferably locks and encapsulates the positively charged piperidines of Razo through supramolecular interactions. They can switch the conformations of the DNA inhibitor between a flexible state and the rigid G4 and are therefore responsible for the reversible control of the thrombin activity. Thus, our findings open a promising route and exhibit potential applications in future studies of chemical biology.
- Subjects :
- Bridged-Ring Compounds
Future studies
Supramolecular chemistry
Chemical biology
macromolecular substances
Biology
010402 general chemistry
G-quadruplex
01 natural sciences
Molecular Docking Simulation
Enzyme catalysis
Chemical Biology and Nucleic Acid Chemistry
Piperidines
Genetics
Humans
Base Sequence
010405 organic chemistry
Intermolecular force
technology, industry, and agriculture
Imidazoles
Thrombin
DNA
Telomere
Combinatorial chemistry
0104 chemical sciences
G-Quadruplexes
Biochemistry
Nucleic acid
Azo Compounds
Subjects
Details
- Language :
- English
- ISSN :
- 13624962 and 03051048
- Volume :
- 45
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....ef8de5f6bf763f8b6b6a94cf7cb61145