Search

Your search keyword '"Shaohe Zhou"' showing total 5 results
Start Over Author "Shaohe Zhou"
5 results on '"Shaohe Zhou"'

1. Alantolactone induces apoptosis through ROS-mediated AKT pathway and inhibition of PINK1-mediated mitophagy in human HepG2 cells

Author

Xing Kang, Hijuan Wang, Yanwei Li, Ying Xiao, Lili Zhao, Tingting Zhang, Shaohe Zhou, Xiaolun Zhou, Yi Li, Zhexing Shou, Chao Chen, and Bin Li

Subjects
Alantolactone, apoptosis, ROS, mitophagy, AKT, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5
Abstract

Alantolactone (Ala), a major sesquiterpene lactone extracted from Inula helenium, exerts potent anti-tumour activities in various cancers. However, the underlying mechanism of such activities is still ambiguous. This study focused on evaluating the anti-tumour effects and molecular mechanisms of Ala on HepG2 cells. Our results demonstrated that Ala might inhibit cellular proliferation, induce G2/M phase arrest and apoptosis in HepG2 cells. Specifically, this study confirmed that Ala induced G2/M phase arrest by upregulating p21, downregulating cyclin A1 and cyclin B1, and promoting cellular apoptosis by increasing the expression of cleaved caspase-3 and PARP. Furthermore, Ala caused an increase in reactive oxygen species (ROS) level and inhibition of ROS production significantly prevented Ala-induced apoptosis. Interestingly, the accumulation of ROS, in turn, suppressed the downstream AKT signalling. Finally, mitophagy of Ala-treated HepG2 cells was observed by Mito/Lyso staining. Mitophagy was significantly inhibited by downregulation of the expression of PINK1 and Parkin proteins. The inhibition of mitophagy by a mitophagy inhibitor was found to markedly enhance Ala-mediated apoptosis and growth inhibition in HepG2 cells. Consequently, Ala induced cellular apoptosis via ROS-mediated suppression of AKT signalling and inhibition of PINK1-mediated mitophagy. Thus, Ala has potential to be used for the treatment of liver cancer.

Published
2019
Full Text
View/download PDF

2. A New Role for LOC101928437 in Non-Syndromic Intellectual Disability: Findings from a Family-Based Association Test.

Author

Shaohe Zhou, Zhangyan Shi, Meng Cui, Junlin Li, Zhe Ma, Yuanyu Shi, Zijian Zheng, Fuchang Zhang, Tianbo Jin, Tingting Geng, Chao Chen, Yale Guo, Jianping Zhou, Shaoping Huang, Xingli Guo, Lin Gao, Pingyuan Gong, Xiaocai Gao, and Kejin Zhang

Subjects
Medicine, Science
Abstract

Non-syndromic intellectual disability (NSID) is mental retardation in persons of normal physical appearance who have no recognisable features apart from obvious deficits in intellectual functioning and adaptive ability; however, its genetic etiology of most patients has remained unknown. The main purpose of this study was to fine map and identify specific causal gene(s) by genotyping a NSID family cohort using a panel of markers encompassing a target region reported in a previous work. A total of 139 families including probands, parents and relatives were included in the household survey, clinical examinations and intelligence tests, recruited from the Qinba mountain region of Shannxi province, western China. A collection of 34 tagged single nucleotide polymorphisms (tSNPs) spanning five microsatellite marker (STR) loci were genotyped using an iPLEX Gold assay. The association between tSNPs and patients was analyzed by family-based association testing (FBAT) and haplotype analysis (HBAT). Four markers (rs5974392, rs12164331, rs5929554 and rs3116911) in a block that showed strong linkage disequilibrium within the first three introns of the LOC101928437 locus were found to be significantly associated with NSID (all P

Published
2015
Full Text
View/download PDF

3. Alantolactone induces apoptosis through ROS-mediated AKT pathway and inhibition of PINK1-mediated mitophagy in human HepG2 cells

Author

Ying Xiao, Shaohe Zhou, Lili Zhao, Yi Li, Zhexing Shou, Yanwei Li, Bin Li, Tingting Zhang, Hijuan Wang, Xing Kang, Chao Chen, and Xiaolun Zhou

Subjects
Biomedical Engineering, Pharmaceutical Science, Medicine (miscellaneous), Apoptosis, 02 engineering and technology, Parkin, 03 medical and health sciences, chemistry.chemical_compound, Lactones, 0302 clinical medicine, Mitophagy, Humans, Sesquiterpenes, Eudesmane, Cyclin B1, Protein kinase B, PI3K/AKT/mTOR pathway, Cell Proliferation, chemistry.chemical_classification, Reactive oxygen species, General Medicine, Hep G2 Cells, 021001 nanoscience & nanotechnology, Phosphoproteins, Cell biology, G2 Phase Cell Cycle Checkpoints, chemistry, 030220 oncology & carcinogenesis, M Phase Cell Cycle Checkpoints, Growth inhibition, 0210 nano-technology, Reactive Oxygen Species, Protein Kinases, Proto-Oncogene Proteins c-akt, Biotechnology
Abstract

Alantolactone (Ala), a major sesquiterpene lactone extracted from Inula helenium, exerts potent anti-tumour activities in various cancers. However, the underlying mechanism of such activities is still ambiguous. This study focused on evaluating the anti-tumour effects and molecular mechanisms of Ala on HepG2 cells. Our results demonstrated that Ala might inhibit cellular proliferation, induce G2/M phase arrest and apoptosis in HepG2 cells. Specifically, this study confirmed that Ala induced G2/M phase arrest by upregulating p21, downregulating cyclin A1 and cyclin B1, and promoting cellular apoptosis by increasing the expression of cleaved caspase-3 and PARP. Furthermore, Ala caused an increase in reactive oxygen species (ROS) level and inhibition of ROS production significantly prevented Ala-induced apoptosis. Interestingly, the accumulation of ROS, in turn, suppressed the downstream AKT signalling. Finally, mitophagy of Ala-treated HepG2 cells was observed by Mito/Lyso staining. Mitophagy was significantly inhibited by downregulation of the expression of PINK1 and Parkin proteins. The inhibition of mitophagy by a mitophagy inhibitor was found to markedly enhance Ala-mediated apoptosis and growth inhibition in HepG2 cells. Consequently, Ala induced cellular apoptosis via ROS-mediated suppression of AKT signalling and inhibition of PINK1-mediated mitophagy. Thus, Ala has potential to be used for the treatment of liver cancer.

Published
2019

5. A New Role for LOC101928437 in Non-Syndromic Intellectual Disability: Findings from a Family-Based Association Test

Author

Tianbo Jin, Zhangyan Shi, Yale Guo, Shaoping Huang, Shaohe Zhou, Tingting Geng, Meng Cui, Junlin Li, Zhe Ma, Fuchang Zhang, Kejin Zhang, Zijian Zheng, Lin Gao, Chao Chen, Yuanyu Shi, Xingli Guo, Xiaocai Gao, Jianping Zhou, and Pingyuan Gong

Subjects
Adult, Genetic Markers, Male, Linkage disequilibrium, Candidate gene, China, RNA, Untranslated, Adolescent, lcsh:Medicine, Single-nucleotide polymorphism, Locus (genetics), Biology, Polymorphism, Single Nucleotide, Young Adult, Borderline intellectual functioning, Asian People, Gene Frequency, Intellectual Disability, Intellectual disability, medicine, Humans, Family, Genetic Predisposition to Disease, lcsh:Science, Child, Allele frequency, Genetic Association Studies, Aged, Genetics, Multidisciplinary, lcsh:R, Haplotype, Middle Aged, medicine.disease, Haplotypes, Child, Preschool, lcsh:Q, Female, Microsatellite Repeats, Research Article
Abstract

Non-syndromic intellectual disability (NSID) is mental retardation in persons of normal physical appearance who have no recognisable features apart from obvious deficits in intellectual functioning and adaptive ability; however, its genetic etiology of most patients has remained unknown. The main purpose of this study was to fine map and identify specific causal gene(s) by genotyping a NSID family cohort using a panel of markers encompassing a target region reported in a previous work. A total of 139 families including probands, parents and relatives were included in the household survey, clinical examinations and intelligence tests, recruited from the Qinba mountain region of Shannxi province, western China. A collection of 34 tagged single nucleotide polymorphisms (tSNPs) spanning five microsatellite marker (STR) loci were genotyped using an iPLEX Gold assay. The association between tSNPs and patients was analyzed by family-based association testing (FBAT) and haplotype analysis (HBAT). Four markers (rs5974392, rs12164331, rs5929554 and rs3116911) in a block that showed strong linkage disequilibrium within the first three introns of the LOC 101928437 locus were found to be significantly associated with NSID (all P

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results