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7 results on '"Shang-Qing Liu"'

1. Seasonal response of nitrogen exchange fluxes to crab disturbance at sediment–water interface in coastal tidal wetlands

Author

Guo-fen Hua, Shang-qing Liu, Xiang-dong Liu, Jin-li Li, Yue Fang, Wen-ting Xie, and Xiang Xu

Subjects
Coastal tidal flat wetland, Crab disturbance, Nitrogen flux, Nitrification, Denitrification, Sediment–water interface, River, lake, and water-supply engineering (General), TC401-506
Abstract

Coastal wetlands are hotspots for nitrogen (N) cycling, and crab burrowing is known to transform N in intertidal marsh soils. However, the underlying mechanisms remain unclear. This study conducted field experiments and used indoor control test devices to investigate the seasonal response of nitrogen to crab disturbance at the sediment–water interface in coastal tidal flat wetlands. The results showed that crab disturbance exhibited significant seasonality with large seasonal differences in cave density and depth. Due to crab disturbance, nitrogen fluxes at the sediment–water interface were much greater in the box with crabs than in the box without crabs. In summer, NH4+-N showed a positive flux from the sediment to the overlying water, but NO2−-N and NO3−-N showed positive fluxes from the sediment to the overlying water only in early stages. In winter, NH4+-N showed a positive flux from the sediment to the overlying water, but NO2−-N and NO3−-N both exhibited positive and negative fluxes. These results indicated that the presence of crab burrows can cause the aerobic layer to move downward by approximately 8–15 cm in summer and directly promote nitrification at the sediment surface.

Published
2024
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2. Corticotropin‐releasing factor is involved in acute stress‐induced analgesia and antipruritus

Author

Xiao‐Dong Wang, Hao‐Miao Bai, Xiao‐Lan Li, Lin‐Fang Zhang, Fei Li, Yang Bai, Zhen‐Yu Wu, Shang‐Qing Liu, and Hui Li

Subjects
analgesia, antipruritus, CRF, itch, pain, stress, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Abstract Background Under the condition of stress, the hypothalamic–pituitary–adrenal axis (HPA axis) is activated and causes the secretion of corticotropin‐releasing factor (CRF). Previous studies have demonstrated that CRF is involved in the regulation of pain and itch. Thus, it remains worthy to explore whether the desensitization of pain and itch under high‐intensity acute stress (such as high fear and tension) is related to the sharp increase of CRF. Methods Forced swimming was used to simulate acute stress. ELISA and pharmacological methods were conducted to observe the effects of forced swimming on acute pain or itch and the relationship between blood CRF content and itch or pain behavior. Intracerebroventricular (ICV) administration of CRF was conducted to examine the effects of CRF on acute pain or itch. Intrathecal administration of CRF receptor agonist or antagonist was conducted to examine the receptor mechanisms of the regulatory role of CRF in pain and itch. Results ELISA experiment showed that the serum CRF in mice reached its peak within 5–10 min after acute stress (forced swimming). Behavioral data showed that the scratching behavior induced by itch agents decreased after acute swimming, while the mechanical pain threshold increased significantly. The inhibitory effect of acute stress on pain and itch is mediated by CRF receptor2 (CRFR2). Then, ICV injection of CRF was used to simulate the massive release of CRF under acute stress, and we observed that the scratching behavior induced by histamine or chloroquine was significantly inhibited after ICV injection of CRF. The above effects of CRF are mainly mediated by CRFR2. These results suggest that 5–10 min after acute stress, a large amount of CRF is released into the blood from the hypothalamus, which significantly inhibits acute pain and itch by acting on CRFR2. ICV injection of CRF can replicate the antipruritus effects of acute stress. Conclusions The present study investigated the mechanism of acute stress‐induced analgesia and antipruritus and provided theoretical support for the treatment of pain and itch.

Published
2022
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5. Comparative Proteome Analysis of Breast Cancer and Adjacent Normal Breast Tissues in Human

Author

Hui-Jun Yang, Tian Yong Xing, Shi Shan Deng, Ruo Hong Xiong, You Guang Lu, Shang Qing Liu, Hong Ying Zhou, and Bin Wen

Subjects
Adult, Models, Molecular, Proteomics, Serum albumin, Cathepsin D, Breast Neoplasms, Biochemistry, Article, Mass Spectrometry, breast cancer, Breast cancer, Biomarkers, Tumor, Genetics, medicine, Humans, Electrophoresis, Gel, Two-Dimensional, Breast, skin and connective tissue diseases, Molecular Biology, Polyacrylamide gel electrophoresis, biology, business.industry, Tumor Protein, Translationally-Controlled 1, Middle Aged, Prognosis, medicine.disease, Gene Expression Regulation, Neoplastic, Computational Mathematics, Matrix-assisted laser desorption/ionization, 2D-PAGE, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Immunology, Proteome, biology.protein, Cancer research, biomarker, Biomarker (medicine), Female, molecular mechanism, business
Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS), incorporated with online database searching, were performed to investigate differential proteins of breast cancer and adjacent normal breast tissues. Considering that serum albumin is abundantly presented in normal control samples, 15 differential spots detected in 11 out of 12 (91.7%) breast cancer samples were identified by online SIENA-2DPAGE database searching and MALDI-TOF/TOF-MS analysis. The results indicate that pathological changes of breast cancer are concerned with augmentation of substance metabolism, promotion of proteolytic activity, decline of activity of some inhibitors of enzymes, and so on. Some important proteins involved in the pathological process of breast cancer with changed expression may be useful biomarkers, such as alpha-1-antitrypsin, EF-1-beta, cathepsin D, TCTP, SMT3A, RPS12, and PSMA1, among which SMT3A, RPS12, and PSMA1 were first reported for breast cancer in this study.

Published
2006

6. Down-regulation of alpha-2u globulin in renal mitochondria of STZ-induced diabetic rats observed by a proteomic method

Author

Li Chen, Chang-Ping Cai, Rong Cai, Quan-Bo Zhang, Shi-He Sun, and Shang-Qing Liu

Subjects
Male, Proteomics, medicine.medical_specialty, Globulin, Proteome, Endocrinology, Diabetes and Metabolism, Down-Regulation, Mitochondrion, Validation Studies as Topic, Kidney, Streptozocin, Diabetes Mellitus, Experimental, Diabetic nephropathy, Mitochondrial Proteins, Rats, Sprague-Dawley, Endocrinology, Downregulation and upregulation, Diabetes mellitus, Internal medicine, Alpha-Globulins, medicine, Animals, Diabetic Nephropathies, Electrophoresis, Gel, Two-Dimensional, Polyacrylamide gel electrophoresis, chemistry.chemical_classification, biology, Fatty acid, General Medicine, medicine.disease, Mitochondria, Rats, medicine.anatomical_structure, chemistry, biology.protein
Abstract

Aim To identify the changes of mitochondrial protein expression in diabetic renal parenchyma and to characterize their molecular functions and biological processes in diabetes. Methods Mitochondrial proteins extracted from renal parenchyma mitochondria of streptozotocin-induced diabetic rats and normal rats were separated by two-dimensional polyacrylamide gel electrophoresis and identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. Results Eleven proteins from 533 visualized protein spots displayed significant different expressions in mitochondria of diabetic kidneys compared with those in normal ones. Among these altered proteins, two proteins with the most obvious changes in protein expression were identified as alpha-2u globulin (mature protein, named A2) and its proteolytically modified form (named A2-fragment) respectively. These proteins were found in mitochondria of male rat renal parenchyma and were proved to be down-regulated in diabetic rats simultaneously. Conclusion Our results suggest that down-regulation of alpha-2u globulin may be associated with an abnormal β-oxidation of long-chain fatty acids during diabetes. The decreased expression of A2-fragment in renal mitochondria of diabetic nephropathy may reduce fatty acid β-oxidation, which leads to a diminished energy supply from mitochondria to kidney tissue and the deposition of a large number of fatty acids in the kidney, ultimately causing and aggravating kidney damage. In conclusion, these findings may be helpful for understanding the molecular mechanism of diabetic nephropathy.

Published
2012

7. Differences in expression of retinal proteins between diabetic and normal rats

Author

Cheng-Jun Li, Jian Kang, Rong Cai, En-Jie Tang, Hui-Jun Yang, Shang-Qing Liu, and Bin Wen

Subjects
Guanylate kinase, Protein subunit, Diabetes Mellitus, Experimental, Rats, Sprague-Dawley, Random Allocation, chemistry.chemical_compound, Tandem Mass Spectrometry, Crystallin, Alloxan, Animals, Eye Proteins, Diabetic Retinopathy, ATP synthase, biology, Gene Expression Profiling, Gastroenterology, Retinal, General Medicine, Rats, Disease Models, Animal, Biochemistry, chemistry, biology.protein, Electrophoresis, Polyacrylamide Gel, Female, Isoelectric Focusing, Retinal Rod Photoreceptor Cells, Signal transduction, Rapid Communication
Abstract

AIM: To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes. METHODS: Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (P < 0.05) were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics. RESULTS: 2-DE showed that the expression was up-regulated in 5 retinal proteins, down-regulated in 23 retinal proteins, and disappeared in 8 retinal proteins. Eight spots were identified from the 36 spots by tandem mass spectrometry (MS/MS) and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells. Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far. CONCLUSION: There are differences in expression of retinal proteins between diabetic and normal rats. These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.

Published
2007

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