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Down-regulation of alpha-2u globulin in renal mitochondria of STZ-induced diabetic rats observed by a proteomic method
- Source :
- Annales d'endocrinologie. 73(6)
- Publication Year :
- 2012
-
Abstract
- Aim To identify the changes of mitochondrial protein expression in diabetic renal parenchyma and to characterize their molecular functions and biological processes in diabetes. Methods Mitochondrial proteins extracted from renal parenchyma mitochondria of streptozotocin-induced diabetic rats and normal rats were separated by two-dimensional polyacrylamide gel electrophoresis and identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. Results Eleven proteins from 533 visualized protein spots displayed significant different expressions in mitochondria of diabetic kidneys compared with those in normal ones. Among these altered proteins, two proteins with the most obvious changes in protein expression were identified as alpha-2u globulin (mature protein, named A2) and its proteolytically modified form (named A2-fragment) respectively. These proteins were found in mitochondria of male rat renal parenchyma and were proved to be down-regulated in diabetic rats simultaneously. Conclusion Our results suggest that down-regulation of alpha-2u globulin may be associated with an abnormal β-oxidation of long-chain fatty acids during diabetes. The decreased expression of A2-fragment in renal mitochondria of diabetic nephropathy may reduce fatty acid β-oxidation, which leads to a diminished energy supply from mitochondria to kidney tissue and the deposition of a large number of fatty acids in the kidney, ultimately causing and aggravating kidney damage. In conclusion, these findings may be helpful for understanding the molecular mechanism of diabetic nephropathy.
- Subjects :
- Male
Proteomics
medicine.medical_specialty
Globulin
Proteome
Endocrinology, Diabetes and Metabolism
Down-Regulation
Mitochondrion
Validation Studies as Topic
Kidney
Streptozocin
Diabetes Mellitus, Experimental
Diabetic nephropathy
Mitochondrial Proteins
Rats, Sprague-Dawley
Endocrinology
Downregulation and upregulation
Diabetes mellitus
Internal medicine
Alpha-Globulins
medicine
Animals
Diabetic Nephropathies
Electrophoresis, Gel, Two-Dimensional
Polyacrylamide gel electrophoresis
chemistry.chemical_classification
biology
Fatty acid
General Medicine
medicine.disease
Mitochondria
Rats
medicine.anatomical_structure
chemistry
biology.protein
Subjects
Details
- ISSN :
- 22133941
- Volume :
- 73
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Annales d'endocrinologie
- Accession number :
- edsair.doi.dedup.....e7fbf3ce589f98e50e3ca30290d1fd0f