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543 results on '"Shafferman, Avigdor"'

13. Evaluation of Protective Immunity Induced by Yersinia enterocolitica Type-III Secretion System Mutants

Author

Mamroud, Emanuelle, Flashner, Yehuda, Tidhar, Avital, Ber, Raphael, Gur, David, Aftalion, Moshe, Lazar, Shirley, Velan, Baruch, Shafferman, Avigdor, Cohen, Sara, Back, Nathan, editor, Cohen, Irun R., editor, Kritchevsky, David, editor, Lajtha, Abel, editor, Paoletti, Rodolfo, editor, Skurnik, Mikael, editor, Bengoechea, José Antonio, editor, and Granfors, Kaisa, editor

Published
2003
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15. Recommendations for Nomenclature in Cholinesterases

Author

Massoulié, Jean, Sussman, Joel L., Doctor, Bhupendra P., Soreq, Hermona, Velan, Baruch, Cygler, Miroslaw, Rotundo, Richard, Shafferman, Avigdor, Silman, Israel, Taylor, Palmer, Shafferman, Avigdor, editor, and Velan, Baruch, editor

Published
1992
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27. Identification of Genes Involved in Yersinia pestis Virulence by Signature-tagged Mutagenesis

Author

Flashner, Yehuda, Mamroud, Emanuelle, Tidhar, Avital, Ber, Raphael, Aftalion, Moshe, Gur, David, Zvi, Anat, Ariel, Naomi, Velan, Baruch, Shafferman, Avigdor, Cohen, Sara, Back, Nathan, editor, Cohen, Irun R., editor, Kritchevsky, David, editor, Lajtha, Abel, editor, Paoletti, Rodolfo, editor, Skurnik, Mikael, editor, Bengoechea, José Antonio, editor, and Granfors, Kaisa, editor

Published
2003
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35. Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome C[O.sub.2]-dependent cross talk mechanisms modulate extracellular proteolytic activities

Author

Chitlaru, Theodor, Gat, Orit, Gozlan, Yael, Ariel, Naomi, and Shafferman, Avigdor

Subjects
Bacillus anthracis -- Genetic aspects, Proteolysis -- Research, Genetic research, Biological sciences
Abstract

The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique pattern of protein relative abundance with respect to the presence of the virulence plasmids was revealed. In minimal medium under high C[O.sub.2] tension, conditions considered to simulate those encountered in the host, the presence of the plasmids leads to enhanced expression of 12 chromosome-carried genes (10 of which could not be detected in the absence of the plasmids) in addition to expression of 5 pXO1-encoded proteins. Furthermore, under these conditions, the presence of the pXO1 and pXO2 plasmids leads to the repression of 14 chromosomal genes. On the other hand, in minimal aerobic medium not supplemented with C[O.sub.2], the virulent and avirulent B. anthracis strains manifest very similar protein signatures, and most strikingly, two proteins (the metalloproteases InhA1 and NprB, orthologs of gene products attributed to the Bacillus cereus group PlcR regulon) represent over 90% of the total secretome. Interestingly, of the 64 identified gene products, at least 31 harbor features characteristic of virulence determinants (such as toxins, proteases, nucleotidases, sulfatases, transporters, and detoxification factors), 22 of which are differentially regulated in a plasmid-dependent manner. The nature and the expression patterns of proteins in the various secretomes suggest that distinct C[O.sub.2]-responsive chromosome- and plasmid-encoded regulatory factors modulate the secretion of potential novel virulence factors, most of which are associated with extracellular proteolytic activities.

Published
2006

36. Vaccination with Plasmid DNA Expressing the Yersinia pestis Capsular Protein F1 Protects Mice Against Plague

Author

Grosfeld, Haim, Bino, Tamar, Flashner, Yehuda, Ber, Raphael, Mamroud, Emanuelle, Lustig, Shlomo, Velan, Baruch, Shafferman, Avigdor, Cohen, Sara, Back, Nathan, editor, Cohen, Irun R., editor, Kritchevsky, David, editor, Lajtha, Abel, editor, Paoletti, Rodolfo, editor, Skurnik, Mikael, editor, Bengoechea, José Antonio, editor, and Granfors, Kaisa, editor

Published
2003
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38. The aromatic 'trapping' of the catalytic histidine is essential for efficient catalysis in acetylcholinesterase

Author

Barak, Dov, Kaplan, Dana, Ordentlich, Arie, Ariel, Naomi, Velan, Baruch, and Shafferman, Avigdor

Subjects
Biochemistry -- Research, Aromatic compounds -- Physiological aspects, Histidine -- Physiological aspects, Catalysts -- Physiological aspects, Acetylcholinesterase -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the catalytic histidine 447. The nature of the putitive array of interactions optimizing histidine 447 orientation has been explored and the details are reported.

Published
2002

39. Does 'butyrylization' of acetylcholinesterase through substitution of the six divergent aromatic amino acids in the active center gorge generate an enzyme mimic of butyrylcholinesterase?

Author

Kaplan, Dana, Ordentlich, Arie, Barak, Dov, Ariel, Naomi, Kronman, Chanoch, Velan, Baruch, and Shafferman, Avigdor

Subjects
Biochemistry -- Research, Aromatic compounds -- Research, Amino acids -- Research, Enzymes -- Research, Biological sciences, Chemistry
Abstract

Research has been conducted on the active center gorge of the human acetylcholinesterase and human butyrylcholinesterase. The reactivity difference between these enzymes has been investigated.

Published
2001

42. Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains

Author

Zaide, Galia, primary, Elia, Uri, additional, Cohen-Gihon, Inbar, additional, Israeli, Ma’ayan, additional, Rotem, Shahar, additional, Israeli, Ofir, additional, Ehrlich, Sharon, additional, Cohen, Hila, additional, Lazar, Shirley, additional, Beth-Din, Adi, additional, Shafferman, Avigdor, additional, Zvi, Anat, additional, Cohen, Ofer, additional, and Chitlaru, Theodor, additional

Published
2020
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44. Transition state structure and rate of determination for the acylation stage of acetylcholinesterase catalyzed hydrolysis of (acetylthio)choline

Author

Malany, Siobhan, Sawai, Monali, Sikorski, R. Steven, Seravalli, Javier, Quinn, Daniel M., Radic, Zoran, Taylor, Palmer, Kronman, Chanoch, Velan, Baruch, and Shafferman, Avigdor

Subjects
Acetylcholinesterase -- Research, Acylation -- Research, Biochemistry -- Research, Chemistry
Abstract

Research into the transition state structure and rate-limiting steps for the acylation stage of acetylcholinesterase-catalyzed hydrolysis of (acetylthio)choline is presented.

Published
2000

45. Evidence for P-N bond scission in phosphoroamidate nerve agent adducts of human acetylcholinesterase

Author

Barak, Dov, Ordentlich, Arie, Kaplan, Dana, Barak, Ruth, Mizrahi, Dana, Kronman, Chanoch, Segall, Yoffi, Velan, Baruch, and Shafferman, Avigdor

Subjects
Acetylcholinesterase -- Research, Toxins -- Physiological aspects, Nerve gas -- Physiological aspects, Mass spectrometry -- Usage, Biological sciences, Chemistry
Abstract

Results show that human acetylcholinesterase adducts containing organophosphorous toxic agents with P-N derivation and those with phosphonates age by different mechanisms. Present study is the first report to show that acetylcholinesterase adducts containing toxic P-N agents age through P-N bond scission.

Published
2000

46. Crystal structures of aged phosphonylated acetylcholinesterase: nerve agent reaction products at the atomic level

Author

Millard, Charles B., Kryger, Gitay, Ordentlich, Arie, Greenblatt, Harry M., Harel, Michal, Raves, Mia L., Segall, Yoffi, Barak, Dov, Shafferman, Avigdor, Silman, Israel, and Sussman, Joel L.

Subjects
Acetylcholinesterase -- Research, Anhydrides -- Research, Esters -- Research, Irreversible processes -- Research, Biological sciences, Chemistry
Abstract

A study on acetylcholinesterase was conducted to solve the three-dimensional structures of aged phosphylated Torpedo californica acetylcholinesterase (TcAChE) to understand the forces that oppose reactivation or dephosphylation of the organophosphorus acid anhydride. Further, the experiment also provides structural models for the deacylation tetrahedral intermediate of carboxyl ester hydrolysis. No major structural rearrangement in TcAChE were detected as a result of the aging reaction itself.

Published
1999

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