Search

Your search keyword '"Sawaid Kaiyal, Raneen"' showing total 5 results
Start Over Author "Sawaid Kaiyal, Raneen"
5 results on '"Sawaid Kaiyal, Raneen"'

1. Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach.

Author

Sawaid Kaiyal, Raneen, Mukherjee, Sromona D., Panner Selvam, Manesh Kumar, Miller, Aaron W., Vij, Sarah C., and Lundy, Scott D.

Subjects
SPERMATOZOA analysis, PROTEIN analysis, SEMEN analysis, RISK assessment, SUPEROXIDE dismutase, GLUTATHIONE, MITOCHONDRIA, LIQUID chromatography-mass spectrometry, RESEARCH funding, INFERTILITY, PILOT projects, OXIDATIVE stress, BIOINFORMATICS, PROTEOMICS, WESTERN immunoblotting, SPERM donation, OXIDOREDUCTASES, MITOCHONDRIAL pathology, ELECTROPHORESIS, COMPARATIVE studies, DISEASE risk factors, DISEASE complications
Abstract

Research question: Male infertility accounts for almost half of all infertility cases worldwide, with idiopathic male infertility accounting for up to 30% of the cases. Sperm proteomics has revealed critical molecular pathway changes in men with infertility. However, the sperm mitochondrial proteome remains poorly understood. We attempted to answer the following question: Do patients with idiopathic primary male infertility exhibit a proteomic signature associated with mitochondrial dysfunction that could be used as a target for future mechanistic investigations? Design: Patients with idiopathic primary infertility (20–40 years old) referred to the Cleveland Clinic between March 2012 and April 2014 were compared with fertile donor controls. Sperm proteins were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis page (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS), and differentially expressed proteins (DEPs) were identified based on significance test results and fold change thresholds. Protein expression was validated using western blotting. Results: Proteomic analysis of pooled samples from fertile donors (n = 5) and patients with idiopathic primary infertility (n = 5) identified 1,134 proteins, including 344 DEPs. Mitochondrial dysfunction topped the ingenuity toxicity list. Analysis of expression levels of three mitochondrial proteins known to combat oxidative stress revealed that peroxiredoxin-5 (PRDX5) and superoxide dismutase 2 (SOD2), but not glutathione disulphide reductase, were significantly decreased in patient samples compared with those in fertile-donor samples. Conclusions: This study revealed an association of downregulated expression of PRDX5 and SOD2 in sperm samples of patients with idiopathic primary male infertility. Our results support future mechanistic studies and development of advanced diagnostic methods to better identify men with mitochondria-related male infertility. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

2. Antioxidants for male infertility: therapeutic scheme and indications. A retrospective single-center real-life study

Author

CANNARELLA, Rossella, primary, CRAFA, Andrea, additional, SAWAID KAIYAL, Raneen, additional, KURODA, Shinnosuke, additional, BARBAGALLO, Federica, additional, ALAMO, Angela, additional, MONGIOÌ, Laura M., additional, SAPIENZA, Sabrina, additional, CONDORELLI, Rosita A., additional, LA VIGNERA, Sandro, additional, and CALOGERO, Aldo E., additional

Published
2024
Full Text
View/download PDF

3. Comparative motility assessment of sperm retrieved from micro‐testicular sperm extraction: A single‐centre study comparing fresh and frozen–thawed sperm.

Author

Sawaid Kaiyal, Raneen, Bryk, Darren J., Kuroda, Shinnosuke, Doolittle, Johnathan, Parekh, Neel V., Sabanegh, Edmund, Desai, Nina, Lundy, Scott D., and Vij, Sarah C.

Subjects
*MALE infertility, *SPERM motility, *AZOOSPERMIA, *PATIENT selection, *INTRACYTOPLASMIC sperm injection
Abstract

Introduction Materials and Methods Results Conclusions Microsurgical testicular sperm extraction (microTESE) is crucial for treating non‐obstructive azoospermia (NOA), offering both ‘fresh’ and ‘frozen’ options. This study evaluates the impact of fresh versus frozen microTESE on the progression to intra‐cytoplasmic sperm injection (ICSI) cycles, focusing on sperm motility.We conducted a retrospective analysis of microTESE procedures at a major medical centre from 2007 to 2021, excluding cases of obstructive azoospermia and cryptozoospermia. Patients were divided into two groups: fresh microTESE (Group FR) and frozen microTESE (Group FZ). Sperm motility was assessed, and ICSI outcomes were compared between groups.Out of 128 microTESE procedures on 113 NOA patients, 31 were fresh and 97 were frozen. Sperm was found in 67.7% of fresh cases and 45.3% of frozen cases. In fresh cases, 85.7% had motile sperm for ICSI, whereas in frozen cases, 81.8% had motile sperm initially, but only 52.7% retained motility post‐thaw.Our findings indicate a significant drop in motile sperm availability for ICSI in frozen microTESE cases compared to fresh ones. This suggests a potential advantage of fresh microTESE for certain couples, despite the logistical challenges, highlighting the need for careful patient selection and counselling. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

4. Controversy and consensus on the management of elevated sperm DNA fragmentation in male infertility: a global survey, current guidelines, and expert recommendations

Author

Ala'a Farkouh, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Parviz Kavoussi, Ramadan Saleh, Armand Zini, Mohamed Arafa, Ahmed M Harraz, Murat Gul, Vilvapathy Senguttuvan Karthikeyan, Damayanthi Durairajanayagam, Amarnath Rambhatla, Florence Boitrelle, Eric Chung, Ponco Birowo, Tuncay Toprak, Ramy Abou Ghayda, Rossella Cannarella, Nguyen Ho Vinh Phuoc, Fotios Dimitriadis, Giorgio I Russo, Ioannis Sokolakis, Taymour Mostafa, Konstantinos Makarounis, Imad Ziouziou, Shinnosuke Kuroda, Marion Bendayan, Raneen Sawaid Kaiyal, Andrian Japari 33, Mara Simopoulou 34, Lucia Rocco, Nicolas Garrido 36, Nazim Gherabi 37, Kadir Bocu 38, Oguzhan Kahraman 39, Tan V Le 27 28, Christine Wyns 40, Kelton Tremellen 41, Selcuk Sarikaya 42, Sheena Lewis 43, Donald P Evenson 44, Edmund Ko 45, Aldo E Calogero 25, Fahmi Bahar 46, Marlon Martinez 47, Andrea Crafa 25, Quang Nguyen 48 49, Rafael F Ambar 50 51, Giovanni Colpi 52, Mustafa Emre Bakircioglu 53, Ralf Henkel 54 55, Hussein Kandil 56, Ege Can Serefoglu 57, Abdullah Alarbid 13, Akira Tsujimura 58, Alireza Kheradmand 59, Christina Anagnostopoulou 60, Angelo Marino 61, Aram Adamyan 62, Birute Zilaitiene 63, Cevahir Ozer 39, Edoardo Pescatori 64, Paraskevi Vogiatzi 65, Gian Maria Busetto 66, Giancarlo Balercia 67, Haitham Elbardisi 9 10, Hamed Akhavizadegan 68, Hesamoddin Sajadi 69, Hisanori Taniguchi 70, Hyun Jun Park 71 72, Israel Maldonado Rosas 73, Mohamed Al-Marhoon 74, Mohammad Ali Sadighi Gilani 69, Naif Alhathal 75, Germar-Michael Pinggera 76, Priyank Kothari 77, Nasser Mogharabian 78, Sava Micic 79, Sheryl Homa 80, Sara Darbandi 81 82, Tran Quang Tien Long 83, Wael Zohdy 10, Widi Atmoko 22, Marjan Sabbaghian 69, Wael Ibrahim 84, Ryan P Smith 85, Christopher Chee Kong Ho 86, Jean de la Rosette 87, Ahmed I El-Sakka 88, Mirko Preto 89, Cătălina Zenoaga-Barbăroșie 90, Saad Mohammed Abumelha 91, Aykut Baser 92, Kaan Aydos 93, Liliana Ramirez-Dominguez 73, Vijay Kumar 94, Teng Aik Ong 95, Tiago Cesar Mierzwa 96, Ricky Adriansjah 97, Saleem A Banihani 98, Kasonde Bowa 99, Shinichiro Fukuhara 100, Marcelo Rodriguez Peña 101, Mohamad Moussa 102 103, Umut Çağın Ari 104, Chak-Lam Cho 105, Nicholas N Tadros 106, Muhammet Rasit Ugur 107, Edouard Amar 108, Marco Falcone 109, Frederic Romain Santer 76, Arif Kalkanli 110, Keshab Kumar Karna 111, Kareim Khalafalla 9 112 113, Ranjit B Vishwakarma 114, Federica Finocchi 67, Carlo Giulioni 115, Erman Ceyhan 39, Gökhan Çeker 116, Chadi Yazbeck 117, Osvaldo Rajmil 118, Mehmet Yilmaz 119, Baris Altay 120, Trenton L Barrett 121, Kay Seong Ngoo 122, Shubhadeep Roychoudhury 123, Gianmaria Salvio 67, Haocheng Lin 124, Ates Kadioglu 125, Massimiliano Timpano 109, Tomer Avidor-Reiss 126 127, Lukman Hakim 128, Puneet Sindhwani 128, Giorgio Franco 129, Rajender Singh 130, Filippo Giacone 131, Mikhail Ruzaev 132, Raghavender Kosgi 133, Nikolaos Sofikitis 134, Ayad Palani 135, Gokhan Calik 87, Deniz Kulaksız 136, Davor Jezek 137, Manaf Al Hashmi 138 139, Panagiotis Drakopoulos 140 141, Huda Omran 142, Sofia Leonardi 143, Ciler Celik-Ozenci 144, Nur Dokuzeylül Güngör 145, Jonathan Ramsay 146, Toshiyasu Amano 147, Emrullah Sogutdelen 148, Gede Wirya Kusuma Duarsa 149, Koji Chiba 150, Sunil Jindal 151, Missy Savira 22, Luca Boeri 152, Edson Borges 153, Deepak Gupte 154, Fatih Gokalp 155, Guadalupe Hernández Hebrard 73, Suks Minhas 156, Rupin Shah 114, Global Andrology Forum, Farkouh, Ala'A, Agarwal, Ashok, Abo-Almagd Abdel-Meguid Hamoda, Taha, Kavoussi, Parviz, Saleh, Ramadan, Zini, Armand, Arafa, Mohamed, M Harraz, Ahmed, Gul, Murat, Senguttuvan Karthikeyan, Vilvapathy, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Boitrelle, Florence, Chung, Eric, Birowo, Ponco, Toprak, Tuncay, Abou Ghayda, Ramy, Cannarella, Rossella, Ho Vinh Phuoc, Nguyen, Dimitriadis, Fotio, I Russo, Giorgio, Sokolakis, Ioanni, Mostafa, Taymour, Makarounis, Konstantino, Ziouziou, Imad, Kuroda, Shinnosuke, Bendayan, Marion, Sawaid Kaiyal, Raneen, Japari 33, Andrian, Simopoulou 34, Mara, Rocco, Lucia, Garrido 36, Nicola, Gherabi 37, Nazim, Bocu 38, Kadir, Kahraman 39, Oguzhan, V Le 27 28, Tan, Wyns 40, Christine, Tremellen 41, Kelton, Sarikaya 42, Selcuk, Lewis 43, Sheena, P Evenson 44, Donald, Ko 45, Edmund, E Calogero 25, Aldo, Bahar 46, Fahmi, Martinez 47, Marlon, Crafa 25, Andrea, Nguyen 48 49, Quang, F Ambar 50 51, Rafael, Colpi 52, Giovanni, Emre Bakircioglu 53, Mustafa, Henkel 54 55, Ralf, Kandil 56, Hussein, Can Serefoglu 57, Ege, Alarbid 13, Abdullah, Tsujimura 58, Akira, Kheradmand 59, Alireza, Anagnostopoulou 60, Christina, Marino 61, Angelo, Adamyan 62, Aram, Zilaitiene 63, Birute, Ozer 39, Cevahir, Pescatori 64, Edoardo, Vogiatzi 65, Paraskevi, Maria Busetto 66, Gian, Balercia 67, Giancarlo, 9 10, Haitham Elbardisi, Akhavizadegan 68, Hamed, Sajadi 69, Hesamoddin, Taniguchi 70, Hisanori, Jun Park 71 72, Hyun, Maldonado Rosas 73, Israel, Al-Marhoon 74, Mohamed, Ali Sadighi Gilani 69, Mohammad, Alhathal 75, Naif, Pinggera 76, Germar-Michael, Kothari 77, Priyank, Mogharabian 78, Nasser, Micic 79, Sava, Homa 80, Sheryl, Darbandi 81 82, Sara, Quang Tien Long 83, Tran, Zohdy 10, Wael, Atmoko 22, Widi, Sabbaghian 69, Marjan, Ibrahim 84, Wael, P Smith 85, Ryan, Chee Kong Ho 86, Christopher, de la Rosette 87, Jean, I El-Sakka 88, Ahmed, Preto 89, Mirko, Zenoaga-Barbăroșie 90, Cătălina, Mohammed Abumelha 91, Saad, Baser 92, Aykut, Aydos 93, Kaan, Ramirez-Dominguez 73, Liliana, Kumar 94, Vijay, Aik Ong 95, Teng, Cesar Mierzwa 96, Tiago, Adriansjah 97, Ricky, A Banihani 98, Saleem, Bowa 99, Kasonde, Fukuhara 100, Shinichiro, Rodriguez Peña 101, Marcelo, Moussa 102 103, Mohamad, Çağın Ari 104, Umut, Cho 105, Chak-Lam, N Tadros 106, Nichola, Rasit Ugur 107, Muhammet, Amar 108, Edouard, Falcone 109, Marco, Romain Santer 76, Frederic, Kalkanli 110, Arif, Kumar Karna 111, Keshab, 9 112 113, Kareim Khalafalla, B Vishwakarma 114, Ranjit, Finocchi 67, Federica, Giulioni 115, Carlo, Ceyhan 39, Erman, Çeker 116, Gökhan, Yazbeck 117, Chadi, Rajmil 118, Osvaldo, Yilmaz 119, Mehmet, Altay 120, Bari, L Barrett 121, Trenton, Seong Ngoo 122, Kay, Roychoudhury 123, Shubhadeep, Salvio 67, Gianmaria, Lin 124, Haocheng, Kadioglu 125, Ate, Timpano 109, Massimiliano, Avidor-Reiss 126 127, Tomer, Hakim 128, Lukman, Sindhwani 128, Puneet, Franco 129, Giorgio, Singh 130, Rajender, Giacone 131, Filippo, Ruzaev 132, Mikhail, Kosgi 133, Raghavender, Sofikitis 134, Nikolao, Palani 135, Ayad, Calik 87, Gokhan, Kulaksız 136, Deniz, Jezek 137, Davor, Al Hashmi 138 139, Manaf, Drakopoulos 140 141, Panagioti, Omran 142, Huda, Leonardi 143, Sofia, Celik-Ozenci 144, Ciler, Dokuzeylül Güngör 145, Nur, Ramsay 146, Jonathan, Amano 147, Toshiyasu, Sogutdelen 148, Emrullah, Wirya Kusuma Duarsa 149, Gede, Chiba 150, Koji, Jindal 151, Sunil, Savira 22, Missy, Boeri 152, Luca, Borges 153, Edson, Gupte 154, Deepak, Gokalp 155, Fatih, Hernández Hebrard 73, Guadalupe, Minhas 156, Suk, Shah 114, Rupin, and Andrology Forum, Global

Abstract

PURPOSE: Sperm DNA fragmentation (SDF) has been associated with male infertility and poor outcomes of assisted reproductive technology (ART). The purpose of this study was to investigate global practices related to the management of elevated SDF in infertile men, summarize the relevant professional society recommendations, and provide expert recommendations for managing this condition. MATERIALS AND METHODS: An online global survey on clinical practices related to SDF was disseminated to reproductive clinicians, according to the CHERRIES checklist criteria. Management protocols for various conditions associated with SDF were captured and compared to the relevant recommendations in professional society guidelines and the appropriate available evidence. Expert recommendations and consensus on the management of infertile men with elevated SDF were then formulated and adapted using the Delphi method. RESULTS: A total of 436 experts from 55 different countries submitted responses. As an initial approach, 79.1% of reproductive experts recommend lifestyle modifications for infertile men with elevated SDF, and 76.9% prescribe empiric antioxidants. Regarding antioxidant duration, 39.3% recommend 4-6 months and 38.1% recommend 3 months. For men with unexplained or idiopathic infertility, and couples experiencing recurrent miscarriages associated with elevated SDF, most respondents refer to ART 6 months after failure of conservative and empiric medical management. Infertile men with clinical varicocele, normal conventional semen parameters, and elevated SDF are offered varicocele repair immediately after diagnosis by 31.4%, and after failure of antioxidants and conservative measures by 40.9%. Sperm selection techniques and testicular sperm extraction are also management options for couples undergoing ART. For most questions, heterogenous practices were demonstrated. CONCLUSIONS: This paper presents the results of a large global survey on the management of infertile men with elevated SDF and reveals a lack of consensus among clinicians. Furthermore, it demonstrates the scarcity of professional society guidelines in this regard and attempts to highlight the relevant evidence. Expert recommendations are proposed to help guide clinicians.

Published
2023

5. Does Varicocele Repair Improve Conventional Semen Parameters? A Meta-Analytic Study of Before-After Data.

Author

Cannarella R, Shah R, Hamoda TAA, Boitrelle F, Saleh R, Gul M, Rambhatla A, Kavoussi P, Toprak T, Harraz AM, Ko E, Çeker G, Durairajanayagam D, Alkahidi N, Kuroda S, Crafa A, Henkel R, Salvio G, Hazir B, Darbandi M, Bendayan M, Darbandi S, Falcone M, Garrido N, Kosgi R, Sawaid Kaiyal R, Karna K, Phuoc NHV, Birowo P, Colpi GM, de la Rosette J, Pinggera GM, Nguyen Q, Zini A, Zohdy W, Singh R, Saini P, Glina S, Lin H, Mostafa T, Rojas-Cruz C, Arafa M, Calogero AE, Dimitriadis F, Kothari P, Karthikeyan VS, Okada K, Chiba K, Kadıoglu A, Altay B, Turunc T, Zilaitiene B, Gokalp F, Adamyan A, Katz D, Chung E, Mierzwa TC, Zylbersztejn DS, Paul GM, Sofikitis N, Sokolakis I, Malhotra V, Brodjonegoro SR, Adriansjah R, Tsujimura A, Amano T, Balercia G, Ziouziou I, Deswanto IA, Martinez M, Park HJ, Bakırcıoglu ME, Ceyhan E, Aydos K, Ramsay J, Minhas S, Al Hashimi M, Ghayda RA, Tadros N, Sindhwani P, Ho CCK, Rachman RI, Rodriguez Pena M, Motawi A, Ponnusamy AK, Dipankar S, Amir A, Binsaleh S, Serefoglu EC, Banthia R, Khalafalla K, Basukarno A, Bac NH, Singla K, Ambar RF, Makarounis K, Priyadarshi S, Duarsa GWK, Atmoko W, Jindal S, Arianto E, Akhavizadegan H, El Bardisi H, Shoshany O, Busetto GM, Moussa M, Jamali M, Al-Marhoon MS, Ruzaev M, Farsi HMA, Mutambirwa S, Lee DS, Kulaksiz D, Cheng YS, Bouzouita A, Sarikaya S, Kandil H, Tsampoukas G, Farkouh A, Bowa K, Savira M, Mogharabian N, Le TV, Harjanggi M, Anh DT, Long TQT, Soebadi MA, Hakim L, Tanic M, Ari UC, Parikh FR, Calik G, Kv V, Dorji G, Rezano A, Rajmil O, Tien DMB, Yuan Y, Lizarraga-Salas JF, Eze B, Ngoo KS, Lee J, Arslan U, and Agarwal A

Abstract

Purpose: The purpose of this meta-analysis is to study the impact of varicocele repair in the largest cohort of infertile males with clinical varicocele by including all available studies, with no language restrictions, comparing intra-person conventional semen parameters before and after the repair of varicoceles., Materials and Methods: The meta-analysis was performed according to PRISMA-P and MOOSE guidelines. A systematic search was performed in Scopus, PubMed, Cochrane, and Embase databases. Eligible studies were selected according to the PICOS model (Population: infertile male patients with clinical varicocele; Intervention: varicocele repair; Comparison: intra-person before-after varicocele repair; Outcome: conventional semen parameters; Study type: randomized controlled trials [RCTs], observational and case-control studies)., Results: Out of 1,632 screened abstracts, 351 articles (23 RCTs, 292 observational, and 36 case-control studies) were included in the quantitative analysis. The before-and-after analysis showed significant improvements in all semen parameters after varicocele repair (except sperm vitality); semen volume: standardized mean difference (SMD) 0.203, 95% CI: 0.129-0.278; p<0.001; I²=83.62%, Egger's p=0.3329; sperm concentration: SMD 1.590, 95% CI: 1.474-1.706; p<0.001; I²=97.86%, Egger's p<0.0001; total sperm count: SMD 1.824, 95% CI: 1.526-2.121; p<0.001; I²=97.88%, Egger's p=0.0063; total motile sperm count: SMD 1.643, 95% CI: 1.318-1.968; p<0.001; I²=98.65%, Egger's p=0.0003; progressive sperm motility: SMD 1.845, 95% CI: 1.537%-2.153%; p<0.001; I²=98.97%, Egger's p<0.0001; total sperm motility: SMD 1.613, 95% CI 1.467%-1.759%; p<0.001; l2=97.98%, Egger's p<0.001; sperm morphology: SMD 1.066, 95% CI 0.992%-1.211%; p<0.001; I²=97.87%, Egger's p=0.1864., Conclusions: The current meta-analysis is the largest to date using paired analysis on varicocele patients. In the current meta-analysis, almost all conventional semen parameters improved significantly following varicocele repair in infertile patients with clinical varicocele., Competing Interests: The authors have nothing to disclose., (Copyright © 2024 Korean Society for Sexual Medicine and Andrology.)

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results