1. Amphiphilic fluorescent probe self-encored in plasma to detect pH fluctuations in cancer cell membranes
- Author
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Sankarprasad Bhuniya, Kaustabh Kumar Maiti, Sanjib Samanta, Manu M. Joseph, Arup Podder, and Shayeri Biswas
- Subjects
Cell ,Tumor spheroid ,Fluorescence ,Catalysis ,Cell Line ,HeLa ,Surface-Active Agents ,Neoplasms ,Amphiphile ,Materials Chemistry ,medicine ,Humans ,Fluorescent Dyes ,Molecular Structure ,biology ,Chemistry ,Cell Membrane ,Metals and Alloys ,General Chemistry ,Plasma ,Hydrogen-Ion Concentration ,biology.organism_classification ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Membrane ,medicine.anatomical_structure ,Cancer cell ,Ceramics and Composites ,Biophysics - Abstract
We have developed an amphiphilic pH probe (P1CS) to detect pH levels in the plasma membrane in cancer cells. An elevated fluorescence signal at 550 nm at the cell surface of cancer cells (MDA-MB-231, HeLa cells) prompted the application of P1CS as a pH marker for the cancer cell surface, discriminating it from normal cells (WI-38). Moreover, the probe enables labeling of the surface of multilayered tumor spheroids, which promotes its use as a marker for the surface of tumor tissue.
- Published
- 2021
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