1. Evidence of Campylobacter jejuni reduction in broilers with early synbiotic administration
- Author
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Francesca Gaggia, Gabriella Di Serafino, Diana Di Gioia, Loredana Baffoni, Elisabetta Di Giannatale, Enrico Buglione, Giuliano Garofolo, Baffoni, Loredana, Gaggìa, Francesca, Garofolo, Giuliano, Di Serafino, Gabriella, Buglione, Enrico, Di Giannatale, Elisabetta, and Di Gioia, Diana
- Subjects
DNA, Bacterial ,0301 basic medicine ,Bifidobacterium longum ,030106 microbiology ,Oligosaccharides ,Glucuronates ,Synbiotics ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Campylobacter jejuni ,Microbiology ,Poultry ,law.invention ,Food safety ,Foodborne Diseases ,Caecum ,03 medical and health sciences ,Probiotic ,law ,Campylobacter Infections ,Xylooligosaccharide ,medicine ,Bifidobacterium longum PCB133 ,Animals ,Humans ,Food science ,DGGE ,Cecum ,Poultry Diseases ,Safety, Risk, Reliability and Quality. The EMIDA ERA‐NET is funded by the European Commission’s Seventh Framework Programme Contract No. 219235 ,Bifidobacterium ,2. Zero hunger ,biology ,Probiotics ,Campylobacter ,Broiler ,food and beverages ,General Medicine ,biology.organism_classification ,Molecular Typing ,030104 developmental biology ,Biological Control Agents ,Flock ,Infection ,Chickens ,Chicken microbiota ,Food Science - Abstract
C. jejuni is considered a food safety concern to both public health authorities and consumers since it is the leading bacterial cause of food-borne gastroenteritis in humans. A high incidence of C. jejuni in broiler flocks is often correlated to pathogen recovery in retail poultry meat, which is the main source of human infection. In this work broiler chickens were fed with a synbiotic product mixed with conventional feed using two different administration strategies. The synbiotic was formulated with the microencapsulated probiotic Bifidobacterium longum PCB133 and a xylo-oligosaccharide (XOS). 1-day old chicks were infected with C. jejuni strain M1 (105 cells) and the synbiotic mixture was then administered starting from the first and the 14th day of chicken life (for animal groups GrpC and GrpB respectively). The goal of this study was to monitor C. jejuni load at caecum level at different sampling time by real-time PCR, identifying the best administration strategy. The microbiological analysis of the caecal content also considered the quantification of Campylobacter spp., Bifidobacterium spp. and B. longum. The supplemented synbiotic was more successful in reducing C. jejuni and Campylobacter spp. when administered lifelong, compared to the shorter supplementation (GrpB). Bifidobacterium spp. quantification did not show significant differences among treatments and B. longum PCB133 was detected in both supplemented groups evidencing the successful colonization of the strain. Moreover, the samples of the control group (GrpA) and GrpC were analysed with PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to compare the caecal microbial community profiles at the beginning and at the end of the trial. Pattern analysis evidenced the strong influence of the early synbiotic supplementation, although a physiological change in the microbial community, occurring during growth, could be observed. Experimental results demonstrate that the synbiotic approach at farm level can be an effective strategy, combined with biosecurity measures, to improve the safety of poultry meat.
- Published
- 2017