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Evidence of Campylobacter jejuni reduction in broilers with early synbiotic administration
- Publication Year :
- 2017
-
Abstract
- C. jejuni is considered a food safety concern to both public health authorities and consumers since it is the leading bacterial cause of food-borne gastroenteritis in humans. A high incidence of C. jejuni in broiler flocks is often correlated to pathogen recovery in retail poultry meat, which is the main source of human infection. In this work broiler chickens were fed with a synbiotic product mixed with conventional feed using two different administration strategies. The synbiotic was formulated with the microencapsulated probiotic Bifidobacterium longum PCB133 and a xylo-oligosaccharide (XOS). 1-day old chicks were infected with C. jejuni strain M1 (105 cells) and the synbiotic mixture was then administered starting from the first and the 14th day of chicken life (for animal groups GrpC and GrpB respectively). The goal of this study was to monitor C. jejuni load at caecum level at different sampling time by real-time PCR, identifying the best administration strategy. The microbiological analysis of the caecal content also considered the quantification of Campylobacter spp., Bifidobacterium spp. and B. longum. The supplemented synbiotic was more successful in reducing C. jejuni and Campylobacter spp. when administered lifelong, compared to the shorter supplementation (GrpB). Bifidobacterium spp. quantification did not show significant differences among treatments and B. longum PCB133 was detected in both supplemented groups evidencing the successful colonization of the strain. Moreover, the samples of the control group (GrpA) and GrpC were analysed with PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to compare the caecal microbial community profiles at the beginning and at the end of the trial. Pattern analysis evidenced the strong influence of the early synbiotic supplementation, although a physiological change in the microbial community, occurring during growth, could be observed. Experimental results demonstrate that the synbiotic approach at farm level can be an effective strategy, combined with biosecurity measures, to improve the safety of poultry meat.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Bifidobacterium longum
030106 microbiology
Oligosaccharides
Glucuronates
Synbiotics
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Campylobacter jejuni
Microbiology
Poultry
law.invention
Food safety
Foodborne Diseases
Caecum
03 medical and health sciences
Probiotic
law
Campylobacter Infections
Xylooligosaccharide
medicine
Bifidobacterium longum PCB133
Animals
Humans
Food science
DGGE
Cecum
Poultry Diseases
Safety, Risk, Reliability and Quality. The EMIDA ERA‐NET is funded by the European Commission’s Seventh Framework Programme Contract No. 219235
Bifidobacterium
2. Zero hunger
biology
Probiotics
Campylobacter
Broiler
food and beverages
General Medicine
biology.organism_classification
Molecular Typing
030104 developmental biology
Biological Control Agents
Flock
Infection
Chickens
Chicken microbiota
Food Science
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....61708b896a82a105c7354651fa341b43