Search

Practical Application In this work, wine vinegars macerated with figs under different conditions have been characterized for the first time. Samples were analyzed by solid phase microextraction and gas chromatography coupled to mass spectrometry, sensory and physicochemical analysis. The results revealed that fig maceration of wine vinegars influenced the physicochemical, sensory, and volatile profiles of the final product, which were mainly affected by the fig amount (10, 20 or 30%), the fig type (fresh or dry) and the base wine vinegar (young or aged) used, and to a lesser extent the way the fig was added (crushed or chopped). The maceration, regardless the conditions used, increased the fruity notes, overall taste and visual impression, the tonality, total polyphenols and sugar content, and the presence of some volatile compounds, mainly terpenes and ketones, such as (Z)‐p‐mentha‐2,8‐dien‐1‐ol, or β‐damascenone. Therefore, maceration increased the organoleptic complexity, which could enhance the acceptability of consumers towards these products.This research studied how wine vinegars macerated with figs affect in different ways their taste, smell, and chemical properties. Thus, the results showed that these wine vinegars made with fig maceration by using different amounts and types of figs, as well as the type of vinegar used, changed their characteristics, making them smell and taste fruitier and more pleasant. This could be useful for making better‐tasting and more appealing vinegars for consumers who enjoy unique and enhanced flavors in their food. [ABSTRACT FROM AUTHOR]

The determination of optimal levels of green tea amount and brewing time would have a crucial role in the accumulation of desired aromatic volatile compounds to meet worldwide market demand. Aroma is the most important factor influencing tea consumers' choices along with taste, price, and brand. This study aims to determine how the brewing time and amount of green tea affect the aroma profile of green tea infusion. The effect of the amount of Turkish green tea (5–10 g) and brewing time (5–60 min) on aromatic volatile compounds was evaluated using solid‐phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS) technique. The SPME/GC–MS analysis identified 57 components in the aroma profile of green tea infusions including 13 esters, 12 alkanes, 7 unknowns, 6 ketones, 3 alcohols, 2 terpenes, 2 terpenoids, 1 alkaloid, 1 phenolic compound, 1 lactone, 1 pyrazine, and 1 norisoprenoid. The green tea amount and brewing time had significant effects on the number of chemical compounds. A total of 42, 47, and 36 aromatic volatile compounds were determined by brewing 5, 7.5, and 10 g of green tea. The most abundant constituents in green tea infusions were phytone, 2‐decenal, lauric acid, unknown 1, methoxy‐1‐methylethyl pyrazine, α‐ionone, β‐ionone, and diethyl phthalate (DEP). With this study, the aroma structures of green tea infusion have been revealed for the first time depending on the brewing time and quantity. [ABSTRACT FROM AUTHOR]

Coffee roasting is considered the most critical process in the development of sensory characteristics. During this stage, a substantial number of compounds are generated. Nevertheless, only a limited number of these compounds are responsible for the aroma, referred to as key aroma-active compounds. This study aimed to assess the impact of roasting levels on the formation of these compounds. Coffee was roasted at four different levels: very light (RL85), light (RL75), medium (RL55), and extremely dark (RL25), according to the Specialty Coffee Association (SCA) guidelines. The extraction, olfactory evaluation, and identification of compounds were performed using solid-phase microextraction (SPME), gas chromatography–olfactometry (GC-O), and gas chromatography–mass spectrometry (GC-MS), respectively. A total of 74 compounds were successfully identified, of which 25 were classified as aroma-active compounds. RL75 and RL85 displayed similar aromatic profiles. RL55 was characterized by pleasant notes such as sweet, toasted hazelnut, and caramel. In contrast, RL25 was marked by undesirable odors including burnt notes, putrid, and sulfurous scents. This study is the first to identify key odorant compounds in coffee based on SCA roasting standards. [ABSTRACT FROM AUTHOR]

While people use the aerial parts of some Sideritis species as tea and sweetener, some species are used for medicinal purposes. In this study, anti-urease, anti-xanthine oxidase, and antioxidant activity of Sideritis lanata was determined together with the essential oil contents. The essential oil compositions were determined solid phase micro-extraction by GC/MS. The IC50 value of the current study was found to be 41.420 µg/mL for inhibition of urease and 33.725 µg/mL for inhibition of xanthine oxidase. A total of 22 components were identified in the essential oil analysis. Spathulenol (24.63%) and Pentadecanolide (18.95%) in Sideritis lanata were identified as the most abundant essential oil compounds whereas Valeric acid (3.45%) and Palmitic acid (1.84%) were found as a major fatty acid. In the methanol extract of the investigated plant, the obtained total phenol content was 6.18 ± 0.42 mg of GAE/g. DPPH and FRAP values were determined as 61.44 ± 0.52 µmol FeSO4/g and 0.61 ± 0.01 mg/ml, respectively. This study is important because it is the first one, which reports the determination of anti-urease and anti-xanthine oxidase activity for Sideritis lanata. [ABSTRACT FROM AUTHOR]

An innovative SPME head space GC–MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations. [ABSTRACT FROM AUTHOR]

The aim of the entry was to write a substantial contribution that analyses and compares the biological pollution of indoor air, the possibilities of its assessment and the control methods. In addition, the aim of our entry was to review journals covering both commercial and residential buildings. By analysing the above topics from the existing articles, one can have the impression that air pollution is one of the most important problems that need to be solved in the modern world. Adequate air quality is important for maintaining human health, affects the health of ecosystems, including animals, and determines crop production. With the development of civilisation, the quality of air in the atmosphere and indoors is constantly deteriorating. Indoor air pollution can be divided into physical (e.g., noise, inadequate lighting, ionising radiation), chemical (e.g., tobacco smoke, household products) and microbiological (bacteria, viruses, fungi and products of their metabolism) factors. Each of these factors can have a negative impact on a person’s health or cause premature death. The entry deals with indoor air pollution, focussing on biological pollutants. It compares different methods available and describes the method of sampling to analyse indoor air pollution and ways to reduce it.

BACKGROUND RESULTS CONCLUSION The present study focuses on the aroma and secondary metabolites profiling of three Pelargonium graveolens cultivars, baladi (GRB), sondos (GRS) and shish (GRSH), grown in Egypt. Utilizing a multiplex approach combining high resolution‐ultraperformance liquid chromatography (HR‐UPLC)/tandem mass spectrometry (MS/MS) and gas chromatography (GC)‐MS coupled with chemometrics, the study aims to identify and profile various secondary metabolites and aroma compounds in these cultivars.HR‐UPLC/MS/MS analysis led to the annotation of 111 secondary metabolites, including phenolics, flavonoids, terpenes and fatty acids, with several compounds being reported for the first time in geranium. Multivariate data analysis identified vinylanisole, dimethoxy‐flavonol, and eicosadienoic acid as discriminatory metabolites among the cultivars, particularly distinguishing the GRS cultivar in its phenolics profile. In total, 34 aroma compounds were detected using headspace solid‐phase microextraction coupled with GC‐MS, including alcohols, esters, ketones, ethers and monoterpene hydrocarbons. The major metabolites contributing to aroma discrimination among the cultivars were β‐citronellol in GRB, α‐farnesene in GRS and isomenthone in GRSH.The study provides a comprehensive profiling of the secondary metabolites and aroma compounds in the three Pelargonium graveolens cultivars. The GRS cultivar was identified as particularly distinct in both its phenolics and aroma profiles, suggesting its potential as a premium variety for cultivation and use. Future studies should focus on isolating and investigating the newly detected metabolites and exploring the biological effects of these compounds in food applications and other uses. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Definition: The aim of the entry was to write a substantial contribution that analyses and compares the biological pollution of indoor air, the possibilities of its assessment and the control methods. In addition, the aim of our entry was to review journals covering both commercial and residential buildings. By analysing the above topics from the existing articles, one can have the impression that air pollution is one of the most important problems that need to be solved in the modern world. Adequate air quality is important for maintaining human health, affects the health of ecosystems, including animals, and determines crop production. With the development of civilisation, the quality of air in the atmosphere and indoors is constantly deteriorating. Indoor air pollution can be divided into physical (e.g., noise, inadequate lighting, ionising radiation), chemical (e.g., tobacco smoke, household products) and microbiological (bacteria, viruses, fungi and products of their metabolism) factors. Each of these factors can have a negative impact on a person's health or cause premature death. The entry deals with indoor air pollution, focussing on biological pollutants. It compares different methods available and describes the method of sampling to analyse indoor air pollution and ways to reduce it. [ABSTRACT FROM AUTHOR]

An array of analytical techniques (GC–MS, SPME–GC–MS, RP–HPLC–MS, FIA–MS, HPLC–UV, ICP–MS) was used to study the molecular composition of six ointment residues from the first third of the twentieth century. The objectives of the study were (i) to validate the applicability of a previously proposed strategy for the identification of lipids and active pharmaceutical ingredients in remains of historical pharmaceuticals and (ii) to shed light on early twentieth-century pharmaceutical practice. Although no further information on the composition of the samples studied was available (all were marked by a period pharmacist with the general label "Ointment" only), it was possible to identify the likely lipidic ointment bases as well as quantify the main likely active substances in all samples. This allowed us to partially reconstruct the composition of each ointment and to estimate its original purpose. [ABSTRACT FROM AUTHOR]

The chemical characterisation of archaeological finds necessitates a continuous focus on the development of nano/micro-invasive methodologies. These methodologies yield insights into the historical background, production processes, and conservation state of the items, often revealing astonishing properties of materials that have been unexpectedly preserved over the centuries. This work aims to characterise the molecular composition of a putative historical resin recovered during an archaeological excavation in Campese Bay off the island of Giglio in the 1980s. The resin was sampled from a trade amphora used for wine transport; it was likely employed as a coating to ensure better waterproofing of the containers. The characterisation was performed by detecting specific molecular markers using a GC–MS direct injection approach. In addition, we investigated the volatile fraction of the same sample through solid-phase microextraction (SPME) coupled with GC–MS to address the need for a fast, solvent-free, and field-compatible sample preparation method. [ABSTRACT FROM AUTHOR]

Lower urinary tract inflammation is a very common problem which occurs particularly in women. That is why the idea of a biotextronics system for preventive and supportive treatment came to be. The system is a kind of a therapeutic clothing in the form of underwear integrated with a four-layer pantiliner with biological active compounds (from chamomile essential oil) immobilized on the insert with a cellulose agar or microcrystalline cellulose agar film. In this research, the outer part of the insert was investigated for its ability to release compounds with antibacterial and anti-inflammatory activity under the temperature of the treatment (40 °C). The research was conducted on the day of the insert preparation (day 0) and also after 7, 14, 28, and 56 days to test the ability of the insert to be stored without changing its properties. The results showed that even after 56 days of storage, there are compounds released that are known to have antibacterial activity, such as α-bisabolol. The system requires further tests involving bacteria; however, chamomile essential oil seems to be good substrate for biotextronics systems for preventive and supportive treatment of lower urinary tract inflammations. [ABSTRACT FROM AUTHOR]

The major and possibly only component of the sex attractant pheromone of the moth Hemileuca nevadensis (Lepidoptera: Saturniidae) from southern California was determined to be (E10,Z12)-hexadecadienal (E10,Z12-16:Ald). Detectable quantities of the analogs (E10,Z12)-hexadecadien-1-yl acetate (E10,Z12-16:Ac) and (E10,Z12)-hexadecadien-1-ol (E10,Z12-16:OH) were also present in solvent extracts of sex pheromone glands, and stimulated male antennae in coupled gas chromatography-electroantennogram detector (GC-EAD) assays. GC-EAD traces from solid phase microextraction (SPME) wipe samples of sex pheromone glands of calling females confirmed the presence of E10,Z12-16:Ald and traces of E10,Z12-16:OH on the gland surface, but E10,Z12-16:Ac was not detected. Despite evidence for the presence of all three compounds in extracts, behavioral responses to synthetic compounds in the field suggested that only E10,Z12-16:Ald is required for optimal attraction. [ABSTRACT FROM AUTHOR]

Terpenes and terpenoids content in cannabis plant was already studied in the past with three used methods. Since these works did not compare the content of these substances under the same conditions, we tried to make this comparison exactly. Three different gas chromatography/mass spectrometry (GS/MS) methods—hexane-based liquid extraction (Lis), static headspace extraction (HS), and headspace solid-phase microextraction (SPME)—were compared to identify volatile compounds in four different cannabis chemotypes—Green fields chemotype, Titan chemotype, Black Domina chemotype, and Neptune chemotype. The main compounds focused on were monoterpenes/monoterpenoids and sesquiterpenes/sesquiterpenoids. For a final evaluation of the comparison of the three methods of analysis, hexane extraction gives comparable results (which is advantageous for quantitative analysis), although the other two methods allowed the identification of more substances. This means that the same method should be used everywhere for the quantitative evaluation of constituents in cannabis. [ABSTRACT FROM AUTHOR]

The increasing interest in hemp and cannabis poses new questions about the influence of drying and storage conditions on the overall aroma and cannabinoids profile of these products. Cannabis inflorescences are subjected to drying shortly after harvest and then to storage in different containers. These steps may cause a process of rapid deterioration with consequent changes in precious secondary metabolite content, negatively impacting on the product quality and potency. In this context, in this work, the investigation of the effects of freeze vs tray drying and three storage conditions on the preservation of cannabis compounds has been performed. A multi-trait approach, combining both solid-phase microextraction (SPME) two-dimensional gas chromatography coupled to mass spectrometry (SPME-GC × GC–MS) and high-performance liquid chromatography (HPLC), is presented for the first time. This approach has permitted to obtain the detailed characterisation of the whole cannabis matrix in terms of volatile compounds and cannabinoids. Moreover, multivariate statistical analyses were performed on the obtained data, helping to show that freeze drying conditions is useful to preserve cannabinoid content, preventing decarboxylation of acid cannabinoids, but leads to a loss of volatile compounds which are responsible for the cannabis aroma. Furthermore, among storage conditions, storage in glass bottle seems more beneficial for the retention of the initial VOC profile compared to open to air dry tray and closed high-density polyethylene box. However, the glass bottle storage condition causes formation of neutral cannabinoids at the expenses of the highly priced acid forms. This work will contribute to help define optimal storage conditions useful to produce highly valuable and high-quality products. [ABSTRACT FROM AUTHOR]

In recent years, on-site analysis has gained increasing attraction, aiming to minimize deviations caused by sample storage and transportation, as well as reduce delay time. Both sample pretreatment techniques and analytical instruments are crucial for on-site analysis. The technique of solid phase microextraction (SPME) offers benefits like solvent-free operation, high extraction efficiency, and miniaturization, making it a promising option for on-site sample pretreatment. Combining efficient analytical capability with on-site operational functionality, portable gas chromatography-mass spectrometry (GC–MS) enables rapid response during analysis. Their integration facilitates high-throughput detection of target analytes with improved sensitivity and stability. This review summarizes the advancements in portable GC–MS and SPME for on-site analysis in detail, providing new perspectives on challenges and future development.

The chemical industry explosion in the 20th century has led to increased environmental pollution, affecting fauna, flora, and waterways. These substances alter water's taste, color, and smell, making it unfit for consumption or toxic. Agricultural water networks face threats from pollution before and after treatment. Some chemical contaminants, like pesticides, are embedded in natural biogeochemical cycles. In this study, we developed a simple and low-cost procedure for the fabrication of needles coated with polydimethylsiloxane (PDMS) as an efficient sorbent for the microextraction of organic pollutant traces from water. The prepared needles were used as an alternative for commercial solid-phase micro-extraction (SPME) devices in analytical chemistry. The PDMS polymeric phase was characterized by Fourier-transform infrared spectroscopy (FT-IR), thermogravimetry (TGA), and scanning electron microscopy (SEM). The PDMS-coated needles were used for extraction of thirteen pesticides by direct-immersion solid-phase microextraction (DI-SPME) from contaminated waters, followed by determination with gas chromatography-mass spectrometry (GC-MS). The developed analytical method showed limits of detection (LODs) between 0.3 and 2.5 ng mL−1 and RSDs in the range of 0.8–12.2%. The homemade needles were applied for the extraction of pesticides in surface and ground aqueous samples collected from an agricultural area. Several target pesticides were identified and quantified in the investigated water samples. [ABSTRACT FROM AUTHOR]

The presence of methoxypyrazines in Cabernet Sauvignon wines produced from grapes grown in high-altitude and cold-climate regions is common and often treated as regional typicity. These compounds are considered a primary source of herbaceous and vegetal sensory characteristics, which may lead to unpleasant aromas when concentrations exceed the sensory threshold of these substances. Some strategies in the field and during winemaking have been studied with the aim of reducing the content of 2-methoxypyrazines. However, none describe the influence of spontaneous fermentation on the concentration of 2-methoxypyrazines. In this study, EMP (2-ethyl-5(6)-methoxypyrazine), IPMP (2-isopropyl-3-methoxypyrazine), SBMP (2-sec-butyl-3-methoxy-pyrazine), and IBMP (2-isobutyl-3-methoxy-pyrazine) were quantified in Cabernet Sauvignon wines produced via spontaneous fermentation and through fermentation with commercial yeasts. Grapes from vineyards located in the municipalities of Campo Belo do Sul (CBS) and São Joaquim (SJ), both in the state of Santa Catarina, Brazil, were used. Solid phase microextraction (SPME) coupled with the GCMS technique was used for the determination of the compounds. The primary methoxypyrazine found for both production sites was SBMP, with concentrations of 289.26 (CBS) and 681.55 (SJ) ng/L for spontaneous fermentation, and 619.58 (CBS) and 790.62 (SJ) ng/L for commercial yeast fermentation. Additionally, differences were found in other physicochemical quality analyses such as volatile acidity, anthocyanins, and total phenolics. The results indicate that spontaneous fermentation may be used as a strategy to reduce the content of methoxypyrazines in relation to what is present in the grape, particularly when harvest conditions do not allow for complete grape ripening. [ABSTRACT FROM AUTHOR]

In recent years, long periods of drought and heat waves have become increasingly frequent, causing forest dieback phenomena that make stands more sensitive to biotic stressors. How trees may respond to extreme climatic events and which metabolites are involved under stress conditions is still not clear. In this study, using Solid Phase Micro-Extraction (SPME)-GC/MS, we analysed how dieback (D) and non-dieback (ND) Hungarian oak trees from the San Paolo Albanese site respond to these climatic dynamics, focusing on volatile organic compounds (VOCs). For each group of trees, three wood samples were taken, and each was divided into four sub-samples with five growth rings and subjected to SPME and increase in basal area (BAI) analysis of the last 20 years. Dieback trees had a lower number of leaves, and this condition may translate into less photosynthesis, less organic matter production, and lower reserves of carbohydrates being available for growth. Indeed, D trees showed lower radial increases and a lower content of aldehydes, terpenes, and fatty acids than ND trees, indicating a better health of ND trees compared to D trees. Meanwhile, D trees showed a reduction in terpenes, such as α-pinene, γ-eudesmol, and cyperene (with significant insecticidal activity), a reduction in aromatic aldehydes, such as furfural and 5-methylfurfural, and an increase in silanols (with antimicrobial function). Considering the different compounds' contents between D and ND trees, our study could be useful for detecting bio-indicators to identify an early warning signal of dieback phenomena. [ABSTRACT FROM AUTHOR]

Saral Ö, Karaköse M. 2024. Determination of volatile oil compounds and antioxidant activities of some Cirsium taxa grown in Türkiye. Nusantara Bioscience 16: 62-67. Several Cirsium taxa are commonly used as a folk remedy or food in Anatolia and some countries. The study aims to determine the volatile oil profile and antioxidant activity of endemic Cirsium trachylepis Boiss., Cirsium echinus Hand.-Mazz., and Cirsium osseticum Petr. subsp. osseticum. The volatile oil compounds of three Cirsium species were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) using Solid Phase Microextraction (SPME). Additionally, total phenolic content was measured, and antioxidant activity capacity was determined by FRAP and DPPH• analyses in three Cirsium taxa methanolic extracts. A total of 32 (87.89%), 25 (88.69%), and 27 (80.68%) volatile compounds were identified from C. trachylepis, C. osseticum subsp. osseticum, and C. echinus, respectively. Pentadecanolide was the major volatile oil in three Cirsium species and was first reported in Cirsium taxa in this study. When the fatty acid content was examined, palmitic acid was determined as the common and main fatty acid for the three Cirsium species. A comparison of the antioxidant activity of three species showed that C. echinus had the highest antioxidant activity. The total phenolic content of C. echinus was found to be 250.84±0.46 mg GAE/100 g sample. While the FRAP value of C. echinus was 36.47±1.04 µmol Fe/g sample, the SC50 value was 1.89±0.05 mg/mL. This study may pave the way for the determination of volatile oils by SPME and the further development of research on Cirsium taxa. [ABSTRACT FROM AUTHOR]

The detection of explosives and explosive devices based on the volatile compounds they emit is a long-standing tool for law enforcement and physical security. Toward that end, solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) has become a crucial analytical tool for the identification of volatiles emitted by explosives. Previous SPME studies have identified many volatile compounds emitted by common explosive formulations that serve as the main charge in explosive devices. However, limited research has been conducted on initiators like fuses, detonating cords, and boosters. In this study, a variety of SPME fiber coatings (i.e., polydimethylsiloxane (PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), divinylbenzene/carboxin/polydimethylsiloxane (DVB/CAR/PDMS), and carboxin/polydimethylsiloxane (CAR/PDMS)) were employed for the extraction and analysis of volatiles from Composition C-4 (cyclohexanone, 2-ethyl-1-hexanol, and 2,3-dimethyl-2,3-dinitrobutane (DMNB)) and Red Dot double-base smokeless powder (nitroglycerine, phenylamine). The results revealed that a PDMS/DVB fiber was optimal. Then, an assortment of explosive items (i.e., detonation cord, safety fuse, slip-on booster, and shape charge) were analyzed with a PDMS/DVB fiber. A variety of volatile compounds were identified, including plasticizers (tributyl acetyl citrate, N-butylbenzenesulfonamide), taggants (DMNB), and degradation products (2-ethyl-1-hexanol). [ABSTRACT FROM AUTHOR]

Cadophora luteo‐olivacea is the causal agent of the skin‐pitting disease of kiwifruit, a syndrome that appears after 4–5 months of cold storage. However, it is assumed that the infection takes place in the field during fruit development. The present work takes into consideration the production of volatile organic compounds (VOCs) of Actinidia deliciosa 'Hayward' at different phenological phases as potential C. luteo‐olivacea infection biomarkers. In vitro assays were conducted to gain knowledge on the effect of kiwifruit VOCs on pathogen conidial germination and mycelial growth. VOCs produced by kiwifruit either inoculated or not with C. luteo‐olivacea were analysed at different phenological phases by SPME/GC–MS analysis. In particular, ethanol, o‐xylene, d‐limonene and acetic acid showed a significant increase in the presence of fungal inoculation. Ethanol and d‐limonene were also detected as volatile metabolites of the pathogen. The effect of each compound (ethanol, o‐xylene, d‐limonene and acetic acid) was tested on the fungal conidial germination at different concentrations, showing a growth stimulation at lower amounts. These results show how the production of some VOCs can contribute to the knowledge of fruit–pathogen interaction in the field with the aim of developing future tools for early disease detection and consequent effective control. [ABSTRACT FROM AUTHOR]

Transplant centers are currently facing a lack of tools to ensure adequate evaluation of the quality of the available organs, as well as a significant shortage of kidney donors. Therefore, efforts are being made to facilitate the effective use of available organs and expand the donor pool, particularly with expanded criteria donors. Fulfilling a need, we aim to present an innovative analytical method based on solid-phase microextraction (SPME) - chemical biopsy. In order to track changes affecting the organ throughout the entire transplant procedure, porcine kidneys were subjected to multiple samplings at various time points. The application of small-diameter SPME probes assured the minimal invasiveness of the procedure. Porcine model kidney autotransplantation was executed for the purpose of simulating two types of donor scenarios: donors with a beating heart (HBD) and donors after cardiac death (DCD). All renal grafts were exposed to continuous normothermic ex vivo perfusion. Following metabolomic and lipidomic profiling using high-performance liquid chromatography coupled to a mass spectrometer, we observed differences in the profiles of HBD and DCD kidneys. The alterations were predominantly related to energy and glucose metabolism, and differences in the levels of essential amino acids, purine nucleosides, lysophosphocholines, phosphoethanolamines, and triacylglycerols were noticed. Our results indicate the potential of implementing chemical biopsy in the evaluation of graft quality and monitoring of renal function during perfusion. [ABSTRACT FROM AUTHOR]

Solid-phase microextraction and comprehensive multidimensional gas chromatography represent two milestone innovations that occurred in the field of separation science in the 1990s. They have a common root in their introduction and have found a perfect coupling in their evolution and applications. This review will focus on food analysis, where the paradigm has changed significantly over time, moving from a targeted analysis, focusing on a limited number of analytes at the time, to a more holistic approach for assessing quality in a larger sense. Indeed, not only some major markers or contaminants are considered, but a large variety of compounds and their possible interaction, giving rise to the field of foodomics. In order to obtain such detailed information and to answer more sophisticated questions related to food quality and authenticity, the use of SPME-GC × GC–MS has become essential for the comprehensive analysis of volatile and semi-volatile analytes. This article provides a critical review of the various applications of SPME-GC × GC in food analysis, emphasizing the crucial role this coupling plays in this field. Additionally, this review dwells on the importance of appropriate data treatment to fully harness the results obtained to draw accurate and meaningful conclusions. [ABSTRACT FROM AUTHOR]

Bee bread is one of the least studied bee products. In this study, ten bee bread samples were characterized using palynology and HS–SPME–GC–MS (headspace solid-phase microextraction gas chromatography-mass spectrometry). In total, over one hundred different volatile components were identified, belonging to different chemical groups. Only ten common components were detected in all the samples. These volatiles were ethanol, methylene chloride, ethyl acetate, acetic acid, α-pinene, furfural, nonane, nonanal, n-hexane and isovaleric acid. Several other components were commonly shared among various bee bread samples. Over sixty detected compounds have not been previously reported in bee bread. The analysis required a mild extraction temperature of 40 °C, as higher temperatures resulted in the Maillard reaction, leading to the production of furfural. The profile of volatile compounds of the tested bee pollen samples was complex and varied. Some relationships have been shown between botanical origin and volatile organic compound profile. [ABSTRACT FROM AUTHOR]

Fifty-six samples of differently produced commercial Italian ciders were analysed for semi-volatile organic compounds (SVOCs) profiling, using comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GC×GC-TOF-MS) technique for the very first time. To properly support the compositional investigation of this emerging beverage, a chemometric approach through Principal Component Analysis (PCA) was employed. This revealed a sample distribution in agreement with results of the sensory tasting panel performed on such ciders, highlighting an excellent correlation between variables and perceived odorants. In particular, the positions of peculiar and anomalous objects in the Principal Components (PCs) space are explicitly evaluated, exploring the associated loadings (i.e., the importance of the identified chemical compounds), paying attention to their biochemical origin along the cider-making process and their impact on the sample olfactory analysis. Besides this, the t-distributed Stochastic Neighbor Embedding (t-SNE) method was shown to be an efficient tool for gathering pear ciders from the other samples (apple ciders), better than PCA. This study stands for the first survey on Italian commercial craft cider, and its results are aimed to be a milestone for its characterization and to start and promote cider culture in this country.

Despite growing decriminalization of Cannabis sativa (i.e., marijuana) possession throughout the United States of America, there remains to be an ongoing interest in the detection of unlawfully possessed and transported marijuana. This issue has resulted in an increasing interest regarding the generalization and specification related to the canine detection of marijuana. More specifically, canine trainers have expressed concerns on whether canines can generalize on the odor of marijuana regardless of the origin of their training materials. This research aims to differentiate multiple marijuana headspace samples from three regions in the USA based solely on the volatile organic compounds (VOCs) found in their odor profiles. In this study, a heated headspace solid phase micro-extraction (SPME) technique was optimized and implemented for the collection of both volatile terpenes and cannabinoids from marijuana. The headspace samples were analyzed using two full-scan, untargeted, optimized methods on a gas chromatograph coupled to a mass spectrometer (GC–MS), and a variety of chemometric tools were applied to the data to enable differentiation and potential classification between sample populations. Principal component analysis and sparse partial least squares discriminant analysis (sPLS-DA) employed in this study have demonstrated a disparity between marijuana varieties based on geography using the VOCs extracted from their odor profiles. With this research, it is intended to determine some fundamental differences between Cannabis of different geographical origins and set a foundation for the development and advancement of instrumental applications for other non-contact marijuana detection techniques in support of the improvement of illicit substance detection technology.

Species belonging to the genus Citrus are produced and traded in large amounts around the world. In addition to the consumption of citrus fruits as food due to their high vitamin C content, their use in many areas has become widespread with the development of the plant-based products industry. In this study, the amount and structure of volatile components of leaves and flowers of 5 different citrus species (Citrus aurantium, Citrus limon, Citrus paradisi, Citrus reticulata, and Citrus sinensis) were determined using the solid phase micro extraction. Monoterpene hydrocarbons and their oxygenated derivatives were identified as the most abundant chemical component groups. Limonene was the dominant compound in Citrus limon flowers (36.5%), leaves (22.5%) and Citrus paradisi flowers (22.4%). Linalool, and sabinene were the other major components. Linalool was determined at 50.5% in flowers and 73.3% in leaves of Citrus aurantium. Moreover, sabinene had a high amount in Citrus sinensis flowers (19.7%), leaves (24.7%), and in Citrus paradisi (27.4%) leaves. Apart from these dominant components, γ-terpinene (13.9%) and p-cymene (25.4%) were detected in Citrus reticulata flowers and leaves in an important amount. It was seen that the leaves and flowers of Citrus species gathered from Hatay province were an important source of limonene and linalool compounds.

Bacillus amyloliquefaciens (Ba) was applied to Mentha piperita L. (peppermint) seedlings grown at various salt levels (0, 50, 75, and 100 mM) for 42 days (six weeks). The study was conducted in a total of eight groups, with 24 seedlings per group. At the end of the study, the seedlings were analyzed for plant nutrient elements and volatile compound contents. The negative effect of salt was observed in almost all parameters. When all groups were evaluated for plant nutrient elements, Ba had a positive effect on Zn, Mn, Cu, and Na values compared to the control, but it did not show any effect on B, Fe, K, P, Mg, and Ca. In volatile compounds, limonene was detected as the major component in all groups. As a result of the evaluation based on limonene, the highest rate was found in the control, and the lowest rate was found in 100 mM NaCl. The salt-dependent inhibition between the groups with the highest and lowest limonene was 73%. While the negative effects of salt were observed in almost all parameters, the promoter effects of Ba were not as pronounced.

Abstract Bee bread is one of the least studied bee products. In this study, ten bee bread samples were characterized using palynology and HS–SPME–GC–MS (headspace solid-phase microextraction gas chromatography-mass spectrometry). In total, over one hundred different volatile components were identified, belonging to different chemical groups. Only ten common components were detected in all the samples. These volatiles were ethanol, methylene chloride, ethyl acetate, acetic acid, α-pinene, furfural, nonane, nonanal, n-hexane and isovaleric acid. Several other components were commonly shared among various bee bread samples. Over sixty detected compounds have not been previously reported in bee bread. The analysis required a mild extraction temperature of 40 °C, as higher temperatures resulted in the Maillard reaction, leading to the production of furfural. The profile of volatile compounds of the tested bee pollen samples was complex and varied. Some relationships have been shown between botanical origin and volatile organic compound profile.

An innovative SPME head space GC–MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations.

Coffee roasting is considered the most critical process in the development of sensory characteristics. During this stage, a substantial number of compounds are generated. Nevertheless, only a limited number of these compounds are responsible for the aroma, referred to as key aroma-active compounds. This study aimed to assess the impact of roasting levels on the formation of these compounds. Coffee was roasted at four different levels: very light (RL85), light (RL75), medium (RL55), and extremely dark (RL25), according to the Specialty Coffee Association (SCA) guidelines. The extraction, olfactory evaluation, and identification of compounds were performed using solid-phase microextraction (SPME), gas chromatography–olfactometry (GC-O), and gas chromatography–mass spectrometry (GC-MS), respectively. A total of 74 compounds were successfully identified, of which 25 were classified as aroma-active compounds. RL75 and RL85 displayed similar aromatic profiles. RL55 was characterized by pleasant notes such as sweet, toasted hazelnut, and caramel. In contrast, RL25 was marked by undesirable odors including burnt notes, putrid, and sulfurous scents. This study is the first to identify key odorant compounds in coffee based on SCA roasting standards.

This study focused on evaluating human body odor volatiles using a chamber approach. Ten participants were asked to sit inside the chamber for 1 h, while using SPME as the extraction technique for vapor sampling. A total of 105 compounds were detected across participants, with nonanal having the highest frequency. PCA statistical analysis depicted tighter clustering in female whole-body odor profiles when compared to males, thus corroborating gender odor differences. Concurrently, various biospecimens (hand, axillary, breath) from the same participants allowed for a comparison between whole-body and individual biospecimen odor signatures. When comparing whole-body sampling and distinctive biospecimens, nonanal and decanal were the only odor volatiles shared. Statistical clustering depicted higher similarity within the odor profiles of individual biospecimens compared to odor profiles of the whole body, indicating distinctiveness of the odor chemical landscape as a function of sampling region. Overall, this study demonstrated that SPME-GC/MS methodology was successful in the extraction, detection, and identification of previously reported human scent volatiles when employing the human chamber for whole-body sampling. Our presented testing paradigm allows for a direct comparison of odor volatiles across the full body and specific body locations that allows odor markers to be furthered exploited for diagnostic and biological detection contexts. [ABSTRACT FROM AUTHOR]

The phloem-feeder mealybug, Coccidohystrix insolita (Green, 1908) is a common herbivore which causes economic loss in crop yield every year in India. The present article is aimed at understanding the impact of mealybug infestation on metabolic system of eggplant (Solanum melongena L.). The alterations in the volatile profile have been investigated with the help of head-space-solid-phase-microextraction guided Gas Chromatography Mass spectrometry. Results showed occurrence of a reciprocal balance in total monoterpene, sesquiterpene and oxygenated monoterpene content for protection against the mealybug attack. A significant change in volatile scenario such as 4,8-dimethylnona-1,3,7-triene (4,8-DMNT), ß-ocimene, α-bergamotene and other compounds was observed. Some of these compounds belong to Green Leaf Volatile class to respond against biological stress. Quantities of secondary metabolites, like phenolic compounds was increased in damaged leaves. This preliminary finding highlighted the role of phytochemicals (both volatile and non-volatile) in defence mechanism of eggplant during herbivore attack in the tropical and subtropical climate of India. [ABSTRACT FROM AUTHOR]

Nanoparticle-based magnetic solid phase microextraction (MSPME) has advanced in heavy metal ion concentration and speciation in recent years. This comprehensive review covers the latest developments in this field and their application to complex food samples. The review begins with conventional MSPE methods' challenges and constraints, then examines off-line and online MSPE formats. Later sections of the review examine solid phase extraction's (SPE) use of magnetized inorganic nanomaterials. These include magnetic silica, alumina, titania, and layered double oxides. Magnetized carbonaceous nanomaterials, such as magnetic graphene and/or graphene oxides, carbon nanotubes, and carbon nitrides, also belong to this study. The study describes how magnetized organic polymers-non-imprinted and ion-imprinted improved SPE. Magnetized metal-organic frameworks (MOF), ionic liquids, and biosorbents are also covered briefly. Each section carefully examines nanomaterials' selectivity, sorption capacity, mechanisms of sorption, and synthesis routes. Nanomaterials are becoming key sorbents for toxic heavy metal extraction from food samples. Carbon nanomaterials (CNMs), magnetic nanoparticles (MNPs), nano-imprinted polymers (NIPs), nano-based metal-organic frameworks (N-MOFs), and silica nanoparticles (SiNPs) are leading preconcentration methods due to their high surface area, selectivity, rapid adsorption kinetics, and food contamination capture efficiency. The review emphasizes the importance of SPE and SPME, enhanced by nanomaterial sorbents and summarizes nanomaterialinfused solid phase extraction strategies and their impact on heavy metal extraction from food matrices. The review examines a variety of nanomaterials and their complex use to improve selectivity, extraction efficiency, and future research in this crucial area. [ABSTRACT FROM AUTHOR]

Damask rose (Rosa damascena Mill.) is an aromatic industrial plant with different applications. Selection of cultivars with high-value metabolites such as flavonoids—with acceptable yields—can lead to elite cultivars for mass propagation in various industries. A field experiment was carried out in a randomized complete block design (RCBD) to evaluate metabolites and some yield-related morphological data. In the present investigation, for the first time 13 flavonoid components of nine Iranian damask rose cultivars were compared using LC-MS/MS. As a result, 13 flavonoids were identified, most of which were reported for the first time in rose petals. Phloridzin (72.59–375.92 mg/100 g dw), diosmetin (82.48–153.16 mg/100 g dw) and biochanin A (0–1066.89 mg/100 g dw) were the most abundant, followed by trans-chalcone (0–106.29 mg/100 g dw) and diosmin (41.55–84.57 mg/100 g dw). Levels of naringenin also ranged from 3.77 in B111 to 54.70 mg/100 g dw in C294, while luteolin varied from 4.37 in B111 to 28.87 mg/100 g dw in C294. The SPME Arrow technique also was applied to determine the real aroma of the studied cultivars. Phenethyl alcohol was the most abundant compound, in the range of 69.28 to 77.58%. The highest citronellol/geraniol (C/G) was observed in D234 (4.52%) and D237 (4.30%), while the lowest amount belonged to A104 (1.28%). Rose oxide, as the most crucial factor for odor, ranged from 0.06% in D237 to 0.15% in D211. Based on cluster and principal component analysis (PCA), D234 cultivar can be suggested as a promising cultivar with high yield, high C/G content and high rose oxide, while D234 and C294 were the most valuable cultivars in terms of flavonoids with high yield. Finally, these cultivars can be introduced for further breeding programs and industrial cultivation. [ABSTRACT FROM AUTHOR]

A solid-phase microextraction (SPME) injection gas chromatography was validated with the flame ionization detection method (GC-FID) using a capillary column to detect ethanol. The method was used to determine ethanol in fluids with biomedical, clinical, and forensic importance, including water, phosphate-buffered saline (PBS), and artificial sweat. The strategy produced good peak resolution and showed a linear correlation between the concentration and peak areas for ethanol in all matrices. The inter- and intra-day precisions of the method were below 15.5% and 6.5%, respectively, varying according to the matrix. The method achieved detection limits below 1.3 mg/L, varying according to the matrix. Lower limits were obtained for the aqueous solution (0.22 mg/L), followed by the PBS solution (0.96 mg/L), and finally, the sweat solution (1.29 mg/L). This method is easy to perform and suitable for use in routine clinical biochemistry and forensic laboratories, allowing ethanol detection at lower concentrations using lower temperature and time of extraction, when compared with other studies. [ABSTRACT FROM AUTHOR]

Phthalates are the synthetic chemical plasticizers with the most varied uses and are a source of concern due to their toxicity and ubiquity, so much so that even plasticizer-free polymers can contain them as non-intentionally added substances (NIAS). Food packaging is among the materials with the greatest impact. In this study, a simple protocol is proposed for the location and identification of dimethyl phthalate, diethyl phthalate, dipropyl phthalate, and dibutyl phthalate which is applicable to compliance studies of food packaging materials and for the associated risk assessment. Solid phase microextraction gas chromatography/mass spectrometry was used to evaluate the migration of four NIAS from food packaging to release media simulating food substrates. Three plasticizer-free polymers were used: two that were lab-made and based on sodium alginate and a commercial polyethylene film. Linearity ranged from the LOQ to 10 µg/mL; within-day and between-day precision values were between 12.3–25.7% and 21.9–35.8%, respectively; the LOD and LOQ were in the range 0.029–0.073 µg/mL and 0.122–0.970 µg/mL. Migration tests were conducted for different periods of time at room temperature and at 8 °C. Exposure to microwaves (MW) was also evaluated. All packaging materials tested had global migration limits lower than 10 mg/dm2 of material surface. [ABSTRACT FROM AUTHOR]

The external appearance of some of the Protected Designation of Origin (PDO) cured cheeses is similar to other cheese samples made in Spain: 1 kg and 2.5–3 kg formats, cylindrical, and with or without a pleita mark on the surface. In this work, commercial cured ewe's milk cheese samples with a similar external appearance were analyzed, including five PDO and five non-PDO samples. The parameters analyzed were color, texture, pH, humidity, water activity, and the volatile profile. Additionally, a descriptive and consumer-sensory analysis of the cheese samples was carried out. Statistical analysis of the results showed that luminosity, color coordinates a* and b*, percentage of deformation, humidity, water activity, and acid contents were significantly higher in non-PDO cheese samples. The breaking force, maximum force, and the content of esters were significantly higher in those cheese samples with PDO. In addition, PDO cheese samples showed higher scores for all attributes evaluated by consumers, except for color. These results suggest that PDO cheeses are placed on the market with a higher degree of ripening than non-PDO ones and that consequently they are more positively valued by consumers. [ABSTRACT FROM AUTHOR]

The Eurasian Spruce Bark Beetle (Ips typographus) (L. 1758) (Coleoptera: Scolytinae) poses a significant threat to Eurasia's Norway spruce (Picea abies) (L.) Karst, forests. Early detection of infested trees is crucial to control beetle outbreaks and allow salvage logging before the next generation emerges. Besides traditional methods, new approaches focus on monitoring volatile organic compounds, mainly monoterpenes, emitted by infested trees. Using analytical chemistry, we studied the distribution of these compounds, particularly α-pinene, around infested trees. In lab trials, we optimized α-pinene detection using dynamic absorption and solid-phase microextraction (SPME), analyzed by gas chromatography with flame ionization detection (GC-FID). We conducted forest trials, revealing varying α-pinene abundance due to changing conditions. However, consistent trends emerged: levels were highest near the infested tree stem and 1.3 m above ground in the first trial and at a 1 m distance from the infested stem in the second. We generated a three-dimensional cloud depicting the distribution of α-pinene around infested trees in their natural habitat. These findings open avenues for detecting bark beetles on a large scale by mapping elevated concentrations of volatile organic compounds emitted by infested trees, potentially leading to alternative pest management methods. Scanning methods, such as electronic sensors combined with remote sensing, hold promise for this application. [ABSTRACT FROM AUTHOR]

Raisins, derived from dried grapes, represent a valuable commodity rich in secondary metabolites, particularly volatile organic compounds (VOCs). The primary objective of this review is to identify the VOCs that are influencing the aromatic profile of raisins to improve consumer preferences. However, extensive research has been done to optimize grape drying methods for different raisin attributes. In the context of this review, an in-depth investigation of published literature revealed the extraction of over 120 VOCs from raisins using SPME. Furthermore, we explored factors shaping raisin aroma and the sources of VOC generation. This review aims to pinpoint research gaps and provide an opportunity for future developments in studying raisins' aroma. This involves integrating advanced analytical techniques, examining processing method impacts, and considering consumer perception to enhance the overall understanding of raisin aromas. The outcomes are anticipated to provide valuable insights for the industry and the scientific community.

This paper represents an improved method for on-site quantitation of benzene, toluene, ethylbenzene, and xylenes (BTEX) in atmospheric air using solid-phase microextraction (SPME) and portable gas chromatography with a split/splitless inlet and photoionization detector (GC-PID). The developed method includes air pumping to four replicate 250 mL gas sampling bulbs, three of which are extracted by 85 µm Carboxen/polydimethylsiloxane (Car/PDMS) coating at 40°C, while the last one is spiked with BTEX standard in nitrogen and extracted under the same conditions. Compared to other similar SPME-based methods, the developed method does not require a standard gas generator and traceable certified permeation tubes for instrument calibration.The method was applied for on-site BTEX quantitation in air of Talgar, Almaty oblast, Kazakhstan in February and March 2024, at two different extraction times, among which 15 min was chosen as optimal because it provided sufficient detection limits of ethylbenzene, and xylenes. The on-site standard addition calibration allowed accurate BTEX quantitation. In February, only benzene (18.3 and 6.8 µg/m3) and toluene (15.7 and 8.5 µg/m3) were detected. In March samples, concentrations of benzene were 12.3 and 7.4 µg/m3, toluene - 23 and 5.4 µg/m3, ethylbenzene – 5.5 µg/m3 and not detected, m-/p-xylene - 3.6 and 2.1 µg/m3, o-xylene - 6.8 and 10.6 µg/m3. The LODs for benzene, toluene, ethylbenzene, m-/p-xylene, and o-xylene were 0.07, 0.3, 2, 0.8 and 2 µg/m3, respectively. The developed method can be recommended for quick and low-cost on-site quantification of BTEX in atmospheric air, particularly at remote locations.

IntroductionNormothermic ex vivo kidney perfusion (NEVKP) is designed to replicate physiological conditions to improve graft outcomes. A comparison of the impact of hypothermic and normothermic preservation techniques on graft quality was performed by lipidomic profiling using solid-phase microextraction (SPME) chemical biopsy as a minimally invasive sampling approach.MethodsDirect kidney sampling was conducted using SPME probes coated with a mixed-mode extraction phase in a porcine autotransplantation model of the renal donor after cardiac death, comparing three preservation methods: static cold storage (SCS), NEVKP, and hypothermic machine perfusion (HMP). The lipidomic analysis was done using ultra-high-performance liquid chromatography coupled with a Q-Exactive Focus Orbitrap mass spectrometer.ResultsChemometric analysis showed that the NEVLP group was separated from SCS and HMP groups. Further in-depth analyses indicated significantly (p < 0.05, VIP > 1) higher levels of acylcarnitines, phosphocholines, ether-linked and longer-chain phosphoethanolamines, triacylglycerols and most lysophosphocholines and lysophosphoethanolamines in the hypothermic preservation group. The results showed that the preservation temperature has a more significant impact on the lipidomic profile of the kidney than the preservation method’s mechanical characteristics.ConclusionHigher levels of lipids detected in the hypothermic preservation group may be related to ischemia-reperfusion injury, mitochondrial dysfunction, pro-inflammatory effect, and oxidative stress. Obtained results suggest the NEVKP method’s beneficial effect on graft function and confirm that SPME chemical biopsy enables low-invasive and repeated sampling of the same tissue, allowing tracking alterations in the graft throughout the entire transplantation procedure.

Terpenes and terpenoids content in cannabis plant was already studied in the past with three used methods. Since these works did not compare the content of these substances under the same conditions, we tried to make this comparison exactly. Three different gas chromatography/mass spectrometry (GS/MS) methods—hexane-based liquid extraction (Lis), static headspace extraction (HS), and headspace solid-phase microextraction (SPME)—were compared to identify volatile compounds in four different cannabis chemotypes—Green fields chemotype, Titan chemotype, Black Domina chemotype, and Neptune chemotype. The main compounds focused on were monoterpenes/monoterpenoids and sesquiterpenes/sesquiterpenoids. For a final evaluation of the comparison of the three methods of analysis, hexane extraction gives comparable results (which is advantageous for quantitative analysis), although the other two methods allowed the identification of more substances. This means that the same method should be used everywhere for the quantitative evaluation of constituents in cannabis.

The development of techniques for detecting halal and non-halal animal meat is of great importance as a useful tool for monitoring and assuring food quality for Muslim. Flavor or aroma is one of the sensory attributes for consumers to distinguish broth cattle. The differences aroma of broth cattle is contributed by the content of volatile compounds. This study was aimed to determine the composition of volatile compounds in meat and bone broth from five different animals, i.e., cow (beef), pig (pork), goat, lamb, and chicken for initial identification of halal and non halal food. A preliminary test for samples of meat and bone broth was done by identifying their physical properties including pH, viscosity, and density. The volatile compounds in meat and broth samples were extracted using a solid phase-micro extraction (SPME) at room temperature and analyzed by gas chromatography-mass spectrometry (GCMS). The results revealed the physical properties of meat and bone broth, i.e., pH, viscosity, and density, cannot be used to distinguish the type of broth produced from beef, pork, goat, lamb, and chicken. Successful results for the identification were achieved through chromatogram profile of volatile compounds from meat and bone broth which show characteristics to each type of animal, and therefore it can be used to distinguish pork from beef, goat, lamb, and chicken as initial detection for halal and non halal food. The research also identified 7 predominant volatile compounds as a marker for meat pork and other 8 specific compounds marker for pork bone.

Solid-phase microextraction (SPME) via on-fiber derivatization with O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine (PFBHA) and gas chromatographic determination is considered a technique of choice in many analytical fields for formaldehyde (FA) monitoring. Vapor phase adsorption models of experimentally loaded PFBHA on porous divinylbenzene (DVB) SPME were investigated at 60°C, 35 cm s−1 of air velocity, in a 1-64 min range: with the fiber completely exposed, loaded PFBHA was about 276 μg. Among the models tested, i.e. heat transfer, pseudo-second-order (PSO), Elovich, intra-particle diffusion, extra-particle diffusion and Langmuir, PFBHA adsorption was best fit by the PSO model, showing agreement with experimental data (272 μg). The sampling rate of FA in our conditions, obtained with a permeation tube system, was in agreement with literature (17.4 and 18.3 mL min−1, respectively). Thus, an overall standardization of the sampling phase is presented, leaving the sampling time as the most crucial parameter to be set for future applications.

Main pharmacological activities observed with the aromatic herbs celery, coriander, dill, and fennel have been attributed primarily to their essential oils, obtained by hydro-distillation. Still, there is a lack in research on the flavor of these selected four aromatic plants with multiple biological activities. The current study sought to identify and quantify the chemical composition of the spontaneous emitted volatile organic compounds (VOCs) of their fresh leaves, and to statistically analyze their composition using principal component analysis (PCA) and hierarchial cluster analysis (HCA). In vitro cytotoxic activities of their hydro-distilled essential oils were evaluated against MCF-7 and HCT-116 cell lines using MTT assay. The emitted VOCs, captured by solid phase micro extraction (SPME) method were analyzed by GC/GC-MS. The main terpenoid components of the aroma profile of celery and coriander were limonene, that of dill α-phellandrene, and of fennel α-terpinene. The systematic analysis using both, HCA and PCA of the spontaneous emitted VOCs of the fresh leaves of the four Apiaceae members growing in Jordan, revealed a clear picture of the distinct odor of these edible aromatic plants. The hydro-distilled essential oil of fennel exhibited the best antiproliferative activity against MCF-7 and HCT-116 cell lines with respective IC50's of 0.35, and 0.42 mg/mL. The composition of the VOCs of the leaves of celery, coriander, dill, and fennel, growing in Jordan was determined for the first time, in addition to their antiproliferative activities. [ABSTRACT FROM AUTHOR]

In order to explore the release characteristics of volatile components of flowers of Dendrobium, screen valuable germplasm resources of the Dendrobium orchids, and provide a theoretical basis for the breeding for fragrant flower varieties of Dendrobium, in this study, using Dendrobium primulinum, Dendrobium loddigesii, Dendrobium parishii, and Dendrobium Red Dragon as materials，the volatile components and their relative contents in the flowers of four types of fragrant Dendrobium were determined by solid-phase microextraction(SPME) combined with GC-MS. The results showed that 72 volatile components were identified in the flowers of 4 species of Dendrobium in full flowering stage, the composition and content of the volatile components were significantly different. The main volatile components of D. primulinum was Propylcyclopentane(7.65 μg/g) followed by Benzyl acetate(1.68 μg/g). The content of Cyclooctane in the volatile components of D. primulinum was the highest(124.52 μg/g), followed by(+)-3-Carene(2.39 μg/g). The main and secondary volatile components of D. parishii and D. Red Dragon were the same, they were 2-Pentadecanone(51.47 μg/g, 11.77 μg/g) and 2-Tridecanone(1.69 μg/g, 1.79 μg/g). [ABSTRACT FROM AUTHOR]