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Diethylene triamine trinitrate (DETN) (100% pure) was tested for its potential to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activiation according to OECD TG 473 in compliance with Good Laboratory Practice. DETN was found negative at concentrations 100, 500, 100, 2500, and 5000 ug/mL in both with and without activation. A confirmatory chromosome aberration assay was performed without activation also showed negative.

Ethylenediamine dinitrate (EDDN) (99.5% pure) was tested for clastogenic potential to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice,. EDDN was tested at concentrations 500, 1000, 2000, 3000, 4000 and 5000 ug/mL both with and without activation. Based on the cytotoxicity data, chromosome aberrations were scored from the cells treated with the concentrations 1000, 2000, and 3000 ug/mL without activation and 3000, 4000, and 5000 ug/mL with activation. Two hundred metaphases were scored from each concentration and controls. EDDN did not induce a statistically significant increase in the percentage of chromosome aberration, polyploidy and endoreduplicated cells in the CHO cells both with and without activation. A confirmatory chromosome aberration assay was performed without activation also showed negative.

Diethylene triamine trinitrate (DETN) (100% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. The results showed that DETN was not mutagenic in Salmonella typhimurium and Escherichia coli both with and without activation at concentration of 100, 500, 1000, 3000, and 5000 ug/plate with and without activation.

Diethylene triamine trinitrate (DETN) (100%) pure) was tested for its potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and female CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 500, 1000, and 2000 mg;/kg. Animals were euthanized approximately 24 and 48 hr after dosing. The percent of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hrs. Cytotoxicity was assessed by scoring the number of PCE and norchromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were no statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicated that DETN was negative in vivo mouse micronucleus assay.

Ethylenediamine dinitrate (EDDN) (99.5% pure) was tested forclastogenic potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and femal CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 500, 1000, and 2000 mg/kg. Animals were euthanized approximately 24 and 48 hr after dosing. The percentage of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hours. Cytotoxicity was assessed by scoring the number of PCE and norchromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were not statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicate that EDDN was negative in the in vivo mouse micronucleus assay and therefore is not considered to be a clastogenic agent.

Ethylenediamine dinitrate (EDDN) (99.5% pure) was tested for mutagenic potential with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice, EDDN was tested at doses 100, 500, 1000, 3000 ug/plate and 5000 ug/plate. The results showed that EDDN was not mutagenic in Salmonella typhimurium and Escherichia coli both with and without activation in tster strains (TA 98, TA 1535, and TA 1537) but it showed positive increase in TA 100 tester stain without activation (3.1-fold) at 5000 ug/plate. This was also verified with confirmatory mutation assay at concentrations up to 5000 ug/plate with activation and also found positive (2.5 fold) in TA 100. No positive increases were observed with other tester strains (TA 98, TA 1535, TA 1537) with or without activation. Based on the positive response was only in one strain TA 100 observed at the highest dose under conditions oftest and according to criteria set for evaluating test results, the EDDN was weak positive in this system both with and without activation.

N,N,N',N'-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.

N,N,N(prime),N(prime)-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and female CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 62.5,125 and 250 mg/kg. Animals were euthanized approximately 24 and 48 hrs after dosing. The percent of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hrs. Cytotoxicity was assessed by scoring the number of PCE and normochromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were no statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicate that TMEDA was negative in the in vivo mouse micronucleus assay and the compound was not clastogenic.

3-Nitro-1,2,4-triazol-5-one (NTO, 99.6% pure) was tested for its potentials to induce mutation at the thymidine kinase locus of L5178Y TK +/- mouse lymphoma cells in vitro with and without metabolic activation according to OECD TG 476 in compliance with Good Laboratory Practice. Cell cultures were treated with NTO at concentrations of 10, 50, 100, 250, 500, 1000, 2500, and 5000 with and without activation and were found negative in the mutation assay. A confirmatory mutation assay with 24 hrs treatment period and without activation was performed by using the same concentrations of NTO. These results were also negative.

N,N,N',N'-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 750, 1,000, 3,000 and 5,000 g/plate for salmonella strains and Escherichia coli without and with activation. The results showed that TMEDA was not mutagenic in these strains both with and without activation. The result of confirmatory mutation assay with and without activation also showed negative response. TMEDA was negative in the Salmonella typhimurium/Escherichia coli plate incorporation mutation assay both with and without activation.

Dimethylamine-2-2ethyl azide (DMAZ, 99.3% pure) was tested for its potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and female CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 125, 250, and 500 mg/kg. Animals were euthanized approximately 24 and 48 hrs after dosing. The percentage of polychromatic erythrocytes (PCE) and the frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hrs. Cytotoxicity was assessed by scoring the number of PCE and norchromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were no statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicate that DMAZ did not cause chromosome damage in the in vivo mouse micronucleus assay and therefore it is not considered to be a clastogenic agent.

Dimethylamine-2-2ethyl azide (DMAZ, 99.3% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. DMAZ was evaluated at concentrations of 10, 50, 100, 500, 1000, 2500, and 5000 microg/mL in both with and without activation and was found negative for clastogenic potential. A confirmatory chromosome aberration assay was performed without activation also showed negative.

3-Nitro-1,2,4-triazol-5-one (NTO, 99.6% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. NTO was tested at concentrations of 5, 10, 50, 100 and 250 g/plate for Salmonella strains and 100, 250, 500, 750 and 1000 g/plate for Escherichia coli without activation. The dose levels were 100, 500, 1000, 2500 and 5000 g/plate for both Salmonella and Escherichia coli with activation. The results showed that NTO was not mutagenic in Salmonella typhimurium and Escherichia coli both with and without activation. The negative result was also verified by a confirmatory mutation assay both with and without activation.

3-Nitro-1,2,4-triazol-5-one (NTO, 99.6% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. NTO was found negative at concentrations of 5, 50, 100, 500, 1000, 2500, and 5000 g/mL in both with and without activation. A confirmatory chromosome aberration assay was performed without activation also showed negative.