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3,423 results on '"SFR Condorcet"'

1. Virtual decoupling to break the simplification versus resolution trade-off in nuclear magnetic resonance of complex metabolic mixtures

Author: Alain Geffard, Jean-Marc Nuzillard, Guy Lippens, Neil Cox, Cyril Charlier, Burkhard Luy, Sophie M. Prud’homme, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Karlsruhe Institute of Technology (KIT), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet
Subjects: Physics, QC501-766, Proton, 010405 organic chemistry, Resolution (electron density), Spectrum (functional analysis), Decoupling (cosmology), 010402 general chemistry, 01 natural sciences, Spectral line, 0104 chemical sciences, Electricity and magnetism, Nuclear magnetic resonance, Dimension (vector space), [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Heteronuclear single quantum coherence spectroscopy
Abstract: The heteronuclear single quantum correlation (HSQC) experiment developed by Bodenhausen and Ruben (1980) in the early days of modern nuclear magnetic resonance (NMR) is without a doubt one of the most widely used experiments, with applications in almost every aspect of NMR including metabolomics. Acquiring this experiment, however, always implies a trade-off: simplification versus resolution. Here, we present a method that artificially lifts this barrier and demonstrate its application towards metabolite identification in a complex mixture. Based on the measurement of clean in-phase and clean anti-phase (CLIP/CLAP) HSQC spectra (Enthart et al., 2008), we construct a virtually decoupled HSQC (vd-HSQC) spectrum that maintains the highest possible resolution in the proton dimension. Combining this vd-HSQC spectrum with a J-resolved spectrum (Pell and Keeler, 2007) provides useful information for the one-dimensional proton spectrum assignment and for the identification of metabolites in Dreissena polymorpha (Prud'homme et al., 2020).
Published: 2021

2. Development of an exposure protocol for toxicity test (FEET) for a marine species: the European sea bass (Dicentrarchus labrax)

Author: Christelle Jozet-Alves, Sofia Soloperto, Christophe Minier, Salima Aroua, Marie-Pierre Halm-Lemeille, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Ethologie animale et humaine (EthoS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), FR 3473 Institut universitaire Mer et Littoral (IUML), Le Mans Université (UM)-Université d'Angers (UA)-Université de Nantes (UN)-École Centrale de Nantes (ECN)-Université de Bretagne Sud (UBS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Normandie Region Region Normandie, urban community Le Havre Seine Metropole, IFREMER institute (program PENELOP), Research Federation FR CNRS [3730], European Union European Commission, urban community LHSM (Le Havre Seine Metropole), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 4-Dichloroaniline, Health, Toxicology and Mutagenesis, Behavioral test, Zoology, Temperate conditions, Context (language use), 010501 environmental sciences, Biology, Test (biology), 01 natural sciences, Marine species, Lethal Dose 50, 96 h LC50, Early-life stage toxicity test, Toxicity Tests, Environmental Chemistry, Ecotoxicology, Animals, 14. Life underwater, Sea bass, 0105 earth and related environmental sciences, Hatching, 010604 marine biology & hydrobiology, General Medicine, biology.organism_classification, Pollution, 3 4-Dichloroaniline, Larva, Toxicity, Sea bass larvae, Dicentrarchus, Bass, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract: International audience; Regulatory assessment of the effects of chemicals requires the availability of validated tests representing different environments and organisms. In this context, developing new tests is particularly needed for marine species from temperate environments. It is also important to evaluate effects that are generally poorly characterized and seldom included in regulatory tests. In this study, we designed an exposure protocol using European sea bass (Dicentrarchus labrax) larvae. We examined classical toxicological values (LCx) as well as behavioral responses. By comparing different hatching and breeding strategies, we defined the optimal conditions of exposure as non-agitated conditions in 24- or 48-well microplates. Our exposure protocol was then tested with 3,4-dichloroaniline (3,4-DCA), a recommended reference molecule. Based on our results, the 96 h LC50 for 3,4-DCA corresponded to 2.04 mg/L while the 168 h LC50 to 0.79 mg/L. Behavioral analyses showed no effect of 3,4-DCA at low concentration (0.25 mg/L). In conclusion, the present work established the basis for a new test which includes behavioral analysis and shows that the use of sea bass is suitable to early-life stage toxicity tests.
Published: 2022

3. Woody Plant Declines. What’s Wrong with the Microbiome?

Author: Sophie Trouvelot, Olivier Fernandez, Florence Fontaine, Pierre-Emmanuel Courty, Pauline Bettenfeld, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], and Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0106 biological sciences, 0301 basic medicine, Nitrogen, ecological engineering, [SDV]Life Sciences [q-bio], microbiome, Plant Science, Biology, 01 natural sciences, woody plant decline, 03 medical and health sciences, homeostasis, Microbiome, Soil Microbiology, ComputingMilieux_MISCELLANEOUS, holobiont, 2. Zero hunger, Ecology, Microbiota, Plants, 15. Life on land, Economic reality, Ecological engineering, Carbon, Holobiont, 030104 developmental biology, [SDE]Environmental Sciences, rhizosphere, 010606 plant biology & botany, Woody plant
Abstract: National audience; Woody plant (WP) declines have multifactorial determinants as well as a biological and economic reality. The vascular system of WPs involved in the transport of carbon, nitrogen, and water from sources to sinks has a seasonal activity, which places it at a central position for mediating plant–environment interactions from nutrient cycling to community assembly and for regulating a variety of processes. To limit effects and to fight against declines, we propose: (i) to consider the WP and its associated microbiota as an holobiont and as a set of functions; (ii) to consider simultaneously, without looking at what comes first, the physiological or pathogenic disorders; and (iii) to define pragmatic strategies, including preventive and curative agronomical practices based on microbiota engineering.
Published: 2020

4. Hygrothermal evaluation of hemp concrete at wall and room scales: Impact of hysteresis and temperature dependency of sorption curves

Author: Guillaume Polidori, Tala Moussa, Chadi Maalouf, Elias Kinab, Georges Costantine, Groupe de Recherche en Sciences Pour l'Ingénieur - EA 4694 (GRESPI), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [PHYS]Physics [physics], Dependency (UML), 020209 energy, Global warming, 0211 other engineering and technologies, Context (language use), Sorption, 02 engineering and technology, Building and Construction, Energy consumption, 7. Clean energy, Hemp concrete, Hysteresis (economics), 13. Climate action, 021105 building & construction, Sustainability, 0202 electrical engineering, electronic engineering, information engineering, Environmental science, General Materials Science, Geotechnical engineering
Abstract: International audience; In the context of global warming associated with the excess of energy consumption, energy saving and environmental sustainability in buildings have become significant issues at both national and European levels. Therefore, the adoption of energy-efficiency measures in new and renovated dwellings will increase the demand for thermal insulation, which is crucial to improve the housing comfort by reducing the heat loss through the building envelope. Over the past two decades, several bio-based materials have been used in building thermal insulation, such as hemp concrete. Promoting the use of hemp concrete in new constructions and restoration of existing buildings first requires a validation of its hygrothermal behavior at both wall and building scales. For this, several models dealing with various physical aspects of hemp concrete were developed to understand their impact on the prediction of changes in temperature and relative humidity. These physical phenomena include hemp concrete hygroscopic hysteresis and thermal dependency of sorption curves. The preliminary results show that hysteresis has a significant influence on the numerical prediction in the short-term, while temperature dependency has less influence at the wall scale. The next part of this article is devoted to the study of these phenomena at the room scale by monitoring the internal conditions and energy consumption of a virtual hemp concrete office. The results highlight the fact that hysteresis and thermal dependency have low impact on indoor conditions, which could probably be related to the other parameters at the room scale.
Published: 2020

5. Synthesis and characterization of polymethine dyes carrying thiobarbituric and carboxylic acid moieties

Author: Arthur Desvals, Mariagrazia Fortino, Corentin Lefebvre, Johann Rogier, Clément Michelin, Samy Alioui, Elodie Rousset, Alfonso Pedone, Gilles Lemercier, Norbert Hoffmann, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Modena e Reggio Emilia (UNIMORE), Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE)
Subjects: [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, polymethine dyes, Materials Chemistry, [CHIM]Chemical Sciences, General Chemistry, DFT, Catalysis, Spectroscopy, DFT, polymethine dyes
Abstract: International audience; An efficient synthesis of polymethine dyes carrying thiobarbituric and a carboxylic acid moiety has been developed. Such compounds play a key role in many photometric detections and quantifications of enzyme activities. In such tests, the metabolite of the enzyme activities is transformed into a β-dicarbonyl derivative. In the present study, this compound was prepared from furfural through organic synthesis. Its in situ transformation with thiobarbituric acid derivatives yields the target compounds on a gram-scale (0.4 to 0.6 g). A combined experimental and theoretical study of the photophysical properties of the synthesized compounds was carried out. Absorption and emission spectroscopy measurements highlighted a slight solvatochromism effect. The luminescence was quenched by molecular oxygen, indicating the partial triplet multiplicity character of the lowest excited state. Density Functional Theory (DFT) calculations have been applied for the evaluation of favoured conformations for these new compounds and the study of their optical properties. Within the Franck Condon principle, vibrationally resolved electronic one-photon absorption spectrum has been simulated. This simulation shows the presence of a major band followed by a vibronic sideband, typical of organic chromophores in solution. The performed computational study revealed that the transition from the ground to the first excited electronic state has a π-π* character. Finally, TD-DFT energy level diagram calculations highlighted the presence of triplet states very close to the first singlet excited one, suggesting probable access to the triplet-excited state.
Published: 2022

6. Photocatalytic Radical Addition to Levoglucosenone

Author: Corentin Lefebvre, Terence Van Gysel, Clément Michelin, Elodie Rousset, Djibril Djiré, Florent Allais, Norbert Hoffmann, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA), Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Agro-Biotechnologies Industrielles (ABI), AgroParisTech, Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: radical reactions, 010405 organic chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, asymmetric synthesis, Organic Chemistry, [CHIM.CATA]Chemical Sciences/Catalysis, 010402 general chemistry, renewable resources, 01 natural sciences, 0104 chemical sciences, 3. Good health, polyoxometalate, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, photocatalysis
Abstract: International audience; Using photocatalysis with tetra-n-butylammonium decatungstate (TBADT), alkanes, cyclic acetals, cyclic ethers, formamide and aldehydes were added in a stereoselective way to levoglucosenone (LGO). A hydrogen atom is transferred from the donor compound to the photochemically excited TBADT, and the resulting radicals add onto LGO in a stereoselective way. In the case of the addition of adamantane, two regioisomers were obtained which form a crystalline solid solution. Cyrene™, obtained by hydrogenation of LGO, was added under the same conditions. In this case, only two of 32 possible isomers of the resulting Cyrene™ dimer were formed. The regio-selectivity of the HAT step is discussed in detail. For this purpose, bond dissociation energies and partial charges have been calculated. Transition state calculations of the radical addition to LGO explain the stereospecificity of this reaction step.
Published: 2021

7. Generation-recombination in disordered organic semiconductor: Application to the characterization of traps

Author: Ndèye Saly Ndiaye, O. Simonetti, Thien-Phap Nguyen, Louis Giraudet, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), European Community through the FEDER project POLCAEstonian Research CouncilEuropean CommissionRegion Grand-EstFEDER funds from the European Community, European Project: 610686,EC:FP7:ICT,FP7-ICT-2013-10,POLCA(2013), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), and Université de Nantes (UN)-Université de Nantes (UN)
Subjects: Condensed Matter::Quantum Gases, Deep-level transient spectroscopy, Materials science, 02 engineering and technology, General Chemistry, Activation energy, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, Molecular physics, Stability (probability), Arrhenius plot, Electronic, Optical and Magnetic Materials, Biomaterials, Trap (computing), Organic semiconductor, 0103 physical sciences, Materials Chemistry, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Electrical and Electronic Engineering, 010306 general physics, 0210 nano-technology, HOMO/LUMO, Diode
Abstract: International audience; The presence of traps in organic semiconductor based electronic devices affects considerably their performances and their stability. The Shockley-Read-Hall (SRH) model is generally used to extract the trap parameters from the experimental results. In this paper, we propose to adapt the SRH formalism to disordered organic semiconductors by considering a hopping transport process and Gaussian distributions for both mobile and trapped carriers. The model is used to extract multiple trap parameters from charge based Deep Level Transient Spectroscopy (QDLTS) spectrum. Calculation of the charge transients are given in detail. The model predicts that the activation energy of the trap should not follow an Arrhenius plot on large temperature ranges. Also, the charge transients are no longer exponential when considering Gaussian trap distributions, enlarging the Q-DLTS peaks. The model fits the Q-DLTS spectra measured on organic diodes with a limited number of trap contributions with a good agreement. It is found that an increase of the material rate of disorder reduces the extracted trap energy distances to the LUMO but has no influence on the extracted trap distribution widths. This work shows the importance of considering the specific properties of organic materials to study their properties and their trap distributions.
Published: 2021

8. Pyridazinone Derivatives Limit Osteosarcoma-Cells Growth In Vitro and In Vivo

Author: Aurélie Moniot, Françoise Rédini, Frédéric Velard, Janos Sapi, Sarah Renault, Camille Bour, Sandra Audonnet, Ingrid Allart-Simon, Julien Braux, Fabien Lamret, Christine Guillaume, Sophie C. Gangloff, Marie Laronze-Cochard, Stéphane Gérard, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Plateau Technique de Cytométrie en Flux (URCACyt), Université de Reims Champagne-Ardenne (URCA), Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes - INSERM U1238] (Phy-Os), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Colin, Marius, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université Bretagne Loire (UBL)
Subjects: Cancer Research, [SDV.CAN]Life Sciences [q-bio]/Cancer, migration, Article, 03 medical and health sciences, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Annexin, In vivo, osteosarcoma, Medicine, Cytotoxic T cell, Cytotoxicity, RC254-282, 030304 developmental biology, 0303 health sciences, business.industry, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Cancer, pyridazinone, cytotoxicity, tumor growth, medicine.disease, In vitro, 3. Good health, Oncology, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Osteosarcoma, business
Abstract: Simple Summary There is a dire need for novel therapeutic interventions to treat osteosarcoma. Pyridazinone derivatives have proven some efficacy in several cancer models, but their effect on osteosarcoma is yet to be evaluated. Our goal was to synthesize and evaluate, both in vitro and in vivo, some pyridazinone derivatives to provide a proof of concept of their potential as anti-osteosarcoma molecules. We demonstrated that our newly synthesized pyridazinone scaffold-based molecules might be hit-candidates to develop new therapeutic avenues for multi-therapy purposes. Abstract Osteosarcoma is a rare primary bone cancer that mostly affects children and young adults. Current therapeutic approaches consist of combining surgery and chemotherapy but remain unfortunately insufficient to avoid relapse and metastases. Progress in terms of patient survival has remained the same for 30 years. In this study, novel pyridazinone derivatives have been evaluated as potential anti-osteosarcoma therapeutics because of their anti-type 4 phosphodiesterase activity, which modulates the survival of several other cancer cells. By using five—four human and one murine osteosarcoma—cell lines, we demonstrated differential cytotoxic effects of four pyridazinone scaffold-based compounds (mitochondrial activity and DNA quantification). Proapoptotic (annexin V positive cells and caspase-3 activity), anti-proliferative (EdU integration) and anti-migratory effects (scratch test assay) were also observed. Owing to their cytotoxic activity in in vitro conditions and their ability to limit tumor growth in a murine orthotopic osteosarcoma model, our data suggest that these pyridazinone derivatives might be hit-candidates to develop new therapeutic strategies against osteosarcoma.
Published: 2021

9. Covalent Grafting of Ruthenium Complexes on Iron Oxide Nanoparticles: Hybrid Materials for Photocatalytic Water Oxidation

Author: Pierre Lecante, Vincent Collière, Marc Respaud, Phong D. Tran, Quyen Nguyen, Van Nguyen, Jérôme Esvan, Karine Philippot, Wantana Klysubun, Catherine Amiens, Gilles Lemercier, Elodie Rousset, Laboratoire de chimie de coordination (LCC), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), University of Science and Technology of Hanoi (USTH), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre d'élaboration de matériaux et d'études structurales (CEMES), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Synchrotron Light Research Institute (SLRI), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Laboratoire de physique et chimie des nano-objets (LPCNO), Université de Toulouse (UT)-Fédération de recherche « Matière et interactions » (FeRMI), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), CNRS, Université de Toulouse Paul-Sabatier, Vietnam Academy of Science and Technology (grant D̵L0000.03/19-21), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University of sciences and technologies of hanoi (USTH), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE), Synchrotron Light Research Institute (THAILAND), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Université des Sciences et des Technologies de Hanoi - USTH (VIETNAM), Université de Reims - Champagne-Ardenne (FRANCE), Institut de Chimie Moléculaire de Reims - ICMR (Reims, France), and Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France)
Subjects: Materials science, Matériaux, Nanoparticle, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, Photochemistry, 01 natural sciences, 7. Clean energy, Catalysis, [SPI.MAT]Engineering Sciences [physics]/Materials, Iron oxide nanoparticles, General Materials Science, Pendant group, Water oxidation catalysis, Ruthenium-based photosensitizer, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Ruthenium, chemistry, Covalent bond, Photocatalysis, Hybrid photoanode, Reversible hydrogen electrode, 0210 nano-technology, Hybrid material, Covalent grafting
Abstract: International audience; The present environmental crisis prompts the search for renewable energy sources such as solar-driven production of hydrogen from water. Herein, we report an efficient hybrid photocatalyst for water oxidation, consisting of a ruthenium polypyridyl complex covalently grafted on core/shell Fe@FeOx nanoparticles via a phosphonic acid group. The photoelectrochemical measurements were performed under 1 sun illumination in 1 M KOH. The photocurrent density of this hybrid photoanode reached 20 μA/cm2 (applied potential of +1.0 V vs reversible hydrogen electrode), corresponding to a turnover frequency of 0.02 s–1. This performance represents a 9-fold enhancement of that achieved with a mixture of Fe@FeOx nanoparticles and a linker-free ruthenium polypyridyl photosensitizer. This increase in performance could be attributed to a more efficient electron transfer between the ruthenium photosensitizer and the Fe@FeOx catalyst as a consequence of the covalent link between these two species through the phosphonate pendant group.
Published: 2021

10. Analysis and multi-response optimization of two dew point cooler configurations using the desirability function approach

Author: A.F. Boudjabi, C. Maalouf, T. Moussa, D. Abada, D. Rouag, M. Lachi, G. Polidori, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Groupe de Recherche en Sciences Pour l'Ingénieur - EA 4694 (GRESPI), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Matériaux et Ingénierie Mécanique (MATIM), Université de Reims Champagne-Ardenne (URCA), and PHC TASSILI 2018
Subjects: Optimization, SPARK, [SPI]Engineering Sciences [physics], Combined dew point coolerModelingDesirability approachOptimizationGenOptSPARK, Modeling, Combined dew point cooler, Electrical engineering. Electronics. Nuclear engineering, GenOpt, Desirability approach, TK1-9971
Abstract: International audience; Due to climate change and hot summers in most of Algeria’s regions, air conditioning has become a thorny issue, resulting in excessive energy consumption. This research focuses on the implementation of dew point evaporative coolers as an alternative to conventional air conditioners. The desirability function approach is used to predict the optimal operating parameters and compare the thermal performance and water consumption of two dew point cooler configurations (counter flow-regenerative cooler and combined parallel–regenerative cooler) under different climatic conditions in Algeria. A numerical model is developed and validated using literature data for regenerative and combined dew point evaporative systems. Parametric analysis and multi- to single-objective optimization simulations were performed with the GenOpt simulation software, identifying the most influential parameters and predicting the optimal conditions for both configurations. The findings suggest that the optimal channel spacing, working air ratio, and primary air velocity are identical for both technologies.However, the cooling capacity and the coefficient of performance of the combined parallel–regenerative cooler are higher in drier climates. When both technologies are compared at nearly equal dew point efficiency, the combined configuration improves cooling capacity by 5%, while increasing water consumption by about 40%. This study provides a useful method for assessing cooling capacity and adapting suitable dew point technology to various climates and cities.
Published: 2021

11. Antimicrobial Photodynamic Therapy: Latest Developments with a Focus on Combinatory Strategies

Author: Tony Le Gall, Ulrich Jonas, Ali Balasini, Alizé Hascoët, Franck Thétiot, Tristan Montier, Gilles Lemercier, Holger Schönherr, Mareike Müller, Max Müller, Raphaëlle Youf, INSERM Unit 1018, Universität Siegen [Siegen], Chimie, Electrochimie Moléculaires et Chimie Analytique (CEMCA), Université de Brest (UBO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Le Gall, Tony
Subjects: Computer science, medicine.medical_treatment, Pharmaceutical Science, Photodynamic therapy, Context (language use), 02 engineering and technology, Review, 010402 general chemistry, 01 natural sciences, antimicrobials, Pharmacy and materia medica, multidrug resistance, medicine, combinatory strategies, Management science, ROS, photosensitizers, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, 021001 nanoscience & nanotechnology, Antimicrobial, 0104 chemical sciences, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, RS1-441, photodynamic therapy, nanoparticles, 0210 nano-technology
Abstract: International audience; Antimicrobial photodynamic therapy (aPDT) has become a fundamental tool in modern therapeutics, notably due to the expanding versatility of photosensitizers (PSs) and the numerous possibilities to combine aPDT with other antimicrobial treatments to combat localized infections. After revisiting the basic principles of aPDT, this review first highlights the current state of the art of curative or preventive aPDT applications with relevant clinical trials. In addition, the most recent developments in photochemistry and photophysics as well as advanced carrier systems in the context of aPDT are provided, with a focus on the latest generations of efficient and versatile PSs and the progress towards hybrid-multicomponent systems. In particular, deeper insight into combinatory aPDT approaches is afforded, involving non-radiative or other light-based modalities. Selected aPDT perspectives are outlined, pointing out new strategies to target and treat microorganisms. Finally, the review works out the evolution of the conceptually simple PDT methodology towards a much more sophisticated, integrated, and innovative technology as an important element of potent antimicrobial strategies.
Published: 2021

12. Galleria mellonella as a Good Model to Study Acinetobacter baumannii Pathogenesis

Author: Tao, Ye, Duma, Luminita, Rossez, Yannick, Université de Lille, CNRS, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, GEC UTC (GEC UTC), Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Génie Enzymatique et Cellulaire. Reconnaissance Moléculaire et Catalyse - UMR CNRS 7025 (GEC UPJV), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Compiègne (UTC), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Acinetobacter baumannii, Y Acinetobacter baumannii, animal structures, Galleria mellonella, host–pathogen interactions, virulence factors, therapy strategies, [SDV]Life Sciences [q-bio], fungi, Review, biochemical phenomena, metabolism, and nutrition, Rossez, L, host-pathogen interactions, Medicine, Y, Duma, Tao, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: The invertebrate model, Galleria mellonella, has been widely used to study host–pathogen interactions due to its cheapness, ease of handling, and similar mammalian innate immune system. G. mellonella larvae have been proven to be useful and a reliable model for analyzing pathogenesis mechanisms of multidrug resistant Acinetobacter baumannii, an opportunistic pathogen difficult to kill. This review describes the detailed experimental design of G. mellonella/A. baumannii models, and provides a comprehensive comparison of various virulence factors and therapy strategies using the G. mellonella host. These investigations highlight the importance of this host–pathogen model for in vivo pathogen virulence studies. On the long term, further development of the G. mellonella/A. baumannii model will offer promising insights for clinical treatments of A. baumannii infection.
Published: 2021

13. Enhanced Resonant Raman scattering of GaN functional layers using Al thin films -a versatile tool for multilayer structure analysis

Author: Alina Muravitskaya, Anna Rumyantseva, Atse Julien Eric Ndohi, Camille Sonneville, Dominique Planson, Pierre Michel Adam, Sylvain Potiron, Mihai Lazar, Lumière, nanomatériaux et nanotechnologies (L2n), Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Ampère, Département Energie Electrique (EE), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-18-EURE-0013,NANO-PHOT,Graduate School in Nano-optics and Nanophotonics(2018), Université de Technologie de Troyes (UTT), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Planson, Dominique
Subjects: SiC, Raman scattering, thin layers, SERS, Mechanical Engineering, [SPI.NRJ]Engineering Sciences [physics]/Electric power, power device, Condensed Matter Physics, GaN, plasmonic nanoparticles, [SPI]Engineering Sciences [physics], Mechanics of Materials, nanostructures, General Materials Science, ComputingMilieux_MISCELLANEOUS, [SPI.NRJ] Engineering Sciences [physics]/Electric power
Abstract: International audience; In order to selectively analyze active thin layers close to surface in power devices structures, Raman scattering is necessary with UV excitation. However, the Raman spectra of GaN are usually affected by the direct bandgap photoluminescence of the material, which interferes with the Raman measurements and decreases the quality and resolution of the Raman spectra. In this work, we demonstrate experimentally that nanostructured aluminum films deposed on GaN epitaxial layers decrease the influence of the photoluminescence on the resonant Raman spectra and increase its overall spatial resolution under UV illumination.
Published: 2021

14. Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy against Pseudomonas aeruginosa

Author: Marius Colin, Emilie Charpentier, Ludovic Doudet, Sophie C. Gangloff, Fany Reffuveille, Ingrid Allart-Simon, Stéphane Gérard, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Colin, Marius
Subjects: Microbiology (medical), medicine.drug_class, [SDV]Life Sciences [q-bio], Antibiotics, synergy, RM1-950, medicine.disease_cause, Biochemistry, Microbiology, biofilm, 03 medical and health sciences, chemistry.chemical_compound, Antibiotic resistance, [CHIM] Chemical Sciences, medicine, [CHIM]Chemical Sciences, Pharmacology (medical), Crystal violet, General Pharmacology, Toxicology and Pharmaceutics, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Pseudomonas aeruginosa, Biofilm, indoloquinoline, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Antimicrobial, 3. Good health, Ciprofloxacin, [SDV] Life Sciences [q-bio], Infectious Diseases, chemistry, Therapeutics. Pharmacology, Bacteria, medicine.drug
Abstract: International audience; Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-b]quinoline derivatives by a domino radical process. We tested the effect of two different “indoloquinoline” molecules (Indol-1 and Indol-2) without antimicrobial activity, in addition to ciprofloxacin, on biofilm formation thanks to crystal violet staining and enumeration of adhered bacteria. This association of ciprofloxacin and Indol-1 or Indol-2 attenuated the formation of biofilm up to almost 80% compared to ciprofloxacin alone, or even prevented the presence of adhered bacteria. In conclusion, these data prove that the association of non-antimicrobial molecules with an antibiotic can be a solution to fight against biofilm and antibiotic resistance emergence.
Published: 2021

15. Antidepressants Modify Cryptic Behavior in Juvenile Cuttlefish at Environmentally Realistic Concentrations

Author: Cécile Bellanger, Thomas Knigge, Apolline Chabenat, Université de Caen Normandie (UNICAEN), Normandie Université (NU), Ethologie animale et humaine (EthoS), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Normandy Region, Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Cuttlefish, Camouflage, Sepia, Health, Toxicology and Mutagenesis, Zoology, Venlafaxine, 010501 environmental sciences, Development, Sepia officinalis, 01 natural sciences, Cephalopod, 03 medical and health sciences, Fluoxetine, medicine, Animals, Environmental Chemistry, Juvenile, [CHIM]Chemical Sciences, 14. Life underwater, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Behavior, Animal, biology, Body color changes, Decapodiformes, Venlafaxine Hydrochloride, biology.organism_classification, Antidepressive Agents, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Officinalis, [SDE]Environmental Sciences, medicine.drug
Abstract: Contamination of the marine environment by antidepressants may affect neurophysiological processes in nontarget organisms, such as the common cuttlefish, Sepia officinalis. The present study tested whether environmentally realistic concentrations of antidepressants, that is, fluoxetine alone (5 ng L-1 ) or cumulated with venlafaxine (2.5 or 5 ng L-1 ), affect camouflage in newly hatched cuttlefish. The results show that antidepressants improved uniform body patterns, whereas disruptive body patterns were not affected. Environ Toxicol Chem 2021;40:2571-2577. © 2021 SETAC.
Published: 2021

16. In situ use of bivalves and passive samplers to reveal water contamination by microcystins along a freshwater-marine continuum in France

Author: Myriam Bormans, Zouher Amzil, Emilie Lance, Véronique Savar, Alexandra Lepoutre, Elise Robert, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), This work was funded by the French National Research Program for Environmental and Occupational Health of the French Agency for Food, Environmental and Occupational Health & Safety (ANSES 2015/1/191, Bieautox program)., Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, In situ, Cyanobacteria, biosurvey, Environmental Engineering, Microcystins, Cyanotoxins, [SDV]Life Sciences [q-bio], Fresh Water, 01 natural sciences, 03 medical and health sciences, cyanotoxins, Phytoplankton, Animals, Humans, 14. Life underwater, Waste Management and Disposal, Ecosystem, 030304 developmental biology, Water Science and Technology, Civil and Structural Engineering, molluscs, 0303 health sciences, geography, geography.geographical_feature_category, biology, 010604 marine biology & hydrobiology, Ecological Modeling, Aquatic ecosystem, Solid phase adsorption toxin tracking (SPATT), fungi, Water, Estuary, Molluscs, Mussel, Contamination, biology.organism_classification, Pollution, 6. Clean water, Mytilus, Bivalvia, solid phase adsorption toxin tracking (SPATT), Biosurvey, Environmental chemistry, Environmental science
Abstract: International audience; Cyanobacteria are a potential threat to aquatic ecosystems and human health because of their ability to produce cyanotoxins, such as microcystins (MCs). MCs are regularly monitored in fresh waters, but rarely in estuarine and marine waters despite the possibility of their downstream export. Over a period of two years, we monthly analyzed intracellular (in phytoplankton) and extracellular (dissolved in water) MCs at five stations along a river continuum from a freshwater reservoir with ongoing cyanobacterial blooms to the coast of Brittany, France. MCs were quantified using two integrative samplers placed at each site: solid phase adsorption toxin tracking (SPATT) samplers for collecting extracellular MCs and caged mussels (Anodonta anatina and Mytilus edulis) filter-feeding on MC-producing cyanobacteria. The MC transfer was demonstrated each year during five months at estuarine sites and sporadically at the marine outlet. SPATT samplers integrated extracellular MCs, notably at low environmental concentrations (0.2 µg/L) and with the same variant profile as in water. The mussel A. anatina highlighted the presence of MCs including at intracellular concentrations below 1 µg/L. M. edulis more efficiently revealed the MC transfer at estuarine sites than water samplings. Bivalves showed the same MC variant profile as phytoplankton samples, but with differential accumulation capacities between the variants and the two species. Using SPATT or bivalves can give a more accurate assessment of the contamination level of a freshwater-marine continuum, in which the MC transfer can be episodic. MC content in M. edulis represents a potent threat to human health if considering updated French guideline values, and particularly the total (free and protein-bound) MC content, highlighting the necessity to include cyanotoxins in the monitoring of seafood originating from estuarine areas.
Published: 2021

17. Gradient of decomposition in sugarcane mulches of various thicknesses

Author: Guilherme Dietrich, Adriane Luiza Schu, Douglas Adams Weiler, Sandro José Giacomini, Patrick Leal Pinheiro, Mathias Roberto Leite Rambo, Sylvie Recous, Soils Department, Universidade Federal de Santa Maria (UFSM), Fractionnement des AgroRessources et Environnement - UMR-A 614 (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Brazil government through the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil government through the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Institut National de la Recherche Agronomique (INRA) - Environment & Agronomy Division, Program CNPq-Ciencia sem Fronteiras 208415/2017-3, Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), and Universidade Federal de Santa Maria = Federal University of Santa Maria [Santa Maria, RS, Brazil] (UFSM)
Subjects: Crop residue, Materials science, [SDV]Life Sciences [q-bio], décomposition, Soil Science, chemistry.chemical_element, engineering.material, sugarcane, paillis, pluviométrie, Dry matter, Water content, Chemical composition, Earth-Surface Processes, 2. Zero hunger, mulch, Brasil, 04 agricultural and veterinary sciences, dynamique de l'eau, 15. Life on land, Straw, Nitrogen, chemistry, Agronomy, crop residue, 040103 agronomy & agriculture, engineering, rainfall rate recording, 0401 agriculture, forestry, and fisheries, Fertilizer, straw mulches, Agronomy and Crop Science, Mulch, canne à sucre, évaporation
Abstract: Crop residues left on the soil surface as mulch influence many services provided in agrosystems, particularly soil protection, water dynamics and nutrient fluxes. For a given crop, the residue mass influences the mulch thickness, but the effect of thickness on decomposition is not well understood, and decomposition gradients within mulches in the field have not been described. This study aimed to quantify the decomposition of sugarcane straw mulches by varying their mass and thickness and determine the decomposition gradient within the mulches. The experiment was conducted on a first-ratoon sugarcane crop for one year, with 4, 8 and 12 Mg straw dry matter ha(-1). Mulches were displayed in 0.16-m(2) litter boxes and formed by straw layers of equivalent mass, i.e., 4 Mg ha(-1), stacked either as a single layer (Low/Top layer), 2 layers (Low and Top layers) or 3 layers (Low, Middle and Top layers) to reconstitute the 3 mulch quantities. We quantified the carbon (C) and nitrogen (N) contents, the chemical composition and the water content of the remaining mulch particles in each layer 10 times within the 360 days. The mulch degradation rates were proportional to the initial amount of straw, the mulch losses representing 75% of its initial C and 46% of its initial N after one year, regardless of the initial mass. Fertilizer-N addition did not change the k decomposition rate, with k = 0.0044 d(-1). However the decomposition rate differed according to the layer position in the mulch, e.g., in the 12 Mg ha(-1) treatment k = 0.0064 d(-1) 0.0046 d(-1) and 0.0033 d(-1) for the low, middle and top layers, respectively, indicating that the low layers in contact with the soil decomposed faster than the top layers in contact with the atmosphere, while the medium layers demonstrated intermediate behaviour. The top layers evolved similarly, regardless of mulch thickness, while the lower layers in contact with the soil decomposed more quickly as the overlying straw thickness increased. Regardless of the thickness and position of a given layer within mulch, the one-year layer C loss was linearly correlated with the average water content of that layer over the year (y = 52.79 - 0694x, R-2 = 0.991, P < 0.05). Here, we demonstrate that the dynamics of mulch decomposition is primarily driven by the dynamics of water, which is itself driven by mulch characteristics, rain regime and evaporation.
Published: 2019

18. Screening of endophytic bacteria isolated from domesticated and wild growing grapevines as potential biological control agents against crown gall disease

Author: Ali Akbar Mozafari, Behrouz Harighi, Essaid Ait Barka, Susan Asghari, Qassim Esmaeel, Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, biology, [SDV]Life Sciences [q-bio], Pseudomonas, Pantoea, Enterobacter, Agrobacterium tumefaciens, Erwinia, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Serratia, 3. Good health, 010602 entomology, Animal ecology, Insect Science, Botany, Gall, Agronomy and Crop Science
Abstract: International audience; Crown gall caused by Agrobacterium tumefaciens and Allorhizobium vitis is an important disease of grapevine worldwide. Both pathogens were isolated from infected grapevines cultivated in Iran and identified by 16S rRNA sequence analysis. A total of 314 endophytic bacteria were isolated from the root, leaves, shoots and fruits of domestic and wild growing grapevines collected from various locations of Kurdistan and West Azerbaijan Provinces. Selected strains further identified by nucleotide sequencing of the 16S rRNA gene belonged to known genera including Pseudomonas, Erwinia, Pantoea, Serratia, Enterobacter and Micrococcus. These strains were screened for their antagonistic effects on both pathogens in vitro as well as their ability to reduce gall formation in planta. In total, 17 strains were able to prevent the growth of both or one of the pathogens under in vitro condition. Among them, Pseudomonas sp. Sn48, Pseudomonas sp. Ba35 and Pantoea sp. Sa14 strains significantly reduced the size of galls in grapevine plantlets. To the best of our knowledge, this study is the first report on the diversity of endophytic bacteria of grapevines with biological control potential against crown gall disease in Iran.
Published: 2019

19. Molecular and Material Engineering of Photocathodes Derivatized with Polyoxometalate-Supported {Mo3S4} HER Catalysts

Author: Bruno Fabre, Nathalie Leclerc-Laronze, Antoine Vacher, Cristelle Mériadec, Clément Falaise, Soraya Ababou-Girard, Francis Gouttefangeas, Jeoffrey Tourneur, Loïc Joanny, Emmanuel Cadot, Mohamed Haouas, Gabriel Loget, Emmanuel Guillon, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Synthèse Caractérisation Analyse de la Matière (ScanMAT), Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Microscopie Electronique à Balayage et microAnalyse (C.M.E.B.A.), Université de Rennes (UR), Institut Lavoisier de Versailles (ILV), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), ANR (Agence Nationale de la Recherche) [ANR-15-CE06-0002-01], University of Rennes 1, University of Versailles Saint Quentin, LabEx CHARMMMAT of University Paris-Saclay [ANR-11-LABX-0039], CNRS, ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [PHYS]Physics [physics], Thesaurus (information retrieval), Chemistry, Nanotechnology, General Chemistry, 010402 general chemistry, 01 natural sciences, Biochemistry, Catalysis, 0104 chemical sciences, Molecular engineering, Search engine, Colloid and Surface Chemistry, Polyoxometalate, [CHIM]Chemical Sciences
Abstract: International audience; Molecular engineering of efficient HER catalysts is an attractive approach for controlling the spatial environment of specific building units selected for their intrinsic functionality required within the multistep HER process. As the {Mo 3 S 4 } core derived as various coordination complexes has been identified as one as the most promising MoS x-based HER electrocatalysts, we demonstrate that the covalent association between the {Mo 3 S 4 } core and the redox-active macrocyclic {P 8 W 48 } polyoxometalate (POM) produces a striking synergistic effect featured by high HER performance. Various experiments carried out in homogeneous conditions showed that this synergistic effect arises from the direct connection between the {Mo 3 S 4 } cluster and the toroidal {P 8 W 48 } units featured by a stoichiometry which can be tuned from two to four {Mo 4 S 4 } cores per {P 8 W 48 } unit. In addition, we report that this effect is preserved within heterogeneous photoelectrochemical devices where the {Mo 3 S 4 }-{P 8 W 48 } (thio-POM) assembly was used as co-catalyst (cocat) onto a microstructured p-type silicon. Using drop-casting procedure to immobilize cocat onto the silicon interface led to high initial HER performance under simulated sunlight achieving a photocurrent density of 10 mA.cm-2 at +0.13 V vs RHE. Furthermore, electrostatic incorporation of the thio-POM anion cocat into a poly(3,4-ethylenedioxythiophene) (PEDOT) film is demonstrated to be efficient and straightforward to durably retain the cocat at the interface of a micropyramidal silicon (SimPy) photocathode. The thio-POM/PEDOT-modified photocathode is able to produce H 2 under 1 Sun illumination at a rate of ca. 100 µmol cm-2 h-1 at 0 V vs RHE, highlighting the excellent performance of this photoelectrochemical system.
Published: 2019

20. The role of solvation models on the computed absorption and emission spectra: the case of fireflies oxyluciferin

Author: Madjid Zemmouche, Isabelle Navizet, Miguel-Armando Ponce-Vargas, Cristina García-Iriepa, Laboratoire de Modélisation et Simulation Multi Echelle (MSME), Université Paris-Est Marne-la-Vallée (UPEM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Biomolécules : Synthèse et Mécanismes d'Action (ICMRBSMA), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Paris-Est Marne-la-Vallée (UPEM), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Models, Molecular, Indoles, Light, Implicit solvation, General Physics and Astronomy, 02 engineering and technology, Molecular systems, 010402 general chemistry, 01 natural sciences, Molecular physics, Spectral line, Structure-Activity Relationship, Animals, [CHIM]Chemical Sciences, Molecule, Emission spectrum, Physics::Chemical Physics, Physical and Theoretical Chemistry, ComputingMilieux_MISCELLANEOUS, Physics, Molecular Structure, Fireflies, Solvation, Water, 021001 nanoscience & nanotechnology, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Spectrophotometry, Pyrazines, Luminescent Measurements, Solvents, Light emission, 0210 nano-technology, Hydrophobic and Hydrophilic Interactions, Vibrational spectra
Abstract: International audience; Surrounding effects are crucial to successfully simulate the absorption and emission spectra of molecular systems. In this work we test different solvation models to compute transition energies and to simulate the spectra of oxyluciferin responsible for the light emission in fireflies and its derivatives. We demonstrate that, within the PCM model, the IBSF formalism is suitable for computing the transition energies of the oxyluciferin chemical forms characterized by a charge transfer character. On the other hand, the LR approach could be used for the chemical forms where an almost negligible charge transfer takes place. Moreover, we demonstrate that explicit solvation models, applied by QM/MM calculations, are needed to accurately reproduce the experimental shape of the spectra. Finally, the vibrationally resolved spectra using a solvation model (implicit or microsolvation) is computed. Some noticeable differences arise when considering the implicit solvation with respect to gas phase vibrational spectra, while small changes were found when explicit water molecules within a microsolvated model are considered.
Published: 2019

21. Accelerating Metabolite Identification in Natural Product Research : Toward an Ideal Combination of Liquid Chromatography-High-Resolution Tandem Mass Spectrometry and NMR Profiling, in Silico Databases, and Chemometrics

Author: Jean-Hugues Renault, Jean-Marc Nuzillard, Jean-Luc Wolfender, Justin J. J. van der Hooft, Samuel Bertrand, Pharmaceutical Technology, École de Pharmacie Genève - Lausanne (EPGL), University of Geneva [Switzerland]-Université de Lausanne (UNIL), Chimie des Substances Naturelles (ICMRCSN), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), Mer, molécules et santé EA 2160 (MMS), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN)-Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Université de Nantes (UN)
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, Bioinformatics, In silico, Metabolite, High resolution, Tandem mass spectrometry, 01 natural sciences, Analytical Chemistry, Database, Chemometrics, 03 medical and health sciences, chemistry.chemical_compound, Metabolite profiling, Tandem Mass Spectrometry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Bioinformatica, Metabolome, Metabolomics, Life Science, Chromatography, High Pressure Liquid, Metabolite annotation, Biological Products, Natural products, Chromatography, Chemistry, 010401 analytical chemistry, Dereplication, NMR, LC-MS, 0104 chemical sciences, 030104 developmental biology, in silico, Natural Product Research, Databases, Chemical, [CHIM.CHEM]Chemical Sciences/Cheminformatics
Abstract: International audience; The rapid innovations in metabolite profiling, bioassays and chemometrics have led to a paradigm shift in natural product (NP) research. Indeed, having partial or full structure information about possibly “all” specialized metabolites and an estimation of their levels in plants or microorganisms provides a way to perform pharmacognostic or chemical ecology investigations from a new and holistic perspective. The increasing amount of accurate metabolome data that can be acquired on massive sample sets, notably through data-dependent LC-HRMS/MS and NMR profiling, allows the mapping of natural extracts at an unprecedented level of precision.Most progress made recently in accelerating metabolite identification has been pushed by the need for metabolomics to have tools that provide a confident annotation of the biomarkers highlighted as the results of data mining through multivariate analysis, often on important datasets of complexsamples. Historically, NP chemists have been involved in the unambiguous full de novo identification of unknown compoundsfrom complex natural biological matrices. This process is classically performed by the tedious isolation of pure bioactive NPs through comprehensive bioactivity-guided isolation workflows involving orthogonal chromatographic steps at the preparative level. Increasingly advanced metabolomics metabolite profiling methods are of strategic importance in dereplication workflows in NP research as well as for the full metabolome composition assignment of relevant organisms from both drug discovery and chemical ecology perspectives.In this review, we describe the latest developments in metabolite profiling by both LC-MS and NMRbased methods and related databases from a natural product chemist perspective. We assess the current possibilities and limits of such methods and the workflows for manual and automated NPannotations by equally treating the MS and NMR approaches that are both key for the “as confident as possible” NP annotation in crude natural extracts. We also propose future lines of development in the field that are important for NP research but are also generally needed for metabolite annotation in metabolomics because NPs represent perfect candidate compounds for identification due to their intrinsic structural complexity and chemodiversity across organisms. This review does not aim to provide a comprehensive survey of all metabolite profiling applications made in NP research to date. Typical case studies are discussed, and an update of a selection of the latest advanced original studies and numerous specialized reviews is made with links to tools and DBs regarded as useful for their current or future usage in NP research. Evaluations of what can be readily implemented and what is still required for confident NP structural elucidation are made, especially concerning access to generic structural and spectral DBs as well as the use of orthogonal detection methods for improved confidence in metabolite annotation.
Published: 2019

22. Taxonomy-focused Natural Product Databases for Carbon-13 NMR-based Dereplication

Author: Jean-Marc Nuzillard, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: analytical_chemistry, databases, natural products, Computer science, computer.software_genre, 01 natural sciences, Set (abstract data type), taxonomy, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Taxonomy (general), computer.programming_language, QD71-142, Natural product, Database, 010405 organic chemistry, General Medicine, Python (programming language), Carbon-13 NMR, dereplication, NMR, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Taxon, chemistry, Scripting language, Identification (biology), Analytical chemistry, computer
Abstract: International audience; The recent revival of the study of organic natural products as renewable sources of medicinal drugs, cosmetics, dyes, and materials motivated the creation of general purpose structural databases. Dereplication, the efficient identification of already reported compounds, relies on the grouping of structural, taxonomic and spectroscopic databases that focus on a particular taxon (species, genus, family, order, etc.). A set of freely available python scripts, CNMR_Predict, is proposed for the quick supplementation of taxon oriented search results from the naturaL prOducTs occUrrences database (LOTUS, lotus.naturalproducts.net) with predicted carbon-13 nuclear magnetic resonance data from the ACD/Labs CNMR predictor and DB software (acdlabs.com) to provide easily searchable databases. The database construction process is illustrated using Brassica rapa as a taxon example
Published: 2021

23. A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach

Author: Claude Plassard, Herve Quiquampoix, Patrick Chemardin, Josiane Abadie, Jean-Bernard Behr, Christophe Dussouy, Eric Dubreucq, Véronique Perrier, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), UNLOCKP project, ANR-11-BSV7-0015,UNLOCKP,Mobilisation des réserves de Phytate du sol : quelles sont les clefs nécessaires pour accéder au phosphore de ce trésor ?(2011), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro
Subjects: Models, Molecular, Phytic Acid, Biocompatibility, Phosphate, 01 natural sciences, Biochemistry, Substrate Specificity, Catalysis, chemistry.chemical_compound, Soil, Fertilizer, Artificial substrate, Drug Discovery, PEG ratio, Inositol, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Fluorescent Dyes, 6-Phytase, Phytic acid, Inositol-phosphates, Molecular Structure, 010405 organic chemistry, Chemistry, Organic Chemistry, Substrate (chemistry), Combinatorial chemistry, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Kinetics, Phosphatidylcholines, Linker, Phytases, Fluorescent tag
Abstract: International audience; A new myo-inositol pentakisphosphate was synthesized, which featured a dansyl group at position C-5. The fluorescent tag was removed from the inositol by a 6-atom spacer to prevent detrimental steric interactions in the catalytic site of phytases. The PEG linker was used in order to enhance hydrophilicity and biocompatibility of the new artificial substrate. Computational studies showed a favorable positioning in the catalytic site of phytases. Enzymatic assays demonstrated that the tethered myo-inositol was processed by two recombinant phytases Phy-A and Phy-C, classified respectively as acid and alkaline phytases, with similar rates of phosphate release compared to their natural substrate.
Published: 2021

24. The Utility of Dreissena polymorpha for Assessing the Viral Contamination of Rivers by Measuring the Accumulation of F-Specific RNA Bacteriophages

Author: Isabelle Bonnard, Christophe Gantzer, Mélissa Palos Ladeiro, Alain Geffard, Nicolas Boudaud, Sandrine Capizzi-Banas, Fanny Bastien, Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Actalia [Saint-Lô], Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Pollution, lcsh:Hydraulic engineering, animal structures, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Geography, Planning and Development, 010501 environmental sciences, Aquatic Science, 01 natural sciences, Biochemistry, Dreissena, 03 medical and health sciences, lcsh:Water supply for domestic and industrial purposes, bacteriophage, lcsh:TC1-978, 14. Life underwater, 0105 earth and related environmental sciences, Water Science and Technology, media_common, lcsh:TD201-500, 0303 health sciences, biology, 030306 microbiology, surface water, mollusk, Contamination, biology.organism_classification, 6. Clean water, bioaccumulation, Wastewater, 13. Climate action, Bioaccumulation, Environmental chemistry, biomonitoring, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Zebra mussel, Viral load, Surface water
Abstract: River water that receives treated wastewater can be contaminated by pathogens including enteric viruses due to fecal pollution, which may represent an important public health hazard. There is a great diversity of enteric viruses and fecal bacteriophages, especially F-specific RNA bacteriophages (FRNAPHs), are commonly proposed as indicators of viral pollution due to a variety of characteristics such as their structural similarities to the main enteric viruses, their high concentrations in raw wastewater and their environmental survival rate, which is better than other cultivable enteric viruses. However, evaluating the viral contamination of water on the basis of FRNAPH concentration levels continues to present a challenge. This is because the quality of detection is strongly dependent on the quantity of viral particles, high spatio-temporal variabilities and the physico-chemical conditions of the water during sampling. To overcome these limitations, the present study aims to evaluate whether the bivalve mollusk Dreissena polymorpha (zebra mussel) could be considered a suitable experimental model for assessing the viral contamination of rivers. In order to determine this, the capacity of D. polymorpha to accumulate FRNAPHs and assimilate them into their soft tissue was studied. This provided a proof of concept for the use of D. polymorpha to evaluate the viral contamination of surface water. Two experiments were conducted: (1) an in situ experiment to confirm that zebra mussels naturally accumulated FRNAPHs and (2) a laboratory experiment to determine the accumulation and depuration kinetics of FRNAPHs in D. polymorpha tissue. The study highlights the capacity of the mussels to accumulate infectious FRNAPHs both on a laboratory scale under controlled conditions as well as in situ at different sites that are representative of different bodies of water. An analysis of the mussels’ soft tissue showed that they were capable of reflecting the water’s contamination level very quickly (within less than 24 h). Moreover, the soft tissue retained the viral load much longer than the water due to a low depuration rate. The analysis of FRNAPH concentrations in mussels exposed in situ suggested that there were differences in contamination levels between sites. These preliminary results underline the potential utility of zebra mussels in assessing viral contamination by measuring the accumulation of FRNAPHs in their tissue. This may ultimately enable stakeholders to use zebra mussels as a means of monitoring viral pollution in surface water.
Published: 2021

25. Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots

Author: Igor Nabiev, Alyona Sukhanova, Klervi Even-Desrumeaux, Patrick Chames, Daniel Baty, Mikhail Artemyev, Vladimir Oleinikov, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Belarusian State University, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh RAS), Russian Academy of Sciences [Moscow] (RAS), European Project: 246479,EC:FP7:NMP,FP7-NMP-2009-LARGE-3,NAMDIATREAM(2010), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Nabiev, Igor, and NANOTECHNOLOGICAL TOOLKITS FOR MULTI-MODAL DISEASE DIAGNOSTICS AND TREATMENT MONITORING - NAMDIATREAM - - EC:FP7:NMP2010-07-01 - 2014-06-30 - 246479 - VALID
Subjects: medicine.drug_class, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Nanoparticle, Nanotechnology, quantum dots, 02 engineering and technology, Conjugated system, 010402 general chemistry, Monoclonal antibody, 01 natural sciences, Flow cytometry, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, General Environmental Science, Plasmonic nanoparticles, diagnostic nanoprobes, Bioconjugation, medicine.diagnostic_test, cancer diagnostics, Chemistry, flow cytometry, single-domain antibodies, imaging, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Quantum dot, immunohistochemistry, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], General Earth and Planetary Sciences, 0210 nano-technology, oriented conjugation, Conjugate
Abstract: International audience; Nanoparticle-based biodetection commonly employs monoclonal antibodies (mAbs) for targeting. Although several types of conjugates have been used for biomarker labeling, the large size of mAbs limits the number of ligands per nanoparticle, impedes their intratumoral distribution, and limits intracellular penetration. Furthermore, the conditions of mAb conjugation using conventional techniques provide nanoprobes with irregular orientation of mAbs on the nanoparticle surface and often provoke mAb unfolding. Here, we have developed a protocol to engineer ultrasmall diagnostic nanoprobes through directional conjugation of semiconductor quantum dots (QDs) with 13-kDa single-domain antibodies (sdAbs) derived from llama immunoglobulin G (IgG). sdAbs are conjugated with QDs in a highly oriented manner via an additional cysteine residue specifically integrated into the sdAb C-terminus. The resultant nanoprobes are
Published: 2021

26. Dereplication of Natural Extracts Diluted in Propylene Glycol, 1,3-Propanediol and Glycerin. Comparison of Leontopodium alpinum Cass. (Edelweiss) Extracts as a Case Study

Author: Canton, Marine, Poigny, Stéphane, Roe, Richard, Nuzillard, Jean-Marc, Renault, Jean-Hugues, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Pharmacochimie Pierre Fabre Dermo-Cosmétique, Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE-PIERRE FABRE, Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Centre de Recherche Pierre Fabre
Subjects: glycerin, dereplication, NMR, mixture analysis, lcsh:Chemistry, centrifugal partition chromatography, lcsh:QD1-999, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, propylene glycol, Leontopodium alpinum, natural extract analysis, 1,3-propanediol, ComputingMilieux_MISCELLANEOUS, [CHIM.CHEM]Chemical Sciences/Cheminformatics
Abstract: Many natural extracts used as cosmetic ingredients are available as solutions prepared in high-boiling-point solvents, called carrier solvents, such as propylene glycol (1,2-propanediol), propanediol (1,3-propanediol) and glycerin. The upstream chemical profiling of these extracts represents a major asset for the cosmetic industry, because it accelerates product development. A new workflow for the rapid characterization of the main metabolites present in natural extracts diluted in propylene glycol and 1,3-propanediol is presented here as an extension of previous works on glycerin-containing extracts. This method is an optimized version of a well-established dereplication procedure and consists of a fractionation by centrifugal partition chromatography followed by 13C nuclear magnetic resonance analysis and dedicated data processing. The concentration by evaporation under reduced pressure was considered as a pertinent preliminary step, particularly adapted to the analysis of highly diluted extracts. A dried hydro-ethanolic extract of Leontopodium alpinum Cass. was prepared at laboratory scale and used for method validation. Three solutions at 5% wt. of dry extract were prepared with propylene glycol/water (1:1), 1,3-propanediol/water (1:1) and glycerin/water (1:1) as carrier solvents. The dereplication workflow was applied to the three resulting L. alpinum extracts. Each study led to the quick identification of 26 metabolites including five flavonoids (luteolin and its derivatives), five hydroxycinnamic acids (among which are leontopodic acids), sugars and organic acids.
Published: 2021

27. Molecular engineering for optical properties of 5-substituted-1,10-phenanthroline-based Ru(II) complexes

Author: Philippe C. Gros, Latévi Max Lawson-Daku, Mireille Blanchard-Desce, Gilles Lemercier, Olivier Mongin, Juliette Moreau, Marc Beley, Sylviane Chevreux, Elodie Rousset, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Genève (UNIGE), Laboratoire Lorrain de Chimie Moléculaire (L2CM), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Genève = University of Geneva (UNIGE), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and PRC-CE07-033 IsoGate Grant, Agence Nationale de la Recherche
Subjects: 010405 organic chemistry, Ligand, Phenanthroline, Charge (physics), 010402 general chemistry, 01 natural sciences, Redox, 0104 chemical sciences, Molecular engineering, Inorganic Chemistry, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Crystallography, chemistry.chemical_compound, chemistry, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Absorption (chemistry)
Abstract: International audience; A series of homo- and heteroleptic Ru(II) complexes [Ru(phen)3−n(phen-X)n](PF6)2 (n = 0–3, X = CN, epoxy, H, NH2) were prepared and characterized. The influence of electron-withdrawing or electron-releasing substituents of the 1,10-phenanthroline ligands on the photo-physical properties was evaluated. It reveals fundamental interests in the fine tuning of redox potentials and photo-physical characteristics, depending both on the nature of the substitution of the ligand, and on the symmetry of the related homo- or heteroleptic complex. These complexes exhibit linear absorption and two-photon absorption (2PA) cross-sections over a broad range of wavelength (700–900 nm) due to absorption in the intra-ligand charge transfer (ILCT) and the metal-to-ligand charge transfer (MLCT) bands. These 2PA properties were more particularly investigated in the 700–1000 spectral range for a family of complexes bearing electro-donating ligands (phen-NH2).
Published: 2021

28. Revealing the elasticity of an individual aortic fiber during ageing at nanoscale by in situ atomic force microscopy †

Author: Laurent Martiny, Sébastien Blaise, Alexandre Berquand, Laëtitia Gorisse, Philippe Gillery, Laurent Duca, Béatrice Romier-Crouzet, Pascal Maurice, Christine Pietrement, Fatouma Touré, Hervé Sartelet, Michael Molinari, Stéphane Jaisson, Aubéri Henry, Amar Bennasroune, Amandine Wahart, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Contrôle de la Réponse Immune B et des Lymphoproliférations (CRIBL), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and MAURICE, Pascal
Subjects: Fluorescence-lifetime imaging microscopy, Aging, Materials science, 030204 cardiovascular system & hematology, Pulse Wave Analysis, Microscopy, Atomic Force, Extracellular matrix, 03 medical and health sciences, Mice, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, medicine.artery, medicine, Animals, General Materials Science, Elasticity (economics), [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Pulse wave velocity, Aorta, 030304 developmental biology, 0303 health sciences, biology, [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Stiffness, Elasticity, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Ageing, biology.protein, medicine.symptom, Elastin, Biomedical engineering
Abstract: International audience; Arterial stiffness is a complex process affecting the aortic tree that significantly contributes to cardiovascular diseases (systolic hypertension, coronary artery disease, heart failure or stroke). This process involves a large extracellular matrix remodeling mainly associated with elastin content decrease and collagen content increase. Additionally, various chemical modifications that accumulate with ageing have been shown to affect long-lived assemblies, such as elastic fibers, that could affect their elasticity. To precisely characterize the fiber changes and the evolution of its elasticity with ageing, high resolution and multimodal techniques are needed for precise insight into the behavior of a single fiber and its surrounding medium. In this study, the latest developments in atomic force microscopy and the related nanomechanical modes are used to investigate the evolution and in a near-physiological environment, the morphology and elasticity of aorta cross sections obtained from mice of different ages with an unprecedented resolution. In correlation with more classical approaches such as pulse wave velocity and fluorescence imaging, we demonstrate that the relative Young's moduli of elastic fibers, as well as those of the surrounding areas, significantly increase with ageing. This nanoscale characterization presents a new view on the stiffness process, showing that, besides the elastin and collagen content changes, elasticity is impaired at the molecular level, allowing a deeper understanding of the ageing process. Such nanomechanical AFM measurements of mouse tissue could easily be applied to studies of diseases in which elastic fibers suffer pathologies such as atherosclerosis and diabetes, where the precise quantification of fiber elasticity could better follow the fiber remodeling and predict plaque rupture. † Electronic supplementary information (ESI) available. See
Published: 2021

29. Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

Author: Mochalov, Konstantin, Vaskan, Ivan, Sarychev, Andrey, Ivanov, Alexandre, Bykov, Igor, Bakholdin, Nikolai, Vasina, Daria, Gushchin, Vladimir, Tkachuk, Artem, Nifontova, Galina, Samokhvalov, Pavel, Sukhanova, Alyona, Nabiev, Igor, Nabiev, Igor, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh RAS), Russian Academy of Sciences [Moscow] (RAS), Institute of Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow Power Engineering Institute (MPEI), Gamaleya Research Center, The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia], Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)
Subjects: [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Abstract: Surface-enhanced Raman scattering (SERS) spectroscopy is a surface- or cavity-enhanced variant of Raman scattering spectroscopy allowing the detection of analytes with a sensitivity down to single molecules. The main prerequisite for the effectiveness of this method is the development of SERS-active surfaces or cavities capable of concentrating incident radiation into small mode volumes containing the analyte. Here, we have demonstrated that concentration of light in an ultranarrow metal–dielectric nano-cavity between a film of the receptor-binding domain (RBD) of SARS-CoV-2 S glycoprotein and silver surface, which is formed via interaction of reduced sulfhydryl groups of the RBD with silver, allows recording SERS spectra at concentrations sufficient for ultrasensitive detection of viral protein antigens at physiologically relevant (sub-picogram) levels. Moreover, an additional increase in the cavity Q-factor upon coating the dielectric RBD film with a nanometer-thick silver shell results in an additional order(s) of magnitude enhancement of the protein SERS signal, thus ensuring a sub-femtogram sensitivity of the viral antigen detection. A simplified theoretical model explaining the observed additional enhancement of the SERS signal from the protein coated with a silver film is proposed.Our study is the first to obtain characteristic Raman and SERS spectra of the RBD of S glycoprotein, the key SARS-CoV-2 viral antigen directly, without the use of low-molecular-weight Raman-reporter molecules. The possibility of direct recording of characteristic spectra of viral protein antigens at the concentration orders of magnitude lower than those required for the detection of the whole virus in biological media makes the development of a high-performance optical method for detecting and conformational analyzing the pathogen variants a realistic task.
Published: 2021

30. The Three Pillars of Natural Product Dereplication. Alkaloids from the Bulbs of Urceolina peruviana (C. Presl) J.F. Macbr. as a Preliminary Test Case

Author: Mariacaterina Lianza, Ritchy Leroy, Carine Machado Rodrigues, Nicolas Borie, Charlotte Sayagh, Simon Remy, Stefan Kuhn, Jean-Hugues Renault, Jean-Marc Nuzillard, University of Bologna, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), De Montfort University [Leicester, United Kingdom] (DMU), Lianza M., Leroy R., Machado Rodrigues C., Borie N., Sayagh C., Remy S., Kuhn S., Renault J.-H., Nuzillard J.-M., Alma Mater Studiorum University of Bologna (UNIBO), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Natural products, natural product, spectroscopy, Biological Products, databases, Molecular Structure, [CHIM.ORGA]Chemical Sciences/Organic chemistry, natural products, Databases, Pharmaceutical, Amaryllidaceae, Secondary Metabolism, dereplication, Plant Roots, Article, molecular structures, lcsh:QD241-441, taxonomy, Alkaloids, Computational Chemistry, lcsh:Organic chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, database, ComputingMilieux_MISCELLANEOUS, [CHIM.CHEM]Chemical Sciences/Cheminformatics
Abstract: open access article The role and importance of the identification of natural products are first discussed in the perspective of the study of secondary metabolites. The rapid identification of already reported compounds, or structural dereplication, is recognized as a key element in natural product chemistry. The biological taxonomy of metabolite producing organisms, the knowledge of metabolite molecular structures, and the availability of metabolite spectroscopic signatures are considered as the three pillars of structural dereplication. The role and the construction of databases is illustrated by references to the KNApSAcK, UNPD, CSEARCH, and COCONUT databases, and by the importance of calculated taxonomic and spectroscopic data as substitutes for missing or lost original ones. Two NMR-based tools, the PNMRP database that derives from UNPD, and KnapsackSearch, a database generator that provides taxonomically focused libraries of compounds, are proposed to the community of natural product chemists. The study of the alkaloids from Urceolina peruviana, a plant from the Andes used in traditional medicine for antibacterial and anticancer actions, has given the opportunity to test different approaches to dereplication, favoring the use of publicly available data sources.
Published: 2021

31. An optimized LC-HRMS untargeted metabolomics workflow for multi-matrices investigations in the three-spined stickleback

Author: Emmanuelle Lebeau-Roche, Gaëlle Daniele, Aurélie Fildier, Cyril Turies, Odile Dedourge-Geffard, Jean-Marc Porcher, Alain Geffard, Emmanuelle Vulliet, TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut National de l'Environnement Industriel et des Risques (INERIS), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS)
Subjects: Gills, Male, Ionization, Respiratory System, RPLC, Biochemistry, Mass Spectrometry, Workflow, Metabolites, Medicine and Health Sciences, Animal Anatomy, HILIC, Gasterosteus aculeatus, ComputingMilieux_MISCELLANEOUS, Chemical Reactions, Eukaryota, Brain, Smegmamorpha, Chemistry, Liver, Osteichthyes, Physical Sciences, Vertebrates, [SDE]Environmental Sciences, Amino Acid Analysis, Metabolome, Medicine, Female, Anatomy, Research Article, Fish Biology, Science, Research and Analysis Methods, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Alkanes, Metabolomics, Animals, [CHIM]Chemical Sciences, Molecular Biology Techniques, Molecular Biology, three-spined stickleback, Sticklebacks, Molecular Biology Assays and Analysis Techniques, Organisms, Chemical Compounds, LC-QToF, Biology and Life Sciences, Hydrocarbons, untargeted metabolomics, Metabolism, Fish, Aquatic Respiratory Anatomy, Zoology, Chromatography, Liquid
Abstract: Environmental metabolomics has become a growing research field to understand biological and biochemical perturbations of organisms in response to various abiotic or biotic stresses. It focuses on the comprehensive and systematic analysis of a biologic system's metabolome. This allows the recognition of biochemical pathways impacted by a stressor, and the identification of some metabolites as biomarkers of potential perturbations occurring in a body. In this work, we describe the development and optimization of a complete reliable methodology based on liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) for untargeted metabolomics studies within a fish model species, the three-spined stickleback (Gasterosteus aculeatus). We evaluated the differences and also the complementarities between four different matrices (brain, gills, liver and whole fish) to obtain metabolome information. To this end, we optimized and compared sample preparation and the analytical method, since the type and number of metabolites detected in any matrix are closely related to these latter. For the sample preparation, a solid-liquid extraction was performed on a low quantity of whole fish, liver, brain, or gills tissues using combinations of methanol/water/heptane. Based on the numbers of features observed in LC-HRMS and on the responses of analytical standards representative of different metabolites groups (amino acids, sugars…), we discuss the influence of the nature, volume, and ratio of extraction solvents, the sample weight, and the reconstitution solvent. Moreover, the analytical conditions (LC columns, pH and additive of mobile phases and ionization modes) were also optimized so as to ensure the maximum metabolome coverages. Thus, two complementary chromatographic procedures were combined in order to cover a broader range of metabolites: a reversed phase separation (RPLC) on a C18 column followed by detection with positive ionization mode (ESI+) and a hydrophilic interaction chromatography (HILIC) on a zwitterionic column followed by detection with negative ionization mode (ESI-). This work provides information on brain, gills, liver, vs the whole body contribution to the stickleback metabolome. These information would help to guide ecotoxicological and biomonitoring studies.
Published: 2021

32. Carbamylation of elastic fibers is a molecular substratum of aortic stiffness

Author: Doué, Manon, Okwieka, Anaïs, Berquand, Alexandre, Gorisse, Laëtitia, Maurice, Pascal, Velard, Frédéric, Terryn, Christine, Molinari, Michaël, Duca, Laurent, Piétrement, Christine, Gillery, Philippe, Jaisson, Stéphane, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Reims (CHU Reims), Tis study was funded by the University of Reims Champagne-Ardenne and the Reims University Hospital through the 'Projet Hospitalo Universitaire VIeillissement protéique et VAsculaire (PHU-VIVA)' program and was also supported by grants from the Committee of American Memorial Hospital (Reims, France and Boston, MA, USA) and the Centre National de la Recherche Scientifque (CNRS, France). Te region Grand Est, the FEDERprogram, and the DRRT Grand Est are acknowledged for the funding of the Nano’Mat platform., Velard, Frédéric, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Protein Carbamylation, Science, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Elastic Tissue, Article, Cardiovascular biology, Elastin, Atomic force microscopy, Mice, Vascular Stiffness, [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Medicine, Animals, Cattle, Vascular diseases, Aorta, Cyanates, Chemical modification
Abstract: International audience; Because of their long lifespan, matrix proteins of the vascular wall, such as elastin, are subjected to molecular aging characterized by non-enzymatic post-translational modifications, like carbamylation which results from the binding of cyanate (mainly derived from the dissociation of urea) to protein amino groups. While several studies have demonstrated a relationship between increased plasma concentrations of carbamylated proteins and the development of cardiovascular diseases, molecular mechanisms explaining the involvement of protein carbamylation in these pathological contexts remain to be fully elucidated. The aim of this work was to determine whether vascular elastic fibers could be carbamylated, and if so, what impact this phenomenon would have on the mechanical properties of the vascular wall. Our experiments showed that vascular elastin was carbamylated in vivo. Fiber morphology was unchanged after in vitro carbamylation, as well as its sensitivity to elastase degradation. In mice fed with cyanate-supplemented water in order to increase protein carbamylation within the aortic wall, an increased stiffness in elastic fibers was evidenced by atomic force microscopy, whereas no fragmentation of elastic fiber was observed. In addition, this increased stiffness was also associated with an increase in aortic pulse wave velocity in ApoE −/− mice. These results provide evidence for the carbamylation of elastic fibers which results in an increase in their stiffness at the molecular level. These alterations of vessel wall mechanical properties may contribute to aortic stiffness, suggesting a new role for carbamylation in cardiovascular diseases.
Published: 2021

33. Tuning the Regioselective Functionalization of Trifluoromethylated Dienes via Lanthanum‐Mediated Single C−F Bond Activation

Author: Yan Yang, Fabien Massicot, Marc Taillefer, Florian Jaroschik, Yassir Zaid, Dominique Harakat, Elodie Rousset, Laurent Maron, Sirine Sghaier, Jean-Bernard Behr, Tarun Kumar, Agathe Martinez, Xavier Le Goff, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique et chimie des nano-objets (LPCNO), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Fédération de recherche « Matière et interactions » (FeRMI), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Etude de la Matière en Mode Environnemental (L2ME), Institut de Chimie Séparative de Marcoule (ICSM - UMR 5257), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut de Recherche sur les Systèmes Atomiques et Moléculaires Complexes (IRSAMC), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: chemistry.chemical_classification, Lanthanide, Diene, 010405 organic chemistry, Chemistry, Alkene, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Organic Chemistry, Regioselectivity, chemistry.chemical_element, General Chemistry, [CHIM.CATA]Chemical Sciences/Catalysis, 010402 general chemistry, 01 natural sciences, Combinatorial chemistry, Catalysis, 0104 chemical sciences, Metal, chemistry.chemical_compound, visual_art, visual_art.visual_art_medium, Lanthanum, Stereoselectivity, Fulvene
Abstract: International audience; The development of new fluorine-containing building blocks and their efficient synthetic access is currently a challenging research field. Herein, the highly regio- and stereoselective addition of a large range of aldehydes onto trifluoromethylated benzofulvenes was achieved using a simple La/I2/DIBAL-Cl system via a selective C−F bond activation process. This versatile methodology provided homodienyl alcohols bearing a terminal CF2-alkene with potential further applications, as shown by the dehydration to the first benzofulvenes carrying a difluorovinyl group. In addition, for certain electron-poor aldehydes, unprecedented ipso substitution of the CF3 group in a diene was observed, which, according to DFT studies, is related to the presence of the large, Lewis-acidic lanthanum metal.
Published: 2021

34. Pilot continuous centrifugal liquid-liquid extraction of extra virgin olive oil biophenols and gram-scale recovery of pure oleocanthal, oleacein, MFOA, MFLA and hydroxytyrosol

Author: Lemonia Antoniadi, Apostolis Angelis, Dimitris Michailidis, Panagiotis Stathopoulos, Leandros Skaltsounis, Jean-Marc Nuzillard, Jean-Hugues Renault, Vasiliki Tsantila, Department of Pharmaceutical Technology, National and Kapodistrian University of Athens, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Department of Pharmacognosy and Chemistry of Natural Products, National and Kapodistrian University of Athens (NKUA), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Oleacein, Oleocanthal, Filtration and Separation, Fractionation, 010501 environmental sciences, Centrifugal extractor, 01 natural sciences, High-performance liquid chromatography, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, [CHIM.GENI]Chemical Sciences/Chemical engineering, Oleuropein, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Extra virgin olive oil, [SDV.IDA]Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Chromatography, Extraction (chemistry), Centrifugal partition chromatography, Continuous liquid-liquid extraction, Aglycone, chemistry, Annular centrifugal extractor, Hydroxytyrosol
Abstract: International audience; This study provides an efficient pilot-scale liquid-liquid extraction process for the recovery of total phenolic fraction (TPF) from extra virgin olive oil (EVOO) and the further purification of the major TPF constituents (oleocanthal, oleacein, monoaldehydic form of oleuropein aglycone, monoaldehydic form of ligstroside aglycone and hydroxytyrosol) using successively centrifugal partition chromatography and reversed phase high performance liquid chromatography. Special attention was paid for the selection of the appropriate raw material, which should be rich in the targeted high-value compounds. For the extraction of the selected EVOOs, a green technique based on liquid-liquid partitioning using the biphasic system n-Hept/EVOO/EtOH/H2O 3:2:3:2 v/v was carried out, first on pioneering laboratory-scale annular centrifugal extractor (ACE) and then on pilot device. 240 L from three different EVOOs (3x80 L of each EVOO) were extracted yielding 285 g of TPF in approximately 2 h. The second step of this procedure was the purification of the target compounds. A preparative centrifugal partition chromatography (CPC) process was firstly applied for the initial TPF fractionation using a step gradient elution-extrusion method with a series of 4 biphasic systems composed of n-Hept/EtOAc/EtOH/H2O in ratios 4:1:3:2, 3:2:3:2, 2:3:3:2 and 1:4:3:2 (v/v/v/v). Preparative reversed phase high performance liquid chromatography (prep-RP-HPLC) was then carried out to polish the target compounds from the enriched CPC fractions resulting in purity higher than 97%. In parallel, the above purification analysis led to the isolation of four minor TPF constituents of which three are new natural products (EDA lactone, (1R, 8E)-1-ethoxy-ligstroside aglycon and (1S, 8E)-1-ethoxy-ligstroside aglycon) and one ((9E, 11E)-13-oxotrideca-9,11-diencoic acid) is described for the first time as EVOO constituent.
Published: 2021

35. Photochemical radical cyclization reactions with imines, hydrazones, oximes and related compounds

Author: Mohammed Latrache, Norbert Hoffmann, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Reaction conditions, Reaction mechanism, 010405 organic chemistry, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Photoredox catalysis, Structural diversity, Context (language use), General Chemistry, 010402 general chemistry, Photochemistry, 01 natural sciences, Radical cyclization, 0104 chemical sciences, Catalysis, Reagent
Abstract: International audience; Photochemical reactions are a key method to generate radical intermediates. Often under these conditions no toxic reagents are necessary. During recent years, photo-redox catalytic reactions considerably push this research domain. These reaction conditions are particularly mild and safe which enables the transformation of poly-functional substrates into complex products. The synthesis of heterocyclic compounds is particularly important since they play an important role in the research of biologically active products. In this review, photochemical radical cyclization reactions of imines and related compounds such as oximes, hydrazones and chloroimines are presented. Reaction mechanisms are discussed and the structural diversity and complexity of the products are presented. Radical intermediates are mainly generated in two ways: (1) Electronic excitation is achieved by light absorption of the substrates. (2) The application of photoredox catalysis is now systematical studied for these reactions. Recently, also excitation of charge transfer complexes have been studied in this context with many perspectives.
Published: 2021

36. Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Single-Domain Antibodies

Author: Daniel Baty, Pavel Sokolov, Igor Nabiev, Patrick Chames, Alyona Sukhanova, Fernanda Ramos-Gomes, Frauke Alves, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Max Planck Institute for Experimental Medicine [Göttingen, Germany], Aix Marseille Université (AMU), The National Research Nuclear University MEPhI (Moscow Engineering Physics Institute) [Moscow, Russia], Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, and Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Single-domain antibodies, 03 medical and health sciences, 0302 clinical medicine, medicine, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 030304 developmental biology, 0303 health sciences, medicine.diagnostic_test, Quantum dots, business.industry, Ultrasound, technology, industry, and agriculture, Magnetic resonance imaging, equipment and supplies, Deep tissue imaging, Photobleaching, Fluorescence, Micrometastases, 3. Good health, Positron emission tomography, Quantum dot, 030220 oncology & carcinogenesis, Multiphoton imaging, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Disseminated tumor cells, business, Emission computed tomography, Biomedical engineering, Conjugate
Abstract: International audience; Early detection of malignant tumors, micrometastases, and disseminated tumor cells is one of the effective way of fighting cancer. Among the many existing imaging methods like computed tomography (CT), ultrasound (US), magnetic resonance imaging (MRI), positron emission tomography (PET), and single-photon emission computed tomography (SPECT), optical imaging with fluorescent probes is one of the most promising alternatives because it is fast, inexpensive, safe, sensitive, and specific. However, traditional fluorescent probes, based on organic fluorescent dyes, suffer from the low signal-to-noise ratio. Furthermore, conventional organic fluorescent dyes are unsuitable for deep tissue imaging because of the strong visible light absorption by biological tissues. The use of fluorescent semiconductor nanocrystals, or quantum dots (QDs), may overcome this limitation due to their large multiphoton cross section, which ensures efficient imaging of thick tissue sections inaccessible with conventional fluorescent probes. Moreover, the lower photobleaching and higher brightness of fluorescence signals from QDs ensures a much better discrimination of positive signals from the background. The use of fluorescent nanoprobes based on QDs conjugated to uniformly oriented high-affinity single-domain antibodies (sdAbs) may significantly increase the sensitivity and specificity due to better recognition of analytes and deeper penetration into tissues due to small size of such nanoprobes.Here, we describe a protocol for the fabrication of nanoprobes based on sdAbs and QDs, preparation of experimental xenograft mouse models for quality control, and multiphoton imaging of deep-tissue solid tumors, micrometastases, and disseminated tumor cells.
Published: 2021

37. Transmembrane Peptides as a New Strategy to Inhibit Neuraminidase-1 Activation

Author: Albrecht, Camille, Kuznetsov, Andrey, Appert-Collin, Aline, Dhaideh, Zineb, Callewaert, Maïté, Bershatsky, Yaroslav, Urban, Anatoly, Bocharov, Eduard, Bagnard, Dominique, BAUD, Stéphanie, Blaise, Sébastien, Romier-Crouzet, Béatrice, Efremov, Roman, Dauchez, Manuel, Duca, Laurent, Guéroult, Marc, Maurice, Pascal, Bennasroune, Amar, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA), Russian Academy of Sciences [Moscow] (RAS), National Research University Higher School of Economics [Moscow] (HSE), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Biopathologie de la Myéline, Neuroprotection et Stratégies Thérapeutiques (BMNST), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Strasbourg (UNISTRA), Centre de recherche du médicament Medalis - Drug Discovery Center [LabEx], Fédération de Médecine Translationnelle de Strasbourg (FMTS), Vysšaja škola èkonomiki = National Research University Higher School of Economics [Moscow] (HSE), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), MAURICE, Pascal, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Strasbourg (UNISTRA)
Subjects: Cell and Developmental Biology, elastin receptor complex, interfering peptides, interfering peptide, Sciences du Vivant [q-bio]/Biologie cellulaire, membrane protein dimerization, sialidase activity, transmembrane domain, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, neuraminidase-1, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, Original Research
Abstract: Sialidases, or neuraminidases, are involved in several human disorders such as neurodegenerative, infectious and cardiovascular diseases, and cancers. Accumulative data have shown that inhibition of neuraminidases, such as NEU1 sialidase, may be a promising pharmacological target, and selective inhibitors of NEU1 are therefore needed to better understand the biological functions of this sialidase. In the present study, we designed interfering peptides (IntPep) that target a transmembrane dimerization interface previously identified in human NEU1 that controls its membrane dimerization and sialidase activity. Two complementary strategies were used to deliver the IntPep into cells, either flanked to a TAT sequence or non-tagged for solubilization in detergent micelles. Combined with molecular dynamics simulations and heteronuclear nuclear magnetic resonance (NMR) studies in membrane-mimicking environments, our results show that these IntPep are able to interact with the dimerization interface of human NEU1, to disrupt membrane NEU1 dimerization and to strongly decrease its sialidase activity at the plasma membrane. In conclusion, we report here new selective inhibitors of human NEU1 of strong interest to elucidate the biological functions of this sialidase.
Published: 2020

38. Alteration of predatory behaviour and growth in juvenile cuttlefish by fluoxetine and venlafaxine

Author: Apolline Chabenat, Thomas Knigge, Flavie Bidel, Cécile Bellanger, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Ethologie animale et humaine (EthoS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem (HUJ), This research work was supported by a doctoral grant from Normandy Region provided by the Research Federation CNRS 3730 SCALE (SCiences Appliquées à L'Environnement), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Cuttlefish, Environmental Engineering, food intake, Health, Toxicology and Mutagenesis, 0208 environmental biotechnology, Prey detection, Population, Zoology, 02 engineering and technology, 010501 environmental sciences, Biology, Sepia officinalis, 01 natural sciences, Predation, monoamines, Fluoxetine, Environmental Chemistry, Juvenile, Animals, 14. Life underwater, Sepia, education, Hatchling, cephalopod, development, 0105 earth and related environmental sciences, education.field_of_study, antidepressant, Hatching, maturation, [SCCO.NEUR]Cognitive science/Neuroscience, Public Health, Environmental and Occupational Health, Decapodiformes, Venlafaxine Hydrochloride, General Medicine, General Chemistry, Pollution, 020801 environmental engineering, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Predatory Behavior, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Water Pollutants, Chemical
Abstract: International audience; Antidepressants in coastal waters may affect ontogeny of predatory behaviour in cuttlefish, which may, as a result, affect growth of newly-hatched cuttlefish. We investigated the effects of two of the most prescribed antidepressants, fluoxetine (FLX) and venlafaxine (VEN) in environmentally realistic concentrations on the predatory behaviour of hatchlings of Sepia officinalis. Newly-hatched cuttlefish were exposed from 1 hour (i.e., day 1) to 5 days after hatching to either FLX alone (5 ng·L-1) combined with VEN (2.5 ng·L-1 or 5 ng·L-1 each) to simulate an environmentally realistic exposure scenario. Their predatory behaviour was analysed through several parameters: prey detection, feeding motivation and success in catching the prey. All parameters improved in control animals over the first five days. The combination of FLX and VEN at 5 ng·L-1 each altered the predatory behaviour of the hatchlings by increasing the latency before attacking the prey, i.e., reducing feeding motivation, as well as by reducing the number of successful attacks. The changes in predatory behaviour tended to reduce food intake and affected growth significantly at 28 days post-hatching. Exposures to either FLX at 5 ng·L-1 or FLX and VEN in mixture at 2.5 ng·L-1 each tended to produce similar effects, even though they were not statistically significant. It is likely that the antidepressants affect maturation of the predatory behaviour and/or learning processes associated with the development of this behaviour. The slightest delay in maturation processes may have detrimental consequences for growth and population fitness.
Published: 2020

39. Metabolic, cellular and defense responses to single and co-exposure to carbamazepine and methylmercury in Dreissena polymorpha

Author: Clément Baratange, Séverine Paris-Palacios, Isabelle Bonnard, Laurence Delahaut, Dominique Grandjean, Laurence Wortham, Stéphanie Sayen, Andrea Gallorini, Jean Michel, David Renault, Florian Breider, Jean-Luc Loizeau, Claudia Cosio, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), School of Architecture, Civil and Environmental Engineering (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250 (P3CELL), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Genève = University of Geneva (UNIGE), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, and Cosio, Claudia
Subjects: [SDE] Environmental Sciences, ddc:333.7-333.9, Gills, Toxicity pathways, Health, Toxicology and Mutagenesis, Bivalve, General Medicine, Methylmercury Compounds, Toxicology, Bioaccumulation, Pollution, Cellular compensation, Dreissena, Carbamazepine, Oxidative stress, [SDE]Environmental Sciences, ddc:550, Animals, Water Pollutants, Chemical
Abstract: International audience; Carbamazepine (CBZ) and Hg are widespread and persistent micropollutants in aquatic environments. Both pollutants are known to trigger similar toxicity mechanisms, e.g. reactive oxygen species (ROS) production. Here, their effects were assessed in the zebra mussel Dreissena polymorpha, frequently used as a freshwater model in ecotoxicology and biomonitoring. Single and co-exposures to CBZ (3.9 µg•L-1) and MeHg (280 ng•L-1) were performed for 1 and 7 days. Metabolomics analyses evidenced that the co-exposure was the most disturbing after 7 days, reducing the amount of 25 metabolites involved in protein synthesis, energy metabolism, antioxidant response and osmoregulation, and significantly altering cells and organelles' structure supporting a reduction of functions of gills and digestive glands. CBZ alone after 7 days decreased the amount of α-aminobutyric acid and had a moderate effect on the structure of mitochondria in digestive glands. MeHg alone had no effect on mussels' metabolome, but caused a significant alteration of cells and organelles' structure in gills and digestive glands. Single exposures and the co-exposure increased antioxidant responses vs control in gills and digestive glands, without resulting in lipid peroxidation, suggesting an increased ROS production caused by both pollutants. Data globally supported that a higher number of hyperactive cells compensated cellular alterations in the digestive gland of mussels exposed to CBZ or MeHg alone, while CBZ+MeHg co-exposure overwhelmed this compensation after 7 days. Those effects were unpredictable based on cellular responses to CBZ and MeHg alone, highlighting the need to consider molecular toxicity pathways for a better anticipation of effects of pollutants in biota in complex environmental conditions.
Published: 2022

40. Synthesis and crystal structure of a zinc(II) coordination polymer: N-(2-pyridylmethyl)- L-alanine)isothiocyanate Zn(II)

Author: James Raftery, P. O’brien, Aminou Mohamadou, Evans N. Mainsah, Peter T. Ndifon, Sally-Judith E. Ntum, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Ligand, Coordination polymer, Infrared spectroscopy, chemistry.chemical_element, General Chemistry, Crystal structure, Zinc, 010402 general chemistry, 01 natural sciences, Zinc sulfide, 0104 chemical sciences, Crystallography, chemistry.chemical_compound, chemistry, [CHIM]Chemical Sciences, Orthorhombic crystal system, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Single crystal, ComputingMilieux_MISCELLANEOUS
Abstract: The title compound, N-(2-pyridylmethyl)-L-alanine) zinc(II), [Zn(C10H11N3O2S)], a coordination polymer derived from the mixed ligands, N-(2-pyridylmethyl)-L-alanine (HL1) and ammonium thiocyanide (L2) has been synthesized and characterized by IR spectroscopy, 1H-NMR, elemental analysis, thermogravimetric analysis and single crystal x-ray structure determination.The single crystal X-ray structure analysis of the title compoundreveals a molecular polymeric compound that crystallizes in the orthorhombic crystal system and P2(1)2(1)2(1) space group. The ligand acts as a bridge between adjacent zinc(II) centres, each of which adopts a five-coordinate geometry. Zinc bonds to two N atoms and one O atom of HL1, one N atom of L2 and one O atom of another HL1 ligand. This is confirmed by IR spectroscopy. Thermogravimetric analysis shows a one-step decomposition of the complex to leave a zinc sulfide residue. KEY WORDS: Zinc(II), N-(2-pyridylmethyl)-(L)-alanine) ligand, Isothiocyanate, Coordination polymer, Single crystal structure Bull. Chem. Soc. Ethiop. 2020, 34(2), 285-293 DOI: https://dx.doi.org/10.4314/bcse.v34i2.7
Published: 2020

41. Dereplication of Natural Extracts Diluted in Glycerin: Physical Suppression of Glycerin by Centrifugal Partition Chromatography Combined with Presaturation of Solvent Signals in 13C-Nuclear Magnetic Resonance Spectroscopy

Author: Richard Roe, Stéphane Poigny, Jane Hubert, Yves Brunel, Jean-Hugues Renault, Jean-Marc Nuzillard, Marine Canton, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), NatExplore, Laboratoire de Pharmacochimie Pierre Fabre Dermo-Cosmétique, Centre de Recherche Pierre Fabre, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), and PIERRE FABRE-PIERRE FABRE
Subjects: Glycerol, glycerin, Magnetic Resonance Spectroscopy, cosmetic industry, Centrifugal partition chromatography, Pharmaceutical Science, Fractionation, Chemical Fractionation, Complex Mixtures, 01 natural sciences, Article, Analytical Chemistry, lcsh:QD241-441, centrifugal partition chromatography, natural extract, lcsh:Organic chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Tandem Mass Spectrometry, Drug Discovery, Physical and Theoretical Chemistry, Solvent system, Biological Products, Chromatography, 010405 organic chemistry, Chemistry, Plant Extracts, Organic Chemistry, 13C nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, chemical profiling, 0104 chemical sciences, Dilution, Solvent, 010404 medicinal & biomolecular chemistry, Chemistry (miscellaneous), Solvents, Molecular Medicine, Sugars, [CHIM.CHEM]Chemical Sciences/Cheminformatics, Chromatography, Liquid
Abstract: For scientific, regulatory, and safety reasons, the chemical profile knowledge of natural extracts incorporated in commercial cosmetic formulations is of primary importance. Many extracts are produced or stabilized in glycerin, a practice which hampers their characterization. This article proposes a new methodology for the quick identification of metabolites present in natural extracts when diluted in glycerin. As an extension of a 13C nuclear magnetic resonance (NMR) based dereplication process, two complementary approaches are presented for the chemical profiling of natural extracts diluted in glycerin: A physical suppression by centrifugal partition chromatography (CPC) with the appropriate biphasic solvent system EtOAc/CH3CN/water 3:3:4 (v/v/v) for the crude extract fractionation, and a spectroscopic suppression by presaturation of 13C-NMR signals of glycerin applied to glycerin containing fractions. This innovative workflow was applied to a model mixture containing 23 natural metabolites. Dereplication by 13C-NMR was applied either on the dry model mixture or after dilution at 5% in glycerin, for comparison, resulting in the detection of 20 out of 23 compounds in the two model mixtures. Subsequently, a natural extract of Cedrus atlantica diluted in glycerin was characterized and resulted in the identification of 12 metabolites. The first annotations by 13C-NMR were confirmed by two-dimensional NMR and completed by LC-MS analyses for the annotation of five additional minor compounds. These results demonstrate that the application of physical suppression by CPC and presaturation of 13C-NMR solvent signals highly facilitates the quick chemical profiling of natural extracts diluted in glycerin.
Published: 2020

42. Anti-Cancer Activity of Curcumin on Multiple Myeloma

Author: Faezeh Ghasemi, Mohammad Hossein Pourhanifeh, Hossein Bagheri, Philippe Jeandet, Yvan Vander Heyden, Hamed Mirzaei, Haroon Khan, Erfan Mortezapour, Amirhossein Sahebkar, Ali Nikdasti, Jaber M. Khoi, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, and Pharmaceutical and Pharmacological Sciences
Subjects: CRAB features, Curcumin, HRQoL, multidrug resistance, multiple myeloma, therapy, Cancer Research, Cell cycle checkpoint, Cell Survival, [SDV]Life Sciences [q-bio], 03 medical and health sciences, chemistry.chemical_compound, Curcuma, 0302 clinical medicine, medicine, Humans, Multiple myeloma, Cell Proliferation, 030304 developmental biology, Pharmacology, 0303 health sciences, Molecular Structure, business.industry, Cell Cycle, Cancer, medicine.disease, Antineoplastic Agents, Phytogenic, 3. Good health, medicine.anatomical_structure, chemistry, Apoptosis, 030220 oncology & carcinogenesis, Monoclonal, Cancer research, Molecular Medicine, Bone marrow, Drug Screening Assays, Antitumor, Signal transduction, Multiple Myeloma, business
Abstract: Multiple Myeloma (MM) is the third most common and deadly hematological malignancy, which is characterized by a progressive monoclonal proliferation within the bone marrow. MM is cytogenetically heterogeneous with numerous genetic and epigenetic alterations, which lead to a wide spectrum of signaling pathways and cell cycle checkpoint aberrations. MM symptoms can be attributed to CRAB features (hyperCalcemia, Renal failure, Anemia, and Bone lesion), which profoundly affect both the Health-Related Quality of Life (HRQoL) and the life expectancy of patients. Despite all enhancement and improvement in therapeutic strategies, MM is almost incurable, and patients suffering from this disease eventually relapse. Curcumin is an active and non-toxic phenolic compound, isolated from the rhizome of Curcuma longa L. It has been widely studied and has a confirmed broad range of therapeutic properties, especially anti-cancer activity, and others, including anti-proliferation, anti-angiogenesis, antioxidant and anti-mutation activities. Curcumin induces apoptosis in cancerous cells and prevents Multidrug Resistance (MDR). Growing evidence concerning the therapeutic properties of curcumin caused a pharmacological impact on MM. It is confirmed that curcumin interferes with various signaling pathways and cell cycle checkpoints, and with oncogenes. In this paper, we summarized the anti- MM effects of curcumin.
Published: 2020

43. Biosurfactants in Plant Protection Against Diseases: Rhamnolipids and Lipopeptides Case Study

Author: Jérôme, Crouzet, Anthony, Arguelles-Arias, Sandrine, Dhondt-Cordelier, Sylvain, Cordelier, Jelena, Pršić, Gregory, Hoff, Florence, Mazeyrat-Gourbeyre, Fabienne, Baillieul, Christophe, Clément, Marc, Ongena, Stéphan, Dorey, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Gembloux Agro-Bio Tech, TERRA Research Center, Microbial Processes and Interactions (MiPI), and Université de Liège
Subjects: elicitor, lcsh:Biotechnology, lcsh:TP248.13-248.65, [SDV]Life Sciences [q-bio], fungi, antimicrobial, rhamnolipids, plant immunity, lipopeptides, plant pathogen
Abstract: International audience; Biosurfactants are amphiphilic surface-active molecules that are produced by a variety of microorganisms including fungi and bacteria. Pseudomonas, Burkholderia and Bacillus species are known to secrete rhamnolipids and lipopeptides that are used in a wide range of industrial applications. Recently, these compounds have been studied in a context of plant-microbe interactions. This mini-review describes the direct antimicrobial activities of these compounds against plant pathogens. We also provide the current knowledge on how rhamnolipids and lipopeptides stimulate the plant immune system leading to plant resistance to phytopathogens. Given their low toxicity, high biodegradability and ecological acceptance, we discuss the possible role of these biosurfactants as alternative strategies to reduce or even replace pesticide use in agriculture.
Published: 2020

44. Revisiting the Amyloid Cascade Hypothesis: From Anti-Aβ Therapeutics to Auspicious New Ways for Alzheimer’s Disease

Author: Sohanur Rahman, Mohamed M. Abdel-Daim, Ghulam Md Ashraf, Hesham R. El-Seedi, May Bin-Jumah, Tanvir Kabir, Philippe Jeandet, Tapan Behl, Agnieszka Najda, Sahab Uddin, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Neurology, Neurologi, [SDV]Life Sciences [q-bio], Peptide, Disease, Review, amyloid precursor protein, Pharmacology, lcsh:Chemistry, 0302 clinical medicine, Amyloid precursor protein, tau, lcsh:QH301-705.5, Spectroscopy, Aβ, chemistry.chemical_classification, 0303 health sciences, biology, Biochemistry and Molecular Biology, aducanumab, General Medicine, Alzheimer's disease, 3. Good health, Computer Science Applications, BAN2401, Aducanumab, Amyloid cascade, Alzheimer’s disease, medicine.medical_specialty, Amyloid, medicine.drug_class, tau Proteins, A beta, Monoclonal antibody, Fibril, Models, Biological, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Alzheimer Disease, medicine, Animals, Humans, Physical and Theoretical Chemistry, Molecular Biology, 030304 developmental biology, Amyloid beta-Peptides, business.industry, Organic Chemistry, chemistry, lcsh:Biology (General), lcsh:QD1-999, biology.protein, business, 030217 neurology & neurosurgery, Biokemi och molekylärbiologi, Biomarkers
Abstract: International audience; Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder related to age, characterized by the cerebral deposition of fibrils, which are made from the amyloid-β (Aβ), a peptide of 40–42 amino acids. The conversion of Aβ into neurotoxic oligomeric, fibrillar, and protofibrillar assemblies is supposed to be the main pathological event in AD. After Aβ accumulation, the clinical symptoms fall out predominantly due to the deficient brain clearance of the peptide. For several years, researchers have attempted to decline the Aβ monomer, oligomer, and aggregate levels, as well as plaques, employing agents that facilitate the reduction of Aβ and antagonize Aβ aggregation, or raise Aβ clearance from brain. Unluckily, broad clinical trials with mild to moderate AD participants have shown that these approaches were unsuccessful. Several clinical trials are running involving patients whose disease is at an early stage, but the preliminary outcomes are not clinically impressive. Many studies have been conducted against oligomers of Aβ which are the utmost neurotoxic molecular species. Trials with monoclonal antibodies directed against Aβ oligomers have exhibited exciting findings. Nevertheless, Aβ oligomers maintain equivalent states in both monomeric and aggregation forms; so, previously administered drugs that precisely decrease Aβ monomer or Aβ plaques ought to have displayed valuable clinical benefits. In this article, Aβ-based therapeutic strategies are discussed and several promising new ways to fight against AD are appraised.
Published: 2020

45. Synthetic Mono-Rhamnolipids Display Direct Antifungal Effects and Trigger an Innate Immune Response in Tomato against Botrytis Cinerea

Author: Robineau, Mathilde, Le Guenic, Sarah, Sanchez, Lisa, Chaveriat, Ludovic, Lequart, Vincent, Joly, Nicolas, Calonne, Maryline, Jacquard, Cédric, Declerck, Stéphane, Martin, Patrick, Dorey, Stephan, Ait Barka, Essaid, Unité Transformations & Agroressources [Université d'Artois] (UTA), Université d'Artois (UA)-Transformations et Agro-ressources (UT&A), UniLaSalle-UniLaSalle, Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Earth and Life Institute [Louvain-La-Neuve] (ELI), and Université Catholique de Louvain = Catholic University of Louvain (UCL)
Subjects: [CHIM.ORGA]Chemical Sciences/Organic chemistry, Botrytis, fungi, food and beverages, [CHIM.MATE]Chemical Sciences/Material chemistry, tomato, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, induced resistance, [SPI.MAT]Engineering Sciences [physics]/Materials, lcsh:QD241-441, [CHIM.POLY]Chemical Sciences/Polymers, lcsh:Organic chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, elicitors, innate immunity, ComputingMilieux_MISCELLANEOUS, [SPI.GCIV.EC]Engineering Sciences [physics]/Civil Engineering/Eco-conception
Abstract: International audience; Natural rhamnolipids are potential biocontrol agents for plant protection against bacterial and fungal diseases. In this work, we synthetized new synthetic mono-rhamnolipids (smRLs) consisting in a rhamnose connected to a simple acyl chain and differing by the nature of the link and the length of the lipid tail. We then investigated the effects of these ether, ester, carbamate or succinate smRL derivatives on Botrytis cinerea development, symptoms spreading on tomato leaves and immune responses in tomato plants. Our results demonstrate that synthetic smRLs are able to trigger early and late immunity-related plant defense responses in tomato and increase plant resistance against B. cinerea in controlled conditions. Structure-function analysis showed that chain length of the lipidic part and type of acyl chain were critical to smRLs immune activity and to the extent of symptoms caused by the fungus on tomato leaves.
Published: 2020

46. Électricité et lumière en synthèse organique : l’électrophotocatalyse

Author: Corentin Lefebvre, Lucas Fortier, Norbert Hoffmann, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institute of Condensed Matter and Nanoscience (IMCN), Institute of condenced Matter and Nanoscience (IMCN), Université de Lille, Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Organic electrochemistry, electrophotocatalysis, organic photochemistry, cation-radical, [CHIM.ORGA]Chemical Sciences/Organic chemistry, [CHIM]Chemical Sciences, organic synthesis, anion- radical
Abstract: International audience; Electricity and light in organic synthesis: electrophotocatalysisFor a long time neglected to activation process or deprotection reactions, photochemical and electrochemical methods undergo a strong growth in organic synthesis these last years. More specifically, photoredox catalysis reactions are more and more used. In these latter case, light energy absorption by a metallic complex or an organic molecule allows exalting of their redox properties: the molecule becomes a stronger oxidant and reductive species. As part of these reactions, an electron transfer initiates a catalytic process. It is also possible to directly inject electrons into the reaction mixture via an electrical source without the use of light or a catalyst to start a chemical reaction: it is called organic electrochemistry. These two methods, although different in their practical implementation, enable the access to similar molecular structures through comparable mechanism steps. Some examples are presented, notably the Kolbe reaction and the alkene hydroamidation. Combination of electrochemistry and photochemistry in organic synthesis is also presented via the conception of new catalysts bearing great oxidation and reduction potentials, with their applications in organic chemistry.; Longtemps laissées au rang de processus d’activation ou de réactions de déprotection, les méthodes photo- chimiques et électrochimiques connaissent un véritable essor en synthèse organique ces dernières années. Plus particulièrement, les réactions dites de catalyse photoredox sont de plus en plus utilisées. Dans ces réactions, l’absorption de l’énergie lumineuse par un catalyseur métallique ou organique permet d’exalter leurs propriétés redox : ce dernier devient un meilleur oxydant et un meilleur réducteur. Dans le cadre de ces réactions, un transfert d’électron permet d’amorcer le processus catalytique. Il est également possible d’injecter directement les électrons dans le milieu réactionnel par une source électrique, sans utiliser de lumière ou de catalyseur pour déclencher une réaction chimique : on parle alors d’électrochimie organique. Ces deux méthodes, différentes du point de vue de la mise en œuvre, permettent néanmoins d’accéder à des structures moléculaires similaires impli- quant des étapes de mécanisme comparables. Quelques exemples sont présentés dans cet article, dont la réaction de Kolbe et l’hydroamidation d’alcènes. La combinaison électrochimie/photochimie en synthèse organique est également mentionnée avec la conception de nouveaux catalyseurs présentant des potentiels d’oxydation et de réduction élevés et leurs applications en chimie organique.
Published: 2020

47. Performance criteria for the verification of IgE and tryptase assay methods: recommendations from the AllergoBioNet network

Author: M. Vivinus, Marie-Alexandra Alyanakian, P.A. Apoil, Arnaud Cirée, Anthony Léon, Angélique Grenier, Pascale Nicaise-Roland, Thierry Tabary, Valérie Guérin, Stéphanie Rogeau, Anne Sarrat, Béatrice Uring-Lambert, Martine Pernollet, Lorna Garnier, Pascale Chrétien, Delphine Mariotte, Remy Couderc, Erwan Dumontet, Bertrand Evrard, Benoît Cypriani, Lionel Chollet, Caroline Hémont, Joana Vitte, Céline Beauvillain, CHU Bordeaux [Bordeaux], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Immunologie biologique [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier Intercommunal de Toulon - La Seyne-sur-Mer, Hôpitaux Universitaires Paris Sud [AP-HP] (HUPS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Service de biochimie médicale [CHRU Besançon, hôpital Minjoz], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Microenvironment, Cell Differentiation, Immunology and Cancer (MICMAC), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Biologie intégrative des organismes marins (BIOM), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Unité Fonctionnelles d′Immunologie [CHU Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Les Hôpitaux Universitaires de Strasbourg (HUS), Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA), Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Archet 2 [Nice] (CHU), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Service d'Allergologie pédiatrique [CHU Trousseau], CHU Toulouse [Toulouse], Hôpitaux Universitaires Paris Sud, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)
Subjects: medicine.medical_specialty, Consensus, Computer science, media_common.quotation_subject, [SDV]Life Sciences [q-bio], tryptase, Fidelity, Tryptase, Immunoglobulin E, In vitro diagnostic, Accreditation, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Medical physics, quality control, 030304 developmental biology, media_common, 0303 health sciences, biology, Diagnostic Tests, Routine, Total ige, Reproducibility of Results, specific IgE antibodies, General Medicine, Repeatability, allergy, 3. Good health, total IgE, Technical performance, 030228 respiratory system, biology.protein, Measurement uncertainty, Biological Assay, Tryptases, France, Laboratories
Abstract: International audience; Accreditation of an in vitro diagnostic assay according to the NHENI/ISO 15189 standard requires to analyze its technical performance before implementation for routine use, and annually when reviewing effectiveness of quality controls. Performance is evaluated through repeatability, intermediate fidelity, accuracy and uncertainty of measurement. The coefficients of variation (CV) of the infra-assay and inter-assay precision tests must be compared with those of ``peers'' (results from laboratories employing the same method) and also with those obtained with ``all methods'', i.e., results from all laboratories performing the same assay, irrespective of the method. To our best knowledge, there is currently no French or international recommendation on what the acceptable limits of performance for specific IgE and tryptase assays should be. Therefore, the AllergoBioNet network of hospital allergy laboratories set out to characterize the performance of their current methods as a basis for the development of recommendations. The results provided by 24 centers were analyzed and led to consensus recommendations for specific IgE, total and tryptase assays.
Published: 2020

48. An optimal waterline approach for studying tidal flat morphological changes using remote sensing data: A case of the northern coast of Vietnam

Author: Jean-Paul Deroin, Thi Lan Pham, Si Son Tong, Groupe d'Étude sur les Géomatériaux et Environnements Naturels, Anthropiques et Archéologiques - EA 3795 (GEGENAA), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Maison des Sciences Humaines de Champagne-Ardenne (MSH-URCA), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA), SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 010504 meteorology & atmospheric sciences, Morphological change, Aquatic Science, Oceanography, 01 natural sciences, Deposition (geology), Normalized Difference Vegetation Index, Waterline, symbols.namesake, Advanced Spaceborne Thermal Emission and Reflection Radiometer, Tidal flats, Gulf of tonkin, River mouth, 14. Life underwater, Digital elevation model, Geomorphology, 0105 earth and related environmental sciences, geography, geography.geographical_feature_category, 010604 marine biology & hydrobiology, Waterlines extraction, Spectral bands, 13. Climate action, [SDU]Sciences of the Universe [physics], symbols, Erosion, Landsat, Geology
Abstract: International audience; Tidal flats on the north coast of Vietnam suffer diurnal tide with a tide range varying between 0.3 m and 3.5 m. Along the 350 km long coastline, the diversity of environmental conditions induces various tidal flats with different characteristics. This study applies the waterline method for multi-temporal satellite images to build Digital Elevation Models (DEMs) of tidal flats during the last 25 years. 117 Landsat images acquired with TM, ETM+, and OLI have been processed to construct tidal flat DEMs in 1989, 2000, and 2014. Waterlines extracted from single spectral bands (near-infrared [NIR], short wave infrared [SWIR]) or band ratios (normalized difference water index [NDWI], normalized difference vegetation index [NDVI], Green/SWIR) of the Landsat data have been compared with waterlines digitalized on Spot, Aster and Worldview 2 images. This experiment allows us to determine the best band (or band ratio) for extracting waterlines depending on local conditions. Consequently, the study shows that the Green/SWIR ratio image is a good solution for extracting waterlines in the black coal tidal flats of Cam Pha. However, the NDWI index appears to be a better choice for the other parts of the study area. The vertical accuracy of the tidal flat DEMs reaches 0.144 m. The change analysis of the DEMs also emphasizes the tidal flat evolution in both vertical and horizontal dimensions, i.e. erosion or accretion. The erosion of the tidal flats along the northern coast of Vietnam is particularly developed in the area extending from Yen Hung to Mong Cai, especially in Mong Cai with an amount of about 50 × 106 m3 of sediments lost between 1989 and 2014. On the contrary, the tidal flats in the south of the study area show a high rate of deposition due to the sediments fed by Red and Thai Binh rivers. About 35 × 106 m3 of sediments deposited in the tidal flat surrounding the Red River mouth between 1989 and 2014. This study represents a development of the waterline extraction method to investigate the evolution of tidal flat at a large scale and a diversified coastal environment using optical satellite images and fieldwork.
Published: 2020

49. Mixture Analysis in Viscous Solvents by NMR Spin Diffusion Spectroscopy: ViscY. Application to High- and Low-Polarity Organic Compounds Dissolved in Sulfolane/Water and Sulfolane/DMSO-d 6 Blends

Author: François Pedinielli, Jean-Marc Nuzillard, Pedro Lameiras, Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Chemistry, 010401 analytical chemistry, Nuclear magnetic resonance spectroscopy, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Solvent, chemistry.chemical_compound, Heteronuclear molecule, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Spin diffusion, Physical chemistry, Molecule, Magnetization transfer, Sulfolane, Two-dimensional nuclear magnetic resonance spectroscopy
Abstract: International audience; The sulfolane/water and sulfolane/DMSO-d6 binary NMR solvents are reported for the first time for the individual-ization of mixture components by spin diffusion, when molecular tumbling is slow due to solvent viscosity, thus strongly favouring magnetization transfer by dipolar cross relaxation. All 1 H nuclei resonances within the same molecule tend to correlate in a 2D NOESY spectrum, opening the way to mixture analysis. Till now, analysis of organic compounds by NMR spin diffusion in viscous solvents involved 1 H, 13 C, 15 N and 19 F.We offer a new way to analyse mixtures by considering 31 P nuclei as chemical shift markers. We report the individualization of four polar dipeptides and of four non-polar phosphorus-containing compounds respectively dissolved in sulfolane/water and sulfolane/DMSO-d6 solvents blends by means of homonuclear selective 1D and 2D 1 H experiments and a heteronuclear 2D 1 H-31 P HSQC-NOESY experiment by taking advantage from spin diffusion. The name ViscY is proposed to refer to the class of all NMR spectroscopy experiments that rely on viscous solvents for mixture analysis.
Published: 2020

50. Mechanical and hygrothermal characteri s ation of cork concrete composite: experimental and modelling study

Author: Hocine Boussetoua, Mohammed Lachi, Tala Moussa, Azzedine Belhamri, Chadi Maalouf, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Groupe de Recherche en Sciences Pour l'Ingénieur - EA 4694 (GRESPI), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Matériaux et Ingénierie Mécanique (MATIM), and Université de Reims Champagne-Ardenne (URCA)
Subjects: Cement, Environmental Engineering, Materials science, Moisture, Composite number, 0211 other engineering and technologies, Mixing (process engineering), 02 engineering and technology, Cork, engineering.material, [SPI]Engineering Sciences [physics], 021105 building & construction, engineering, Composite material, 021101 geological & geomatics engineering, Civil and Structural Engineering
Abstract: International audience; The paper aims to present a new insulating material based on cork aggregates and cement designed for building applications. Samples are prepared by mixing natural cork aggregates, sand, cement and water. Four cork volume dosages are considered, 0, 25, 50 and 75% (relative to the sand). Samples are characterised in terms of hardened density, compressive and flexural strength, as well as thermal conductivity and hydric properties (moisture content, vapour permeability, moisture buffering value and absorption by partial immersion). Results show that increasing cork granule amount tends to increase moisture retention and buffering value but decreases density, thermal conductivity and mechanical properties. Depending on the cork content, cork concrete mixture can be used as thermal insulator or as a structural component.
Published: 2020

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